NDUFAF8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDUFAF8
Entrez Gene ID	284184
Full Name	NADH:ubiquinone oxidoreductase complex assembly factor 8
Synonyms	C17orf89
General protein information	Preferred Names NADH:ubiquinone oxidoreductase complex assembly factor 8 Names NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 8
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q25.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001086521.1	NP_001079990.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 8 isoform 2
NM_001353403.1	NP_001340332.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 8 isoform 3
NM_001086521.2	NP_001079990.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 8 isoform 2
NM_001353402.1	NP_001340331.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 8 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	C17orf89	NP_001079990.1
Rattus norvegicus (Norway rat)	LOC690871	NP_001182411.1
Pan troglodytes (chimpanzee)	C17H17orf89	XP_001162283.1
Canis lupus familiaris (dog)	C9H17orf89	XP_003435222.1
Bos taurus (cattle)	C19H17orf89	XP_003587520.1
	LOC100429129	XP_002800684.1
Mus musculus (house mouse)	1810043H04Rik	NP_001103712.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.
Floyd BJ, Wilkerson EM, Veling MT, Minogue CE, Xia C, Beebe ET, Wrobel RL, Cho H, Kremer LS, Alston CL, Gromek KA, Dolan BK, Ulbrich A, Stefely JA, Bohl SL, Werner KM, Jochem A, Westphall MS, Rensvold JW, Taylor RW, Prokisch H, Kim JP, Coon JJ, Pagliarini DJ
Molecular cell63(4)621-632(2016 Aug)

Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry.
Molina H, Horn DM, Tang N, Mathivanan S, Pandey A
Proceedings of the National Academy of Sciences of the United States of America104(7)2199-204(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NDUFAF8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDUFAF8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu103229	NM_001353403.1 Latest version!	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 8 (NDUFAF8), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu103315	NM_001353402.1 Latest version!	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 8 (NDUFAF8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
OHu31177	NM_001086521.2 Latest version!	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 8 (NDUFAF8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
View Old Accession Versions >>
OHu31177	NM_001086521.1	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 8 (NDUFAF8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDUFAF8 ( NM_001353403.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340332.1 Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 8 (NDUFAF8), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu103229
Clone ID Related Accession (Same CDS sequence)	NM_001353403.1
Accession Version	NM_001353403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 8 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC027601.25. Transcript Variant: This variant (3) uses an alternate splice junction compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: ERR279846.1036.1, SRR1163657.233092.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAGATG TGTGCAAAAA AGCGCCTTGT AAAACGCGCC AAGAAGACGC TGGAGGGAGG 
CTGTTAGGAG GGACTCTGAG CTTCACACCT GTCTGCTGCC ATGGGTGCAG AGCCCTAGTC 
CTGATGGCCC CTGGTGGCAC ATATCGAATG CCTAGGGCAG AAAGGAAGTG GGAATGGCGA 
AGATGTGACA TTCCTCGGTG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340332.1
CDS	477..680
Translation	MGDVCKKAPCKTRQEDAGGRLLGGTLSFTPVCCHGCRALVLMAPGGTYRMPRAERKWEWRRCDIPRC

Target ORF information:

RefSeq Version	NM_001353403.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 8 (NDUFAF8), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353403.1

ORF Insert Sequence:

ATGGGAGATG TGTGCAAAAA AGCGCCTTGT AAAACGCGCC AAGAAGACGC TGGAGGGAGG 
CTGTTAGGAG GGACTCTGAG CTTCACACCT GTCTGCTGCC ATGGGTGCAG AGCCCTAGTC 
CTGATGGCCC CTGGTGGCAC ATATCGAATG CCTAGGGCAG AAAGGAAGTG GGAATGGCGA 
AGATGTGACA TTCCTCGGTG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFAF8 ( NM_001353402.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340331.1 Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 8 (NDUFAF8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu103315
Clone ID Related Accession (Same CDS sequence)	NM_001353402.1
Accession Version	NM_001353402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 8 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC027601.25. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: HY116350.1, HY090874.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGCTA ACGGAGCGGT GTGGGGCCGC GTGCGAAGCC GCCTCCGCGC CTTCCCCGAG 
CGGCTGGCCG CCTGCGGGGC CGAGGCCGCG GCGTACGGCA GGTGCGTGCA GGCCTCCACG 
GCCCCGGGCG GCCGCCTGAG TAAGGACTTC TGCGCGCGGG AGTTCGAGGC CCTGCGGAGC 
TGCTTCGCCG CTGCGGTAGG CCAAGAAGAC GCTGGAGGGA GGCTGTTAGG AGGGACTCTG 
AGCTTCACAC CTGTCTGCTG CCATGGGTGC AGAGCCCTAG TCCTGATGGC CCCTGGTGGC 
ACATATCGAA TGCCTAGGGC AGAAAGGAAG TGGGAATGGC GAAGATGTGA CATTCCTCGG 
TGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340331.1
CDS	54..419
Translation	MSANGAVWGRVRSRLRAFPERLAACGAEAAAYGRCVQASTAPGGRLSKDFCAREFEALRSCFAAAVGQEDAGGRLLGGTLSFTPVCCHGCRALVLMAPGGTYRMPRAERKWEWRRCDIPRC

Target ORF information:

RefSeq Version	NM_001353402.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 8 (NDUFAF8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353402.1

ORF Insert Sequence:

ATGTCGGCTA ACGGAGCGGT GTGGGGCCGC GTGCGAAGCC GCCTCCGCGC CTTCCCCGAG 
CGGCTGGCCG CCTGCGGGGC CGAGGCCGCG GCGTACGGCA GGTGCGTGCA GGCCTCCACG 
GCCCCGGGCG GCCGCCTGAG TAAGGACTTC TGCGCGCGGG AGTTCGAGGC CCTGCGGAGC 
TGCTTCGCCG CTGCGGTAGG CCAAGAAGAC GCTGGAGGGA GGCTGTTAGG AGGGACTCTG 
AGCTTCACAC CTGTCTGCTG CCATGGGTGC AGAGCCCTAG TCCTGATGGC CCCTGGTGGC 
ACATATCGAA TGCCTAGGGC AGAAAGGAAG TGGGAATGGC GAAGATGTGA CATTCCTCGG 
TGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFAF8 ( NM_001086521.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001079990.1 Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 8 (NDUFAF8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu31177
Clone ID Related Accession (Same CDS sequence)	NM_001086521.1 , NM_001086521.2
Accession Version	NM_001086521.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 8 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC127837.1 and AA534550.1. On or before May 23, 2007 this sequence version replaced XM_934620.2, XM_946242.2. Transcript Variant: This variant (2) uses an alternate splice junction compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.202319.1, BC079831.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGCTA ACGGAGCGGT GTGGGGCCGC GTGCGAAGCC GCCTCCGCGC CTTCCCCGAG 
CGGCTGGCCG CCTGCGGGGC CGAGGCCGCG GCGTACGGCA GGTGCGTGCA GGCCTCCACG 
GCCCCGGGCG GCCGCCTGAG TAAGGACTTC TGCGCGCGGG AGTTCGAGGC CCTGCGGAGC 
TGCTTCGCCG CTGCGGCCAA GAAGACGCTG GAGGGAGGCT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001079990.1
CDS	54..278
Translation	MSANGAVWGRVRSRLRAFPERLAACGAEAAAYGRCVQASTAPGGRLSKDFCAREFEALRSCFAAAAKKTLEGGC

Target ORF information:

RefSeq Version	NM_001086521.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 8 (NDUFAF8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001086521.1

ORF Insert Sequence:

ATGTCGGCTA ACGGAGCGGT GTGGGGCCGC GTGCGAAGCC GCCTCCGCGC CTTCCCCGAG 
CGGCTGGCCG CCTGCGGGGC CGAGGCCGCG GCGTACGGCA GGTGCGTGCA GGCCTCCACG 
GCCCCGGGCG GCCGCCTGAG TAAGGACTTC TGCGCGCGGG AGTTCGAGGC CCTGCGGAGC 
TGCTTCGCCG CTGCGGCCAA GAAGACGCTG GAGGGAGGCT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFAF8 ( NM_001086521.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001079990.1 Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 8 (NDUFAF8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu31177
Clone ID Related Accession (Same CDS sequence)	NM_001086521.1 , NM_001086521.2
Accession Version	NM_001086521.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 8 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC127837.1, AA534550.1 and AC027601.25. On Nov 23, 2018 this sequence version replaced NM_001086521.1. Transcript Variant: This variant (2) uses an alternate splice junction compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC079831.1, SRR1163658.202319.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000431388.3/ ENSP00000400184.2 RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGGCTA ACGGAGCGGT GTGGGGCCGC GTGCGAAGCC GCCTCCGCGC CTTCCCCGAG 
CGGCTGGCCG CCTGCGGGGC CGAGGCCGCG GCGTACGGCA GGTGCGTGCA GGCCTCCACG 
GCCCCGGGCG GCCGCCTGAG TAAGGACTTC TGCGCGCGGG AGTTCGAGGC CCTGCGGAGC 
TGCTTCGCCG CTGCGGCCAA GAAGACGCTG GAGGGAGGCT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001079990.1
CDS	47..271
Translation	MSANGAVWGRVRSRLRAFPERLAACGAEAAAYGRCVQASTAPGGRLSKDFCAREFEALRSCFAAAAKKTLEGGC

Target ORF information:

RefSeq Version	NM_001086521.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 8 (NDUFAF8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001086521.2

ORF Insert Sequence:

ATGTCGGCTA ACGGAGCGGT GTGGGGCCGC GTGCGAAGCC GCCTCCGCGC CTTCCCCGAG 
CGGCTGGCCG CCTGCGGGGC CGAGGCCGCG GCGTACGGCA GGTGCGTGCA GGCCTCCACG 
GCCCCGGGCG GCCGCCTGAG TAAGGACTTC TGCGCGCGGG AGTTCGAGGC CCTGCGGAGC 
TGCTTCGCCG CTGCGGCCAA GAAGACGCTG GAGGGAGGCT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.
Molecular cell63(4)621-632(2016 Aug)
Floyd BJ,Wilkerson EM,Veling MT,Minogue CE,Xia C,Beebe ET,Wrobel RL,Cho H,Kremer LS,Alston CL,Gromek KA,Dolan BK,Ulbrich A,Stefely JA,Bohl SL,Werner KM,Jochem A,Westphall MS,Rensvold JW,Taylor RW,Prokisch H,Kim JP,Coon JJ,Pagliarini DJ

Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry.
Proceedings of the National Academy of Sciences of the United States of America104(7)2199-204(2007 Feb)
Molina H,Horn DM,Tang N,Mathivanan S,Pandey A

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NDUFAF8 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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