NSG1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NSG1
Entrez Gene ID	27065
Full Name	neuronal vesicle trafficking associated 1
Synonyms	D4S234,D4S234E,NEEP21,P21
General protein information	Preferred Names neuronal vesicle trafficking associated 1 Names neuronal vesicle trafficking-associated protein 1 neuron-specific protein family member 1 brain neuron cytoplasmic protein 1 carboxyterminally EE-tagged neuron-enriched endosomal 21 kDa protein neuron specific gene family member 1 neuron-enriched endosomal protein of 21 kD neuron-enriched endosomal protein of 21 kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4p16.3
Disorder MIM:	607645

mRNA and Protein(s)

mRNA	Protein	Name
XM_017008022.1	XP_016863511.1	neuron-specific protein family member 1 isoform X1
NM_001287763.1	NP_001274692.1	neuronal vesicle trafficking-associated protein 1 isoform a
NM_001040101.1	NP_001035190.1	neuronal vesicle trafficking-associated protein 1 isoform a
NM_001287764.1	NP_001274693.1	neuronal vesicle trafficking-associated protein 1 isoform b
NM_014392.4	NP_055207.1	neuronal vesicle trafficking-associated protein 1 isoform a

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NSG1	XP_536243.4
Mus musculus (house mouse)	Nsg1	NP_035072.2
Homo sapiens (human)	NSG1	NP_055207.1
Pan troglodytes (chimpanzee)	LOC461091	XP_001154737.2
Gallus gallus (chicken)	NSG1	NP_989896.1
	NSG1	XP_001095468.2
Rattus norvegicus (Norway rat)	Nsg1	NP_077042.2
Bos taurus (cattle)	NSG1	NP_001071425.1
Xenopus tropicalis (tropical clawed frog)	nsg1	NP_001072849.1

Gene Ontology
Bibliography

Related articles in PubMed

Complementary roles of the neuron-enriched endosomal proteins NEEP21 and calcyon in neuronal vesicle trafficking.
Muthusamy N, Chen YJ, Yin DM, Mei L, Bergson C
Journal of neurochemistry132(1)20-31(2015 Jan)

Identification of NEEP21, encoding neuron-enriched endosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53.
Ohnishi S, Futamura M, Kamino H, Nakamura Y, Kitamura N, Miyamoto Y, Miyamoto T, Shinogi D, Goda O, Arakawa H
International journal of oncology37(5)1133-41(2010 Nov)

Identification of NEEP21 as a ?-amyloid precursor protein-interacting protein in vivo that modulates amyloidogenic processing in vitro.
Norstrom EM, Zhang C, Tanzi R, Sisodia SS
The Journal of neuroscience : the official journal of the Society for Neuroscience30(46)15677-85(2010 Nov)

D4S234E, a novel p53-responsive gene, induces apoptosis in response to DNA damage.
Kudoh T, Kimura J, Lu ZG, Miki Y, Yoshida K
Experimental cell research316(17)2849-58(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This review focuses on two closely related, neuron-enriched endosomal proteins: NEEP21 and Calcyon which perform distinct roles in regulating receptor endocytosis, recycling, and degradation--{REVIEW}
Title: Complementary roles of the neuron-enriched endosomal proteins NEEP21 and calcyon in neuronal vesicle trafficking.

These results suggest that NEEP21 may play a critical role in apoptosis as a mediator of p53.
Title: Identification of NEEP21, encoding neuron-enriched endosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53.

NEEP21 profoundly affects the processing of beta-amyloid precursor proteins and Abeta peptide production.
Title: Identification of NEEP21 as a ?-amyloid precursor protein-interacting protein in vivo that modulates amyloidogenic processing in vitro.

a novel p53-responsive gene D4S234E is accumulated in the ER and induces apoptosis in response to DNA damage.
Title: D4S234E, a novel p53-responsive gene, induces apoptosis in response to DNA damage.

The following NSG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10218	NM_014392.4 Latest version!	Homo sapiens neuronal vesicle trafficking associated 1 (NSG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu10218	NM_001287763.1 Latest version!	Homo sapiens neuronal vesicle trafficking associated 1 (NSG1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu10218	NM_001040101.1 Latest version!	Homo sapiens neuronal vesicle trafficking associated 1 (NSG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu10218	XM_017008022.1 Latest version!	Homo sapiens neuron specific gene family member 1 (NSG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu12316	NM_001287764.1 Latest version!	Homo sapiens neuronal vesicle trafficking associated 1 (NSG1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSG1 ( NM_001287763.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274692.1 Homo sapiens neuronal vesicle trafficking associated 1 (NSG1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu10218
Clone ID Related Accession (Same CDS sequence)	XM_017008022.1 , NM_001287763.1 , NM_001040101.1 , NM_014392.4
Accession Version	NM_001287763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	neuronal vesicle trafficking-associated protein 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK125172.1, DC386827.1 and AC110814.5. On Dec 19, 2013 this sequence version replaced XM_005247954.1. Transcript Variant: This variant (3) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2, and 3 all encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK125172.1, SRR1803617.23932.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAGT TGGGGAACAA TTTCGCAGAG AAGGGCACCA AGCAGCCGCT GCTGGAGGAT 
GGCTTCGACA CCATTCCCCT GATGACGCCC CTCGATGTCA ATCAGCTGCA GTTCCCGCCC 
CCGGATAAGG TGGTCGTGAA AACTAAGACC GAGTATGAAC CTGACCGCAA GAAAGGGAAA 
GCACGTCCTC CCCAAATTGC TGAGTTCACC GTCAGCATCA CGGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGTTGGT CCTCTTCGCC CTGGCCTTCC TCACCTGCGT CGTCTTCCTG 
GTTGTCTACA AGGTGTACAA GTATGACCGC GCCTGCCCCG ATGGGTTCGT CCTCAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACGCGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACAGTCAT AAACCACTAC AACCTGGCCA AGCAGAGCAT CACGCGCTCC 
GTATCGCCCT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CCGAGCAGGA GACTGAAGCG 
GCTGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274692.1
CDS	470..1027
Translation	MVKLGNNFAEKGTKQPLLEDGFDTIPLMTPLDVNQLQFPPPDKVVVKTKTEYEPDRKKGKARPPQIAEFTVSITEGVTERFKVSVLVLFALAFLTCVVFLVVYKVYKYDRACPDGFVLKNTQCIPEGLESYYAEQDSSAREKFYTVINHYNLAKQSITRSVSPWMSVLSEEKLSEQETEAAEKSA

Target ORF information:

RefSeq Version	NM_001287763.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal vesicle trafficking associated 1 (NSG1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287763.1

ORF Insert Sequence:

ATGGTGAAGT TGGGGAACAA TTTCGCAGAG AAGGGCACCA AGCAGCCGCT GCTGGAGGAT 
GGCTTCGACA CCATTCCCCT GATGACGCCC CTCGATGTCA ATCAGCTGCA GTTCCCGCCC 
CCGGATAAGG TGGTCGTGAA AACTAAGACC GAGTATGAAC CTGACCGCAA GAAAGGGAAA 
GCACGTCCTC CCCAAATTGC TGAGTTCACC GTCAGCATCA CGGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGTTGGT CCTCTTCGCC CTGGCCTTCC TCACCTGCGT CGTCTTCCTG 
GTTGTCTACA AGGTGTACAA GTATGACCGC GCCTGCCCCG ATGGGTTCGT CCTCAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACGCGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACAGTCAT AAACCACTAC AACCTGGCCA AGCAGAGCAT CACGCGCTCC 
GTATCGCCCT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CCGAGCAGGA GACTGAAGCG 
GCTGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSG1 ( NM_001040101.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035190.1 Homo sapiens neuronal vesicle trafficking associated 1 (NSG1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10218
Clone ID Related Accession (Same CDS sequence)	XM_017008022.1 , NM_001287763.1 , NM_001040101.1 , NM_014392.4
Accession Version	NM_001040101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	neuronal vesicle trafficking-associated protein 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110814.5 and BC034227.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 3. Variants 1, 2, and 3 all encode the same isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1660807.41758.1, SRR1803614.108440.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAGT TGGGGAACAA TTTCGCAGAG AAGGGCACCA AGCAGCCGCT GCTGGAGGAT 
GGCTTCGACA CCATTCCCCT GATGACGCCC CTCGATGTCA ATCAGCTGCA GTTCCCGCCC 
CCGGATAAGG TGGTCGTGAA AACTAAGACC GAGTATGAAC CTGACCGCAA GAAAGGGAAA 
GCACGTCCTC CCCAAATTGC TGAGTTCACC GTCAGCATCA CGGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGTTGGT CCTCTTCGCC CTGGCCTTCC TCACCTGCGT CGTCTTCCTG 
GTTGTCTACA AGGTGTACAA GTATGACCGC GCCTGCCCCG ATGGGTTCGT CCTCAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACGCGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACAGTCAT AAACCACTAC AACCTGGCCA AGCAGAGCAT CACGCGCTCC 
GTATCGCCCT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CCGAGCAGGA GACTGAAGCG 
GCTGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035190.1
CDS	415..972
Translation	MVKLGNNFAEKGTKQPLLEDGFDTIPLMTPLDVNQLQFPPPDKVVVKTKTEYEPDRKKGKARPPQIAEFTVSITEGVTERFKVSVLVLFALAFLTCVVFLVVYKVYKYDRACPDGFVLKNTQCIPEGLESYYAEQDSSAREKFYTVINHYNLAKQSITRSVSPWMSVLSEEKLSEQETEAAEKSA

Target ORF information:

RefSeq Version	NM_001040101.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal vesicle trafficking associated 1 (NSG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040101.1

ORF Insert Sequence:

ATGGTGAAGT TGGGGAACAA TTTCGCAGAG AAGGGCACCA AGCAGCCGCT GCTGGAGGAT 
GGCTTCGACA CCATTCCCCT GATGACGCCC CTCGATGTCA ATCAGCTGCA GTTCCCGCCC 
CCGGATAAGG TGGTCGTGAA AACTAAGACC GAGTATGAAC CTGACCGCAA GAAAGGGAAA 
GCACGTCCTC CCCAAATTGC TGAGTTCACC GTCAGCATCA CGGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGTTGGT CCTCTTCGCC CTGGCCTTCC TCACCTGCGT CGTCTTCCTG 
GTTGTCTACA AGGTGTACAA GTATGACCGC GCCTGCCCCG ATGGGTTCGT CCTCAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACGCGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACAGTCAT AAACCACTAC AACCTGGCCA AGCAGAGCAT CACGCGCTCC 
GTATCGCCCT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CCGAGCAGGA GACTGAAGCG 
GCTGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSG1 ( NM_014392.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055207.1 Homo sapiens neuronal vesicle trafficking associated 1 (NSG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10218
Clone ID Related Accession (Same CDS sequence)	XM_017008022.1 , NM_001287763.1 , NM_001040101.1 , NM_014392.4
Accession Version	NM_014392.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	neuronal vesicle trafficking-associated protein 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY141242.1, DA773692.1, AK125172.1 and AC110814.5. On Dec 19, 2013 this sequence version replaced NM_014392.3. Transcript Variant: This variant (1) differs in the 5' UTR compared to variant (3). Variants 1, 2, and 3 all encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.206931.1, SRR1803612.22644.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAGT TGGGGAACAA TTTCGCAGAG AAGGGCACCA AGCAGCCGCT GCTGGAGGAT 
GGCTTCGACA CCATTCCCCT GATGACGCCC CTCGATGTCA ATCAGCTGCA GTTCCCGCCC 
CCGGATAAGG TGGTCGTGAA AACTAAGACC GAGTATGAAC CTGACCGCAA GAAAGGGAAA 
GCACGTCCTC CCCAAATTGC TGAGTTCACC GTCAGCATCA CGGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGTTGGT CCTCTTCGCC CTGGCCTTCC TCACCTGCGT CGTCTTCCTG 
GTTGTCTACA AGGTGTACAA GTATGACCGC GCCTGCCCCG ATGGGTTCGT CCTCAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACGCGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACAGTCAT AAACCACTAC AACCTGGCCA AGCAGAGCAT CACGCGCTCC 
GTATCGCCCT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CCGAGCAGGA GACTGAAGCG 
GCTGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055207.1
CDS	238..795
Translation	MVKLGNNFAEKGTKQPLLEDGFDTIPLMTPLDVNQLQFPPPDKVVVKTKTEYEPDRKKGKARPPQIAEFTVSITEGVTERFKVSVLVLFALAFLTCVVFLVVYKVYKYDRACPDGFVLKNTQCIPEGLESYYAEQDSSAREKFYTVINHYNLAKQSITRSVSPWMSVLSEEKLSEQETEAAEKSA

Target ORF information:

RefSeq Version	NM_014392.4
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal vesicle trafficking associated 1 (NSG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014392.4

ORF Insert Sequence:

ATGGTGAAGT TGGGGAACAA TTTCGCAGAG AAGGGCACCA AGCAGCCGCT GCTGGAGGAT 
GGCTTCGACA CCATTCCCCT GATGACGCCC CTCGATGTCA ATCAGCTGCA GTTCCCGCCC 
CCGGATAAGG TGGTCGTGAA AACTAAGACC GAGTATGAAC CTGACCGCAA GAAAGGGAAA 
GCACGTCCTC CCCAAATTGC TGAGTTCACC GTCAGCATCA CGGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGTTGGT CCTCTTCGCC CTGGCCTTCC TCACCTGCGT CGTCTTCCTG 
GTTGTCTACA AGGTGTACAA GTATGACCGC GCCTGCCCCG ATGGGTTCGT CCTCAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACGCGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACAGTCAT AAACCACTAC AACCTGGCCA AGCAGAGCAT CACGCGCTCC 
GTATCGCCCT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CCGAGCAGGA GACTGAAGCG 
GCTGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSG1 ( XM_017008022.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863511.1 Homo sapiens neuron specific gene family member 1 (NSG1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu10218
Clone ID Related Accession (Same CDS sequence)	XM_017008022.1 , NM_001287763.1 , NM_001040101.1 , NM_014392.4
Accession Version	XM_017008022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neuron-specific protein family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGT TGGGGAACAA TTTCGCAGAG AAGGGCACCA AGCAGCCGCT GCTGGAGGAT 
GGCTTCGACA CCATTCCCCT GATGACGCCC CTCGATGTCA ATCAGCTGCA GTTCCCGCCC 
CCGGATAAGG TGGTCGTGAA AACTAAGACC GAGTATGAAC CTGACCGCAA GAAAGGGAAA 
GCACGTCCTC CCCAAATTGC TGAGTTCACC GTCAGCATCA CGGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGTTGGT CCTCTTCGCC CTGGCCTTCC TCACCTGCGT CGTCTTCCTG 
GTTGTCTACA AGGTGTACAA GTATGACCGC GCCTGCCCCG ATGGGTTCGT CCTCAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACGCGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACAGTCAT AAACCACTAC AACCTGGCCA AGCAGAGCAT CACGCGCTCC 
GTATCGCCCT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CCGAGCAGGA GACTGAAGCG 
GCTGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863511.1
CDS	1655..2212
Translation	MVKLGNNFAEKGTKQPLLEDGFDTIPLMTPLDVNQLQFPPPDKVVVKTKTEYEPDRKKGKARPPQIAEFTVSITEGVTERFKVSVLVLFALAFLTCVVFLVVYKVYKYDRACPDGFVLKNTQCIPEGLESYYAEQDSSAREKFYTVINHYNLAKQSITRSVSPWMSVLSEEKLSEQETEAAEKSA

Target ORF information:

RefSeq Version	XM_017008022.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neuron specific gene family member 1 (NSG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008022.1

ORF Insert Sequence:

ATGGTGAAGT TGGGGAACAA TTTCGCAGAG AAGGGCACCA AGCAGCCGCT GCTGGAGGAT 
GGCTTCGACA CCATTCCCCT GATGACGCCC CTCGATGTCA ATCAGCTGCA GTTCCCGCCC 
CCGGATAAGG TGGTCGTGAA AACTAAGACC GAGTATGAAC CTGACCGCAA GAAAGGGAAA 
GCACGTCCTC CCCAAATTGC TGAGTTCACC GTCAGCATCA CGGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGTTGGT CCTCTTCGCC CTGGCCTTCC TCACCTGCGT CGTCTTCCTG 
GTTGTCTACA AGGTGTACAA GTATGACCGC GCCTGCCCCG ATGGGTTCGT CCTCAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACGCGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACAGTCAT AAACCACTAC AACCTGGCCA AGCAGAGCAT CACGCGCTCC 
GTATCGCCCT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CCGAGCAGGA GACTGAAGCG 
GCTGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSG1 ( NM_001287764.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274693.1 Homo sapiens neuronal vesicle trafficking associated 1 (NSG1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu12316
Clone ID Related Accession (Same CDS sequence)	NM_001287764.1
Accession Version	NM_001287764.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	neuronal vesicle trafficking-associated protein 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK125172.1, DC386827.1, DA746130.1 and AC110814.5. Transcript Variant: This variant (4) lacks an alternate exon in the 5' UTR and uses an alternate splice junction at the 5' end of an exon compared to variant 3. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: DA488556.1, DA746130.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGCCCC TCGATGTCAA TCAGCTGCAG TTCCCGCCCC CGGATAAGGT GGTCGTGAAA 
ACTAAGACCG AGTATGAACC TGACCGCAAG AAAGGGAAAG CACGTCCTCC CCAAATTGCT 
GAGTTCACCG TCAGCATCAC GGAGGGTGTC ACCGAGAGGT TTAAGGTCTC CGTGTTGGTC 
CTCTTCGCCC TGGCCTTCCT CACCTGCGTC GTCTTCCTGG TTGTCTACAA GGTGTACAAG 
TATGACCGCG CCTGCCCCGA TGGGTTCGTC CTCAAGAACA CCCAGTGCAT CCCAGAAGGC 
TTGGAGAGCT ACTACGCGGA GCAAGACTCC AGTGCCCGGG AGAAATTTTA CACAGTCATA 
AACCACTACA ACCTGGCCAA GCAGAGCATC ACGCGCTCCG TATCGCCCTG GATGTCAGTT 
CTGTCAGAAG AGAAGCTGTC CGAGCAGGAG ACTGAAGCGG CTGAGAAGTC AGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274693.1
CDS	442..918
Translation	MTPLDVNQLQFPPPDKVVVKTKTEYEPDRKKGKARPPQIAEFTVSITEGVTERFKVSVLVLFALAFLTCVVFLVVYKVYKYDRACPDGFVLKNTQCIPEGLESYYAEQDSSAREKFYTVINHYNLAKQSITRSVSPWMSVLSEEKLSEQETEAAEKSA

Target ORF information:

RefSeq Version	NM_001287764.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal vesicle trafficking associated 1 (NSG1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287764.1

ORF Insert Sequence:

ATGACGCCCC TCGATGTCAA TCAGCTGCAG TTCCCGCCCC CGGATAAGGT GGTCGTGAAA 
ACTAAGACCG AGTATGAACC TGACCGCAAG AAAGGGAAAG CACGTCCTCC CCAAATTGCT 
GAGTTCACCG TCAGCATCAC GGAGGGTGTC ACCGAGAGGT TTAAGGTCTC CGTGTTGGTC 
CTCTTCGCCC TGGCCTTCCT CACCTGCGTC GTCTTCCTGG TTGTCTACAA GGTGTACAAG 
TATGACCGCG CCTGCCCCGA TGGGTTCGTC CTCAAGAACA CCCAGTGCAT CCCAGAAGGC 
TTGGAGAGCT ACTACGCGGA GCAAGACTCC AGTGCCCGGG AGAAATTTTA CACAGTCATA 
AACCACTACA ACCTGGCCAA GCAGAGCATC ACGCGCTCCG TATCGCCCTG GATGTCAGTT 
CTGTCAGAAG AGAAGCTGTC CGAGCAGGAG ACTGAAGCGG CTGAGAAGTC AGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Complementary roles of the neuron-enriched endosomal proteins NEEP21 and calcyon in neuronal vesicle trafficking.
Journal of neurochemistry132(1)20-31(2015 Jan)
Muthusamy N,Chen YJ,Yin DM,Mei L,Bergson C

Identification of NEEP21, encoding neuron-enriched endosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53.
International journal of oncology37(5)1133-41(2010 Nov)
Ohnishi S,Futamura M,Kamino H,Nakamura Y,Kitamura N,Miyamoto Y,Miyamoto T,Shinogi D,Goda O,Arakawa H

Identification of NEEP21 as a ?-amyloid precursor protein-interacting protein in vivo that modulates amyloidogenic processing in vitro.
The Journal of neuroscience : the official journal of the Society for Neuroscience30(46)15677-85(2010 Nov)
Norstrom EM,Zhang C,Tanzi R,Sisodia SS

D4S234E, a novel p53-responsive gene, induces apoptosis in response to DNA damage.
Experimental cell research316(17)2849-58(2010 Oct)
Kudoh T,Kimura J,Lu ZG,Miki Y,Yoshida K

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NSG1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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