GCG cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCG
Entrez Gene ID	2641
Full Name	glucagon
Synonyms	GLP-1,GLP1,GLP2,GRPP
General protein information	Preferred Names glucagon Names glucagon glicentin-related polypeptide glucagon-like peptide 1 glucagon-like peptide 2 preproglucagon
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q24.2
Summary	The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. [provided by RefSeq, Jul 2008].
Disorder MIM:	138030
Disorder Html:	[?Hyperproglucagonemia] (1)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002054.4	NP_002045.1	glucagon preproprotein
NM_002054.5	NP_002045.1	glucagon preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	GCG	NP_002045.1
Danio rerio (zebrafish)	gcgb	NP_001229699.1

Gene Ontology
Bibliography

Related articles in PubMed

GLP-1 response to sequential mixed meals: influence of insulin resistance.
Rebelos E, Astiarraga B, Bizzotto R, Mari A, Manca ML, Gonzalez A, Mendez A, Martinez CA, Hurwitz BE, Ferrannini E
Clinical science (London, England : 1979)131(24)2901-2910(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Insulin resistance in non-diabetic individuals is associated with raised fasting GLP-1 levels but reduced GLP-1 responses to meal stimulation.
Title: GLP-1 response to sequential mixed meals: influence of insulin resistance.

Data suggest early peaks in glucagon-like peptide-1 and glucagon secretion/blood level together trigger exaggerated insulinotropic response (high insulin secretion/level) to eating and consequent hypoglycaemia in patients with postprandial hypoglycaemia as a postoperative complication following Roux-en-Y gastric bypass for obesity complicated by type 2 diabetes; this retrospective cohort study was conducted in London.
Title: Roles of increased glycaemic variability, GLP-1 and glucagon in hypoglycaemia after Roux-en-Y gastric bypass.

Studies indicate that nutrient-induced glucagonlike peptide-1 (GLP-1) response was one of the best predictors of type 2 diabetes mellitus (T2DM) remission after Roux-en-Y-gastric-bypass (RYGB).
Title: The Role of GLP-1 in the Metabolic Success of Bariatric Surgery.

Age-dependent human beta cell proliferation induced by glucagon-like peptide 1 and calcineurin signaling
Title: Age-dependent human ? cell proliferation induced by glucagon-like peptide 1 and calcineurin signaling.

A common variant, i.e., single nucleotide polymorphism rs6741949, in the DPP4 gene interacts with body adiposity and negatively affects glucose-stimulated GLP-1 levels, insulin secretion, and glucose tolerance.
Title: DPP4 gene variation affects GLP-1 secretion, insulin secretion, and glucose tolerance in humans with high body adiposity.

The following GCG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27494	NM_002054.5 Latest version!	Homo sapiens glucagon (GCG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu27494	NM_002054.4	Homo sapiens glucagon (GCG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCG ( NM_002054.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002045.1 Homo sapiens glucagon (GCG), mRNA.

Price & Availability↑

CloneID	OHu27494
Clone ID Related Accession (Same CDS sequence)	NM_002054.4 , NM_002054.5
Accession Version	NM_002054.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	glucagon preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM272442.1, BC005278.1 and CB069629.1. On Jun 1, 2012 this sequence version replaced NM_002054.3. Sequence Note: A downstream translational start codon is selected for this RefSeq based on transcript support and on the presence of a predicted signal peptide in the protein N-terminus. Several upstream in-frame start codons are also present with sparse transcript support, and would result in proteins that are up to 83 aa longer at the N-terminus and lack predicted signal peptides. Leaky scanning by ribosomes may allow translation initiation at the downstream start codon. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005278.1, J04040.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2156266 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal (PMID:3725587) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAAAGCA TTTACTTTGT GGCTGGATTA TTTGTAATGC TGGTACAAGG CAGCTGGCAA 
CGTTCCCTTC AAGACACAGA GGAGAAATCC AGATCATTCT CAGCTTCCCA GGCAGACCCA 
CTCAGTGATC CTGATCAGAT GAACGAGGAC AAGCGCCATT CACAGGGCAC ATTCACCAGT 
GACTACAGCA AGTATCTGGA CTCCAGGCGT GCCCAAGATT TTGTGCAGTG GTTGATGAAT 
ACCAAGAGGA ACAGGAATAA CATTGCCAAA CGTCACGATG AATTTGAGAG ACATGCTGAA 
GGGACCTTTA CCAGTGATGT AAGTTCTTAT TTGGAAGGCC AAGCTGCCAA GGAATTCATT 
GCTTGGCTGG TGAAAGGCCG AGGAAGGCGA GATTTCCCAG AAGAGGTCGC CATTGTTGAA 
GAACTTGGCC GCAGACATGC TGATGGTTCT TTCTCTGATG AGATGAACAC CATTCTTGAT 
AATCTTGCCG CCAGGGACTT TATAAACTGG TTGATTCAGA CCAAAATCAC TGACAGGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002045.1
CDS	257..799
Translation	MKSIYFVAGLFVMLVQGSWQRSLQDTEEKSRSFSASQADPLSDPDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNRNNIAKRHDEFERHAEGTFTSDVSSYLEGQAAKEFIAWLVKGRGRRDFPEEVAIVEELGRRHADGSFSDEMNTILDNLAARDFINWLIQTKITDRK

Target ORF information:

RefSeq Version	NM_002054.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glucagon (GCG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002054.4

ORF Insert Sequence:

ATGAAAAGCA TTTACTTTGT GGCTGGATTA TTTGTAATGC TGGTACAAGG CAGCTGGCAA 
CGTTCCCTTC AAGACACAGA GGAGAAATCC AGATCATTCT CAGCTTCCCA GGCAGACCCA 
CTCAGTGATC CTGATCAGAT GAACGAGGAC AAGCGCCATT CACAGGGCAC ATTCACCAGT 
GACTACAGCA AGTATCTGGA CTCCAGGCGT GCCCAAGATT TTGTGCAGTG GTTGATGAAT 
ACCAAGAGGA ACAGGAATAA CATTGCCAAA CGTCACGATG AATTTGAGAG ACATGCTGAA 
GGGACCTTTA CCAGTGATGT AAGTTCTTAT TTGGAAGGCC AAGCTGCCAA GGAATTCATT 
GCTTGGCTGG TGAAAGGCCG AGGAAGGCGA GATTTCCCAG AAGAGGTCGC CATTGTTGAA 
GAACTTGGCC GCAGACATGC TGATGGTTCT TTCTCTGATG AGATGAACAC CATTCTTGAT 
AATCTTGCCG CCAGGGACTT TATAAACTGG TTGATTCAGA CCAAAATCAC TGACAGGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCG ( NM_002054.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002045.1 Homo sapiens glucagon (GCG), mRNA.

Price & Availability↑

CloneID	OHu27494
Clone ID Related Accession (Same CDS sequence)	NM_002054.4 , NM_002054.5
Accession Version	NM_002054.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	glucagon preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005278.1 and CB069629.1. On Nov 24, 2018 this sequence version replaced NM_002054.4. Sequence Note: A downstream translational start codon is selected for this RefSeq based on transcript support and on the presence of a predicted signal peptide in the protein N-terminus. Several upstream in-frame start codons are also present with sparse transcript support, and would result in proteins that are up to 83 aa longer at the N-terminus and lack predicted signal peptides. Leaky scanning by ribosomes may allow translation initiation at the downstream start codon. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005278.1, BM479329.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2156266 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000418842.7/ ENSP00000387662.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAAAGCA TTTACTTTGT GGCTGGATTA TTTGTAATGC TGGTACAAGG CAGCTGGCAA 
CGTTCCCTTC AAGACACAGA GGAGAAATCC AGATCATTCT CAGCTTCCCA GGCAGACCCA 
CTCAGTGATC CTGATCAGAT GAACGAGGAC AAGCGCCATT CACAGGGCAC ATTCACCAGT 
GACTACAGCA AGTATCTGGA CTCCAGGCGT GCCCAAGATT TTGTGCAGTG GTTGATGAAT 
ACCAAGAGGA ACAGGAATAA CATTGCCAAA CGTCACGATG AATTTGAGAG ACATGCTGAA 
GGGACCTTTA CCAGTGATGT AAGTTCTTAT TTGGAAGGCC AAGCTGCCAA GGAATTCATT 
GCTTGGCTGG TGAAAGGCCG AGGAAGGCGA GATTTCCCAG AAGAGGTCGC CATTGTTGAA 
GAACTTGGCC GCAGACATGC TGATGGTTCT TTCTCTGATG AGATGAACAC CATTCTTGAT 
AATCTTGCCG CCAGGGACTT TATAAACTGG TTGATTCAGA CCAAAATCAC TGACAGGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002045.1
CDS	100..642
Translation	MKSIYFVAGLFVMLVQGSWQRSLQDTEEKSRSFSASQADPLSDPDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNRNNIAKRHDEFERHAEGTFTSDVSSYLEGQAAKEFIAWLVKGRGRRDFPEEVAIVEELGRRHADGSFSDEMNTILDNLAARDFINWLIQTKITDRK

Target ORF information:

RefSeq Version	NM_002054.5
Organism	Homo sapiens(human)
Definition	Homo sapiens glucagon (GCG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002054.5

ORF Insert Sequence:

ATGAAAAGCA TTTACTTTGT GGCTGGATTA TTTGTAATGC TGGTACAAGG CAGCTGGCAA 
CGTTCCCTTC AAGACACAGA GGAGAAATCC AGATCATTCT CAGCTTCCCA GGCAGACCCA 
CTCAGTGATC CTGATCAGAT GAACGAGGAC AAGCGCCATT CACAGGGCAC ATTCACCAGT 
GACTACAGCA AGTATCTGGA CTCCAGGCGT GCCCAAGATT TTGTGCAGTG GTTGATGAAT 
ACCAAGAGGA ACAGGAATAA CATTGCCAAA CGTCACGATG AATTTGAGAG ACATGCTGAA 
GGGACCTTTA CCAGTGATGT AAGTTCTTAT TTGGAAGGCC AAGCTGCCAA GGAATTCATT 
GCTTGGCTGG TGAAAGGCCG AGGAAGGCGA GATTTCCCAG AAGAGGTCGC CATTGTTGAA 
GAACTTGGCC GCAGACATGC TGATGGTTCT TTCTCTGATG AGATGAACAC CATTCTTGAT 
AATCTTGCCG CCAGGGACTT TATAAACTGG TTGATTCAGA CCAAAATCAC TGACAGGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GLP-1 response to sequential mixed meals: influence of insulin resistance.
Clinical science (London, England : 1979)131(24)2901-2910(2017 Dec)
Rebelos E,Astiarraga B,Bizzotto R,Mari A,Manca ML,Gonzalez A,Mendez A,Martinez CA,Hurwitz BE,Ferrannini E

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GCG cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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