ASPM cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASPM
Entrez Gene ID	259266
Full Name	abnormal spindle microtubule assembly
Synonyms	ASP,Calmbp1,MCPH5
General protein information	Preferred Names abnormal spindle microtubule assembly Names abnormal spindle-like microcephaly-associated protein asp (abnormal spindle) homolog, microcephaly associated
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q31.3
Summary	This gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts. Studies in mouse also suggest a role of this gene in mitotic spindle regulation, with a preferential role in regulating neurogenesis. Mutations in this gene are associated with microcephaly primary type 5. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011].
Disorder MIM:	605481
Disorder Html:	Microcephaly 5, primary, autosomal recessive, 608716 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_018136.4	NP_060606.3	abnormal spindle-like microcephaly-associated protein isoform 1
NM_001206846.1	NP_001193775.1	abnormal spindle-like microcephaly-associated protein isoform 2
NM_018136.5	NP_060606.3	abnormal spindle-like microcephaly-associated protein isoform 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Aspm	NP_001099425.2
Bos taurus (cattle)	ASPM	NP_001193435.1
Mus musculus (house mouse)	Aspm	NP_033921.3
Danio rerio (zebrafish)	aspm	NP_001116845.1
Pan troglodytes (chimpanzee)	ASPM	NP_001008994.1
Gallus gallus (chicken)	ASPM	XP_422197.4
Xenopus tropicalis (tropical clawed frog)	aspm	XP_002932224.2
Homo sapiens (human)	ASPM	NP_060606.3
	ASPM	NP_001098005.1
Canis lupus familiaris (dog)	ASPM	NP_001184005.1

Gene Ontology
Bibliography

Related articles in PubMed

Autosomal recessive primary microcephaly due to ASPM mutations: An update.
L?tard P, Drunat S, Vial Y, Duerinckx S, Ernault A, Amram D, Arpin S, Bertoli M, Busa T, Ceulemans B, Desir J, Doco-Fenzy M, Elalaoui SC, Devriendt K, Faivre L, Francannet C, Genevi?ve D, G?rard M, Gitiaux C, Julia S, Lebon S, Lubala T, Mathieu-Dramard M, Maurey H, Metreau J, Nasserereddine S, Nizon M, Pierquin G, Pouvreau N, Rivier-Ringenbach C, Rossi M, Schaefer E, Sefiani A, Sigaudy S, Sznajer Y, Tunca Y, Guilmin Crepon S, Alberti C, Elmaleh-Berg?s M, Benzacken B, Wollnick B, Woods CG, Rauch A, Abramowicz M, El Ghouzzi V, Gressens P, Verloes A, Passemard S
Human mutation(2017 Dec)

Recapitulating cortical development with organoid culture in vitro and modeling abnormal spindle-like (ASPM related primary) microcephaly disease.
Li R, Sun L, Fang A, Li P, Wu Q, Wang X
Protein & cell8(11)823-833(2017 Nov)

Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2.
Tungadi EA, Ito A, Kiyomitsu T, Goshima G
Journal of cell science130(21)3676-3684(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Abnormal cortical development in primary microcephaly was recapitulated with organoid culture in vitro using patient's induced pluripotent stem cells with ASPM mutation.
Title: Recapitulating cortical development with organoid culture in vitro and modeling abnormal spindle-like (ASPM related primary) microcephaly disease.

ASPM-katanin complex controls microtubule disassembly at spindle poles and that misregulation of this process can lead to microcephaly.
Title: Microtubule minus-end regulation at spindle poles by an ASPM-katanin complex.

The results confirm that mutations in ASPM or WDR62 are the major cause of autosomal recessive primary microcephaly in the Pakistani population.
Title: Molecular analysis of 23 Pakistani families with autosomal recessive primary microcephaly using targeted next-generation sequencing.

Through co-expression analysis, ASPM was identified and validated in association with the progression of Hepatitis C virus cirrhosis probably by regulating tumor-related phosphorylation.
Title: Strong correlation between ASPM gene expression and HCV cirrhosis progression identified by co-expression analysis.

A novel homozygous splice-site variant in the ASPM gene was identified. The variant is predicted to have an effect on splicing. Human Splice Finder, an in silico tool, predicted skipping of exon 16 due to this variant. Skipping of exon 16 may change the order and number of IQ motifs in the ASPM protein leading to typical autosomal recessive primary microcephaly phenotype.
Title: A novel splice-site mutation in the ASPM gene underlies autosomal recessive primary microcephaly.

The following ASPM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASPM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18894	NM_018136.5 Latest version!	Homo sapiens assembly factor for spindle microtubules (ASPM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu23051	NM_001206846.1 Latest version!	Homo sapiens assembly factor for spindle microtubules (ASPM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OHu18894	NM_018136.4	Homo sapiens abnormal spindle microtubule assembly (ASPM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASPM ( NM_018136.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060606.3 Homo sapiens abnormal spindle microtubule assembly (ASPM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18894
Clone ID Related Accession (Same CDS sequence)	NM_018136.4 , NM_018136.5
Accession Version	NM_018136.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Homo sapiens(human)
Product	abnormal spindle-like microcephaly-associated protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK125107.1, AF509326.1, AL353809.20, BC034607.1 and BX648804.1. This sequence is a reference standard in the RefSeqGene project. On Jul 15, 2008 this sequence version replaced NM_018136.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF509326.1, AY367065.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAACC GGCGAGTGGG GCGAGGCTGC TGGGAAGTGA GCCCGACCGA GCGGAGGCCG 
CCCGCGGGGC TGCGGGGCCC CGCGGCCGAG GAGGAGGCGT CTTCCCCGCC GGTCCTGTCT 
CTCAGCCACT TCTGCAGGTC TCCTTTCCTT TGCTTCGGGG ACGTTCTCCT GGGAGCCTCA 
CGGACGCTGT CTCTGGCCCT AGACAACCCT AACGAGGAGG TGGCAGAAGT GAAGATCTCC 
CACTTCCCGG CCGCGGACCT GGGCTTCAGT GTGTCGCAGC GCTGTTTCGT GTTGCAGCCT 
AAAGAGAAAA TTGTTATTTC TGTTAACTGG ACACCACTCA AAGAAGGCCG AGTAAGAGAG 
ATTATGACAT TTCTTGTAAA TGATGTTCTG AAACACCAAG CTATATTACT AGGAAATGCA 
GAAGAGCAGA AAAAGAAAAA GAGGAGTCTT TGGGATACCA TTAAAAAGAA GAAAATTTCA 
GCCTCTACAA GTCACAACAG AAGGGTTTCA AATATTCAGA ATGTTAATAA AACATTTAGT 
GTTTCCCAAA AAGTTGACAG AGTTAGGAGC CCACTACAAG CTTGTGAAAA CTTGGCTATG 
AATGAAGGCG GTCCCCCAAC AGAAAACAAT TCTTTAATAC TTGAAGAAAA TAAAATACCC 
ATATCACCTA TTAGCCCTGC TTTCAATGAA TGCCATGGTG CAACTTGCTT GCCACTCTCT 
GTACGTCGAT CTACTACCTA CTCATCTCTT CATGCATCAG AAAATAGGGA ACTATTAAAT 
GTACACAGTG CCAACGTTTC AAAAGTTTCT TTTAATGAGA AAGCTGTAAC TGAAACTTCC 
TTTAATTCCG TAAATGTTAA TGGCCAAAGA GGAGAGAATA GTAAACTTAG TCTTACCCCC 
AACTGTTCTT CAACTTTGAA CATTACACAA AGCCAAATAC ATTTTCTAAG TCCAGATTCT 
TTTGTAAATA ATAGTCATGG AGCTAATAAT GAACTAGAAT TAGTAACATG TCTTTCATCA 
GATATGTTTA TGAAAGATAA TTCACAGCCT GTGCATTTGG AATCAACAAT TGCACATGAA 
ATTTATCAGA AAATTTTAAG TCCAGATTCT TTCATAAAAG ATAATTATGG ACTAAATCAG 
GATCTAGAAT CAGAGTCAGT TAATCCTATT TTATCCCCTA ATCAATTTTT AAAAGATAAC 
ATGGCATATA TGTGTACATC TCAGCAAACA TGTAAAGTAC CATTATCAAA TGAAAATTCT 
CAAGTCCCAC AGTCTCCTGA AGATTGGAGA AAAAGTGAAG TTTCGCCACG TATTCCTGAA 
TGTCAGGGTT CAAAATCTCC CAAAGCTATT TTTGAAGAAC TAGTAGAAAT GAAGTCAAAT 
TACTACAGTT TTATAAAACA AAATAATCCT AAATTTTCTG CAGTTCAGGA TATTTCTAGT 
CATAGCCACA ATAAACAACC TAAGAGACGT CCAATACTTT CTGCCACTGT TACTAAAAGG 
AAGGCCACCT GTACCAGAGA AAACCAAACT GAGATTAATA AACCAAAAGC AAAAAGATGT 
CTCAACAGTG CAGTGGGTGA ACATGAAAAA GTAATAAATA ATCAAAAGGA AAAAGAAGAT 
TTTCATTCTT ATCTTCCAAT TATAGATCCA ATATTAAGTA AATCTAAGAG TTATAAAAAC 
GAGGTAACAC CCTCTTCGAC AACAGCTTCA GTTGCTCGGA AAAGAAAGAG CGATGGAAGC 
ATGGAAGATG CAAATGTGAG AGTTGCAATT ACAGAACATA CAGAAGTGCG AGAAATCAAA 
AGAATCCATT TTTCTCCCTC AGAGCCTAAA ACATCAGCTG TTAAGAAAAC AAAAAATGTG 
ACAACACCCA TCTCAAAACG TATTAGCAAC AGAGAGAAAT TAAACCTGAA GAAGAAAACT 
GATTTATCAA TATTCAGAAC TCCAATTTCT AAAACAAACA AAAGGACAAA ACCCATTATC 
GCTGTGGCAC AGTCCAGTTT GACCTTCATA AAACCATTAA AAACAGATAT TCCCAGACAC 
CCGATGCCAT TTGCTGCAAA AAACATGTTT TATGATGAAC GCTGGAAGGA AAAGCAGGAA 
CAGGGCTTCA CTTGGTGGTT AAATTTTATA TTAACCCCTG ATGACTTCAC TGTAAAAACA 
AATATTTCTG AAGTAAATGC TGCTACTCTT CTTTTGGGAA TAGAGAATCA ACATAAAATA 
AGTGTTCCTA GAGCACCTAC AAAAGAGGAA ATGTCTCTCA GAGCTTATAC TGCTCGGTGT 
AGGTTAAACA GACTACGTCG TGCAGCATGC CGTTTGTTTA CTTCTGAAAA AATGGTTAAA 
GCTATTAAAA AGCTTGAAAT TGAAATTGAA GCTAGGCGGT TAATTGTTCG AAAAGATAGA 
CACCTATGGA AAGATGTGGG AGAACGTCAG AAAGTCCTGA ATTGGCTGTT GTCCTACAAT 
CCTTTGTGGC TTCGAATTGG TCTAGAGACA ACTTATGGAG AACTCATATC TTTGGAAGAT 
AACAGTGATG TCACAGGGTT GGCTATGTTT ATTCTGAATC GCCTACTTTG GAATCCTGAT 
ATAGCAGCTG AGTATAGACA CCCCACTGTT CCTCACCTGT ATAGAGATGG TCATGAAGAA 
GCTTTGTCCA AGTTTACATT GAAAAAGTTA TTGTTGTTGG TCTGTTTTCT TGATTATGCT 
AAAATTTCCA GACTCATTGA TCATGATCCT TGTCTCTTCT GTAAAGATGC CGAATTCAAG 
GCTAGTAAAG AAATCCTTTT GGCTTTTTCA CGAGATTTCC TAAGTGGTGA AGGTGACCTT 
TCCCGTCACC TTGGCTTATT GGGATTACCT GTTAACCATG TTCAGACACC ATTTGATGAA 
TTTGATTTTG CCGTTACAAA TCTTGCCGTA GACTTGCAAT GTGGAGTGCG CCTTGTGCGA 
ACCATGGAAC TTCTCACACA GAACTGGGAC CTCTCAAAGA AACTCAGGAT TCCGGCAATA 
AGTCGTCTTC AAAAGATGCA CAATGTTGAC ATTGTTCTTC AAGTTCTTAA ATCACGAGGA 
ATTGAATTAA GTGATGAGCA TGGAAATACA ATTCTATCTA AGGATATTGT GGATAGGCAC 
AGAGAAAAAA CTCTCAGGTT GCTTTGGAAA ATAGCGTTTG CTTTTCAGGT GGATATTTCC 
CTTAACTTAG ATCAATTAAA GGAAGAAATT GCCTTTCTAA AACACACAAA GAGTATAAAG 
AAAACAATAT CTCTACTATC ATGCCATTCT GATGATCTTA TTAATAAGAA AAAAGGCAAA 
AGGGATAGTG GTTCCTTTGA ACAATATAGT GAAAACATAA AGTTATTGAT GGATTGGGTA 
AATGCTGTTT GTGCCTTCTA TAATAAAAAG GTGGAGAATT TTACAGTGTC TTTCTCAGAC 
GGCCGTGTGT TATGTTACCT GATCCACCAT TACCATCCTT GCTATGTGCC ATTTGACGCT 
ATATGTCAGC GTACTACTCA AACTGTGGAA TGTACGCAAA CTGGTTCAGT GGTATTAAAT 
TCATCATCTG AATCTGATGA CAGTTCTCTG GATATGTCTC TTAAAGCATT TGATCATGAA 
AATACTTCAG AGCTATACAA AGAGCTCCTA GAAAATGAAA AGAAAAATTT TCACTTGGTT 
AGGTCTGCAG TTAGAGACCT TGGTGGAATA CCTGCTATGA TTAATCATTC AGATATGTCA 
AATACAATTC CAGATGAAAA GGTGGTTATT ACCTATTTGT CATTTCTTTG TGCAAGGCTT 
TTGGATCTTC GTAAAGAAAT AAGAGCTGCT CGACTCATAC AAACAACATG GAGAAAATAT 
AAACTAAAAA CAGATCTCAA ACGCCATCAG GAGAGAGAGA AAGCTGCAAG AATTATTCAA 
TTGGCTGTAA TCAATTTTCT AGCAAAACAA AGATTGAGAA AAAGAGTTAA TGCAGCACTC 
GTCATTCAGA AATATTGGCG AAGAGTCTTA GCACAGAGAA AATTATTAAT GTTAAAAAAG 
GAAAAGCTGG AAAAAGTTCA AAATAAAGCA GCATCACTTA TTCAGGGATA TTGGAGAAGA 
TATTCCACTA GACAAAGATT TCTGAAATTG AAATATTATT CAATCATCCT GCAATCTAGG 
ATAAGAATGA TAATTGCTGT TACATCTTAT AAACGATATC TTTGGGCTAC AGTTACAATT 
CAGAGGCATT GGCGTGCTTA TTTAAGAAGA AAACAAGATC AACAAAGATA TGAAATGCTA 
AAATCATCAA CTCTTATAAT CCAATCTATG TTCAGAAAAT GGAAGCAACG TAAAATGCAA 
TCACAAGTAA AAGCTACAGT AATATTGCAA AGAGCTTTTA GAGAATGGCA TTTAAGAAAA 
CAAGCTAAAG AAGAAAATTC TGCTATTATC ATACAATCAT GGTATAGAAT GCATAAAGAA 
TTACGGAAAT ATATTTATAT TAGATCTTGT GTTGTTATCA TTCAGAAAAG ATTTCGGTGC 
TTTCAAGCCC AAAAGTTATA TAAAAGAAGA AAAGAGTCCA TACTAACCAT CCAGAAGTAC 
TACAAAGCAT ATCTGAAAGG AAAGATTGAG CGCACCAACT ATTTGCAGAA ACGAGCTGCA 
GCCATTCAAT TACAAGCTGC TTTTAGGAGA CTGAAAGCTC ATAATTTATG TAGACAAATT 
AGAGCTGCTT GTGTTATTCA GTCATACTGG AGAATGAGAC AAGACAGAGT TCGATTTTTA 
AACCTTAAGA AGACTATTAT CAAATTTCAG GCACATGTAA GAAAACATCA ACAACGACAG 
AAATATAAGA AGATGAAGAA AGCAGCTGTT ATAATTCAGA CTCATTTCCG AGCTTATATT 
TTTGCCATGA AAGTTCTAGC ATCTTACCAG AAAACACGCT CTGCTGTCAT TGTGCTGCAG 
TCTGCATATA GAGGGATGCA AGCCAGGAAA ATGTATATTC ACATCCTCAC ATCTGTTATA 
AAGATTCAAT CATATTATCG TGCTTATGTT TCTAAAAAGG AATTTTTGAG CCTAAAAAAT 
GCTACAATAA AATTGCAGTC AACTGTTAAG ATGAAACAAA CACGTAAACA ATATTTGCAT 
TTAAGAGCAG CTGCACTATT TATCCAGCAA TGTTACCGTT CCAAAAAAAT AGCTGCACAA 
AAGAGAGAAG AGTATATGCA GATGCGGGAA TCTTGTATCA AACTGCAAGC ATTTGTTAGA 
GGATACCTTG TCCGAAAGCA GATGAGGTTA CAAAGAAAAG CTGTTATTTC ACTACAGTCT 
TATTTCAGAA TGAGAAAGGC TCGGCAGTAT TATCTGAAAA TGTATAAAGC AATTATTGTC 
ATTCAGAATT ACTATCATGC ATACAAAGCA CAGGTCAATC AGAGGAAGAA CTTCTTGCAA 
GTCAAAAAAG CAGCTACTTG CTTGCAAGCA GCTTACAGAG GTTATAAAGT ACGCCAGCTA 
ATCAAACAAC AATCTATAGC TGCTCTTAAA ATTCAGTCTG CTTTTAGAGG CTATAATAAA 
AGGGTAAAAT ATCAATCTGT GCTTCAATCT ATAATAAAGA TTCAGAGATG GTACAGGGCG 
TACAAGACTC TTCATGATAC AAGAACACAT TTTTTGAAGA CAAAGGCAGC TGTGATTTCC 
CTCCAGTCTG CTTATCGTGG CTGGAAGGTT CGGAAACAGA TTAGAAGGGA ACATCAAGCT 
GCCTTGAAGA TTCAGTCTGC TTTTAGAATG GCCAAGGCCC AGAAACAGTT TAGATTGTTT 
AAAACAGCAG CATTAGTCAT CCAGCAAAAT TTCAGAGCAT GGACTGCAGG AAGGAAGCAA 
TGTATGGAGT ATATTGAACT CCGTCATGCG GTACTGGTGC TTCAATCTAT GTGGAAGGGA 
AAAACACTGA GAAGACAGCT TCAAAGGCAA CATAAATGTG CTATCATCAT ACAGTCATAC 
TATAGAATGC ATGTGCAACA AAAGAAGTGG AAAATCATGA AAAAAGCTGC TCTTCTGATT 
CAAAAGTATT ATAGGGCTTA CAGTATTGGA AGAGAACAGA ATCATTTATA TTTGAAAACA 
AAAGCAGCTG TAGTAACTTT ACAGTCAGCT TATCGTGGTA TGAAAGTGAG AAAAAGAATA 
AAGGATTGCA ACAAAGCAGC AGTCACTATA CAGTCTAAAT ACAGAGCTTA CAAAACCAAA 
AAGAAATATG CAACCTATAG AGCTTCAGCT ATTATAATTC AGAGATGGTA TCGAGGTATT 
AAAATTACAA ACCATCAGCA TAAGGAGTAT CTTAATTTGA AGAAGACAGC AATTAAAATC 
CAATCTGTTT ATAGAGGTAT TAGAGTTAGA AGACATATTC AACACATGCA CAGGGCAGCC 
ACTTTTATTA AAGCCATGTT TAAAATGCAT CAGTCAAGAA TAAGTTACCA TACAATGAGA 
AAAGCAGCTA TTGTTATTCA AGTAAGATGT AGAGCATATT ATCAAGGTAA AATGCAGCGT 
GAAAAGTACC TGACAATTTT GAAAGCTGTT AAAGTCCTTC AGGCAAGTTT TAGAGGAGTA 
AGAGTTAGAC GGACTCTTAG AAAGATGCAG ACTGCAGCAA CACTCATTCA GTCAAACTAC 
AGAAGATACA GACAGCAAAC ATACTTTAAT AAGTTAAAGA AAATAACAAA AACAGTACAG 
CAAAGATACT GGGCAATGAA AGAAAGAAAC ATACAATTTC AAAGGTATAA CAAACTGAGG 
CATTCTGTAA TATACATTCA GGCTATTTTT AGGGGAAAGA AAGCTAGAAG ACATTTAAAA 
ATGATGCATA TAGCCGCAAC TCTCATTCAG AGGAGATTTA GAACTCTAAT GATGAGAAGA 
AGATTCCTCT CTCTCAAGAA AACTGCTATT TTGATTCAGA GAAAATATCG GGCACATCTT 
TGTACAAAGC ATCACTTACA GTTCCTTCAG GTACAAAATG CAGTTATTAA AATCCAGTCA 
TCATACAGAA GATGGATGAT AAGGAAAAGG ATGCGAGAGA TGCACAGGGC TGCTACTTTC 
ATCCAGTCTA CTTTCAGAAT GCACAGATTA CATATGAGAT ATCAGGCTTT GAAACAGGCC 
TCCGTTGTGA TCCAACAGCA ATACCAAGCA AATAGAGCTG CAAAACTGCA GAGGCAGCAT 
TATCTCAGAC AAAGACACTC TGCTGTGATC CTTCAGGCTG CATTCAGGGG TATGAAAACT 
AGAAGACATT TGAAGAGTAT GCATTCCTCT GCAACCCTTA TTCAGAGTAG GTTTAGATCA 
TTACTGGTGA GGAGAAGATT CATTTCCCTC AAAAAAGCTA CTATTTTTGT TCAGAGGAAA 
TATCGAGCCA CCATTTGTGC CAAACATAAA TTGTACCAAT TCTTGCACTT AAGAAAGGCA 
GCCATTACAA TACAGTCATC TTACAGAAGA CTGATGGTAA AGAAGAAGTT ACAAGAAATG 
CAAAGGGCTG CAGTTCTCAT TCAGGCTACT TTCAGGATGT ACAGAACATA TATTACATTT 
CAGACTTGGA AACATGCTTC AATTCTAATT CAGCAACATT ATCGAACATA TAGAGCTGCA 
AAATTACAAA GAGAAAATTA TATCAGACAA TGGCATTCTG CTGTGGTTAT TCAGGCTGCA 
TATAAAGGAA TGAAAGCAAG ACAACTTTTA AGGGAAAAAC ACAAAGCTTC TATCGTAATA 
CAAAGCACCT ACAGAATGTA TAGGCAGTAT TGTTTCTACC AAAAGCTTCA GTGGGCTACA 
AAAATCATAC AAGAAAAATA TAGAGCAAAT AAAAAGAAAC AGAAAGTATT TCAACACAAT 
GAACTTAAGA AAGAGACTTG TGTTCAGGCA GGTTTTCAGG ACATGAACAT AAAAAAACAG 
ATTCAGGAAC AGCACCAGGC TGCCATTATT ATTCAGAAGC ATTGTAAAGC CTTTAAAATA 
AGGAAGCATT ATCTCCACCT TAGAGCAACA GTAGTTTCTA TTCAAAGAAG ATACAGAAAA 
CTAACTGCAG TGCGTACCCA AGCAGTTATT TGTATACAGT CTTATTACAG AGGCTTTAAA 
GTACGAAAGG ATATTCAAAA TATGCACCGG GCTGCCACAC TAATTCAGTC ATTCTATCGA 
ATGCACAGGG CCAAAGTTGA TTATGAAACA AAGAAAACTG CAATTGTGGT TATACAGAAT 
TATTATAGGT TGTATGTTAG AGTAAAAACA GAAAGAAAAA ACTTTTTAGC AGTTCAGAAA 
TCTGTACGAA CTATTCAGGC TGCTTTTAGA GGCATGAAAG TTAGACAAAA ATTGAAAAAT 
GTATCAGAGG AAAAGATGGC AGCCATTGTT AACCAATCTG CACTCTGCTG TTACAGAAGT 
AAAACTCAGT ATGAAGCTGT TCAAAGTGAA GGTGTTATGA TTCAAGAGTG GTATAAAGCT 
TCTGGCCTTG CTTGTTCACA GGAAGCAGAG TATCATTCTC AAAGTAGGGC TGCAGTAACA 
ATTCAAAAAG CTTTTTGTAG AATGGTCACA AGAAAACTGG AAACACAGAA ATGTGCTGCC 
CTACGGATTC AGTTCTTCCT TCAGATGGCT GTGTATCGGA GAAGATTTGT TCAGCAGAAA 
AGAGCTGCTA TCACTTTACA GCATTATTTT AGGACGTGGC AAACCAGAAA ACAGTTTTTA 
CTATATAGAA AAGCAGCAGT GGTTTTACAA AATCACTACA GAGCATTTCT GTCTGCAAAA 
CATCAAAGAC AAGTCTATTT ACAGATCAGA AGCAGTGTTA TCATTATTCA AGCTAGAAGT 
AAAGGATTTA TACAGAAACG GAAGTTTCAG GAAATTAAAA ATAGCACCAT AAAAATTCAG 
GCTATGTGGA GGAGATATAG AGCCAAGAAA TATTTATGTA AAGTGAAAGC TGCCTGCAAG 
ATTCAAGCCT GGTATAGATG TTGGAGAGCA CACAAAGAAT ATCTAGCTAT ATTAAAAGCT 
GTTAAAATTA TTCAAGGTTG CTTCTATACC AAACTAGAGA GAACACGGTT TTTGAATGTG 
AGAGCATCAG CAATTATCAT TCAGAGAAAA TGGAGAGCTA TACTTCCTGC AAAGATAGCT 
CATGAACACT TCTTAATGAT AAAAAGACAT CGAGCTGCTT GTTTGATCCA AGCACATTAT 
AGAGGATATA AAGGAAGGCA GGTCTTTCTT CGGCAGAAAT CTGCTGCTTT GATCATACAA 
AAATATATAC GAGCCAGGGA GGCTGGAAAG CATGAAAGGA TAAAATATAT TGAATTTAAA 
AAATCTACAG TTATCCTACA AGCACTGGTG CGTGGTTGGC TAGTACGAAA AAGATTTTTA 
GAACAGAGAG CCAAAATTCG ACTTCTTCAC TTCACTGCAG CTGCATATTA TCACCTGAAT 
GCTGTTAGAA TTCAAAGAGC CTATAAACTT TACCTGGCTG TGAAGAATGC TAACAAGCAG 
GTTAATTCAG TCATCTGTAT TCAGAGATGG TTTCGAGCAA GATTACAAGA AAAGAGATTT 
ATTCAGAAAT ATCATAGCAT CAAAAAGATT GAGCATGAAG GTCAAGAATG TCTGAGCCAG 
CGAAATAGGG CTGCATCAGT AATACAGAAA GCAGTGCGCC ATTTTCTCCT CCGTAAAAAG 
CAGGAAAAAT TCACTAGTGG AATCATTAAA ATTCAGGCAT TATGGAGAGG CTATTCTTGG 
AGGAAGAAAA ATGATTGTAC AAAAATTAAA GCTATACGAC TAAGTCTTCA AGTTGTTAAT 
AGGGAGATTC GAGAAGAAAA CAAACTCTAC AAAAGAACTG CACTTGCACT TCATTACCTT 
TTGACATATA AGCACCTTTC TGCCATTCTT GAGGCCTTAA AACACCTAGA GGTAGTTACT 
AGATTGTCTC CACTTTGTTG TGAGAACATG GCCCAGAGTG GAGCAATTTC TAAAATATTT 
GTTTTGATCC GAAGTTGTAA TCGCAGTATT CCTTGTATGG AAGTCATCAG ATATGCTGTG 
CAAGTCTTGC TTAATGTATC TAAGTATGAG AAAACTACTT CAGCAGTTTA TGATGTAGAA 
AATTGTATAG ATATACTATT GGAGCTTTTG CAGATATACC GAGAAAAGCC TGGTAATAAA 
GTTGCAGACA AAGGCGGAAG CATTTTTACA AAAACTTGTT GTTTGTTGGC TATTTTACTG 
AAGACAACAA ATAGAGCCTC TGATGTACGA AGTAGGTCCA AAGTTGTTGA CCGTATTTAC 
AGTCTCTACA AACTTACAGC TCATAAACAT AAAATGAATA CTGAAAGAAT ACTTTACAAG 
CAAAAGAAGA ATTCTTCTAT AAGCATTCCT TTTATCCCAG AAACACCTGT AAGGACCAGA 
ATAGTTTCAA GACTTAAGCC AGATTGGGTT TTGAGAAGAG ATAACATGGA AGAAATCACA 
AATCCCCTGC AAGCTATTCA AATGGTGATG GATACGCTTG GCATTCCTTA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060606.3
CDS	258..10691
Translation	MANRRVGRGCWEVSPTERRPPAGLRGPAAEEEASSPPVLSLSHFCRSPFLCFGDVLLGASRTLSLALDNPNEEVAEVKISHFPAADLGFSVSQRCFVLQPKEKIVISVNWTPLKEGRVREIMTFLVNDVLKHQAILLGNAEEQKKKKRSLWDTIKKKKISASTSHNRRVSNIQNVNKTFSVSQKVDRVRSPLQACENLAMNEGGPPTENNSLILEENKIPISPISPAFNECHGATCLPLSVRRSTTYSSLHASENRELLNVHSANVSKVSFNEKAVTETSFNSVNVNGQRGENSKLSLTPNCSSTLNITQSQIHFLSPDSFVNNSHGANNELELVTCLSSDMFMKDNSQPVHLESTIAHEIYQKILSPDSFIKDNYGLNQDLESESVNPILSPNQFLKDNMAYMCTSQQTCKVPLSNENSQVPQSPEDWRKSEVSPRIPECQGSKSPKAIFEELVEMKSNYYSFIKQNNPKFSAVQDISSHSHNKQPKRRPILSATVTKRKATCTRENQTEINKPKAKRCLNSAVGEHEKVINNQKEKEDFHSYLPIIDPILSKSKSYKNEVTPSSTTASVARKRKSDGSMEDANVRVAITEHTEVREIKRIHFSPSEPKTSAVKKTKNVTTPISKRISNREKLNLKKKTDLSIFRTPISKTNKRTKPIIAVAQSSLTFIKPLKTDIPRHPMPFAAKNMFYDERWKEKQEQGFTWWLNFILTPDDFTVKTNISEVNAATLLLGIENQHKISVPRAPTKEEMSLRAYTARCRLNRLRRAACRLFTSEKMVKAIKKLEIEIEARRLIVRKDRHLWKDVGERQKVLNWLLSYNPLWLRIGLETTYGELISLEDNSDVTGLAMFILNRLLWNPDIAAEYRHPTVPHLYRDGHEEALSKFTLKKLLLLVCFLDYAKISRLIDHDPCLFCKDAEFKASKEILLAFSRDFLSGEGDLSRHLGLLGLPVNHVQTPFDEFDFAVTNLAVDLQCGVRLVRTMELLTQNWDLSKKLRIPAISRLQKMHNVDIVLQVLKSRGIELSDEHGNTILSKDIVDRHREKTLRLLWKIAFAFQVDISLNLDQLKEEIAFLKHTKSIKKTISLLSCHSDDLINKKKGKRDSGSFEQYSENIKLLMDWVNAVCAFYNKKVENFTVSFSDGRVLCYLIHHYHPCYVPFDAICQRTTQTVECTQTGSVVLNSSSESDDSSLDMSLKAFDHENTSELYKELLENEKKNFHLVRSAVRDLGGIPAMINHSDMSNTIPDEKVVITYLSFLCARLLDLRKEIRAARLIQTTWRKYKLKTDLKRHQEREKAARIIQLAVINFLAKQRLRKRVNAALVIQKYWRRVLAQRKLLMLKKEKLEKVQNKAASLIQGYWRRYSTRQRFLKLKYYSIILQSRIRMIIAVTSYKRYLWATVTIQRHWRAYLRRKQDQQRYEMLKSSTLIIQSMFRKWKQRKMQSQVKATVILQRAFREWHLRKQAKEENSAIIIQSWYRMHKELRKYIYIRSCVVIIQKRFRCFQAQKLYKRRKESILTIQKYYKAYLKGKIERTNYLQKRAAAIQLQAAFRRLKAHNLCRQIRAACVIQSYWRMRQDRVRFLNLKKTIIKFQAHVRKHQQRQKYKKMKKAAVIIQTHFRAYIFAMKVLASYQKTRSAVIVLQSAYRGMQARKMYIHILTSVIKIQSYYRAYVSKKEFLSLKNATIKLQSTVKMKQTRKQYLHLRAAALFIQQCYRSKKIAAQKREEYMQMRESCIKLQAFVRGYLVRKQMRLQRKAVISLQSYFRMRKARQYYLKMYKAIIVIQNYYHAYKAQVNQRKNFLQVKKAATCLQAAYRGYKVRQLIKQQSIAALKIQSAFRGYNKRVKYQSVLQSIIKIQRWYRAYKTLHDTRTHFLKTKAAVISLQSAYRGWKVRKQIRREHQAALKIQSAFRMAKAQKQFRLFKTAALVIQQNFRAWTAGRKQCMEYIELRHAVLVLQSMWKGKTLRRQLQRQHKCAIIIQSYYRMHVQQKKWKIMKKAALLIQKYYRAYSIGREQNHLYLKTKAAVVTLQSAYRGMKVRKRIKDCNKAAVTIQSKYRAYKTKKKYATYRASAIIIQRWYRGIKITNHQHKEYLNLKKTAIKIQSVYRGIRVRRHIQHMHRAATFIKAMFKMHQSRISYHTMRKAAIVIQVRCRAYYQGKMQREKYLTILKAVKVLQASFRGVRVRRTLRKMQTAATLIQSNYRRYRQQTYFNKLKKITKTVQQRYWAMKERNIQFQRYNKLRHSVIYIQAIFRGKKARRHLKMMHIAATLIQRRFRTLMMRRRFLSLKKTAILIQRKYRAHLCTKHHLQFLQVQNAVIKIQSSYRRWMIRKRMREMHRAATFIQSTFRMHRLHMRYQALKQASVVIQQQYQANRAAKLQRQHYLRQRHSAVILQAAFRGMKTRRHLKSMHSSATLIQSRFRSLLVRRRFISLKKATIFVQRKYRATICAKHKLYQFLHLRKAAITIQSSYRRLMVKKKLQEMQRAAVLIQATFRMYRTYITFQTWKHASILIQQHYRTYRAAKLQRENYIRQWHSAVVIQAAYKGMKARQLLREKHKASIVIQSTYRMYRQYCFYQKLQWATKIIQEKYRANKKKQKVFQHNELKKETCVQAGFQDMNIKKQIQEQHQAAIIIQKHCKAFKIRKHYLHLRATVVSIQRRYRKLTAVRTQAVICIQSYYRGFKVRKDIQNMHRAATLIQSFYRMHRAKVDYETKKTAIVVIQNYYRLYVRVKTERKNFLAVQKSVRTIQAAFRGMKVRQKLKNVSEEKMAAIVNQSALCCYRSKTQYEAVQSEGVMIQEWYKASGLACSQEAEYHSQSRAAVTIQKAFCRMVTRKLETQKCAALRIQFFLQMAVYRRRFVQQKRAAITLQHYFRTWQTRKQFLLYRKAAVVLQNHYRAFLSAKHQRQVYLQIRSSVIIIQARSKGFIQKRKFQEIKNSTIKIQAMWRRYRAKKYLCKVKAACKIQAWYRCWRAHKEYLAILKAVKIIQGCFYTKLERTRFLNVRASAIIIQRKWRAILPAKIAHEHFLMIKRHRAACLIQAHYRGYKGRQVFLRQKSAALIIQKYIRAREAGKHERIKYIEFKKSTVILQALVRGWLVRKRFLEQRAKIRLLHFTAAAYYHLNAVRIQRAYKLYLAVKNANKQVNSVICIQRWFRARLQEKRFIQKYHSIKKIEHEGQECLSQRNRAASVIQKAVRHFLLRKKQEKFTSGIIKIQALWRGYSWRKKNDCTKIKAIRLSLQVVNREIREENKLYKRTALALHYLLTYKHLSAILEALKHLEVVTRLSPLCCENMAQSGAISKIFVLIRSCNRSIPCMEVIRYAVQVLLNVSKYEKTTSAVYDVENCIDILLELLQIYREKPGNKVADKGGSIFTKTCCLLAILLKTTNRASDVRSRSKVVDRIYSLYKLTAHKHKMNTERILYKQKKNSSISIPFIPETPVRTRIVSRLKPDWVLRRDNMEEITNPLQAIQMVMDTLGIPY

Target ORF information:

RefSeq Version	NM_018136.4
Organism	Homo sapiens(human)
Definition	Homo sapiens abnormal spindle microtubule assembly (ASPM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018136.4

ORF Insert Sequence:

ATGGCGAACC GGCGAGTGGG GCGAGGCTGC TGGGAAGTGA GCCCGACCGA GCGGAGGCCG 
CCCGCGGGGC TGCGGGGCCC CGCGGCCGAG GAGGAGGCGT CTTCCCCGCC GGTCCTGTCT 
CTCAGCCACT TCTGCAGGTC TCCTTTCCTT TGCTTCGGGG ACGTTCTCCT GGGAGCCTCA 
CGGACGCTGT CTCTGGCCCT AGACAACCCT AACGAGGAGG TGGCAGAAGT GAAGATCTCC 
CACTTCCCGG CCGCGGACCT GGGCTTCAGT GTGTCGCAGC GCTGTTTCGT GTTGCAGCCT 
AAAGAGAAAA TTGTTATTTC TGTTAACTGG ACACCACTCA AAGAAGGCCG AGTAAGAGAG 
ATTATGACAT TTCTTGTAAA TGATGTTCTG AAACACCAAG CTATATTACT AGGAAATGCA 
GAAGAGCAGA AAAAGAAAAA GAGGAGTCTT TGGGATACCA TTAAAAAGAA GAAAATTTCA 
GCCTCTACAA GTCACAACAG AAGGGTTTCA AATATTCAGA ATGTTAATAA AACATTTAGT 
GTTTCCCAAA AAGTTGACAG AGTTAGGAGC CCACTACAAG CTTGTGAAAA CTTGGCTATG 
AATGAAGGCG GTCCCCCAAC AGAAAACAAT TCTTTAATAC TTGAAGAAAA TAAAATACCC 
ATATCACCTA TTAGCCCTGC TTTCAATGAA TGCCATGGTG CAACTTGCTT GCCACTCTCT 
GTACGTCGAT CTACTACCTA CTCATCTCTT CATGCATCAG AAAATAGGGA ACTATTAAAT 
GTACACAGTG CCAACGTTTC AAAAGTTTCT TTTAATGAGA AAGCTGTAAC TGAAACTTCC 
TTTAATTCCG TAAATGTTAA TGGCCAAAGA GGAGAGAATA GTAAACTTAG TCTTACCCCC 
AACTGTTCTT CAACTTTGAA CATTACACAA AGCCAAATAC ATTTTCTAAG TCCAGATTCT 
TTTGTAAATA ATAGTCATGG AGCTAATAAT GAACTAGAAT TAGTAACATG TCTTTCATCA 
GATATGTTTA TGAAAGATAA TTCACAGCCT GTGCATTTGG AATCAACAAT TGCACATGAA 
ATTTATCAGA AAATTTTAAG TCCAGATTCT TTCATAAAAG ATAATTATGG ACTAAATCAG 
GATCTAGAAT CAGAGTCAGT TAATCCTATT TTATCCCCTA ATCAATTTTT AAAAGATAAC 
ATGGCATATA TGTGTACATC TCAGCAAACA TGTAAAGTAC CATTATCAAA TGAAAATTCT 
CAAGTCCCAC AGTCTCCTGA AGATTGGAGA AAAAGTGAAG TTTCGCCACG TATTCCTGAA 
TGTCAGGGTT CAAAATCTCC CAAAGCTATT TTTGAAGAAC TAGTAGAAAT GAAGTCAAAT 
TACTACAGTT TTATAAAACA AAATAATCCT AAATTTTCTG CAGTTCAGGA TATTTCTAGT 
CATAGCCACA ATAAACAACC TAAGAGACGT CCAATACTTT CTGCCACTGT TACTAAAAGG 
AAGGCCACCT GTACCAGAGA AAACCAAACT GAGATTAATA AACCAAAAGC AAAAAGATGT 
CTCAACAGTG CAGTGGGTGA ACATGAAAAA GTAATAAATA ATCAAAAGGA AAAAGAAGAT 
TTTCATTCTT ATCTTCCAAT TATAGATCCA ATATTAAGTA AATCTAAGAG TTATAAAAAC 
GAGGTAACAC CCTCTTCGAC AACAGCTTCA GTTGCTCGGA AAAGAAAGAG CGATGGAAGC 
ATGGAAGATG CAAATGTGAG AGTTGCAATT ACAGAACATA CAGAAGTGCG AGAAATCAAA 
AGAATCCATT TTTCTCCCTC AGAGCCTAAA ACATCAGCTG TTAAGAAAAC AAAAAATGTG 
ACAACACCCA TCTCAAAACG TATTAGCAAC AGAGAGAAAT TAAACCTGAA GAAGAAAACT 
GATTTATCAA TATTCAGAAC TCCAATTTCT AAAACAAACA AAAGGACAAA ACCCATTATC 
GCTGTGGCAC AGTCCAGTTT GACCTTCATA AAACCATTAA AAACAGATAT TCCCAGACAC 
CCGATGCCAT TTGCTGCAAA AAACATGTTT TATGATGAAC GCTGGAAGGA AAAGCAGGAA 
CAGGGCTTCA CTTGGTGGTT AAATTTTATA TTAACCCCTG ATGACTTCAC TGTAAAAACA 
AATATTTCTG AAGTAAATGC TGCTACTCTT CTTTTGGGAA TAGAGAATCA ACATAAAATA 
AGTGTTCCTA GAGCACCTAC AAAAGAGGAA ATGTCTCTCA GAGCTTATAC TGCTCGGTGT 
AGGTTAAACA GACTACGTCG TGCAGCATGC CGTTTGTTTA CTTCTGAAAA AATGGTTAAA 
GCTATTAAAA AGCTTGAAAT TGAAATTGAA GCTAGGCGGT TAATTGTTCG AAAAGATAGA 
CACCTATGGA AAGATGTGGG AGAACGTCAG AAAGTCCTGA ATTGGCTGTT GTCCTACAAT 
CCTTTGTGGC TTCGAATTGG TCTAGAGACA ACTTATGGAG AACTCATATC TTTGGAAGAT 
AACAGTGATG TCACAGGGTT GGCTATGTTT ATTCTGAATC GCCTACTTTG GAATCCTGAT 
ATAGCAGCTG AGTATAGACA CCCCACTGTT CCTCACCTGT ATAGAGATGG TCATGAAGAA 
GCTTTGTCCA AGTTTACATT GAAAAAGTTA TTGTTGTTGG TCTGTTTTCT TGATTATGCT 
AAAATTTCCA GACTCATTGA TCATGATCCT TGTCTCTTCT GTAAAGATGC CGAATTCAAG 
GCTAGTAAAG AAATCCTTTT GGCTTTTTCA CGAGATTTCC TAAGTGGTGA AGGTGACCTT 
TCCCGTCACC TTGGCTTATT GGGATTACCT GTTAACCATG TTCAGACACC ATTTGATGAA 
TTTGATTTTG CCGTTACAAA TCTTGCCGTA GACTTGCAAT GTGGAGTGCG CCTTGTGCGA 
ACCATGGAAC TTCTCACACA GAACTGGGAC CTCTCAAAGA AACTCAGGAT TCCGGCAATA 
AGTCGTCTTC AAAAGATGCA CAATGTTGAC ATTGTTCTTC AAGTTCTTAA ATCACGAGGA 
ATTGAATTAA GTGATGAGCA TGGAAATACA ATTCTATCTA AGGATATTGT GGATAGGCAC 
AGAGAAAAAA CTCTCAGGTT GCTTTGGAAA ATAGCGTTTG CTTTTCAGGT GGATATTTCC 
CTTAACTTAG ATCAATTAAA GGAAGAAATT GCCTTTCTAA AACACACAAA GAGTATAAAG 
AAAACAATAT CTCTACTATC ATGCCATTCT GATGATCTTA TTAATAAGAA AAAAGGCAAA 
AGGGATAGTG GTTCCTTTGA ACAATATAGT GAAAACATAA AGTTATTGAT GGATTGGGTA 
AATGCTGTTT GTGCCTTCTA TAATAAAAAG GTGGAGAATT TTACAGTGTC TTTCTCAGAC 
GGCCGTGTGT TATGTTACCT GATCCACCAT TACCATCCTT GCTATGTGCC ATTTGACGCT 
ATATGTCAGC GTACTACTCA AACTGTGGAA TGTACGCAAA CTGGTTCAGT GGTATTAAAT 
TCATCATCTG AATCTGATGA CAGTTCTCTG GATATGTCTC TTAAAGCATT TGATCATGAA 
AATACTTCAG AGCTATACAA AGAGCTCCTA GAAAATGAAA AGAAAAATTT TCACTTGGTT 
AGGTCTGCAG TTAGAGACCT TGGTGGAATA CCTGCTATGA TTAATCATTC AGATATGTCA 
AATACAATTC CAGATGAAAA GGTGGTTATT ACCTATTTGT CATTTCTTTG TGCAAGGCTT 
TTGGATCTTC GTAAAGAAAT AAGAGCTGCT CGACTCATAC AAACAACATG GAGAAAATAT 
AAACTAAAAA CAGATCTCAA ACGCCATCAG GAGAGAGAGA AAGCTGCAAG AATTATTCAA 
TTGGCTGTAA TCAATTTTCT AGCAAAACAA AGATTGAGAA AAAGAGTTAA TGCAGCACTC 
GTCATTCAGA AATATTGGCG AAGAGTCTTA GCACAGAGAA AATTATTAAT GTTAAAAAAG 
GAAAAGCTGG AAAAAGTTCA AAATAAAGCA GCATCACTTA TTCAGGGATA TTGGAGAAGA 
TATTCCACTA GACAAAGATT TCTGAAATTG AAATATTATT CAATCATCCT GCAATCTAGG 
ATAAGAATGA TAATTGCTGT TACATCTTAT AAACGATATC TTTGGGCTAC AGTTACAATT 
CAGAGGCATT GGCGTGCTTA TTTAAGAAGA AAACAAGATC AACAAAGATA TGAAATGCTA 
AAATCATCAA CTCTTATAAT CCAATCTATG TTCAGAAAAT GGAAGCAACG TAAAATGCAA 
TCACAAGTAA AAGCTACAGT AATATTGCAA AGAGCTTTTA GAGAATGGCA TTTAAGAAAA 
CAAGCTAAAG AAGAAAATTC TGCTATTATC ATACAATCAT GGTATAGAAT GCATAAAGAA 
TTACGGAAAT ATATTTATAT TAGATCTTGT GTTGTTATCA TTCAGAAAAG ATTTCGGTGC 
TTTCAAGCCC AAAAGTTATA TAAAAGAAGA AAAGAGTCCA TACTAACCAT CCAGAAGTAC 
TACAAAGCAT ATCTGAAAGG AAAGATTGAG CGCACCAACT ATTTGCAGAA ACGAGCTGCA 
GCCATTCAAT TACAAGCTGC TTTTAGGAGA CTGAAAGCTC ATAATTTATG TAGACAAATT 
AGAGCTGCTT GTGTTATTCA GTCATACTGG AGAATGAGAC AAGACAGAGT TCGATTTTTA 
AACCTTAAGA AGACTATTAT CAAATTTCAG GCACATGTAA GAAAACATCA ACAACGACAG 
AAATATAAGA AGATGAAGAA AGCAGCTGTT ATAATTCAGA CTCATTTCCG AGCTTATATT 
TTTGCCATGA AAGTTCTAGC ATCTTACCAG AAAACACGCT CTGCTGTCAT TGTGCTGCAG 
TCTGCATATA GAGGGATGCA AGCCAGGAAA ATGTATATTC ACATCCTCAC ATCTGTTATA 
AAGATTCAAT CATATTATCG TGCTTATGTT TCTAAAAAGG AATTTTTGAG CCTAAAAAAT 
GCTACAATAA AATTGCAGTC AACTGTTAAG ATGAAACAAA CACGTAAACA ATATTTGCAT 
TTAAGAGCAG CTGCACTATT TATCCAGCAA TGTTACCGTT CCAAAAAAAT AGCTGCACAA 
AAGAGAGAAG AGTATATGCA GATGCGGGAA TCTTGTATCA AACTGCAAGC ATTTGTTAGA 
GGATACCTTG TCCGAAAGCA GATGAGGTTA CAAAGAAAAG CTGTTATTTC ACTACAGTCT 
TATTTCAGAA TGAGAAAGGC TCGGCAGTAT TATCTGAAAA TGTATAAAGC AATTATTGTC 
ATTCAGAATT ACTATCATGC ATACAAAGCA CAGGTCAATC AGAGGAAGAA CTTCTTGCAA 
GTCAAAAAAG CAGCTACTTG CTTGCAAGCA GCTTACAGAG GTTATAAAGT ACGCCAGCTA 
ATCAAACAAC AATCTATAGC TGCTCTTAAA ATTCAGTCTG CTTTTAGAGG CTATAATAAA 
AGGGTAAAAT ATCAATCTGT GCTTCAATCT ATAATAAAGA TTCAGAGATG GTACAGGGCG 
TACAAGACTC TTCATGATAC AAGAACACAT TTTTTGAAGA CAAAGGCAGC TGTGATTTCC 
CTCCAGTCTG CTTATCGTGG CTGGAAGGTT CGGAAACAGA TTAGAAGGGA ACATCAAGCT 
GCCTTGAAGA TTCAGTCTGC TTTTAGAATG GCCAAGGCCC AGAAACAGTT TAGATTGTTT 
AAAACAGCAG CATTAGTCAT CCAGCAAAAT TTCAGAGCAT GGACTGCAGG AAGGAAGCAA 
TGTATGGAGT ATATTGAACT CCGTCATGCG GTACTGGTGC TTCAATCTAT GTGGAAGGGA 
AAAACACTGA GAAGACAGCT TCAAAGGCAA CATAAATGTG CTATCATCAT ACAGTCATAC 
TATAGAATGC ATGTGCAACA AAAGAAGTGG AAAATCATGA AAAAAGCTGC TCTTCTGATT 
CAAAAGTATT ATAGGGCTTA CAGTATTGGA AGAGAACAGA ATCATTTATA TTTGAAAACA 
AAAGCAGCTG TAGTAACTTT ACAGTCAGCT TATCGTGGTA TGAAAGTGAG AAAAAGAATA 
AAGGATTGCA ACAAAGCAGC AGTCACTATA CAGTCTAAAT ACAGAGCTTA CAAAACCAAA 
AAGAAATATG CAACCTATAG AGCTTCAGCT ATTATAATTC AGAGATGGTA TCGAGGTATT 
AAAATTACAA ACCATCAGCA TAAGGAGTAT CTTAATTTGA AGAAGACAGC AATTAAAATC 
CAATCTGTTT ATAGAGGTAT TAGAGTTAGA AGACATATTC AACACATGCA CAGGGCAGCC 
ACTTTTATTA AAGCCATGTT TAAAATGCAT CAGTCAAGAA TAAGTTACCA TACAATGAGA 
AAAGCAGCTA TTGTTATTCA AGTAAGATGT AGAGCATATT ATCAAGGTAA AATGCAGCGT 
GAAAAGTACC TGACAATTTT GAAAGCTGTT AAAGTCCTTC AGGCAAGTTT TAGAGGAGTA 
AGAGTTAGAC GGACTCTTAG AAAGATGCAG ACTGCAGCAA CACTCATTCA GTCAAACTAC 
AGAAGATACA GACAGCAAAC ATACTTTAAT AAGTTAAAGA AAATAACAAA AACAGTACAG 
CAAAGATACT GGGCAATGAA AGAAAGAAAC ATACAATTTC AAAGGTATAA CAAACTGAGG 
CATTCTGTAA TATACATTCA GGCTATTTTT AGGGGAAAGA AAGCTAGAAG ACATTTAAAA 
ATGATGCATA TAGCCGCAAC TCTCATTCAG AGGAGATTTA GAACTCTAAT GATGAGAAGA 
AGATTCCTCT CTCTCAAGAA AACTGCTATT TTGATTCAGA GAAAATATCG GGCACATCTT 
TGTACAAAGC ATCACTTACA GTTCCTTCAG GTACAAAATG CAGTTATTAA AATCCAGTCA 
TCATACAGAA GATGGATGAT AAGGAAAAGG ATGCGAGAGA TGCACAGGGC TGCTACTTTC 
ATCCAGTCTA CTTTCAGAAT GCACAGATTA CATATGAGAT ATCAGGCTTT GAAACAGGCC 
TCCGTTGTGA TCCAACAGCA ATACCAAGCA AATAGAGCTG CAAAACTGCA GAGGCAGCAT 
TATCTCAGAC AAAGACACTC TGCTGTGATC CTTCAGGCTG CATTCAGGGG TATGAAAACT 
AGAAGACATT TGAAGAGTAT GCATTCCTCT GCAACCCTTA TTCAGAGTAG GTTTAGATCA 
TTACTGGTGA GGAGAAGATT CATTTCCCTC AAAAAAGCTA CTATTTTTGT TCAGAGGAAA 
TATCGAGCCA CCATTTGTGC CAAACATAAA TTGTACCAAT TCTTGCACTT AAGAAAGGCA 
GCCATTACAA TACAGTCATC TTACAGAAGA CTGATGGTAA AGAAGAAGTT ACAAGAAATG 
CAAAGGGCTG CAGTTCTCAT TCAGGCTACT TTCAGGATGT ACAGAACATA TATTACATTT 
CAGACTTGGA AACATGCTTC AATTCTAATT CAGCAACATT ATCGAACATA TAGAGCTGCA 
AAATTACAAA GAGAAAATTA TATCAGACAA TGGCATTCTG CTGTGGTTAT TCAGGCTGCA 
TATAAAGGAA TGAAAGCAAG ACAACTTTTA AGGGAAAAAC ACAAAGCTTC TATCGTAATA 
CAAAGCACCT ACAGAATGTA TAGGCAGTAT TGTTTCTACC AAAAGCTTCA GTGGGCTACA 
AAAATCATAC AAGAAAAATA TAGAGCAAAT AAAAAGAAAC AGAAAGTATT TCAACACAAT 
GAACTTAAGA AAGAGACTTG TGTTCAGGCA GGTTTTCAGG ACATGAACAT AAAAAAACAG 
ATTCAGGAAC AGCACCAGGC TGCCATTATT ATTCAGAAGC ATTGTAAAGC CTTTAAAATA 
AGGAAGCATT ATCTCCACCT TAGAGCAACA GTAGTTTCTA TTCAAAGAAG ATACAGAAAA 
CTAACTGCAG TGCGTACCCA AGCAGTTATT TGTATACAGT CTTATTACAG AGGCTTTAAA 
GTACGAAAGG ATATTCAAAA TATGCACCGG GCTGCCACAC TAATTCAGTC ATTCTATCGA 
ATGCACAGGG CCAAAGTTGA TTATGAAACA AAGAAAACTG CAATTGTGGT TATACAGAAT 
TATTATAGGT TGTATGTTAG AGTAAAAACA GAAAGAAAAA ACTTTTTAGC AGTTCAGAAA 
TCTGTACGAA CTATTCAGGC TGCTTTTAGA GGCATGAAAG TTAGACAAAA ATTGAAAAAT 
GTATCAGAGG AAAAGATGGC AGCCATTGTT AACCAATCTG CACTCTGCTG TTACAGAAGT 
AAAACTCAGT ATGAAGCTGT TCAAAGTGAA GGTGTTATGA TTCAAGAGTG GTATAAAGCT 
TCTGGCCTTG CTTGTTCACA GGAAGCAGAG TATCATTCTC AAAGTAGGGC TGCAGTAACA 
ATTCAAAAAG CTTTTTGTAG AATGGTCACA AGAAAACTGG AAACACAGAA ATGTGCTGCC 
CTACGGATTC AGTTCTTCCT TCAGATGGCT GTGTATCGGA GAAGATTTGT TCAGCAGAAA 
AGAGCTGCTA TCACTTTACA GCATTATTTT AGGACGTGGC AAACCAGAAA ACAGTTTTTA 
CTATATAGAA AAGCAGCAGT GGTTTTACAA AATCACTACA GAGCATTTCT GTCTGCAAAA 
CATCAAAGAC AAGTCTATTT ACAGATCAGA AGCAGTGTTA TCATTATTCA AGCTAGAAGT 
AAAGGATTTA TACAGAAACG GAAGTTTCAG GAAATTAAAA ATAGCACCAT AAAAATTCAG 
GCTATGTGGA GGAGATATAG AGCCAAGAAA TATTTATGTA AAGTGAAAGC TGCCTGCAAG 
ATTCAAGCCT GGTATAGATG TTGGAGAGCA CACAAAGAAT ATCTAGCTAT ATTAAAAGCT 
GTTAAAATTA TTCAAGGTTG CTTCTATACC AAACTAGAGA GAACACGGTT TTTGAATGTG 
AGAGCATCAG CAATTATCAT TCAGAGAAAA TGGAGAGCTA TACTTCCTGC AAAGATAGCT 
CATGAACACT TCTTAATGAT AAAAAGACAT CGAGCTGCTT GTTTGATCCA AGCACATTAT 
AGAGGATATA AAGGAAGGCA GGTCTTTCTT CGGCAGAAAT CTGCTGCTTT GATCATACAA 
AAATATATAC GAGCCAGGGA GGCTGGAAAG CATGAAAGGA TAAAATATAT TGAATTTAAA 
AAATCTACAG TTATCCTACA AGCACTGGTG CGTGGTTGGC TAGTACGAAA AAGATTTTTA 
GAACAGAGAG CCAAAATTCG ACTTCTTCAC TTCACTGCAG CTGCATATTA TCACCTGAAT 
GCTGTTAGAA TTCAAAGAGC CTATAAACTT TACCTGGCTG TGAAGAATGC TAACAAGCAG 
GTTAATTCAG TCATCTGTAT TCAGAGATGG TTTCGAGCAA GATTACAAGA AAAGAGATTT 
ATTCAGAAAT ATCATAGCAT CAAAAAGATT GAGCATGAAG GTCAAGAATG TCTGAGCCAG 
CGAAATAGGG CTGCATCAGT AATACAGAAA GCAGTGCGCC ATTTTCTCCT CCGTAAAAAG 
CAGGAAAAAT TCACTAGTGG AATCATTAAA ATTCAGGCAT TATGGAGAGG CTATTCTTGG 
AGGAAGAAAA ATGATTGTAC AAAAATTAAA GCTATACGAC TAAGTCTTCA AGTTGTTAAT 
AGGGAGATTC GAGAAGAAAA CAAACTCTAC AAAAGAACTG CACTTGCACT TCATTACCTT 
TTGACATATA AGCACCTTTC TGCCATTCTT GAGGCCTTAA AACACCTAGA GGTAGTTACT 
AGATTGTCTC CACTTTGTTG TGAGAACATG GCCCAGAGTG GAGCAATTTC TAAAATATTT 
GTTTTGATCC GAAGTTGTAA TCGCAGTATT CCTTGTATGG AAGTCATCAG ATATGCTGTG 
CAAGTCTTGC TTAATGTATC TAAGTATGAG AAAACTACTT CAGCAGTTTA TGATGTAGAA 
AATTGTATAG ATATACTATT GGAGCTTTTG CAGATATACC GAGAAAAGCC TGGTAATAAA 
GTTGCAGACA AAGGCGGAAG CATTTTTACA AAAACTTGTT GTTTGTTGGC TATTTTACTG 
AAGACAACAA ATAGAGCCTC TGATGTACGA AGTAGGTCCA AAGTTGTTGA CCGTATTTAC 
AGTCTCTACA AACTTACAGC TCATAAACAT AAAATGAATA CTGAAAGAAT ACTTTACAAG 
CAAAAGAAGA ATTCTTCTAT AAGCATTCCT TTTATCCCAG AAACACCTGT AAGGACCAGA 
ATAGTTTCAA GACTTAAGCC AGATTGGGTT TTGAGAAGAG ATAACATGGA AGAAATCACA 
AATCCCCTGC AAGCTATTCA AATGGTGATG GATACGCTTG GCATTCCTTA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASPM ( NM_018136.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060606.3 Homo sapiens assembly factor for spindle microtubules (ASPM), transcript variant 1, mRNA.

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CloneID	OHu18894
Clone ID Related Accession (Same CDS sequence)	NM_018136.4 , NM_018136.5
Accession Version	NM_018136.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Homo sapiens(human)
Product	abnormal spindle-like microcephaly-associated protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK125107.1, AF509326.1, AL353809.20, BC034607.1 and BX648804.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_018136.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF509326.1, AY367065.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000367409.9/ ENSP00000356379.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGAACC GGCGAGTGGG GCGAGGCTGC TGGGAAGTGA GCCCGACCGA GCGGAGGCCG 
CCCGCGGGGC TGCGGGGCCC CGCGGCCGAG GAGGAGGCGT CTTCCCCGCC GGTCCTGTCT 
CTCAGCCACT TCTGCAGGTC TCCTTTCCTT TGCTTCGGGG ACGTTCTCCT GGGAGCCTCA 
CGGACGCTGT CTCTGGCCCT AGACAACCCT AACGAGGAGG TGGCAGAAGT GAAGATCTCC 
CACTTCCCGG CCGCGGACCT GGGCTTCAGT GTGTCGCAGC GCTGTTTCGT GTTGCAGCCT 
AAAGAGAAAA TTGTTATTTC TGTTAACTGG ACACCACTCA AAGAAGGCCG AGTAAGAGAG 
ATTATGACAT TTCTTGTAAA TGATGTTCTG AAACACCAAG CTATATTACT AGGAAATGCA 
GAAGAGCAGA AAAAGAAAAA GAGGAGTCTT TGGGATACCA TTAAAAAGAA GAAAATTTCA 
GCCTCTACAA GTCACAACAG AAGGGTTTCA AATATTCAGA ATGTTAATAA AACATTTAGT 
GTTTCCCAAA AAGTTGACAG AGTTAGGAGC CCACTACAAG CTTGTGAAAA CTTGGCTATG 
AATGAAGGCG GTCCCCCAAC AGAAAACAAT TCTTTAATAC TTGAAGAAAA TAAAATACCC 
ATATCACCTA TTAGCCCTGC TTTCAATGAA TGCCATGGTG CAACTTGCTT GCCACTCTCT 
GTACGTCGAT CTACTACCTA CTCATCTCTT CATGCATCAG AAAATAGGGA ACTATTAAAT 
GTACACAGTG CCAACGTTTC AAAAGTTTCT TTTAATGAGA AAGCTGTAAC TGAAACTTCC 
TTTAATTCCG TAAATGTTAA TGGCCAAAGA GGAGAGAATA GTAAACTTAG TCTTACCCCC 
AACTGTTCTT CAACTTTGAA CATTACACAA AGCCAAATAC ATTTTCTAAG TCCAGATTCT 
TTTGTAAATA ATAGTCATGG AGCTAATAAT GAACTAGAAT TAGTAACATG TCTTTCATCA 
GATATGTTTA TGAAAGATAA TTCACAGCCT GTGCATTTGG AATCAACAAT TGCACATGAA 
ATTTATCAGA AAATTTTAAG TCCAGATTCT TTCATAAAAG ATAATTATGG ACTAAATCAG 
GATCTAGAAT CAGAGTCAGT TAATCCTATT TTATCCCCTA ATCAATTTTT AAAAGATAAC 
ATGGCATATA TGTGTACATC TCAGCAAACA TGTAAAGTAC CATTATCAAA TGAAAATTCT 
CAAGTCCCAC AGTCTCCTGA AGATTGGAGA AAAAGTGAAG TTTCGCCACG TATTCCTGAA 
TGTCAGGGTT CAAAATCTCC CAAAGCTATT TTTGAAGAAC TAGTAGAAAT GAAGTCAAAT 
TACTACAGTT TTATAAAACA AAATAATCCT AAATTTTCTG CAGTTCAGGA TATTTCTAGT 
CATAGCCACA ATAAACAACC TAAGAGACGT CCAATACTTT CTGCCACTGT TACTAAAAGG 
AAGGCCACCT GTACCAGAGA AAACCAAACT GAGATTAATA AACCAAAAGC AAAAAGATGT 
CTCAACAGTG CAGTGGGTGA ACATGAAAAA GTAATAAATA ATCAAAAGGA AAAAGAAGAT 
TTTCATTCTT ATCTTCCAAT TATAGATCCA ATATTAAGTA AATCTAAGAG TTATAAAAAC 
GAGGTAACAC CCTCTTCGAC AACAGCTTCA GTTGCTCGGA AAAGAAAGAG CGATGGAAGC 
ATGGAAGATG CAAATGTGAG AGTTGCAATT ACAGAACATA CAGAAGTGCG AGAAATCAAA 
AGAATCCATT TTTCTCCCTC AGAGCCTAAA ACATCAGCTG TTAAGAAAAC AAAAAATGTG 
ACAACACCCA TCTCAAAACG TATTAGCAAC AGAGAGAAAT TAAACCTGAA GAAGAAAACT 
GATTTATCAA TATTCAGAAC TCCAATTTCT AAAACAAACA AAAGGACAAA ACCCATTATC 
GCTGTGGCAC AGTCCAGTTT GACCTTCATA AAACCATTAA AAACAGATAT TCCCAGACAC 
CCGATGCCAT TTGCTGCAAA AAACATGTTT TATGATGAAC GCTGGAAGGA AAAGCAGGAA 
CAGGGCTTCA CTTGGTGGTT AAATTTTATA TTAACCCCTG ATGACTTCAC TGTAAAAACA 
AATATTTCTG AAGTAAATGC TGCTACTCTT CTTTTGGGAA TAGAGAATCA ACATAAAATA 
AGTGTTCCTA GAGCACCTAC AAAAGAGGAA ATGTCTCTCA GAGCTTATAC TGCTCGGTGT 
AGGTTAAACA GACTACGTCG TGCAGCATGC CGTTTGTTTA CTTCTGAAAA AATGGTTAAA 
GCTATTAAAA AGCTTGAAAT TGAAATTGAA GCTAGGCGGT TAATTGTTCG AAAAGATAGA 
CACCTATGGA AAGATGTGGG AGAACGTCAG AAAGTCCTGA ATTGGCTGTT GTCCTACAAT 
CCTTTGTGGC TTCGAATTGG TCTAGAGACA ACTTATGGAG AACTCATATC TTTGGAAGAT 
AACAGTGATG TCACAGGGTT GGCTATGTTT ATTCTGAATC GCCTACTTTG GAATCCTGAT 
ATAGCAGCTG AGTATAGACA CCCCACTGTT CCTCACCTGT ATAGAGATGG TCATGAAGAA 
GCTTTGTCCA AGTTTACATT GAAAAAGTTA TTGTTGTTGG TCTGTTTTCT TGATTATGCT 
AAAATTTCCA GACTCATTGA TCATGATCCT TGTCTCTTCT GTAAAGATGC CGAATTCAAG 
GCTAGTAAAG AAATCCTTTT GGCTTTTTCA CGAGATTTCC TAAGTGGTGA AGGTGACCTT 
TCCCGTCACC TTGGCTTATT GGGATTACCT GTTAACCATG TTCAGACACC ATTTGATGAA 
TTTGATTTTG CCGTTACAAA TCTTGCCGTA GACTTGCAAT GTGGAGTGCG CCTTGTGCGA 
ACCATGGAAC TTCTCACACA GAACTGGGAC CTCTCAAAGA AACTCAGGAT TCCGGCAATA 
AGTCGTCTTC AAAAGATGCA CAATGTTGAC ATTGTTCTTC AAGTTCTTAA ATCACGAGGA 
ATTGAATTAA GTGATGAGCA TGGAAATACA ATTCTATCTA AGGATATTGT GGATAGGCAC 
AGAGAAAAAA CTCTCAGGTT GCTTTGGAAA ATAGCGTTTG CTTTTCAGGT GGATATTTCC 
CTTAACTTAG ATCAATTAAA GGAAGAAATT GCCTTTCTAA AACACACAAA GAGTATAAAG 
AAAACAATAT CTCTACTATC ATGCCATTCT GATGATCTTA TTAATAAGAA AAAAGGCAAA 
AGGGATAGTG GTTCCTTTGA ACAATATAGT GAAAACATAA AGTTATTGAT GGATTGGGTA 
AATGCTGTTT GTGCCTTCTA TAATAAAAAG GTGGAGAATT TTACAGTGTC TTTCTCAGAC 
GGCCGTGTGT TATGTTACCT GATCCACCAT TACCATCCTT GCTATGTGCC ATTTGACGCT 
ATATGTCAGC GTACTACTCA AACTGTGGAA TGTACGCAAA CTGGTTCAGT GGTATTAAAT 
TCATCATCTG AATCTGATGA CAGTTCTCTG GATATGTCTC TTAAAGCATT TGATCATGAA 
AATACTTCAG AGCTATACAA AGAGCTCCTA GAAAATGAAA AGAAAAATTT TCACTTGGTT 
AGGTCTGCAG TTAGAGACCT TGGTGGAATA CCTGCTATGA TTAATCATTC AGATATGTCA 
AATACAATTC CAGATGAAAA GGTGGTTATT ACCTATTTGT CATTTCTTTG TGCAAGGCTT 
TTGGATCTTC GTAAAGAAAT AAGAGCTGCT CGACTCATAC AAACAACATG GAGAAAATAT 
AAACTAAAAA CAGATCTCAA ACGCCATCAG GAGAGAGAGA AAGCTGCAAG AATTATTCAA 
TTGGCTGTAA TCAATTTTCT AGCAAAACAA AGATTGAGAA AAAGAGTTAA TGCAGCACTC 
GTCATTCAGA AATATTGGCG AAGAGTCTTA GCACAGAGAA AATTATTAAT GTTAAAAAAG 
GAAAAGCTGG AAAAAGTTCA AAATAAAGCA GCATCACTTA TTCAGGGATA TTGGAGAAGA 
TATTCCACTA GACAAAGATT TCTGAAATTG AAATATTATT CAATCATCCT GCAATCTAGG 
ATAAGAATGA TAATTGCTGT TACATCTTAT AAACGATATC TTTGGGCTAC AGTTACAATT 
CAGAGGCATT GGCGTGCTTA TTTAAGAAGA AAACAAGATC AACAAAGATA TGAAATGCTA 
AAATCATCAA CTCTTATAAT CCAATCTATG TTCAGAAAAT GGAAGCAACG TAAAATGCAA 
TCACAAGTAA AAGCTACAGT AATATTGCAA AGAGCTTTTA GAGAATGGCA TTTAAGAAAA 
CAAGCTAAAG AAGAAAATTC TGCTATTATC ATACAATCAT GGTATAGAAT GCATAAAGAA 
TTACGGAAAT ATATTTATAT TAGATCTTGT GTTGTTATCA TTCAGAAAAG ATTTCGGTGC 
TTTCAAGCCC AAAAGTTATA TAAAAGAAGA AAAGAGTCCA TACTAACCAT CCAGAAGTAC 
TACAAAGCAT ATCTGAAAGG AAAGATTGAG CGCACCAACT ATTTGCAGAA ACGAGCTGCA 
GCCATTCAAT TACAAGCTGC TTTTAGGAGA CTGAAAGCTC ATAATTTATG TAGACAAATT 
AGAGCTGCTT GTGTTATTCA GTCATACTGG AGAATGAGAC AAGACAGAGT TCGATTTTTA 
AACCTTAAGA AGACTATTAT CAAATTTCAG GCACATGTAA GAAAACATCA ACAACGACAG 
AAATATAAGA AGATGAAGAA AGCAGCTGTT ATAATTCAGA CTCATTTCCG AGCTTATATT 
TTTGCCATGA AAGTTCTAGC ATCTTACCAG AAAACACGCT CTGCTGTCAT TGTGCTGCAG 
TCTGCATATA GAGGGATGCA AGCCAGGAAA ATGTATATTC ACATCCTCAC ATCTGTTATA 
AAGATTCAAT CATATTATCG TGCTTATGTT TCTAAAAAGG AATTTTTGAG CCTAAAAAAT 
GCTACAATAA AATTGCAGTC AACTGTTAAG ATGAAACAAA CACGTAAACA ATATTTGCAT 
TTAAGAGCAG CTGCACTATT TATCCAGCAA TGTTACCGTT CCAAAAAAAT AGCTGCACAA 
AAGAGAGAAG AGTATATGCA GATGCGGGAA TCTTGTATCA AACTGCAAGC ATTTGTTAGA 
GGATACCTTG TCCGAAAGCA GATGAGGTTA CAAAGAAAAG CTGTTATTTC ACTACAGTCT 
TATTTCAGAA TGAGAAAGGC TCGGCAGTAT TATCTGAAAA TGTATAAAGC AATTATTGTC 
ATTCAGAATT ACTATCATGC ATACAAAGCA CAGGTCAATC AGAGGAAGAA CTTCTTGCAA 
GTCAAAAAAG CAGCTACTTG CTTGCAAGCA GCTTACAGAG GTTATAAAGT ACGCCAGCTA 
ATCAAACAAC AATCTATAGC TGCTCTTAAA ATTCAGTCTG CTTTTAGAGG CTATAATAAA 
AGGGTAAAAT ATCAATCTGT GCTTCAATCT ATAATAAAGA TTCAGAGATG GTACAGGGCG 
TACAAGACTC TTCATGATAC AAGAACACAT TTTTTGAAGA CAAAGGCAGC TGTGATTTCC 
CTCCAGTCTG CTTATCGTGG CTGGAAGGTT CGGAAACAGA TTAGAAGGGA ACATCAAGCT 
GCCTTGAAGA TTCAGTCTGC TTTTAGAATG GCCAAGGCCC AGAAACAGTT TAGATTGTTT 
AAAACAGCAG CATTAGTCAT CCAGCAAAAT TTCAGAGCAT GGACTGCAGG AAGGAAGCAA 
TGTATGGAGT ATATTGAACT CCGTCATGCG GTACTGGTGC TTCAATCTAT GTGGAAGGGA 
AAAACACTGA GAAGACAGCT TCAAAGGCAA CATAAATGTG CTATCATCAT ACAGTCATAC 
TATAGAATGC ATGTGCAACA AAAGAAGTGG AAAATCATGA AAAAAGCTGC TCTTCTGATT 
CAAAAGTATT ATAGGGCTTA CAGTATTGGA AGAGAACAGA ATCATTTATA TTTGAAAACA 
AAAGCAGCTG TAGTAACTTT ACAGTCAGCT TATCGTGGTA TGAAAGTGAG AAAAAGAATA 
AAGGATTGCA ACAAAGCAGC AGTCACTATA CAGTCTAAAT ACAGAGCTTA CAAAACCAAA 
AAGAAATATG CAACCTATAG AGCTTCAGCT ATTATAATTC AGAGATGGTA TCGAGGTATT 
AAAATTACAA ACCATCAGCA TAAGGAGTAT CTTAATTTGA AGAAGACAGC AATTAAAATC 
CAATCTGTTT ATAGAGGTAT TAGAGTTAGA AGACATATTC AACACATGCA CAGGGCAGCC 
ACTTTTATTA AAGCCATGTT TAAAATGCAT CAGTCAAGAA TAAGTTACCA TACAATGAGA 
AAAGCAGCTA TTGTTATTCA AGTAAGATGT AGAGCATATT ATCAAGGTAA AATGCAGCGT 
GAAAAGTACC TGACAATTTT GAAAGCTGTT AAAGTCCTTC AGGCAAGTTT TAGAGGAGTA 
AGAGTTAGAC GGACTCTTAG AAAGATGCAG ACTGCAGCAA CACTCATTCA GTCAAACTAC 
AGAAGATACA GACAGCAAAC ATACTTTAAT AAGTTAAAGA AAATAACAAA AACAGTACAG 
CAAAGATACT GGGCAATGAA AGAAAGAAAC ATACAATTTC AAAGGTATAA CAAACTGAGG 
CATTCTGTAA TATACATTCA GGCTATTTTT AGGGGAAAGA AAGCTAGAAG ACATTTAAAA 
ATGATGCATA TAGCCGCAAC TCTCATTCAG AGGAGATTTA GAACTCTAAT GATGAGAAGA 
AGATTCCTCT CTCTCAAGAA AACTGCTATT TTGATTCAGA GAAAATATCG GGCACATCTT 
TGTACAAAGC ATCACTTACA GTTCCTTCAG GTACAAAATG CAGTTATTAA AATCCAGTCA 
TCATACAGAA GATGGATGAT AAGGAAAAGG ATGCGAGAGA TGCACAGGGC TGCTACTTTC 
ATCCAGTCTA CTTTCAGAAT GCACAGATTA CATATGAGAT ATCAGGCTTT GAAACAGGCC 
TCCGTTGTGA TCCAACAGCA ATACCAAGCA AATAGAGCTG CAAAACTGCA GAGGCAGCAT 
TATCTCAGAC AAAGACACTC TGCTGTGATC CTTCAGGCTG CATTCAGGGG TATGAAAACT 
AGAAGACATT TGAAGAGTAT GCATTCCTCT GCAACCCTTA TTCAGAGTAG GTTTAGATCA 
TTACTGGTGA GGAGAAGATT CATTTCCCTC AAAAAAGCTA CTATTTTTGT TCAGAGGAAA 
TATCGAGCCA CCATTTGTGC CAAACATAAA TTGTACCAAT TCTTGCACTT AAGAAAGGCA 
GCCATTACAA TACAGTCATC TTACAGAAGA CTGATGGTAA AGAAGAAGTT ACAAGAAATG 
CAAAGGGCTG CAGTTCTCAT TCAGGCTACT TTCAGGATGT ACAGAACATA TATTACATTT 
CAGACTTGGA AACATGCTTC AATTCTAATT CAGCAACATT ATCGAACATA TAGAGCTGCA 
AAATTACAAA GAGAAAATTA TATCAGACAA TGGCATTCTG CTGTGGTTAT TCAGGCTGCA 
TATAAAGGAA TGAAAGCAAG ACAACTTTTA AGGGAAAAAC ACAAAGCTTC TATCGTAATA 
CAAAGCACCT ACAGAATGTA TAGGCAGTAT TGTTTCTACC AAAAGCTTCA GTGGGCTACA 
AAAATCATAC AAGAAAAATA TAGAGCAAAT AAAAAGAAAC AGAAAGTATT TCAACACAAT 
GAACTTAAGA AAGAGACTTG TGTTCAGGCA GGTTTTCAGG ACATGAACAT AAAAAAACAG 
ATTCAGGAAC AGCACCAGGC TGCCATTATT ATTCAGAAGC ATTGTAAAGC CTTTAAAATA 
AGGAAGCATT ATCTCCACCT TAGAGCAACA GTAGTTTCTA TTCAAAGAAG ATACAGAAAA 
CTAACTGCAG TGCGTACCCA AGCAGTTATT TGTATACAGT CTTATTACAG AGGCTTTAAA 
GTACGAAAGG ATATTCAAAA TATGCACCGG GCTGCCACAC TAATTCAGTC ATTCTATCGA 
ATGCACAGGG CCAAAGTTGA TTATGAAACA AAGAAAACTG CAATTGTGGT TATACAGAAT 
TATTATAGGT TGTATGTTAG AGTAAAAACA GAAAGAAAAA ACTTTTTAGC AGTTCAGAAA 
TCTGTACGAA CTATTCAGGC TGCTTTTAGA GGCATGAAAG TTAGACAAAA ATTGAAAAAT 
GTATCAGAGG AAAAGATGGC AGCCATTGTT AACCAATCTG CACTCTGCTG TTACAGAAGT 
AAAACTCAGT ATGAAGCTGT TCAAAGTGAA GGTGTTATGA TTCAAGAGTG GTATAAAGCT 
TCTGGCCTTG CTTGTTCACA GGAAGCAGAG TATCATTCTC AAAGTAGGGC TGCAGTAACA 
ATTCAAAAAG CTTTTTGTAG AATGGTCACA AGAAAACTGG AAACACAGAA ATGTGCTGCC 
CTACGGATTC AGTTCTTCCT TCAGATGGCT GTGTATCGGA GAAGATTTGT TCAGCAGAAA 
AGAGCTGCTA TCACTTTACA GCATTATTTT AGGACGTGGC AAACCAGAAA ACAGTTTTTA 
CTATATAGAA AAGCAGCAGT GGTTTTACAA AATCACTACA GAGCATTTCT GTCTGCAAAA 
CATCAAAGAC AAGTCTATTT ACAGATCAGA AGCAGTGTTA TCATTATTCA AGCTAGAAGT 
AAAGGATTTA TACAGAAACG GAAGTTTCAG GAAATTAAAA ATAGCACCAT AAAAATTCAG 
GCTATGTGGA GGAGATATAG AGCCAAGAAA TATTTATGTA AAGTGAAAGC TGCCTGCAAG 
ATTCAAGCCT GGTATAGATG TTGGAGAGCA CACAAAGAAT ATCTAGCTAT ATTAAAAGCT 
GTTAAAATTA TTCAAGGTTG CTTCTATACC AAACTAGAGA GAACACGGTT TTTGAATGTG 
AGAGCATCAG CAATTATCAT TCAGAGAAAA TGGAGAGCTA TACTTCCTGC AAAGATAGCT 
CATGAACACT TCTTAATGAT AAAAAGACAT CGAGCTGCTT GTTTGATCCA AGCACATTAT 
AGAGGATATA AAGGAAGGCA GGTCTTTCTT CGGCAGAAAT CTGCTGCTTT GATCATACAA 
AAATATATAC GAGCCAGGGA GGCTGGAAAG CATGAAAGGA TAAAATATAT TGAATTTAAA 
AAATCTACAG TTATCCTACA AGCACTGGTG CGTGGTTGGC TAGTACGAAA AAGATTTTTA 
GAACAGAGAG CCAAAATTCG ACTTCTTCAC TTCACTGCAG CTGCATATTA TCACCTGAAT 
GCTGTTAGAA TTCAAAGAGC CTATAAACTT TACCTGGCTG TGAAGAATGC TAACAAGCAG 
GTTAATTCAG TCATCTGTAT TCAGAGATGG TTTCGAGCAA GATTACAAGA AAAGAGATTT 
ATTCAGAAAT ATCATAGCAT CAAAAAGATT GAGCATGAAG GTCAAGAATG TCTGAGCCAG 
CGAAATAGGG CTGCATCAGT AATACAGAAA GCAGTGCGCC ATTTTCTCCT CCGTAAAAAG 
CAGGAAAAAT TCACTAGTGG AATCATTAAA ATTCAGGCAT TATGGAGAGG CTATTCTTGG 
AGGAAGAAAA ATGATTGTAC AAAAATTAAA GCTATACGAC TAAGTCTTCA AGTTGTTAAT 
AGGGAGATTC GAGAAGAAAA CAAACTCTAC AAAAGAACTG CACTTGCACT TCATTACCTT 
TTGACATATA AGCACCTTTC TGCCATTCTT GAGGCCTTAA AACACCTAGA GGTAGTTACT 
AGATTGTCTC CACTTTGTTG TGAGAACATG GCCCAGAGTG GAGCAATTTC TAAAATATTT 
GTTTTGATCC GAAGTTGTAA TCGCAGTATT CCTTGTATGG AAGTCATCAG ATATGCTGTG 
CAAGTCTTGC TTAATGTATC TAAGTATGAG AAAACTACTT CAGCAGTTTA TGATGTAGAA 
AATTGTATAG ATATACTATT GGAGCTTTTG CAGATATACC GAGAAAAGCC TGGTAATAAA 
GTTGCAGACA AAGGCGGAAG CATTTTTACA AAAACTTGTT GTTTGTTGGC TATTTTACTG 
AAGACAACAA ATAGAGCCTC TGATGTACGA AGTAGGTCCA AAGTTGTTGA CCGTATTTAC 
AGTCTCTACA AACTTACAGC TCATAAACAT AAAATGAATA CTGAAAGAAT ACTTTACAAG 
CAAAAGAAGA ATTCTTCTAT AAGCATTCCT TTTATCCCAG AAACACCTGT AAGGACCAGA 
ATAGTTTCAA GACTTAAGCC AGATTGGGTT TTGAGAAGAG ATAACATGGA AGAAATCACA 
AATCCCCTGC AAGCTATTCA AATGGTGATG GATACGCTTG GCATTCCTTA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060606.3
CDS	233..10666
Translation	MANRRVGRGCWEVSPTERRPPAGLRGPAAEEEASSPPVLSLSHFCRSPFLCFGDVLLGASRTLSLALDNPNEEVAEVKISHFPAADLGFSVSQRCFVLQPKEKIVISVNWTPLKEGRVREIMTFLVNDVLKHQAILLGNAEEQKKKKRSLWDTIKKKKISASTSHNRRVSNIQNVNKTFSVSQKVDRVRSPLQACENLAMNEGGPPTENNSLILEENKIPISPISPAFNECHGATCLPLSVRRSTTYSSLHASENRELLNVHSANVSKVSFNEKAVTETSFNSVNVNGQRGENSKLSLTPNCSSTLNITQSQIHFLSPDSFVNNSHGANNELELVTCLSSDMFMKDNSQPVHLESTIAHEIYQKILSPDSFIKDNYGLNQDLESESVNPILSPNQFLKDNMAYMCTSQQTCKVPLSNENSQVPQSPEDWRKSEVSPRIPECQGSKSPKAIFEELVEMKSNYYSFIKQNNPKFSAVQDISSHSHNKQPKRRPILSATVTKRKATCTRENQTEINKPKAKRCLNSAVGEHEKVINNQKEKEDFHSYLPIIDPILSKSKSYKNEVTPSSTTASVARKRKSDGSMEDANVRVAITEHTEVREIKRIHFSPSEPKTSAVKKTKNVTTPISKRISNREKLNLKKKTDLSIFRTPISKTNKRTKPIIAVAQSSLTFIKPLKTDIPRHPMPFAAKNMFYDERWKEKQEQGFTWWLNFILTPDDFTVKTNISEVNAATLLLGIENQHKISVPRAPTKEEMSLRAYTARCRLNRLRRAACRLFTSEKMVKAIKKLEIEIEARRLIVRKDRHLWKDVGERQKVLNWLLSYNPLWLRIGLETTYGELISLEDNSDVTGLAMFILNRLLWNPDIAAEYRHPTVPHLYRDGHEEALSKFTLKKLLLLVCFLDYAKISRLIDHDPCLFCKDAEFKASKEILLAFSRDFLSGEGDLSRHLGLLGLPVNHVQTPFDEFDFAVTNLAVDLQCGVRLVRTMELLTQNWDLSKKLRIPAISRLQKMHNVDIVLQVLKSRGIELSDEHGNTILSKDIVDRHREKTLRLLWKIAFAFQVDISLNLDQLKEEIAFLKHTKSIKKTISLLSCHSDDLINKKKGKRDSGSFEQYSENIKLLMDWVNAVCAFYNKKVENFTVSFSDGRVLCYLIHHYHPCYVPFDAICQRTTQTVECTQTGSVVLNSSSESDDSSLDMSLKAFDHENTSELYKELLENEKKNFHLVRSAVRDLGGIPAMINHSDMSNTIPDEKVVITYLSFLCARLLDLRKEIRAARLIQTTWRKYKLKTDLKRHQEREKAARIIQLAVINFLAKQRLRKRVNAALVIQKYWRRVLAQRKLLMLKKEKLEKVQNKAASLIQGYWRRYSTRQRFLKLKYYSIILQSRIRMIIAVTSYKRYLWATVTIQRHWRAYLRRKQDQQRYEMLKSSTLIIQSMFRKWKQRKMQSQVKATVILQRAFREWHLRKQAKEENSAIIIQSWYRMHKELRKYIYIRSCVVIIQKRFRCFQAQKLYKRRKESILTIQKYYKAYLKGKIERTNYLQKRAAAIQLQAAFRRLKAHNLCRQIRAACVIQSYWRMRQDRVRFLNLKKTIIKFQAHVRKHQQRQKYKKMKKAAVIIQTHFRAYIFAMKVLASYQKTRSAVIVLQSAYRGMQARKMYIHILTSVIKIQSYYRAYVSKKEFLSLKNATIKLQSTVKMKQTRKQYLHLRAAALFIQQCYRSKKIAAQKREEYMQMRESCIKLQAFVRGYLVRKQMRLQRKAVISLQSYFRMRKARQYYLKMYKAIIVIQNYYHAYKAQVNQRKNFLQVKKAATCLQAAYRGYKVRQLIKQQSIAALKIQSAFRGYNKRVKYQSVLQSIIKIQRWYRAYKTLHDTRTHFLKTKAAVISLQSAYRGWKVRKQIRREHQAALKIQSAFRMAKAQKQFRLFKTAALVIQQNFRAWTAGRKQCMEYIELRHAVLVLQSMWKGKTLRRQLQRQHKCAIIIQSYYRMHVQQKKWKIMKKAALLIQKYYRAYSIGREQNHLYLKTKAAVVTLQSAYRGMKVRKRIKDCNKAAVTIQSKYRAYKTKKKYATYRASAIIIQRWYRGIKITNHQHKEYLNLKKTAIKIQSVYRGIRVRRHIQHMHRAATFIKAMFKMHQSRISYHTMRKAAIVIQVRCRAYYQGKMQREKYLTILKAVKVLQASFRGVRVRRTLRKMQTAATLIQSNYRRYRQQTYFNKLKKITKTVQQRYWAMKERNIQFQRYNKLRHSVIYIQAIFRGKKARRHLKMMHIAATLIQRRFRTLMMRRRFLSLKKTAILIQRKYRAHLCTKHHLQFLQVQNAVIKIQSSYRRWMIRKRMREMHRAATFIQSTFRMHRLHMRYQALKQASVVIQQQYQANRAAKLQRQHYLRQRHSAVILQAAFRGMKTRRHLKSMHSSATLIQSRFRSLLVRRRFISLKKATIFVQRKYRATICAKHKLYQFLHLRKAAITIQSSYRRLMVKKKLQEMQRAAVLIQATFRMYRTYITFQTWKHASILIQQHYRTYRAAKLQRENYIRQWHSAVVIQAAYKGMKARQLLREKHKASIVIQSTYRMYRQYCFYQKLQWATKIIQEKYRANKKKQKVFQHNELKKETCVQAGFQDMNIKKQIQEQHQAAIIIQKHCKAFKIRKHYLHLRATVVSIQRRYRKLTAVRTQAVICIQSYYRGFKVRKDIQNMHRAATLIQSFYRMHRAKVDYETKKTAIVVIQNYYRLYVRVKTERKNFLAVQKSVRTIQAAFRGMKVRQKLKNVSEEKMAAIVNQSALCCYRSKTQYEAVQSEGVMIQEWYKASGLACSQEAEYHSQSRAAVTIQKAFCRMVTRKLETQKCAALRIQFFLQMAVYRRRFVQQKRAAITLQHYFRTWQTRKQFLLYRKAAVVLQNHYRAFLSAKHQRQVYLQIRSSVIIIQARSKGFIQKRKFQEIKNSTIKIQAMWRRYRAKKYLCKVKAACKIQAWYRCWRAHKEYLAILKAVKIIQGCFYTKLERTRFLNVRASAIIIQRKWRAILPAKIAHEHFLMIKRHRAACLIQAHYRGYKGRQVFLRQKSAALIIQKYIRAREAGKHERIKYIEFKKSTVILQALVRGWLVRKRFLEQRAKIRLLHFTAAAYYHLNAVRIQRAYKLYLAVKNANKQVNSVICIQRWFRARLQEKRFIQKYHSIKKIEHEGQECLSQRNRAASVIQKAVRHFLLRKKQEKFTSGIIKIQALWRGYSWRKKNDCTKIKAIRLSLQVVNREIREENKLYKRTALALHYLLTYKHLSAILEALKHLEVVTRLSPLCCENMAQSGAISKIFVLIRSCNRSIPCMEVIRYAVQVLLNVSKYEKTTSAVYDVENCIDILLELLQIYREKPGNKVADKGGSIFTKTCCLLAILLKTTNRASDVRSRSKVVDRIYSLYKLTAHKHKMNTERILYKQKKNSSISIPFIPETPVRTRIVSRLKPDWVLRRDNMEEITNPLQAIQMVMDTLGIPY

Target ORF information:

RefSeq Version	NM_018136.5
Organism	Homo sapiens(human)
Definition	Homo sapiens assembly factor for spindle microtubules (ASPM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018136.5

ORF Insert Sequence:

ATGGCGAACC GGCGAGTGGG GCGAGGCTGC TGGGAAGTGA GCCCGACCGA GCGGAGGCCG 
CCCGCGGGGC TGCGGGGCCC CGCGGCCGAG GAGGAGGCGT CTTCCCCGCC GGTCCTGTCT 
CTCAGCCACT TCTGCAGGTC TCCTTTCCTT TGCTTCGGGG ACGTTCTCCT GGGAGCCTCA 
CGGACGCTGT CTCTGGCCCT AGACAACCCT AACGAGGAGG TGGCAGAAGT GAAGATCTCC 
CACTTCCCGG CCGCGGACCT GGGCTTCAGT GTGTCGCAGC GCTGTTTCGT GTTGCAGCCT 
AAAGAGAAAA TTGTTATTTC TGTTAACTGG ACACCACTCA AAGAAGGCCG AGTAAGAGAG 
ATTATGACAT TTCTTGTAAA TGATGTTCTG AAACACCAAG CTATATTACT AGGAAATGCA 
GAAGAGCAGA AAAAGAAAAA GAGGAGTCTT TGGGATACCA TTAAAAAGAA GAAAATTTCA 
GCCTCTACAA GTCACAACAG AAGGGTTTCA AATATTCAGA ATGTTAATAA AACATTTAGT 
GTTTCCCAAA AAGTTGACAG AGTTAGGAGC CCACTACAAG CTTGTGAAAA CTTGGCTATG 
AATGAAGGCG GTCCCCCAAC AGAAAACAAT TCTTTAATAC TTGAAGAAAA TAAAATACCC 
ATATCACCTA TTAGCCCTGC TTTCAATGAA TGCCATGGTG CAACTTGCTT GCCACTCTCT 
GTACGTCGAT CTACTACCTA CTCATCTCTT CATGCATCAG AAAATAGGGA ACTATTAAAT 
GTACACAGTG CCAACGTTTC AAAAGTTTCT TTTAATGAGA AAGCTGTAAC TGAAACTTCC 
TTTAATTCCG TAAATGTTAA TGGCCAAAGA GGAGAGAATA GTAAACTTAG TCTTACCCCC 
AACTGTTCTT CAACTTTGAA CATTACACAA AGCCAAATAC ATTTTCTAAG TCCAGATTCT 
TTTGTAAATA ATAGTCATGG AGCTAATAAT GAACTAGAAT TAGTAACATG TCTTTCATCA 
GATATGTTTA TGAAAGATAA TTCACAGCCT GTGCATTTGG AATCAACAAT TGCACATGAA 
ATTTATCAGA AAATTTTAAG TCCAGATTCT TTCATAAAAG ATAATTATGG ACTAAATCAG 
GATCTAGAAT CAGAGTCAGT TAATCCTATT TTATCCCCTA ATCAATTTTT AAAAGATAAC 
ATGGCATATA TGTGTACATC TCAGCAAACA TGTAAAGTAC CATTATCAAA TGAAAATTCT 
CAAGTCCCAC AGTCTCCTGA AGATTGGAGA AAAAGTGAAG TTTCGCCACG TATTCCTGAA 
TGTCAGGGTT CAAAATCTCC CAAAGCTATT TTTGAAGAAC TAGTAGAAAT GAAGTCAAAT 
TACTACAGTT TTATAAAACA AAATAATCCT AAATTTTCTG CAGTTCAGGA TATTTCTAGT 
CATAGCCACA ATAAACAACC TAAGAGACGT CCAATACTTT CTGCCACTGT TACTAAAAGG 
AAGGCCACCT GTACCAGAGA AAACCAAACT GAGATTAATA AACCAAAAGC AAAAAGATGT 
CTCAACAGTG CAGTGGGTGA ACATGAAAAA GTAATAAATA ATCAAAAGGA AAAAGAAGAT 
TTTCATTCTT ATCTTCCAAT TATAGATCCA ATATTAAGTA AATCTAAGAG TTATAAAAAC 
GAGGTAACAC CCTCTTCGAC AACAGCTTCA GTTGCTCGGA AAAGAAAGAG CGATGGAAGC 
ATGGAAGATG CAAATGTGAG AGTTGCAATT ACAGAACATA CAGAAGTGCG AGAAATCAAA 
AGAATCCATT TTTCTCCCTC AGAGCCTAAA ACATCAGCTG TTAAGAAAAC AAAAAATGTG 
ACAACACCCA TCTCAAAACG TATTAGCAAC AGAGAGAAAT TAAACCTGAA GAAGAAAACT 
GATTTATCAA TATTCAGAAC TCCAATTTCT AAAACAAACA AAAGGACAAA ACCCATTATC 
GCTGTGGCAC AGTCCAGTTT GACCTTCATA AAACCATTAA AAACAGATAT TCCCAGACAC 
CCGATGCCAT TTGCTGCAAA AAACATGTTT TATGATGAAC GCTGGAAGGA AAAGCAGGAA 
CAGGGCTTCA CTTGGTGGTT AAATTTTATA TTAACCCCTG ATGACTTCAC TGTAAAAACA 
AATATTTCTG AAGTAAATGC TGCTACTCTT CTTTTGGGAA TAGAGAATCA ACATAAAATA 
AGTGTTCCTA GAGCACCTAC AAAAGAGGAA ATGTCTCTCA GAGCTTATAC TGCTCGGTGT 
AGGTTAAACA GACTACGTCG TGCAGCATGC CGTTTGTTTA CTTCTGAAAA AATGGTTAAA 
GCTATTAAAA AGCTTGAAAT TGAAATTGAA GCTAGGCGGT TAATTGTTCG AAAAGATAGA 
CACCTATGGA AAGATGTGGG AGAACGTCAG AAAGTCCTGA ATTGGCTGTT GTCCTACAAT 
CCTTTGTGGC TTCGAATTGG TCTAGAGACA ACTTATGGAG AACTCATATC TTTGGAAGAT 
AACAGTGATG TCACAGGGTT GGCTATGTTT ATTCTGAATC GCCTACTTTG GAATCCTGAT 
ATAGCAGCTG AGTATAGACA CCCCACTGTT CCTCACCTGT ATAGAGATGG TCATGAAGAA 
GCTTTGTCCA AGTTTACATT GAAAAAGTTA TTGTTGTTGG TCTGTTTTCT TGATTATGCT 
AAAATTTCCA GACTCATTGA TCATGATCCT TGTCTCTTCT GTAAAGATGC CGAATTCAAG 
GCTAGTAAAG AAATCCTTTT GGCTTTTTCA CGAGATTTCC TAAGTGGTGA AGGTGACCTT 
TCCCGTCACC TTGGCTTATT GGGATTACCT GTTAACCATG TTCAGACACC ATTTGATGAA 
TTTGATTTTG CCGTTACAAA TCTTGCCGTA GACTTGCAAT GTGGAGTGCG CCTTGTGCGA 
ACCATGGAAC TTCTCACACA GAACTGGGAC CTCTCAAAGA AACTCAGGAT TCCGGCAATA 
AGTCGTCTTC AAAAGATGCA CAATGTTGAC ATTGTTCTTC AAGTTCTTAA ATCACGAGGA 
ATTGAATTAA GTGATGAGCA TGGAAATACA ATTCTATCTA AGGATATTGT GGATAGGCAC 
AGAGAAAAAA CTCTCAGGTT GCTTTGGAAA ATAGCGTTTG CTTTTCAGGT GGATATTTCC 
CTTAACTTAG ATCAATTAAA GGAAGAAATT GCCTTTCTAA AACACACAAA GAGTATAAAG 
AAAACAATAT CTCTACTATC ATGCCATTCT GATGATCTTA TTAATAAGAA AAAAGGCAAA 
AGGGATAGTG GTTCCTTTGA ACAATATAGT GAAAACATAA AGTTATTGAT GGATTGGGTA 
AATGCTGTTT GTGCCTTCTA TAATAAAAAG GTGGAGAATT TTACAGTGTC TTTCTCAGAC 
GGCCGTGTGT TATGTTACCT GATCCACCAT TACCATCCTT GCTATGTGCC ATTTGACGCT 
ATATGTCAGC GTACTACTCA AACTGTGGAA TGTACGCAAA CTGGTTCAGT GGTATTAAAT 
TCATCATCTG AATCTGATGA CAGTTCTCTG GATATGTCTC TTAAAGCATT TGATCATGAA 
AATACTTCAG AGCTATACAA AGAGCTCCTA GAAAATGAAA AGAAAAATTT TCACTTGGTT 
AGGTCTGCAG TTAGAGACCT TGGTGGAATA CCTGCTATGA TTAATCATTC AGATATGTCA 
AATACAATTC CAGATGAAAA GGTGGTTATT ACCTATTTGT CATTTCTTTG TGCAAGGCTT 
TTGGATCTTC GTAAAGAAAT AAGAGCTGCT CGACTCATAC AAACAACATG GAGAAAATAT 
AAACTAAAAA CAGATCTCAA ACGCCATCAG GAGAGAGAGA AAGCTGCAAG AATTATTCAA 
TTGGCTGTAA TCAATTTTCT AGCAAAACAA AGATTGAGAA AAAGAGTTAA TGCAGCACTC 
GTCATTCAGA AATATTGGCG AAGAGTCTTA GCACAGAGAA AATTATTAAT GTTAAAAAAG 
GAAAAGCTGG AAAAAGTTCA AAATAAAGCA GCATCACTTA TTCAGGGATA TTGGAGAAGA 
TATTCCACTA GACAAAGATT TCTGAAATTG AAATATTATT CAATCATCCT GCAATCTAGG 
ATAAGAATGA TAATTGCTGT TACATCTTAT AAACGATATC TTTGGGCTAC AGTTACAATT 
CAGAGGCATT GGCGTGCTTA TTTAAGAAGA AAACAAGATC AACAAAGATA TGAAATGCTA 
AAATCATCAA CTCTTATAAT CCAATCTATG TTCAGAAAAT GGAAGCAACG TAAAATGCAA 
TCACAAGTAA AAGCTACAGT AATATTGCAA AGAGCTTTTA GAGAATGGCA TTTAAGAAAA 
CAAGCTAAAG AAGAAAATTC TGCTATTATC ATACAATCAT GGTATAGAAT GCATAAAGAA 
TTACGGAAAT ATATTTATAT TAGATCTTGT GTTGTTATCA TTCAGAAAAG ATTTCGGTGC 
TTTCAAGCCC AAAAGTTATA TAAAAGAAGA AAAGAGTCCA TACTAACCAT CCAGAAGTAC 
TACAAAGCAT ATCTGAAAGG AAAGATTGAG CGCACCAACT ATTTGCAGAA ACGAGCTGCA 
GCCATTCAAT TACAAGCTGC TTTTAGGAGA CTGAAAGCTC ATAATTTATG TAGACAAATT 
AGAGCTGCTT GTGTTATTCA GTCATACTGG AGAATGAGAC AAGACAGAGT TCGATTTTTA 
AACCTTAAGA AGACTATTAT CAAATTTCAG GCACATGTAA GAAAACATCA ACAACGACAG 
AAATATAAGA AGATGAAGAA AGCAGCTGTT ATAATTCAGA CTCATTTCCG AGCTTATATT 
TTTGCCATGA AAGTTCTAGC ATCTTACCAG AAAACACGCT CTGCTGTCAT TGTGCTGCAG 
TCTGCATATA GAGGGATGCA AGCCAGGAAA ATGTATATTC ACATCCTCAC ATCTGTTATA 
AAGATTCAAT CATATTATCG TGCTTATGTT TCTAAAAAGG AATTTTTGAG CCTAAAAAAT 
GCTACAATAA AATTGCAGTC AACTGTTAAG ATGAAACAAA CACGTAAACA ATATTTGCAT 
TTAAGAGCAG CTGCACTATT TATCCAGCAA TGTTACCGTT CCAAAAAAAT AGCTGCACAA 
AAGAGAGAAG AGTATATGCA GATGCGGGAA TCTTGTATCA AACTGCAAGC ATTTGTTAGA 
GGATACCTTG TCCGAAAGCA GATGAGGTTA CAAAGAAAAG CTGTTATTTC ACTACAGTCT 
TATTTCAGAA TGAGAAAGGC TCGGCAGTAT TATCTGAAAA TGTATAAAGC AATTATTGTC 
ATTCAGAATT ACTATCATGC ATACAAAGCA CAGGTCAATC AGAGGAAGAA CTTCTTGCAA 
GTCAAAAAAG CAGCTACTTG CTTGCAAGCA GCTTACAGAG GTTATAAAGT ACGCCAGCTA 
ATCAAACAAC AATCTATAGC TGCTCTTAAA ATTCAGTCTG CTTTTAGAGG CTATAATAAA 
AGGGTAAAAT ATCAATCTGT GCTTCAATCT ATAATAAAGA TTCAGAGATG GTACAGGGCG 
TACAAGACTC TTCATGATAC AAGAACACAT TTTTTGAAGA CAAAGGCAGC TGTGATTTCC 
CTCCAGTCTG CTTATCGTGG CTGGAAGGTT CGGAAACAGA TTAGAAGGGA ACATCAAGCT 
GCCTTGAAGA TTCAGTCTGC TTTTAGAATG GCCAAGGCCC AGAAACAGTT TAGATTGTTT 
AAAACAGCAG CATTAGTCAT CCAGCAAAAT TTCAGAGCAT GGACTGCAGG AAGGAAGCAA 
TGTATGGAGT ATATTGAACT CCGTCATGCG GTACTGGTGC TTCAATCTAT GTGGAAGGGA 
AAAACACTGA GAAGACAGCT TCAAAGGCAA CATAAATGTG CTATCATCAT ACAGTCATAC 
TATAGAATGC ATGTGCAACA AAAGAAGTGG AAAATCATGA AAAAAGCTGC TCTTCTGATT 
CAAAAGTATT ATAGGGCTTA CAGTATTGGA AGAGAACAGA ATCATTTATA TTTGAAAACA 
AAAGCAGCTG TAGTAACTTT ACAGTCAGCT TATCGTGGTA TGAAAGTGAG AAAAAGAATA 
AAGGATTGCA ACAAAGCAGC AGTCACTATA CAGTCTAAAT ACAGAGCTTA CAAAACCAAA 
AAGAAATATG CAACCTATAG AGCTTCAGCT ATTATAATTC AGAGATGGTA TCGAGGTATT 
AAAATTACAA ACCATCAGCA TAAGGAGTAT CTTAATTTGA AGAAGACAGC AATTAAAATC 
CAATCTGTTT ATAGAGGTAT TAGAGTTAGA AGACATATTC AACACATGCA CAGGGCAGCC 
ACTTTTATTA AAGCCATGTT TAAAATGCAT CAGTCAAGAA TAAGTTACCA TACAATGAGA 
AAAGCAGCTA TTGTTATTCA AGTAAGATGT AGAGCATATT ATCAAGGTAA AATGCAGCGT 
GAAAAGTACC TGACAATTTT GAAAGCTGTT AAAGTCCTTC AGGCAAGTTT TAGAGGAGTA 
AGAGTTAGAC GGACTCTTAG AAAGATGCAG ACTGCAGCAA CACTCATTCA GTCAAACTAC 
AGAAGATACA GACAGCAAAC ATACTTTAAT AAGTTAAAGA AAATAACAAA AACAGTACAG 
CAAAGATACT GGGCAATGAA AGAAAGAAAC ATACAATTTC AAAGGTATAA CAAACTGAGG 
CATTCTGTAA TATACATTCA GGCTATTTTT AGGGGAAAGA AAGCTAGAAG ACATTTAAAA 
ATGATGCATA TAGCCGCAAC TCTCATTCAG AGGAGATTTA GAACTCTAAT GATGAGAAGA 
AGATTCCTCT CTCTCAAGAA AACTGCTATT TTGATTCAGA GAAAATATCG GGCACATCTT 
TGTACAAAGC ATCACTTACA GTTCCTTCAG GTACAAAATG CAGTTATTAA AATCCAGTCA 
TCATACAGAA GATGGATGAT AAGGAAAAGG ATGCGAGAGA TGCACAGGGC TGCTACTTTC 
ATCCAGTCTA CTTTCAGAAT GCACAGATTA CATATGAGAT ATCAGGCTTT GAAACAGGCC 
TCCGTTGTGA TCCAACAGCA ATACCAAGCA AATAGAGCTG CAAAACTGCA GAGGCAGCAT 
TATCTCAGAC AAAGACACTC TGCTGTGATC CTTCAGGCTG CATTCAGGGG TATGAAAACT 
AGAAGACATT TGAAGAGTAT GCATTCCTCT GCAACCCTTA TTCAGAGTAG GTTTAGATCA 
TTACTGGTGA GGAGAAGATT CATTTCCCTC AAAAAAGCTA CTATTTTTGT TCAGAGGAAA 
TATCGAGCCA CCATTTGTGC CAAACATAAA TTGTACCAAT TCTTGCACTT AAGAAAGGCA 
GCCATTACAA TACAGTCATC TTACAGAAGA CTGATGGTAA AGAAGAAGTT ACAAGAAATG 
CAAAGGGCTG CAGTTCTCAT TCAGGCTACT TTCAGGATGT ACAGAACATA TATTACATTT 
CAGACTTGGA AACATGCTTC AATTCTAATT CAGCAACATT ATCGAACATA TAGAGCTGCA 
AAATTACAAA GAGAAAATTA TATCAGACAA TGGCATTCTG CTGTGGTTAT TCAGGCTGCA 
TATAAAGGAA TGAAAGCAAG ACAACTTTTA AGGGAAAAAC ACAAAGCTTC TATCGTAATA 
CAAAGCACCT ACAGAATGTA TAGGCAGTAT TGTTTCTACC AAAAGCTTCA GTGGGCTACA 
AAAATCATAC AAGAAAAATA TAGAGCAAAT AAAAAGAAAC AGAAAGTATT TCAACACAAT 
GAACTTAAGA AAGAGACTTG TGTTCAGGCA GGTTTTCAGG ACATGAACAT AAAAAAACAG 
ATTCAGGAAC AGCACCAGGC TGCCATTATT ATTCAGAAGC ATTGTAAAGC CTTTAAAATA 
AGGAAGCATT ATCTCCACCT TAGAGCAACA GTAGTTTCTA TTCAAAGAAG ATACAGAAAA 
CTAACTGCAG TGCGTACCCA AGCAGTTATT TGTATACAGT CTTATTACAG AGGCTTTAAA 
GTACGAAAGG ATATTCAAAA TATGCACCGG GCTGCCACAC TAATTCAGTC ATTCTATCGA 
ATGCACAGGG CCAAAGTTGA TTATGAAACA AAGAAAACTG CAATTGTGGT TATACAGAAT 
TATTATAGGT TGTATGTTAG AGTAAAAACA GAAAGAAAAA ACTTTTTAGC AGTTCAGAAA 
TCTGTACGAA CTATTCAGGC TGCTTTTAGA GGCATGAAAG TTAGACAAAA ATTGAAAAAT 
GTATCAGAGG AAAAGATGGC AGCCATTGTT AACCAATCTG CACTCTGCTG TTACAGAAGT 
AAAACTCAGT ATGAAGCTGT TCAAAGTGAA GGTGTTATGA TTCAAGAGTG GTATAAAGCT 
TCTGGCCTTG CTTGTTCACA GGAAGCAGAG TATCATTCTC AAAGTAGGGC TGCAGTAACA 
ATTCAAAAAG CTTTTTGTAG AATGGTCACA AGAAAACTGG AAACACAGAA ATGTGCTGCC 
CTACGGATTC AGTTCTTCCT TCAGATGGCT GTGTATCGGA GAAGATTTGT TCAGCAGAAA 
AGAGCTGCTA TCACTTTACA GCATTATTTT AGGACGTGGC AAACCAGAAA ACAGTTTTTA 
CTATATAGAA AAGCAGCAGT GGTTTTACAA AATCACTACA GAGCATTTCT GTCTGCAAAA 
CATCAAAGAC AAGTCTATTT ACAGATCAGA AGCAGTGTTA TCATTATTCA AGCTAGAAGT 
AAAGGATTTA TACAGAAACG GAAGTTTCAG GAAATTAAAA ATAGCACCAT AAAAATTCAG 
GCTATGTGGA GGAGATATAG AGCCAAGAAA TATTTATGTA AAGTGAAAGC TGCCTGCAAG 
ATTCAAGCCT GGTATAGATG TTGGAGAGCA CACAAAGAAT ATCTAGCTAT ATTAAAAGCT 
GTTAAAATTA TTCAAGGTTG CTTCTATACC AAACTAGAGA GAACACGGTT TTTGAATGTG 
AGAGCATCAG CAATTATCAT TCAGAGAAAA TGGAGAGCTA TACTTCCTGC AAAGATAGCT 
CATGAACACT TCTTAATGAT AAAAAGACAT CGAGCTGCTT GTTTGATCCA AGCACATTAT 
AGAGGATATA AAGGAAGGCA GGTCTTTCTT CGGCAGAAAT CTGCTGCTTT GATCATACAA 
AAATATATAC GAGCCAGGGA GGCTGGAAAG CATGAAAGGA TAAAATATAT TGAATTTAAA 
AAATCTACAG TTATCCTACA AGCACTGGTG CGTGGTTGGC TAGTACGAAA AAGATTTTTA 
GAACAGAGAG CCAAAATTCG ACTTCTTCAC TTCACTGCAG CTGCATATTA TCACCTGAAT 
GCTGTTAGAA TTCAAAGAGC CTATAAACTT TACCTGGCTG TGAAGAATGC TAACAAGCAG 
GTTAATTCAG TCATCTGTAT TCAGAGATGG TTTCGAGCAA GATTACAAGA AAAGAGATTT 
ATTCAGAAAT ATCATAGCAT CAAAAAGATT GAGCATGAAG GTCAAGAATG TCTGAGCCAG 
CGAAATAGGG CTGCATCAGT AATACAGAAA GCAGTGCGCC ATTTTCTCCT CCGTAAAAAG 
CAGGAAAAAT TCACTAGTGG AATCATTAAA ATTCAGGCAT TATGGAGAGG CTATTCTTGG 
AGGAAGAAAA ATGATTGTAC AAAAATTAAA GCTATACGAC TAAGTCTTCA AGTTGTTAAT 
AGGGAGATTC GAGAAGAAAA CAAACTCTAC AAAAGAACTG CACTTGCACT TCATTACCTT 
TTGACATATA AGCACCTTTC TGCCATTCTT GAGGCCTTAA AACACCTAGA GGTAGTTACT 
AGATTGTCTC CACTTTGTTG TGAGAACATG GCCCAGAGTG GAGCAATTTC TAAAATATTT 
GTTTTGATCC GAAGTTGTAA TCGCAGTATT CCTTGTATGG AAGTCATCAG ATATGCTGTG 
CAAGTCTTGC TTAATGTATC TAAGTATGAG AAAACTACTT CAGCAGTTTA TGATGTAGAA 
AATTGTATAG ATATACTATT GGAGCTTTTG CAGATATACC GAGAAAAGCC TGGTAATAAA 
GTTGCAGACA AAGGCGGAAG CATTTTTACA AAAACTTGTT GTTTGTTGGC TATTTTACTG 
AAGACAACAA ATAGAGCCTC TGATGTACGA AGTAGGTCCA AAGTTGTTGA CCGTATTTAC 
AGTCTCTACA AACTTACAGC TCATAAACAT AAAATGAATA CTGAAAGAAT ACTTTACAAG 
CAAAAGAAGA ATTCTTCTAT AAGCATTCCT TTTATCCCAG AAACACCTGT AAGGACCAGA 
ATAGTTTCAA GACTTAAGCC AGATTGGGTT TTGAGAAGAG ATAACATGGA AGAAATCACA 
AATCCCCTGC AAGCTATTCA AATGGTGATG GATACGCTTG GCATTCCTTA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASPM ( NM_001206846.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001193775.1 Homo sapiens assembly factor for spindle microtubules (ASPM), transcript variant 2, mRNA.

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CloneID	OHu23051
Clone ID Related Accession (Same CDS sequence)	NM_001206846.1
Accession Version	NM_001206846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Homo sapiens(human)
Product	abnormal spindle-like microcephaly-associated protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK125107.1, AK226178.1, AL353809.20, BC034607.1 and BX648804.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK226178.1, AY971956.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAACC GGCGAGTGGG GCGAGGCTGC TGGGAAGTGA GCCCGACCGA GCGGAGGCCG 
CCCGCGGGGC TGCGGGGCCC CGCGGCCGAG GAGGAGGCGT CTTCCCCGCC GGTCCTGTCT 
CTCAGCCACT TCTGCAGGTC TCCTTTCCTT TGCTTCGGGG ACGTTCTCCT GGGAGCCTCA 
CGGACGCTGT CTCTGGCCCT AGACAACCCT AACGAGGAGG TGGCAGAAGT GAAGATCTCC 
CACTTCCCGG CCGCGGACCT GGGCTTCAGT GTGTCGCAGC GCTGTTTCGT GTTGCAGCCT 
AAAGAGAAAA TTGTTATTTC TGTTAACTGG ACACCACTCA AAGAAGGCCG AGTAAGAGAG 
ATTATGACAT TTCTTGTAAA TGATGTTCTG AAACACCAAG CTATATTACT AGGAAATGCA 
GAAGAGCAGA AAAAGAAAAA GAGGAGTCTT TGGGATACCA TTAAAAAGAA GAAAATTTCA 
GCCTCTACAA GTCACAACAG AAGGGTTTCA AATATTCAGA ATGTTAATAA AACATTTAGT 
GTTTCCCAAA AAGTTGACAG AGTTAGGAGC CCACTACAAG CTTGTGAAAA CTTGGCTATG 
AATGAAGGCG GTCCCCCAAC AGAAAACAAT TCTTTAATAC TTGAAGAAAA TAAAATACCC 
ATATCACCTA TTAGCCCTGC TTTCAATGAA TGCCATGGTG CAACTTGCTT GCCACTCTCT 
GTACGTCGAT CTACTACCTA CTCATCTCTT CATGCATCAG AAAATAGGGA ACTATTAAAT 
GTACACAGTG CCAACGTTTC AAAAGTTTCT TTTAATGAGA AAGCTGTAAC TGAAACTTCC 
TTTAATTCCG TAAATGTTAA TGGCCAAAGA GGAGAGAATA GTAAACTTAG TCTTACCCCC 
AACTGTTCTT CAACTTTGAA CATTACACAA AGCCAAATAC ATTTTCTAAG TCCAGATTCT 
TTTGTAAATA ATAGTCATGG AGCTAATAAT GAACTAGAAT TAGTAACATG TCTTTCATCA 
GATATGTTTA TGAAAGATAA TTCACAGCCT GTGCATTTGG AATCAACAAT TGCACATGAA 
ATTTATCAGA AAATTTTAAG TCCAGATTCT TTCATAAAAG ATAATTATGG ACTAAATCAG 
GATCTAGAAT CAGAGTCAGT TAATCCTATT TTATCCCCTA ATCAATTTTT AAAAGATAAC 
ATGGCATATA TGTGTACATC TCAGCAAACA TGTAAAGTAC CATTATCAAA TGAAAATTCT 
CAAGTCCCAC AGTCTCCTGA AGATTGGAGA AAAAGTGAAG TTTCGCCACG TATTCCTGAA 
TGTCAGGGTT CAAAATCTCC CAAAGCTATT TTTGAAGAAC TAGTAGAAAT GAAGTCAAAT 
TACTACAGTT TTATAAAACA AAATAATCCT AAATTTTCTG CAGTTCAGGA TATTTCTAGT 
CATAGCCACA ATAAACAACC TAAGAGACGT CCAATACTTT CTGCCACTGT TACTAAAAGG 
AAGGCCACCT GTACCAGAGA AAACCAAACT GAGATTAATA AACCAAAAGC AAAAAGATGT 
CTCAACAGTG CAGTGGGTGA ACATGAAAAA GTAATAAATA ATCAAAAGGA AAAAGAAGAT 
TTTCATTCTT ATCTTCCAAT TATAGATCCA ATATTAAGTA AATCTAAGAG TTATAAAAAC 
GAGGTAACAC CCTCTTCGAC AACAGCTTCA GTTGCTCGGA AAAGAAAGAG CGATGGAAGC 
ATGGAAGATG CAAATGTGAG AGTTGCAATT ACAGAACATA CAGAAGTGCG AGAAATCAAA 
AGAATCCATT TTTCTCCCTC AGAGCCTAAA ACATCAGCTG TTAAGAAAAC AAAAAATGTG 
ACAACACCCA TCTCAAAACG TATTAGCAAC AGAGAGAAAT TAAACCTGAA GAAGAAAACT 
GATTTATCAA TATTCAGAAC TCCAATTTCT AAAACAAACA AAAGGACAAA ACCCATTATC 
GCTGTGGCAC AGTCCAGTTT GACCTTCATA AAACCATTAA AAACAGATAT TCCCAGACAC 
CCGATGCCAT TTGCTGCAAA AAACATGTTT TATGATGAAC GCTGGAAGGA AAAGCAGGAA 
CAGGGCTTCA CTTGGTGGTT AAATTTTATA TTAACCCCTG ATGACTTCAC TGTAAAAACA 
AATATTTCTG AAGTAAATGC TGCTACTCTT CTTTTGGGAA TAGAGAATCA ACATAAAATA 
AGTGTTCCTA GAGCACCTAC AAAAGAGGAA ATGTCTCTCA GAGCTTATAC TGCTCGGTGT 
AGGTTAAACA GACTACGTCG TGCAGCATGC CGTTTGTTTA CTTCTGAAAA AATGGTTAAA 
GCTATTAAAA AGCTTGAAAT TGAAATTGAA GCTAGGCGGT TAATTGTTCG AAAAGATAGA 
CACCTATGGA AAGATGTGGG AGAACGTCAG AAAGTCCTGA ATTGGCTGTT GTCCTACAAT 
CCTTTGTGGC TTCGAATTGG TCTAGAGACA ACTTATGGAG AACTCATATC TTTGGAAGAT 
AACAGTGATG TCACAGGGTT GGCTATGTTT ATTCTGAATC GCCTACTTTG GAATCCTGAT 
ATAGCAGCTG AGTATAGACA CCCCACTGTT CCTCACCTGT ATAGAGATGG TCATGAAGAA 
GCTTTGTCCA AGTTTACATT GAAAAAGTTA TTGTTGTTGG TCTGTTTTCT TGATTATGCT 
AAAATTTCCA GACTCATTGA TCATGATCCT TGTCTCTTCT GTAAAGATGC CGAATTCAAG 
GCTAGTAAAG AAATCCTTTT GGCTTTTTCA CGAGATTTCC TAAGTGGTGA AGGTGACCTT 
TCCCGTCACC TTGGCTTATT GGGATTACCT GTTAACCATG TTCAGACACC ATTTGATGAA 
TTTGATTTTG CCGTTACAAA TCTTGCCGTA GACTTGCAAT GTGGAGTGCG CCTTGTGCGA 
ACCATGGAAC TTCTCACACA GAACTGGGAC CTCTCAAAGA AACTCAGGAT TCCGGCAATA 
AGTCGTCTTC AAAAGATGCA CAATGTTGAC ATTGTTCTTC AAGTTCTTAA ATCACGAGGA 
ATTGAATTAA GTGATGAGCA TGGAAATACA ATTCTATCTA AGGATATTGT GGATAGGCAC 
AGAGAAAAAA CTCTCAGGTT GCTTTGGAAA ATAGCGTTTG CTTTTCAGGT GGATATTTCC 
CTTAACTTAG ATCAATTAAA GGAAGAAATT GCCTTTCTAA AACACACAAA GAGTATAAAG 
AAAACAATAT CTCTACTATC ATGCCATTCT GATGATCTTA TTAATAAGAA AAAAGGCAAA 
AGGGATAGTG GTTCCTTTGA ACAATATAGT GAAAACATAA AGTTATTGAT GGATTGGGTA 
AATGCTGTTT GTGCCTTCTA TAATAAAAAG GTGGAGAATT TTACAGTGTC TTTCTCAGAC 
GGCCGTGTGT TATGTTACCT GATCCACCAT TACCATCCTT GCTATGTGCC ATTTGACGCT 
ATATGTCAGC GTACTACTCA AACTGTGGAA TGTACGCAAA CTGGTTCAGT GGTATTAAAT 
TCATCATCTG AATCTGATGA CAGTTCTCTG GATATGTCTC TTAAAGCATT TGATCATGAA 
AATACTTCAG AGCTATACAA AGAGCTCCTA GAAAATGAAA AGAAAAATTT TCACTTGGTT 
AGGTCTGCAG TTAGAGACCT TGGTGGAATA CCTGCTATGA TTAATCATTC AGATATGTCA 
AATACAATTC CAGATGAAAA GGTGGTTATT ACCTATTTGT CATTTCTTTG TGCAAGGCTT 
TTGGATCTTC GTAAAGAAAT AAGAGCTGCT CGACTCATAC AAACAACATG GAGAAAATAT 
AAACTAAAAA CAGATCTCAA ACGCCATCAG GAGAGAGAGA AAGCTGCAAG AATTATTCAA 
TTGGCTGTAA TCAATTTTCT AGCAAAACAA AGATTGAGAA AAAGAGTTAA TGCAGCACTC 
GTCATTCAGA AATATTGGCG AAGAGTCTTA GCACAGAGAA AATTATTAAT GTTAAAAAAG 
GAAAAGCTGG AAAAAGTTCA AAATAAAGCA GCATCACTTA TTCAGGCTAT GTGGAGGAGA 
TATAGAGCCA AGAAATATTT ATGTAAAGTG AAAGCTGCCT GCAAGATTCA AGCCTGGTAT 
AGATGTTGGA GAGCACACAA AGAATATCTA GCTATATTAA AAGCTGTTAA AATTATTCAA 
GGTTGCTTCT ATACCAAACT AGAGAGAACA CGGTTTTTGA ATGTGAGAGC ATCAGCAATT 
ATCATTCAGA GAAAATGGAG AGCTATACTT CCTGCAAAGA TAGCTCATGA ACACTTCTTA 
ATGATAAAAA GACATCGAGC TGCTTGTTTG ATCCAAGCAC ATTATAGAGG ATATAAAGGA 
AGGCAGGTCT TTCTTCGGCA GAAATCTGCT GCTTTGATCA TACAAAAATA TATACGAGCC 
AGGGAGGCTG GAAAGCATGA AAGGATAAAA TATATTGAAT TTAAAAAATC TACAGTTATC 
CTACAAGCAC TGGTGCGTGG TTGGCTAGTA CGAAAAAGAT TTTTAGAACA GAGAGCCAAA 
ATTCGACTTC TTCACTTCAC TGCAGCTGCA TATTATCACC TGAATGCTGT TAGAATTCAA 
AGAGCCTATA AACTTTACCT GGCTGTGAAG AATGCTAACA AGCAGGTTAA TTCAGTCATC 
TGTATTCAGA GATGGTTTCG AGCAAGATTA CAAGAAAAGA GATTTATTCA GAAATATCAT 
AGCATCAAAA AGATTGAGCA TGAAGGTCAA GAATGTCTGA GCCAGCGAAA TAGGGCTGCA 
TCAGTAATAC AGAAAGCAGT GCGCCATTTT CTCCTCCGTA AAAAGCAGGA AAAATTCACT 
AGTGGAATCA TTAAAATTCA GGCATTATGG AGAGGCTATT CTTGGAGGAA GAAAAATGAT 
TGTACAAAAA TTAAAGCTAT ACGACTAAGT CTTCAAGTTG TTAATAGGGA GATTCGAGAA 
GAAAACAAAC TCTACAAAAG AACTGCACTT GCACTTCATT ACCTTTTGAC ATATAAGCAC 
CTTTCTGCCA TTCTTGAGGC CTTAAAACAC CTAGAGGTAG TTACTAGATT GTCTCCACTT 
TGTTGTGAGA ACATGGCCCA GAGTGGAGCA ATTTCTAAAA TATTTGTTTT GATCCGAAGT 
TGTAATCGCA GTATTCCTTG TATGGAAGTC ATCAGATATG CTGTGCAAGT CTTGCTTAAT 
GTATCTAAGT ATGAGAAAAC TACTTCAGCA GTTTATGATG TAGAAAATTG TATAGATATA 
CTATTGGAGC TTTTGCAGAT ATACCGAGAA AAGCCTGGTA ATAAAGTTGC AGACAAAGGC 
GGAAGCATTT TTACAAAAAC TTGTTGTTTG TTGGCTATTT TACTGAAGAC AACAAATAGA 
GCCTCTGATG TACGAAGTAG GTCCAAAGTT GTTGACCGTA TTTACAGTCT CTACAAACTT 
ACAGCTCATA AACATAAAAT GAATACTGAA AGAATACTTT ACAAGCAAAA GAAGAATTCT 
TCTATAAGCA TTCCTTTTAT CCCAGAAACA CCTGTAAGGA CCAGAATAGT TTCAAGACTT 
AAGCCAGATT GGGTTTTGAG AAGAGATAAC ATGGAAGAAA TCACAAATCC CCTGCAAGCT 
ATTCAAATGG TGATGGATAC GCTTGGCATT CCTTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193775.1
CDS	258..5936
Translation	MANRRVGRGCWEVSPTERRPPAGLRGPAAEEEASSPPVLSLSHFCRSPFLCFGDVLLGASRTLSLALDNPNEEVAEVKISHFPAADLGFSVSQRCFVLQPKEKIVISVNWTPLKEGRVREIMTFLVNDVLKHQAILLGNAEEQKKKKRSLWDTIKKKKISASTSHNRRVSNIQNVNKTFSVSQKVDRVRSPLQACENLAMNEGGPPTENNSLILEENKIPISPISPAFNECHGATCLPLSVRRSTTYSSLHASENRELLNVHSANVSKVSFNEKAVTETSFNSVNVNGQRGENSKLSLTPNCSSTLNITQSQIHFLSPDSFVNNSHGANNELELVTCLSSDMFMKDNSQPVHLESTIAHEIYQKILSPDSFIKDNYGLNQDLESESVNPILSPNQFLKDNMAYMCTSQQTCKVPLSNENSQVPQSPEDWRKSEVSPRIPECQGSKSPKAIFEELVEMKSNYYSFIKQNNPKFSAVQDISSHSHNKQPKRRPILSATVTKRKATCTRENQTEINKPKAKRCLNSAVGEHEKVINNQKEKEDFHSYLPIIDPILSKSKSYKNEVTPSSTTASVARKRKSDGSMEDANVRVAITEHTEVREIKRIHFSPSEPKTSAVKKTKNVTTPISKRISNREKLNLKKKTDLSIFRTPISKTNKRTKPIIAVAQSSLTFIKPLKTDIPRHPMPFAAKNMFYDERWKEKQEQGFTWWLNFILTPDDFTVKTNISEVNAATLLLGIENQHKISVPRAPTKEEMSLRAYTARCRLNRLRRAACRLFTSEKMVKAIKKLEIEIEARRLIVRKDRHLWKDVGERQKVLNWLLSYNPLWLRIGLETTYGELISLEDNSDVTGLAMFILNRLLWNPDIAAEYRHPTVPHLYRDGHEEALSKFTLKKLLLLVCFLDYAKISRLIDHDPCLFCKDAEFKASKEILLAFSRDFLSGEGDLSRHLGLLGLPVNHVQTPFDEFDFAVTNLAVDLQCGVRLVRTMELLTQNWDLSKKLRIPAISRLQKMHNVDIVLQVLKSRGIELSDEHGNTILSKDIVDRHREKTLRLLWKIAFAFQVDISLNLDQLKEEIAFLKHTKSIKKTISLLSCHSDDLINKKKGKRDSGSFEQYSENIKLLMDWVNAVCAFYNKKVENFTVSFSDGRVLCYLIHHYHPCYVPFDAICQRTTQTVECTQTGSVVLNSSSESDDSSLDMSLKAFDHENTSELYKELLENEKKNFHLVRSAVRDLGGIPAMINHSDMSNTIPDEKVVITYLSFLCARLLDLRKEIRAARLIQTTWRKYKLKTDLKRHQEREKAARIIQLAVINFLAKQRLRKRVNAALVIQKYWRRVLAQRKLLMLKKEKLEKVQNKAASLIQAMWRRYRAKKYLCKVKAACKIQAWYRCWRAHKEYLAILKAVKIIQGCFYTKLERTRFLNVRASAIIIQRKWRAILPAKIAHEHFLMIKRHRAACLIQAHYRGYKGRQVFLRQKSAALIIQKYIRAREAGKHERIKYIEFKKSTVILQALVRGWLVRKRFLEQRAKIRLLHFTAAAYYHLNAVRIQRAYKLYLAVKNANKQVNSVICIQRWFRARLQEKRFIQKYHSIKKIEHEGQECLSQRNRAASVIQKAVRHFLLRKKQEKFTSGIIKIQALWRGYSWRKKNDCTKIKAIRLSLQVVNREIREENKLYKRTALALHYLLTYKHLSAILEALKHLEVVTRLSPLCCENMAQSGAISKIFVLIRSCNRSIPCMEVIRYAVQVLLNVSKYEKTTSAVYDVENCIDILLELLQIYREKPGNKVADKGGSIFTKTCCLLAILLKTTNRASDVRSRSKVVDRIYSLYKLTAHKHKMNTERILYKQKKNSSISIPFIPETPVRTRIVSRLKPDWVLRRDNMEEITNPLQAIQMVMDTLGIPY

Target ORF information:

RefSeq Version	NM_001206846.1
Organism	Homo sapiens(human)
Definition	Homo sapiens assembly factor for spindle microtubules (ASPM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206846.1

ORF Insert Sequence:

ATGGCGAACC GGCGAGTGGG GCGAGGCTGC TGGGAAGTGA GCCCGACCGA GCGGAGGCCG 
CCCGCGGGGC TGCGGGGCCC CGCGGCCGAG GAGGAGGCGT CTTCCCCGCC GGTCCTGTCT 
CTCAGCCACT TCTGCAGGTC TCCTTTCCTT TGCTTCGGGG ACGTTCTCCT GGGAGCCTCA 
CGGACGCTGT CTCTGGCCCT AGACAACCCT AACGAGGAGG TGGCAGAAGT GAAGATCTCC 
CACTTCCCGG CCGCGGACCT GGGCTTCAGT GTGTCGCAGC GCTGTTTCGT GTTGCAGCCT 
AAAGAGAAAA TTGTTATTTC TGTTAACTGG ACACCACTCA AAGAAGGCCG AGTAAGAGAG 
ATTATGACAT TTCTTGTAAA TGATGTTCTG AAACACCAAG CTATATTACT AGGAAATGCA 
GAAGAGCAGA AAAAGAAAAA GAGGAGTCTT TGGGATACCA TTAAAAAGAA GAAAATTTCA 
GCCTCTACAA GTCACAACAG AAGGGTTTCA AATATTCAGA ATGTTAATAA AACATTTAGT 
GTTTCCCAAA AAGTTGACAG AGTTAGGAGC CCACTACAAG CTTGTGAAAA CTTGGCTATG 
AATGAAGGCG GTCCCCCAAC AGAAAACAAT TCTTTAATAC TTGAAGAAAA TAAAATACCC 
ATATCACCTA TTAGCCCTGC TTTCAATGAA TGCCATGGTG CAACTTGCTT GCCACTCTCT 
GTACGTCGAT CTACTACCTA CTCATCTCTT CATGCATCAG AAAATAGGGA ACTATTAAAT 
GTACACAGTG CCAACGTTTC AAAAGTTTCT TTTAATGAGA AAGCTGTAAC TGAAACTTCC 
TTTAATTCCG TAAATGTTAA TGGCCAAAGA GGAGAGAATA GTAAACTTAG TCTTACCCCC 
AACTGTTCTT CAACTTTGAA CATTACACAA AGCCAAATAC ATTTTCTAAG TCCAGATTCT 
TTTGTAAATA ATAGTCATGG AGCTAATAAT GAACTAGAAT TAGTAACATG TCTTTCATCA 
GATATGTTTA TGAAAGATAA TTCACAGCCT GTGCATTTGG AATCAACAAT TGCACATGAA 
ATTTATCAGA AAATTTTAAG TCCAGATTCT TTCATAAAAG ATAATTATGG ACTAAATCAG 
GATCTAGAAT CAGAGTCAGT TAATCCTATT TTATCCCCTA ATCAATTTTT AAAAGATAAC 
ATGGCATATA TGTGTACATC TCAGCAAACA TGTAAAGTAC CATTATCAAA TGAAAATTCT 
CAAGTCCCAC AGTCTCCTGA AGATTGGAGA AAAAGTGAAG TTTCGCCACG TATTCCTGAA 
TGTCAGGGTT CAAAATCTCC CAAAGCTATT TTTGAAGAAC TAGTAGAAAT GAAGTCAAAT 
TACTACAGTT TTATAAAACA AAATAATCCT AAATTTTCTG CAGTTCAGGA TATTTCTAGT 
CATAGCCACA ATAAACAACC TAAGAGACGT CCAATACTTT CTGCCACTGT TACTAAAAGG 
AAGGCCACCT GTACCAGAGA AAACCAAACT GAGATTAATA AACCAAAAGC AAAAAGATGT 
CTCAACAGTG CAGTGGGTGA ACATGAAAAA GTAATAAATA ATCAAAAGGA AAAAGAAGAT 
TTTCATTCTT ATCTTCCAAT TATAGATCCA ATATTAAGTA AATCTAAGAG TTATAAAAAC 
GAGGTAACAC CCTCTTCGAC AACAGCTTCA GTTGCTCGGA AAAGAAAGAG CGATGGAAGC 
ATGGAAGATG CAAATGTGAG AGTTGCAATT ACAGAACATA CAGAAGTGCG AGAAATCAAA 
AGAATCCATT TTTCTCCCTC AGAGCCTAAA ACATCAGCTG TTAAGAAAAC AAAAAATGTG 
ACAACACCCA TCTCAAAACG TATTAGCAAC AGAGAGAAAT TAAACCTGAA GAAGAAAACT 
GATTTATCAA TATTCAGAAC TCCAATTTCT AAAACAAACA AAAGGACAAA ACCCATTATC 
GCTGTGGCAC AGTCCAGTTT GACCTTCATA AAACCATTAA AAACAGATAT TCCCAGACAC 
CCGATGCCAT TTGCTGCAAA AAACATGTTT TATGATGAAC GCTGGAAGGA AAAGCAGGAA 
CAGGGCTTCA CTTGGTGGTT AAATTTTATA TTAACCCCTG ATGACTTCAC TGTAAAAACA 
AATATTTCTG AAGTAAATGC TGCTACTCTT CTTTTGGGAA TAGAGAATCA ACATAAAATA 
AGTGTTCCTA GAGCACCTAC AAAAGAGGAA ATGTCTCTCA GAGCTTATAC TGCTCGGTGT 
AGGTTAAACA GACTACGTCG TGCAGCATGC CGTTTGTTTA CTTCTGAAAA AATGGTTAAA 
GCTATTAAAA AGCTTGAAAT TGAAATTGAA GCTAGGCGGT TAATTGTTCG AAAAGATAGA 
CACCTATGGA AAGATGTGGG AGAACGTCAG AAAGTCCTGA ATTGGCTGTT GTCCTACAAT 
CCTTTGTGGC TTCGAATTGG TCTAGAGACA ACTTATGGAG AACTCATATC TTTGGAAGAT 
AACAGTGATG TCACAGGGTT GGCTATGTTT ATTCTGAATC GCCTACTTTG GAATCCTGAT 
ATAGCAGCTG AGTATAGACA CCCCACTGTT CCTCACCTGT ATAGAGATGG TCATGAAGAA 
GCTTTGTCCA AGTTTACATT GAAAAAGTTA TTGTTGTTGG TCTGTTTTCT TGATTATGCT 
AAAATTTCCA GACTCATTGA TCATGATCCT TGTCTCTTCT GTAAAGATGC CGAATTCAAG 
GCTAGTAAAG AAATCCTTTT GGCTTTTTCA CGAGATTTCC TAAGTGGTGA AGGTGACCTT 
TCCCGTCACC TTGGCTTATT GGGATTACCT GTTAACCATG TTCAGACACC ATTTGATGAA 
TTTGATTTTG CCGTTACAAA TCTTGCCGTA GACTTGCAAT GTGGAGTGCG CCTTGTGCGA 
ACCATGGAAC TTCTCACACA GAACTGGGAC CTCTCAAAGA AACTCAGGAT TCCGGCAATA 
AGTCGTCTTC AAAAGATGCA CAATGTTGAC ATTGTTCTTC AAGTTCTTAA ATCACGAGGA 
ATTGAATTAA GTGATGAGCA TGGAAATACA ATTCTATCTA AGGATATTGT GGATAGGCAC 
AGAGAAAAAA CTCTCAGGTT GCTTTGGAAA ATAGCGTTTG CTTTTCAGGT GGATATTTCC 
CTTAACTTAG ATCAATTAAA GGAAGAAATT GCCTTTCTAA AACACACAAA GAGTATAAAG 
AAAACAATAT CTCTACTATC ATGCCATTCT GATGATCTTA TTAATAAGAA AAAAGGCAAA 
AGGGATAGTG GTTCCTTTGA ACAATATAGT GAAAACATAA AGTTATTGAT GGATTGGGTA 
AATGCTGTTT GTGCCTTCTA TAATAAAAAG GTGGAGAATT TTACAGTGTC TTTCTCAGAC 
GGCCGTGTGT TATGTTACCT GATCCACCAT TACCATCCTT GCTATGTGCC ATTTGACGCT 
ATATGTCAGC GTACTACTCA AACTGTGGAA TGTACGCAAA CTGGTTCAGT GGTATTAAAT 
TCATCATCTG AATCTGATGA CAGTTCTCTG GATATGTCTC TTAAAGCATT TGATCATGAA 
AATACTTCAG AGCTATACAA AGAGCTCCTA GAAAATGAAA AGAAAAATTT TCACTTGGTT 
AGGTCTGCAG TTAGAGACCT TGGTGGAATA CCTGCTATGA TTAATCATTC AGATATGTCA 
AATACAATTC CAGATGAAAA GGTGGTTATT ACCTATTTGT CATTTCTTTG TGCAAGGCTT 
TTGGATCTTC GTAAAGAAAT AAGAGCTGCT CGACTCATAC AAACAACATG GAGAAAATAT 
AAACTAAAAA CAGATCTCAA ACGCCATCAG GAGAGAGAGA AAGCTGCAAG AATTATTCAA 
TTGGCTGTAA TCAATTTTCT AGCAAAACAA AGATTGAGAA AAAGAGTTAA TGCAGCACTC 
GTCATTCAGA AATATTGGCG AAGAGTCTTA GCACAGAGAA AATTATTAAT GTTAAAAAAG 
GAAAAGCTGG AAAAAGTTCA AAATAAAGCA GCATCACTTA TTCAGGCTAT GTGGAGGAGA 
TATAGAGCCA AGAAATATTT ATGTAAAGTG AAAGCTGCCT GCAAGATTCA AGCCTGGTAT 
AGATGTTGGA GAGCACACAA AGAATATCTA GCTATATTAA AAGCTGTTAA AATTATTCAA 
GGTTGCTTCT ATACCAAACT AGAGAGAACA CGGTTTTTGA ATGTGAGAGC ATCAGCAATT 
ATCATTCAGA GAAAATGGAG AGCTATACTT CCTGCAAAGA TAGCTCATGA ACACTTCTTA 
ATGATAAAAA GACATCGAGC TGCTTGTTTG ATCCAAGCAC ATTATAGAGG ATATAAAGGA 
AGGCAGGTCT TTCTTCGGCA GAAATCTGCT GCTTTGATCA TACAAAAATA TATACGAGCC 
AGGGAGGCTG GAAAGCATGA AAGGATAAAA TATATTGAAT TTAAAAAATC TACAGTTATC 
CTACAAGCAC TGGTGCGTGG TTGGCTAGTA CGAAAAAGAT TTTTAGAACA GAGAGCCAAA 
ATTCGACTTC TTCACTTCAC TGCAGCTGCA TATTATCACC TGAATGCTGT TAGAATTCAA 
AGAGCCTATA AACTTTACCT GGCTGTGAAG AATGCTAACA AGCAGGTTAA TTCAGTCATC 
TGTATTCAGA GATGGTTTCG AGCAAGATTA CAAGAAAAGA GATTTATTCA GAAATATCAT 
AGCATCAAAA AGATTGAGCA TGAAGGTCAA GAATGTCTGA GCCAGCGAAA TAGGGCTGCA 
TCAGTAATAC AGAAAGCAGT GCGCCATTTT CTCCTCCGTA AAAAGCAGGA AAAATTCACT 
AGTGGAATCA TTAAAATTCA GGCATTATGG AGAGGCTATT CTTGGAGGAA GAAAAATGAT 
TGTACAAAAA TTAAAGCTAT ACGACTAAGT CTTCAAGTTG TTAATAGGGA GATTCGAGAA 
GAAAACAAAC TCTACAAAAG AACTGCACTT GCACTTCATT ACCTTTTGAC ATATAAGCAC 
CTTTCTGCCA TTCTTGAGGC CTTAAAACAC CTAGAGGTAG TTACTAGATT GTCTCCACTT 
TGTTGTGAGA ACATGGCCCA GAGTGGAGCA ATTTCTAAAA TATTTGTTTT GATCCGAAGT 
TGTAATCGCA GTATTCCTTG TATGGAAGTC ATCAGATATG CTGTGCAAGT CTTGCTTAAT 
GTATCTAAGT ATGAGAAAAC TACTTCAGCA GTTTATGATG TAGAAAATTG TATAGATATA 
CTATTGGAGC TTTTGCAGAT ATACCGAGAA AAGCCTGGTA ATAAAGTTGC AGACAAAGGC 
GGAAGCATTT TTACAAAAAC TTGTTGTTTG TTGGCTATTT TACTGAAGAC AACAAATAGA 
GCCTCTGATG TACGAAGTAG GTCCAAAGTT GTTGACCGTA TTTACAGTCT CTACAAACTT 
ACAGCTCATA AACATAAAAT GAATACTGAA AGAATACTTT ACAAGCAAAA GAAGAATTCT 
TCTATAAGCA TTCCTTTTAT CCCAGAAACA CCTGTAAGGA CCAGAATAGT TTCAAGACTT 
AAGCCAGATT GGGTTTTGAG AAGAGATAAC ATGGAAGAAA TCACAAATCC CCTGCAAGCT 
ATTCAAATGG TGATGGATAC GCTTGGCATT CCTTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Autosomal recessive primary microcephaly due to ASPM mutations: An update.
Human mutation(2017 Dec)
L?tard P,Drunat S,Vial Y,Duerinckx S,Ernault A,Amram D,Arpin S,Bertoli M,Busa T,Ceulemans B,Desir J,Doco-Fenzy M,Elalaoui SC,Devriendt K,Faivre L,Francannet C,Genevi?ve D,G?rard M,Gitiaux C,Julia S,Lebon S,Lubala T,Mathieu-Dramard M,Maurey H,Metreau J,Nasserereddine S,Nizon M,Pierquin G,Pouvreau N,Rivier-Ringenbach C,Rossi M,Schaefer E,Sefiani A,Sigaudy S,Sznajer Y,Tunca Y,Guilmin Crepon S,Alberti C,Elmaleh-Berg?s M,Benzacken B,Wollnick B,Woods CG,Rauch A,Abramowicz M,El Ghouzzi V,Gressens P,Verloes A,Passemard S

Recapitulating cortical development with organoid culture in vitro and modeling abnormal spindle-like (ASPM related primary) microcephaly disease.
Protein & cell8(11)823-833(2017 Nov)
Li R,Sun L,Fang A,Li P,Wu Q,Wang X

Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2.
Journal of cell science130(21)3676-3684(2017 Nov)
Tungadi EA,Ito A,Kiyomitsu T,Goshima G