gRNAs
The following ESPNL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESPNL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu02424 | |
| Clone ID Related Accession (Same CDS sequence) | NM_194312.3 , NM_194312.4 | |
| Accession Version | NM_194312.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-02 | |
| Organism | Homo sapiens(human) | |
| Product | espin-like protein isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC016757.10, AK124559.1, AK128187.1 and BC080639.1. On May 5, 2015 this sequence version replaced NM_194312.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK124559.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGAGAAGC AGCGGGCACT CGTGGCCGCC AAGGATGGGG ATGTGGCGAC GTTGGAGCGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_919288.2 |
| CDS | 111..3128 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_194312.3 |
1 | ATGGAGAAGC AGCGGGCACT CGTGGCCGCC AAGGATGGGG ATGTGGCGAC GTTGGAGCGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu02424 | |
| Clone ID Related Accession (Same CDS sequence) | NM_194312.3 , NM_194312.4 | |
| Accession Version | NM_194312.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-30 | |
| Organism | Homo sapiens(human) | |
| Product | espin-like protein isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC016757.10, AK124559.1, AK128187.1 and BC080639.1. On Nov 22, 2018 this sequence version replaced NM_194312.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK124559.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000343063.8/ ENSP00000339115.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. | |
1 | ATGGAGAAGC AGCGGGCACT CGTGGCCGCC AAGGATGGGG ATGTGGCGAC GTTGGAGCGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_919288.2 |
| CDS | 81..3098 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_194312.4 |
1 | ATGGAGAAGC AGCGGGCACT CGTGGCCGCC AAGGATGGGG ATGTGGCGAC GTTGGAGCGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu36188 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001308370.1 | |
| Accession Version | NM_001308370.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-06-30 | |
| Organism | Homo sapiens(human) | |
| Product | espin-like protein isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC080639.1, BC042051.1, AK128187.1 and AC016757.10. On May 5, 2015 this sequence version replaced XM_005246073.2. Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC080639.1, BU848792.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTCCCTCA GCCCGGCCTG GCCTGGCCAT CCTGACCAGC CTCTTCCCAG GGAGCAGATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001295299.1 |
| CDS | 142..2055 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001308370.1 |
1 | ATGTCCCTCA GCCCGGCCTG GCCTGGCCAT CCTGACCAGC CTCTTCCCAG GGAGCAGATG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu79278 | |
| Clone ID Related Accession (Same CDS sequence) | XM_011511087.1 | |
| Accession Version | XM_011511087.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | espin-like protein isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGCAGGT GCCGGCAGGA CAATGGTGGG AGCTCAGGCC CCACGGAGCA GGCGGCCTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_011509389.1 |
| CDS | 277..1899 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_011511087.1 |
1 | ATGGGCAGGT GCCGGCAGGA CAATGGTGGG AGCTCAGGCC CCACGGAGCA GGCGGCCTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Stereocilia-staircase spacing is influenced by myosin III motors and their cargos espin-1 and espin-like. |
The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. |
