HLA-DMA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HLA-DMA
Entrez Gene ID	3108
Full Name	major histocompatibility complex, class II, DM alpha
Synonyms	D6S222E,DMA,HLADM,RING6
General protein information	Preferred Names major histocompatibility complex, class II, DM alpha Names HLA class II histocompatibility antigen, DM alpha chain MHC class II antigen DMA class II histocompatibility antigen, M alpha chain really interesting new gene 6 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.32
Summary	HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. [provided by RefSeq, Jul 2008].
Disorder MIM:	142855

mRNA and Protein(s)

mRNA	Protein	Name
NM_006120.4	NP_006111.2	HLA class II histocompatibility antigen, DM alpha chain precursor
NM_006120.3	NP_006111.2	HLA class II histocompatibility antigen, DM alpha chain precursor

Pathways from BioSystems

Homologies

	MAMU-DMA	XP_002803735.1
Homo sapiens (human)	HLA-DMA	NP_006111.2
Bos taurus (cattle)	BOLA-DMA	NP_001012692.2
Pan troglodytes (chimpanzee)	MAMU-DMA	XP_003311254.1
Mus musculus (house mouse)	H2-DMa	NP_034516.3
Gallus gallus (chicken)	BMA1	NP_001092823.1

Gene Ontology
Bibliography

Related articles in PubMed

pH-susceptibility of HLA-DO tunes DO/DM ratios to regulate HLA-DM catalytic activity.
Jiang W, Strohman MJ, Somasundaram S, Ayyangar S, Hou T, Wang N, Mellins ED
Scientific reports517333(2015 Nov)

HLA-DMA polymorphisms differentially affect MHC class II peptide loading.
?lvaro-Benito M, Wieczorek M, Sticht J, Kipar C, Freund C
Journal of immunology (Baltimore, Md. : 1950)194(2)803-16(2015 Jan)

HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism.
Guce AI, Mortimer SE, Yoon T, Painter CA, Jiang W, Mellins ED, Stern LJ
Nature structural & molecular biology20(1)90-8(2013 Jan)

Crystal structure of the HLA-DM-HLA-DR1 complex defines mechanisms for rapid peptide selection.
Pos W, Sethi DK, Call MJ, Schulze MS, Anders AK, Pyrdol J, Wucherpfennig KW
Cell151(7)1557-68(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

pH-susceptibility of HLA-DO tunes DO/DM ratios to regulate HLA-DM catalytic activity.
Title: pH-susceptibility of HLA-DO tunes DO/DM ratios to regulate HLA-DM catalytic activity.

study therefore reveals that HLA-DM polymorphic residues have a considerable impact on HLA-DM catalytic activity.
Title: HLA-DMA polymorphisms differentially affect MHC class II peptide loading.

The structure of the HLA-DM-HLA-DR complex shows major rearrangements of the HLA-DR peptide-binding groove.
Title: Crystal structure of the HLA-DM-HLA-DR1 complex defines mechanisms for rapid peptide selection.

DMA*01:01 (69.35%) and DMB*01:01 (52.5%) alleles were more frequent in Chinese Hans
Title: Analysis of HLA-DM polymorphisms in the Chinese Han population.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

The following HLA-DMA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLA-DMA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18930	NM_006120.4 Latest version!	Homo sapiens major histocompatibility complex, class II, DM alpha (HLA-DMA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu18930	NM_006120.3	Homo sapiens major histocompatibility complex, class II, DM alpha (HLA-DMA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HLA-DMA ( NM_006120.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006111.2 Homo sapiens major histocompatibility complex, class II, DM alpha (HLA-DMA), mRNA.

Price & Availability↑

CloneID	OHu18930
Clone ID Related Accession (Same CDS sequence)	NM_006120.4 , NM_006120.3
Accession Version	NM_006120.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	HLA class II histocompatibility antigen, DM alpha chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU100342.1, BC011447.1 and AA649861.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_006120.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189664.121619.1, SRR5189655.179218.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000374843.9/ ENSP00000363976.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGTCATG AACAGAACCA AGGAGCTGCG CTGCTACAGA TGTTACCACT TCTGTGGCTG 
CTACCCCACT CCTGGGCCGT CCCTGAAGCT CCTACTCCAA TGTGGCCAGA TGACCTGCAA 
AACCACACAT TCCTGCACAC AGTGTACTGC CAGGATGGGA GTCCCAGTGT GGGACTCTCT 
GAGGCCTACG ACGAGGACCA GCTTTTCTTC TTCGACTTTT CCCAGAACAC TCGGGTGCCT 
CGCCTGCCCG AATTTGCTGA CTGGGCTCAG GAACAGGGAG ATGCTCCTGC CATTTTATTT 
GACAAAGAGT TCTGCGAGTG GATGATCCAG CAAATAGGGC CAAAACTTGA TGGGAAAATC 
CCGGTGTCCA GAGGGTTTCC TATCGCTGAA GTGTTCACGC TGAAGCCCCT GGAGTTTGGC 
AAGCCCAACA CTTTGGTCTG TTTTGTCAGT AATCTCTTCC CACCCATGCT GACAGTGAAC 
TGGCAGCATC ATTCCGTCCC TGTGGAAGGA TTTGGGCCTA CTTTTGTCTC AGCTGTCGAT 
GGACTCAGCT TCCAGGCCTT TTCTTACTTA AACTTCACAC CAGAACCTTC TGACATTTTC 
TCCTGCATTG TGACTCACGA AATTGACCGC TACACAGCAA TTGCCTATTG GGTACCCCGG 
AACGCACTGC CCTCAGATCT GCTGGAGAAT GTGCTGTGTG GCGTGGCCTT TGGCCTGGGT 
GTGCTGGGCA TCATCGTGGG CATTGTTCTC ATCATCTACT TCCGGAAGCC TTGCTCAGGT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006111.2
CDS	62..847
Translation	MGHEQNQGAALLQMLPLLWLLPHSWAVPEAPTPMWPDDLQNHTFLHTVYCQDGSPSVGLSEAYDEDQLFFFDFSQNTRVPRLPEFADWAQEQGDAPAILFDKEFCEWMIQQIGPKLDGKIPVSRGFPIAEVFTLKPLEFGKPNTLVCFVSNLFPPMLTVNWQHHSVPVEGFGPTFVSAVDGLSFQAFSYLNFTPEPSDIFSCIVTHEIDRYTAIAYWVPRNALPSDLLENVLCGVAFGLGVLGIIVGIVLIIYFRKPCSGD

Target ORF information:

RefSeq Version	NM_006120.4
Organism	Homo sapiens(human)
Definition	Homo sapiens major histocompatibility complex, class II, DM alpha (HLA-DMA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006120.4

ORF Insert Sequence:

ATGGGTCATG AACAGAACCA AGGAGCTGCG CTGCTACAGA TGTTACCACT TCTGTGGCTG 
CTACCCCACT CCTGGGCCGT CCCTGAAGCT CCTACTCCAA TGTGGCCAGA TGACCTGCAA 
AACCACACAT TCCTGCACAC AGTGTACTGC CAGGATGGGA GTCCCAGTGT GGGACTCTCT 
GAGGCCTACG ACGAGGACCA GCTTTTCTTC TTCGACTTTT CCCAGAACAC TCGGGTGCCT 
CGCCTGCCCG AATTTGCTGA CTGGGCTCAG GAACAGGGAG ATGCTCCTGC CATTTTATTT 
GACAAAGAGT TCTGCGAGTG GATGATCCAG CAAATAGGGC CAAAACTTGA TGGGAAAATC 
CCGGTGTCCA GAGGGTTTCC TATCGCTGAA GTGTTCACGC TGAAGCCCCT GGAGTTTGGC 
AAGCCCAACA CTTTGGTCTG TTTTGTCAGT AATCTCTTCC CACCCATGCT GACAGTGAAC 
TGGCAGCATC ATTCCGTCCC TGTGGAAGGA TTTGGGCCTA CTTTTGTCTC AGCTGTCGAT 
GGACTCAGCT TCCAGGCCTT TTCTTACTTA AACTTCACAC CAGAACCTTC TGACATTTTC 
TCCTGCATTG TGACTCACGA AATTGACCGC TACACAGCAA TTGCCTATTG GGTACCCCGG 
AACGCACTGC CCTCAGATCT GCTGGAGAAT GTGCTGTGTG GCGTGGCCTT TGGCCTGGGT 
GTGCTGGGCA TCATCGTGGG CATTGTTCTC ATCATCTACT TCCGGAAGCC TTGCTCAGGT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLA-DMA ( NM_006120.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006111.2 Homo sapiens major histocompatibility complex, class II, DM alpha (HLA-DMA), mRNA.

Price & Availability↑

CloneID	OHu18930
Clone ID Related Accession (Same CDS sequence)	NM_006120.4 , NM_006120.3
Accession Version	NM_006120.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	HLA class II histocompatibility antigen, DM alpha chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU100342.1, AL645941.11, BC011447.1 and AA649861.1. This sequence is a reference standard in the RefSeqGene project. On Sep 30, 2008 this sequence version replaced NM_006120.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011447.1, X62744.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCATG AACAGAACCA AGGAGCTGCG CTGCTACAGA TGTTACCACT TCTGTGGCTG 
CTACCCCACT CCTGGGCCGT CCCTGAAGCT CCTACTCCAA TGTGGCCAGA TGACCTGCAA 
AACCACACAT TCCTGCACAC AGTGTACTGC CAGGATGGGA GTCCCAGTGT GGGACTCTCT 
GAGGCCTACG ACGAGGACCA GCTTTTCTTC TTCGACTTTT CCCAGAACAC TCGGGTGCCT 
CGCCTGCCCG AATTTGCTGA CTGGGCTCAG GAACAGGGAG ATGCTCCTGC CATTTTATTT 
GACAAAGAGT TCTGCGAGTG GATGATCCAG CAAATAGGGC CAAAACTTGA TGGGAAAATC 
CCGGTGTCCA GAGGGTTTCC TATCGCTGAA GTGTTCACGC TGAAGCCCCT GGAGTTTGGC 
AAGCCCAACA CTTTGGTCTG TTTTGTCAGT AATCTCTTCC CACCCATGCT GACAGTGAAC 
TGGCAGCATC ATTCCGTCCC TGTGGAAGGA TTTGGGCCTA CTTTTGTCTC AGCTGTCGAT 
GGACTCAGCT TCCAGGCCTT TTCTTACTTA AACTTCACAC CAGAACCTTC TGACATTTTC 
TCCTGCATTG TGACTCACGA AATTGACCGC TACACAGCAA TTGCCTATTG GGTACCCCGG 
AACGCACTGC CCTCAGATCT GCTGGAGAAT GTGCTGTGTG GCGTGGCCTT TGGCCTGGGT 
GTGCTGGGCA TCATCGTGGG CATTGTTCTC ATCATCTACT TCCGGAAGCC TTGCTCAGGT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006111.2
CDS	87..872
Translation	MGHEQNQGAALLQMLPLLWLLPHSWAVPEAPTPMWPDDLQNHTFLHTVYCQDGSPSVGLSEAYDEDQLFFFDFSQNTRVPRLPEFADWAQEQGDAPAILFDKEFCEWMIQQIGPKLDGKIPVSRGFPIAEVFTLKPLEFGKPNTLVCFVSNLFPPMLTVNWQHHSVPVEGFGPTFVSAVDGLSFQAFSYLNFTPEPSDIFSCIVTHEIDRYTAIAYWVPRNALPSDLLENVLCGVAFGLGVLGIIVGIVLIIYFRKPCSGD

Target ORF information:

RefSeq Version	NM_006120.3
Organism	Homo sapiens(human)
Definition	Homo sapiens major histocompatibility complex, class II, DM alpha (HLA-DMA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006120.3

ORF Insert Sequence:

ATGGGTCATG AACAGAACCA AGGAGCTGCG CTGCTACAGA TGTTACCACT TCTGTGGCTG 
CTACCCCACT CCTGGGCCGT CCCTGAAGCT CCTACTCCAA TGTGGCCAGA TGACCTGCAA 
AACCACACAT TCCTGCACAC AGTGTACTGC CAGGATGGGA GTCCCAGTGT GGGACTCTCT 
GAGGCCTACG ACGAGGACCA GCTTTTCTTC TTCGACTTTT CCCAGAACAC TCGGGTGCCT 
CGCCTGCCCG AATTTGCTGA CTGGGCTCAG GAACAGGGAG ATGCTCCTGC CATTTTATTT 
GACAAAGAGT TCTGCGAGTG GATGATCCAG CAAATAGGGC CAAAACTTGA TGGGAAAATC 
CCGGTGTCCA GAGGGTTTCC TATCGCTGAA GTGTTCACGC TGAAGCCCCT GGAGTTTGGC 
AAGCCCAACA CTTTGGTCTG TTTTGTCAGT AATCTCTTCC CACCCATGCT GACAGTGAAC 
TGGCAGCATC ATTCCGTCCC TGTGGAAGGA TTTGGGCCTA CTTTTGTCTC AGCTGTCGAT 
GGACTCAGCT TCCAGGCCTT TTCTTACTTA AACTTCACAC CAGAACCTTC TGACATTTTC 
TCCTGCATTG TGACTCACGA AATTGACCGC TACACAGCAA TTGCCTATTG GGTACCCCGG 
AACGCACTGC CCTCAGATCT GCTGGAGAAT GTGCTGTGTG GCGTGGCCTT TGGCCTGGGT 
GTGCTGGGCA TCATCGTGGG CATTGTTCTC ATCATCTACT TCCGGAAGCC TTGCTCAGGT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

pH-susceptibility of HLA-DO tunes DO/DM ratios to regulate HLA-DM catalytic activity.
Scientific reports517333(2015 Nov)
Jiang W,Strohman MJ,Somasundaram S,Ayyangar S,Hou T,Wang N,Mellins ED

HLA-DMA polymorphisms differentially affect MHC class II peptide loading.
Journal of immunology (Baltimore, Md. : 1950)194(2)803-16(2015 Jan)
?lvaro-Benito M,Wieczorek M,Sticht J,Kipar C,Freund C

HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism.
Nature structural & molecular biology20(1)90-8(2013 Jan)
Guce AI,Mortimer SE,Yoon T,Painter CA,Jiang W,Mellins ED,Stern LJ

Crystal structure of the HLA-DM-HLA-DR1 complex defines mechanisms for rapid peptide selection.
Cell151(7)1557-68(2012 Dec)
Pos W,Sethi DK,Call MJ,Schulze MS,Anders AK,Pyrdol J,Wucherpfennig KW

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HLA-DMA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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