ORF » Species Summary » Homo sapiens » CRB1 cDNA ORF clone

CRB1 cDNA ORF clone, Homo sapiens(human)

The following CRB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu66169 XM_011509369.2
Latest version!		 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $475.30
$679.00
OHu22808 NM_001257966.1
Latest version!		 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu19399 NM_201253.2
Latest version!		 Homo sapiens crumbs 1, cell polarity complex component (CRB1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $727.30
$1039.00
OHu107688 NM_201253.3
Latest version!		 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu108189 NM_001193640.2
Latest version!		 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu108193 NM_001257965.2
Latest version!		 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu87140 XM_017000851.1
Latest version!		 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu87141 XM_017000852.1
Latest version!		 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu17475 NM_001193640.1
Latest version!		 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu66167 XM_011509367.1
Latest version!		 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu22677 NM_001257965.1
Latest version!		 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu66165 XM_011509365.2
Latest version!		 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CRB1 ( XM_011509369.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507671.1 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OHu66169
    Clone ID Related Accession (Same CDS sequence) XM_011509369.2
    Accession Version XM_011509369.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2664bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509369.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCTCTTC TACTTTTCCG AAGCAACAGG GATGTGTTTG TGAAGCTGGA GCTGCTAAGT 
    GGCTACATTC ACTTATCAAT TCAGGTCAAT AATCAGTCAA AGGTGCTTCT GTTCATTTCC 
    CACAACACCA GCGATGGAGA GTGGCATTTC GTGGAGGTAA TATTTGCAGA GGCTGTGACC 
    CTTACCTTAA TCGACGACTC CTGTAAGGAG AAATGCATCG CGAAAGCTCC TACTCCACTT 
    GAAAGTGATC AATCAATATG TGCTTTTCAG AACTCCTTTT TGGGTGGTTT ACCAGTGGGA 
    ATGACCAGCA ATGGTGTTGC TCTGCTTAAC TTCTATAATA TGCCATCCAC ACCTTCGTTT 
    GTAGGCTGTC TCCAAGACAT TAAAATTGAT TGGAATCACA TTACCCTGGA GAACATCTCG 
    TCTGGCTCAT CATTAAATGT CAAGGCAGGC TGTGTGAGAA AGGATTGGTG TGAAAGCCAA 
    CCTTGTCAAA GCAGAGGACG CTGCATCAAC TTGTGGCTGA GTTACCAGTG TGACTGCCAC 
    AGGCCCTATG AAGGCCCCAA CTGTCTGAGA GAGTATGTGG CAGGCAGATT TGGCCAGGAT 
    GACTCCACTG GTTATGTCAT CTTTACTCTT GATGAGAGCT ATGGAGACAC CATCAGCCTC 
    TCCATGTTTG TCCGAACGCT TCAACCATCA GGCTTACTTC TAGCTTTGGA AAACAGCACT 
    TATCAATATA TCCGTGTCTG GCTAGAGCGC GGCAGACTAG CAATGCTGAC TCCAAACTCT 
    CCCAAATTAG TAGTAAAATT TGTTCTTAAT GATGGAAATG TCCACTTGAT ATCTTTGAAA 
    ATCAAGCCAT ATAAAATTGA ACTGTATCAG TCTTCACAAA ACCTAGGATT TATTTCTGCT 
    TCTACGTGGA AAATCGAAAA GGGAGATGTC ATCTACATTG GTGGCCTACC TGACAAGCAA 
    GAGACTGAAC TTAATGGTGG ATTCTTCAAA GGCTGTATCC AAGATGTAAG ACTAAACAAC 
    CAAAATCTGG AATTCTTTCC AAATCCAACA AACAATGCAT CTCTCAATCC AGTTCTTGTC 
    AATGTAACCC AAGGCTGTGC TGGAGACAAC AGCTGCAAGT CCAACCCCTG TCACAATGGA 
    GGTGTTTGCC ATTCCCGGTG GGATGACTTC TCCTGTTCCT GTCCTGCCCT CACAAGTGGG 
    AAAGCCTGTG AGGAGGTTCA GTGGTGTGGA TTCAGCCCGT GTCCTCACGG AGCCCAGTGC 
    CAGCCGGTGC TTCAAGGATT TGAATGTATT GCAAATGCTG TTTTTAATGG ACAAAGCGGT 
    CAAATATTAT TCAGAAGCAA TGGGAATATT ACCAGAGAAC TCACCAATAT CACATTTGGT 
    TTCAGAACAA GGGATGCAAA TGTAATAATA TTGCATGCAG AAAAAGAGCC TGAATTTCTT 
    AATATTAGCA TTCAAGATTC CAGATTATTC TTTCAATTGC AAAGTGGCAA CAGCTTTTAT 
    ATGCTAAGTC TGACAAGTTT GCAGTCAGTG AATGATGGCA CATGGCACGA AGTGACCCTT 
    TCCATGACAG ACCCACTGTC CCAGACCTCC AGGTGGCAAA TGGAAGTGGA CAACGAAACA 
    CCTTTTGTGA CCAGCACAAT TGCTACTGGA AGCCTCAACT TTTTGAAGGA TAATACAGAT 
    ATTTATGTGG GAGACAGAGC TATTGACAAT ATAAAGGGCC TGCAAGGGTG TCTAAGTACA 
    ATAGAAATCG GAGGCATTTA TCTCTCTTAC TTTGAAAATG TTCATGGTTT CATTAATAAA 
    CCTCAGGAAG AGCAATTTCT CAAAATCTCT ACCAATTCAG TGGTCACTGG CTGTTTGCAG 
    TTAAATGTCT GCAACTCCAA CCCCTGTTTG CATGGAGGAA ACTGTGAAGA CATCTATAGC 
    TCTTATCATT GCTCCTGTCC CTTGGGATGG TCAGGGAAAC ACTGTGAACT CAACATCGAT 
    GAATGCTTTT CAAACCCCTG TATCCATGGC AACTGCTCTG ACAGAGTTGC AGCCTACCAC 
    TGCACATGTG AGCCTGGATA CACTGGTGTG AACTGTGAAG TGGATATAGA CAACTGCCAG 
    AGTCACCAGT GTGCAAATGG AGCCACCTGC ATTAGTCATA CTAATGGCTA TTCTTGCCTC 
    TGTTTTGGAA ATTTTACAGG AAAATTTTGC AGACAGAGCA GATTACCCTC AACAGTCTGT 
    GGGAATGAGA AGACAAATCT CACTTGCTAC AATGGAGGCA ACTGCACAGA GTTCCAGACT 
    GAATTAAAAT GTATGTGCCG GCCAGGTTTT ACTGGAGAAT GGTGTGAAAA GGACATTGAT 
    GAGTGTGCCT CTGATCCGTG TGTCAATGGA GGTCTGTGCC AGGACTTACT CAACAAATTC 
    CAGTGCCTCT GTGATGTTGC CTTTGCTGGC GAGCGCTGCG AGGTGGACTT GGCAGATGAC 
    TTGATCTCCG ACATTTTCAC CACTATTGGC TCAGTGACTG TCGCCTTGTT ACTGATCCTC 
    TTGCTGGCCA TTGTTGCTTC TGTTGTCACC TCCAACAAAA GGGCAACTCA GGGAACCTAC 
    AGCCCCAGCC GTCAGGAGAA GGAGGGCTCC CGAGTGGAAA TGTGGAACTT GATGCCACCC 
    CCTGCAATGG AGAGACTGAT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011507671.1
    CDS480..3143
    Translation

    Target ORF information:

    RefSeq Version XM_011509369.2
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011509369.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCTCTTC TACTTTTCCG AAGCAACAGG GATGTGTTTG TGAAGCTGGA GCTGCTAAGT 
    GGCTACATTC ACTTATCAAT TCAGGTCAAT AATCAGTCAA AGGTGCTTCT GTTCATTTCC 
    CACAACACCA GCGATGGAGA GTGGCATTTC GTGGAGGTAA TATTTGCAGA GGCTGTGACC 
    CTTACCTTAA TCGACGACTC CTGTAAGGAG AAATGCATCG CGAAAGCTCC TACTCCACTT 
    GAAAGTGATC AATCAATATG TGCTTTTCAG AACTCCTTTT TGGGTGGTTT ACCAGTGGGA 
    ATGACCAGCA ATGGTGTTGC TCTGCTTAAC TTCTATAATA TGCCATCCAC ACCTTCGTTT 
    GTAGGCTGTC TCCAAGACAT TAAAATTGAT TGGAATCACA TTACCCTGGA GAACATCTCG 
    TCTGGCTCAT CATTAAATGT CAAGGCAGGC TGTGTGAGAA AGGATTGGTG TGAAAGCCAA 
    CCTTGTCAAA GCAGAGGACG CTGCATCAAC TTGTGGCTGA GTTACCAGTG TGACTGCCAC 
    AGGCCCTATG AAGGCCCCAA CTGTCTGAGA GAGTATGTGG CAGGCAGATT TGGCCAGGAT 
    GACTCCACTG GTTATGTCAT CTTTACTCTT GATGAGAGCT ATGGAGACAC CATCAGCCTC 
    TCCATGTTTG TCCGAACGCT TCAACCATCA GGCTTACTTC TAGCTTTGGA AAACAGCACT 
    TATCAATATA TCCGTGTCTG GCTAGAGCGC GGCAGACTAG CAATGCTGAC TCCAAACTCT 
    CCCAAATTAG TAGTAAAATT TGTTCTTAAT GATGGAAATG TCCACTTGAT ATCTTTGAAA 
    ATCAAGCCAT ATAAAATTGA ACTGTATCAG TCTTCACAAA ACCTAGGATT TATTTCTGCT 
    TCTACGTGGA AAATCGAAAA GGGAGATGTC ATCTACATTG GTGGCCTACC TGACAAGCAA 
    GAGACTGAAC TTAATGGTGG ATTCTTCAAA GGCTGTATCC AAGATGTAAG ACTAAACAAC 
    CAAAATCTGG AATTCTTTCC AAATCCAACA AACAATGCAT CTCTCAATCC AGTTCTTGTC 
    AATGTAACCC AAGGCTGTGC TGGAGACAAC AGCTGCAAGT CCAACCCCTG TCACAATGGA 
    GGTGTTTGCC ATTCCCGGTG GGATGACTTC TCCTGTTCCT GTCCTGCCCT CACAAGTGGG 
    AAAGCCTGTG AGGAGGTTCA GTGGTGTGGA TTCAGCCCGT GTCCTCACGG AGCCCAGTGC 
    CAGCCGGTGC TTCAAGGATT TGAATGTATT GCAAATGCTG TTTTTAATGG ACAAAGCGGT 
    CAAATATTAT TCAGAAGCAA TGGGAATATT ACCAGAGAAC TCACCAATAT CACATTTGGT 
    TTCAGAACAA GGGATGCAAA TGTAATAATA TTGCATGCAG AAAAAGAGCC TGAATTTCTT 
    AATATTAGCA TTCAAGATTC CAGATTATTC TTTCAATTGC AAAGTGGCAA CAGCTTTTAT 
    ATGCTAAGTC TGACAAGTTT GCAGTCAGTG AATGATGGCA CATGGCACGA AGTGACCCTT 
    TCCATGACAG ACCCACTGTC CCAGACCTCC AGGTGGCAAA TGGAAGTGGA CAACGAAACA 
    CCTTTTGTGA CCAGCACAAT TGCTACTGGA AGCCTCAACT TTTTGAAGGA TAATACAGAT 
    ATTTATGTGG GAGACAGAGC TATTGACAAT ATAAAGGGCC TGCAAGGGTG TCTAAGTACA 
    ATAGAAATCG GAGGCATTTA TCTCTCTTAC TTTGAAAATG TTCATGGTTT CATTAATAAA 
    CCTCAGGAAG AGCAATTTCT CAAAATCTCT ACCAATTCAG TGGTCACTGG CTGTTTGCAG 
    TTAAATGTCT GCAACTCCAA CCCCTGTTTG CATGGAGGAA ACTGTGAAGA CATCTATAGC 
    TCTTATCATT GCTCCTGTCC CTTGGGATGG TCAGGGAAAC ACTGTGAACT CAACATCGAT 
    GAATGCTTTT CAAACCCCTG TATCCATGGC AACTGCTCTG ACAGAGTTGC AGCCTACCAC 
    TGCACATGTG AGCCTGGATA CACTGGTGTG AACTGTGAAG TGGATATAGA CAACTGCCAG 
    AGTCACCAGT GTGCAAATGG AGCCACCTGC ATTAGTCATA CTAATGGCTA TTCTTGCCTC 
    TGTTTTGGAA ATTTTACAGG AAAATTTTGC AGACAGAGCA GATTACCCTC AACAGTCTGT 
    GGGAATGAGA AGACAAATCT CACTTGCTAC AATGGAGGCA ACTGCACAGA GTTCCAGACT 
    GAATTAAAAT GTATGTGCCG GCCAGGTTTT ACTGGAGAAT GGTGTGAAAA GGACATTGAT 
    GAGTGTGCCT CTGATCCGTG TGTCAATGGA GGTCTGTGCC AGGACTTACT CAACAAATTC 
    CAGTGCCTCT GTGATGTTGC CTTTGCTGGC GAGCGCTGCG AGGTGGACTT GGCAGATGAC 
    TTGATCTCCG ACATTTTCAC CACTATTGGC TCAGTGACTG TCGCCTTGTT ACTGATCCTC 
    TTGCTGGCCA TTGTTGCTTC TGTTGTCACC TCCAACAAAA GGGCAACTCA GGGAACCTAC 
    AGCCCCAGCC GTCAGGAGAA GGAGGGCTCC CGAGTGGAAA TGTGGAACTT GATGCCACCC 
    CCTGCAATGG AGAGACTGAT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CRB1 ( NM_001257966.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244895.1 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 4, mRNA.
    Price & Availability↑
    CloneID OHu22808
    Clone ID Related Accession (Same CDS sequence) NM_001257966.1
    Accession Version NM_001257966.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2613bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC370545.1, AL513325.13, AK299368.1 and AY043325.1. Transcript Variant: This variant (4) lacks an alternate in-frame segment of two coding exons and most of a third compared to variant 1. The resulting isoform (4) has the same N- and C-termini but lacks an alternate internal segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299368.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA 
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC 
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA 
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT 
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA 
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT 
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG 
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC 
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA 
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC 
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG 
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA 
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA 
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC 
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT 
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC 
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG 
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT 
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA 
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT 
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT 
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC 
    CAAGATGGCC AGCATGGATT CAGCTGCCTG TGTCCATCTG GCTACACCGG GTCCCTGTGT 
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA 
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG 
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC 
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC 
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT 
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA 
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG 
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA 
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT 
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT 
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG 
    CCCTATGAAG GCCCCAACTG TCTGAGAGGA AAATTTTGCA GACAGAGCAG ATTACCCTCA 
    ACAGTCTGTG GGAATGAGAA GACAAATCTC ACTTGCTACA ATGGAGGCAA CTGCACAGAG 
    TTCCAGACTG AATTAAAATG TATGTGCCGG CCAGGTTTTA CTGGAGAATG GTGTGAAAAG 
    GACATTGATG AGTGTGCCTC TGATCCGTGT GTCAATGGAG GTCTGTGCCA GGACTTACTC 
    AACAAATTCC AGTGCCTCTG TGATGTTGCC TTTGCTGGCG AGCGCTGCGA GGTGGACTTG 
    GCAGATGACT TGATCTCCGA CATTTTCACC ACTATTGGCT CAGTGACTGT CGCCTTGTTA 
    CTGATCCTCT TGCTGGCCAT TGTTGCTTCT GTTGTCACCT CCAACAAAAG GGCAACTCAG 
    GGAACCTACA GCCCCAGCCG TCAGGAGAAG GAGGGCTCCC GAGTGGAAAT GTGGAACTTG 
    ATGCCACCCC CTGCAATGGA GAGACTGATT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001244895.1
    CDS210..2822
    Translation

    Target ORF information:

    RefSeq Version NM_001257966.1
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001257966.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA 
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC 
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA 
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT 
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA 
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT 
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG 
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC 
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA 
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC 
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG 
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA 
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA 
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC 
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT 
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC 
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG 
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT 
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA 
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT 
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT 
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC 
    CAAGATGGCC AGCATGGATT CAGCTGCCTG TGTCCATCTG GCTACACCGG GTCCCTGTGT 
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA 
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG 
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC 
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC 
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT 
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA 
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG 
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA 
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT 
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT 
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG 
    CCCTATGAAG GCCCCAACTG TCTGAGAGGA AAATTTTGCA GACAGAGCAG ATTACCCTCA 
    ACAGTCTGTG GGAATGAGAA GACAAATCTC ACTTGCTACA ATGGAGGCAA CTGCACAGAG 
    TTCCAGACTG AATTAAAATG TATGTGCCGG CCAGGTTTTA CTGGAGAATG GTGTGAAAAG 
    GACATTGATG AGTGTGCCTC TGATCCGTGT GTCAATGGAG GTCTGTGCCA GGACTTACTC 
    AACAAATTCC AGTGCCTCTG TGATGTTGCC TTTGCTGGCG AGCGCTGCGA GGTGGACTTG 
    GCAGATGACT TGATCTCCGA CATTTTCACC ACTATTGGCT CAGTGACTGT CGCCTTGTTA 
    CTGATCCTCT TGCTGGCCAT TGTTGCTTCT GTTGTCACCT CCAACAAAAG GGCAACTCAG 
    GGAACCTACA GCCCCAGCCG TCAGGAGAAG GAGGGCTCCC GAGTGGAAAT GTGGAACTTG 
    ATGCCACCCC CTGCAATGGA GAGACTGATT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CRB1 ( NM_201253.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_957705.1 Homo sapiens crumbs 1, cell polarity complex component (CRB1), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu19399
    Clone ID Related Accession (Same CDS sequence) NM_201253.2
    Accession Version NM_201253.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC370545.1, DC370777.1, BC136271.1 and AY043325.1. This sequence is a reference standard in the RefSeqGene project. On Aug 7, 2010 this sequence version replaced NM_201253.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY043325.1, SRR1803617.203976.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA 
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC 
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA 
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT 
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA 
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT 
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG 
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC 
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA 
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC 
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG 
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA 
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA 
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC 
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT 
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC 
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG 
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT 
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA 
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT 
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT 
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC 
    CAAGATGGCC AGCATGGATT CAGCTGCCTG TGTCCATCTG GCTACACCGG GTCCCTGTGT 
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA 
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG 
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC 
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC 
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT 
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA 
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG 
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA 
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT 
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT 
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG 
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC 
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC 
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT 
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC 
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC 
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT 
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG 
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA 
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT 
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT 
    GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA 
    GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG 
    CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA 
    ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC 
    AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT 
    ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG 
    CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC 
    ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT 
    TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT 
    TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA 
    GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT 
    CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA 
    AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT 
    TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA 
    TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC 
    ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT 
    CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT 
    TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG 
    AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA 
    TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG 
    TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG 
    TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACTTGGC AGATGACTTG 
    ATCTCCGACA TTTTCACCAC TATTGGCTCA GTGACTGTCG CCTTGTTACT GATCCTCTTG 
    CTGGCCATTG TTGCTTCTGT TGTCACCTCC AACAAAAGGG CAACTCAGGG AACCTACAGC 
    CCCAGCCGTC AGGAGAAGGA GGGCTCCCGA GTGGAAATGT GGAACTTGAT GCCACCCCCT 
    GCAATGGAGA GACTGATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_957705.1
    CDS210..4430
    Translation

    Target ORF information:

    RefSeq Version NM_201253.2
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs 1, cell polarity complex component (CRB1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_201253.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA 
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC 
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA 
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT 
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA 
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT 
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG 
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC 
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA 
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC 
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG 
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA 
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA 
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC 
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT 
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC 
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG 
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT 
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA 
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT 
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT 
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC 
    CAAGATGGCC AGCATGGATT CAGCTGCCTG TGTCCATCTG GCTACACCGG GTCCCTGTGT 
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA 
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG 
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC 
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC 
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT 
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA 
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG 
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA 
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT 
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT 
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG 
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC 
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC 
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT 
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC 
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC 
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT 
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG 
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA 
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT 
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT 
    GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA 
    GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG 
    CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA 
    ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC 
    AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT 
    ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG 
    CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC 
    ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT 
    TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT 
    TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA 
    GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT 
    CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA 
    AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT 
    TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA 
    TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC 
    ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT 
    CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT 
    TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG 
    AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA 
    TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG 
    TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG 
    TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACTTGGC AGATGACTTG 
    ATCTCCGACA TTTTCACCAC TATTGGCTCA GTGACTGTCG CCTTGTTACT GATCCTCTTG 
    CTGGCCATTG TTGCTTCTGT TGTCACCTCC AACAAAAGGG CAACTCAGGG AACCTACAGC 
    CCCAGCCGTC AGGAGAAGGA GGGCTCCCGA GTGGAAATGT GGAACTTGAT GCCACCCCCT 
    GCAATGGAGA GACTGATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CRB1 ( NM_201253.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_957705.1 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu107688
    Clone ID Related Accession (Same CDS sequence) NM_201253.3
    Accession Version NM_201253.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL513325.13, AL136322.24 and AL139136.17. On Nov 23, 2018 this sequence version replaced NM_201253.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY043325.1, SRR1803613.203862.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000367400.8/ ENSP00000356370.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA 
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC 
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA 
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT 
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA 
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT 
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG 
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC 
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA 
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC 
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG 
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA 
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA 
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC 
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT 
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC 
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG 
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT 
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA 
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT 
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT 
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC 
    CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT 
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA 
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG 
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC 
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC 
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT 
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA 
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG 
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA 
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT 
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT 
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG 
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC 
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC 
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT 
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC 
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC 
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT 
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG 
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA 
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT 
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT 
    GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA 
    GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG 
    CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA 
    ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC 
    AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT 
    ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG 
    CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC 
    ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT 
    TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT 
    TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA 
    GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT 
    CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA 
    AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT 
    TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA 
    TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC 
    ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT 
    CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT 
    TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG 
    AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA 
    TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG 
    TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG 
    TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACTTGGC AGATGACTTG 
    ATCTCCGACA TTTTCACCAC TATTGGCTCA GTGACTGTCG CCTTGTTACT GATCCTCTTG 
    CTGGCCATTG TTGCTTCTGT TGTCACCTCC AACAAAAGGG CAACTCAGGG AACCTACAGC 
    CCCAGCCGTC AGGAGAAGGA GGGCTCCCGA GTGGAAATGT GGAACTTGAT GCCACCCCCT 
    GCAATGGAGA GACTGATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_957705.1
    CDS162..4382
    Translation

    Target ORF information:

    RefSeq Version NM_201253.3
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_201253.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA 
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC 
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA 
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT 
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA 
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT 
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG 
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC 
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA 
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC 
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG 
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA 
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA 
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC 
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT 
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC 
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG 
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT 
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA 
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT 
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT 
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC 
    CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT 
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA 
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG 
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC 
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC 
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT 
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA 
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG 
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA 
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT 
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT 
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG 
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC 
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC 
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT 
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC 
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC 
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT 
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG 
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA 
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT 
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT 
    GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA 
    GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG 
    CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA 
    ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC 
    AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT 
    ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG 
    CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC 
    ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT 
    TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT 
    TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA 
    GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT 
    CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA 
    AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT 
    TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA 
    TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC 
    ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT 
    CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT 
    TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG 
    AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA 
    TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG 
    TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG 
    TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACTTGGC AGATGACTTG 
    ATCTCCGACA TTTTCACCAC TATTGGCTCA GTGACTGTCG CCTTGTTACT GATCCTCTTG 
    CTGGCCATTG TTGCTTCTGT TGTCACCTCC AACAAAAGGG CAACTCAGGG AACCTACAGC 
    CCCAGCCGTC AGGAGAAGGA GGGCTCCCGA GTGGAAATGT GGAACTTGAT GCCACCCCCT 
    GCAATGGAGA GACTGATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CRB1 ( NM_001193640.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001180569.1 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu108189
    Clone ID Related Accession (Same CDS sequence) NM_001193640.2
    Accession Version NM_001193640.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL513325.13, AL136322.24 and AL139136.17. On May 31, 2019 this sequence version replaced NM_001193640.1. Transcript Variant: This variant (2) lacks two in-frame exons compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.211656.1, SRR1803617.209649.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437, SAMN03465402 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA 
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC 
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA 
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT 
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA 
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT 
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG 
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC 
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA 
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGATACACA 
    GGTGCCCAGT GTGAGATCGA CCTCAATGAA TGCAATAGTA ACCCCTGCCA GTCCAATGGG 
    GAATGTGTGG AGCTGTCCTC AGAGAAACAA TATGGACGCA TCACTGGACT GCCTTCTTCT 
    TTCAGCTACC ATGAAGCCTC AGGTTATGTC TGTATCTGTC AGCCTGGATT CACAGGAATC 
    CACTGCGAAG AAGACGTCAA TGAATGTTCT TCAAACCCTT GCCAAAATGG TGGTACTTGT 
    GAGAACTTGC CTGGGAATTA TACTTGCCAT TGCCCATTTG ATAACCTTTC TAGAACTTTT 
    TATGGAGGAA GGGACTGTTC TGATATTCTC CTGGGCTGTA CCCATCAGCA ATGTCTAAAT 
    AATGGAACAT GCATCCCTCA CTTCCAAGAT GGCCAGCATG GATTCAGCTG CCTATGTCCA 
    TCTGGCTACA CCGGGTCCCT GTGTGAAATC GCAACCACAC TTTCATTTGA GGGCGATGGC 
    TTCCTGTGGG TCAAAAGTGG CTCAGTGACA ACCAAGGGCT CAGTTTGTAA CATAGCCCTC 
    AGGTTTCAGA CTGTTCAGCC AATGGCTCTT CTACTTTTCC GAAGCAACAG GGATGTGTTT 
    GTGAAGCTGG AGCTGCTAAG TGGCTACATT CACTTATCAA TTCAGGTCAA TAATCAGTCA 
    AAGGTGCTTC TGTTCATTTC CCACAACACC AGCGATGGAG AGTGGCATTT CGTGGAGGTA 
    ATATTTGCAG AGGCTGTGAC CCTTACCTTA ATCGACGACT CCTGTAAGGA GAAATGCATC 
    GCGAAAGCTC CTACTCCACT TGAAAGTGAT CAATCAATAT GTGCTTTTCA GAACTCCTTT 
    TTGGGTGGTT TACCAGTGGG AATGACCAGC AATGGTGTTG CTCTGCTTAA CTTCTATAAT 
    ATGCCATCCA CACCTTCGTT TGTAGGCTGT CTCCAAGACA TTAAAATTGA TTGGAATCAC 
    ATTACCCTGG AGAACATCTC GTCTGGCTCA TCATTAAATG TCAAGGCAGG CTGTGTGAGA 
    AAGGATTGGT GTGAAAGCCA ACCTTGTCAA AGCAGAGGAC GCTGCATCAA CTTGTGGCTG 
    AGTTACCAGT GTGACTGCCA CAGGCCCTAT GAAGGCCCCA ACTGTCTGAG AGAGTATGTG 
    GCAGGCAGAT TTGGCCAGGA TGACTCCACT GGTTATGTCA TCTTTACTCT TGATGAGAGC 
    TATGGAGACA CCATCAGCCT CTCCATGTTT GTCCGAACGC TTCAACCATC AGGCTTACTT 
    CTAGCTTTGG AAAACAGCAC TTATCAATAT ATCCGTGTCT GGCTAGAGCG CGGCAGACTA 
    GCAATGCTGA CTCCAAACTC TCCCAAATTA GTAGTAAAAT TTGTTCTTAA TGATGGAAAT 
    GTCCACTTGA TATCTTTGAA AATCAAGCCA TATAAAATTG AACTGTATCA GTCTTCACAA 
    AACCTAGGAT TTATTTCTGC TTCTACGTGG AAAATCGAAA AGGGAGATGT CATCTACATT 
    GGTGGCCTAC CTGACAAGCA AGAGACTGAA CTTAATGGTG GATTCTTCAA AGGCTGTATC 
    CAAGATGTAA GACTAAACAA CCAAAATCTG GAATTCTTTC CAAATCCAAC AAACAATGCA 
    TCTCTCAATC CAGTTCTTGT CAATGTAACC CAAGGCTGTG CTGGAGACAA CAGCTGCAAG 
    TCCAACCCCT GTCACAATGG AGGTGTTTGC CATTCCCGGT GGGATGACTT CTCCTGTTCC 
    TGTCCTGCCC TCACAAGTGG GAAAGCCTGT GAGGAGGTTC AGTGGTGTGG ATTCAGCCCG 
    TGTCCTCACG GAGCCCAGTG CCAGCCGGTG CTTCAAGGAT TTGAATGTAT TGCAAATGCT 
    GTTTTTAATG GACAAAGCGG TCAAATATTA TTCAGAAGCA ATGGGAATAT TACCAGAGAA 
    CTCACCAATA TCACATTTGG TTTCAGAACA AGGGATGCAA ATGTAATAAT ATTGCATGCA 
    GAAAAAGAGC CTGAATTTCT TAATATTAGC ATTCAAGATT CCAGATTATT CTTTCAATTG 
    CAAAGTGGCA ACAGCTTTTA TATGCTAAGT CTGACAAGTT TGCAGTCAGT GAATGATGGC 
    ACATGGCACG AAGTGACCCT TTCCATGACA GACCCACTGT CCCAGACCTC CAGGTGGCAA 
    ATGGAAGTGG ACAACGAAAC ACCTTTTGTG ACCAGCACAA TTGCTACTGG AAGCCTCAAC 
    TTTTTGAAGG ATAATACAGA TATTTATGTG GGAGACAGAG CTATTGACAA TATAAAGGGC 
    CTGCAAGGGT GTCTAAGTAC AATAGAAATC GGAGGCATTT ATCTCTCTTA CTTTGAAAAT 
    GTTCATGGTT TCATTAATAA ACCTCAGGAA GAGCAATTTC TCAAAATCTC TACCAATTCA 
    GTGGTCACTG GCTGTTTGCA GTTAAATGTC TGCAACTCCA ACCCCTGTTT GCATGGAGGA 
    AACTGTGAAG ACATCTATAG CTCTTATCAT TGCTCCTGTC CCTTGGGATG GTCAGGGAAA 
    CACTGTGAAC TCAACATCGA TGAATGCTTT TCAAACCCCT GTATCCATGG CAACTGCTCT 
    GACAGAGTTG CAGCCTACCA CTGCACATGT GAGCCTGGAT ACACTGGTGT GAACTGTGAA 
    GTGGATATAG ACAACTGCCA GAGTCACCAG TGTGCAAATG GAGCCACCTG CATTAGTCAT 
    ACTAATGGCT ATTCTTGCCT CTGTTTTGGA AATTTTACAG GAAAATTTTG CAGACAGAGC 
    AGATTACCCT CAACAGTCTG TGGGAATGAG AAGACAAATC TCACTTGCTA CAATGGAGGC 
    AACTGCACAG AGTTCCAGAC TGAATTAAAA TGTATGTGCC GGCCAGGTTT TACTGGAGAA 
    TGGTGTGAAA AGGACATTGA TGAGTGTGCC TCTGATCCGT GTGTCAATGG AGGTCTGTGC 
    CAGGACTTAC TCAACAAATT CCAGTGCCTC TGTGATGTTG CCTTTGCTGG CGAGCGCTGC 
    GAGGTGGACT TGGCAGATGA CTTGATCTCC GACATTTTCA CCACTATTGG CTCAGTGACT 
    GTCGCCTTGT TACTGATCCT CTTGCTGGCC ATTGTTGCTT CTGTTGTCAC CTCCAACAAA 
    AGGGCAACTC AGGGAACCTA CAGCCCCAGC CGTCAGGAGA AGGAGGGCTC CCGAGTGGAA 
    ATGTGGAACT TGATGCCACC CCCTGCAATG GAGAGACTGA TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180569.1
    CDS162..4046
    Translation

    Target ORF information:

    RefSeq Version NM_001193640.2
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001193640.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA 
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC 
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA 
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT 
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA 
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT 
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG 
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC 
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA 
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGATACACA 
    GGTGCCCAGT GTGAGATCGA CCTCAATGAA TGCAATAGTA ACCCCTGCCA GTCCAATGGG 
    GAATGTGTGG AGCTGTCCTC AGAGAAACAA TATGGACGCA TCACTGGACT GCCTTCTTCT 
    TTCAGCTACC ATGAAGCCTC AGGTTATGTC TGTATCTGTC AGCCTGGATT CACAGGAATC 
    CACTGCGAAG AAGACGTCAA TGAATGTTCT TCAAACCCTT GCCAAAATGG TGGTACTTGT 
    GAGAACTTGC CTGGGAATTA TACTTGCCAT TGCCCATTTG ATAACCTTTC TAGAACTTTT 
    TATGGAGGAA GGGACTGTTC TGATATTCTC CTGGGCTGTA CCCATCAGCA ATGTCTAAAT 
    AATGGAACAT GCATCCCTCA CTTCCAAGAT GGCCAGCATG GATTCAGCTG CCTATGTCCA 
    TCTGGCTACA CCGGGTCCCT GTGTGAAATC GCAACCACAC TTTCATTTGA GGGCGATGGC 
    TTCCTGTGGG TCAAAAGTGG CTCAGTGACA ACCAAGGGCT CAGTTTGTAA CATAGCCCTC 
    AGGTTTCAGA CTGTTCAGCC AATGGCTCTT CTACTTTTCC GAAGCAACAG GGATGTGTTT 
    GTGAAGCTGG AGCTGCTAAG TGGCTACATT CACTTATCAA TTCAGGTCAA TAATCAGTCA 
    AAGGTGCTTC TGTTCATTTC CCACAACACC AGCGATGGAG AGTGGCATTT CGTGGAGGTA 
    ATATTTGCAG AGGCTGTGAC CCTTACCTTA ATCGACGACT CCTGTAAGGA GAAATGCATC 
    GCGAAAGCTC CTACTCCACT TGAAAGTGAT CAATCAATAT GTGCTTTTCA GAACTCCTTT 
    TTGGGTGGTT TACCAGTGGG AATGACCAGC AATGGTGTTG CTCTGCTTAA CTTCTATAAT 
    ATGCCATCCA CACCTTCGTT TGTAGGCTGT CTCCAAGACA TTAAAATTGA TTGGAATCAC 
    ATTACCCTGG AGAACATCTC GTCTGGCTCA TCATTAAATG TCAAGGCAGG CTGTGTGAGA 
    AAGGATTGGT GTGAAAGCCA ACCTTGTCAA AGCAGAGGAC GCTGCATCAA CTTGTGGCTG 
    AGTTACCAGT GTGACTGCCA CAGGCCCTAT GAAGGCCCCA ACTGTCTGAG AGAGTATGTG 
    GCAGGCAGAT TTGGCCAGGA TGACTCCACT GGTTATGTCA TCTTTACTCT TGATGAGAGC 
    TATGGAGACA CCATCAGCCT CTCCATGTTT GTCCGAACGC TTCAACCATC AGGCTTACTT 
    CTAGCTTTGG AAAACAGCAC TTATCAATAT ATCCGTGTCT GGCTAGAGCG CGGCAGACTA 
    GCAATGCTGA CTCCAAACTC TCCCAAATTA GTAGTAAAAT TTGTTCTTAA TGATGGAAAT 
    GTCCACTTGA TATCTTTGAA AATCAAGCCA TATAAAATTG AACTGTATCA GTCTTCACAA 
    AACCTAGGAT TTATTTCTGC TTCTACGTGG AAAATCGAAA AGGGAGATGT CATCTACATT 
    GGTGGCCTAC CTGACAAGCA AGAGACTGAA CTTAATGGTG GATTCTTCAA AGGCTGTATC 
    CAAGATGTAA GACTAAACAA CCAAAATCTG GAATTCTTTC CAAATCCAAC AAACAATGCA 
    TCTCTCAATC CAGTTCTTGT CAATGTAACC CAAGGCTGTG CTGGAGACAA CAGCTGCAAG 
    TCCAACCCCT GTCACAATGG AGGTGTTTGC CATTCCCGGT GGGATGACTT CTCCTGTTCC 
    TGTCCTGCCC TCACAAGTGG GAAAGCCTGT GAGGAGGTTC AGTGGTGTGG ATTCAGCCCG 
    TGTCCTCACG GAGCCCAGTG CCAGCCGGTG CTTCAAGGAT TTGAATGTAT TGCAAATGCT 
    GTTTTTAATG GACAAAGCGG TCAAATATTA TTCAGAAGCA ATGGGAATAT TACCAGAGAA 
    CTCACCAATA TCACATTTGG TTTCAGAACA AGGGATGCAA ATGTAATAAT ATTGCATGCA 
    GAAAAAGAGC CTGAATTTCT TAATATTAGC ATTCAAGATT CCAGATTATT CTTTCAATTG 
    CAAAGTGGCA ACAGCTTTTA TATGCTAAGT CTGACAAGTT TGCAGTCAGT GAATGATGGC 
    ACATGGCACG AAGTGACCCT TTCCATGACA GACCCACTGT CCCAGACCTC CAGGTGGCAA 
    ATGGAAGTGG ACAACGAAAC ACCTTTTGTG ACCAGCACAA TTGCTACTGG AAGCCTCAAC 
    TTTTTGAAGG ATAATACAGA TATTTATGTG GGAGACAGAG CTATTGACAA TATAAAGGGC 
    CTGCAAGGGT GTCTAAGTAC AATAGAAATC GGAGGCATTT ATCTCTCTTA CTTTGAAAAT 
    GTTCATGGTT TCATTAATAA ACCTCAGGAA GAGCAATTTC TCAAAATCTC TACCAATTCA 
    GTGGTCACTG GCTGTTTGCA GTTAAATGTC TGCAACTCCA ACCCCTGTTT GCATGGAGGA 
    AACTGTGAAG ACATCTATAG CTCTTATCAT TGCTCCTGTC CCTTGGGATG GTCAGGGAAA 
    CACTGTGAAC TCAACATCGA TGAATGCTTT TCAAACCCCT GTATCCATGG CAACTGCTCT 
    GACAGAGTTG CAGCCTACCA CTGCACATGT GAGCCTGGAT ACACTGGTGT GAACTGTGAA 
    GTGGATATAG ACAACTGCCA GAGTCACCAG TGTGCAAATG GAGCCACCTG CATTAGTCAT 
    ACTAATGGCT ATTCTTGCCT CTGTTTTGGA AATTTTACAG GAAAATTTTG CAGACAGAGC 
    AGATTACCCT CAACAGTCTG TGGGAATGAG AAGACAAATC TCACTTGCTA CAATGGAGGC 
    AACTGCACAG AGTTCCAGAC TGAATTAAAA TGTATGTGCC GGCCAGGTTT TACTGGAGAA 
    TGGTGTGAAA AGGACATTGA TGAGTGTGCC TCTGATCCGT GTGTCAATGG AGGTCTGTGC 
    CAGGACTTAC TCAACAAATT CCAGTGCCTC TGTGATGTTG CCTTTGCTGG CGAGCGCTGC 
    GAGGTGGACT TGGCAGATGA CTTGATCTCC GACATTTTCA CCACTATTGG CTCAGTGACT 
    GTCGCCTTGT TACTGATCCT CTTGCTGGCC ATTGTTGCTT CTGTTGTCAC CTCCAACAAA 
    AGGGCAACTC AGGGAACCTA CAGCCCCAGC CGTCAGGAGA AGGAGGGCTC CCGAGTGGAA 
    ATGTGGAACT TGATGCCACC CCCTGCAATG GAGAGACTGA TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CRB1 ( NM_001257965.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244894.1 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID OHu108193
    Clone ID Related Accession (Same CDS sequence) NM_001257965.2
    Accession Version NM_001257965.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356315.12, AL513325.13, AL136322.24 and AL139136.17. On May 31, 2019 this sequence version replaced NM_001257965.1. Transcript Variant: This variant (3) contains three noncoding exons in place of the first exon and contains an alternate in-frame exon compared to variant 1. The resulting isoform (3) is shorter at the N- terminus and contains an alternate internal segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302826.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968832, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGACC CTTGCTTCTC CAATCCCTGT CAAGGAAGTG CCACTTGTGT GAACACCCCA 
    GGAGAAAGGA GCTTTCTGTG CAAATGTCCT CCTGGGTACA GTGGGACAAT CTGTGAAACT 
    ACCATTGGTT CCTGTGGCAA GAACTCCTGC CAACATGGAG GTATTTGCCA TCAGGACCCT 
    ATTTATCCTG TCTGCATCTG CCCTGCTGGA TATGCTGGAA GATTCTGTGA GATAGATCAC 
    GATGAGTGTG CTTCCAGCCC TTGCCAAAAT GGGGCCGTGT GCCAGGATGG AATTGATGGT 
    TACTCCTGCT TCTGTGTCCC AGGATATCAA GGCAGACACT GCGACTTGGA AGTGGATGAA 
    TGTGCTTCAG ATCCCTGCAA GAACGAGGCT ACATGCCTCA ATGAAATAGG AAGATATACT 
    TGTATCTGTC CCCACAATTA TTCTGGTGTA AACTGTGAAT TGGAAATTGA CGAATGTTGG 
    TCCCAGCCTT GTTTAAATGG TGCAACTTGT CAGGATGCTC TGGGGGCCTA TTTCTGCGAC 
    TGTGCCCCTG GATTCCTGGG GGATCACTGT GAACTCAACA CTGATGAGTG TGCCAGTCAA 
    CCTTGTCTCC ATGGAGGGCT GTGTGTGGAT GGAGAAAACA GATATAGCTG TAACTGCACG 
    GGTAGTGGAT TCACAGGGAC ACACTGTGAG ACCTTGATGC CTCTTTGTTG GTCAAAACCT 
    TGTCACAATA ATGCTACATG TGAGGACAGT GTTGACAATT ACACTTGTCA CTGCTGGCCT 
    GGATACACAG GTGCCCAGTG TGAGATCGAC CTCAATGAAT GCAATAGTAA CCCCTGCCAG 
    TCCAATGGGG AATGTGTGGA GCTGTCCTCA GAGAAACAAT ATGGACGCAT CACTGGACTG 
    CCTTCTTCTT TCAGCTACCA TGAAGCCTCA GGTTATGTCT GTATCTGTCA GCCTGGATTC 
    ACAGGAATCC ACTGCGAAGA AGACGTCAAT GAATGTTCTT CAAACCCTTG CCAAAATGGT 
    GGTACTTGTG AGAACTTGCC TGGGAATTAT ACTTGCCATT GCCCATTTGA TAACCTTTCT 
    AGAACTTTTT ATGGAGGAAG GGACTGTTCT GATATTCTCC TGGGCTGTAC CCATCAGCAA 
    TGTCTAAATA ATGGAACATG CATCCCTCAC TTCCAAGATG GCCAGCATGG ATTCAGCTGC 
    CTATGTCCAT CTGGCTACAC CGGGTCCCTG TGTGAAATCG CAACCACACT TTCATTTGAG 
    GGCGATGGCT TCCTGTGGGT CAAAAGTGGC TCAGTGACAA CCAAGGGCTC AGTTTGTAAC 
    ATAGCCCTCA GGTTTCAGAC TGTTCAGCCA ATGGCTCTTC TACTTTTCCG AAGCAACAGG 
    GATGTGTTTG TGAAGCTGGA GCTGCTAAGT GGCTACATTC ACTTATCAAT TCAGGTCAAT 
    AATCAGTCAA AGGTGCTTCT GTTCATTTCC CACAACACCA GCGATGGAGA GTGGCATTTC 
    GTGGAGGTAA TATTTGCAGA GGCTGTGACC CTTACCTTAA TCGACGACTC CTGTAAGGAG 
    AAATGCATCG CGAAAGCTCC TACTCCACTT GAAAGTGATC AATCAATATG TGCTTTTCAG 
    AACTCCTTTT TGGGTGGTTT ACCAGTGGGA ATGACCAGCA ATGGTGTTGC TCTGCTTAAC 
    TTCTATAATA TGCCATCCAC ACCTTCGTTT GTAGGCTGTC TCCAAGACAT TAAAATTGAT 
    TGGAATCACA TTACCCTGGA GAACATCTCG TCTGGCTCAT CATTAAATGT CAAGGCAGGC 
    TGTGTGAGAA AGGATTGGTG TGAAAGCCAA CCTTGTCAAA GCAGAGGACG CTGCATCAAC 
    TTGTGGCTGA GTTACCAGTG TGACTGCCAC AGGCCCTATG AAGGCCCCAA CTGTCTGAGA 
    GAGTATGTGG CAGGCAGATT TGGCCAGGAT GACTCCACTG GTTATGTCAT CTTTACTCTT 
    GATGAGAGCT ATGGAGACAC CATCAGCCTC TCCATGTTTG TCCGAACGCT TCAACCATCA 
    GGCTTACTTC TAGCTTTGGA AAACAGCACT TATCAATATA TCCGTGTCTG GCTAGAGCGC 
    GGCAGACTAG CAATGCTGAC TCCAAACTCT CCCAAATTAG TAGTAAAATT TGTTCTTAAT 
    GATGGAAATG TCCACTTGAT ATCTTTGAAA ATCAAGCCAT ATAAAATTGA ACTGTATCAG 
    TCTTCACAAA ACCTAGGATT TATTTCTGCT TCTACGTGGA AAATCGAAAA GGGAGATGTC 
    ATCTACATTG GTGGCCTACC TGACAAGCAA GAGACTGAAC TTAATGGTGG ATTCTTCAAA 
    GGCTGTATCC AAGATGTAAG ACTAAACAAC CAAAATCTGG AATTCTTTCC AAATCCAACA 
    AACAATGCAT CTCTCAATCC AGTTCTTGTC AATGTAACCC AAGGCTGTGC TGGAGACAAC 
    AGCTGCAAGA GGCAGACCAA TGTGGGAAGG GCACTCACTG AGTTGGGATC CAGAGGACCT 
    AAGTACCAAG TTTCACTGTT TCGCTTCTGT GTAGGATCTT GGGCAACTGG AAACACCTTC 
    TTTTTATCAT CTATAAAACC AGGATCCAAC CCCTGTCACA ATGGAGGTGT TTGCCATTCC 
    CGGTGGGATG ACTTCTCCTG TTCCTGTCCT GCCCTCACAA GTGGGAAAGC CTGTGAGGAG 
    GTTCAGTGGT GTGGATTCAG CCCGTGTCCT CACGGAGCCC AGTGCCAGCC GGTGCTTCAA 
    GGATTTGAAT GTATTGCAAA TGCTGTTTTT AATGGACAAA GCGGTCAAAT ATTATTCAGA 
    AGCAATGGGA ATATTACCAG AGAACTCACC AATATCACAT TTGGTTTCAG AACAAGGGAT 
    GCAAATGTAA TAATATTGCA TGCAGAAAAA GAGCCTGAAT TTCTTAATAT TAGCATTCAA 
    GATTCCAGAT TATTCTTTCA ATTGCAAAGT GGCAACAGCT TTTATATGCT AAGTCTGACA 
    AGTTTGCAGT CAGTGAATGA TGGCACATGG CACGAAGTGA CCCTTTCCAT GACAGACCCA 
    CTGTCCCAGA CCTCCAGGTG GCAAATGGAA GTGGACAACG AAACACCTTT TGTGACCAGC 
    ACAATTGCTA CTGGAAGCCT CAACTTTTTG AAGGATAATA CAGATATTTA TGTGGGAGAC 
    AGAGCTATTG ACAATATAAA GGGCCTGCAA GGGTGTCTAA GTACAATAGA AATCGGAGGC 
    ATTTATCTCT CTTACTTTGA AAATGTTCAT GGTTTCATTA ATAAACCTCA GGAAGAGCAA 
    TTTCTCAAAA TCTCTACCAA TTCAGTGGTC ACTGGCTGTT TGCAGTTAAA TGTCTGCAAC 
    TCCAACCCCT GTTTGCATGG AGGAAACTGT GAAGACATCT ATAGCTCTTA TCATTGCTCC 
    TGTCCCTTGG GATGGTCAGG GAAACACTGT GAACTCAACA TCGATGAATG CTTTTCAAAC 
    CCCTGTATCC ATGGCAACTG CTCTGACAGA GTTGCAGCCT ACCACTGCAC ATGTGAGCCT 
    GGATACACTG GTGTGAACTG TGAAGTGGAT ATAGACAACT GCCAGAGTCA CCAGTGTGCA 
    AATGGAGCCA CCTGCATTAG TCATACTAAT GGCTATTCTT GCCTCTGTTT TGGAAATTTT 
    ACAGGAAAAT TTTGCAGACA GAGCAGATTA CCCTCAACAG TCTGTGGGAA TGAGAAGACA 
    AATCTCACTT GCTACAATGG AGGCAACTGC ACAGAGTTCC AGACTGAATT AAAATGTATG 
    TGCCGGCCAG GTTTTACTGG AGAATGGTGT GAAAAGGACA TTGATGAGTG TGCCTCTGAT 
    CCGTGTGTCA ATGGAGGTCT GTGCCAGGAC TTACTCAACA AATTCCAGTG CCTCTGTGAT 
    GTTGCCTTTG CTGGCGAGCG CTGCGAGGTG GACTTGGCAG ATGACTTGAT CTCCGACATT 
    TTCACCACTA TTGGCTCAGT GACTGTCGCC TTGTTACTGA TCCTCTTGCT GGCCATTGTT 
    GCTTCTGTTG TCACCTCCAA CAAAAGGGCA ACTCAGGGAA CCTACAGCCC CAGCCGTCAG 
    GAGAAGGAGG GCTCCCGAGT GGAAATGTGG AACTTGATGC CACCCCCTGC AATGGAGAGA 
    CTGATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001244894.1
    CDS298..4446
    Translation

    Target ORF information:

    RefSeq Version NM_001257965.2
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001257965.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGACC CTTGCTTCTC CAATCCCTGT CAAGGAAGTG CCACTTGTGT GAACACCCCA 
    GGAGAAAGGA GCTTTCTGTG CAAATGTCCT CCTGGGTACA GTGGGACAAT CTGTGAAACT 
    ACCATTGGTT CCTGTGGCAA GAACTCCTGC CAACATGGAG GTATTTGCCA TCAGGACCCT 
    ATTTATCCTG TCTGCATCTG CCCTGCTGGA TATGCTGGAA GATTCTGTGA GATAGATCAC 
    GATGAGTGTG CTTCCAGCCC TTGCCAAAAT GGGGCCGTGT GCCAGGATGG AATTGATGGT 
    TACTCCTGCT TCTGTGTCCC AGGATATCAA GGCAGACACT GCGACTTGGA AGTGGATGAA 
    TGTGCTTCAG ATCCCTGCAA GAACGAGGCT ACATGCCTCA ATGAAATAGG AAGATATACT 
    TGTATCTGTC CCCACAATTA TTCTGGTGTA AACTGTGAAT TGGAAATTGA CGAATGTTGG 
    TCCCAGCCTT GTTTAAATGG TGCAACTTGT CAGGATGCTC TGGGGGCCTA TTTCTGCGAC 
    TGTGCCCCTG GATTCCTGGG GGATCACTGT GAACTCAACA CTGATGAGTG TGCCAGTCAA 
    CCTTGTCTCC ATGGAGGGCT GTGTGTGGAT GGAGAAAACA GATATAGCTG TAACTGCACG 
    GGTAGTGGAT TCACAGGGAC ACACTGTGAG ACCTTGATGC CTCTTTGTTG GTCAAAACCT 
    TGTCACAATA ATGCTACATG TGAGGACAGT GTTGACAATT ACACTTGTCA CTGCTGGCCT 
    GGATACACAG GTGCCCAGTG TGAGATCGAC CTCAATGAAT GCAATAGTAA CCCCTGCCAG 
    TCCAATGGGG AATGTGTGGA GCTGTCCTCA GAGAAACAAT ATGGACGCAT CACTGGACTG 
    CCTTCTTCTT TCAGCTACCA TGAAGCCTCA GGTTATGTCT GTATCTGTCA GCCTGGATTC 
    ACAGGAATCC ACTGCGAAGA AGACGTCAAT GAATGTTCTT CAAACCCTTG CCAAAATGGT 
    GGTACTTGTG AGAACTTGCC TGGGAATTAT ACTTGCCATT GCCCATTTGA TAACCTTTCT 
    AGAACTTTTT ATGGAGGAAG GGACTGTTCT GATATTCTCC TGGGCTGTAC CCATCAGCAA 
    TGTCTAAATA ATGGAACATG CATCCCTCAC TTCCAAGATG GCCAGCATGG ATTCAGCTGC 
    CTATGTCCAT CTGGCTACAC CGGGTCCCTG TGTGAAATCG CAACCACACT TTCATTTGAG 
    GGCGATGGCT TCCTGTGGGT CAAAAGTGGC TCAGTGACAA CCAAGGGCTC AGTTTGTAAC 
    ATAGCCCTCA GGTTTCAGAC TGTTCAGCCA ATGGCTCTTC TACTTTTCCG AAGCAACAGG 
    GATGTGTTTG TGAAGCTGGA GCTGCTAAGT GGCTACATTC ACTTATCAAT TCAGGTCAAT 
    AATCAGTCAA AGGTGCTTCT GTTCATTTCC CACAACACCA GCGATGGAGA GTGGCATTTC 
    GTGGAGGTAA TATTTGCAGA GGCTGTGACC CTTACCTTAA TCGACGACTC CTGTAAGGAG 
    AAATGCATCG CGAAAGCTCC TACTCCACTT GAAAGTGATC AATCAATATG TGCTTTTCAG 
    AACTCCTTTT TGGGTGGTTT ACCAGTGGGA ATGACCAGCA ATGGTGTTGC TCTGCTTAAC 
    TTCTATAATA TGCCATCCAC ACCTTCGTTT GTAGGCTGTC TCCAAGACAT TAAAATTGAT 
    TGGAATCACA TTACCCTGGA GAACATCTCG TCTGGCTCAT CATTAAATGT CAAGGCAGGC 
    TGTGTGAGAA AGGATTGGTG TGAAAGCCAA CCTTGTCAAA GCAGAGGACG CTGCATCAAC 
    TTGTGGCTGA GTTACCAGTG TGACTGCCAC AGGCCCTATG AAGGCCCCAA CTGTCTGAGA 
    GAGTATGTGG CAGGCAGATT TGGCCAGGAT GACTCCACTG GTTATGTCAT CTTTACTCTT 
    GATGAGAGCT ATGGAGACAC CATCAGCCTC TCCATGTTTG TCCGAACGCT TCAACCATCA 
    GGCTTACTTC TAGCTTTGGA AAACAGCACT TATCAATATA TCCGTGTCTG GCTAGAGCGC 
    GGCAGACTAG CAATGCTGAC TCCAAACTCT CCCAAATTAG TAGTAAAATT TGTTCTTAAT 
    GATGGAAATG TCCACTTGAT ATCTTTGAAA ATCAAGCCAT ATAAAATTGA ACTGTATCAG 
    TCTTCACAAA ACCTAGGATT TATTTCTGCT TCTACGTGGA AAATCGAAAA GGGAGATGTC 
    ATCTACATTG GTGGCCTACC TGACAAGCAA GAGACTGAAC TTAATGGTGG ATTCTTCAAA 
    GGCTGTATCC AAGATGTAAG ACTAAACAAC CAAAATCTGG AATTCTTTCC AAATCCAACA 
    AACAATGCAT CTCTCAATCC AGTTCTTGTC AATGTAACCC AAGGCTGTGC TGGAGACAAC 
    AGCTGCAAGA GGCAGACCAA TGTGGGAAGG GCACTCACTG AGTTGGGATC CAGAGGACCT 
    AAGTACCAAG TTTCACTGTT TCGCTTCTGT GTAGGATCTT GGGCAACTGG AAACACCTTC 
    TTTTTATCAT CTATAAAACC AGGATCCAAC CCCTGTCACA ATGGAGGTGT TTGCCATTCC 
    CGGTGGGATG ACTTCTCCTG TTCCTGTCCT GCCCTCACAA GTGGGAAAGC CTGTGAGGAG 
    GTTCAGTGGT GTGGATTCAG CCCGTGTCCT CACGGAGCCC AGTGCCAGCC GGTGCTTCAA 
    GGATTTGAAT GTATTGCAAA TGCTGTTTTT AATGGACAAA GCGGTCAAAT ATTATTCAGA 
    AGCAATGGGA ATATTACCAG AGAACTCACC AATATCACAT TTGGTTTCAG AACAAGGGAT 
    GCAAATGTAA TAATATTGCA TGCAGAAAAA GAGCCTGAAT TTCTTAATAT TAGCATTCAA 
    GATTCCAGAT TATTCTTTCA ATTGCAAAGT GGCAACAGCT TTTATATGCT AAGTCTGACA 
    AGTTTGCAGT CAGTGAATGA TGGCACATGG CACGAAGTGA CCCTTTCCAT GACAGACCCA 
    CTGTCCCAGA CCTCCAGGTG GCAAATGGAA GTGGACAACG AAACACCTTT TGTGACCAGC 
    ACAATTGCTA CTGGAAGCCT CAACTTTTTG AAGGATAATA CAGATATTTA TGTGGGAGAC 
    AGAGCTATTG ACAATATAAA GGGCCTGCAA GGGTGTCTAA GTACAATAGA AATCGGAGGC 
    ATTTATCTCT CTTACTTTGA AAATGTTCAT GGTTTCATTA ATAAACCTCA GGAAGAGCAA 
    TTTCTCAAAA TCTCTACCAA TTCAGTGGTC ACTGGCTGTT TGCAGTTAAA TGTCTGCAAC 
    TCCAACCCCT GTTTGCATGG AGGAAACTGT GAAGACATCT ATAGCTCTTA TCATTGCTCC 
    TGTCCCTTGG GATGGTCAGG GAAACACTGT GAACTCAACA TCGATGAATG CTTTTCAAAC 
    CCCTGTATCC ATGGCAACTG CTCTGACAGA GTTGCAGCCT ACCACTGCAC ATGTGAGCCT 
    GGATACACTG GTGTGAACTG TGAAGTGGAT ATAGACAACT GCCAGAGTCA CCAGTGTGCA 
    AATGGAGCCA CCTGCATTAG TCATACTAAT GGCTATTCTT GCCTCTGTTT TGGAAATTTT 
    ACAGGAAAAT TTTGCAGACA GAGCAGATTA CCCTCAACAG TCTGTGGGAA TGAGAAGACA 
    AATCTCACTT GCTACAATGG AGGCAACTGC ACAGAGTTCC AGACTGAATT AAAATGTATG 
    TGCCGGCCAG GTTTTACTGG AGAATGGTGT GAAAAGGACA TTGATGAGTG TGCCTCTGAT 
    CCGTGTGTCA ATGGAGGTCT GTGCCAGGAC TTACTCAACA AATTCCAGTG CCTCTGTGAT 
    GTTGCCTTTG CTGGCGAGCG CTGCGAGGTG GACTTGGCAG ATGACTTGAT CTCCGACATT 
    TTCACCACTA TTGGCTCAGT GACTGTCGCC TTGTTACTGA TCCTCTTGCT GGCCATTGTT 
    GCTTCTGTTG TCACCTCCAA CAAAAGGGCA ACTCAGGGAA CCTACAGCCC CAGCCGTCAG 
    GAGAAGGAGG GCTCCCGAGT GGAAATGTGG AACTTGATGC CACCCCCTGC AATGGAGAGA 
    CTGATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CRB1 ( XM_017000851.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856340.1 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OHu87140
    Clone ID Related Accession (Same CDS sequence) XM_017000851.1
    Accession Version XM_017000851.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3378bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTGCAAC TTGTCAGGAT GCTCTGGGGG CCTATTTCTG CGACTGTGCC CCTGGATTCC 
    TGGGGGATCA CTGTGAACTC AACACTGATG AGTGTGCCAG TCAACCTTGT CTCCATGGAG 
    GGCTGTGTGT GGATGGAGAA AACAGGATAC ACAGGTGCCC AGTGTGAGAT CGACCTCAAT 
    GAATGCAATA GTAACCCCTG CCAGTCCAAT GGGGAATGTG TGGAGCTGTC CTCAGAGAAA 
    CAATATGGAC GCATCACTGG ACTGCCTTCT TCTTTCAGCT ACCATGAAGC CTCAGGTTAT 
    GTCTGTATCT GTCAGCCTGG ATTCACAGGA ATCCACTGCG AAGAAGACGT CAATGAATGT 
    TCTTCAAACC CTTGCCAAAA TGGTGGTACT TGTGAGAACT TGCCTGGGAA TTATACTTGC 
    CATTGCCCAT TTGATAACCT TTCTAGAACT TTTTATGGAG GAAGGGACTG TTCTGATATT 
    CTCCTGGGCT GTACCCATCA GCAATGTCTA AATAATGGAA CATGCATCCC TCACTTCCAA 
    GATGGCCAGC ATGGATTCAG CTGCCTATGT CCATCTGGCT ACACCGGGTC CCTGTGTGAA 
    ATCGCAACCA CACTTTCATT TGAGGGCGAT GGCTTCCTGT GGGTCAAAAG TGGCTCAGTG 
    ACAACCAAGG GCTCAGTTTG TAACATAGCC CTCAGGTTTC AGACTGTTCA GCCAATGGCT 
    CTTCTACTTT TCCGAAGCAA CAGGGATGTG TTTGTGAAGC TGGAGCTGCT AAGTGGCTAC 
    ATTCACTTAT CAATTCAGGT CAATAATCAG TCAAAGGTGC TTCTGTTCAT TTCCCACAAC 
    ACCAGCGATG GAGAGTGGCA TTTCGTGGAG GTAATATTTG CAGAGGCTGT GACCCTTACC 
    TTAATCGACG ACTCCTGTAA GGAGAAATGC ATCGCGAAAG CTCCTACTCC ACTTGAAAGT 
    GATCAATCAA TATGTGCTTT TCAGAACTCC TTTTTGGGTG GTTTACCAGT GGGAATGACC 
    AGCAATGGTG TTGCTCTGCT TAACTTCTAT AATATGCCAT CCACACCTTC GTTTGTAGGC 
    TGTCTCCAAG ACATTAAAAT TGATTGGAAT CACATTACCC TGGAGAACAT CTCGTCTGGC 
    TCATCATTAA ATGTCAAGGC AGGCTGTGTG AGAAAGGATT GGTGTGAAAG CCAACCTTGT 
    CAAAGCAGAG GACGCTGCAT CAACTTGTGG CTGAGTTACC AGTGTGACTG CCACAGGCCC 
    TATGAAGGCC CCAACTGTCT GAGAGAGTAT GTGGCAGGCA GATTTGGCCA GGATGACTCC 
    ACTGGTTATG TCATCTTTAC TCTTGATGAG AGCTATGGAG ACACCATCAG CCTCTCCATG 
    TTTGTCCGAA CGCTTCAACC ATCAGGCTTA CTTCTAGCTT TGGAAAACAG CACTTATCAA 
    TATATCCGTG TCTGGCTAGA GCGCGGCAGA CTAGCAATGC TGACTCCAAA CTCTCCCAAA 
    TTAGTAGTAA AATTTGTTCT TAATGATGGA AATGTCCACT TGATATCTTT GAAAATCAAG 
    CCATATAAAA TTGAACTGTA TCAGTCTTCA CAAAACCTAG GATTTATTTC TGCTTCTACG 
    TGGAAAATCG AAAAGGGAGA TGTCATCTAC ATTGGTGGCC TACCTGACAA GCAAGAGACT 
    GAACTTAATG GTGGATTCTT CAAAGGCTGT ATCCAAGATG TAAGACTAAA CAACCAAAAT 
    CTGGAATTCT TTCCAAATCC AACAAACAAT GCATCTCTCA ATCCAGTTCT TGTCAATGTA 
    ACCCAAGGCT GTGCTGGAGA CAACAGCTGC AAGTCCAACC CCTGTCACAA TGGAGGTGTT 
    TGCCATTCCC GGTGGGATGA CTTCTCCTGT TCCTGTCCTG CCCTCACAAG TGGGAAAGCC 
    TGTGAGGAGG TTCAGTGGTG TGGATTCAGC CCGTGTCCTC ACGGAGCCCA GTGCCAGCCG 
    GTGCTTCAAG GATTTGAATG TATTGCAAAT GCTGTTTTTA ATGGACAAAG CGGTCAAATA 
    TTATTCAGAA GCAATGGGAA TATTACCAGA GAACTCACCA ATATCACATT TGGTTTCAGA 
    ACAAGGGATG CAAATGTAAT AATATTGCAT GCAGAAAAAG AGCCTGAATT TCTTAATATT 
    AGCATTCAAG ATTCCAGATT ATTCTTTCAA TTGCAAAGTG GCAACAGCTT TTATATGCTA 
    AGTCTGACAA GTTTGCAGTC AGTGAATGAT GGCACATGGC ACGAAGTGAC CCTTTCCATG 
    ACAGACCCAC TGTCCCAGAC CTCCAGGTGG CAAATGGAAG TGGACAACGA AACACCTTTT 
    GTGACCAGCA CAATTGCTAC TGGAAGCCTC AACTTTTTGA AGGATAATAC AGATATTTAT 
    GTGGGAGACA GAGCTATTGA CAATATAAAG GGCCTGCAAG GGTGTCTAAG TACAATAGAA 
    ATCGGAGGCA TTTATCTCTC TTACTTTGAA AATGTTCATG GTTTCATTAA TAAACCTCAG 
    GAAGAGCAAT TTCTCAAAAT CTCTACCAAT TCAGTGGTCA CTGGCTGTTT GCAGTTAAAT 
    GTCTGCAACT CCAACCCCTG TTTGCATGGA GGAAACTGTG AAGACATCTA TAGCTCTTAT 
    CATTGCTCCT GTCCCTTGGG ATGGTCAGGG AAACACTGTG AACTCAACAT CGATGAATGC 
    TTTTCAAACC CCTGTATCCA TGGCAACTGC TCTGACAGAG TTGCAGCCTA CCACTGCACA 
    TGTGAGCCTG GATACACTGG TGTGAACTGT GAAGTGGATA TAGACAACTG CCAGAGTCAC 
    CAGTGTGCAA ATGGAGCCAC CTGCATTAGT CATACTAATG GCTATTCTTG CCTCTGTTTT 
    GGAAATTTTA CAGGAAAATT TTGCAGACAG AGCAGATTAC CCTCAACAGT CTGTGGGAAT 
    GAGAAGACAA ATCTCACTTG CTACAATGGA GGCAACTGCA CAGAGTTCCA GACTGAATTA 
    AAATGTATGT GCCGGCCAGG TTTTACTGGA GAATGGTGTG AAAAGGACAT TGATGAGTGT 
    GCCTCTGATC CGTGTGTCAA TGGAGGTCTG TGCCAGGACT TACTCAACAA ATTCCAGTGC 
    CTCTGTGATG TTGCCTTTGC TGGCGAGCGC TGCGAGGTGG ACTTGGCAGA TGACTTGATC 
    TCCGACATTT TCACCACTAT TGGCTCAGTG ACTGTCGCCT TGTTACTGAT CCTCTTGCTG 
    GCCATTGTTG CTTCTGTTGT CACCTCCAAC AAAAGGGCAA CTCAGGGAAC CTACAGCCCC 
    AGCCGTCAGG AGAAGGAGGG CTCCCGAGTG GAAATGTGGA ACTTGATGCC ACCCCCTGCA 
    ATGGAGAGAC TGATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016856340.1
    CDS745..4122
    Translation

    Target ORF information:

    RefSeq Version XM_017000851.1
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017000851.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTGCAAC TTGTCAGGAT GCTCTGGGGG CCTATTTCTG CGACTGTGCC CCTGGATTCC 
    TGGGGGATCA CTGTGAACTC AACACTGATG AGTGTGCCAG TCAACCTTGT CTCCATGGAG 
    GGCTGTGTGT GGATGGAGAA AACAGGATAC ACAGGTGCCC AGTGTGAGAT CGACCTCAAT 
    GAATGCAATA GTAACCCCTG CCAGTCCAAT GGGGAATGTG TGGAGCTGTC CTCAGAGAAA 
    CAATATGGAC GCATCACTGG ACTGCCTTCT TCTTTCAGCT ACCATGAAGC CTCAGGTTAT 
    GTCTGTATCT GTCAGCCTGG ATTCACAGGA ATCCACTGCG AAGAAGACGT CAATGAATGT 
    TCTTCAAACC CTTGCCAAAA TGGTGGTACT TGTGAGAACT TGCCTGGGAA TTATACTTGC 
    CATTGCCCAT TTGATAACCT TTCTAGAACT TTTTATGGAG GAAGGGACTG TTCTGATATT 
    CTCCTGGGCT GTACCCATCA GCAATGTCTA AATAATGGAA CATGCATCCC TCACTTCCAA 
    GATGGCCAGC ATGGATTCAG CTGCCTATGT CCATCTGGCT ACACCGGGTC CCTGTGTGAA 
    ATCGCAACCA CACTTTCATT TGAGGGCGAT GGCTTCCTGT GGGTCAAAAG TGGCTCAGTG 
    ACAACCAAGG GCTCAGTTTG TAACATAGCC CTCAGGTTTC AGACTGTTCA GCCAATGGCT 
    CTTCTACTTT TCCGAAGCAA CAGGGATGTG TTTGTGAAGC TGGAGCTGCT AAGTGGCTAC 
    ATTCACTTAT CAATTCAGGT CAATAATCAG TCAAAGGTGC TTCTGTTCAT TTCCCACAAC 
    ACCAGCGATG GAGAGTGGCA TTTCGTGGAG GTAATATTTG CAGAGGCTGT GACCCTTACC 
    TTAATCGACG ACTCCTGTAA GGAGAAATGC ATCGCGAAAG CTCCTACTCC ACTTGAAAGT 
    GATCAATCAA TATGTGCTTT TCAGAACTCC TTTTTGGGTG GTTTACCAGT GGGAATGACC 
    AGCAATGGTG TTGCTCTGCT TAACTTCTAT AATATGCCAT CCACACCTTC GTTTGTAGGC 
    TGTCTCCAAG ACATTAAAAT TGATTGGAAT CACATTACCC TGGAGAACAT CTCGTCTGGC 
    TCATCATTAA ATGTCAAGGC AGGCTGTGTG AGAAAGGATT GGTGTGAAAG CCAACCTTGT 
    CAAAGCAGAG GACGCTGCAT CAACTTGTGG CTGAGTTACC AGTGTGACTG CCACAGGCCC 
    TATGAAGGCC CCAACTGTCT GAGAGAGTAT GTGGCAGGCA GATTTGGCCA GGATGACTCC 
    ACTGGTTATG TCATCTTTAC TCTTGATGAG AGCTATGGAG ACACCATCAG CCTCTCCATG 
    TTTGTCCGAA CGCTTCAACC ATCAGGCTTA CTTCTAGCTT TGGAAAACAG CACTTATCAA 
    TATATCCGTG TCTGGCTAGA GCGCGGCAGA CTAGCAATGC TGACTCCAAA CTCTCCCAAA 
    TTAGTAGTAA AATTTGTTCT TAATGATGGA AATGTCCACT TGATATCTTT GAAAATCAAG 
    CCATATAAAA TTGAACTGTA TCAGTCTTCA CAAAACCTAG GATTTATTTC TGCTTCTACG 
    TGGAAAATCG AAAAGGGAGA TGTCATCTAC ATTGGTGGCC TACCTGACAA GCAAGAGACT 
    GAACTTAATG GTGGATTCTT CAAAGGCTGT ATCCAAGATG TAAGACTAAA CAACCAAAAT 
    CTGGAATTCT TTCCAAATCC AACAAACAAT GCATCTCTCA ATCCAGTTCT TGTCAATGTA 
    ACCCAAGGCT GTGCTGGAGA CAACAGCTGC AAGTCCAACC CCTGTCACAA TGGAGGTGTT 
    TGCCATTCCC GGTGGGATGA CTTCTCCTGT TCCTGTCCTG CCCTCACAAG TGGGAAAGCC 
    TGTGAGGAGG TTCAGTGGTG TGGATTCAGC CCGTGTCCTC ACGGAGCCCA GTGCCAGCCG 
    GTGCTTCAAG GATTTGAATG TATTGCAAAT GCTGTTTTTA ATGGACAAAG CGGTCAAATA 
    TTATTCAGAA GCAATGGGAA TATTACCAGA GAACTCACCA ATATCACATT TGGTTTCAGA 
    ACAAGGGATG CAAATGTAAT AATATTGCAT GCAGAAAAAG AGCCTGAATT TCTTAATATT 
    AGCATTCAAG ATTCCAGATT ATTCTTTCAA TTGCAAAGTG GCAACAGCTT TTATATGCTA 
    AGTCTGACAA GTTTGCAGTC AGTGAATGAT GGCACATGGC ACGAAGTGAC CCTTTCCATG 
    ACAGACCCAC TGTCCCAGAC CTCCAGGTGG CAAATGGAAG TGGACAACGA AACACCTTTT 
    GTGACCAGCA CAATTGCTAC TGGAAGCCTC AACTTTTTGA AGGATAATAC AGATATTTAT 
    GTGGGAGACA GAGCTATTGA CAATATAAAG GGCCTGCAAG GGTGTCTAAG TACAATAGAA 
    ATCGGAGGCA TTTATCTCTC TTACTTTGAA AATGTTCATG GTTTCATTAA TAAACCTCAG 
    GAAGAGCAAT TTCTCAAAAT CTCTACCAAT TCAGTGGTCA CTGGCTGTTT GCAGTTAAAT 
    GTCTGCAACT CCAACCCCTG TTTGCATGGA GGAAACTGTG AAGACATCTA TAGCTCTTAT 
    CATTGCTCCT GTCCCTTGGG ATGGTCAGGG AAACACTGTG AACTCAACAT CGATGAATGC 
    TTTTCAAACC CCTGTATCCA TGGCAACTGC TCTGACAGAG TTGCAGCCTA CCACTGCACA 
    TGTGAGCCTG GATACACTGG TGTGAACTGT GAAGTGGATA TAGACAACTG CCAGAGTCAC 
    CAGTGTGCAA ATGGAGCCAC CTGCATTAGT CATACTAATG GCTATTCTTG CCTCTGTTTT 
    GGAAATTTTA CAGGAAAATT TTGCAGACAG AGCAGATTAC CCTCAACAGT CTGTGGGAAT 
    GAGAAGACAA ATCTCACTTG CTACAATGGA GGCAACTGCA CAGAGTTCCA GACTGAATTA 
    AAATGTATGT GCCGGCCAGG TTTTACTGGA GAATGGTGTG AAAAGGACAT TGATGAGTGT 
    GCCTCTGATC CGTGTGTCAA TGGAGGTCTG TGCCAGGACT TACTCAACAA ATTCCAGTGC 
    CTCTGTGATG TTGCCTTTGC TGGCGAGCGC TGCGAGGTGG ACTTGGCAGA TGACTTGATC 
    TCCGACATTT TCACCACTAT TGGCTCAGTG ACTGTCGCCT TGTTACTGAT CCTCTTGCTG 
    GCCATTGTTG CTTCTGTTGT CACCTCCAAC AAAAGGGCAA CTCAGGGAAC CTACAGCCCC 
    AGCCGTCAGG AGAAGGAGGG CTCCCGAGTG GAAATGTGGA ACTTGATGCC ACCCCCTGCA 
    ATGGAGAGAC TGATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CRB1 ( XM_017000852.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856341.1 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OHu87141
    Clone ID Related Accession (Same CDS sequence) XM_017000852.1
    Accession Version XM_017000852.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA 
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC 
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA 
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT 
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA 
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT 
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG 
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC 
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA 
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC 
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG 
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA 
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA 
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC 
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT 
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC 
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG 
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT 
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA 
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT 
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT 
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC 
    CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT 
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA 
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG 
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC 
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC 
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT 
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA 
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG 
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA 
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT 
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT 
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG 
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC 
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC 
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT 
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC 
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC 
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT 
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG 
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA 
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT 
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGAGGC AGACCAATGT GGGAAGGGCA 
    CTCACTGAGT TGGGATCCAG AGGACCTAAG TACCAAGTTT CACTGTTTCG CTTCTGTGTA 
    GGATCTTGGG CAACTGGAAA CACCTTCTTT TTATCATCTA TAAAACCAGG ATCCAACCCC 
    TGTCACAATG GAGGTGTTTG CCATTCCCGG TGGGATGACT TCTCCTGTTC CTGTCCTGCC 
    CTCACAAGTG GGAAAGCCTG TGAGGAGGTT CAGTGGTGTG GATTCAGCCC GTGTCCTCAC 
    GGAGCCCAGT GCCAGCCGGT GCTTCAAGGA TTTGAATGTA TTGCAAATGC TGTTTTTAAT 
    GGACAAAGCG GTCAAATATT ATTCAGAAGC AATGGGAATA TTACCAGAGA ACTCACCAAT 
    ATCACATTTG GTTTCAGAAC AAGGGATGCA AATGTAATAA TATTGCATGC AGAAAAAGAG 
    CCTGAATTTC TTAATATTAG CATTCAAGAT TCCAGATTAT TCTTTCAATT GCAAAGTGGC 
    AACAGCTTTT ATATGCTAAG TCTGACAAGT TTGCAGTCAG TGAATGATGG CACATGGCAC 
    GAAGTGACCC TTTCCATGAC AGACCCACTG TCCCAGACCT CCAGGTGGCA AATGGAAGTG 
    GACAACGAAA CACCTTTTGT GACCAGCACA ATTGCTACTG GAAGCCTCAA CTTTTTGAAG 
    GATAATACAG ATATTTATGT GGGAGACAGA GCTATTGACA ATATAAAGGG CCTGCAAGGG 
    TGTCTAAGTA CAATAGAAAT CGGAGGCATT TATCTCTCTT ACTTTGAAAA TGTTCATGGT 
    TTCATTAATA AACCTCAGGA AGAGCAATTT CTCAAAATCT CTACCAATTC AGTGGTCACT 
    GGCTGTTTGC AGTTAAATGT CTGCAACTCC AACCCCTGTT TGCATGGAGG AAACTGTGAA 
    GACATCTATA GCTCTTATCA TTGCTCCTGT CCCTTGGGAT GGTCAGGGAA ACACTGTGAA 
    CTCAACATCG ATGAATGCTT TTCAAACCCC TGTATCCATG GCAACTGCTC TGACAGAGTT 
    GCAGCCTACC ACTGCACATG TGAGCCTGGA TACACTGGTG TGAACTGTGA AGTGGATATA 
    GACAACTGCC AGAGTCACCA GTGTGCAAAT GGAGCCACCT GCATTAGTCA TACTAATGGC 
    TATTCTTGCC TCTGTTTTGG AAATTTTACA GGAAAATTTT GCAGACAGAG CAGATTACCC 
    TCAACAGTCT GTGGGAATGA GAAGACAAAT CTCACTTGCT ACAATGGAGG CAACTGCACA 
    GAGTTCCAGA CTGAATTAAA ATGTATGTGC CGGCCAGGTT TTACTGGAGA ATGGTGTGAA 
    AAGGACATTG ATGAGTGTGC CTCTGATCCG TGTGTCAATG GAGGTCTGTG CCAGGACTTA 
    CTCAACAAAT TCCAGTGCCT CTGTGATGTT GCCTTTGCTG GCGAGCGCTG CGAGGTGGAC 
    TTGGCAGATG ACTTGATCTC CGACATTTTC ACCACTATTG GCTCAGTGAC TGTCGCCTTG 
    TTACTGATCC TCTTGCTGGC CATTGTTGCT TCTGTTGTCA CCTCCAACAA AAGGGCAACT 
    CAGGGAACCT ACAGCCCCAG CCGTCAGGAG AAGGAGGGCT CCCGAGTGGA AATGTGGAAC 
    TTGATGCCAC CCCCTGCAAT GGAGAGACTG ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016856341.1
    CDS135..4490
    Translation

    Target ORF information:

    RefSeq Version XM_017000852.1
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017000852.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA 
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC 
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA 
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT 
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA 
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT 
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG 
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC 
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA 
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC 
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG 
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA 
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA 
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC 
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT 
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC 
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG 
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT 
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA 
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT 
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT 
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC 
    CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT 
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA 
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG 
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC 
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC 
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT 
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA 
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG 
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA 
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT 
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT 
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG 
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC 
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC 
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT 
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC 
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC 
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT 
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG 
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA 
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT 
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGAGGC AGACCAATGT GGGAAGGGCA 
    CTCACTGAGT TGGGATCCAG AGGACCTAAG TACCAAGTTT CACTGTTTCG CTTCTGTGTA 
    GGATCTTGGG CAACTGGAAA CACCTTCTTT TTATCATCTA TAAAACCAGG ATCCAACCCC 
    TGTCACAATG GAGGTGTTTG CCATTCCCGG TGGGATGACT TCTCCTGTTC CTGTCCTGCC 
    CTCACAAGTG GGAAAGCCTG TGAGGAGGTT CAGTGGTGTG GATTCAGCCC GTGTCCTCAC 
    GGAGCCCAGT GCCAGCCGGT GCTTCAAGGA TTTGAATGTA TTGCAAATGC TGTTTTTAAT 
    GGACAAAGCG GTCAAATATT ATTCAGAAGC AATGGGAATA TTACCAGAGA ACTCACCAAT 
    ATCACATTTG GTTTCAGAAC AAGGGATGCA AATGTAATAA TATTGCATGC AGAAAAAGAG 
    CCTGAATTTC TTAATATTAG CATTCAAGAT TCCAGATTAT TCTTTCAATT GCAAAGTGGC 
    AACAGCTTTT ATATGCTAAG TCTGACAAGT TTGCAGTCAG TGAATGATGG CACATGGCAC 
    GAAGTGACCC TTTCCATGAC AGACCCACTG TCCCAGACCT CCAGGTGGCA AATGGAAGTG 
    GACAACGAAA CACCTTTTGT GACCAGCACA ATTGCTACTG GAAGCCTCAA CTTTTTGAAG 
    GATAATACAG ATATTTATGT GGGAGACAGA GCTATTGACA ATATAAAGGG CCTGCAAGGG 
    TGTCTAAGTA CAATAGAAAT CGGAGGCATT TATCTCTCTT ACTTTGAAAA TGTTCATGGT 
    TTCATTAATA AACCTCAGGA AGAGCAATTT CTCAAAATCT CTACCAATTC AGTGGTCACT 
    GGCTGTTTGC AGTTAAATGT CTGCAACTCC AACCCCTGTT TGCATGGAGG AAACTGTGAA 
    GACATCTATA GCTCTTATCA TTGCTCCTGT CCCTTGGGAT GGTCAGGGAA ACACTGTGAA 
    CTCAACATCG ATGAATGCTT TTCAAACCCC TGTATCCATG GCAACTGCTC TGACAGAGTT 
    GCAGCCTACC ACTGCACATG TGAGCCTGGA TACACTGGTG TGAACTGTGA AGTGGATATA 
    GACAACTGCC AGAGTCACCA GTGTGCAAAT GGAGCCACCT GCATTAGTCA TACTAATGGC 
    TATTCTTGCC TCTGTTTTGG AAATTTTACA GGAAAATTTT GCAGACAGAG CAGATTACCC 
    TCAACAGTCT GTGGGAATGA GAAGACAAAT CTCACTTGCT ACAATGGAGG CAACTGCACA 
    GAGTTCCAGA CTGAATTAAA ATGTATGTGC CGGCCAGGTT TTACTGGAGA ATGGTGTGAA 
    AAGGACATTG ATGAGTGTGC CTCTGATCCG TGTGTCAATG GAGGTCTGTG CCAGGACTTA 
    CTCAACAAAT TCCAGTGCCT CTGTGATGTT GCCTTTGCTG GCGAGCGCTG CGAGGTGGAC 
    TTGGCAGATG ACTTGATCTC CGACATTTTC ACCACTATTG GCTCAGTGAC TGTCGCCTTG 
    TTACTGATCC TCTTGCTGGC CATTGTTGCT TCTGTTGTCA CCTCCAACAA AAGGGCAACT 
    CAGGGAACCT ACAGCCCCAG CCGTCAGGAG AAGGAGGGCT CCCGAGTGGA AATGTGGAAC 
    TTGATGCCAC CCCCTGCAAT GGAGAGACTG ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CRB1 ( NM_001193640.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001180569.1 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu17475
    Clone ID Related Accession (Same CDS sequence) NM_001193640.1
    Accession Version NM_001193640.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC370545.1, DC370777.1, AJ748821.1 and AY043325.1. Transcript Variant: This variant (2) lacks two in-frame exons compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.211656.1, SRR1803617.209649.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437, SAMN03465402 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA 
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC 
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA 
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT 
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA 
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT 
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG 
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC 
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA 
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGATACACA 
    GGTGCCCAGT GTGAGATCGA CCTCAATGAA TGCAATAGTA ACCCCTGCCA GTCCAATGGG 
    GAATGTGTGG AGCTGTCCTC AGAGAAACAA TATGGACGCA TCACTGGACT GCCTTCTTCT 
    TTCAGCTACC ATGAAGCCTC AGGTTATGTC TGTATCTGTC AGCCTGGATT CACAGGAATC 
    CACTGCGAAG AAGACGTCAA TGAATGTTCT TCAAACCCTT GCCAAAATGG TGGTACTTGT 
    GAGAACTTGC CTGGGAATTA TACTTGCCAT TGCCCATTTG ATAACCTTTC TAGAACTTTT 
    TATGGAGGAA GGGACTGTTC TGATATTCTC CTGGGCTGTA CCCATCAGCA ATGTCTAAAT 
    AATGGAACAT GCATCCCTCA CTTCCAAGAT GGCCAGCATG GATTCAGCTG CCTGTGTCCA 
    TCTGGCTACA CCGGGTCCCT GTGTGAAATC GCAACCACAC TTTCATTTGA GGGCGATGGC 
    TTCCTGTGGG TCAAAAGTGG CTCAGTGACA ACCAAGGGCT CAGTTTGTAA CATAGCCCTC 
    AGGTTTCAGA CTGTTCAGCC AATGGCTCTT CTACTTTTCC GAAGCAACAG GGATGTGTTT 
    GTGAAGCTGG AGCTGCTAAG TGGCTACATT CACTTATCAA TTCAGGTCAA TAATCAGTCA 
    AAGGTGCTTC TGTTCATTTC CCACAACACC AGCGATGGAG AGTGGCATTT CGTGGAGGTA 
    ATATTTGCAG AGGCTGTGAC CCTTACCTTA ATCGACGACT CCTGTAAGGA GAAATGCATC 
    GCGAAAGCTC CTACTCCACT TGAAAGTGAT CAATCAATAT GTGCTTTTCA GAACTCCTTT 
    TTGGGTGGTT TACCAGTGGG AATGACCAGC AATGGTGTTG CTCTGCTTAA CTTCTATAAT 
    ATGCCATCCA CACCTTCGTT TGTAGGCTGT CTCCAAGACA TTAAAATTGA TTGGAATCAC 
    ATTACCCTGG AGAACATCTC GTCTGGCTCA TCATTAAATG TCAAGGCAGG CTGTGTGAGA 
    AAGGATTGGT GTGAAAGCCA ACCTTGTCAA AGCAGAGGAC GCTGCATCAA CTTGTGGCTG 
    AGTTACCAGT GTGACTGCCA CAGGCCCTAT GAAGGCCCCA ACTGTCTGAG AGAGTATGTG 
    GCAGGCAGAT TTGGCCAGGA TGACTCCACT GGTTATGTCA TCTTTACTCT TGATGAGAGC 
    TATGGAGACA CCATCAGCCT CTCCATGTTT GTCCGAACGC TTCAACCATC AGGCTTACTT 
    CTAGCTTTGG AAAACAGCAC TTATCAATAT ATCCGTGTCT GGCTAGAGCG CGGCAGACTA 
    GCAATGCTGA CTCCAAACTC TCCCAAATTA GTAGTAAAAT TTGTTCTTAA TGATGGAAAT 
    GTCCACTTGA TATCTTTGAA AATCAAGCCA TATAAAATTG AACTGTATCA GTCTTCACAA 
    AACCTAGGAT TTATTTCTGC TTCTACGTGG AAAATCGAAA AGGGAGATGT CATCTACATT 
    GGTGGCCTAC CTGACAAGCA AGAGACTGAA CTTAATGGTG GATTCTTCAA AGGCTGTATC 
    CAAGATGTAA GACTAAACAA CCAAAATCTG GAATTCTTTC CAAATCCAAC AAACAATGCA 
    TCTCTCAATC CAGTTCTTGT CAATGTAACC CAAGGCTGTG CTGGAGACAA CAGCTGCAAG 
    TCCAACCCCT GTCACAATGG AGGTGTTTGC CATTCCCGGT GGGATGACTT CTCCTGTTCC 
    TGTCCTGCCC TCACAAGTGG GAAAGCCTGT GAGGAGGTTC AGTGGTGTGG ATTCAGCCCG 
    TGTCCTCACG GAGCCCAGTG CCAGCCGGTG CTTCAAGGAT TTGAATGTAT TGCAAATGCT 
    GTTTTTAATG GACAAAGCGG TCAAATATTA TTCAGAAGCA ATGGGAATAT TACCAGAGAA 
    CTCACCAATA TCACATTTGG TTTCAGAACA AGGGATGCAA ATGTAATAAT ATTGCATGCA 
    GAAAAAGAGC CTGAATTTCT TAATATTAGC ATTCAAGATT CCAGATTATT CTTTCAATTG 
    CAAAGTGGCA ACAGCTTTTA TATGCTAAGT CTGACAAGTT TGCAGTCAGT GAATGATGGC 
    ACATGGCACG AAGTGACCCT TTCCATGACA GACCCACTGT CCCAGACCTC CAGGTGGCAA 
    ATGGAAGTGG ACAACGAAAC ACCTTTTGTG ACCAGCACAA TTGCTACTGG AAGCCTCAAC 
    TTTTTGAAGG ATAATACAGA TATTTATGTG GGAGACAGAG CTATTGACAA TATAAAGGGC 
    CTGCAAGGGT GTCTAAGTAC AATAGAAATC GGAGGCATTT ATCTCTCTTA CTTTGAAAAT 
    GTTCATGGTT TCATTAATAA ACCTCAGGAA GAGCAATTTC TCAAAATCTC TACCAATTCA 
    GTGGTCACTG GCTGTTTGCA GTTAAATGTC TGCAACTCCA ACCCCTGTTT GCATGGAGGA 
    AACTGTGAAG ACATCTATAG CTCTTATCAT TGCTCCTGTC CCTTGGGATG GTCAGGGAAA 
    CACTGTGAAC TCAACATCGA TGAATGCTTT TCAAACCCCT GTATCCATGG CAACTGCTCT 
    GACAGAGTTG CAGCCTACCA CTGCACATGT GAGCCTGGAT ACACTGGTGT GAACTGTGAA 
    GTGGATATAG ACAACTGCCA GAGTCACCAG TGTGCAAATG GAGCCACCTG CATTAGTCAT 
    ACTAATGGCT ATTCTTGCCT CTGTTTTGGA AATTTTACAG GAAAATTTTG CAGACAGAGC 
    AGATTACCCT CAACAGTCTG TGGGAATGAG AAGACAAATC TCACTTGCTA CAATGGAGGC 
    AACTGCACAG AGTTCCAGAC TGAATTAAAA TGTATGTGCC GGCCAGGTTT TACTGGAGAA 
    TGGTGTGAAA AGGACATTGA TGAGTGTGCC TCTGATCCGT GTGTCAATGG AGGTCTGTGC 
    CAGGACTTAC TCAACAAATT CCAGTGCCTC TGTGATGTTG CCTTTGCTGG CGAGCGCTGC 
    GAGGTGGACT TGGCAGATGA CTTGATCTCC GACATTTTCA CCACTATTGG CTCAGTGACT 
    GTCGCCTTGT TACTGATCCT CTTGCTGGCC ATTGTTGCTT CTGTTGTCAC CTCCAACAAA 
    AGGGCAACTC AGGGAACCTA CAGCCCCAGC CGTCAGGAGA AGGAGGGCTC CCGAGTGGAA 
    ATGTGGAACT TGATGCCACC CCCTGCAATG GAGAGACTGA TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180569.1
    CDS210..4094
    Translation

    Target ORF information:

    RefSeq Version NM_001193640.1
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001193640.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA 
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC 
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA 
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT 
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA 
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT 
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG 
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC 
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA 
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGATACACA 
    GGTGCCCAGT GTGAGATCGA CCTCAATGAA TGCAATAGTA ACCCCTGCCA GTCCAATGGG 
    GAATGTGTGG AGCTGTCCTC AGAGAAACAA TATGGACGCA TCACTGGACT GCCTTCTTCT 
    TTCAGCTACC ATGAAGCCTC AGGTTATGTC TGTATCTGTC AGCCTGGATT CACAGGAATC 
    CACTGCGAAG AAGACGTCAA TGAATGTTCT TCAAACCCTT GCCAAAATGG TGGTACTTGT 
    GAGAACTTGC CTGGGAATTA TACTTGCCAT TGCCCATTTG ATAACCTTTC TAGAACTTTT 
    TATGGAGGAA GGGACTGTTC TGATATTCTC CTGGGCTGTA CCCATCAGCA ATGTCTAAAT 
    AATGGAACAT GCATCCCTCA CTTCCAAGAT GGCCAGCATG GATTCAGCTG CCTGTGTCCA 
    TCTGGCTACA CCGGGTCCCT GTGTGAAATC GCAACCACAC TTTCATTTGA GGGCGATGGC 
    TTCCTGTGGG TCAAAAGTGG CTCAGTGACA ACCAAGGGCT CAGTTTGTAA CATAGCCCTC 
    AGGTTTCAGA CTGTTCAGCC AATGGCTCTT CTACTTTTCC GAAGCAACAG GGATGTGTTT 
    GTGAAGCTGG AGCTGCTAAG TGGCTACATT CACTTATCAA TTCAGGTCAA TAATCAGTCA 
    AAGGTGCTTC TGTTCATTTC CCACAACACC AGCGATGGAG AGTGGCATTT CGTGGAGGTA 
    ATATTTGCAG AGGCTGTGAC CCTTACCTTA ATCGACGACT CCTGTAAGGA GAAATGCATC 
    GCGAAAGCTC CTACTCCACT TGAAAGTGAT CAATCAATAT GTGCTTTTCA GAACTCCTTT 
    TTGGGTGGTT TACCAGTGGG AATGACCAGC AATGGTGTTG CTCTGCTTAA CTTCTATAAT 
    ATGCCATCCA CACCTTCGTT TGTAGGCTGT CTCCAAGACA TTAAAATTGA TTGGAATCAC 
    ATTACCCTGG AGAACATCTC GTCTGGCTCA TCATTAAATG TCAAGGCAGG CTGTGTGAGA 
    AAGGATTGGT GTGAAAGCCA ACCTTGTCAA AGCAGAGGAC GCTGCATCAA CTTGTGGCTG 
    AGTTACCAGT GTGACTGCCA CAGGCCCTAT GAAGGCCCCA ACTGTCTGAG AGAGTATGTG 
    GCAGGCAGAT TTGGCCAGGA TGACTCCACT GGTTATGTCA TCTTTACTCT TGATGAGAGC 
    TATGGAGACA CCATCAGCCT CTCCATGTTT GTCCGAACGC TTCAACCATC AGGCTTACTT 
    CTAGCTTTGG AAAACAGCAC TTATCAATAT ATCCGTGTCT GGCTAGAGCG CGGCAGACTA 
    GCAATGCTGA CTCCAAACTC TCCCAAATTA GTAGTAAAAT TTGTTCTTAA TGATGGAAAT 
    GTCCACTTGA TATCTTTGAA AATCAAGCCA TATAAAATTG AACTGTATCA GTCTTCACAA 
    AACCTAGGAT TTATTTCTGC TTCTACGTGG AAAATCGAAA AGGGAGATGT CATCTACATT 
    GGTGGCCTAC CTGACAAGCA AGAGACTGAA CTTAATGGTG GATTCTTCAA AGGCTGTATC 
    CAAGATGTAA GACTAAACAA CCAAAATCTG GAATTCTTTC CAAATCCAAC AAACAATGCA 
    TCTCTCAATC CAGTTCTTGT CAATGTAACC CAAGGCTGTG CTGGAGACAA CAGCTGCAAG 
    TCCAACCCCT GTCACAATGG AGGTGTTTGC CATTCCCGGT GGGATGACTT CTCCTGTTCC 
    TGTCCTGCCC TCACAAGTGG GAAAGCCTGT GAGGAGGTTC AGTGGTGTGG ATTCAGCCCG 
    TGTCCTCACG GAGCCCAGTG CCAGCCGGTG CTTCAAGGAT TTGAATGTAT TGCAAATGCT 
    GTTTTTAATG GACAAAGCGG TCAAATATTA TTCAGAAGCA ATGGGAATAT TACCAGAGAA 
    CTCACCAATA TCACATTTGG TTTCAGAACA AGGGATGCAA ATGTAATAAT ATTGCATGCA 
    GAAAAAGAGC CTGAATTTCT TAATATTAGC ATTCAAGATT CCAGATTATT CTTTCAATTG 
    CAAAGTGGCA ACAGCTTTTA TATGCTAAGT CTGACAAGTT TGCAGTCAGT GAATGATGGC 
    ACATGGCACG AAGTGACCCT TTCCATGACA GACCCACTGT CCCAGACCTC CAGGTGGCAA 
    ATGGAAGTGG ACAACGAAAC ACCTTTTGTG ACCAGCACAA TTGCTACTGG AAGCCTCAAC 
    TTTTTGAAGG ATAATACAGA TATTTATGTG GGAGACAGAG CTATTGACAA TATAAAGGGC 
    CTGCAAGGGT GTCTAAGTAC AATAGAAATC GGAGGCATTT ATCTCTCTTA CTTTGAAAAT 
    GTTCATGGTT TCATTAATAA ACCTCAGGAA GAGCAATTTC TCAAAATCTC TACCAATTCA 
    GTGGTCACTG GCTGTTTGCA GTTAAATGTC TGCAACTCCA ACCCCTGTTT GCATGGAGGA 
    AACTGTGAAG ACATCTATAG CTCTTATCAT TGCTCCTGTC CCTTGGGATG GTCAGGGAAA 
    CACTGTGAAC TCAACATCGA TGAATGCTTT TCAAACCCCT GTATCCATGG CAACTGCTCT 
    GACAGAGTTG CAGCCTACCA CTGCACATGT GAGCCTGGAT ACACTGGTGT GAACTGTGAA 
    GTGGATATAG ACAACTGCCA GAGTCACCAG TGTGCAAATG GAGCCACCTG CATTAGTCAT 
    ACTAATGGCT ATTCTTGCCT CTGTTTTGGA AATTTTACAG GAAAATTTTG CAGACAGAGC 
    AGATTACCCT CAACAGTCTG TGGGAATGAG AAGACAAATC TCACTTGCTA CAATGGAGGC 
    AACTGCACAG AGTTCCAGAC TGAATTAAAA TGTATGTGCC GGCCAGGTTT TACTGGAGAA 
    TGGTGTGAAA AGGACATTGA TGAGTGTGCC TCTGATCCGT GTGTCAATGG AGGTCTGTGC 
    CAGGACTTAC TCAACAAATT CCAGTGCCTC TGTGATGTTG CCTTTGCTGG CGAGCGCTGC 
    GAGGTGGACT TGGCAGATGA CTTGATCTCC GACATTTTCA CCACTATTGG CTCAGTGACT 
    GTCGCCTTGT TACTGATCCT CTTGCTGGCC ATTGTTGCTT CTGTTGTCAC CTCCAACAAA 
    AGGGCAACTC AGGGAACCTA CAGCCCCAGC CGTCAGGAGA AGGAGGGCTC CCGAGTGGAA 
    ATGTGGAACT TGATGCCACC CCCTGCAATG GAGAGACTGA TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CRB1 ( XM_011509367.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507669.1 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OHu66167
    Clone ID Related Accession (Same CDS sequence) XM_011509367.1
    Accession Version XM_011509367.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA 
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC 
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA 
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT 
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA 
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT 
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG 
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC 
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA 
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC 
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG 
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA 
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA 
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC 
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT 
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC 
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG 
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT 
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA 
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT 
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT 
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC 
    CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT 
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA 
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG 
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC 
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC 
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT 
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA 
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG 
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA 
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT 
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT 
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG 
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC 
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC 
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT 
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC 
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC 
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT 
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG 
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA 
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT 
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT 
    GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA 
    GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG 
    CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA 
    ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC 
    AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT 
    ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG 
    CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC 
    ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT 
    TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT 
    TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA 
    GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT 
    CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA 
    AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT 
    TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA 
    TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC 
    ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT 
    CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT 
    TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG 
    AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA 
    TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGTT GGCAGATGAC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011507669.1
    CDS135..4028
    Translation

    Target ORF information:

    RefSeq Version XM_011509367.1
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011509367.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA 
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC 
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA 
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT 
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA 
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT 
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG 
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC 
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA 
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC 
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG 
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA 
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA 
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC 
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT 
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC 
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG 
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT 
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA 
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT 
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT 
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC 
    CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT 
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA 
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG 
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC 
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC 
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT 
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA 
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG 
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA 
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT 
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT 
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG 
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC 
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC 
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT 
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC 
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC 
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT 
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG 
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA 
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT 
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT 
    GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA 
    GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG 
    CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA 
    ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC 
    AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT 
    ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG 
    CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC 
    ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT 
    TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT 
    TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA 
    GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT 
    CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA 
    AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT 
    TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA 
    TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC 
    ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT 
    CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT 
    TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG 
    AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA 
    TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGTT GGCAGATGAC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CRB1 ( NM_001257965.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244894.1 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID OHu22677
    Clone ID Related Accession (Same CDS sequence) NM_001257965.1
    Accession Version NM_001257965.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302826.1, AK289733.1, AL136322.24 and AY043325.1. Transcript Variant: This variant (3) contains three noncoding exons in place of the first exon and contains an alternate in-frame exon compared to variant 1. The resulting isoform (3) is shorter at the N- terminus and contains an alternate internal segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302826.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968832, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGACC CTTGCTTCTC CAATCCCTGT CAAGGAAGTG CCACTTGTGT GAACACCCCA 
    GGAGAAAGGA GCTTTCTGTG CAAATGTCCT CCTGGGTACA GTGGGACAAT CTGTGAAACT 
    ACCATTGGTT CCTGTGGCAA GAACTCCTGC CAACATGGAG GTATTTGCCA TCAGGACCCT 
    ATTTATCCTG TCTGCATCTG CCCTGCTGGA TATGCTGGAA GATTCTGTGA GATAGATCAC 
    GATGAGTGTG CTTCCAGCCC TTGCCAAAAT GGGGCCGTGT GCCAGGATGG AATTGATGGT 
    TACTCCTGCT TCTGTGTCCC AGGATATCAA GGCAGACACT GCGACTTGGA AGTGGATGAA 
    TGTGCTTCAG ATCCCTGCAA GAACGAGGCT ACATGCCTCA ATGAAATAGG AAGATATACT 
    TGTATCTGTC CCCACAATTA TTCTGGTGTA AACTGTGAAT TGGAAATTGA CGAATGTTGG 
    TCCCAGCCTT GTTTAAATGG TGCAACTTGT CAGGATGCTC TGGGGGCCTA TTTCTGCGAC 
    TGTGCCCCTG GATTCCTGGG GGATCACTGT GAACTCAACA CTGATGAGTG TGCCAGTCAA 
    CCTTGTCTCC ATGGAGGGCT GTGTGTGGAT GGAGAAAACA GATATAGCTG TAACTGCACG 
    GGTAGTGGAT TCACAGGGAC ACACTGTGAG ACCTTGATGC CTCTTTGTTG GTCAAAACCT 
    TGTCACAATA ATGCTACATG TGAGGACAGT GTTGACAATT ACACTTGTCA CTGCTGGCCT 
    GGATACACAG GTGCCCAGTG TGAGATCGAC CTCAATGAAT GCAATAGTAA CCCCTGCCAG 
    TCCAATGGGG AATGTGTGGA GCTGTCCTCA GAGAAACAAT ATGGACGCAT CACTGGACTG 
    CCTTCTTCTT TCAGCTACCA TGAAGCCTCA GGTTATGTCT GTATCTGTCA GCCTGGATTC 
    ACAGGAATCC ACTGCGAAGA AGACGTCAAT GAATGTTCTT CAAACCCTTG CCAAAATGGT 
    GGTACTTGTG AGAACTTGCC TGGGAATTAT ACTTGCCATT GCCCATTTGA TAACCTTTCT 
    AGAACTTTTT ATGGAGGAAG GGACTGTTCT GATATTCTCC TGGGCTGTAC CCATCAGCAA 
    TGTCTAAATA ATGGAACATG CATCCCTCAC TTCCAAGATG GCCAGCATGG ATTCAGCTGC 
    CTGTGTCCAT CTGGCTACAC CGGGTCCCTG TGTGAAATCG CAACCACACT TTCATTTGAG 
    GGCGATGGCT TCCTGTGGGT CAAAAGTGGC TCAGTGACAA CCAAGGGCTC AGTTTGTAAC 
    ATAGCCCTCA GGTTTCAGAC TGTTCAGCCA ATGGCTCTTC TACTTTTCCG AAGCAACAGG 
    GATGTGTTTG TGAAGCTGGA GCTGCTAAGT GGCTACATTC ACTTATCAAT TCAGGTCAAT 
    AATCAGTCAA AGGTGCTTCT GTTCATTTCC CACAACACCA GCGATGGAGA GTGGCATTTC 
    GTGGAGGTAA TATTTGCAGA GGCTGTGACC CTTACCTTAA TCGACGACTC CTGTAAGGAG 
    AAATGCATCG CGAAAGCTCC TACTCCACTT GAAAGTGATC AATCAATATG TGCTTTTCAG 
    AACTCCTTTT TGGGTGGTTT ACCAGTGGGA ATGACCAGCA ATGGTGTTGC TCTGCTTAAC 
    TTCTATAATA TGCCATCCAC ACCTTCGTTT GTAGGCTGTC TCCAAGACAT TAAAATTGAT 
    TGGAATCACA TTACCCTGGA GAACATCTCG TCTGGCTCAT CATTAAATGT CAAGGCAGGC 
    TGTGTGAGAA AGGATTGGTG TGAAAGCCAA CCTTGTCAAA GCAGAGGACG CTGCATCAAC 
    TTGTGGCTGA GTTACCAGTG TGACTGCCAC AGGCCCTATG AAGGCCCCAA CTGTCTGAGA 
    GAGTATGTGG CAGGCAGATT TGGCCAGGAT GACTCCACTG GTTATGTCAT CTTTACTCTT 
    GATGAGAGCT ATGGAGACAC CATCAGCCTC TCCATGTTTG TCCGAACGCT TCAACCATCA 
    GGCTTACTTC TAGCTTTGGA AAACAGCACT TATCAATATA TCCGTGTCTG GCTAGAGCGC 
    GGCAGACTAG CAATGCTGAC TCCAAACTCT CCCAAATTAG TAGTAAAATT TGTTCTTAAT 
    GATGGAAATG TCCACTTGAT ATCTTTGAAA ATCAAGCCAT ATAAAATTGA ACTGTATCAG 
    TCTTCACAAA ACCTAGGATT TATTTCTGCT TCTACGTGGA AAATCGAAAA GGGAGATGTC 
    ATCTACATTG GTGGCCTACC TGACAAGCAA GAGACTGAAC TTAATGGTGG ATTCTTCAAA 
    GGCTGTATCC AAGATGTAAG ACTAAACAAC CAAAATCTGG AATTCTTTCC AAATCCAACA 
    AACAATGCAT CTCTCAATCC AGTTCTTGTC AATGTAACCC AAGGCTGTGC TGGAGACAAC 
    AGCTGCAAGA GGCAGACCAA TGTGGGAAGG GCACTCACTG AGTTGGGATC CAGAGGACCT 
    AAGTACCAAG TTTCACTGTT TCGCTTCTGT GTAGGATCTT GGGCAACTGG AAACACCTTC 
    TTTTTATCAT CTATAAAACC AGGATCCAAC CCCTGTCACA ATGGAGGTGT TTGCCATTCC 
    CGGTGGGATG ACTTCTCCTG TTCCTGTCCT GCCCTCACAA GTGGGAAAGC CTGTGAGGAG 
    GTTCAGTGGT GTGGATTCAG CCCGTGTCCT CACGGAGCCC AGTGCCAGCC GGTGCTTCAA 
    GGATTTGAAT GTATTGCAAA TGCTGTTTTT AATGGACAAA GCGGTCAAAT ATTATTCAGA 
    AGCAATGGGA ATATTACCAG AGAACTCACC AATATCACAT TTGGTTTCAG AACAAGGGAT 
    GCAAATGTAA TAATATTGCA TGCAGAAAAA GAGCCTGAAT TTCTTAATAT TAGCATTCAA 
    GATTCCAGAT TATTCTTTCA ATTGCAAAGT GGCAACAGCT TTTATATGCT AAGTCTGACA 
    AGTTTGCAGT CAGTGAATGA TGGCACATGG CACGAAGTGA CCCTTTCCAT GACAGACCCA 
    CTGTCCCAGA CCTCCAGGTG GCAAATGGAA GTGGACAACG AAACACCTTT TGTGACCAGC 
    ACAATTGCTA CTGGAAGCCT CAACTTTTTG AAGGATAATA CAGATATTTA TGTGGGAGAC 
    AGAGCTATTG ACAATATAAA GGGCCTGCAA GGGTGTCTAA GTACAATAGA AATCGGAGGC 
    ATTTATCTCT CTTACTTTGA AAATGTTCAT GGTTTCATTA ATAAACCTCA GGAAGAGCAA 
    TTTCTCAAAA TCTCTACCAA TTCAGTGGTC ACTGGCTGTT TGCAGTTAAA TGTCTGCAAC 
    TCCAACCCCT GTTTGCATGG AGGAAACTGT GAAGACATCT ATAGCTCTTA TCATTGCTCC 
    TGTCCCTTGG GATGGTCAGG GAAACACTGT GAACTCAACA TCGATGAATG CTTTTCAAAC 
    CCCTGTATCC ATGGCAACTG CTCTGACAGA GTTGCAGCCT ACCACTGCAC ATGTGAGCCT 
    GGATACACTG GTGTGAACTG TGAAGTGGAT ATAGACAACT GCCAGAGTCA CCAGTGTGCA 
    AATGGAGCCA CCTGCATTAG TCATACTAAT GGCTATTCTT GCCTCTGTTT TGGAAATTTT 
    ACAGGAAAAT TTTGCAGACA GAGCAGATTA CCCTCAACAG TCTGTGGGAA TGAGAAGACA 
    AATCTCACTT GCTACAATGG AGGCAACTGC ACAGAGTTCC AGACTGAATT AAAATGTATG 
    TGCCGGCCAG GTTTTACTGG AGAATGGTGT GAAAAGGACA TTGATGAGTG TGCCTCTGAT 
    CCGTGTGTCA ATGGAGGTCT GTGCCAGGAC TTACTCAACA AATTCCAGTG CCTCTGTGAT 
    GTTGCCTTTG CTGGCGAGCG CTGCGAGGTG GACTTGGCAG ATGACTTGAT CTCCGACATT 
    TTCACCACTA TTGGCTCAGT GACTGTCGCC TTGTTACTGA TCCTCTTGCT GGCCATTGTT 
    GCTTCTGTTG TCACCTCCAA CAAAAGGGCA ACTCAGGGAA CCTACAGCCC CAGCCGTCAG 
    GAGAAGGAGG GCTCCCGAGT GGAAATGTGG AACTTGATGC CACCCCCTGC AATGGAGAGA 
    CTGATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001244894.1
    CDS340..4488
    Translation

    Target ORF information:

    RefSeq Version NM_001257965.1
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001257965.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGACC CTTGCTTCTC CAATCCCTGT CAAGGAAGTG CCACTTGTGT GAACACCCCA 
    GGAGAAAGGA GCTTTCTGTG CAAATGTCCT CCTGGGTACA GTGGGACAAT CTGTGAAACT 
    ACCATTGGTT CCTGTGGCAA GAACTCCTGC CAACATGGAG GTATTTGCCA TCAGGACCCT 
    ATTTATCCTG TCTGCATCTG CCCTGCTGGA TATGCTGGAA GATTCTGTGA GATAGATCAC 
    GATGAGTGTG CTTCCAGCCC TTGCCAAAAT GGGGCCGTGT GCCAGGATGG AATTGATGGT 
    TACTCCTGCT TCTGTGTCCC AGGATATCAA GGCAGACACT GCGACTTGGA AGTGGATGAA 
    TGTGCTTCAG ATCCCTGCAA GAACGAGGCT ACATGCCTCA ATGAAATAGG AAGATATACT 
    TGTATCTGTC CCCACAATTA TTCTGGTGTA AACTGTGAAT TGGAAATTGA CGAATGTTGG 
    TCCCAGCCTT GTTTAAATGG TGCAACTTGT CAGGATGCTC TGGGGGCCTA TTTCTGCGAC 
    TGTGCCCCTG GATTCCTGGG GGATCACTGT GAACTCAACA CTGATGAGTG TGCCAGTCAA 
    CCTTGTCTCC ATGGAGGGCT GTGTGTGGAT GGAGAAAACA GATATAGCTG TAACTGCACG 
    GGTAGTGGAT TCACAGGGAC ACACTGTGAG ACCTTGATGC CTCTTTGTTG GTCAAAACCT 
    TGTCACAATA ATGCTACATG TGAGGACAGT GTTGACAATT ACACTTGTCA CTGCTGGCCT 
    GGATACACAG GTGCCCAGTG TGAGATCGAC CTCAATGAAT GCAATAGTAA CCCCTGCCAG 
    TCCAATGGGG AATGTGTGGA GCTGTCCTCA GAGAAACAAT ATGGACGCAT CACTGGACTG 
    CCTTCTTCTT TCAGCTACCA TGAAGCCTCA GGTTATGTCT GTATCTGTCA GCCTGGATTC 
    ACAGGAATCC ACTGCGAAGA AGACGTCAAT GAATGTTCTT CAAACCCTTG CCAAAATGGT 
    GGTACTTGTG AGAACTTGCC TGGGAATTAT ACTTGCCATT GCCCATTTGA TAACCTTTCT 
    AGAACTTTTT ATGGAGGAAG GGACTGTTCT GATATTCTCC TGGGCTGTAC CCATCAGCAA 
    TGTCTAAATA ATGGAACATG CATCCCTCAC TTCCAAGATG GCCAGCATGG ATTCAGCTGC 
    CTGTGTCCAT CTGGCTACAC CGGGTCCCTG TGTGAAATCG CAACCACACT TTCATTTGAG 
    GGCGATGGCT TCCTGTGGGT CAAAAGTGGC TCAGTGACAA CCAAGGGCTC AGTTTGTAAC 
    ATAGCCCTCA GGTTTCAGAC TGTTCAGCCA ATGGCTCTTC TACTTTTCCG AAGCAACAGG 
    GATGTGTTTG TGAAGCTGGA GCTGCTAAGT GGCTACATTC ACTTATCAAT TCAGGTCAAT 
    AATCAGTCAA AGGTGCTTCT GTTCATTTCC CACAACACCA GCGATGGAGA GTGGCATTTC 
    GTGGAGGTAA TATTTGCAGA GGCTGTGACC CTTACCTTAA TCGACGACTC CTGTAAGGAG 
    AAATGCATCG CGAAAGCTCC TACTCCACTT GAAAGTGATC AATCAATATG TGCTTTTCAG 
    AACTCCTTTT TGGGTGGTTT ACCAGTGGGA ATGACCAGCA ATGGTGTTGC TCTGCTTAAC 
    TTCTATAATA TGCCATCCAC ACCTTCGTTT GTAGGCTGTC TCCAAGACAT TAAAATTGAT 
    TGGAATCACA TTACCCTGGA GAACATCTCG TCTGGCTCAT CATTAAATGT CAAGGCAGGC 
    TGTGTGAGAA AGGATTGGTG TGAAAGCCAA CCTTGTCAAA GCAGAGGACG CTGCATCAAC 
    TTGTGGCTGA GTTACCAGTG TGACTGCCAC AGGCCCTATG AAGGCCCCAA CTGTCTGAGA 
    GAGTATGTGG CAGGCAGATT TGGCCAGGAT GACTCCACTG GTTATGTCAT CTTTACTCTT 
    GATGAGAGCT ATGGAGACAC CATCAGCCTC TCCATGTTTG TCCGAACGCT TCAACCATCA 
    GGCTTACTTC TAGCTTTGGA AAACAGCACT TATCAATATA TCCGTGTCTG GCTAGAGCGC 
    GGCAGACTAG CAATGCTGAC TCCAAACTCT CCCAAATTAG TAGTAAAATT TGTTCTTAAT 
    GATGGAAATG TCCACTTGAT ATCTTTGAAA ATCAAGCCAT ATAAAATTGA ACTGTATCAG 
    TCTTCACAAA ACCTAGGATT TATTTCTGCT TCTACGTGGA AAATCGAAAA GGGAGATGTC 
    ATCTACATTG GTGGCCTACC TGACAAGCAA GAGACTGAAC TTAATGGTGG ATTCTTCAAA 
    GGCTGTATCC AAGATGTAAG ACTAAACAAC CAAAATCTGG AATTCTTTCC AAATCCAACA 
    AACAATGCAT CTCTCAATCC AGTTCTTGTC AATGTAACCC AAGGCTGTGC TGGAGACAAC 
    AGCTGCAAGA GGCAGACCAA TGTGGGAAGG GCACTCACTG AGTTGGGATC CAGAGGACCT 
    AAGTACCAAG TTTCACTGTT TCGCTTCTGT GTAGGATCTT GGGCAACTGG AAACACCTTC 
    TTTTTATCAT CTATAAAACC AGGATCCAAC CCCTGTCACA ATGGAGGTGT TTGCCATTCC 
    CGGTGGGATG ACTTCTCCTG TTCCTGTCCT GCCCTCACAA GTGGGAAAGC CTGTGAGGAG 
    GTTCAGTGGT GTGGATTCAG CCCGTGTCCT CACGGAGCCC AGTGCCAGCC GGTGCTTCAA 
    GGATTTGAAT GTATTGCAAA TGCTGTTTTT AATGGACAAA GCGGTCAAAT ATTATTCAGA 
    AGCAATGGGA ATATTACCAG AGAACTCACC AATATCACAT TTGGTTTCAG AACAAGGGAT 
    GCAAATGTAA TAATATTGCA TGCAGAAAAA GAGCCTGAAT TTCTTAATAT TAGCATTCAA 
    GATTCCAGAT TATTCTTTCA ATTGCAAAGT GGCAACAGCT TTTATATGCT AAGTCTGACA 
    AGTTTGCAGT CAGTGAATGA TGGCACATGG CACGAAGTGA CCCTTTCCAT GACAGACCCA 
    CTGTCCCAGA CCTCCAGGTG GCAAATGGAA GTGGACAACG AAACACCTTT TGTGACCAGC 
    ACAATTGCTA CTGGAAGCCT CAACTTTTTG AAGGATAATA CAGATATTTA TGTGGGAGAC 
    AGAGCTATTG ACAATATAAA GGGCCTGCAA GGGTGTCTAA GTACAATAGA AATCGGAGGC 
    ATTTATCTCT CTTACTTTGA AAATGTTCAT GGTTTCATTA ATAAACCTCA GGAAGAGCAA 
    TTTCTCAAAA TCTCTACCAA TTCAGTGGTC ACTGGCTGTT TGCAGTTAAA TGTCTGCAAC 
    TCCAACCCCT GTTTGCATGG AGGAAACTGT GAAGACATCT ATAGCTCTTA TCATTGCTCC 
    TGTCCCTTGG GATGGTCAGG GAAACACTGT GAACTCAACA TCGATGAATG CTTTTCAAAC 
    CCCTGTATCC ATGGCAACTG CTCTGACAGA GTTGCAGCCT ACCACTGCAC ATGTGAGCCT 
    GGATACACTG GTGTGAACTG TGAAGTGGAT ATAGACAACT GCCAGAGTCA CCAGTGTGCA 
    AATGGAGCCA CCTGCATTAG TCATACTAAT GGCTATTCTT GCCTCTGTTT TGGAAATTTT 
    ACAGGAAAAT TTTGCAGACA GAGCAGATTA CCCTCAACAG TCTGTGGGAA TGAGAAGACA 
    AATCTCACTT GCTACAATGG AGGCAACTGC ACAGAGTTCC AGACTGAATT AAAATGTATG 
    TGCCGGCCAG GTTTTACTGG AGAATGGTGT GAAAAGGACA TTGATGAGTG TGCCTCTGAT 
    CCGTGTGTCA ATGGAGGTCT GTGCCAGGAC TTACTCAACA AATTCCAGTG CCTCTGTGAT 
    GTTGCCTTTG CTGGCGAGCG CTGCGAGGTG GACTTGGCAG ATGACTTGAT CTCCGACATT 
    TTCACCACTA TTGGCTCAGT GACTGTCGCC TTGTTACTGA TCCTCTTGCT GGCCATTGTT 
    GCTTCTGTTG TCACCTCCAA CAAAAGGGCA ACTCAGGGAA CCTACAGCCC CAGCCGTCAG 
    GAGAAGGAGG GCTCCCGAGT GGAAATGTGG AACTTGATGC CACCCCCTGC AATGGAGAGA 
    CTGATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CRB1 ( XM_011509365.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507667.1 Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OHu66165
    Clone ID Related Accession (Same CDS sequence) XM_011509365.2
    Accession Version XM_011509365.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4050bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509365.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA 
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC 
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA 
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT 
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA 
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT 
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG 
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC 
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA 
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC 
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG 
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA 
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA 
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC 
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT 
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC 
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG 
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT 
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA 
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT 
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT 
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC 
    CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT 
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA 
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG 
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC 
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC 
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT 
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA 
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG 
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA 
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT 
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT 
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG 
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC 
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC 
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT 
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC 
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC 
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT 
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG 
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA 
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT 
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT 
    GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA 
    GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG 
    CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA 
    ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC 
    AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT 
    ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG 
    CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC 
    ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT 
    TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT 
    TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA 
    GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT 
    CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA 
    AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT 
    TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA 
    TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC 
    ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT 
    CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT 
    TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG 
    AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA 
    TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG 
    TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG 
    TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACATCTC TGGGGAAGAA 
    AAAGGAAATA AAAACCATCT CAATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011507667.1
    CDS135..4184
    Translation

    Target ORF information:

    RefSeq Version XM_011509365.2
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011509365.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA 
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC 
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA 
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT 
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA 
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT 
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG 
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC 
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA 
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC 
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG 
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA 
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA 
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC 
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT 
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC 
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG 
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT 
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA 
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT 
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT 
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC 
    CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT 
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA 
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG 
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC 
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC 
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT 
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA 
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG 
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA 
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT 
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT 
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG 
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC 
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC 
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT 
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC 
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC 
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT 
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG 
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA 
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT 
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT 
    GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA 
    GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG 
    CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA 
    ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC 
    AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT 
    ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG 
    CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC 
    ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT 
    TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT 
    TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA 
    GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT 
    CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA 
    AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT 
    TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA 
    TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC 
    ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT 
    CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT 
    TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG 
    AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA 
    TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG 
    TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG 
    TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACATCTC TGGGGAAGAA 
    AAAGGAAATA AAAACCATCT CAATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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