HEXA cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HEXA
Entrez Gene ID	3073
Full Name	hexosaminidase subunit alpha
Synonyms	TSD
General protein information	Preferred Names hexosaminidase subunit alpha Names beta-hexosaminidase subunit alpha N-acetyl-beta-glucosaminidase subunit alpha beta-N-acetylhexosaminidase subunit alpha hexosaminidase A (alpha polypeptide) hexosaminidase subunit A
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 15 Map Location: 15q23
Summary	This gene encodes a member of the glycosyl hydrolase 20 family of proteins. The encoded preproprotein is proteolytically processed to generate the alpha subunit of the lysosomal enzyme beta-hexosaminidase. This enzyme, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Mutations in this gene lead to an accumulation of GM2 ganglioside in neurons, the underlying cause of neurodegenerative disorders termed the GM2 gangliosidoses, including Tay-Sachs disease (GM2-gangliosidosis type I). Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016].
Disorder MIM:	606869
Disorder Html:	Tay-Sachs disease, 272800 (3); GM2-gangliosidosis, several forms, 272800 (3); [Hex A pseudodeficiency], 272800 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001318825.2	NP_001305754.1	beta-hexosaminidase subunit alpha isoform 1 precursor
NM_000520.6	NP_000511.2	beta-hexosaminidase subunit alpha isoform 2 preproprotein
NM_000520.5	NP_000511.2	beta-hexosaminidase subunit alpha isoform 2 preproprotein
NM_001318825.1	NP_001305754.1	beta-hexosaminidase subunit alpha isoform 1 precursor
NM_000520.4	NP_000511.2	beta-hexosaminidase subunit alpha preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	HEXA	NP_000511.2
	HEXA	XP_001092589.1
Canis lupus familiaris (dog)	HEXA	XP_544758.2
Danio rerio (zebrafish)	zgc:112084	NP_001017763.1
Bos taurus (cattle)	HEXA	NP_001068632.1
Rattus norvegicus (Norway rat)	Hexa	NP_001004443.1
Gallus gallus (chicken)	HEXA	NP_001025561.1
Mus musculus (house mouse)	Hexa	NP_034551.2
Arabidopsis thaliana (thale cress)	HEXO3	NP_176737.2
Pan troglodytes (chimpanzee)	HEXA	XP_001175124.1
Oryza sativa (rice)	Os05g0415700	NP_001055557.1
Oryza sativa (rice)	Os01g0891000	NP_001045052.1

Gene Ontology
Bibliography

Related articles in PubMed

Pediatric reference data on activity of urinary N-acetyl-?-D-hexosaminidase and its isoenzymes.
Zalewska-Szajda B, Taranta-Janusz K, Chojnowska S, Waszkiewicz N, Zwierz K, Wasilewska A
Advances in medical sciences63(1)94-99(2017 Aug)

Tay-Sachs disease mutations in HEXA target the ? chain of hexosaminidase A to endoplasmic reticulum-associated degradation.
Dersh D, Iwamoto Y, Argon Y
Molecular biology of the cell27(24)3813-3827(2016 Dec)

Identification of domains in human beta-hexosaminidase that determine substrate specificity.
Pennybacker M, Liessem B, Moczall H, Tifft CJ, Sandhoff K, Proia RL
The Journal of biological chemistry271(29)17377-82(1996 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The alpha mutants E482K and G269S are defective in enzymatic activity, unprocessed by lysosomal proteases, and exhibit altered folding pathways compared with wild-type alpha. E482K is more severely misfolded than G269S, as observed by its aggregation and inability to associate with the HexA beta chain. Importantly, both mutants are retrotranslocated from the endoplasmic reticulum to the cytosol and are degraded by the ...
Title: Tay-Sachs disease mutations in HEXA target the ? chain of hexosaminidase A to endoplasmic reticulum-associated degradation.

reports a new missense mutation in the HEXA gene in two German siblings with late-onset Tay-Sachs disease and prominent psychiatric symptoms
Title: Paranoid delusion as lead symptom in two siblings with late-onset Tay-Sachs disease and a novel mutation in the HEXA gene.

Human prostate cancer cells are characterised by a significant decrease in HexA activity.
Title: Hypermethylation contributes to down-regulation of lysosomal ?-hexosaminidase ? subunit in prostate cancer cells.

DNA reveals novel mutations in ian subjects causing Tay-Sachs disease in the alpha and beta subunits of HexA.
Title: Three novel mutations in ian patients with Tay-Sachs disease.

GM2 gangliosidosis is caused by the gene mutation. (review)
Title: [Molecular pathogenesis and therapeutic approach of GM2 gangliosidosis].

The following HEXA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEXA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18820	NM_000520.6 Latest version!	Homo sapiens hexosaminidase subunit alpha (HEXA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu100681	NM_001318825.2 Latest version!	Homo sapiens hexosaminidase subunit alpha (HEXA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu18820	NM_000520.5	Homo sapiens hexosaminidase subunit alpha (HEXA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu100681	NM_001318825.1	Homo sapiens hexosaminidase subunit alpha (HEXA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu18820	NM_000520.4	Homo sapiens hexosaminidase A (alpha polypeptide) (HEXA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HEXA ( NM_000520.6 ) cDNA ORF clone, Homo sapiens(Human) -> NP_000511.2 Homo sapiens hexosaminidase subunit alpha (HEXA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18820
Clone ID Related Accession (Same CDS sequence)	NM_000520.6 , NM_000520.5 , NM_000520.4
Accession Version	NM_000520.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Homo sapiens(human)
Product	beta-hexosaminidase subunit alpha isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC356933.1, BC018927.2, AC009690.17 and BM668409.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_000520.5. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.184818.1, SRR1803614.132876.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACAAGCT CCAGGCTTTG GTTTTCGCTG CTGCTGGCGG CAGCGTTCGC AGGACGGGCG 
ACGGCCCTCT GGCCCTGGCC TCAGAACTTC CAAACCTCCG ACCAGCGCTA CGTCCTTTAC 
CCGAACAACT TTCAATTCCA GTACGATGTC AGCTCGGCCG CGCAGCCCGG CTGCTCAGTC 
CTCGACGAGG CCTTCCAGCG CTATCGTGAC CTGCTTTTCG GTTCCGGGTC TTGGCCCCGT 
CCTTACCTCA CAGGGAAACG GCATACACTG GAGAAGAATG TGTTGGTTGT CTCTGTAGTC 
ACACCTGGAT GTAACCAGCT TCCTACTTTG GAGTCAGTGG AGAATTATAC CCTGACCATA 
AATGATGACC AGTGTTTACT CCTCTCTGAG ACTGTCTGGG GAGCTCTCCG AGGTCTGGAG 
ACTTTTAGCC AGCTTGTTTG GAAATCTGCT GAGGGCACAT TCTTTATCAA CAAGACTGAG 
ATTGAGGACT TTCCCCGCTT TCCTCACCGG GGCTTGCTGT TGGATACATC TCGCCATTAC 
CTGCCACTCT CTAGCATCCT GGACACTCTG GATGTCATGG CGTACAATAA ATTGAACGTG 
TTCCACTGGC ATCTGGTAGA TGATCCTTCC TTCCCATATG AGAGCTTCAC TTTTCCAGAG 
CTCATGAGAA AGGGGTCCTA CAACCCTGTC ACCCACATCT ACACAGCACA GGATGTGAAG 
GAGGTCATTG AATACGCACG GCTCCGGGGT ATCCGTGTGC TTGCAGAGTT TGACACTCCT 
GGCCACACTT TGTCCTGGGG ACCAGGTATC CCTGGATTAC TGACTCCTTG CTACTCTGGG 
TCTGAGCCCT CTGGCACCTT TGGACCAGTG AATCCCAGTC TCAATAATAC CTATGAGTTC 
ATGAGCACAT TCTTCTTAGA AGTCAGCTCT GTCTTCCCAG ATTTTTATCT TCATCTTGGA 
GGAGATGAGG TTGATTTCAC CTGCTGGAAG TCCAACCCAG AGATCCAGGA CTTTATGAGG 
AAGAAAGGCT TCGGTGAGGA CTTCAAGCAG CTGGAGTCCT TCTACATCCA GACGCTGCTG 
GACATCGTCT CTTCTTATGG CAAGGGCTAT GTGGTGTGGC AGGAGGTGTT TGATAATAAA 
GTAAAGATTC AGCCAGACAC AATCATACAG GTGTGGCGAG AGGATATTCC AGTGAACTAT 
ATGAAGGAGC TGGAACTGGT CACCAAGGCC GGCTTCCGGG CCCTTCTCTC TGCCCCCTGG 
TACCTGAACC GTATATCCTA TGGCCCTGAC TGGAAGGATT TCTACATAGT GGAACCCCTG 
GCATTTGAAG GTACCCCTGA GCAGAAGGCT CTGGTGATTG GTGGAGAGGC TTGTATGTGG 
GGAGAATATG TGGACAACAC AAACCTGGTC CCCAGGCTCT GGCCCAGAGC AGGGGCTGTT 
GCCGAAAGGC TGTGGAGCAA CAAGTTGACA TCTGACCTGA CATTTGCCTA TGAACGTTTG 
TCACACTTCC GCTGTGAATT GCTGAGGCGA GGTGTCCAGG CCCAACCCCT CAATGTAGGC 
TTCTGTGAGC AGGAGTTTGA ACAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000511.2
CDS	43..1632
Translation	MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNFQFQYDVSSAAQPGCSVLDEAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDDQCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTEIEDFPRFPHRGLLLDTSRHYLPLSSILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEYARLRGIRVLAEFDTPGHTLSWGPGIPGLLTPCYSGSEPSGTFGPVNPSLNNTYEFMSTFFLEVSSVFPDFYLHLGGDEVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYGKGYVVWQEVFDNKVKIQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYGPDWKDFYIVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKLTSDLTFAYERLSHFRCELLRRGVQAQPLNVGFCEQEFEQT

Target ORF information:

RefSeq Version	NM_000520.6
Organism	Homo sapiens(human)
Definition	Homo sapiens hexosaminidase subunit alpha (HEXA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000520.6

ORF Insert Sequence:

ATGACAAGCT CCAGGCTTTG GTTTTCGCTG CTGCTGGCGG CAGCGTTCGC AGGACGGGCG 
ACGGCCCTCT GGCCCTGGCC TCAGAACTTC CAAACCTCCG ACCAGCGCTA CGTCCTTTAC 
CCGAACAACT TTCAATTCCA GTACGATGTC AGCTCGGCCG CGCAGCCCGG CTGCTCAGTC 
CTCGACGAGG CCTTCCAGCG CTATCGTGAC CTGCTTTTCG GTTCCGGGTC TTGGCCCCGT 
CCTTACCTCA CAGGGAAACG GCATACACTG GAGAAGAATG TGTTGGTTGT CTCTGTAGTC 
ACACCTGGAT GTAACCAGCT TCCTACTTTG GAGTCAGTGG AGAATTATAC CCTGACCATA 
AATGATGACC AGTGTTTACT CCTCTCTGAG ACTGTCTGGG GAGCTCTCCG AGGTCTGGAG 
ACTTTTAGCC AGCTTGTTTG GAAATCTGCT GAGGGCACAT TCTTTATCAA CAAGACTGAG 
ATTGAGGACT TTCCCCGCTT TCCTCACCGG GGCTTGCTGT TGGATACATC TCGCCATTAC 
CTGCCACTCT CTAGCATCCT GGACACTCTG GATGTCATGG CGTACAATAA ATTGAACGTG 
TTCCACTGGC ATCTGGTAGA TGATCCTTCC TTCCCATATG AGAGCTTCAC TTTTCCAGAG 
CTCATGAGAA AGGGGTCCTA CAACCCTGTC ACCCACATCT ACACAGCACA GGATGTGAAG 
GAGGTCATTG AATACGCACG GCTCCGGGGT ATCCGTGTGC TTGCAGAGTT TGACACTCCT 
GGCCACACTT TGTCCTGGGG ACCAGGTATC CCTGGATTAC TGACTCCTTG CTACTCTGGG 
TCTGAGCCCT CTGGCACCTT TGGACCAGTG AATCCCAGTC TCAATAATAC CTATGAGTTC 
ATGAGCACAT TCTTCTTAGA AGTCAGCTCT GTCTTCCCAG ATTTTTATCT TCATCTTGGA 
GGAGATGAGG TTGATTTCAC CTGCTGGAAG TCCAACCCAG AGATCCAGGA CTTTATGAGG 
AAGAAAGGCT TCGGTGAGGA CTTCAAGCAG CTGGAGTCCT TCTACATCCA GACGCTGCTG 
GACATCGTCT CTTCTTATGG CAAGGGCTAT GTGGTGTGGC AGGAGGTGTT TGATAATAAA 
GTAAAGATTC AGCCAGACAC AATCATACAG GTGTGGCGAG AGGATATTCC AGTGAACTAT 
ATGAAGGAGC TGGAACTGGT CACCAAGGCC GGCTTCCGGG CCCTTCTCTC TGCCCCCTGG 
TACCTGAACC GTATATCCTA TGGCCCTGAC TGGAAGGATT TCTACATAGT GGAACCCCTG 
GCATTTGAAG GTACCCCTGA GCAGAAGGCT CTGGTGATTG GTGGAGAGGC TTGTATGTGG 
GGAGAATATG TGGACAACAC AAACCTGGTC CCCAGGCTCT GGCCCAGAGC AGGGGCTGTT 
GCCGAAAGGC TGTGGAGCAA CAAGTTGACA TCTGACCTGA CATTTGCCTA TGAACGTTTG 
TCACACTTCC GCTGTGAATT GCTGAGGCGA GGTGTCCAGG CCCAACCCCT CAATGTAGGC 
TTCTGTGAGC AGGAGTTTGA ACAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEXA ( NM_000520.5 ) cDNA ORF clone, Homo sapiens(Human) -> NP_000511.2 Homo sapiens hexosaminidase subunit alpha (HEXA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18820
Clone ID Related Accession (Same CDS sequence)	NM_000520.6 , NM_000520.5 , NM_000520.4
Accession Version	NM_000520.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	beta-hexosaminidase subunit alpha isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR627386.1, AC009690.17, DC356933.1, BC018927.2 and BM668409.1. This sequence is a reference standard in the RefSeqGene project. On Jan 14, 2016 this sequence version replaced NM_000520.4. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR627386.1, SRR1803617.271482.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAAGCT CCAGGCTTTG GTTTTCGCTG CTGCTGGCGG CAGCGTTCGC AGGACGGGCG 
ACGGCCCTCT GGCCCTGGCC TCAGAACTTC CAAACCTCCG ACCAGCGCTA CGTCCTTTAC 
CCGAACAACT TTCAATTCCA GTACGATGTC AGCTCGGCCG CGCAGCCCGG CTGCTCAGTC 
CTCGACGAGG CCTTCCAGCG CTATCGTGAC CTGCTTTTCG GTTCCGGGTC TTGGCCCCGT 
CCTTACCTCA CAGGGAAACG GCATACACTG GAGAAGAATG TGTTGGTTGT CTCTGTAGTC 
ACACCTGGAT GTAACCAGCT TCCTACTTTG GAGTCAGTGG AGAATTATAC CCTGACCATA 
AATGATGACC AGTGTTTACT CCTCTCTGAG ACTGTCTGGG GAGCTCTCCG AGGTCTGGAG 
ACTTTTAGCC AGCTTGTTTG GAAATCTGCT GAGGGCACAT TCTTTATCAA CAAGACTGAG 
ATTGAGGACT TTCCCCGCTT TCCTCACCGG GGCTTGCTGT TGGATACATC TCGCCATTAC 
CTGCCACTCT CTAGCATCCT GGACACTCTG GATGTCATGG CGTACAATAA ATTGAACGTG 
TTCCACTGGC ATCTGGTAGA TGATCCTTCC TTCCCATATG AGAGCTTCAC TTTTCCAGAG 
CTCATGAGAA AGGGGTCCTA CAACCCTGTC ACCCACATCT ACACAGCACA GGATGTGAAG 
GAGGTCATTG AATACGCACG GCTCCGGGGT ATCCGTGTGC TTGCAGAGTT TGACACTCCT 
GGCCACACTT TGTCCTGGGG ACCAGGTATC CCTGGATTAC TGACTCCTTG CTACTCTGGG 
TCTGAGCCCT CTGGCACCTT TGGACCAGTG AATCCCAGTC TCAATAATAC CTATGAGTTC 
ATGAGCACAT TCTTCTTAGA AGTCAGCTCT GTCTTCCCAG ATTTTTATCT TCATCTTGGA 
GGAGATGAGG TTGATTTCAC CTGCTGGAAG TCCAACCCAG AGATCCAGGA CTTTATGAGG 
AAGAAAGGCT TCGGTGAGGA CTTCAAGCAG CTGGAGTCCT TCTACATCCA GACGCTGCTG 
GACATCGTCT CTTCTTATGG CAAGGGCTAT GTGGTGTGGC AGGAGGTGTT TGATAATAAA 
GTAAAGATTC AGCCAGACAC AATCATACAG GTGTGGCGAG AGGATATTCC AGTGAACTAT 
ATGAAGGAGC TGGAACTGGT CACCAAGGCC GGCTTCCGGG CCCTTCTCTC TGCCCCCTGG 
TACCTGAACC GTATATCCTA TGGCCCTGAC TGGAAGGATT TCTACATAGT GGAACCCCTG 
GCATTTGAAG GTACCCCTGA GCAGAAGGCT CTGGTGATTG GTGGAGAGGC TTGTATGTGG 
GGAGAATATG TGGACAACAC AAACCTGGTC CCCAGGCTCT GGCCCAGAGC AGGGGCTGTT 
GCCGAAAGGC TGTGGAGCAA CAAGTTGACA TCTGACCTGA CATTTGCCTA TGAACGTTTG 
TCACACTTCC GCTGTGAATT GCTGAGGCGA GGTGTCCAGG CCCAACCCCT CAATGTAGGC 
TTCTGTGAGC AGGAGTTTGA ACAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000511.2
CDS	502..2091
Translation	MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNFQFQYDVSSAAQPGCSVLDEAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDDQCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTEIEDFPRFPHRGLLLDTSRHYLPLSSILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEYARLRGIRVLAEFDTPGHTLSWGPGIPGLLTPCYSGSEPSGTFGPVNPSLNNTYEFMSTFFLEVSSVFPDFYLHLGGDEVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYGKGYVVWQEVFDNKVKIQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYGPDWKDFYIVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKLTSDLTFAYERLSHFRCELLRRGVQAQPLNVGFCEQEFEQT

Target ORF information:

RefSeq Version	NM_000520.5
Organism	Homo sapiens(human)
Definition	Homo sapiens hexosaminidase subunit alpha (HEXA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000520.5

ORF Insert Sequence:

ATGACAAGCT CCAGGCTTTG GTTTTCGCTG CTGCTGGCGG CAGCGTTCGC AGGACGGGCG 
ACGGCCCTCT GGCCCTGGCC TCAGAACTTC CAAACCTCCG ACCAGCGCTA CGTCCTTTAC 
CCGAACAACT TTCAATTCCA GTACGATGTC AGCTCGGCCG CGCAGCCCGG CTGCTCAGTC 
CTCGACGAGG CCTTCCAGCG CTATCGTGAC CTGCTTTTCG GTTCCGGGTC TTGGCCCCGT 
CCTTACCTCA CAGGGAAACG GCATACACTG GAGAAGAATG TGTTGGTTGT CTCTGTAGTC 
ACACCTGGAT GTAACCAGCT TCCTACTTTG GAGTCAGTGG AGAATTATAC CCTGACCATA 
AATGATGACC AGTGTTTACT CCTCTCTGAG ACTGTCTGGG GAGCTCTCCG AGGTCTGGAG 
ACTTTTAGCC AGCTTGTTTG GAAATCTGCT GAGGGCACAT TCTTTATCAA CAAGACTGAG 
ATTGAGGACT TTCCCCGCTT TCCTCACCGG GGCTTGCTGT TGGATACATC TCGCCATTAC 
CTGCCACTCT CTAGCATCCT GGACACTCTG GATGTCATGG CGTACAATAA ATTGAACGTG 
TTCCACTGGC ATCTGGTAGA TGATCCTTCC TTCCCATATG AGAGCTTCAC TTTTCCAGAG 
CTCATGAGAA AGGGGTCCTA CAACCCTGTC ACCCACATCT ACACAGCACA GGATGTGAAG 
GAGGTCATTG AATACGCACG GCTCCGGGGT ATCCGTGTGC TTGCAGAGTT TGACACTCCT 
GGCCACACTT TGTCCTGGGG ACCAGGTATC CCTGGATTAC TGACTCCTTG CTACTCTGGG 
TCTGAGCCCT CTGGCACCTT TGGACCAGTG AATCCCAGTC TCAATAATAC CTATGAGTTC 
ATGAGCACAT TCTTCTTAGA AGTCAGCTCT GTCTTCCCAG ATTTTTATCT TCATCTTGGA 
GGAGATGAGG TTGATTTCAC CTGCTGGAAG TCCAACCCAG AGATCCAGGA CTTTATGAGG 
AAGAAAGGCT TCGGTGAGGA CTTCAAGCAG CTGGAGTCCT TCTACATCCA GACGCTGCTG 
GACATCGTCT CTTCTTATGG CAAGGGCTAT GTGGTGTGGC AGGAGGTGTT TGATAATAAA 
GTAAAGATTC AGCCAGACAC AATCATACAG GTGTGGCGAG AGGATATTCC AGTGAACTAT 
ATGAAGGAGC TGGAACTGGT CACCAAGGCC GGCTTCCGGG CCCTTCTCTC TGCCCCCTGG 
TACCTGAACC GTATATCCTA TGGCCCTGAC TGGAAGGATT TCTACATAGT GGAACCCCTG 
GCATTTGAAG GTACCCCTGA GCAGAAGGCT CTGGTGATTG GTGGAGAGGC TTGTATGTGG 
GGAGAATATG TGGACAACAC AAACCTGGTC CCCAGGCTCT GGCCCAGAGC AGGGGCTGTT 
GCCGAAAGGC TGTGGAGCAA CAAGTTGACA TCTGACCTGA CATTTGCCTA TGAACGTTTG 
TCACACTTCC GCTGTGAATT GCTGAGGCGA GGTGTCCAGG CCCAACCCCT CAATGTAGGC 
TTCTGTGAGC AGGAGTTTGA ACAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEXA ( NM_000520.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_000511.2 Homo sapiens hexosaminidase A (alpha polypeptide) (HEXA), mRNA.

Price & Availability↑

CloneID	OHu18820
Clone ID Related Accession (Same CDS sequence)	NM_000520.6 , NM_000520.5 , NM_000520.4
Accession Version	NM_000520.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	beta-hexosaminidase subunit alpha preproprotein
Comment

ATGACAAGCT CCAGGCTTTG GTTTTCGCTG CTGCTGGCGG CAGCGTTCGC AGGACGGGCG 
ACGGCCCTCT GGCCCTGGCC TCAGAACTTC CAAACCTCCG ACCAGCGCTA CGTCCTTTAC 
CCGAACAACT TTCAATTCCA GTACGATGTC AGCTCGGCCG CGCAGCCCGG CTGCTCAGTC 
CTCGACGAGG CCTTCCAGCG CTATCGTGAC CTGCTTTTCG GTTCCGGGTC TTGGCCCCGT 
CCTTACCTCA CAGGGAAACG GCATACACTG GAGAAGAATG TGTTGGTTGT CTCTGTAGTC 
ACACCTGGAT GTAACCAGCT TCCTACTTTG GAGTCAGTGG AGAATTATAC CCTGACCATA 
AATGATGACC AGTGTTTACT CCTCTCTGAG ACTGTCTGGG GAGCTCTCCG AGGTCTGGAG 
ACTTTTAGCC AGCTTGTTTG GAAATCTGCT GAGGGCACAT TCTTTATCAA CAAGACTGAG 
ATTGAGGACT TTCCCCGCTT TCCTCACCGG GGCTTGCTGT TGGATACATC TCGCCATTAC 
CTGCCACTCT CTAGCATCCT GGACACTCTG GATGTCATGG CGTACAATAA ATTGAACGTG 
TTCCACTGGC ATCTGGTAGA TGATCCTTCC TTCCCATATG AGAGCTTCAC TTTTCCAGAG 
CTCATGAGAA AGGGGTCCTA CAACCCTGTC ACCCACATCT ACACAGCACA GGATGTGAAG 
GAGGTCATTG AATACGCACG GCTCCGGGGT ATCCGTGTGC TTGCAGAGTT TGACACTCCT 
GGCCACACTT TGTCCTGGGG ACCAGGTATC CCTGGATTAC TGACTCCTTG CTACTCTGGG 
TCTGAGCCCT CTGGCACCTT TGGACCAGTG AATCCCAGTC TCAATAATAC CTATGAGTTC 
ATGAGCACAT TCTTCTTAGA AGTCAGCTCT GTCTTCCCAG ATTTTTATCT TCATCTTGGA 
GGAGATGAGG TTGATTTCAC CTGCTGGAAG TCCAACCCAG AGATCCAGGA CTTTATGAGG 
AAGAAAGGCT TCGGTGAGGA CTTCAAGCAG CTGGAGTCCT TCTACATCCA GACGCTGCTG 
GACATCGTCT CTTCTTATGG CAAGGGCTAT GTGGTGTGGC AGGAGGTGTT TGATAATAAA 
GTAAAGATTC AGCCAGACAC AATCATACAG GTGTGGCGAG AGGATATTCC AGTGAACTAT 
ATGAAGGAGC TGGAACTGGT CACCAAGGCC GGCTTCCGGG CCCTTCTCTC TGCCCCCTGG 
TACCTGAACC GTATATCCTA TGGCCCTGAC TGGAAGGATT TCTACATAGT GGAACCCCTG 
GCATTTGAAG GTACCCCTGA GCAGAAGGCT CTGGTGATTG GTGGAGAGGC TTGTATGTGG 
GGAGAATATG TGGACAACAC AAACCTGGTC CCCAGGCTCT GGCCCAGAGC AGGGGCTGTT 
GCCGAAAGGC TGTGGAGCAA CAAGTTGACA TCTGACCTGA CATTTGCCTA TGAACGTTTG 
TCACACTTCC GCTGTGAATT GCTGAGGCGA GGTGTCCAGG CCCAACCCCT CAATGTAGGC 
TTCTGTGAGC AGGAGTTTGA ACAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_000511.2

Translation

MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNFQFQYDVSSAAQPGCSVLDEAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDDQCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTEIEDFPRFPHRGLLLDTSRHYLPLSSILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEYARLRGIRVLAEFDTPGHTLSWGPGIPGLLTPCYSGSEPSGTFGPVNPSLNNTYEFMSTFFLEVSSVFPDFYLHLGGDEVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYGKGYVVWQEVFDNKVKIQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYGPDWKDFYIVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKLTSDLTFAYERLSHFRCELLRRGVQAQPLNVGFCEQEFEQT

Target ORF information:

RefSeq Version	NM_000520.4
Organism	Homo sapiens(Human)
Definition	Homo sapiens hexosaminidase A (alpha polypeptide) (HEXA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000520.4

ORF Insert Sequence:

ATGACAAGCT CCAGGCTTTG GTTTTCGCTG CTGCTGGCGG CAGCGTTCGC AGGACGGGCG 
ACGGCCCTCT GGCCCTGGCC TCAGAACTTC CAAACCTCCG ACCAGCGCTA CGTCCTTTAC 
CCGAACAACT TTCAATTCCA GTACGATGTC AGCTCGGCCG CGCAGCCCGG CTGCTCAGTC 
CTCGACGAGG CCTTCCAGCG CTATCGTGAC CTGCTTTTCG GTTCCGGGTC TTGGCCCCGT 
CCTTACCTCA CAGGGAAACG GCATACACTG GAGAAGAATG TGTTGGTTGT CTCTGTAGTC 
ACACCTGGAT GTAACCAGCT TCCTACTTTG GAGTCAGTGG AGAATTATAC CCTGACCATA 
AATGATGACC AGTGTTTACT CCTCTCTGAG ACTGTCTGGG GAGCTCTCCG AGGTCTGGAG 
ACTTTTAGCC AGCTTGTTTG GAAATCTGCT GAGGGCACAT TCTTTATCAA CAAGACTGAG 
ATTGAGGACT TTCCCCGCTT TCCTCACCGG GGCTTGCTGT TGGATACATC TCGCCATTAC 
CTGCCACTCT CTAGCATCCT GGACACTCTG GATGTCATGG CGTACAATAA ATTGAACGTG 
TTCCACTGGC ATCTGGTAGA TGATCCTTCC TTCCCATATG AGAGCTTCAC TTTTCCAGAG 
CTCATGAGAA AGGGGTCCTA CAACCCTGTC ACCCACATCT ACACAGCACA GGATGTGAAG 
GAGGTCATTG AATACGCACG GCTCCGGGGT ATCCGTGTGC TTGCAGAGTT TGACACTCCT 
GGCCACACTT TGTCCTGGGG ACCAGGTATC CCTGGATTAC TGACTCCTTG CTACTCTGGG 
TCTGAGCCCT CTGGCACCTT TGGACCAGTG AATCCCAGTC TCAATAATAC CTATGAGTTC 
ATGAGCACAT TCTTCTTAGA AGTCAGCTCT GTCTTCCCAG ATTTTTATCT TCATCTTGGA 
GGAGATGAGG TTGATTTCAC CTGCTGGAAG TCCAACCCAG AGATCCAGGA CTTTATGAGG 
AAGAAAGGCT TCGGTGAGGA CTTCAAGCAG CTGGAGTCCT TCTACATCCA GACGCTGCTG 
GACATCGTCT CTTCTTATGG CAAGGGCTAT GTGGTGTGGC AGGAGGTGTT TGATAATAAA 
GTAAAGATTC AGCCAGACAC AATCATACAG GTGTGGCGAG AGGATATTCC AGTGAACTAT 
ATGAAGGAGC TGGAACTGGT CACCAAGGCC GGCTTCCGGG CCCTTCTCTC TGCCCCCTGG 
TACCTGAACC GTATATCCTA TGGCCCTGAC TGGAAGGATT TCTACATAGT GGAACCCCTG 
GCATTTGAAG GTACCCCTGA GCAGAAGGCT CTGGTGATTG GTGGAGAGGC TTGTATGTGG 
GGAGAATATG TGGACAACAC AAACCTGGTC CCCAGGCTCT GGCCCAGAGC AGGGGCTGTT 
GCCGAAAGGC TGTGGAGCAA CAAGTTGACA TCTGACCTGA CATTTGCCTA TGAACGTTTG 
TCACACTTCC GCTGTGAATT GCTGAGGCGA GGTGTCCAGG CCCAACCCCT CAATGTAGGC 
TTCTGTGAGC AGGAGTTTGA ACAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEXA ( NM_001318825.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001305754.1 Homo sapiens hexosaminidase subunit alpha (HEXA), transcript variant 1, mRNA.

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CloneID	OHu100681
Clone ID Related Accession (Same CDS sequence)	NM_001318825.2 , NM_001318825.1
Accession Version	NM_001318825.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	beta-hexosaminidase subunit alpha isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC356933.1, AK301000.1, AC009690.17, M13520.1 and BM668409.1. On Jun 2, 2019 this sequence version replaced NM_001318825.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). The encoded isoform (1) may undergo proteolytic processing similar to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.50410.1, SRR1803616.139283.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAAGCT CCAGGCTTTG GTTTTCGCTG CTGCTGGCGG CAGCGTTCGC AGGACGGGCG 
ACGGCCCTCT GGCCCTGGCC TCAGAACTTC CAAACCTCCG ACCAGCGCTA CGTCCTTTAC 
CCGAACAACT TTCAATTCCA GTACGATGTC AGCTCGGCCG CGCAGCCCGG CTGCTCAGTC 
CTCGACGAGG CCTTCCAGCG CTATCGTGAC CTGCTTTTCG GTTCCGGGTC TTGGCCCCGT 
CCTTACCTCA CAGGTTGGCC GCACCAAGCT TACCCTGTCT TCCTAGGGAA ACGGCATACA 
CTGGAGAAGA ATGTGTTGGT TGTCTCTGTA GTCACACCTG GATGTAACCA GCTTCCTACT 
TTGGAGTCAG TGGAGAATTA TACCCTGACC ATAAATGATG ACCAGTGTTT ACTCCTCTCT 
GAGACTGTCT GGGGAGCTCT CCGAGGTCTG GAGACTTTTA GCCAGCTTGT TTGGAAATCT 
GCTGAGGGCA CATTCTTTAT CAACAAGACT GAGATTGAGG ACTTTCCCCG CTTTCCTCAC 
CGGGGCTTGC TGTTGGATAC ATCTCGCCAT TACCTGCCAC TCTCTAGCAT CCTGGACACT 
CTGGATGTCA TGGCGTACAA TAAATTGAAC GTGTTCCACT GGCATCTGGT AGATGATCCT 
TCCTTCCCAT ATGAGAGCTT CACTTTTCCA GAGCTCATGA GAAAGGGGTC CTACAACCCT 
GTCACCCACA TCTACACAGC ACAGGATGTG AAGGAGGTCA TTGAATACGC ACGGCTCCGG 
GGTATCCGTG TGCTTGCAGA GTTTGACACT CCTGGCCACA CTTTGTCCTG GGGACCAGGT 
ATCCCTGGAT TACTGACTCC TTGCTACTCT GGGTCTGAGC CCTCTGGCAC CTTTGGACCA 
GTGAATCCCA GTCTCAATAA TACCTATGAG TTCATGAGCA CATTCTTCTT AGAAGTCAGC 
TCTGTCTTCC CAGATTTTTA TCTTCATCTT GGAGGAGATG AGGTTGATTT CACCTGCTGG 
AAGTCCAACC CAGAGATCCA GGACTTTATG AGGAAGAAAG GCTTCGGTGA GGACTTCAAG 
CAGCTGGAGT CCTTCTACAT CCAGACGCTG CTGGACATCG TCTCTTCTTA TGGCAAGGGC 
TATGTGGTGT GGCAGGAGGT GTTTGATAAT AAAGTAAAGA TTCAGCCAGA CACAATCATA 
CAGGTGTGGC GAGAGGATAT TCCAGTGAAC TATATGAAGG AGCTGGAACT GGTCACCAAG 
GCCGGCTTCC GGGCCCTTCT CTCTGCCCCC TGGTACCTGA ACCGTATATC CTATGGCCCT 
GACTGGAAGG ATTTCTACAT AGTGGAACCC CTGGCATTTG AAGGTACCCC TGAGCAGAAG 
GCTCTGGTGA TTGGTGGAGA GGCTTGTATG TGGGGAGAAT ATGTGGACAA CACAAACCTG 
GTCCCCAGGC TCTGGCCCAG AGCAGGGGCT GTTGCCGAAA GGCTGTGGAG CAACAAGTTG 
ACATCTGACC TGACATTTGC CTATGAACGT TTGTCACACT TCCGCTGTGA ATTGCTGAGG 
CGAGGTGTCC AGGCCCAACC CCTCAATGTA GGCTTCTGTG AGCAGGAGTT TGAACAGACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305754.1
CDS	43..1665
Translation	MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNFQFQYDVSSAAQPGCSVLDEAFQRYRDLLFGSGSWPRPYLTGWPHQAYPVFLGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDDQCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTEIEDFPRFPHRGLLLDTSRHYLPLSSILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEYARLRGIRVLAEFDTPGHTLSWGPGIPGLLTPCYSGSEPSGTFGPVNPSLNNTYEFMSTFFLEVSSVFPDFYLHLGGDEVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYGKGYVVWQEVFDNKVKIQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYGPDWKDFYIVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKLTSDLTFAYERLSHFRCELLRRGVQAQPLNVGFCEQEFEQT

Target ORF information:

RefSeq Version	NM_001318825.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hexosaminidase subunit alpha (HEXA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318825.2

ORF Insert Sequence:

ATGACAAGCT CCAGGCTTTG GTTTTCGCTG CTGCTGGCGG CAGCGTTCGC AGGACGGGCG 
ACGGCCCTCT GGCCCTGGCC TCAGAACTTC CAAACCTCCG ACCAGCGCTA CGTCCTTTAC 
CCGAACAACT TTCAATTCCA GTACGATGTC AGCTCGGCCG CGCAGCCCGG CTGCTCAGTC 
CTCGACGAGG CCTTCCAGCG CTATCGTGAC CTGCTTTTCG GTTCCGGGTC TTGGCCCCGT 
CCTTACCTCA CAGGTTGGCC GCACCAAGCT TACCCTGTCT TCCTAGGGAA ACGGCATACA 
CTGGAGAAGA ATGTGTTGGT TGTCTCTGTA GTCACACCTG GATGTAACCA GCTTCCTACT 
TTGGAGTCAG TGGAGAATTA TACCCTGACC ATAAATGATG ACCAGTGTTT ACTCCTCTCT 
GAGACTGTCT GGGGAGCTCT CCGAGGTCTG GAGACTTTTA GCCAGCTTGT TTGGAAATCT 
GCTGAGGGCA CATTCTTTAT CAACAAGACT GAGATTGAGG ACTTTCCCCG CTTTCCTCAC 
CGGGGCTTGC TGTTGGATAC ATCTCGCCAT TACCTGCCAC TCTCTAGCAT CCTGGACACT 
CTGGATGTCA TGGCGTACAA TAAATTGAAC GTGTTCCACT GGCATCTGGT AGATGATCCT 
TCCTTCCCAT ATGAGAGCTT CACTTTTCCA GAGCTCATGA GAAAGGGGTC CTACAACCCT 
GTCACCCACA TCTACACAGC ACAGGATGTG AAGGAGGTCA TTGAATACGC ACGGCTCCGG 
GGTATCCGTG TGCTTGCAGA GTTTGACACT CCTGGCCACA CTTTGTCCTG GGGACCAGGT 
ATCCCTGGAT TACTGACTCC TTGCTACTCT GGGTCTGAGC CCTCTGGCAC CTTTGGACCA 
GTGAATCCCA GTCTCAATAA TACCTATGAG TTCATGAGCA CATTCTTCTT AGAAGTCAGC 
TCTGTCTTCC CAGATTTTTA TCTTCATCTT GGAGGAGATG AGGTTGATTT CACCTGCTGG 
AAGTCCAACC CAGAGATCCA GGACTTTATG AGGAAGAAAG GCTTCGGTGA GGACTTCAAG 
CAGCTGGAGT CCTTCTACAT CCAGACGCTG CTGGACATCG TCTCTTCTTA TGGCAAGGGC 
TATGTGGTGT GGCAGGAGGT GTTTGATAAT AAAGTAAAGA TTCAGCCAGA CACAATCATA 
CAGGTGTGGC GAGAGGATAT TCCAGTGAAC TATATGAAGG AGCTGGAACT GGTCACCAAG 
GCCGGCTTCC GGGCCCTTCT CTCTGCCCCC TGGTACCTGA ACCGTATATC CTATGGCCCT 
GACTGGAAGG ATTTCTACAT AGTGGAACCC CTGGCATTTG AAGGTACCCC TGAGCAGAAG 
GCTCTGGTGA TTGGTGGAGA GGCTTGTATG TGGGGAGAAT ATGTGGACAA CACAAACCTG 
GTCCCCAGGC TCTGGCCCAG AGCAGGGGCT GTTGCCGAAA GGCTGTGGAG CAACAAGTTG 
ACATCTGACC TGACATTTGC CTATGAACGT TTGTCACACT TCCGCTGTGA ATTGCTGAGG 
CGAGGTGTCC AGGCCCAACC CCTCAATGTA GGCTTCTGTG AGCAGGAGTT TGAACAGACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEXA ( NM_001318825.1 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001305754.1 Homo sapiens hexosaminidase subunit alpha (HEXA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu100681
Clone ID Related Accession (Same CDS sequence)	NM_001318825.2 , NM_001318825.1
Accession Version	NM_001318825.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	beta-hexosaminidase subunit alpha isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR627386.1, AC009690.17, DC356933.1, AK301000.1, M13520.1 and BM668409.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). The encoded isoform (1) may undergo proteolytic processing similar to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.139283.1, SRR1660805.50410.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAAGCT CCAGGCTTTG GTTTTCGCTG CTGCTGGCGG CAGCGTTCGC AGGACGGGCG 
ACGGCCCTCT GGCCCTGGCC TCAGAACTTC CAAACCTCCG ACCAGCGCTA CGTCCTTTAC 
CCGAACAACT TTCAATTCCA GTACGATGTC AGCTCGGCCG CGCAGCCCGG CTGCTCAGTC 
CTCGACGAGG CCTTCCAGCG CTATCGTGAC CTGCTTTTCG GTTCCGGGTC TTGGCCCCGT 
CCTTACCTCA CAGGTTGGCC GCACCAAGCT TACCCTGTCT TCCTAGGGAA ACGGCATACA 
CTGGAGAAGA ATGTGTTGGT TGTCTCTGTA GTCACACCTG GATGTAACCA GCTTCCTACT 
TTGGAGTCAG TGGAGAATTA TACCCTGACC ATAAATGATG ACCAGTGTTT ACTCCTCTCT 
GAGACTGTCT GGGGAGCTCT CCGAGGTCTG GAGACTTTTA GCCAGCTTGT TTGGAAATCT 
GCTGAGGGCA CATTCTTTAT CAACAAGACT GAGATTGAGG ACTTTCCCCG CTTTCCTCAC 
CGGGGCTTGC TGTTGGATAC ATCTCGCCAT TACCTGCCAC TCTCTAGCAT CCTGGACACT 
CTGGATGTCA TGGCGTACAA TAAATTGAAC GTGTTCCACT GGCATCTGGT AGATGATCCT 
TCCTTCCCAT ATGAGAGCTT CACTTTTCCA GAGCTCATGA GAAAGGGGTC CTACAACCCT 
GTCACCCACA TCTACACAGC ACAGGATGTG AAGGAGGTCA TTGAATACGC ACGGCTCCGG 
GGTATCCGTG TGCTTGCAGA GTTTGACACT CCTGGCCACA CTTTGTCCTG GGGACCAGGT 
ATCCCTGGAT TACTGACTCC TTGCTACTCT GGGTCTGAGC CCTCTGGCAC CTTTGGACCA 
GTGAATCCCA GTCTCAATAA TACCTATGAG TTCATGAGCA CATTCTTCTT AGAAGTCAGC 
TCTGTCTTCC CAGATTTTTA TCTTCATCTT GGAGGAGATG AGGTTGATTT CACCTGCTGG 
AAGTCCAACC CAGAGATCCA GGACTTTATG AGGAAGAAAG GCTTCGGTGA GGACTTCAAG 
CAGCTGGAGT CCTTCTACAT CCAGACGCTG CTGGACATCG TCTCTTCTTA TGGCAAGGGC 
TATGTGGTGT GGCAGGAGGT GTTTGATAAT AAAGTAAAGA TTCAGCCAGA CACAATCATA 
CAGGTGTGGC GAGAGGATAT TCCAGTGAAC TATATGAAGG AGCTGGAACT GGTCACCAAG 
GCCGGCTTCC GGGCCCTTCT CTCTGCCCCC TGGTACCTGA ACCGTATATC CTATGGCCCT 
GACTGGAAGG ATTTCTACAT AGTGGAACCC CTGGCATTTG AAGGTACCCC TGAGCAGAAG 
GCTCTGGTGA TTGGTGGAGA GGCTTGTATG TGGGGAGAAT ATGTGGACAA CACAAACCTG 
GTCCCCAGGC TCTGGCCCAG AGCAGGGGCT GTTGCCGAAA GGCTGTGGAG CAACAAGTTG 
ACATCTGACC TGACATTTGC CTATGAACGT TTGTCACACT TCCGCTGTGA ATTGCTGAGG 
CGAGGTGTCC AGGCCCAACC CCTCAATGTA GGCTTCTGTG AGCAGGAGTT TGAACAGACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305754.1
CDS	502..2124
Translation	MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNFQFQYDVSSAAQPGCSVLDEAFQRYRDLLFGSGSWPRPYLTGWPHQAYPVFLGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDDQCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTEIEDFPRFPHRGLLLDTSRHYLPLSSILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEYARLRGIRVLAEFDTPGHTLSWGPGIPGLLTPCYSGSEPSGTFGPVNPSLNNTYEFMSTFFLEVSSVFPDFYLHLGGDEVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYGKGYVVWQEVFDNKVKIQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYGPDWKDFYIVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKLTSDLTFAYERLSHFRCELLRRGVQAQPLNVGFCEQEFEQT

Target ORF information:

RefSeq Version	NM_001318825.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hexosaminidase subunit alpha (HEXA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318825.1

ORF Insert Sequence:

ATGACAAGCT CCAGGCTTTG GTTTTCGCTG CTGCTGGCGG CAGCGTTCGC AGGACGGGCG 
ACGGCCCTCT GGCCCTGGCC TCAGAACTTC CAAACCTCCG ACCAGCGCTA CGTCCTTTAC 
CCGAACAACT TTCAATTCCA GTACGATGTC AGCTCGGCCG CGCAGCCCGG CTGCTCAGTC 
CTCGACGAGG CCTTCCAGCG CTATCGTGAC CTGCTTTTCG GTTCCGGGTC TTGGCCCCGT 
CCTTACCTCA CAGGTTGGCC GCACCAAGCT TACCCTGTCT TCCTAGGGAA ACGGCATACA 
CTGGAGAAGA ATGTGTTGGT TGTCTCTGTA GTCACACCTG GATGTAACCA GCTTCCTACT 
TTGGAGTCAG TGGAGAATTA TACCCTGACC ATAAATGATG ACCAGTGTTT ACTCCTCTCT 
GAGACTGTCT GGGGAGCTCT CCGAGGTCTG GAGACTTTTA GCCAGCTTGT TTGGAAATCT 
GCTGAGGGCA CATTCTTTAT CAACAAGACT GAGATTGAGG ACTTTCCCCG CTTTCCTCAC 
CGGGGCTTGC TGTTGGATAC ATCTCGCCAT TACCTGCCAC TCTCTAGCAT CCTGGACACT 
CTGGATGTCA TGGCGTACAA TAAATTGAAC GTGTTCCACT GGCATCTGGT AGATGATCCT 
TCCTTCCCAT ATGAGAGCTT CACTTTTCCA GAGCTCATGA GAAAGGGGTC CTACAACCCT 
GTCACCCACA TCTACACAGC ACAGGATGTG AAGGAGGTCA TTGAATACGC ACGGCTCCGG 
GGTATCCGTG TGCTTGCAGA GTTTGACACT CCTGGCCACA CTTTGTCCTG GGGACCAGGT 
ATCCCTGGAT TACTGACTCC TTGCTACTCT GGGTCTGAGC CCTCTGGCAC CTTTGGACCA 
GTGAATCCCA GTCTCAATAA TACCTATGAG TTCATGAGCA CATTCTTCTT AGAAGTCAGC 
TCTGTCTTCC CAGATTTTTA TCTTCATCTT GGAGGAGATG AGGTTGATTT CACCTGCTGG 
AAGTCCAACC CAGAGATCCA GGACTTTATG AGGAAGAAAG GCTTCGGTGA GGACTTCAAG 
CAGCTGGAGT CCTTCTACAT CCAGACGCTG CTGGACATCG TCTCTTCTTA TGGCAAGGGC 
TATGTGGTGT GGCAGGAGGT GTTTGATAAT AAAGTAAAGA TTCAGCCAGA CACAATCATA 
CAGGTGTGGC GAGAGGATAT TCCAGTGAAC TATATGAAGG AGCTGGAACT GGTCACCAAG 
GCCGGCTTCC GGGCCCTTCT CTCTGCCCCC TGGTACCTGA ACCGTATATC CTATGGCCCT 
GACTGGAAGG ATTTCTACAT AGTGGAACCC CTGGCATTTG AAGGTACCCC TGAGCAGAAG 
GCTCTGGTGA TTGGTGGAGA GGCTTGTATG TGGGGAGAAT ATGTGGACAA CACAAACCTG 
GTCCCCAGGC TCTGGCCCAG AGCAGGGGCT GTTGCCGAAA GGCTGTGGAG CAACAAGTTG 
ACATCTGACC TGACATTTGC CTATGAACGT TTGTCACACT TCCGCTGTGA ATTGCTGAGG 
CGAGGTGTCC AGGCCCAACC CCTCAATGTA GGCTTCTGTG AGCAGGAGTT TGAACAGACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pediatric reference data on activity of urinary N-acetyl-?-D-hexosaminidase and its isoenzymes.
Advances in medical sciences63(1)94-99(2017 Aug)
Zalewska-Szajda B,Taranta-Janusz K,Chojnowska S,Waszkiewicz N,Zwierz K,Wasilewska A

Tay-Sachs disease mutations in HEXA target the ? chain of hexosaminidase A to endoplasmic reticulum-associated degradation.
Molecular biology of the cell27(24)3813-3827(2016 Dec)
Dersh D,Iwamoto Y,Argon Y

Identification of domains in human beta-hexosaminidase that determine substrate specificity.
The Journal of biological chemistry271(29)17377-82(1996 Jul)
Pennybacker M,Liessem B,Moczall H,Tifft CJ,Sandhoff K,Proia RL

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HEXA cDNA ORF clone, Homo sapiens(Human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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