GCSH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCSH
Entrez Gene ID	2653
Full Name	glycine cleavage system protein H
Synonyms	GCE,NKH
General protein information	Preferred Names glycine cleavage system protein H Names glycine cleavage system H protein, mitochondrial glycine cleavage system protein H (aminomethyl carrier) lipoic acid-containing protein mitochondrial glycine cleavage system H-protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q23.2
Summary	Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH). Two transcript variants, one protein-coding and the other probably not protein-coding,have been found for this gene. Also, several transcribed and non-transcribed pseudogenes of this gene exist throughout the genome.[provided by RefSeq, Jan 2010].
Disorder MIM:	238330
Disorder Html:	Glycine encephalopathy, 605899 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004483.4	NP_004474.2	glycine cleavage system H protein, mitochondrial precursor
NM_004483.5	NP_004474.2	glycine cleavage system H protein, mitochondrial precursor
XM_017023137.1	XP_016878626.1	glycine cleavage system H protein, mitochondrial isoform X2
XM_017023136.2	XP_016878625.1	glycine cleavage system H protein, mitochondrial isoform X1
XM_017023136.1	XP_016878625.1	glycine cleavage system H protein, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	GCSH	NP_004474.2
Pan troglodytes (chimpanzee)	GCSH	XP_001160723.1
Danio rerio (zebrafish)	gcshb	NP_001002579.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006548	XP_316586.4
Magnaporthe oryzae (rice blast fungus)	MGG_04861	XP_003712341.1
Arabidopsis thaliana (thale cress)	AT2G35120	NP_181057.1
Canis lupus familiaris (dog)	GCSH	XP_536768.2
Bos taurus (cattle)	LOC101905887	XP_005207742.1
Rattus norvegicus (Norway rat)	Gcsh	NP_598282.2
Xenopus tropicalis (tropical clawed frog)	gcsh	NP_001011024.1
Bos taurus (cattle)	GCSH	NP_777269.1
Neurospora crassa	NCU08877	XP_960670.1
Oryza sativa (rice)	Os06g0667600	NP_001058315.1
Mus musculus (house mouse)	Gcsh	NP_080848.1
Danio rerio (zebrafish)	gcsha	NP_001013475.1
Caenorhabditis elegans (roundworm)	gcsh-1	NP_510414.1
Saccharomyces cerevisiae (baker's yeast)	GCV3	NP_009355.3
Schizosaccharomyces pombe (fission yeast)	gcv3	NP_596169.1
Gallus gallus (chicken)	GCSH	NP_001004372.1
Drosophila melanogaster (fruit fly)	ppl	NP_524197.1
Caenorhabditis elegans (roundworm)	F52A8.5	NP_492075.1
Kluyveromyces lactis	KLLA0A03597g	XP_451158.1
Eremothecium gossypii	AGOS_ADR396W	NP_984491.2
Oryza sativa (rice)	Os02g0170100	NP_001046026.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutation analysis of glycine decarboxylase, aminomethyltransferase and glycine cleavage system protein-H genes in 13 unrelated families with glycine encephalopathy.
Azize NA, Ngah WZ, Othman Z, Md Desa N, Chin CB, Md Yunus Z, Mohan A, Hean TS, Syed Zakaria SZ, Lock-Hock N
Journal of human genetics59(11)593-7(2014 Nov)

Glycine cleavage enzyme complex: molecular cloning and expression of the H-protein cDNA from cultured human skin fibroblasts.
Zay A, Choy FY, Patrick C, Sinclair G
Biochemistry and cell biology = Biochimie et biologie cellulaire89(3)299-307(2011 Jun)

Fine mapping and association studies of a high-density lipoprotein cholesterol linkage region on chromosome 16 in French-Canadian subjects.
Dastani Z, Pajukanta P, Marcil M, Rudzicz N, Ruel I, Bailey SD, Lee JC, Lemire M, Faith J, Platko J, Rioux J, Hudson TJ, Gaudet D, Engert JC, Genest J
European journal of human genetics : EJHG18(3)342-7(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate no mutation was found in glycine cleavage system protein-H (GCSH) and suggest that mutations in both glycine decarboxylase (GLDC) and aminomethyltransferase (AMT) are the main cause of glycine encephalopathy in Malaysian population.
Title: Mutation analysis of glycine decarboxylase, aminomethyltransferase and glycine cleavage system protein-H genes in 13 unrelated families with glycine encephalopathy.

There is no detectable glycine cleavage enzyme activity in human skin fibroblasts.
Title: Glycine cleavage enzyme complex: molecular cloning and expression of the H-protein cDNA from cultured human skin fibroblasts.

Observational study of gene-disease association. (HuGE Navigator)
Title: Fine mapping and association studies of a high-density lipoprotein cholesterol linkage region on chromosome 16 in French-Canadian subjects.

Genetic analysis showed a non-previously described mutation affecting a consensus splice site (IVS2-1G > C 3) in the AMT gene encoding the T protein of the glycine cleavage system.
Title: Atypical glycine encephalopathy in an extremely low birth weight infant: description of a new mutation and clinical and electroencephalographic analysis.

Observational study of genotype prevalence. (HuGE Navigator)
Title: Comprehensive mutation analysis of GLDC, AMT, and GCSH in nonketotic hyperglycinemia.

The following GCSH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCSH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21473	NM_004483.5 Latest version!	Homo sapiens glycine cleavage system protein H (GCSH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu85968	XM_017023137.1 Latest version!	Homo sapiens glycine cleavage system protein H (GCSH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu85967	XM_017023136.2 Latest version!	Homo sapiens glycine cleavage system protein H (GCSH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
View Old Accession Versions >>
OHu21473	NM_004483.4	Homo sapiens glycine cleavage system protein H (GCSH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu85967	XM_017023136.1	Homo sapiens glycine cleavage system protein H (GCSH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCSH ( NM_004483.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004474.2 Homo sapiens glycine cleavage system protein H (GCSH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21473
Clone ID Related Accession (Same CDS sequence)	NM_004483.4 , NM_004483.5
Accession Version	NM_004483.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	glycine cleavage system H protein, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN283809.1, AV696698.1, BF798008.1 and AC092718.3. This sequence is a reference standard in the RefSeqGene project. On Jan 16, 2010 this sequence version replaced NM_004483.3. Transcript Variant: This variant (1) represents the longer transcript and is the protein-coding variant. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.414711.1, SRR1660809.336720.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGCTGC GAGTGGTGCG GAGCGTGCGG GCCCTGCTCT GCACCCTGCG CGCGGTCCCG 
TCACCCGCCG CGCCCTGCCC GCCGAGGCCC TGGCAGCTGG GGGTGGGCGC CGTCCGTACG 
CTGCGCACTG GACCCGCTCT GCTCTCGGTG CGTAAATTCA CAGAGAAACA CGAATGGGTA 
ACAACAGAAA ATGGCATTGG AACAGTGGGA ATCAGCAATT TTGCACAGGA AGCGTTGGGA 
GATGTTGTTT ATTGTAGTCT CCCTGAAGTT GGGACAAAAT TGAACAAACA AGATGAGTTT 
GGTGCTTTGG AAAGTGTGAA AGCTGCTAGT GAACTCTATT CTCCTTTATC AGGAGAAGTA 
ACTGAAATTA ATGAAGCTCT TGCAGAAAAT CCAGGACTTG TAAACAAATC TTGTTATGAA 
GATGGTTGGC TGATCAAGAT GACACTGAGT AACCCTTCAG AACTAGATGA ACTTATGAGT 
GAAGAAGCAT ATGAGAAATA CATAAAATCT ATTGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004474.2
CDS	98..619
Translation	MALRVVRSVRALLCTLRAVPSPAAPCPPRPWQLGVGAVRTLRTGPALLSVRKFTEKHEWVTTENGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAENPGLVNKSCYEDGWLIKMTLSNPSELDELMSEEAYEKYIKSIEE

Target ORF information:

RefSeq Version	NM_004483.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glycine cleavage system protein H (GCSH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004483.4

ORF Insert Sequence:

ATGGCGCTGC GAGTGGTGCG GAGCGTGCGG GCCCTGCTCT GCACCCTGCG CGCGGTCCCG 
TCACCCGCCG CGCCCTGCCC GCCGAGGCCC TGGCAGCTGG GGGTGGGCGC CGTCCGTACG 
CTGCGCACTG GACCCGCTCT GCTCTCGGTG CGTAAATTCA CAGAGAAACA CGAATGGGTA 
ACAACAGAAA ATGGCATTGG AACAGTGGGA ATCAGCAATT TTGCACAGGA AGCGTTGGGA 
GATGTTGTTT ATTGTAGTCT CCCTGAAGTT GGGACAAAAT TGAACAAACA AGATGAGTTT 
GGTGCTTTGG AAAGTGTGAA AGCTGCTAGT GAACTCTATT CTCCTTTATC AGGAGAAGTA 
ACTGAAATTA ATGAAGCTCT TGCAGAAAAT CCAGGACTTG TAAACAAATC TTGTTATGAA 
GATGGTTGGC TGATCAAGAT GACACTGAGT AACCCTTCAG AACTAGATGA ACTTATGAGT 
GAAGAAGCAT ATGAGAAATA CATAAAATCT ATTGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCSH ( NM_004483.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004474.2 Homo sapiens glycine cleavage system protein H (GCSH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21473
Clone ID Related Accession (Same CDS sequence)	NM_004483.4 , NM_004483.5
Accession Version	NM_004483.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	glycine cleavage system H protein, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092718.3, CN283809.1, AV696698.1 and BF798008.1. On Aug 31, 2019 this sequence version replaced NM_004483.4. Transcript Variant: This variant (1) represents the longer transcript and is the protein-coding variant. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009065.1, M69175.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGCTGC GAGTGGTGCG GAGCGTGCGG GCCCTGCTCT GCACCCTGCG CGCGGTCCCG 
TCACCCGCCG CGCCCTGCCC GCCGAGGCCC TGGCAGCTGG GGGTGGGCGC CGTCCGTACG 
CTGCGCACTG GACCCGCTCT GCTCTCGGTG CGTAAATTCA CAGAGAAACA CGAATGGGTA 
ACAACAGAAA ATGGCATTGG AACAGTGGGA ATCAGCAATT TTGCACAGGA AGCGTTGGGA 
GATGTTGTTT ATTGTAGTCT CCCTGAAGTT GGGACAAAAT TGAACAAACA AGATGAGTTT 
GGTGCTTTGG AAAGTGTGAA AGCTGCTAGT GAACTCTATT CTCCTTTATC AGGAGAAGTA 
ACTGAAATTA ATGAAGCTCT TGCAGAAAAT CCAGGACTTG TAAACAAATC TTGTTATGAA 
GATGGTTGGC TGATCAAGAT GACACTGAGT AACCCTTCAG AACTAGATGA ACTTATGAGT 
GAAGAAGCAT ATGAGAAATA CATAAAATCT ATTGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004474.2
CDS	118..639
Translation	MALRVVRSVRALLCTLRAVPSPAAPCPPRPWQLGVGAVRTLRTGPALLSVRKFTEKHEWVTTENGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAENPGLVNKSCYEDGWLIKMTLSNPSELDELMSEEAYEKYIKSIEE

Target ORF information:

RefSeq Version	NM_004483.5
Organism	Homo sapiens(human)
Definition	Homo sapiens glycine cleavage system protein H (GCSH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004483.5

ORF Insert Sequence:

ATGGCGCTGC GAGTGGTGCG GAGCGTGCGG GCCCTGCTCT GCACCCTGCG CGCGGTCCCG 
TCACCCGCCG CGCCCTGCCC GCCGAGGCCC TGGCAGCTGG GGGTGGGCGC CGTCCGTACG 
CTGCGCACTG GACCCGCTCT GCTCTCGGTG CGTAAATTCA CAGAGAAACA CGAATGGGTA 
ACAACAGAAA ATGGCATTGG AACAGTGGGA ATCAGCAATT TTGCACAGGA AGCGTTGGGA 
GATGTTGTTT ATTGTAGTCT CCCTGAAGTT GGGACAAAAT TGAACAAACA AGATGAGTTT 
GGTGCTTTGG AAAGTGTGAA AGCTGCTAGT GAACTCTATT CTCCTTTATC AGGAGAAGTA 
ACTGAAATTA ATGAAGCTCT TGCAGAAAAT CCAGGACTTG TAAACAAATC TTGTTATGAA 
GATGGTTGGC TGATCAAGAT GACACTGAGT AACCCTTCAG AACTAGATGA ACTTATGAGT 
GAAGAAGCAT ATGAGAAATA CATAAAATCT ATTGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCSH ( XM_017023137.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878626.1 Homo sapiens glycine cleavage system protein H (GCSH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu85968
Clone ID Related Accession (Same CDS sequence)	XM_017023137.1
Accession Version	XM_017023137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	glycine cleavage system H protein, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCATC TTTATGCTGC CTCCCTAAAC CTCATCACCC CAAATTTGTC TTCAGAGGCA 
CAGATTTTTT TTTCTGCAAG ACAGACCAGT AATATGCGTA AATTCACAGA GAAACACGAA 
TGGGTAACAA CAGAAAATGG CATTGGAACA GTGGGAATCA GCAATTTTGC ACAGGAAGCG 
TTGGGAGATG TTGTTTATTG TAGTCTCCCT GAAGTTGGGA CAAAATTGAA CAAACAAGAT 
GAGTTTGGTG CTTTGGAAAG TGTGAAAGCT GCTAGTGAAC TCTATTCTCC TTTATCAGGA 
GAAGTAACTG AAATTAATGA AGCTCTTGCA GAAAATCCAG GACTTGTAAA CAAATCTTGT 
TATGAAGATG GTTGTCTTTT GGTTGCAGGT TGGCTGATCA AGATGACACT GAGTAACCCT 
TCAGAACTAG ATGAACTTAT GAGTGAAGAA GCATATGAGA AATACATAAA ATCTATTGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878626.1
CDS	94..579
Translation	MSHLYAASLNLITPNLSSEAQIFFSARQTSNMRKFTEKHEWVTTENGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAENPGLVNKSCYEDGCLLVAGWLIKMTLSNPSELDELMSEEAYEKYIKSIEE

Target ORF information:

RefSeq Version	XM_017023137.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glycine cleavage system protein H (GCSH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023137.1

ORF Insert Sequence:

ATGAGCCATC TTTATGCTGC CTCCCTAAAC CTCATCACCC CAAATTTGTC TTCAGAGGCA 
CAGATTTTTT TTTCTGCAAG ACAGACCAGT AATATGCGTA AATTCACAGA GAAACACGAA 
TGGGTAACAA CAGAAAATGG CATTGGAACA GTGGGAATCA GCAATTTTGC ACAGGAAGCG 
TTGGGAGATG TTGTTTATTG TAGTCTCCCT GAAGTTGGGA CAAAATTGAA CAAACAAGAT 
GAGTTTGGTG CTTTGGAAAG TGTGAAAGCT GCTAGTGAAC TCTATTCTCC TTTATCAGGA 
GAAGTAACTG AAATTAATGA AGCTCTTGCA GAAAATCCAG GACTTGTAAA CAAATCTTGT 
TATGAAGATG GTTGTCTTTT GGTTGCAGGT TGGCTGATCA AGATGACACT GAGTAACCCT 
TCAGAACTAG ATGAACTTAT GAGTGAAGAA GCATATGAGA AATACATAAA ATCTATTGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCSH ( XM_017023136.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878625.1 Homo sapiens glycine cleavage system protein H (GCSH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu85967
Clone ID Related Accession (Same CDS sequence)	XM_017023136.2 , XM_017023136.1
Accession Version	XM_017023136.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	glycine cleavage system H protein, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017023136.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGC GAGTGGTGCG GAGCGTGCGG GCCCTGCTCT GCACCCTGCG CGCGGTCCCG 
TCACCCGCCG CGCCCTGCCC GCCGAGGCCC TGGCAGCTGG GGGTGGGCGC CGTCCGTACG 
CTGCGCACTG GACCCGCTCT GCTCTCGGTG CGTAAATTCA CAGAGAAACA CGAATGGGTA 
ACAACAGAAA ATGGCATTGG AACAGTGGGA ATCAGCAATT TTGCACAGGA AGCGTTGGGA 
GATGTTGTTT ATTGTAGTCT CCCTGAAGTT GGGACAAAAT TGAACAAACA AGATGAGTTT 
GGTGCTTTGG AAAGTGTGAA AGCTGCTAGT GAACTCTATT CTCCTTTATC AGGAGAAGTA 
ACTGAAATTA ATGAAGCTCT TGCAGAAAAT CCAGGACTTG TAAACAAATC TTGTTATGAA 
GATGGTTGTC TTTTGGTTGC AGGTTGGCTG ATCAAGATGA CACTGAGTAA CCCTTCAGAA 
CTAGATGAAC TTATGAGTGA AGAAGCATAT GAGAAATACA TAAAATCTAT TGAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878625.1
CDS	22..561
Translation	MALRVVRSVRALLCTLRAVPSPAAPCPPRPWQLGVGAVRTLRTGPALLSVRKFTEKHEWVTTENGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAENPGLVNKSCYEDGCLLVAGWLIKMTLSNPSELDELMSEEAYEKYIKSIEE

Target ORF information:

RefSeq Version	XM_017023136.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glycine cleavage system protein H (GCSH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023136.2

ORF Insert Sequence:

ATGGCGCTGC GAGTGGTGCG GAGCGTGCGG GCCCTGCTCT GCACCCTGCG CGCGGTCCCG 
TCACCCGCCG CGCCCTGCCC GCCGAGGCCC TGGCAGCTGG GGGTGGGCGC CGTCCGTACG 
CTGCGCACTG GACCCGCTCT GCTCTCGGTG CGTAAATTCA CAGAGAAACA CGAATGGGTA 
ACAACAGAAA ATGGCATTGG AACAGTGGGA ATCAGCAATT TTGCACAGGA AGCGTTGGGA 
GATGTTGTTT ATTGTAGTCT CCCTGAAGTT GGGACAAAAT TGAACAAACA AGATGAGTTT 
GGTGCTTTGG AAAGTGTGAA AGCTGCTAGT GAACTCTATT CTCCTTTATC AGGAGAAGTA 
ACTGAAATTA ATGAAGCTCT TGCAGAAAAT CCAGGACTTG TAAACAAATC TTGTTATGAA 
GATGGTTGTC TTTTGGTTGC AGGTTGGCTG ATCAAGATGA CACTGAGTAA CCCTTCAGAA 
CTAGATGAAC TTATGAGTGA AGAAGCATAT GAGAAATACA TAAAATCTAT TGAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCSH ( XM_017023136.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878625.1 Homo sapiens glycine cleavage system protein H (GCSH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu85967
Clone ID Related Accession (Same CDS sequence)	XM_017023136.2 , XM_017023136.1
Accession Version	XM_017023136.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	glycine cleavage system H protein, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGC GAGTGGTGCG GAGCGTGCGG GCCCTGCTCT GCACCCTGCG CGCGGTCCCG 
TCACCCGCCG CGCCCTGCCC GCCGAGGCCC TGGCAGCTGG GGGTGGGCGC CGTCCGTACG 
CTGCGCACTG GACCCGCTCT GCTCTCGGTG CGTAAATTCA CAGAGAAACA CGAATGGGTA 
ACAACAGAAA ATGGCATTGG AACAGTGGGA ATCAGCAATT TTGCACAGGA AGCGTTGGGA 
GATGTTGTTT ATTGTAGTCT CCCTGAAGTT GGGACAAAAT TGAACAAACA AGATGAGTTT 
GGTGCTTTGG AAAGTGTGAA AGCTGCTAGT GAACTCTATT CTCCTTTATC AGGAGAAGTA 
ACTGAAATTA ATGAAGCTCT TGCAGAAAAT CCAGGACTTG TAAACAAATC TTGTTATGAA 
GATGGTTGTC TTTTGGTTGC AGGTTGGCTG ATCAAGATGA CACTGAGTAA CCCTTCAGAA 
CTAGATGAAC TTATGAGTGA AGAAGCATAT GAGAAATACA TAAAATCTAT TGAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878625.1
CDS	97..636
Translation	MALRVVRSVRALLCTLRAVPSPAAPCPPRPWQLGVGAVRTLRTGPALLSVRKFTEKHEWVTTENGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAENPGLVNKSCYEDGCLLVAGWLIKMTLSNPSELDELMSEEAYEKYIKSIEE

Target ORF information:

RefSeq Version	XM_017023136.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glycine cleavage system protein H (GCSH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023136.1

ORF Insert Sequence:

ATGGCGCTGC GAGTGGTGCG GAGCGTGCGG GCCCTGCTCT GCACCCTGCG CGCGGTCCCG 
TCACCCGCCG CGCCCTGCCC GCCGAGGCCC TGGCAGCTGG GGGTGGGCGC CGTCCGTACG 
CTGCGCACTG GACCCGCTCT GCTCTCGGTG CGTAAATTCA CAGAGAAACA CGAATGGGTA 
ACAACAGAAA ATGGCATTGG AACAGTGGGA ATCAGCAATT TTGCACAGGA AGCGTTGGGA 
GATGTTGTTT ATTGTAGTCT CCCTGAAGTT GGGACAAAAT TGAACAAACA AGATGAGTTT 
GGTGCTTTGG AAAGTGTGAA AGCTGCTAGT GAACTCTATT CTCCTTTATC AGGAGAAGTA 
ACTGAAATTA ATGAAGCTCT TGCAGAAAAT CCAGGACTTG TAAACAAATC TTGTTATGAA 
GATGGTTGTC TTTTGGTTGC AGGTTGGCTG ATCAAGATGA CACTGAGTAA CCCTTCAGAA 
CTAGATGAAC TTATGAGTGA AGAAGCATAT GAGAAATACA TAAAATCTAT TGAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutation analysis of glycine decarboxylase, aminomethyltransferase and glycine cleavage system protein-H genes in 13 unrelated families with glycine encephalopathy.
Journal of human genetics59(11)593-7(2014 Nov)
Azize NA,Ngah WZ,Othman Z,Md Desa N,Chin CB,Md Yunus Z,Mohan A,Hean TS,Syed Zakaria SZ,Lock-Hock N

Glycine cleavage enzyme complex: molecular cloning and expression of the H-protein cDNA from cultured human skin fibroblasts.
Biochemistry and cell biology = Biochimie et biologie cellulaire89(3)299-307(2011 Jun)
Zay A,Choy FY,Patrick C,Sinclair G

Fine mapping and association studies of a high-density lipoprotein cholesterol linkage region on chromosome 16 in French-Canadian subjects.
European journal of human genetics : EJHG18(3)342-7(2010 Mar)
Dastani Z,Pajukanta P,Marcil M,Rudzicz N,Ruel I,Bailey SD,Lee JC,Lemire M,Faith J,Platko J,Rioux J,Hudson TJ,Gaudet D,Engert JC,Genest J

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GCSH cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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