Dppa5a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dppa5a
Entrez Gene ID	434423
Full Name	developmental pluripotency associated 5A
Synonyms	AA536857,Dppa5,Esg1,ecat2
General protein information	Preferred Names developmental pluripotency associated 5A Names developmental pluripotency-associated protein 5A ES cell-associated transcript 2 protein ESG-1 developmental pluripotency associated 5 embryonal stem cell specific gene 1 embryonal stem cell-specific gene 1 protein mDppa5 protein pH 34
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_025274.3	NP_079550.2	developmental pluripotency-associated protein 5A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DPPA5	XP_003311448.1
Homo sapiens (human)	DPPA5	NP_001020461.1
	LOC715759	XP_001112340.1
Canis lupus familiaris (dog)	DPPA5	XP_003639485.1
Mus musculus (house mouse)	Dppa5a	NP_079550.2

Gene Ontology
Bibliography

Related articles in PubMed

Essential role of the TFIID subunit TAF4 in murine embryogenesis and embryonic stem cell differentiation.
Langer D, Martianov I, Alpern D, Rhinn M, Keime C, Doll? P, Mengus G, Davidson I
Nature communications711063(2016 Mar)

Interaction of NANOS2 and NANOS3 with different components of the CNOT complex may contribute to the functional differences in mouse male germ cells.
Suzuki A, Niimi Y, Saga Y
Biology open3(12)1207-16(2014 Nov)

Dppa5 improves hematopoietic stem cell activity by reducing endoplasmic reticulum stress.
Miharada K, Sigurdsson V, Karlsson S
Cell reports7(5)1381-1392(2014 Jun)

Interaction between DMRT1 function and genetic background modulates signaling and pluripotency to control tumor susceptibility in the fetal germ line.
Krentz AD, Murphy MW, Zhang T, Sarver AL, Jain S, Griswold MD, Bardwell VJ, Zarkower D
Developmental biology377(1)67-78(2013 May)

Placental PPAR? regulates spatiotemporally diverse genes and a unique metabolic network.
Shalom-Barak T, Zhang X, Chu T, Timothy Schaiff W, Reddy JK, Xu J, Sadovsky Y, Barak Y
Developmental biology372(1)143-55(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dppa5 reduces endoplasmic reticulum stress in hematopoietic stem cells.
Title: Dppa5 improves hematopoietic stem cell activity by reducing endoplasmic reticulum stress.

ESG1 is an RNA-binding protein which associates with specific target transcripts such as Cdc25a, Cdc42, Ezh2, Nfyc and Nr5a2.
Title: Esg1, expressed exclusively in preimplantation embryos, germline, and embryonic stem cells, is a putative RNA-binding protein with broad RNA targets.

Despite its specific expression in pluripotent cells and germ cells, ESG1 is dispensable for self-renewal of ES cells and establishment of germ cells.
Title: Identification and targeted disruption of the mouse gene encoding ESG1 (PH34/ECAT2/DPPA5).

developmental pluripotency associated 5 (Dppa5) was strongly expressed in mouse PGCs and the expression was rapidly downregulated during germ cell development.
Title: Identification of developmental pluripotency associated 5 expression in human pluripotent stem cells.

The following Dppa5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dppa5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22515	NM_025274.3 Latest version!	Mus musculus developmental pluripotency associated 5A (Dppa5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dppa5a ( NM_025274.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079550.2 Mus musculus developmental pluripotency associated 5A (Dppa5a), mRNA.

Price & Availability↑

CloneID	OMu22515
Clone ID Related Accession (Same CDS sequence)	NM_025274.3
Accession Version	NM_025274.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	developmental pluripotency-associated protein 5A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AV457404.1 and AV476247.1. On Jan 12, 2008 this sequence version replaced NM_025274.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC092353.1, AK145761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849383 [ECO:0000348] ##Evidence-Data-END##

ATGATGGTGA CCCTCGTGAC CCGTAAAGAT ATCCCCCCGT GGGTGAAAGT TCCTGAAGAC 
CTGAAAGATC CAGAAGTATT CCAGGTCCAG TCGCTGGTGC TGAAATATCT GTTTGGCCCA 
CAGGGATCTC GAATGTCTCA CATCGAGCAG GTGAGCCAGG CCATGTTTGA GCTGAAGAAC 
CTGGAATCTC CCGAAGAACT TATCGAGGTC TTCATTTACG GCTCTCAAAA CAACAAGATT 
CGGGCTAAAT GGATGCTTCA GTCCATGGCT GAGAGGTACC ACCTGCGCCA GCAAAAAGGA 
GTGCTGAAGC TGGAGGAATC CATGAAGACC CTGGAGCTAG GCCAGTGTAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079550.2
CDS	101..457
Translation	MMVTLVTRKDIPPWVKVPEDLKDPEVFQVQSLVLKYLFGPQGSRMSHIEQVSQAMFELKNLESPEELIEVFIYGSQNNKIRAKWMLQSMAERYHLRQQKGVLKLEESMKTLELGQCIE

Target ORF information:

RefSeq Version	NM_025274.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus developmental pluripotency associated 5A (Dppa5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025274.3

ORF Insert Sequence:

ATGATGGTGA CCCTCGTGAC CCGTAAAGAT ATCCCCCCGT GGGTGAAAGT TCCTGAAGAC 
CTGAAAGATC CAGAAGTATT CCAGGTCCAG TCGCTGGTGC TGAAATATCT GTTTGGCCCA 
CAGGGATCTC GAATGTCTCA CATCGAGCAG GTGAGCCAGG CCATGTTTGA GCTGAAGAAC 
CTGGAATCTC CCGAAGAACT TATCGAGGTC TTCATTTACG GCTCTCAAAA CAACAAGATT 
CGGGCTAAAT GGATGCTTCA GTCCATGGCT GAGAGGTACC ACCTGCGCCA GCAAAAAGGA 
GTGCTGAAGC TGGAGGAATC CATGAAGACC CTGGAGCTAG GCCAGTGTAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Essential role of the TFIID subunit TAF4 in murine embryogenesis and embryonic stem cell differentiation.
Nature communications711063(2016 Mar)
Langer D,Martianov I,Alpern D,Rhinn M,Keime C,Doll? P,Mengus G,Davidson I

Interaction of NANOS2 and NANOS3 with different components of the CNOT complex may contribute to the functional differences in mouse male germ cells.
Biology open3(12)1207-16(2014 Nov)
Suzuki A,Niimi Y,Saga Y

Dppa5 improves hematopoietic stem cell activity by reducing endoplasmic reticulum stress.
Cell reports7(5)1381-1392(2014 Jun)
Miharada K,Sigurdsson V,Karlsson S

Interaction between DMRT1 function and genetic background modulates signaling and pluripotency to control tumor susceptibility in the fetal germ line.
Developmental biology377(1)67-78(2013 May)
Krentz AD,Murphy MW,Zhang T,Sarver AL,Jain S,Griswold MD,Bardwell VJ,Zarkower D

Placental PPAR? regulates spatiotemporally diverse genes and a unique metabolic network.
Developmental biology372(1)143-55(2012 Dec)
Shalom-Barak T,Zhang X,Chu T,Timothy Schaiff W,Reddy JK,Xu J,Sadovsky Y,Barak Y