DHX30 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DHX30
Entrez Gene ID	22907
Full Name	DExH-box helicase 30
Synonyms	DDX30,NEDMIAL,RETCOR
General protein information	Preferred Names DExH-box helicase 30 Names putative ATP-dependent RNA helicase DHX30 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 30 DEAH (Asp-Glu-Ala-His) box helicase 30 DEAH (Asp-Glu-Ala-His) box polypeptide 30 DEAH box protein 30 DEAH-box helicase 30 retina co-repressor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.31
Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The family member encoded by this gene is a mitochondrial nucleoid protein that associates with mitochondrial DNA. It has also been identified as a component of a transcriptional repressor complex that functions in retinal development, and it is required to optimize the function of the zinc-finger antiviral protein. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013].
Disorder MIM:	616423

mRNA and Protein(s)

mRNA	Protein	Name
NM_138615.2	NP_619520.1	putative ATP-dependent RNA helicase DHX30 isoform 1
XM_011533493.1	XP_011531795.1	putative ATP-dependent RNA helicase DHX30 isoform X3
XM_011533494.2	XP_011531796.1	putative ATP-dependent RNA helicase DHX30 isoform X4
XM_011533494.3	XP_011531796.1	ATP-dependent RNA helicase DHX30 isoform X4
XM_017005915.1	XP_016861404.1	ATP-dependent RNA helicase DHX30 isoform X6
XM_017005916.2	XP_016861405.1	ATP-dependent RNA helicase DHX30 isoform X7
XM_011533490.1	XP_011531792.1	putative ATP-dependent RNA helicase DHX30 isoform X1
XM_017005916.1	XP_016861405.1	putative ATP-dependent RNA helicase DHX30 isoform X7
NM_014966.3	NP_055781.2	ATP-dependent RNA helicase DHX30 isoform 2
XM_011533490.2	XP_011531792.1	ATP-dependent RNA helicase DHX30 isoform X1
XM_024453405.1	XP_024309173.1	ATP-dependent RNA helicase DHX30 isoform X3
XM_011533497.2	XP_011531799.1	ATP-dependent RNA helicase DHX30 isoform X5
XM_006713033.1	XP_006713096.1	ATP-dependent RNA helicase DHX30 isoform X6
XM_017005917.1	XP_016861406.1	ATP-dependent RNA helicase DHX30 isoform X8
XM_011533494.1	XP_011531796.1	putative ATP-dependent RNA helicase DHX30 isoform X1
NM_138615.3	NP_619520.1	ATP-dependent RNA helicase DHX30 isoform 1
XM_017005914.1	XP_016861403.1	ATP-dependent RNA helicase DHX30 isoform X2
XM_011533495.2	XP_011531797.1	ATP-dependent RNA helicase DHX30 isoform X4
XM_011533498.1	XP_011531800.1	ATP-dependent RNA helicase DHX30 isoform X5
XM_011533492.2	XP_011531794.1	putative ATP-dependent RNA helicase DHX30 isoform X1
XM_011533496.1	XP_011531798.1	putative ATP-dependent RNA helicase DHX30 isoform X5
XM_011533495.1	XP_011531797.1	putative ATP-dependent RNA helicase DHX30 isoform X4
XM_011533491.1	XP_011531793.1	putative ATP-dependent RNA helicase DHX30 isoform X1
NM_001330990.1	NP_001317919.1	ATP-dependent RNA helicase DHX30 isoform 4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DHX30	NP_001070003.1
Xenopus tropicalis (tropical clawed frog)	dhx30	NP_001107694.1
Homo sapiens (human)	DHX30	NP_619520.1
Canis lupus familiaris (dog)	DHX30	XP_005632658.1
Rattus norvegicus (Norway rat)	Dhx30	NP_001013267.1
Gallus gallus (chicken)	DHX30	NP_001012869.1
	LOC711178	XP_001113231.2
Mus musculus (house mouse)	Dhx30	NP_001239612.1
Pan troglodytes (chimpanzee)	DHX30	XP_001155300.2
Danio rerio (zebrafish)	dhx30	XP_005171140.1

Gene Ontology
Bibliography

Related articles in PubMed

De Novo Missense Mutations in DHX30 Impair Global Translation and Cause a Neurodevelopmental Disorder.
Lessel D, Schob C, K?ry S, Reijnders MRF, Harel T, Eldomery MK, Coban-Akdemir Z, Denecke J, Edvardson S, Colin E, Stegmann APA, Gerkes EH, Tessarech M, Bonneau D, Barth M, Besnard T, Cogn? B, Revah-Politi A, Strom TM, Rosenfeld JA, Yang Y, Posey JE, Immken L, Oundjian N, Helbig KL, Meeks N, Zegar K, Morton J, , Schieving JH, Claasen A, Huentelman M, Narayanan V, Ramsey K, , Brunner HG, Elpeleg O, Mercier S, B?zieau S, Kubisch C, Kleefstra T, Kindler S, Lupski JR, Kreienkamp HJ
American journal of human genetics101(5)716-724(2017 Nov)

Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.
Antonicka H, Shoubridge EA
Cell reports(2015 Feb)

In pursuit of synthetic modulators for the orphan retina-specific nuclear receptor NR2E3.
Qin Q, Knapinska A, Dobri N, Madoux F, Chase P, Hodder P, Petrukhin K
Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics29(3)298-309(2013 Apr)

Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS.
Varjosalo M, Sacco R, Stukalov A, van Drogen A, Planyavsky M, Hauri S, Aebersold R, Bennett KL, Colinge J, Gstaiger M, Superti-Furga G
Nature methods10(4)307-14(2013 Apr)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Provide molecular insight into how DHX30 dysfunction might cause a neurodevelopmental disorder.
Title: De Novo Missense Mutations in DHX30 Impair Global Translation and Cause a Neurodevelopmental Disorder.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Here, we provide evidence that overexpression of an RNA helicase named DHX30 enhances HIV-1 gene expression, but leads to the generation of viruses that package significantly low levels of viral RNA and exhibit severely decreased infectivity.
Title: The packaging of human immunodeficiency virus type 1 RNA is restricted by overexpression of an RNA helicase DHX30.

Identifies DHX30 as a nucleoid protein.
Title: The layered structure of human mitochondrial DNA nucleoids.

The following DHX30 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHX30 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09083	NM_014966.3 Latest version!	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$69.30 $99.00
OHu09108	NM_138615.3 Latest version!	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$69.30 $99.00
OHu65127	NM_001330990.1 Latest version!	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
OHu09083	XM_017005917.1 Latest version!	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$69.30 $99.00
OHu09108	XM_011533495.2 Latest version!	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$69.30 $99.00
OHu09108	XM_011533494.3 Latest version!	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$69.30 $99.00
OHu65127	XM_011533498.1 Latest version!	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
OHu65127	XM_011533497.2 Latest version!	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
OHu65127	XM_011533496.1 Latest version!	Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
OHu93898	XM_017005914.1 Latest version!	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu93899	XM_017005916.2 Latest version!	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu37057	XM_006713033.1 Latest version!	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu37057	XM_017005915.1 Latest version!	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu65125	XM_011533492.2 Latest version!	Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu65125	XM_011533491.1 Latest version!	Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu65125	XM_011533490.2 Latest version!	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu65126	XM_011533493.1 Latest version!	Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu65126	XM_024453405.1 Latest version!	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OHu09108	XM_011533494.2	Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$69.30 $99.00
OHu93899	XM_017005916.1	Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu65125	XM_011533490.1	Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu09108	XM_011533494.1	Homo sapiens DEAH (Asp-Glu-Ala-His) box helicase 30 (DHX30), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$69.30 $99.00
OHu09108	NM_138615.2	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$69.30 $99.00
OHu09108	XM_011533495.1	Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DHX30 ( NM_014966.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055781.2 Homo sapiens DExH-box helicase 30 (DHX30), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09083
Clone ID Related Accession (Same CDS sequence)	NM_014966.3 , XM_017005917.1
Accession Version	NM_014966.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX30 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC047335.1, BC015029.1 and CD368247.1. On Apr 9, 2009 this sequence version replaced NM_014966.2. Transcript Variant: This variant (2) differs in its 5' UTR and uses an alternate translational start codon, compared to variant 1. The resulting isoform (2) has a distinct and shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.37342.1, AK291266.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 18063578 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCTT CTAGGGACCT ATTAAAAGAG TTCCCACAGC CCAAAAATCT TCTCAACAGT 
GTGATTGGAA GAGCCCTCGG CATCTCACAT GCAAAAGACA AACTAGTCTA CGTGCACACA 
AATGGACCGA AGAAAAAGAA AGTCACACTG CACATAAAAT GGCCCAAGAG CGTGGAGGTA 
GAAGGCTATG GCAGCAAGAA GATCGATGCT GAGCGGCAGG CTGCAGCTGC AGCCTGCCAG 
CTGTTCAAGG GTTGGGGTCT GCTAGGTCCC CGGAATGAGT TGTTTGACGC AGCCAAATAC 
CGAGTGCTAG CTGATCGCTT TGGCTCCCCT GCCGACAGCT GGTGGCGTCC GGAACCCACC 
ATGCCCCCTA CTTCCTGGCG GCAGCTGAAT CCAGAGAGTA TTCGACCAGG GGGACCTGGG 
GGCCTATCCC GCTCTTTAGG CCGGGAAGAA GAGGAGGACG AGGAGGAAGA GCTAGAAGAA 
GGGACCATAG ATGTTACCGA CTTCTTGTCC ATGACCCAGC AGGATTCCCA CGCTCCACTC 
AGGGACTCAA GGGGGAGTTC CTTTGAGATG ACAGATGACG ACAGTGCCAT TAGGGCTCTG 
ACCCAGTTTC CACTTCCCAA GAACCTTCTG GCCAAGGTGA TTCAGATTGC AACGTCATCC 
TCCACAGCTA AGAACCTCAT GCAGTTCCAT ACTGTGGGCA CCAAGACCAA GCTGTCTACA 
CTCACCCTGC TCTGGCCCTG CCCCATGACC TTTGTTGCCA AAGGGCGCCG CAAAGCAGAG 
GCTGAGAATA AGGCGGCAGC CTTGGCCTGC AAGAAACTGA AGAGCCTGGG CCTGGTGGAC 
AGGAACAACG AACCGCTTAC ACACGCCATG TATAACCTGG CCTCTTTGCG TGAGCTGGGT 
GAGACCCAGC GCCGACCATG CACCATCCAG GTGCCCGAGC CCATCCTCCG CAAGATAGAG 
ACCTTCCTGA ACCATTACCC TGTGGAGAGT TCATGGATCG CCCCAGAACT CCGGCTGCAG 
AGTGATGACA TCTTGCCCTT GGGCAAGGAC TCAGGGCCTC TGAGTGACCC TATCACAGGC 
AAGCCCTATG TGCCCCTGTT GGAAGCAGAG GAGGTACGTC TCAGCCAGAG TCTGCTAGAA 
CTGTGGCGGC GGCGAGGGCC GGTCTGGCAG GAGGCCCCCC AGCTACCTGT GGACCCACAT 
CGGGACACCA TCCTCAACGC CATTGAGCAG CACCCGGTGG TGGTCATCTC TGGGGACACG 
GGCTGTGGGA AGACCACGCG CATCCCCCAG CTGTTGCTGG AGCGCTATGT GACCGAGGGC 
CGAGGTGCCC GCTGCAATGT TATCATCACC CAACCTCGCC GCATCTCTGC TGTGTCTGTG 
GCACAGCGGG TCAGCCACGA ACTGGGCCCC TCCCTGCGCC GGAATGTGGG CTTCCAGGTG 
CGGTTGGAAA GTAAGCCCCC ATCCCGAGGC GGGGCCCTGC TCTTCTGCAC TGTGGGTATC 
CTGCTGCGTA AGCTGCAGAG CAACCCCAGC CTGGAGGGCG TGAGCCACGT CATCGTGGAT 
GAGGTGCATG AGCGGGACGT GAACACAGAC TTTCTGCTGA TCCTGCTCAA GGGCCTGCAG 
CGGCTCAACC CGGCCCTGCG GCTGGTGCTC ATGAGTGCCA CAGGGGACAA TGAGCGCTTC 
TCCCGATACT TTGGTGGCTG CCCCGTCATC AAGGTGCCTG GCTTCATGTA CCCAGTCAAG 
GAGCACTACC TAGAGGACAT CCTGGCCAAG TTGGGCAAGC ACCAGTACCT GCACCGGCAC 
CGGCACCATG AGTCTGAGGA TGAATGCGCA CTCGATTTGG ACCTTGTGAC TGATCTGGTT 
CTGCACATCG ATGCTCGCGG GGAACCAGGT GGGATCCTGT GCTTCCTGCC TGGGTGGCAG 
GAGATCAAAG GAGTGCAGCA GCGCCTCCAG GAGGCCCTGG GCATGCACGA GAGCAAGTAC 
CTCATCCTGC CAGTGCACTC CAACATCCCC ATGATGGATC AGAAGGCCAT ATTCCAGCAG 
CCTCCAGTTG GGGTGCGCAA GATTGTCTTG GCCACCAACA TTGCTGAGAC TTCCATCACA 
ATCAATGACA TCGTGCATGT GGTGGACAGT GGGCTGCACA AGGAAGAACG CTATGACCTG 
AAGACCAAGG TGTCCTGCCT GGAGACAGTG TGGGTATCAA GAGCCAATGT GATCCAGCGC 
CGGGGCCGGG CGGGCCGCTG CCAGTCCGGC TTTGCCTACC ACTTGTTCCC TCGAAGCCGG 
CTGGAGAAAA TGGTCCCTTT CCAAGTGCCA GAGATCCTGC GCACACCTCT TGAGAACCTG 
GTGCTGCAAG CGAAAATCCA CATGCCTGAG AAGACGGCGG TGGAGTTCCT GTCCAAGGCT 
GTGGACAGTC CAAACATCAA GGCAGTGGAC GAGGCTGTGA TCTTGCTCCA GGAGATCGGG 
GTGCTGGACC AGCGGGAGTA CCTGACTACC CTGGGGCAGC GCCTGGCTCA CATCTCCACC 
GACCCCCGGT TGGCCAAGGC CATTGTGTTG GCTGCCATCT TCCGTTGCCT GCACCCACTA 
CTGGTGGTCG TTTCCTGCCT CACCCGGGAC CCCTTCAGCA GCAGCCTACA GAACCGGGCA 
GAGGTGGACA AGGTGAAAGC ACTGTTGAGC CATGACAGCG GCAGTGACCA CCTGGCCTTT 
GTGCGGGCTG TCGCCGGCTG GGAGGAGGTG CTGCGTTGGC AGGACCGCAG CTCCCGGGAG 
AATTACCTGG AGGAAAACCT GCTGTACGCA CCCAGCCTGC GCTTCATCCA CGGACTCATC 
AAGCAGTTCT CAGAGAACAT TTATGAGGCC TTCCTGGTGG GGAAGCCCTC GGACTGCACC 
CTGGCCTCCG CCCAGTGCAA CGAGTACAGT GAGGAGGAGG AGCTGGTGAA GGGCGTGCTG 
ATGGCCGGCC TCTACCCCAA CCTCATCCAG GTGAGGCAGG GCAAGGTCAC CCGGCAGGGG 
AAGTTCAAGC CCAACAGCGT CACATATAGG ACCAAATCAG GCAACATCCT GCTGCACAAG 
TCGACCATTA ACAGGGAGGC CACACGGTTA CGGAGCCGAT GGCTGACGTA TTTCATGGCA 
GTCAAGTCCA ATGGCAGCGT CTTCGTCCGG GACTCCTCTC AGGTGCACCC GCTAGCTGTG 
CTGCTGCTGA CCGACGGGGA CGTGCACATC CGTGATGACG GGCGCCGGGC CACCATCTCA 
CTGAGCGACA GTGACCTGCT GCGGCTGGAG GGTGACTCGC GTACCGTGCG GCTGCTGAAG 
GAGCTGCGGC GGGCCCTGGG CCGCATGGTG GAGCGGAGCC TGCGCAGCGA GCTGGCTGCA 
CTTCCCCCCA GCGTACAGGA GGAGCACGGG CAGCTGCTTG CGCTACTGGC AGAGCTGCTG 
CGAGGACCCT GTGGCAGCTT TGATGTGCGC AAGACAGCTG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055781.2
CDS	573..4040
Translation	MAASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	NM_014966.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014966.3

ORF Insert Sequence:

ATGGCAGCTT CTAGGGACCT ATTAAAAGAG TTCCCACAGC CCAAAAATCT TCTCAACAGT 
GTGATTGGAA GAGCCCTCGG CATCTCACAT GCAAAAGACA AACTAGTCTA CGTGCACACA 
AATGGACCGA AGAAAAAGAA AGTCACACTG CACATAAAAT GGCCCAAGAG CGTGGAGGTA 
GAAGGCTATG GCAGCAAGAA GATCGATGCT GAGCGGCAGG CTGCAGCTGC AGCCTGCCAG 
CTGTTCAAGG GTTGGGGTCT GCTAGGTCCC CGGAATGAGT TGTTTGACGC AGCCAAATAC 
CGAGTGCTAG CTGATCGCTT TGGCTCCCCT GCCGACAGCT GGTGGCGTCC GGAACCCACC 
ATGCCCCCTA CTTCCTGGCG GCAGCTGAAT CCAGAGAGTA TTCGACCAGG GGGACCTGGG 
GGCCTATCCC GCTCTTTAGG CCGGGAAGAA GAGGAGGACG AGGAGGAAGA GCTAGAAGAA 
GGGACCATAG ATGTTACCGA CTTCTTGTCC ATGACCCAGC AGGATTCCCA CGCTCCACTC 
AGGGACTCAA GGGGGAGTTC CTTTGAGATG ACAGATGACG ACAGTGCCAT TAGGGCTCTG 
ACCCAGTTTC CACTTCCCAA GAACCTTCTG GCCAAGGTGA TTCAGATTGC AACGTCATCC 
TCCACAGCTA AGAACCTCAT GCAGTTCCAT ACTGTGGGCA CCAAGACCAA GCTGTCTACA 
CTCACCCTGC TCTGGCCCTG CCCCATGACC TTTGTTGCCA AAGGGCGCCG CAAAGCAGAG 
GCTGAGAATA AGGCGGCAGC CTTGGCCTGC AAGAAACTGA AGAGCCTGGG CCTGGTGGAC 
AGGAACAACG AACCGCTTAC ACACGCCATG TATAACCTGG CCTCTTTGCG TGAGCTGGGT 
GAGACCCAGC GCCGACCATG CACCATCCAG GTGCCCGAGC CCATCCTCCG CAAGATAGAG 
ACCTTCCTGA ACCATTACCC TGTGGAGAGT TCATGGATCG CCCCAGAACT CCGGCTGCAG 
AGTGATGACA TCTTGCCCTT GGGCAAGGAC TCAGGGCCTC TGAGTGACCC TATCACAGGC 
AAGCCCTATG TGCCCCTGTT GGAAGCAGAG GAGGTACGTC TCAGCCAGAG TCTGCTAGAA 
CTGTGGCGGC GGCGAGGGCC GGTCTGGCAG GAGGCCCCCC AGCTACCTGT GGACCCACAT 
CGGGACACCA TCCTCAACGC CATTGAGCAG CACCCGGTGG TGGTCATCTC TGGGGACACG 
GGCTGTGGGA AGACCACGCG CATCCCCCAG CTGTTGCTGG AGCGCTATGT GACCGAGGGC 
CGAGGTGCCC GCTGCAATGT TATCATCACC CAACCTCGCC GCATCTCTGC TGTGTCTGTG 
GCACAGCGGG TCAGCCACGA ACTGGGCCCC TCCCTGCGCC GGAATGTGGG CTTCCAGGTG 
CGGTTGGAAA GTAAGCCCCC ATCCCGAGGC GGGGCCCTGC TCTTCTGCAC TGTGGGTATC 
CTGCTGCGTA AGCTGCAGAG CAACCCCAGC CTGGAGGGCG TGAGCCACGT CATCGTGGAT 
GAGGTGCATG AGCGGGACGT GAACACAGAC TTTCTGCTGA TCCTGCTCAA GGGCCTGCAG 
CGGCTCAACC CGGCCCTGCG GCTGGTGCTC ATGAGTGCCA CAGGGGACAA TGAGCGCTTC 
TCCCGATACT TTGGTGGCTG CCCCGTCATC AAGGTGCCTG GCTTCATGTA CCCAGTCAAG 
GAGCACTACC TAGAGGACAT CCTGGCCAAG TTGGGCAAGC ACCAGTACCT GCACCGGCAC 
CGGCACCATG AGTCTGAGGA TGAATGCGCA CTCGATTTGG ACCTTGTGAC TGATCTGGTT 
CTGCACATCG ATGCTCGCGG GGAACCAGGT GGGATCCTGT GCTTCCTGCC TGGGTGGCAG 
GAGATCAAAG GAGTGCAGCA GCGCCTCCAG GAGGCCCTGG GCATGCACGA GAGCAAGTAC 
CTCATCCTGC CAGTGCACTC CAACATCCCC ATGATGGATC AGAAGGCCAT ATTCCAGCAG 
CCTCCAGTTG GGGTGCGCAA GATTGTCTTG GCCACCAACA TTGCTGAGAC TTCCATCACA 
ATCAATGACA TCGTGCATGT GGTGGACAGT GGGCTGCACA AGGAAGAACG CTATGACCTG 
AAGACCAAGG TGTCCTGCCT GGAGACAGTG TGGGTATCAA GAGCCAATGT GATCCAGCGC 
CGGGGCCGGG CGGGCCGCTG CCAGTCCGGC TTTGCCTACC ACTTGTTCCC TCGAAGCCGG 
CTGGAGAAAA TGGTCCCTTT CCAAGTGCCA GAGATCCTGC GCACACCTCT TGAGAACCTG 
GTGCTGCAAG CGAAAATCCA CATGCCTGAG AAGACGGCGG TGGAGTTCCT GTCCAAGGCT 
GTGGACAGTC CAAACATCAA GGCAGTGGAC GAGGCTGTGA TCTTGCTCCA GGAGATCGGG 
GTGCTGGACC AGCGGGAGTA CCTGACTACC CTGGGGCAGC GCCTGGCTCA CATCTCCACC 
GACCCCCGGT TGGCCAAGGC CATTGTGTTG GCTGCCATCT TCCGTTGCCT GCACCCACTA 
CTGGTGGTCG TTTCCTGCCT CACCCGGGAC CCCTTCAGCA GCAGCCTACA GAACCGGGCA 
GAGGTGGACA AGGTGAAAGC ACTGTTGAGC CATGACAGCG GCAGTGACCA CCTGGCCTTT 
GTGCGGGCTG TCGCCGGCTG GGAGGAGGTG CTGCGTTGGC AGGACCGCAG CTCCCGGGAG 
AATTACCTGG AGGAAAACCT GCTGTACGCA CCCAGCCTGC GCTTCATCCA CGGACTCATC 
AAGCAGTTCT CAGAGAACAT TTATGAGGCC TTCCTGGTGG GGAAGCCCTC GGACTGCACC 
CTGGCCTCCG CCCAGTGCAA CGAGTACAGT GAGGAGGAGG AGCTGGTGAA GGGCGTGCTG 
ATGGCCGGCC TCTACCCCAA CCTCATCCAG GTGAGGCAGG GCAAGGTCAC CCGGCAGGGG 
AAGTTCAAGC CCAACAGCGT CACATATAGG ACCAAATCAG GCAACATCCT GCTGCACAAG 
TCGACCATTA ACAGGGAGGC CACACGGTTA CGGAGCCGAT GGCTGACGTA TTTCATGGCA 
GTCAAGTCCA ATGGCAGCGT CTTCGTCCGG GACTCCTCTC AGGTGCACCC GCTAGCTGTG 
CTGCTGCTGA CCGACGGGGA CGTGCACATC CGTGATGACG GGCGCCGGGC CACCATCTCA 
CTGAGCGACA GTGACCTGCT GCGGCTGGAG GGTGACTCGC GTACCGTGCG GCTGCTGAAG 
GAGCTGCGGC GGGCCCTGGG CCGCATGGTG GAGCGGAGCC TGCGCAGCGA GCTGGCTGCA 
CTTCCCCCCA GCGTACAGGA GGAGCACGGG CAGCTGCTTG CGCTACTGGC AGAGCTGCTG 
CGAGGACCCT GTGGCAGCTT TGATGTGCGC AAGACAGCTG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( NM_138615.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_619520.1 Homo sapiens DExH-box helicase 30 (DHX30), transcript variant 1, mRNA.

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CloneID	OHu09108
Clone ID Related Accession (Same CDS sequence)	NM_138615.2 , XM_011533494.2 , XM_011533494.3 , XM_011533494.1 , NM_138615.3 , XM_011533495.2 , XM_011533495.1
Accession Version	NM_138615.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	putative ATP-dependent RNA helicase DHX30 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC047335.1, AB020697.1 and CD368247.1. On Apr 9, 2009 this sequence version replaced NM_138615.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028405.1, SRR1660807.84452.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 18063578 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGAAAGT CACACTGCAC ATAAAATGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCTGAG CGGCAGGCTG CAGCTGCAGC CTGCCAGCTG 
TTCAAGGGTT GGGGTCTGCT AGGTCCCCGG AATGAGTTGT TTGACGCAGC CAAATACCGA 
GTGCTAGCTG ATCGCTTTGG CTCCCCTGCC GACAGCTGGT GGCGTCCGGA ACCCACCATG 
CCCCCTACTT CCTGGCGGCA GCTGAATCCA GAGAGTATTC GACCAGGGGG ACCTGGGGGC 
CTATCCCGCT CTTTAGGCCG GGAAGAAGAG GAGGACGAGG AGGAAGAGCT AGAAGAAGGG 
ACCATAGATG TTACCGACTT CTTGTCCATG ACCCAGCAGG ATTCCCACGC TCCACTCAGG 
GACTCAAGGG GGAGTTCCTT TGAGATGACA GATGACGACA GTGCCATTAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATTGCAAC GTCATCCTCC 
ACAGCTAAGA ACCTCATGCA GTTCCATACT GTGGGCACCA AGACCAAGCT GTCTACACTC 
ACCCTGCTCT GGCCCTGCCC CATGACCTTT GTTGCCAAAG GGCGCCGCAA AGCAGAGGCT 
GAGAATAAGG CGGCAGCCTT GGCCTGCAAG AAACTGAAGA GCCTGGGCCT GGTGGACAGG 
AACAACGAAC CGCTTACACA CGCCATGTAT AACCTGGCCT CTTTGCGTGA GCTGGGTGAG 
ACCCAGCGCC GACCATGCAC CATCCAGGTG CCCGAGCCCA TCCTCCGCAA GATAGAGACC 
TTCCTGAACC ATTACCCTGT GGAGAGTTCA TGGATCGCCC CAGAACTCCG GCTGCAGAGT 
GATGACATCT TGCCCTTGGG CAAGGACTCA GGGCCTCTGA GTGACCCTAT CACAGGCAAG 
CCCTATGTGC CCCTGTTGGA AGCAGAGGAG GTACGTCTCA GCCAGAGTCT GCTAGAACTG 
TGGCGGCGGC GAGGGCCGGT CTGGCAGGAG GCCCCCCAGC TACCTGTGGA CCCACATCGG 
GACACCATCC TCAACGCCAT TGAGCAGCAC CCGGTGGTGG TCATCTCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG TTGCTGGAGC GCTATGTGAC CGAGGGCCGA 
GGTGCCCGCT GCAATGTTAT CATCACCCAA CCTCGCCGCA TCTCTGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCACGAACT GGGCCCCTCC CTGCGCCGGA ATGTGGGCTT CCAGGTGCGG 
TTGGAAAGTA AGCCCCCATC CCGAGGCGGG GCCCTGCTCT TCTGCACTGT GGGTATCCTG 
CTGCGTAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTCAT CGTGGATGAG 
GTGCATGAGC GGGACGTGAA CACAGACTTT CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CCCTGCGGCT GGTGCTCATG AGTGCCACAG GGGACAATGA GCGCTTCTCC 
CGATACTTTG GTGGCTGCCC CGTCATCAAG GTGCCTGGCT TCATGTACCC AGTCAAGGAG 
CACTACCTAG AGGACATCCT GGCCAAGTTG GGCAAGCACC AGTACCTGCA CCGGCACCGG 
CACCATGAGT CTGAGGATGA ATGCGCACTC GATTTGGACC TTGTGACTGA TCTGGTTCTG 
CACATCGATG CTCGCGGGGA ACCAGGTGGG ATCCTGTGCT TCCTGCCTGG GTGGCAGGAG 
ATCAAAGGAG TGCAGCAGCG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCCATG ATGGATCAGA AGGCCATATT CCAGCAGCCT 
CCAGTTGGGG TGCGCAAGAT TGTCTTGGCC ACCAACATTG CTGAGACTTC CATCACAATC 
AATGACATCG TGCATGTGGT GGACAGTGGG CTGCACAAGG AAGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACAGTGTGG GTATCAAGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCCGGCTTT GCCTACCACT TGTTCCCTCG AAGCCGGCTG 
GAGAAAATGG TCCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCGA AAATCCACAT GCCTGAGAAG ACGGCGGTGG AGTTCCTGTC CAAGGCTGTG 
GACAGTCCAA ACATCAAGGC AGTGGACGAG GCTGTGATCT TGCTCCAGGA GATCGGGGTG 
CTGGACCAGC GGGAGTACCT GACTACCCTG GGGCAGCGCC TGGCTCACAT CTCCACCGAC 
CCCCGGTTGG CCAAGGCCAT TGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTACTG 
GTGGTCGTTT CCTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTACAGAA CCGGGCAGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAT GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCTGTCG CCGGCTGGGA GGAGGTGCTG CGTTGGCAGG ACCGCAGCTC CCGGGAGAAT 
TACCTGGAGG AAAACCTGCT GTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCCTTC CTGGTGGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCCGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG CGTGCTGATG 
GCCGGCCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTCACCCG GCAGGGGAAG 
TTCAAGCCCA ACAGCGTCAC ATATAGGACC AAATCAGGCA ACATCCTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC ACGGTTACGG AGCCGATGGC TGACGTATTT CATGGCAGTC 
AAGTCCAATG GCAGCGTCTT CGTCCGGGAC TCCTCTCAGG TGCACCCGCT AGCTGTGCTG 
CTGCTGACCG ACGGGGACGT GCACATCCGT GATGACGGGC GCCGGGCCAC CATCTCACTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGTA CCGTGCGGCT GCTGAAGGAG 
CTGCGGCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCTGCACTT 
CCCCCCAGCG TACAGGAGGA GCACGGGCAG CTGCTTGCGC TACTGGCAGA GCTGCTGCGA 
GGACCCTGTG GCAGCTTTGA TGTGCGCAAG ACAGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619520.1
CDS	427..4011
Translation	MFSLDSFRKDRAQHRQRQCKLPPPRLPPMCVNPTPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	NM_138615.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138615.2

ORF Insert Sequence:

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGAAAGT CACACTGCAC ATAAAATGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCTGAG CGGCAGGCTG CAGCTGCAGC CTGCCAGCTG 
TTCAAGGGTT GGGGTCTGCT AGGTCCCCGG AATGAGTTGT TTGACGCAGC CAAATACCGA 
GTGCTAGCTG ATCGCTTTGG CTCCCCTGCC GACAGCTGGT GGCGTCCGGA ACCCACCATG 
CCCCCTACTT CCTGGCGGCA GCTGAATCCA GAGAGTATTC GACCAGGGGG ACCTGGGGGC 
CTATCCCGCT CTTTAGGCCG GGAAGAAGAG GAGGACGAGG AGGAAGAGCT AGAAGAAGGG 
ACCATAGATG TTACCGACTT CTTGTCCATG ACCCAGCAGG ATTCCCACGC TCCACTCAGG 
GACTCAAGGG GGAGTTCCTT TGAGATGACA GATGACGACA GTGCCATTAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATTGCAAC GTCATCCTCC 
ACAGCTAAGA ACCTCATGCA GTTCCATACT GTGGGCACCA AGACCAAGCT GTCTACACTC 
ACCCTGCTCT GGCCCTGCCC CATGACCTTT GTTGCCAAAG GGCGCCGCAA AGCAGAGGCT 
GAGAATAAGG CGGCAGCCTT GGCCTGCAAG AAACTGAAGA GCCTGGGCCT GGTGGACAGG 
AACAACGAAC CGCTTACACA CGCCATGTAT AACCTGGCCT CTTTGCGTGA GCTGGGTGAG 
ACCCAGCGCC GACCATGCAC CATCCAGGTG CCCGAGCCCA TCCTCCGCAA GATAGAGACC 
TTCCTGAACC ATTACCCTGT GGAGAGTTCA TGGATCGCCC CAGAACTCCG GCTGCAGAGT 
GATGACATCT TGCCCTTGGG CAAGGACTCA GGGCCTCTGA GTGACCCTAT CACAGGCAAG 
CCCTATGTGC CCCTGTTGGA AGCAGAGGAG GTACGTCTCA GCCAGAGTCT GCTAGAACTG 
TGGCGGCGGC GAGGGCCGGT CTGGCAGGAG GCCCCCCAGC TACCTGTGGA CCCACATCGG 
GACACCATCC TCAACGCCAT TGAGCAGCAC CCGGTGGTGG TCATCTCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG TTGCTGGAGC GCTATGTGAC CGAGGGCCGA 
GGTGCCCGCT GCAATGTTAT CATCACCCAA CCTCGCCGCA TCTCTGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCACGAACT GGGCCCCTCC CTGCGCCGGA ATGTGGGCTT CCAGGTGCGG 
TTGGAAAGTA AGCCCCCATC CCGAGGCGGG GCCCTGCTCT TCTGCACTGT GGGTATCCTG 
CTGCGTAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTCAT CGTGGATGAG 
GTGCATGAGC GGGACGTGAA CACAGACTTT CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CCCTGCGGCT GGTGCTCATG AGTGCCACAG GGGACAATGA GCGCTTCTCC 
CGATACTTTG GTGGCTGCCC CGTCATCAAG GTGCCTGGCT TCATGTACCC AGTCAAGGAG 
CACTACCTAG AGGACATCCT GGCCAAGTTG GGCAAGCACC AGTACCTGCA CCGGCACCGG 
CACCATGAGT CTGAGGATGA ATGCGCACTC GATTTGGACC TTGTGACTGA TCTGGTTCTG 
CACATCGATG CTCGCGGGGA ACCAGGTGGG ATCCTGTGCT TCCTGCCTGG GTGGCAGGAG 
ATCAAAGGAG TGCAGCAGCG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCCATG ATGGATCAGA AGGCCATATT CCAGCAGCCT 
CCAGTTGGGG TGCGCAAGAT TGTCTTGGCC ACCAACATTG CTGAGACTTC CATCACAATC 
AATGACATCG TGCATGTGGT GGACAGTGGG CTGCACAAGG AAGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACAGTGTGG GTATCAAGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCCGGCTTT GCCTACCACT TGTTCCCTCG AAGCCGGCTG 
GAGAAAATGG TCCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCGA AAATCCACAT GCCTGAGAAG ACGGCGGTGG AGTTCCTGTC CAAGGCTGTG 
GACAGTCCAA ACATCAAGGC AGTGGACGAG GCTGTGATCT TGCTCCAGGA GATCGGGGTG 
CTGGACCAGC GGGAGTACCT GACTACCCTG GGGCAGCGCC TGGCTCACAT CTCCACCGAC 
CCCCGGTTGG CCAAGGCCAT TGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTACTG 
GTGGTCGTTT CCTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTACAGAA CCGGGCAGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAT GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCTGTCG CCGGCTGGGA GGAGGTGCTG CGTTGGCAGG ACCGCAGCTC CCGGGAGAAT 
TACCTGGAGG AAAACCTGCT GTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCCTTC CTGGTGGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCCGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG CGTGCTGATG 
GCCGGCCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTCACCCG GCAGGGGAAG 
TTCAAGCCCA ACAGCGTCAC ATATAGGACC AAATCAGGCA ACATCCTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC ACGGTTACGG AGCCGATGGC TGACGTATTT CATGGCAGTC 
AAGTCCAATG GCAGCGTCTT CGTCCGGGAC TCCTCTCAGG TGCACCCGCT AGCTGTGCTG 
CTGCTGACCG ACGGGGACGT GCACATCCGT GATGACGGGC GCCGGGCCAC CATCTCACTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGTA CCGTGCGGCT GCTGAAGGAG 
CTGCGGCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCTGCACTT 
CCCCCCAGCG TACAGGAGGA GCACGGGCAG CTGCTTGCGC TACTGGCAGA GCTGCTGCGA 
GGACCCTGTG GCAGCTTTGA TGTGCGCAAG ACAGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( NM_138615.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_619520.1 Homo sapiens DExH-box helicase 30 (DHX30), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09108
Clone ID Related Accession (Same CDS sequence)	NM_138615.2 , XM_011533494.2 , XM_011533494.3 , XM_011533494.1 , NM_138615.3 , XM_011533495.2 , XM_011533495.1
Accession Version	NM_138615.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX30 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC047335.1, AB020697.1 and CD368247.1. On Nov 23, 2018 this sequence version replaced NM_138615.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028405.1, SRR1660809.198360.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 18063578 MANE Ensembl match :: ENST00000445061.6/ ENSP00000405620.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGAAAGT CACACTGCAC ATAAAATGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCTGAG CGGCAGGCTG CAGCTGCAGC CTGCCAGCTG 
TTCAAGGGTT GGGGTCTGCT AGGTCCCCGG AATGAGTTGT TTGACGCAGC CAAATACCGA 
GTGCTAGCTG ATCGCTTTGG CTCCCCTGCC GACAGCTGGT GGCGTCCGGA ACCCACCATG 
CCCCCTACTT CCTGGCGGCA GCTGAATCCA GAGAGTATTC GACCAGGGGG ACCTGGGGGC 
CTATCCCGCT CTTTAGGCCG GGAAGAAGAG GAGGACGAGG AGGAAGAGCT AGAAGAAGGG 
ACCATAGATG TTACCGACTT CTTGTCCATG ACCCAGCAGG ATTCCCACGC TCCACTCAGG 
GACTCAAGGG GGAGTTCCTT TGAGATGACA GATGACGACA GTGCCATTAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATTGCAAC GTCATCCTCC 
ACAGCTAAGA ACCTCATGCA GTTCCATACT GTGGGCACCA AGACCAAGCT GTCTACACTC 
ACCCTGCTCT GGCCCTGCCC CATGACCTTT GTTGCCAAAG GGCGCCGCAA AGCAGAGGCT 
GAGAATAAGG CGGCAGCCTT GGCCTGCAAG AAACTGAAGA GCCTGGGCCT GGTGGACAGG 
AACAACGAAC CGCTTACACA CGCCATGTAT AACCTGGCCT CTTTGCGTGA GCTGGGTGAG 
ACCCAGCGCC GACCATGCAC CATCCAGGTG CCCGAGCCCA TCCTCCGCAA GATAGAGACC 
TTCCTGAACC ATTACCCTGT GGAGAGTTCA TGGATCGCCC CAGAACTCCG GCTGCAGAGT 
GATGACATCT TGCCCTTGGG CAAGGACTCA GGGCCTCTGA GTGACCCTAT CACAGGCAAG 
CCCTATGTGC CCCTGTTGGA AGCAGAGGAG GTACGTCTCA GCCAGAGTCT GCTAGAACTG 
TGGCGGCGGC GAGGGCCGGT CTGGCAGGAG GCCCCCCAGC TACCTGTGGA CCCACATCGG 
GACACCATCC TCAACGCCAT TGAGCAGCAC CCGGTGGTGG TCATCTCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG TTGCTGGAGC GCTATGTGAC CGAGGGCCGA 
GGTGCCCGCT GCAATGTTAT CATCACCCAA CCTCGCCGCA TCTCTGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCACGAACT GGGCCCCTCC CTGCGCCGGA ATGTGGGCTT CCAGGTGCGG 
TTGGAAAGTA AGCCCCCATC CCGAGGCGGG GCCCTGCTCT TCTGCACTGT GGGTATCCTG 
CTGCGTAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTCAT CGTGGATGAG 
GTGCATGAGC GGGACGTGAA CACAGACTTT CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CCCTGCGGCT GGTGCTCATG AGTGCCACAG GGGACAATGA GCGCTTCTCC 
CGATACTTTG GTGGCTGCCC CGTCATCAAG GTGCCTGGCT TCATGTACCC AGTCAAGGAG 
CACTACCTAG AGGACATCCT GGCCAAGTTG GGCAAGCACC AGTACCTGCA CCGGCACCGG 
CACCATGAGT CTGAGGATGA ATGCGCACTC GATTTGGACC TTGTGACTGA TCTGGTTCTG 
CACATCGATG CTCGCGGGGA ACCAGGTGGG ATCCTGTGCT TCCTGCCTGG GTGGCAGGAG 
ATCAAAGGAG TGCAGCAGCG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCCATG ATGGATCAGA AGGCCATATT CCAGCAGCCT 
CCAGTTGGGG TGCGCAAGAT TGTCTTGGCC ACCAACATTG CTGAGACTTC CATCACAATC 
AATGACATCG TGCATGTGGT GGACAGTGGG CTGCACAAGG AAGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACAGTGTGG GTATCAAGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCCGGCTTT GCCTACCACT TGTTCCCTCG AAGCCGGCTG 
GAGAAAATGG TCCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCGA AAATCCACAT GCCTGAGAAG ACGGCGGTGG AGTTCCTGTC CAAGGCTGTG 
GACAGTCCAA ACATCAAGGC AGTGGACGAG GCTGTGATCT TGCTCCAGGA GATCGGGGTG 
CTGGACCAGC GGGAGTACCT GACTACCCTG GGGCAGCGCC TGGCTCACAT CTCCACCGAC 
CCCCGGTTGG CCAAGGCCAT TGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTACTG 
GTGGTCGTTT CCTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTACAGAA CCGGGCAGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAT GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCTGTCG CCGGCTGGGA GGAGGTGCTG CGTTGGCAGG ACCGCAGCTC CCGGGAGAAT 
TACCTGGAGG AAAACCTGCT GTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCCTTC CTGGTGGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCCGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG CGTGCTGATG 
GCCGGCCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTCACCCG GCAGGGGAAG 
TTCAAGCCCA ACAGCGTCAC ATATAGGACC AAATCAGGCA ACATCCTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC ACGGTTACGG AGCCGATGGC TGACGTATTT CATGGCAGTC 
AAGTCCAATG GCAGCGTCTT CGTCCGGGAC TCCTCTCAGG TGCACCCGCT AGCTGTGCTG 
CTGCTGACCG ACGGGGACGT GCACATCCGT GATGACGGGC GCCGGGCCAC CATCTCACTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGTA CCGTGCGGCT GCTGAAGGAG 
CTGCGGCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCTGCACTT 
CCCCCCAGCG TACAGGAGGA GCACGGGCAG CTGCTTGCGC TACTGGCAGA GCTGCTGCGA 
GGACCCTGTG GCAGCTTTGA TGTGCGCAAG ACAGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619520.1
CDS	198..3782
Translation	MFSLDSFRKDRAQHRQRQCKLPPPRLPPMCVNPTPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	NM_138615.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138615.3

ORF Insert Sequence:

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGAAAGT CACACTGCAC ATAAAATGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCTGAG CGGCAGGCTG CAGCTGCAGC CTGCCAGCTG 
TTCAAGGGTT GGGGTCTGCT AGGTCCCCGG AATGAGTTGT TTGACGCAGC CAAATACCGA 
GTGCTAGCTG ATCGCTTTGG CTCCCCTGCC GACAGCTGGT GGCGTCCGGA ACCCACCATG 
CCCCCTACTT CCTGGCGGCA GCTGAATCCA GAGAGTATTC GACCAGGGGG ACCTGGGGGC 
CTATCCCGCT CTTTAGGCCG GGAAGAAGAG GAGGACGAGG AGGAAGAGCT AGAAGAAGGG 
ACCATAGATG TTACCGACTT CTTGTCCATG ACCCAGCAGG ATTCCCACGC TCCACTCAGG 
GACTCAAGGG GGAGTTCCTT TGAGATGACA GATGACGACA GTGCCATTAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATTGCAAC GTCATCCTCC 
ACAGCTAAGA ACCTCATGCA GTTCCATACT GTGGGCACCA AGACCAAGCT GTCTACACTC 
ACCCTGCTCT GGCCCTGCCC CATGACCTTT GTTGCCAAAG GGCGCCGCAA AGCAGAGGCT 
GAGAATAAGG CGGCAGCCTT GGCCTGCAAG AAACTGAAGA GCCTGGGCCT GGTGGACAGG 
AACAACGAAC CGCTTACACA CGCCATGTAT AACCTGGCCT CTTTGCGTGA GCTGGGTGAG 
ACCCAGCGCC GACCATGCAC CATCCAGGTG CCCGAGCCCA TCCTCCGCAA GATAGAGACC 
TTCCTGAACC ATTACCCTGT GGAGAGTTCA TGGATCGCCC CAGAACTCCG GCTGCAGAGT 
GATGACATCT TGCCCTTGGG CAAGGACTCA GGGCCTCTGA GTGACCCTAT CACAGGCAAG 
CCCTATGTGC CCCTGTTGGA AGCAGAGGAG GTACGTCTCA GCCAGAGTCT GCTAGAACTG 
TGGCGGCGGC GAGGGCCGGT CTGGCAGGAG GCCCCCCAGC TACCTGTGGA CCCACATCGG 
GACACCATCC TCAACGCCAT TGAGCAGCAC CCGGTGGTGG TCATCTCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG TTGCTGGAGC GCTATGTGAC CGAGGGCCGA 
GGTGCCCGCT GCAATGTTAT CATCACCCAA CCTCGCCGCA TCTCTGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCACGAACT GGGCCCCTCC CTGCGCCGGA ATGTGGGCTT CCAGGTGCGG 
TTGGAAAGTA AGCCCCCATC CCGAGGCGGG GCCCTGCTCT TCTGCACTGT GGGTATCCTG 
CTGCGTAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTCAT CGTGGATGAG 
GTGCATGAGC GGGACGTGAA CACAGACTTT CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CCCTGCGGCT GGTGCTCATG AGTGCCACAG GGGACAATGA GCGCTTCTCC 
CGATACTTTG GTGGCTGCCC CGTCATCAAG GTGCCTGGCT TCATGTACCC AGTCAAGGAG 
CACTACCTAG AGGACATCCT GGCCAAGTTG GGCAAGCACC AGTACCTGCA CCGGCACCGG 
CACCATGAGT CTGAGGATGA ATGCGCACTC GATTTGGACC TTGTGACTGA TCTGGTTCTG 
CACATCGATG CTCGCGGGGA ACCAGGTGGG ATCCTGTGCT TCCTGCCTGG GTGGCAGGAG 
ATCAAAGGAG TGCAGCAGCG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCCATG ATGGATCAGA AGGCCATATT CCAGCAGCCT 
CCAGTTGGGG TGCGCAAGAT TGTCTTGGCC ACCAACATTG CTGAGACTTC CATCACAATC 
AATGACATCG TGCATGTGGT GGACAGTGGG CTGCACAAGG AAGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACAGTGTGG GTATCAAGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCCGGCTTT GCCTACCACT TGTTCCCTCG AAGCCGGCTG 
GAGAAAATGG TCCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCGA AAATCCACAT GCCTGAGAAG ACGGCGGTGG AGTTCCTGTC CAAGGCTGTG 
GACAGTCCAA ACATCAAGGC AGTGGACGAG GCTGTGATCT TGCTCCAGGA GATCGGGGTG 
CTGGACCAGC GGGAGTACCT GACTACCCTG GGGCAGCGCC TGGCTCACAT CTCCACCGAC 
CCCCGGTTGG CCAAGGCCAT TGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTACTG 
GTGGTCGTTT CCTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTACAGAA CCGGGCAGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAT GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCTGTCG CCGGCTGGGA GGAGGTGCTG CGTTGGCAGG ACCGCAGCTC CCGGGAGAAT 
TACCTGGAGG AAAACCTGCT GTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCCTTC CTGGTGGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCCGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG CGTGCTGATG 
GCCGGCCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTCACCCG GCAGGGGAAG 
TTCAAGCCCA ACAGCGTCAC ATATAGGACC AAATCAGGCA ACATCCTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC ACGGTTACGG AGCCGATGGC TGACGTATTT CATGGCAGTC 
AAGTCCAATG GCAGCGTCTT CGTCCGGGAC TCCTCTCAGG TGCACCCGCT AGCTGTGCTG 
CTGCTGACCG ACGGGGACGT GCACATCCGT GATGACGGGC GCCGGGCCAC CATCTCACTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGTA CCGTGCGGCT GCTGAAGGAG 
CTGCGGCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCTGCACTT 
CCCCCCAGCG TACAGGAGGA GCACGGGCAG CTGCTTGCGC TACTGGCAGA GCTGCTGCGA 
GGACCCTGTG GCAGCTTTGA TGTGCGCAAG ACAGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( NM_001330990.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317919.1 Homo sapiens DExH-box helicase 30 (DHX30), transcript variant 5, mRNA.

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CloneID	OHu65127
Clone ID Related Accession (Same CDS sequence)	XM_011533497.2 , XM_011533498.1 , XM_011533496.1 , NM_001330990.1
Accession Version	NM_001330990.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX30 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026318.23 and AC139667.5. Transcript Variant: This variant (5) differs in the 5' UTR and 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 4, which is shorter at the N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.211967.1, SRR1660803.64929.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 18063578 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGTGTCA ACCCTACCCC AGGAGGGACC ATCTCTCGAG CTTCTAGGGA CCTATTAAAA 
GAGTTCCCAC AGCCCAAAAA TCTTCTCAAC AGTGTGATTG GAAGAGCCCT CGGCATCTCA 
CATGCAAAAG ACAAACTAGT CTACGTGCAC ACAAATGGAC CGAAGAAAAA GAAAGTCACA 
CTGCACATAA AATGGCCCAA GAGCGTGGAG GTAGAAGGCT ATGGCAGCAA GAAGATCGAT 
GCTGAGCGGC AGGCTGCAGC TGCAGCCTGC CAGCTGTTCA AGGGTTGGGG TCTGCTAGGT 
CCCCGGAATG AGTTGTTTGA CGCAGCCAAA TACCGAGTGC TAGCTGATCG CTTTGGCTCC 
CCTGCCGACA GCTGGTGGCG TCCGGAACCC ACCATGCCCC CTACTTCCTG GCGGCAGCTG 
AATCCAGAGA GTATTCGACC AGGGGGACCT GGGGGCCTAT CCCGCTCTTT AGGCCGGGAA 
GAAGAGGAGG ACGAGGAGGA AGAGCTAGAA GAAGGGACCA TAGATGTTAC CGACTTCTTG 
TCCATGACCC AGCAGGATTC CCACGCTCCA CTCAGGGACT CAAGGGGGAG TTCCTTTGAG 
ATGACAGATG ACGACAGTGC CATTAGGGCT CTGACCCAGT TTCCACTTCC CAAGAACCTT 
CTGGCCAAGG TGATTCAGAT TGCAACGTCA TCCTCCACAG CTAAGAACCT CATGCAGTTC 
CATACTGTGG GCACCAAGAC CAAGCTGTCT ACACTCACCC TGCTCTGGCC CTGCCCCATG 
ACCTTTGTTG CCAAAGGGCG CCGCAAAGCA GAGGCTGAGA ATAAGGCGGC AGCCTTGGCC 
TGCAAGAAAC TGAAGAGCCT GGGCCTGGTG GACAGGAACA ACGAACCGCT TACACACGCC 
ATGTATAACC TGGCCTCTTT GCGTGAGCTG GGTGAGACCC AGCGCCGACC ATGCACCATC 
CAGGTGCCCG AGCCCATCCT CCGCAAGATA GAGACCTTCC TGAACCATTA CCCTGTGGAG 
AGTTCATGGA TCGCCCCAGA ACTCCGGCTG CAGAGTGATG ACATCTTGCC CTTGGGCAAG 
GACTCAGGGC CTCTGAGTGA CCCTATCACA GGCAAGCCCT ATGTGCCCCT GTTGGAAGCA 
GAGGAGGTAC GTCTCAGCCA GAGTCTGCTA GAACTGTGGC GGCGGCGAGG GCCGGTCTGG 
CAGGAGGCCC CCCAGCTACC TGTGGACCCA CATCGGGACA CCATCCTCAA CGCCATTGAG 
CAGCACCCGG TGGTGGTCAT CTCTGGGGAC ACGGGCTGTG GGAAGACCAC GCGCATCCCC 
CAGCTGTTGC TGGAGCGCTA TGTGACCGAG GGCCGAGGTG CCCGCTGCAA TGTTATCATC 
ACCCAACCTC GCCGCATCTC TGCTGTGTCT GTGGCACAGC GGGTCAGCCA CGAACTGGGC 
CCCTCCCTGC GCCGGAATGT GGGCTTCCAG GTGCGGTTGG AAAGTAAGCC CCCATCCCGA 
GGCGGGGCCC TGCTCTTCTG CACTGTGGGT ATCCTGCTGC GTAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GCGTGAGCCA CGTCATCGTG GATGAGGTGC ATGAGCGGGA CGTGAACACA 
GACTTTCTGC TGATCCTGCT CAAGGGCCTG CAGCGGCTCA ACCCGGCCCT GCGGCTGGTG 
CTCATGAGTG CCACAGGGGA CAATGAGCGC TTCTCCCGAT ACTTTGGTGG CTGCCCCGTC 
ATCAAGGTGC CTGGCTTCAT GTACCCAGTC AAGGAGCACT ACCTAGAGGA CATCCTGGCC 
AAGTTGGGCA AGCACCAGTA CCTGCACCGG CACCGGCACC ATGAGTCTGA GGATGAATGC 
GCACTCGATT TGGACCTTGT GACTGATCTG GTTCTGCACA TCGATGCTCG CGGGGAACCA 
GGTGGGATCC TGTGCTTCCT GCCTGGGTGG CAGGAGATCA AAGGAGTGCA GCAGCGCCTC 
CAGGAGGCCC TGGGCATGCA CGAGAGCAAG TACCTCATCC TGCCAGTGCA CTCCAACATC 
CCCATGATGG ATCAGAAGGC CATATTCCAG CAGCCTCCAG TTGGGGTGCG CAAGATTGTC 
TTGGCCACCA ACATTGCTGA GACTTCCATC ACAATCAATG ACATCGTGCA TGTGGTGGAC 
AGTGGGCTGC ACAAGGAAGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACA 
GTGTGGGTAT CAAGAGCCAA TGTGATCCAG CGCCGGGGCC GGGCGGGCCG CTGCCAGTCC 
GGCTTTGCCT ACCACTTGTT CCCTCGAAGC CGGCTGGAGA AAATGGTCCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCGAAAAT CCACATGCCT 
GAGAAGACGG CGGTGGAGTT CCTGTCCAAG GCTGTGGACA GTCCAAACAT CAAGGCAGTG 
GACGAGGCTG TGATCTTGCT CCAGGAGATC GGGGTGCTGG ACCAGCGGGA GTACCTGACT 
ACCCTGGGGC AGCGCCTGGC TCACATCTCC ACCGACCCCC GGTTGGCCAA GGCCATTGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTACTGGTGG TCGTTTCCTG CCTCACCCGG 
GACCCCTTCA GCAGCAGCCT ACAGAACCGG GCAGAGGTGG ACAAGGTGAA AGCACTGTTG 
AGCCATGACA GCGGCAGTGA CCACCTGGCC TTTGTGCGGG CTGTCGCCGG CTGGGAGGAG 
GTGCTGCGTT GGCAGGACCG CAGCTCCCGG GAGAATTACC TGGAGGAAAA CCTGCTGTAC 
GCACCCAGCC TGCGCTTCAT CCACGGACTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCCTTCCTGG TGGGGAAGCC CTCGGACTGC ACCCTGGCCT CCGCCCAGTG CAACGAGTAC 
AGTGAGGAGG AGGAGCTGGT GAAGGGCGTG CTGATGGCCG GCCTCTACCC CAACCTCATC 
CAGGTGAGGC AGGGCAAGGT CACCCGGCAG GGGAAGTTCA AGCCCAACAG CGTCACATAT 
AGGACCAAAT CAGGCAACAT CCTGCTGCAC AAGTCGACCA TTAACAGGGA GGCCACACGG 
TTACGGAGCC GATGGCTGAC GTATTTCATG GCAGTCAAGT CCAATGGCAG CGTCTTCGTC 
CGGGACTCCT CTCAGGTGCA CCCGCTAGCT GTGCTGCTGC TGACCGACGG GGACGTGCAC 
ATCCGTGATG ACGGGCGCCG GGCCACCATC TCACTGAGCG ACAGTGACCT GCTGCGGCTG 
GAGGGTGACT CGCGTACCGT GCGGCTGCTG AAGGAGCTGC GGCGGGCCCT GGGCCGCATG 
GTGGAGCGGA GCCTGCGCAG CGAGCTGGCT GCACTTCCCC CCAGCGTACA GGAGGAGCAC 
GGGCAGCTGC TTGCGCTACT GGCAGAGCTG CTGCGAGGAC CCTGTGGCAG CTTTGATGTG 
CGCAAGACAG CTGACGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317919.1
CDS	677..4177
Translation	MCVNPTPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	NM_001330990.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330990.1

ORF Insert Sequence:

ATGTGTGTCA ACCCTACCCC AGGAGGGACC ATCTCTCGAG CTTCTAGGGA CCTATTAAAA 
GAGTTCCCAC AGCCCAAAAA TCTTCTCAAC AGTGTGATTG GAAGAGCCCT CGGCATCTCA 
CATGCAAAAG ACAAACTAGT CTACGTGCAC ACAAATGGAC CGAAGAAAAA GAAAGTCACA 
CTGCACATAA AATGGCCCAA GAGCGTGGAG GTAGAAGGCT ATGGCAGCAA GAAGATCGAT 
GCTGAGCGGC AGGCTGCAGC TGCAGCCTGC CAGCTGTTCA AGGGTTGGGG TCTGCTAGGT 
CCCCGGAATG AGTTGTTTGA CGCAGCCAAA TACCGAGTGC TAGCTGATCG CTTTGGCTCC 
CCTGCCGACA GCTGGTGGCG TCCGGAACCC ACCATGCCCC CTACTTCCTG GCGGCAGCTG 
AATCCAGAGA GTATTCGACC AGGGGGACCT GGGGGCCTAT CCCGCTCTTT AGGCCGGGAA 
GAAGAGGAGG ACGAGGAGGA AGAGCTAGAA GAAGGGACCA TAGATGTTAC CGACTTCTTG 
TCCATGACCC AGCAGGATTC CCACGCTCCA CTCAGGGACT CAAGGGGGAG TTCCTTTGAG 
ATGACAGATG ACGACAGTGC CATTAGGGCT CTGACCCAGT TTCCACTTCC CAAGAACCTT 
CTGGCCAAGG TGATTCAGAT TGCAACGTCA TCCTCCACAG CTAAGAACCT CATGCAGTTC 
CATACTGTGG GCACCAAGAC CAAGCTGTCT ACACTCACCC TGCTCTGGCC CTGCCCCATG 
ACCTTTGTTG CCAAAGGGCG CCGCAAAGCA GAGGCTGAGA ATAAGGCGGC AGCCTTGGCC 
TGCAAGAAAC TGAAGAGCCT GGGCCTGGTG GACAGGAACA ACGAACCGCT TACACACGCC 
ATGTATAACC TGGCCTCTTT GCGTGAGCTG GGTGAGACCC AGCGCCGACC ATGCACCATC 
CAGGTGCCCG AGCCCATCCT CCGCAAGATA GAGACCTTCC TGAACCATTA CCCTGTGGAG 
AGTTCATGGA TCGCCCCAGA ACTCCGGCTG CAGAGTGATG ACATCTTGCC CTTGGGCAAG 
GACTCAGGGC CTCTGAGTGA CCCTATCACA GGCAAGCCCT ATGTGCCCCT GTTGGAAGCA 
GAGGAGGTAC GTCTCAGCCA GAGTCTGCTA GAACTGTGGC GGCGGCGAGG GCCGGTCTGG 
CAGGAGGCCC CCCAGCTACC TGTGGACCCA CATCGGGACA CCATCCTCAA CGCCATTGAG 
CAGCACCCGG TGGTGGTCAT CTCTGGGGAC ACGGGCTGTG GGAAGACCAC GCGCATCCCC 
CAGCTGTTGC TGGAGCGCTA TGTGACCGAG GGCCGAGGTG CCCGCTGCAA TGTTATCATC 
ACCCAACCTC GCCGCATCTC TGCTGTGTCT GTGGCACAGC GGGTCAGCCA CGAACTGGGC 
CCCTCCCTGC GCCGGAATGT GGGCTTCCAG GTGCGGTTGG AAAGTAAGCC CCCATCCCGA 
GGCGGGGCCC TGCTCTTCTG CACTGTGGGT ATCCTGCTGC GTAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GCGTGAGCCA CGTCATCGTG GATGAGGTGC ATGAGCGGGA CGTGAACACA 
GACTTTCTGC TGATCCTGCT CAAGGGCCTG CAGCGGCTCA ACCCGGCCCT GCGGCTGGTG 
CTCATGAGTG CCACAGGGGA CAATGAGCGC TTCTCCCGAT ACTTTGGTGG CTGCCCCGTC 
ATCAAGGTGC CTGGCTTCAT GTACCCAGTC AAGGAGCACT ACCTAGAGGA CATCCTGGCC 
AAGTTGGGCA AGCACCAGTA CCTGCACCGG CACCGGCACC ATGAGTCTGA GGATGAATGC 
GCACTCGATT TGGACCTTGT GACTGATCTG GTTCTGCACA TCGATGCTCG CGGGGAACCA 
GGTGGGATCC TGTGCTTCCT GCCTGGGTGG CAGGAGATCA AAGGAGTGCA GCAGCGCCTC 
CAGGAGGCCC TGGGCATGCA CGAGAGCAAG TACCTCATCC TGCCAGTGCA CTCCAACATC 
CCCATGATGG ATCAGAAGGC CATATTCCAG CAGCCTCCAG TTGGGGTGCG CAAGATTGTC 
TTGGCCACCA ACATTGCTGA GACTTCCATC ACAATCAATG ACATCGTGCA TGTGGTGGAC 
AGTGGGCTGC ACAAGGAAGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACA 
GTGTGGGTAT CAAGAGCCAA TGTGATCCAG CGCCGGGGCC GGGCGGGCCG CTGCCAGTCC 
GGCTTTGCCT ACCACTTGTT CCCTCGAAGC CGGCTGGAGA AAATGGTCCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCGAAAAT CCACATGCCT 
GAGAAGACGG CGGTGGAGTT CCTGTCCAAG GCTGTGGACA GTCCAAACAT CAAGGCAGTG 
GACGAGGCTG TGATCTTGCT CCAGGAGATC GGGGTGCTGG ACCAGCGGGA GTACCTGACT 
ACCCTGGGGC AGCGCCTGGC TCACATCTCC ACCGACCCCC GGTTGGCCAA GGCCATTGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTACTGGTGG TCGTTTCCTG CCTCACCCGG 
GACCCCTTCA GCAGCAGCCT ACAGAACCGG GCAGAGGTGG ACAAGGTGAA AGCACTGTTG 
AGCCATGACA GCGGCAGTGA CCACCTGGCC TTTGTGCGGG CTGTCGCCGG CTGGGAGGAG 
GTGCTGCGTT GGCAGGACCG CAGCTCCCGG GAGAATTACC TGGAGGAAAA CCTGCTGTAC 
GCACCCAGCC TGCGCTTCAT CCACGGACTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCCTTCCTGG TGGGGAAGCC CTCGGACTGC ACCCTGGCCT CCGCCCAGTG CAACGAGTAC 
AGTGAGGAGG AGGAGCTGGT GAAGGGCGTG CTGATGGCCG GCCTCTACCC CAACCTCATC 
CAGGTGAGGC AGGGCAAGGT CACCCGGCAG GGGAAGTTCA AGCCCAACAG CGTCACATAT 
AGGACCAAAT CAGGCAACAT CCTGCTGCAC AAGTCGACCA TTAACAGGGA GGCCACACGG 
TTACGGAGCC GATGGCTGAC GTATTTCATG GCAGTCAAGT CCAATGGCAG CGTCTTCGTC 
CGGGACTCCT CTCAGGTGCA CCCGCTAGCT GTGCTGCTGC TGACCGACGG GGACGTGCAC 
ATCCGTGATG ACGGGCGCCG GGCCACCATC TCACTGAGCG ACAGTGACCT GCTGCGGCTG 
GAGGGTGACT CGCGTACCGT GCGGCTGCTG AAGGAGCTGC GGCGGGCCCT GGGCCGCATG 
GTGGAGCGGA GCCTGCGCAG CGAGCTGGCT GCACTTCCCC CCAGCGTACA GGAGGAGCAC 
GGGCAGCTGC TTGCGCTACT GGCAGAGCTG CTGCGAGGAC CCTGTGGCAG CTTTGATGTG 
CGCAAGACAG CTGACGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_017005917.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861406.1 Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X11, mRNA.

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CloneID	OHu09083
Clone ID Related Accession (Same CDS sequence)	NM_014966.3 , XM_017005917.1
Accession Version	XM_017005917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX30 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTT CTAGGGACCT ATTAAAAGAG TTCCCACAGC CCAAAAATCT TCTCAACAGT 
GTGATTGGAA GAGCCCTCGG CATCTCACAT GCAAAAGACA AACTAGTCTA CGTGCACACA 
AATGGACCGA AGAAAAAGAA AGTCACACTG CACATAAAAT GGCCCAAGAG CGTGGAGGTA 
GAAGGCTATG GCAGCAAGAA GATCGATGCT GAGCGGCAGG CTGCAGCTGC AGCCTGCCAG 
CTGTTCAAGG GTTGGGGTCT GCTAGGTCCC CGGAATGAGT TGTTTGACGC AGCCAAATAC 
CGAGTGCTAG CTGATCGCTT TGGCTCCCCT GCCGACAGCT GGTGGCGTCC GGAACCCACC 
ATGCCCCCTA CTTCCTGGCG GCAGCTGAAT CCAGAGAGTA TTCGACCAGG GGGACCTGGG 
GGCCTATCCC GCTCTTTAGG CCGGGAAGAA GAGGAGGACG AGGAGGAAGA GCTAGAAGAA 
GGGACCATAG ATGTTACCGA CTTCTTGTCC ATGACCCAGC AGGATTCCCA CGCTCCACTC 
AGGGACTCAA GGGGGAGTTC CTTTGAGATG ACAGATGACG ACAGTGCCAT TAGGGCTCTG 
ACCCAGTTTC CACTTCCCAA GAACCTTCTG GCCAAGGTGA TTCAGATTGC AACGTCATCC 
TCCACAGCTA AGAACCTCAT GCAGTTCCAT ACTGTGGGCA CCAAGACCAA GCTGTCTACA 
CTCACCCTGC TCTGGCCCTG CCCCATGACC TTTGTTGCCA AAGGGCGCCG CAAAGCAGAG 
GCTGAGAATA AGGCGGCAGC CTTGGCCTGC AAGAAACTGA AGAGCCTGGG CCTGGTGGAC 
AGGAACAACG AACCGCTTAC ACACGCCATG TATAACCTGG CCTCTTTGCG TGAGCTGGGT 
GAGACCCAGC GCCGACCATG CACCATCCAG GTGCCCGAGC CCATCCTCCG CAAGATAGAG 
ACCTTCCTGA ACCATTACCC TGTGGAGAGT TCATGGATCG CCCCAGAACT CCGGCTGCAG 
AGTGATGACA TCTTGCCCTT GGGCAAGGAC TCAGGGCCTC TGAGTGACCC TATCACAGGC 
AAGCCCTATG TGCCCCTGTT GGAAGCAGAG GAGGTACGTC TCAGCCAGAG TCTGCTAGAA 
CTGTGGCGGC GGCGAGGGCC GGTCTGGCAG GAGGCCCCCC AGCTACCTGT GGACCCACAT 
CGGGACACCA TCCTCAACGC CATTGAGCAG CACCCGGTGG TGGTCATCTC TGGGGACACG 
GGCTGTGGGA AGACCACGCG CATCCCCCAG CTGTTGCTGG AGCGCTATGT GACCGAGGGC 
CGAGGTGCCC GCTGCAATGT TATCATCACC CAACCTCGCC GCATCTCTGC TGTGTCTGTG 
GCACAGCGGG TCAGCCACGA ACTGGGCCCC TCCCTGCGCC GGAATGTGGG CTTCCAGGTG 
CGGTTGGAAA GTAAGCCCCC ATCCCGAGGC GGGGCCCTGC TCTTCTGCAC TGTGGGTATC 
CTGCTGCGTA AGCTGCAGAG CAACCCCAGC CTGGAGGGCG TGAGCCACGT CATCGTGGAT 
GAGGTGCATG AGCGGGACGT GAACACAGAC TTTCTGCTGA TCCTGCTCAA GGGCCTGCAG 
CGGCTCAACC CGGCCCTGCG GCTGGTGCTC ATGAGTGCCA CAGGGGACAA TGAGCGCTTC 
TCCCGATACT TTGGTGGCTG CCCCGTCATC AAGGTGCCTG GCTTCATGTA CCCAGTCAAG 
GAGCACTACC TAGAGGACAT CCTGGCCAAG TTGGGCAAGC ACCAGTACCT GCACCGGCAC 
CGGCACCATG AGTCTGAGGA TGAATGCGCA CTCGATTTGG ACCTTGTGAC TGATCTGGTT 
CTGCACATCG ATGCTCGCGG GGAACCAGGT GGGATCCTGT GCTTCCTGCC TGGGTGGCAG 
GAGATCAAAG GAGTGCAGCA GCGCCTCCAG GAGGCCCTGG GCATGCACGA GAGCAAGTAC 
CTCATCCTGC CAGTGCACTC CAACATCCCC ATGATGGATC AGAAGGCCAT ATTCCAGCAG 
CCTCCAGTTG GGGTGCGCAA GATTGTCTTG GCCACCAACA TTGCTGAGAC TTCCATCACA 
ATCAATGACA TCGTGCATGT GGTGGACAGT GGGCTGCACA AGGAAGAACG CTATGACCTG 
AAGACCAAGG TGTCCTGCCT GGAGACAGTG TGGGTATCAA GAGCCAATGT GATCCAGCGC 
CGGGGCCGGG CGGGCCGCTG CCAGTCCGGC TTTGCCTACC ACTTGTTCCC TCGAAGCCGG 
CTGGAGAAAA TGGTCCCTTT CCAAGTGCCA GAGATCCTGC GCACACCTCT TGAGAACCTG 
GTGCTGCAAG CGAAAATCCA CATGCCTGAG AAGACGGCGG TGGAGTTCCT GTCCAAGGCT 
GTGGACAGTC CAAACATCAA GGCAGTGGAC GAGGCTGTGA TCTTGCTCCA GGAGATCGGG 
GTGCTGGACC AGCGGGAGTA CCTGACTACC CTGGGGCAGC GCCTGGCTCA CATCTCCACC 
GACCCCCGGT TGGCCAAGGC CATTGTGTTG GCTGCCATCT TCCGTTGCCT GCACCCACTA 
CTGGTGGTCG TTTCCTGCCT CACCCGGGAC CCCTTCAGCA GCAGCCTACA GAACCGGGCA 
GAGGTGGACA AGGTGAAAGC ACTGTTGAGC CATGACAGCG GCAGTGACCA CCTGGCCTTT 
GTGCGGGCTG TCGCCGGCTG GGAGGAGGTG CTGCGTTGGC AGGACCGCAG CTCCCGGGAG 
AATTACCTGG AGGAAAACCT GCTGTACGCA CCCAGCCTGC GCTTCATCCA CGGACTCATC 
AAGCAGTTCT CAGAGAACAT TTATGAGGCC TTCCTGGTGG GGAAGCCCTC GGACTGCACC 
CTGGCCTCCG CCCAGTGCAA CGAGTACAGT GAGGAGGAGG AGCTGGTGAA GGGCGTGCTG 
ATGGCCGGCC TCTACCCCAA CCTCATCCAG GTGAGGCAGG GCAAGGTCAC CCGGCAGGGG 
AAGTTCAAGC CCAACAGCGT CACATATAGG ACCAAATCAG GCAACATCCT GCTGCACAAG 
TCGACCATTA ACAGGGAGGC CACACGGTTA CGGAGCCGAT GGCTGACGTA TTTCATGGCA 
GTCAAGTCCA ATGGCAGCGT CTTCGTCCGG GACTCCTCTC AGGTGCACCC GCTAGCTGTG 
CTGCTGCTGA CCGACGGGGA CGTGCACATC CGTGATGACG GGCGCCGGGC CACCATCTCA 
CTGAGCGACA GTGACCTGCT GCGGCTGGAG GGTGACTCGC GTACCGTGCG GCTGCTGAAG 
GAGCTGCGGC GGGCCCTGGG CCGCATGGTG GAGCGGAGCC TGCGCAGCGA GCTGGCTGCA 
CTTCCCCCCA GCGTACAGGA GGAGCACGGG CAGCTGCTTG CGCTACTGGC AGAGCTGCTG 
CGAGGACCCT GTGGCAGCTT TGATGTGCGC AAGACAGCTG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861406.1
CDS	615..4082
Translation	MAASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_017005917.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005917.1

ORF Insert Sequence:

ATGGCAGCTT CTAGGGACCT ATTAAAAGAG TTCCCACAGC CCAAAAATCT TCTCAACAGT 
GTGATTGGAA GAGCCCTCGG CATCTCACAT GCAAAAGACA AACTAGTCTA CGTGCACACA 
AATGGACCGA AGAAAAAGAA AGTCACACTG CACATAAAAT GGCCCAAGAG CGTGGAGGTA 
GAAGGCTATG GCAGCAAGAA GATCGATGCT GAGCGGCAGG CTGCAGCTGC AGCCTGCCAG 
CTGTTCAAGG GTTGGGGTCT GCTAGGTCCC CGGAATGAGT TGTTTGACGC AGCCAAATAC 
CGAGTGCTAG CTGATCGCTT TGGCTCCCCT GCCGACAGCT GGTGGCGTCC GGAACCCACC 
ATGCCCCCTA CTTCCTGGCG GCAGCTGAAT CCAGAGAGTA TTCGACCAGG GGGACCTGGG 
GGCCTATCCC GCTCTTTAGG CCGGGAAGAA GAGGAGGACG AGGAGGAAGA GCTAGAAGAA 
GGGACCATAG ATGTTACCGA CTTCTTGTCC ATGACCCAGC AGGATTCCCA CGCTCCACTC 
AGGGACTCAA GGGGGAGTTC CTTTGAGATG ACAGATGACG ACAGTGCCAT TAGGGCTCTG 
ACCCAGTTTC CACTTCCCAA GAACCTTCTG GCCAAGGTGA TTCAGATTGC AACGTCATCC 
TCCACAGCTA AGAACCTCAT GCAGTTCCAT ACTGTGGGCA CCAAGACCAA GCTGTCTACA 
CTCACCCTGC TCTGGCCCTG CCCCATGACC TTTGTTGCCA AAGGGCGCCG CAAAGCAGAG 
GCTGAGAATA AGGCGGCAGC CTTGGCCTGC AAGAAACTGA AGAGCCTGGG CCTGGTGGAC 
AGGAACAACG AACCGCTTAC ACACGCCATG TATAACCTGG CCTCTTTGCG TGAGCTGGGT 
GAGACCCAGC GCCGACCATG CACCATCCAG GTGCCCGAGC CCATCCTCCG CAAGATAGAG 
ACCTTCCTGA ACCATTACCC TGTGGAGAGT TCATGGATCG CCCCAGAACT CCGGCTGCAG 
AGTGATGACA TCTTGCCCTT GGGCAAGGAC TCAGGGCCTC TGAGTGACCC TATCACAGGC 
AAGCCCTATG TGCCCCTGTT GGAAGCAGAG GAGGTACGTC TCAGCCAGAG TCTGCTAGAA 
CTGTGGCGGC GGCGAGGGCC GGTCTGGCAG GAGGCCCCCC AGCTACCTGT GGACCCACAT 
CGGGACACCA TCCTCAACGC CATTGAGCAG CACCCGGTGG TGGTCATCTC TGGGGACACG 
GGCTGTGGGA AGACCACGCG CATCCCCCAG CTGTTGCTGG AGCGCTATGT GACCGAGGGC 
CGAGGTGCCC GCTGCAATGT TATCATCACC CAACCTCGCC GCATCTCTGC TGTGTCTGTG 
GCACAGCGGG TCAGCCACGA ACTGGGCCCC TCCCTGCGCC GGAATGTGGG CTTCCAGGTG 
CGGTTGGAAA GTAAGCCCCC ATCCCGAGGC GGGGCCCTGC TCTTCTGCAC TGTGGGTATC 
CTGCTGCGTA AGCTGCAGAG CAACCCCAGC CTGGAGGGCG TGAGCCACGT CATCGTGGAT 
GAGGTGCATG AGCGGGACGT GAACACAGAC TTTCTGCTGA TCCTGCTCAA GGGCCTGCAG 
CGGCTCAACC CGGCCCTGCG GCTGGTGCTC ATGAGTGCCA CAGGGGACAA TGAGCGCTTC 
TCCCGATACT TTGGTGGCTG CCCCGTCATC AAGGTGCCTG GCTTCATGTA CCCAGTCAAG 
GAGCACTACC TAGAGGACAT CCTGGCCAAG TTGGGCAAGC ACCAGTACCT GCACCGGCAC 
CGGCACCATG AGTCTGAGGA TGAATGCGCA CTCGATTTGG ACCTTGTGAC TGATCTGGTT 
CTGCACATCG ATGCTCGCGG GGAACCAGGT GGGATCCTGT GCTTCCTGCC TGGGTGGCAG 
GAGATCAAAG GAGTGCAGCA GCGCCTCCAG GAGGCCCTGG GCATGCACGA GAGCAAGTAC 
CTCATCCTGC CAGTGCACTC CAACATCCCC ATGATGGATC AGAAGGCCAT ATTCCAGCAG 
CCTCCAGTTG GGGTGCGCAA GATTGTCTTG GCCACCAACA TTGCTGAGAC TTCCATCACA 
ATCAATGACA TCGTGCATGT GGTGGACAGT GGGCTGCACA AGGAAGAACG CTATGACCTG 
AAGACCAAGG TGTCCTGCCT GGAGACAGTG TGGGTATCAA GAGCCAATGT GATCCAGCGC 
CGGGGCCGGG CGGGCCGCTG CCAGTCCGGC TTTGCCTACC ACTTGTTCCC TCGAAGCCGG 
CTGGAGAAAA TGGTCCCTTT CCAAGTGCCA GAGATCCTGC GCACACCTCT TGAGAACCTG 
GTGCTGCAAG CGAAAATCCA CATGCCTGAG AAGACGGCGG TGGAGTTCCT GTCCAAGGCT 
GTGGACAGTC CAAACATCAA GGCAGTGGAC GAGGCTGTGA TCTTGCTCCA GGAGATCGGG 
GTGCTGGACC AGCGGGAGTA CCTGACTACC CTGGGGCAGC GCCTGGCTCA CATCTCCACC 
GACCCCCGGT TGGCCAAGGC CATTGTGTTG GCTGCCATCT TCCGTTGCCT GCACCCACTA 
CTGGTGGTCG TTTCCTGCCT CACCCGGGAC CCCTTCAGCA GCAGCCTACA GAACCGGGCA 
GAGGTGGACA AGGTGAAAGC ACTGTTGAGC CATGACAGCG GCAGTGACCA CCTGGCCTTT 
GTGCGGGCTG TCGCCGGCTG GGAGGAGGTG CTGCGTTGGC AGGACCGCAG CTCCCGGGAG 
AATTACCTGG AGGAAAACCT GCTGTACGCA CCCAGCCTGC GCTTCATCCA CGGACTCATC 
AAGCAGTTCT CAGAGAACAT TTATGAGGCC TTCCTGGTGG GGAAGCCCTC GGACTGCACC 
CTGGCCTCCG CCCAGTGCAA CGAGTACAGT GAGGAGGAGG AGCTGGTGAA GGGCGTGCTG 
ATGGCCGGCC TCTACCCCAA CCTCATCCAG GTGAGGCAGG GCAAGGTCAC CCGGCAGGGG 
AAGTTCAAGC CCAACAGCGT CACATATAGG ACCAAATCAG GCAACATCCT GCTGCACAAG 
TCGACCATTA ACAGGGAGGC CACACGGTTA CGGAGCCGAT GGCTGACGTA TTTCATGGCA 
GTCAAGTCCA ATGGCAGCGT CTTCGTCCGG GACTCCTCTC AGGTGCACCC GCTAGCTGTG 
CTGCTGCTGA CCGACGGGGA CGTGCACATC CGTGATGACG GGCGCCGGGC CACCATCTCA 
CTGAGCGACA GTGACCTGCT GCGGCTGGAG GGTGACTCGC GTACCGTGCG GCTGCTGAAG 
GAGCTGCGGC GGGCCCTGGG CCGCATGGTG GAGCGGAGCC TGCGCAGCGA GCTGGCTGCA 
CTTCCCCCCA GCGTACAGGA GGAGCACGGG CAGCTGCTTG CGCTACTGGC AGAGCTGCTG 
CGAGGACCCT GTGGCAGCTT TGATGTGCGC AAGACAGCTG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_011533494.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531796.1 Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X6, mRNA.

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CloneID	OHu09108
Clone ID Related Accession (Same CDS sequence)	NM_138615.2 , XM_011533494.2 , XM_011533494.3 , XM_011533494.1 , NM_138615.3 , XM_011533495.2 , XM_011533495.1
Accession Version	XM_011533494.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative ATP-dependent RNA helicase DHX30 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533494.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGAAAGT CACACTGCAC ATAAAATGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCTGAG CGGCAGGCTG CAGCTGCAGC CTGCCAGCTG 
TTCAAGGGTT GGGGTCTGCT AGGTCCCCGG AATGAGTTGT TTGACGCAGC CAAATACCGA 
GTGCTAGCTG ATCGCTTTGG CTCCCCTGCC GACAGCTGGT GGCGTCCGGA ACCCACCATG 
CCCCCTACTT CCTGGCGGCA GCTGAATCCA GAGAGTATTC GACCAGGGGG ACCTGGGGGC 
CTATCCCGCT CTTTAGGCCG GGAAGAAGAG GAGGACGAGG AGGAAGAGCT AGAAGAAGGG 
ACCATAGATG TTACCGACTT CTTGTCCATG ACCCAGCAGG ATTCCCACGC TCCACTCAGG 
GACTCAAGGG GGAGTTCCTT TGAGATGACA GATGACGACA GTGCCATTAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATTGCAAC GTCATCCTCC 
ACAGCTAAGA ACCTCATGCA GTTCCATACT GTGGGCACCA AGACCAAGCT GTCTACACTC 
ACCCTGCTCT GGCCCTGCCC CATGACCTTT GTTGCCAAAG GGCGCCGCAA AGCAGAGGCT 
GAGAATAAGG CGGCAGCCTT GGCCTGCAAG AAACTGAAGA GCCTGGGCCT GGTGGACAGG 
AACAACGAAC CGCTTACACA CGCCATGTAT AACCTGGCCT CTTTGCGTGA GCTGGGTGAG 
ACCCAGCGCC GACCATGCAC CATCCAGGTG CCCGAGCCCA TCCTCCGCAA GATAGAGACC 
TTCCTGAACC ATTACCCTGT GGAGAGTTCA TGGATCGCCC CAGAACTCCG GCTGCAGAGT 
GATGACATCT TGCCCTTGGG CAAGGACTCA GGGCCTCTGA GTGACCCTAT CACAGGCAAG 
CCCTATGTGC CCCTGTTGGA AGCAGAGGAG GTACGTCTCA GCCAGAGTCT GCTAGAACTG 
TGGCGGCGGC GAGGGCCGGT CTGGCAGGAG GCCCCCCAGC TACCTGTGGA CCCACATCGG 
GACACCATCC TCAACGCCAT TGAGCAGCAC CCGGTGGTGG TCATCTCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG TTGCTGGAGC GCTATGTGAC CGAGGGCCGA 
GGTGCCCGCT GCAATGTTAT CATCACCCAA CCTCGCCGCA TCTCTGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCACGAACT GGGCCCCTCC CTGCGCCGGA ATGTGGGCTT CCAGGTGCGG 
TTGGAAAGTA AGCCCCCATC CCGAGGCGGG GCCCTGCTCT TCTGCACTGT GGGTATCCTG 
CTGCGTAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTCAT CGTGGATGAG 
GTGCATGAGC GGGACGTGAA CACAGACTTT CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CCCTGCGGCT GGTGCTCATG AGTGCCACAG GGGACAATGA GCGCTTCTCC 
CGATACTTTG GTGGCTGCCC CGTCATCAAG GTGCCTGGCT TCATGTACCC AGTCAAGGAG 
CACTACCTAG AGGACATCCT GGCCAAGTTG GGCAAGCACC AGTACCTGCA CCGGCACCGG 
CACCATGAGT CTGAGGATGA ATGCGCACTC GATTTGGACC TTGTGACTGA TCTGGTTCTG 
CACATCGATG CTCGCGGGGA ACCAGGTGGG ATCCTGTGCT TCCTGCCTGG GTGGCAGGAG 
ATCAAAGGAG TGCAGCAGCG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCCATG ATGGATCAGA AGGCCATATT CCAGCAGCCT 
CCAGTTGGGG TGCGCAAGAT TGTCTTGGCC ACCAACATTG CTGAGACTTC CATCACAATC 
AATGACATCG TGCATGTGGT GGACAGTGGG CTGCACAAGG AAGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACAGTGTGG GTATCAAGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCCGGCTTT GCCTACCACT TGTTCCCTCG AAGCCGGCTG 
GAGAAAATGG TCCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCGA AAATCCACAT GCCTGAGAAG ACGGCGGTGG AGTTCCTGTC CAAGGCTGTG 
GACAGTCCAA ACATCAAGGC AGTGGACGAG GCTGTGATCT TGCTCCAGGA GATCGGGGTG 
CTGGACCAGC GGGAGTACCT GACTACCCTG GGGCAGCGCC TGGCTCACAT CTCCACCGAC 
CCCCGGTTGG CCAAGGCCAT TGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTACTG 
GTGGTCGTTT CCTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTACAGAA CCGGGCAGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAT GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCTGTCG CCGGCTGGGA GGAGGTGCTG CGTTGGCAGG ACCGCAGCTC CCGGGAGAAT 
TACCTGGAGG AAAACCTGCT GTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCCTTC CTGGTGGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCCGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG CGTGCTGATG 
GCCGGCCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTCACCCG GCAGGGGAAG 
TTCAAGCCCA ACAGCGTCAC ATATAGGACC AAATCAGGCA ACATCCTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC ACGGTTACGG AGCCGATGGC TGACGTATTT CATGGCAGTC 
AAGTCCAATG GCAGCGTCTT CGTCCGGGAC TCCTCTCAGG TGCACCCGCT AGCTGTGCTG 
CTGCTGACCG ACGGGGACGT GCACATCCGT GATGACGGGC GCCGGGCCAC CATCTCACTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGTA CCGTGCGGCT GCTGAAGGAG 
CTGCGGCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCTGCACTT 
CCCCCCAGCG TACAGGAGGA GCACGGGCAG CTGCTTGCGC TACTGGCAGA GCTGCTGCGA 
GGACCCTGTG GCAGCTTTGA TGTGCGCAAG ACAGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531796.1
CDS	391..3975
Translation	MFSLDSFRKDRAQHRQRQCKLPPPRLPPMCVNPTPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_011533494.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533494.2

ORF Insert Sequence:

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGAAAGT CACACTGCAC ATAAAATGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCTGAG CGGCAGGCTG CAGCTGCAGC CTGCCAGCTG 
TTCAAGGGTT GGGGTCTGCT AGGTCCCCGG AATGAGTTGT TTGACGCAGC CAAATACCGA 
GTGCTAGCTG ATCGCTTTGG CTCCCCTGCC GACAGCTGGT GGCGTCCGGA ACCCACCATG 
CCCCCTACTT CCTGGCGGCA GCTGAATCCA GAGAGTATTC GACCAGGGGG ACCTGGGGGC 
CTATCCCGCT CTTTAGGCCG GGAAGAAGAG GAGGACGAGG AGGAAGAGCT AGAAGAAGGG 
ACCATAGATG TTACCGACTT CTTGTCCATG ACCCAGCAGG ATTCCCACGC TCCACTCAGG 
GACTCAAGGG GGAGTTCCTT TGAGATGACA GATGACGACA GTGCCATTAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATTGCAAC GTCATCCTCC 
ACAGCTAAGA ACCTCATGCA GTTCCATACT GTGGGCACCA AGACCAAGCT GTCTACACTC 
ACCCTGCTCT GGCCCTGCCC CATGACCTTT GTTGCCAAAG GGCGCCGCAA AGCAGAGGCT 
GAGAATAAGG CGGCAGCCTT GGCCTGCAAG AAACTGAAGA GCCTGGGCCT GGTGGACAGG 
AACAACGAAC CGCTTACACA CGCCATGTAT AACCTGGCCT CTTTGCGTGA GCTGGGTGAG 
ACCCAGCGCC GACCATGCAC CATCCAGGTG CCCGAGCCCA TCCTCCGCAA GATAGAGACC 
TTCCTGAACC ATTACCCTGT GGAGAGTTCA TGGATCGCCC CAGAACTCCG GCTGCAGAGT 
GATGACATCT TGCCCTTGGG CAAGGACTCA GGGCCTCTGA GTGACCCTAT CACAGGCAAG 
CCCTATGTGC CCCTGTTGGA AGCAGAGGAG GTACGTCTCA GCCAGAGTCT GCTAGAACTG 
TGGCGGCGGC GAGGGCCGGT CTGGCAGGAG GCCCCCCAGC TACCTGTGGA CCCACATCGG 
GACACCATCC TCAACGCCAT TGAGCAGCAC CCGGTGGTGG TCATCTCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG TTGCTGGAGC GCTATGTGAC CGAGGGCCGA 
GGTGCCCGCT GCAATGTTAT CATCACCCAA CCTCGCCGCA TCTCTGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCACGAACT GGGCCCCTCC CTGCGCCGGA ATGTGGGCTT CCAGGTGCGG 
TTGGAAAGTA AGCCCCCATC CCGAGGCGGG GCCCTGCTCT TCTGCACTGT GGGTATCCTG 
CTGCGTAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTCAT CGTGGATGAG 
GTGCATGAGC GGGACGTGAA CACAGACTTT CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CCCTGCGGCT GGTGCTCATG AGTGCCACAG GGGACAATGA GCGCTTCTCC 
CGATACTTTG GTGGCTGCCC CGTCATCAAG GTGCCTGGCT TCATGTACCC AGTCAAGGAG 
CACTACCTAG AGGACATCCT GGCCAAGTTG GGCAAGCACC AGTACCTGCA CCGGCACCGG 
CACCATGAGT CTGAGGATGA ATGCGCACTC GATTTGGACC TTGTGACTGA TCTGGTTCTG 
CACATCGATG CTCGCGGGGA ACCAGGTGGG ATCCTGTGCT TCCTGCCTGG GTGGCAGGAG 
ATCAAAGGAG TGCAGCAGCG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCCATG ATGGATCAGA AGGCCATATT CCAGCAGCCT 
CCAGTTGGGG TGCGCAAGAT TGTCTTGGCC ACCAACATTG CTGAGACTTC CATCACAATC 
AATGACATCG TGCATGTGGT GGACAGTGGG CTGCACAAGG AAGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACAGTGTGG GTATCAAGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCCGGCTTT GCCTACCACT TGTTCCCTCG AAGCCGGCTG 
GAGAAAATGG TCCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCGA AAATCCACAT GCCTGAGAAG ACGGCGGTGG AGTTCCTGTC CAAGGCTGTG 
GACAGTCCAA ACATCAAGGC AGTGGACGAG GCTGTGATCT TGCTCCAGGA GATCGGGGTG 
CTGGACCAGC GGGAGTACCT GACTACCCTG GGGCAGCGCC TGGCTCACAT CTCCACCGAC 
CCCCGGTTGG CCAAGGCCAT TGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTACTG 
GTGGTCGTTT CCTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTACAGAA CCGGGCAGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAT GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCTGTCG CCGGCTGGGA GGAGGTGCTG CGTTGGCAGG ACCGCAGCTC CCGGGAGAAT 
TACCTGGAGG AAAACCTGCT GTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCCTTC CTGGTGGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCCGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG CGTGCTGATG 
GCCGGCCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTCACCCG GCAGGGGAAG 
TTCAAGCCCA ACAGCGTCAC ATATAGGACC AAATCAGGCA ACATCCTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC ACGGTTACGG AGCCGATGGC TGACGTATTT CATGGCAGTC 
AAGTCCAATG GCAGCGTCTT CGTCCGGGAC TCCTCTCAGG TGCACCCGCT AGCTGTGCTG 
CTGCTGACCG ACGGGGACGT GCACATCCGT GATGACGGGC GCCGGGCCAC CATCTCACTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGTA CCGTGCGGCT GCTGAAGGAG 
CTGCGGCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCTGCACTT 
CCCCCCAGCG TACAGGAGGA GCACGGGCAG CTGCTTGCGC TACTGGCAGA GCTGCTGCGA 
GGACCCTGTG GCAGCTTTGA TGTGCGCAAG ACAGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_011533494.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531796.1 Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X4, mRNA.

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CloneID	OHu09108
Clone ID Related Accession (Same CDS sequence)	NM_138615.2 , XM_011533494.2 , XM_011533494.3 , XM_011533494.1 , NM_138615.3 , XM_011533495.2 , XM_011533495.1
Accession Version	XM_011533494.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX30 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011533494.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGAAAGT CACACTGCAC ATAAAATGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCTGAG CGGCAGGCTG CAGCTGCAGC CTGCCAGCTG 
TTCAAGGGTT GGGGTCTGCT AGGTCCCCGG AATGAGTTGT TTGACGCAGC CAAATACCGA 
GTGCTAGCTG ATCGCTTTGG CTCCCCTGCC GACAGCTGGT GGCGTCCGGA ACCCACCATG 
CCCCCTACTT CCTGGCGGCA GCTGAATCCA GAGAGTATTC GACCAGGGGG ACCTGGGGGC 
CTATCCCGCT CTTTAGGCCG GGAAGAAGAG GAGGACGAGG AGGAAGAGCT AGAAGAAGGG 
ACCATAGATG TTACCGACTT CTTGTCCATG ACCCAGCAGG ATTCCCACGC TCCACTCAGG 
GACTCAAGGG GGAGTTCCTT TGAGATGACA GATGACGACA GTGCCATTAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATTGCAAC GTCATCCTCC 
ACAGCTAAGA ACCTCATGCA GTTCCATACT GTGGGCACCA AGACCAAGCT GTCTACACTC 
ACCCTGCTCT GGCCCTGCCC CATGACCTTT GTTGCCAAAG GGCGCCGCAA AGCAGAGGCT 
GAGAATAAGG CGGCAGCCTT GGCCTGCAAG AAACTGAAGA GCCTGGGCCT GGTGGACAGG 
AACAACGAAC CGCTTACACA CGCCATGTAT AACCTGGCCT CTTTGCGTGA GCTGGGTGAG 
ACCCAGCGCC GACCATGCAC CATCCAGGTG CCCGAGCCCA TCCTCCGCAA GATAGAGACC 
TTCCTGAACC ATTACCCTGT GGAGAGTTCA TGGATCGCCC CAGAACTCCG GCTGCAGAGT 
GATGACATCT TGCCCTTGGG CAAGGACTCA GGGCCTCTGA GTGACCCTAT CACAGGCAAG 
CCCTATGTGC CCCTGTTGGA AGCAGAGGAG GTACGTCTCA GCCAGAGTCT GCTAGAACTG 
TGGCGGCGGC GAGGGCCGGT CTGGCAGGAG GCCCCCCAGC TACCTGTGGA CCCACATCGG 
GACACCATCC TCAACGCCAT TGAGCAGCAC CCGGTGGTGG TCATCTCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG TTGCTGGAGC GCTATGTGAC CGAGGGCCGA 
GGTGCCCGCT GCAATGTTAT CATCACCCAA CCTCGCCGCA TCTCTGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCACGAACT GGGCCCCTCC CTGCGCCGGA ATGTGGGCTT CCAGGTGCGG 
TTGGAAAGTA AGCCCCCATC CCGAGGCGGG GCCCTGCTCT TCTGCACTGT GGGTATCCTG 
CTGCGTAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTCAT CGTGGATGAG 
GTGCATGAGC GGGACGTGAA CACAGACTTT CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CCCTGCGGCT GGTGCTCATG AGTGCCACAG GGGACAATGA GCGCTTCTCC 
CGATACTTTG GTGGCTGCCC CGTCATCAAG GTGCCTGGCT TCATGTACCC AGTCAAGGAG 
CACTACCTAG AGGACATCCT GGCCAAGTTG GGCAAGCACC AGTACCTGCA CCGGCACCGG 
CACCATGAGT CTGAGGATGA ATGCGCACTC GATTTGGACC TTGTGACTGA TCTGGTTCTG 
CACATCGATG CTCGCGGGGA ACCAGGTGGG ATCCTGTGCT TCCTGCCTGG GTGGCAGGAG 
ATCAAAGGAG TGCAGCAGCG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCCATG ATGGATCAGA AGGCCATATT CCAGCAGCCT 
CCAGTTGGGG TGCGCAAGAT TGTCTTGGCC ACCAACATTG CTGAGACTTC CATCACAATC 
AATGACATCG TGCATGTGGT GGACAGTGGG CTGCACAAGG AAGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACAGTGTGG GTATCAAGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCCGGCTTT GCCTACCACT TGTTCCCTCG AAGCCGGCTG 
GAGAAAATGG TCCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCGA AAATCCACAT GCCTGAGAAG ACGGCGGTGG AGTTCCTGTC CAAGGCTGTG 
GACAGTCCAA ACATCAAGGC AGTGGACGAG GCTGTGATCT TGCTCCAGGA GATCGGGGTG 
CTGGACCAGC GGGAGTACCT GACTACCCTG GGGCAGCGCC TGGCTCACAT CTCCACCGAC 
CCCCGGTTGG CCAAGGCCAT TGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTACTG 
GTGGTCGTTT CCTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTACAGAA CCGGGCAGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAT GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCTGTCG CCGGCTGGGA GGAGGTGCTG CGTTGGCAGG ACCGCAGCTC CCGGGAGAAT 
TACCTGGAGG AAAACCTGCT GTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCCTTC CTGGTGGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCCGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG CGTGCTGATG 
GCCGGCCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTCACCCG GCAGGGGAAG 
TTCAAGCCCA ACAGCGTCAC ATATAGGACC AAATCAGGCA ACATCCTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC ACGGTTACGG AGCCGATGGC TGACGTATTT CATGGCAGTC 
AAGTCCAATG GCAGCGTCTT CGTCCGGGAC TCCTCTCAGG TGCACCCGCT AGCTGTGCTG 
CTGCTGACCG ACGGGGACGT GCACATCCGT GATGACGGGC GCCGGGCCAC CATCTCACTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGTA CCGTGCGGCT GCTGAAGGAG 
CTGCGGCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCTGCACTT 
CCCCCCAGCG TACAGGAGGA GCACGGGCAG CTGCTTGCGC TACTGGCAGA GCTGCTGCGA 
GGACCCTGTG GCAGCTTTGA TGTGCGCAAG ACAGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531796.1
CDS	315..3899
Translation	MFSLDSFRKDRAQHRQRQCKLPPPRLPPMCVNPTPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_011533494.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533494.3

ORF Insert Sequence:

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGAAAGT CACACTGCAC ATAAAATGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCTGAG CGGCAGGCTG CAGCTGCAGC CTGCCAGCTG 
TTCAAGGGTT GGGGTCTGCT AGGTCCCCGG AATGAGTTGT TTGACGCAGC CAAATACCGA 
GTGCTAGCTG ATCGCTTTGG CTCCCCTGCC GACAGCTGGT GGCGTCCGGA ACCCACCATG 
CCCCCTACTT CCTGGCGGCA GCTGAATCCA GAGAGTATTC GACCAGGGGG ACCTGGGGGC 
CTATCCCGCT CTTTAGGCCG GGAAGAAGAG GAGGACGAGG AGGAAGAGCT AGAAGAAGGG 
ACCATAGATG TTACCGACTT CTTGTCCATG ACCCAGCAGG ATTCCCACGC TCCACTCAGG 
GACTCAAGGG GGAGTTCCTT TGAGATGACA GATGACGACA GTGCCATTAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATTGCAAC GTCATCCTCC 
ACAGCTAAGA ACCTCATGCA GTTCCATACT GTGGGCACCA AGACCAAGCT GTCTACACTC 
ACCCTGCTCT GGCCCTGCCC CATGACCTTT GTTGCCAAAG GGCGCCGCAA AGCAGAGGCT 
GAGAATAAGG CGGCAGCCTT GGCCTGCAAG AAACTGAAGA GCCTGGGCCT GGTGGACAGG 
AACAACGAAC CGCTTACACA CGCCATGTAT AACCTGGCCT CTTTGCGTGA GCTGGGTGAG 
ACCCAGCGCC GACCATGCAC CATCCAGGTG CCCGAGCCCA TCCTCCGCAA GATAGAGACC 
TTCCTGAACC ATTACCCTGT GGAGAGTTCA TGGATCGCCC CAGAACTCCG GCTGCAGAGT 
GATGACATCT TGCCCTTGGG CAAGGACTCA GGGCCTCTGA GTGACCCTAT CACAGGCAAG 
CCCTATGTGC CCCTGTTGGA AGCAGAGGAG GTACGTCTCA GCCAGAGTCT GCTAGAACTG 
TGGCGGCGGC GAGGGCCGGT CTGGCAGGAG GCCCCCCAGC TACCTGTGGA CCCACATCGG 
GACACCATCC TCAACGCCAT TGAGCAGCAC CCGGTGGTGG TCATCTCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG TTGCTGGAGC GCTATGTGAC CGAGGGCCGA 
GGTGCCCGCT GCAATGTTAT CATCACCCAA CCTCGCCGCA TCTCTGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCACGAACT GGGCCCCTCC CTGCGCCGGA ATGTGGGCTT CCAGGTGCGG 
TTGGAAAGTA AGCCCCCATC CCGAGGCGGG GCCCTGCTCT TCTGCACTGT GGGTATCCTG 
CTGCGTAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTCAT CGTGGATGAG 
GTGCATGAGC GGGACGTGAA CACAGACTTT CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CCCTGCGGCT GGTGCTCATG AGTGCCACAG GGGACAATGA GCGCTTCTCC 
CGATACTTTG GTGGCTGCCC CGTCATCAAG GTGCCTGGCT TCATGTACCC AGTCAAGGAG 
CACTACCTAG AGGACATCCT GGCCAAGTTG GGCAAGCACC AGTACCTGCA CCGGCACCGG 
CACCATGAGT CTGAGGATGA ATGCGCACTC GATTTGGACC TTGTGACTGA TCTGGTTCTG 
CACATCGATG CTCGCGGGGA ACCAGGTGGG ATCCTGTGCT TCCTGCCTGG GTGGCAGGAG 
ATCAAAGGAG TGCAGCAGCG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCCATG ATGGATCAGA AGGCCATATT CCAGCAGCCT 
CCAGTTGGGG TGCGCAAGAT TGTCTTGGCC ACCAACATTG CTGAGACTTC CATCACAATC 
AATGACATCG TGCATGTGGT GGACAGTGGG CTGCACAAGG AAGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACAGTGTGG GTATCAAGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCCGGCTTT GCCTACCACT TGTTCCCTCG AAGCCGGCTG 
GAGAAAATGG TCCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCGA AAATCCACAT GCCTGAGAAG ACGGCGGTGG AGTTCCTGTC CAAGGCTGTG 
GACAGTCCAA ACATCAAGGC AGTGGACGAG GCTGTGATCT TGCTCCAGGA GATCGGGGTG 
CTGGACCAGC GGGAGTACCT GACTACCCTG GGGCAGCGCC TGGCTCACAT CTCCACCGAC 
CCCCGGTTGG CCAAGGCCAT TGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTACTG 
GTGGTCGTTT CCTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTACAGAA CCGGGCAGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAT GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCTGTCG CCGGCTGGGA GGAGGTGCTG CGTTGGCAGG ACCGCAGCTC CCGGGAGAAT 
TACCTGGAGG AAAACCTGCT GTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCCTTC CTGGTGGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCCGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG CGTGCTGATG 
GCCGGCCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTCACCCG GCAGGGGAAG 
TTCAAGCCCA ACAGCGTCAC ATATAGGACC AAATCAGGCA ACATCCTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC ACGGTTACGG AGCCGATGGC TGACGTATTT CATGGCAGTC 
AAGTCCAATG GCAGCGTCTT CGTCCGGGAC TCCTCTCAGG TGCACCCGCT AGCTGTGCTG 
CTGCTGACCG ACGGGGACGT GCACATCCGT GATGACGGGC GCCGGGCCAC CATCTCACTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGTA CCGTGCGGCT GCTGAAGGAG 
CTGCGGCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCTGCACTT 
CCCCCCAGCG TACAGGAGGA GCACGGGCAG CTGCTTGCGC TACTGGCAGA GCTGCTGCGA 
GGACCCTGTG GCAGCTTTGA TGTGCGCAAG ACAGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_011533494.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531796.1 Homo sapiens DEAH (Asp-Glu-Ala-His) box helicase 30 (DHX30), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu09108
Clone ID Related Accession (Same CDS sequence)	NM_138615.2 , XM_011533494.2 , XM_011533494.3 , XM_011533494.1 , NM_138615.3 , XM_011533495.2 , XM_011533495.1
Accession Version	XM_011533494.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	putative ATP-dependent RNA helicase DHX30 isoform X1
Comment

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGAAAGT CACACTGCAC ATAAAATGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCTGAG CGGCAGGCTG CAGCTGCAGC CTGCCAGCTG 
TTCAAGGGTT GGGGTCTGCT AGGTCCCCGG AATGAGTTGT TTGACGCAGC CAAATACCGA 
GTGCTAGCTG ATCGCTTTGG CTCCCCTGCC GACAGCTGGT GGCGTCCGGA ACCCACCATG 
CCCCCTACTT CCTGGCGGCA GCTGAATCCA GAGAGTATTC GACCAGGGGG ACCTGGGGGC 
CTATCCCGCT CTTTAGGCCG GGAAGAAGAG GAGGACGAGG AGGAAGAGCT AGAAGAAGGG 
ACCATAGATG TTACCGACTT CTTGTCCATG ACCCAGCAGG ATTCCCACGC TCCACTCAGG 
GACTCAAGGG GGAGTTCCTT TGAGATGACA GATGACGACA GTGCCATTAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATTGCAAC GTCATCCTCC 
ACAGCTAAGA ACCTCATGCA GTTCCATACT GTGGGCACCA AGACCAAGCT GTCTACACTC 
ACCCTGCTCT GGCCCTGCCC CATGACCTTT GTTGCCAAAG GGCGCCGCAA AGCAGAGGCT 
GAGAATAAGG CGGCAGCCTT GGCCTGCAAG AAACTGAAGA GCCTGGGCCT GGTGGACAGG 
AACAACGAAC CGCTTACACA CGCCATGTAT AACCTGGCCT CTTTGCGTGA GCTGGGTGAG 
ACCCAGCGCC GACCATGCAC CATCCAGGTG CCCGAGCCCA TCCTCCGCAA GATAGAGACC 
TTCCTGAACC ATTACCCTGT GGAGAGTTCA TGGATCGCCC CAGAACTCCG GCTGCAGAGT 
GATGACATCT TGCCCTTGGG CAAGGACTCA GGGCCTCTGA GTGACCCTAT CACAGGCAAG 
CCCTATGTGC CCCTGTTGGA AGCAGAGGAG GTACGTCTCA GCCAGAGTCT GCTAGAACTG 
TGGCGGCGGC GAGGGCCGGT CTGGCAGGAG GCCCCCCAGC TACCTGTGGA CCCACATCGG 
GACACCATCC TCAACGCCAT TGAGCAGCAC CCGGTGGTGG TCATCTCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG TTGCTGGAGC GCTATGTGAC CGAGGGCCGA 
GGTGCCCGCT GCAATGTTAT CATCACCCAA CCTCGCCGCA TCTCTGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCACGAACT GGGCCCCTCC CTGCGCCGGA ATGTGGGCTT CCAGGTGCGG 
TTGGAAAGTA AGCCCCCATC CCGAGGCGGG GCCCTGCTCT TCTGCACTGT GGGTATCCTG 
CTGCGTAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTCAT CGTGGATGAG 
GTGCATGAGC GGGACGTGAA CACAGACTTT CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CCCTGCGGCT GGTGCTCATG AGTGCCACAG GGGACAATGA GCGCTTCTCC 
CGATACTTTG GTGGCTGCCC CGTCATCAAG GTGCCTGGCT TCATGTACCC AGTCAAGGAG 
CACTACCTAG AGGACATCCT GGCCAAGTTG GGCAAGCACC AGTACCTGCA CCGGCACCGG 
CACCATGAGT CTGAGGATGA ATGCGCACTC GATTTGGACC TTGTGACTGA TCTGGTTCTG 
CACATCGATG CTCGCGGGGA ACCAGGTGGG ATCCTGTGCT TCCTGCCTGG GTGGCAGGAG 
ATCAAAGGAG TGCAGCAGCG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCCATG ATGGATCAGA AGGCCATATT CCAGCAGCCT 
CCAGTTGGGG TGCGCAAGAT TGTCTTGGCC ACCAACATTG CTGAGACTTC CATCACAATC 
AATGACATCG TGCATGTGGT GGACAGTGGG CTGCACAAGG AAGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACAGTGTGG GTATCAAGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCCGGCTTT GCCTACCACT TGTTCCCTCG AAGCCGGCTG 
GAGAAAATGG TCCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCGA AAATCCACAT GCCTGAGAAG ACGGCGGTGG AGTTCCTGTC CAAGGCTGTG 
GACAGTCCAA ACATCAAGGC AGTGGACGAG GCTGTGATCT TGCTCCAGGA GATCGGGGTG 
CTGGACCAGC GGGAGTACCT GACTACCCTG GGGCAGCGCC TGGCTCACAT CTCCACCGAC 
CCCCGGTTGG CCAAGGCCAT TGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTACTG 
GTGGTCGTTT CCTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTACAGAA CCGGGCAGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAT GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCTGTCG CCGGCTGGGA GGAGGTGCTG CGTTGGCAGG ACCGCAGCTC CCGGGAGAAT 
TACCTGGAGG AAAACCTGCT GTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCCTTC CTGGTGGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCCGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG CGTGCTGATG 
GCCGGCCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTCACCCG GCAGGGGAAG 
TTCAAGCCCA ACAGCGTCAC ATATAGGACC AAATCAGGCA ACATCCTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC ACGGTTACGG AGCCGATGGC TGACGTATTT CATGGCAGTC 
AAGTCCAATG GCAGCGTCTT CGTCCGGGAC TCCTCTCAGG TGCACCCGCT AGCTGTGCTG 
CTGCTGACCG ACGGGGACGT GCACATCCGT GATGACGGGC GCCGGGCCAC CATCTCACTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGTA CCGTGCGGCT GCTGAAGGAG 
CTGCGGCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCTGCACTT 
CCCCCCAGCG TACAGGAGGA GCACGGGCAG CTGCTTGCGC TACTGGCAGA GCTGCTGCGA 
GGACCCTGTG GCAGCTTTGA TGTGCGCAAG ACAGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011531796.1

Translation

MFSLDSFRKDRAQHRQRQCKLPPPRLPPMCVNPTPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_011533494.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens DEAH (Asp-Glu-Ala-His) box helicase 30 (DHX30), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533494.1

ORF Insert Sequence:

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGAAAGT CACACTGCAC ATAAAATGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCTGAG CGGCAGGCTG CAGCTGCAGC CTGCCAGCTG 
TTCAAGGGTT GGGGTCTGCT AGGTCCCCGG AATGAGTTGT TTGACGCAGC CAAATACCGA 
GTGCTAGCTG ATCGCTTTGG CTCCCCTGCC GACAGCTGGT GGCGTCCGGA ACCCACCATG 
CCCCCTACTT CCTGGCGGCA GCTGAATCCA GAGAGTATTC GACCAGGGGG ACCTGGGGGC 
CTATCCCGCT CTTTAGGCCG GGAAGAAGAG GAGGACGAGG AGGAAGAGCT AGAAGAAGGG 
ACCATAGATG TTACCGACTT CTTGTCCATG ACCCAGCAGG ATTCCCACGC TCCACTCAGG 
GACTCAAGGG GGAGTTCCTT TGAGATGACA GATGACGACA GTGCCATTAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATTGCAAC GTCATCCTCC 
ACAGCTAAGA ACCTCATGCA GTTCCATACT GTGGGCACCA AGACCAAGCT GTCTACACTC 
ACCCTGCTCT GGCCCTGCCC CATGACCTTT GTTGCCAAAG GGCGCCGCAA AGCAGAGGCT 
GAGAATAAGG CGGCAGCCTT GGCCTGCAAG AAACTGAAGA GCCTGGGCCT GGTGGACAGG 
AACAACGAAC CGCTTACACA CGCCATGTAT AACCTGGCCT CTTTGCGTGA GCTGGGTGAG 
ACCCAGCGCC GACCATGCAC CATCCAGGTG CCCGAGCCCA TCCTCCGCAA GATAGAGACC 
TTCCTGAACC ATTACCCTGT GGAGAGTTCA TGGATCGCCC CAGAACTCCG GCTGCAGAGT 
GATGACATCT TGCCCTTGGG CAAGGACTCA GGGCCTCTGA GTGACCCTAT CACAGGCAAG 
CCCTATGTGC CCCTGTTGGA AGCAGAGGAG GTACGTCTCA GCCAGAGTCT GCTAGAACTG 
TGGCGGCGGC GAGGGCCGGT CTGGCAGGAG GCCCCCCAGC TACCTGTGGA CCCACATCGG 
GACACCATCC TCAACGCCAT TGAGCAGCAC CCGGTGGTGG TCATCTCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG TTGCTGGAGC GCTATGTGAC CGAGGGCCGA 
GGTGCCCGCT GCAATGTTAT CATCACCCAA CCTCGCCGCA TCTCTGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCACGAACT GGGCCCCTCC CTGCGCCGGA ATGTGGGCTT CCAGGTGCGG 
TTGGAAAGTA AGCCCCCATC CCGAGGCGGG GCCCTGCTCT TCTGCACTGT GGGTATCCTG 
CTGCGTAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTCAT CGTGGATGAG 
GTGCATGAGC GGGACGTGAA CACAGACTTT CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CCCTGCGGCT GGTGCTCATG AGTGCCACAG GGGACAATGA GCGCTTCTCC 
CGATACTTTG GTGGCTGCCC CGTCATCAAG GTGCCTGGCT TCATGTACCC AGTCAAGGAG 
CACTACCTAG AGGACATCCT GGCCAAGTTG GGCAAGCACC AGTACCTGCA CCGGCACCGG 
CACCATGAGT CTGAGGATGA ATGCGCACTC GATTTGGACC TTGTGACTGA TCTGGTTCTG 
CACATCGATG CTCGCGGGGA ACCAGGTGGG ATCCTGTGCT TCCTGCCTGG GTGGCAGGAG 
ATCAAAGGAG TGCAGCAGCG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCCATG ATGGATCAGA AGGCCATATT CCAGCAGCCT 
CCAGTTGGGG TGCGCAAGAT TGTCTTGGCC ACCAACATTG CTGAGACTTC CATCACAATC 
AATGACATCG TGCATGTGGT GGACAGTGGG CTGCACAAGG AAGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACAGTGTGG GTATCAAGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCCGGCTTT GCCTACCACT TGTTCCCTCG AAGCCGGCTG 
GAGAAAATGG TCCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCGA AAATCCACAT GCCTGAGAAG ACGGCGGTGG AGTTCCTGTC CAAGGCTGTG 
GACAGTCCAA ACATCAAGGC AGTGGACGAG GCTGTGATCT TGCTCCAGGA GATCGGGGTG 
CTGGACCAGC GGGAGTACCT GACTACCCTG GGGCAGCGCC TGGCTCACAT CTCCACCGAC 
CCCCGGTTGG CCAAGGCCAT TGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTACTG 
GTGGTCGTTT CCTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTACAGAA CCGGGCAGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAT GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCTGTCG CCGGCTGGGA GGAGGTGCTG CGTTGGCAGG ACCGCAGCTC CCGGGAGAAT 
TACCTGGAGG AAAACCTGCT GTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCCTTC CTGGTGGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCCGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG CGTGCTGATG 
GCCGGCCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTCACCCG GCAGGGGAAG 
TTCAAGCCCA ACAGCGTCAC ATATAGGACC AAATCAGGCA ACATCCTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC ACGGTTACGG AGCCGATGGC TGACGTATTT CATGGCAGTC 
AAGTCCAATG GCAGCGTCTT CGTCCGGGAC TCCTCTCAGG TGCACCCGCT AGCTGTGCTG 
CTGCTGACCG ACGGGGACGT GCACATCCGT GATGACGGGC GCCGGGCCAC CATCTCACTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGTA CCGTGCGGCT GCTGAAGGAG 
CTGCGGCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCTGCACTT 
CCCCCCAGCG TACAGGAGGA GCACGGGCAG CTGCTTGCGC TACTGGCAGA GCTGCTGCGA 
GGACCCTGTG GCAGCTTTGA TGTGCGCAAG ACAGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_011533495.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531797.1 Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X5, mRNA.

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CloneID	OHu09108
Clone ID Related Accession (Same CDS sequence)	NM_138615.2 , XM_011533494.2 , XM_011533494.3 , XM_011533494.1 , NM_138615.3 , XM_011533495.2 , XM_011533495.1
Accession Version	XM_011533495.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX30 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011533495.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGAAAGT CACACTGCAC ATAAAATGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCTGAG CGGCAGGCTG CAGCTGCAGC CTGCCAGCTG 
TTCAAGGGTT GGGGTCTGCT AGGTCCCCGG AATGAGTTGT TTGACGCAGC CAAATACCGA 
GTGCTAGCTG ATCGCTTTGG CTCCCCTGCC GACAGCTGGT GGCGTCCGGA ACCCACCATG 
CCCCCTACTT CCTGGCGGCA GCTGAATCCA GAGAGTATTC GACCAGGGGG ACCTGGGGGC 
CTATCCCGCT CTTTAGGCCG GGAAGAAGAG GAGGACGAGG AGGAAGAGCT AGAAGAAGGG 
ACCATAGATG TTACCGACTT CTTGTCCATG ACCCAGCAGG ATTCCCACGC TCCACTCAGG 
GACTCAAGGG GGAGTTCCTT TGAGATGACA GATGACGACA GTGCCATTAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATTGCAAC GTCATCCTCC 
ACAGCTAAGA ACCTCATGCA GTTCCATACT GTGGGCACCA AGACCAAGCT GTCTACACTC 
ACCCTGCTCT GGCCCTGCCC CATGACCTTT GTTGCCAAAG GGCGCCGCAA AGCAGAGGCT 
GAGAATAAGG CGGCAGCCTT GGCCTGCAAG AAACTGAAGA GCCTGGGCCT GGTGGACAGG 
AACAACGAAC CGCTTACACA CGCCATGTAT AACCTGGCCT CTTTGCGTGA GCTGGGTGAG 
ACCCAGCGCC GACCATGCAC CATCCAGGTG CCCGAGCCCA TCCTCCGCAA GATAGAGACC 
TTCCTGAACC ATTACCCTGT GGAGAGTTCA TGGATCGCCC CAGAACTCCG GCTGCAGAGT 
GATGACATCT TGCCCTTGGG CAAGGACTCA GGGCCTCTGA GTGACCCTAT CACAGGCAAG 
CCCTATGTGC CCCTGTTGGA AGCAGAGGAG GTACGTCTCA GCCAGAGTCT GCTAGAACTG 
TGGCGGCGGC GAGGGCCGGT CTGGCAGGAG GCCCCCCAGC TACCTGTGGA CCCACATCGG 
GACACCATCC TCAACGCCAT TGAGCAGCAC CCGGTGGTGG TCATCTCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG TTGCTGGAGC GCTATGTGAC CGAGGGCCGA 
GGTGCCCGCT GCAATGTTAT CATCACCCAA CCTCGCCGCA TCTCTGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCACGAACT GGGCCCCTCC CTGCGCCGGA ATGTGGGCTT CCAGGTGCGG 
TTGGAAAGTA AGCCCCCATC CCGAGGCGGG GCCCTGCTCT TCTGCACTGT GGGTATCCTG 
CTGCGTAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTCAT CGTGGATGAG 
GTGCATGAGC GGGACGTGAA CACAGACTTT CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CCCTGCGGCT GGTGCTCATG AGTGCCACAG GGGACAATGA GCGCTTCTCC 
CGATACTTTG GTGGCTGCCC CGTCATCAAG GTGCCTGGCT TCATGTACCC AGTCAAGGAG 
CACTACCTAG AGGACATCCT GGCCAAGTTG GGCAAGCACC AGTACCTGCA CCGGCACCGG 
CACCATGAGT CTGAGGATGA ATGCGCACTC GATTTGGACC TTGTGACTGA TCTGGTTCTG 
CACATCGATG CTCGCGGGGA ACCAGGTGGG ATCCTGTGCT TCCTGCCTGG GTGGCAGGAG 
ATCAAAGGAG TGCAGCAGCG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCCATG ATGGATCAGA AGGCCATATT CCAGCAGCCT 
CCAGTTGGGG TGCGCAAGAT TGTCTTGGCC ACCAACATTG CTGAGACTTC CATCACAATC 
AATGACATCG TGCATGTGGT GGACAGTGGG CTGCACAAGG AAGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACAGTGTGG GTATCAAGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCCGGCTTT GCCTACCACT TGTTCCCTCG AAGCCGGCTG 
GAGAAAATGG TCCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCGA AAATCCACAT GCCTGAGAAG ACGGCGGTGG AGTTCCTGTC CAAGGCTGTG 
GACAGTCCAA ACATCAAGGC AGTGGACGAG GCTGTGATCT TGCTCCAGGA GATCGGGGTG 
CTGGACCAGC GGGAGTACCT GACTACCCTG GGGCAGCGCC TGGCTCACAT CTCCACCGAC 
CCCCGGTTGG CCAAGGCCAT TGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTACTG 
GTGGTCGTTT CCTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTACAGAA CCGGGCAGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAT GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCTGTCG CCGGCTGGGA GGAGGTGCTG CGTTGGCAGG ACCGCAGCTC CCGGGAGAAT 
TACCTGGAGG AAAACCTGCT GTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCCTTC CTGGTGGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCCGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG CGTGCTGATG 
GCCGGCCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTCACCCG GCAGGGGAAG 
TTCAAGCCCA ACAGCGTCAC ATATAGGACC AAATCAGGCA ACATCCTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC ACGGTTACGG AGCCGATGGC TGACGTATTT CATGGCAGTC 
AAGTCCAATG GCAGCGTCTT CGTCCGGGAC TCCTCTCAGG TGCACCCGCT AGCTGTGCTG 
CTGCTGACCG ACGGGGACGT GCACATCCGT GATGACGGGC GCCGGGCCAC CATCTCACTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGTA CCGTGCGGCT GCTGAAGGAG 
CTGCGGCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCTGCACTT 
CCCCCCAGCG TACAGGAGGA GCACGGGCAG CTGCTTGCGC TACTGGCAGA GCTGCTGCGA 
GGACCCTGTG GCAGCTTTGA TGTGCGCAAG ACAGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531797.1
CDS	303..3887
Translation	MFSLDSFRKDRAQHRQRQCKLPPPRLPPMCVNPTPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_011533495.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533495.2

ORF Insert Sequence:

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGAAAGT CACACTGCAC ATAAAATGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCTGAG CGGCAGGCTG CAGCTGCAGC CTGCCAGCTG 
TTCAAGGGTT GGGGTCTGCT AGGTCCCCGG AATGAGTTGT TTGACGCAGC CAAATACCGA 
GTGCTAGCTG ATCGCTTTGG CTCCCCTGCC GACAGCTGGT GGCGTCCGGA ACCCACCATG 
CCCCCTACTT CCTGGCGGCA GCTGAATCCA GAGAGTATTC GACCAGGGGG ACCTGGGGGC 
CTATCCCGCT CTTTAGGCCG GGAAGAAGAG GAGGACGAGG AGGAAGAGCT AGAAGAAGGG 
ACCATAGATG TTACCGACTT CTTGTCCATG ACCCAGCAGG ATTCCCACGC TCCACTCAGG 
GACTCAAGGG GGAGTTCCTT TGAGATGACA GATGACGACA GTGCCATTAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATTGCAAC GTCATCCTCC 
ACAGCTAAGA ACCTCATGCA GTTCCATACT GTGGGCACCA AGACCAAGCT GTCTACACTC 
ACCCTGCTCT GGCCCTGCCC CATGACCTTT GTTGCCAAAG GGCGCCGCAA AGCAGAGGCT 
GAGAATAAGG CGGCAGCCTT GGCCTGCAAG AAACTGAAGA GCCTGGGCCT GGTGGACAGG 
AACAACGAAC CGCTTACACA CGCCATGTAT AACCTGGCCT CTTTGCGTGA GCTGGGTGAG 
ACCCAGCGCC GACCATGCAC CATCCAGGTG CCCGAGCCCA TCCTCCGCAA GATAGAGACC 
TTCCTGAACC ATTACCCTGT GGAGAGTTCA TGGATCGCCC CAGAACTCCG GCTGCAGAGT 
GATGACATCT TGCCCTTGGG CAAGGACTCA GGGCCTCTGA GTGACCCTAT CACAGGCAAG 
CCCTATGTGC CCCTGTTGGA AGCAGAGGAG GTACGTCTCA GCCAGAGTCT GCTAGAACTG 
TGGCGGCGGC GAGGGCCGGT CTGGCAGGAG GCCCCCCAGC TACCTGTGGA CCCACATCGG 
GACACCATCC TCAACGCCAT TGAGCAGCAC CCGGTGGTGG TCATCTCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG TTGCTGGAGC GCTATGTGAC CGAGGGCCGA 
GGTGCCCGCT GCAATGTTAT CATCACCCAA CCTCGCCGCA TCTCTGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCACGAACT GGGCCCCTCC CTGCGCCGGA ATGTGGGCTT CCAGGTGCGG 
TTGGAAAGTA AGCCCCCATC CCGAGGCGGG GCCCTGCTCT TCTGCACTGT GGGTATCCTG 
CTGCGTAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTCAT CGTGGATGAG 
GTGCATGAGC GGGACGTGAA CACAGACTTT CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CCCTGCGGCT GGTGCTCATG AGTGCCACAG GGGACAATGA GCGCTTCTCC 
CGATACTTTG GTGGCTGCCC CGTCATCAAG GTGCCTGGCT TCATGTACCC AGTCAAGGAG 
CACTACCTAG AGGACATCCT GGCCAAGTTG GGCAAGCACC AGTACCTGCA CCGGCACCGG 
CACCATGAGT CTGAGGATGA ATGCGCACTC GATTTGGACC TTGTGACTGA TCTGGTTCTG 
CACATCGATG CTCGCGGGGA ACCAGGTGGG ATCCTGTGCT TCCTGCCTGG GTGGCAGGAG 
ATCAAAGGAG TGCAGCAGCG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCCATG ATGGATCAGA AGGCCATATT CCAGCAGCCT 
CCAGTTGGGG TGCGCAAGAT TGTCTTGGCC ACCAACATTG CTGAGACTTC CATCACAATC 
AATGACATCG TGCATGTGGT GGACAGTGGG CTGCACAAGG AAGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACAGTGTGG GTATCAAGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCCGGCTTT GCCTACCACT TGTTCCCTCG AAGCCGGCTG 
GAGAAAATGG TCCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCGA AAATCCACAT GCCTGAGAAG ACGGCGGTGG AGTTCCTGTC CAAGGCTGTG 
GACAGTCCAA ACATCAAGGC AGTGGACGAG GCTGTGATCT TGCTCCAGGA GATCGGGGTG 
CTGGACCAGC GGGAGTACCT GACTACCCTG GGGCAGCGCC TGGCTCACAT CTCCACCGAC 
CCCCGGTTGG CCAAGGCCAT TGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTACTG 
GTGGTCGTTT CCTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTACAGAA CCGGGCAGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAT GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCTGTCG CCGGCTGGGA GGAGGTGCTG CGTTGGCAGG ACCGCAGCTC CCGGGAGAAT 
TACCTGGAGG AAAACCTGCT GTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCCTTC CTGGTGGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCCGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG CGTGCTGATG 
GCCGGCCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTCACCCG GCAGGGGAAG 
TTCAAGCCCA ACAGCGTCAC ATATAGGACC AAATCAGGCA ACATCCTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC ACGGTTACGG AGCCGATGGC TGACGTATTT CATGGCAGTC 
AAGTCCAATG GCAGCGTCTT CGTCCGGGAC TCCTCTCAGG TGCACCCGCT AGCTGTGCTG 
CTGCTGACCG ACGGGGACGT GCACATCCGT GATGACGGGC GCCGGGCCAC CATCTCACTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGTA CCGTGCGGCT GCTGAAGGAG 
CTGCGGCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCTGCACTT 
CCCCCCAGCG TACAGGAGGA GCACGGGCAG CTGCTTGCGC TACTGGCAGA GCTGCTGCGA 
GGACCCTGTG GCAGCTTTGA TGTGCGCAAG ACAGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_011533495.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531797.1 Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X7, mRNA.

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CloneID	OHu09108
Clone ID Related Accession (Same CDS sequence)	NM_138615.2 , XM_011533494.2 , XM_011533494.3 , XM_011533494.1 , NM_138615.3 , XM_011533495.2 , XM_011533495.1
Accession Version	XM_011533495.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative ATP-dependent RNA helicase DHX30 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGAAAGT CACACTGCAC ATAAAATGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCTGAG CGGCAGGCTG CAGCTGCAGC CTGCCAGCTG 
TTCAAGGGTT GGGGTCTGCT AGGTCCCCGG AATGAGTTGT TTGACGCAGC CAAATACCGA 
GTGCTAGCTG ATCGCTTTGG CTCCCCTGCC GACAGCTGGT GGCGTCCGGA ACCCACCATG 
CCCCCTACTT CCTGGCGGCA GCTGAATCCA GAGAGTATTC GACCAGGGGG ACCTGGGGGC 
CTATCCCGCT CTTTAGGCCG GGAAGAAGAG GAGGACGAGG AGGAAGAGCT AGAAGAAGGG 
ACCATAGATG TTACCGACTT CTTGTCCATG ACCCAGCAGG ATTCCCACGC TCCACTCAGG 
GACTCAAGGG GGAGTTCCTT TGAGATGACA GATGACGACA GTGCCATTAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATTGCAAC GTCATCCTCC 
ACAGCTAAGA ACCTCATGCA GTTCCATACT GTGGGCACCA AGACCAAGCT GTCTACACTC 
ACCCTGCTCT GGCCCTGCCC CATGACCTTT GTTGCCAAAG GGCGCCGCAA AGCAGAGGCT 
GAGAATAAGG CGGCAGCCTT GGCCTGCAAG AAACTGAAGA GCCTGGGCCT GGTGGACAGG 
AACAACGAAC CGCTTACACA CGCCATGTAT AACCTGGCCT CTTTGCGTGA GCTGGGTGAG 
ACCCAGCGCC GACCATGCAC CATCCAGGTG CCCGAGCCCA TCCTCCGCAA GATAGAGACC 
TTCCTGAACC ATTACCCTGT GGAGAGTTCA TGGATCGCCC CAGAACTCCG GCTGCAGAGT 
GATGACATCT TGCCCTTGGG CAAGGACTCA GGGCCTCTGA GTGACCCTAT CACAGGCAAG 
CCCTATGTGC CCCTGTTGGA AGCAGAGGAG GTACGTCTCA GCCAGAGTCT GCTAGAACTG 
TGGCGGCGGC GAGGGCCGGT CTGGCAGGAG GCCCCCCAGC TACCTGTGGA CCCACATCGG 
GACACCATCC TCAACGCCAT TGAGCAGCAC CCGGTGGTGG TCATCTCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG TTGCTGGAGC GCTATGTGAC CGAGGGCCGA 
GGTGCCCGCT GCAATGTTAT CATCACCCAA CCTCGCCGCA TCTCTGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCACGAACT GGGCCCCTCC CTGCGCCGGA ATGTGGGCTT CCAGGTGCGG 
TTGGAAAGTA AGCCCCCATC CCGAGGCGGG GCCCTGCTCT TCTGCACTGT GGGTATCCTG 
CTGCGTAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTCAT CGTGGATGAG 
GTGCATGAGC GGGACGTGAA CACAGACTTT CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CCCTGCGGCT GGTGCTCATG AGTGCCACAG GGGACAATGA GCGCTTCTCC 
CGATACTTTG GTGGCTGCCC CGTCATCAAG GTGCCTGGCT TCATGTACCC AGTCAAGGAG 
CACTACCTAG AGGACATCCT GGCCAAGTTG GGCAAGCACC AGTACCTGCA CCGGCACCGG 
CACCATGAGT CTGAGGATGA ATGCGCACTC GATTTGGACC TTGTGACTGA TCTGGTTCTG 
CACATCGATG CTCGCGGGGA ACCAGGTGGG ATCCTGTGCT TCCTGCCTGG GTGGCAGGAG 
ATCAAAGGAG TGCAGCAGCG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCCATG ATGGATCAGA AGGCCATATT CCAGCAGCCT 
CCAGTTGGGG TGCGCAAGAT TGTCTTGGCC ACCAACATTG CTGAGACTTC CATCACAATC 
AATGACATCG TGCATGTGGT GGACAGTGGG CTGCACAAGG AAGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACAGTGTGG GTATCAAGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCCGGCTTT GCCTACCACT TGTTCCCTCG AAGCCGGCTG 
GAGAAAATGG TCCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCGA AAATCCACAT GCCTGAGAAG ACGGCGGTGG AGTTCCTGTC CAAGGCTGTG 
GACAGTCCAA ACATCAAGGC AGTGGACGAG GCTGTGATCT TGCTCCAGGA GATCGGGGTG 
CTGGACCAGC GGGAGTACCT GACTACCCTG GGGCAGCGCC TGGCTCACAT CTCCACCGAC 
CCCCGGTTGG CCAAGGCCAT TGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTACTG 
GTGGTCGTTT CCTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTACAGAA CCGGGCAGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAT GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCTGTCG CCGGCTGGGA GGAGGTGCTG CGTTGGCAGG ACCGCAGCTC CCGGGAGAAT 
TACCTGGAGG AAAACCTGCT GTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCCTTC CTGGTGGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCCGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG CGTGCTGATG 
GCCGGCCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTCACCCG GCAGGGGAAG 
TTCAAGCCCA ACAGCGTCAC ATATAGGACC AAATCAGGCA ACATCCTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC ACGGTTACGG AGCCGATGGC TGACGTATTT CATGGCAGTC 
AAGTCCAATG GCAGCGTCTT CGTCCGGGAC TCCTCTCAGG TGCACCCGCT AGCTGTGCTG 
CTGCTGACCG ACGGGGACGT GCACATCCGT GATGACGGGC GCCGGGCCAC CATCTCACTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGTA CCGTGCGGCT GCTGAAGGAG 
CTGCGGCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCTGCACTT 
CCCCCCAGCG TACAGGAGGA GCACGGGCAG CTGCTTGCGC TACTGGCAGA GCTGCTGCGA 
GGACCCTGTG GCAGCTTTGA TGTGCGCAAG ACAGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531797.1
CDS	236..3820
Translation	MFSLDSFRKDRAQHRQRQCKLPPPRLPPMCVNPTPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_011533495.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533495.1

ORF Insert Sequence:

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGAAAGT CACACTGCAC ATAAAATGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCTGAG CGGCAGGCTG CAGCTGCAGC CTGCCAGCTG 
TTCAAGGGTT GGGGTCTGCT AGGTCCCCGG AATGAGTTGT TTGACGCAGC CAAATACCGA 
GTGCTAGCTG ATCGCTTTGG CTCCCCTGCC GACAGCTGGT GGCGTCCGGA ACCCACCATG 
CCCCCTACTT CCTGGCGGCA GCTGAATCCA GAGAGTATTC GACCAGGGGG ACCTGGGGGC 
CTATCCCGCT CTTTAGGCCG GGAAGAAGAG GAGGACGAGG AGGAAGAGCT AGAAGAAGGG 
ACCATAGATG TTACCGACTT CTTGTCCATG ACCCAGCAGG ATTCCCACGC TCCACTCAGG 
GACTCAAGGG GGAGTTCCTT TGAGATGACA GATGACGACA GTGCCATTAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATTGCAAC GTCATCCTCC 
ACAGCTAAGA ACCTCATGCA GTTCCATACT GTGGGCACCA AGACCAAGCT GTCTACACTC 
ACCCTGCTCT GGCCCTGCCC CATGACCTTT GTTGCCAAAG GGCGCCGCAA AGCAGAGGCT 
GAGAATAAGG CGGCAGCCTT GGCCTGCAAG AAACTGAAGA GCCTGGGCCT GGTGGACAGG 
AACAACGAAC CGCTTACACA CGCCATGTAT AACCTGGCCT CTTTGCGTGA GCTGGGTGAG 
ACCCAGCGCC GACCATGCAC CATCCAGGTG CCCGAGCCCA TCCTCCGCAA GATAGAGACC 
TTCCTGAACC ATTACCCTGT GGAGAGTTCA TGGATCGCCC CAGAACTCCG GCTGCAGAGT 
GATGACATCT TGCCCTTGGG CAAGGACTCA GGGCCTCTGA GTGACCCTAT CACAGGCAAG 
CCCTATGTGC CCCTGTTGGA AGCAGAGGAG GTACGTCTCA GCCAGAGTCT GCTAGAACTG 
TGGCGGCGGC GAGGGCCGGT CTGGCAGGAG GCCCCCCAGC TACCTGTGGA CCCACATCGG 
GACACCATCC TCAACGCCAT TGAGCAGCAC CCGGTGGTGG TCATCTCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG TTGCTGGAGC GCTATGTGAC CGAGGGCCGA 
GGTGCCCGCT GCAATGTTAT CATCACCCAA CCTCGCCGCA TCTCTGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCACGAACT GGGCCCCTCC CTGCGCCGGA ATGTGGGCTT CCAGGTGCGG 
TTGGAAAGTA AGCCCCCATC CCGAGGCGGG GCCCTGCTCT TCTGCACTGT GGGTATCCTG 
CTGCGTAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTCAT CGTGGATGAG 
GTGCATGAGC GGGACGTGAA CACAGACTTT CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CCCTGCGGCT GGTGCTCATG AGTGCCACAG GGGACAATGA GCGCTTCTCC 
CGATACTTTG GTGGCTGCCC CGTCATCAAG GTGCCTGGCT TCATGTACCC AGTCAAGGAG 
CACTACCTAG AGGACATCCT GGCCAAGTTG GGCAAGCACC AGTACCTGCA CCGGCACCGG 
CACCATGAGT CTGAGGATGA ATGCGCACTC GATTTGGACC TTGTGACTGA TCTGGTTCTG 
CACATCGATG CTCGCGGGGA ACCAGGTGGG ATCCTGTGCT TCCTGCCTGG GTGGCAGGAG 
ATCAAAGGAG TGCAGCAGCG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCCATG ATGGATCAGA AGGCCATATT CCAGCAGCCT 
CCAGTTGGGG TGCGCAAGAT TGTCTTGGCC ACCAACATTG CTGAGACTTC CATCACAATC 
AATGACATCG TGCATGTGGT GGACAGTGGG CTGCACAAGG AAGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACAGTGTGG GTATCAAGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCCGGCTTT GCCTACCACT TGTTCCCTCG AAGCCGGCTG 
GAGAAAATGG TCCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCGA AAATCCACAT GCCTGAGAAG ACGGCGGTGG AGTTCCTGTC CAAGGCTGTG 
GACAGTCCAA ACATCAAGGC AGTGGACGAG GCTGTGATCT TGCTCCAGGA GATCGGGGTG 
CTGGACCAGC GGGAGTACCT GACTACCCTG GGGCAGCGCC TGGCTCACAT CTCCACCGAC 
CCCCGGTTGG CCAAGGCCAT TGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTACTG 
GTGGTCGTTT CCTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTACAGAA CCGGGCAGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAT GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCTGTCG CCGGCTGGGA GGAGGTGCTG CGTTGGCAGG ACCGCAGCTC CCGGGAGAAT 
TACCTGGAGG AAAACCTGCT GTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCCTTC CTGGTGGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCCGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG CGTGCTGATG 
GCCGGCCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTCACCCG GCAGGGGAAG 
TTCAAGCCCA ACAGCGTCAC ATATAGGACC AAATCAGGCA ACATCCTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC ACGGTTACGG AGCCGATGGC TGACGTATTT CATGGCAGTC 
AAGTCCAATG GCAGCGTCTT CGTCCGGGAC TCCTCTCAGG TGCACCCGCT AGCTGTGCTG 
CTGCTGACCG ACGGGGACGT GCACATCCGT GATGACGGGC GCCGGGCCAC CATCTCACTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGTA CCGTGCGGCT GCTGAAGGAG 
CTGCGGCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCTGCACTT 
CCCCCCAGCG TACAGGAGGA GCACGGGCAG CTGCTTGCGC TACTGGCAGA GCTGCTGCGA 
GGACCCTGTG GCAGCTTTGA TGTGCGCAAG ACAGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_011533497.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531799.1 Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X6, mRNA.

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CloneID	OHu65127
Clone ID Related Accession (Same CDS sequence)	XM_011533497.2 , XM_011533498.1 , XM_011533496.1 , NM_001330990.1
Accession Version	XM_011533497.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX30 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533497.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTCA ACCCTACCCC AGGAGGGACC ATCTCTCGAG CTTCTAGGGA CCTATTAAAA 
GAGTTCCCAC AGCCCAAAAA TCTTCTCAAC AGTGTGATTG GAAGAGCCCT CGGCATCTCA 
CATGCAAAAG ACAAACTAGT CTACGTGCAC ACAAATGGAC CGAAGAAAAA GAAAGTCACA 
CTGCACATAA AATGGCCCAA GAGCGTGGAG GTAGAAGGCT ATGGCAGCAA GAAGATCGAT 
GCTGAGCGGC AGGCTGCAGC TGCAGCCTGC CAGCTGTTCA AGGGTTGGGG TCTGCTAGGT 
CCCCGGAATG AGTTGTTTGA CGCAGCCAAA TACCGAGTGC TAGCTGATCG CTTTGGCTCC 
CCTGCCGACA GCTGGTGGCG TCCGGAACCC ACCATGCCCC CTACTTCCTG GCGGCAGCTG 
AATCCAGAGA GTATTCGACC AGGGGGACCT GGGGGCCTAT CCCGCTCTTT AGGCCGGGAA 
GAAGAGGAGG ACGAGGAGGA AGAGCTAGAA GAAGGGACCA TAGATGTTAC CGACTTCTTG 
TCCATGACCC AGCAGGATTC CCACGCTCCA CTCAGGGACT CAAGGGGGAG TTCCTTTGAG 
ATGACAGATG ACGACAGTGC CATTAGGGCT CTGACCCAGT TTCCACTTCC CAAGAACCTT 
CTGGCCAAGG TGATTCAGAT TGCAACGTCA TCCTCCACAG CTAAGAACCT CATGCAGTTC 
CATACTGTGG GCACCAAGAC CAAGCTGTCT ACACTCACCC TGCTCTGGCC CTGCCCCATG 
ACCTTTGTTG CCAAAGGGCG CCGCAAAGCA GAGGCTGAGA ATAAGGCGGC AGCCTTGGCC 
TGCAAGAAAC TGAAGAGCCT GGGCCTGGTG GACAGGAACA ACGAACCGCT TACACACGCC 
ATGTATAACC TGGCCTCTTT GCGTGAGCTG GGTGAGACCC AGCGCCGACC ATGCACCATC 
CAGGTGCCCG AGCCCATCCT CCGCAAGATA GAGACCTTCC TGAACCATTA CCCTGTGGAG 
AGTTCATGGA TCGCCCCAGA ACTCCGGCTG CAGAGTGATG ACATCTTGCC CTTGGGCAAG 
GACTCAGGGC CTCTGAGTGA CCCTATCACA GGCAAGCCCT ATGTGCCCCT GTTGGAAGCA 
GAGGAGGTAC GTCTCAGCCA GAGTCTGCTA GAACTGTGGC GGCGGCGAGG GCCGGTCTGG 
CAGGAGGCCC CCCAGCTACC TGTGGACCCA CATCGGGACA CCATCCTCAA CGCCATTGAG 
CAGCACCCGG TGGTGGTCAT CTCTGGGGAC ACGGGCTGTG GGAAGACCAC GCGCATCCCC 
CAGCTGTTGC TGGAGCGCTA TGTGACCGAG GGCCGAGGTG CCCGCTGCAA TGTTATCATC 
ACCCAACCTC GCCGCATCTC TGCTGTGTCT GTGGCACAGC GGGTCAGCCA CGAACTGGGC 
CCCTCCCTGC GCCGGAATGT GGGCTTCCAG GTGCGGTTGG AAAGTAAGCC CCCATCCCGA 
GGCGGGGCCC TGCTCTTCTG CACTGTGGGT ATCCTGCTGC GTAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GCGTGAGCCA CGTCATCGTG GATGAGGTGC ATGAGCGGGA CGTGAACACA 
GACTTTCTGC TGATCCTGCT CAAGGGCCTG CAGCGGCTCA ACCCGGCCCT GCGGCTGGTG 
CTCATGAGTG CCACAGGGGA CAATGAGCGC TTCTCCCGAT ACTTTGGTGG CTGCCCCGTC 
ATCAAGGTGC CTGGCTTCAT GTACCCAGTC AAGGAGCACT ACCTAGAGGA CATCCTGGCC 
AAGTTGGGCA AGCACCAGTA CCTGCACCGG CACCGGCACC ATGAGTCTGA GGATGAATGC 
GCACTCGATT TGGACCTTGT GACTGATCTG GTTCTGCACA TCGATGCTCG CGGGGAACCA 
GGTGGGATCC TGTGCTTCCT GCCTGGGTGG CAGGAGATCA AAGGAGTGCA GCAGCGCCTC 
CAGGAGGCCC TGGGCATGCA CGAGAGCAAG TACCTCATCC TGCCAGTGCA CTCCAACATC 
CCCATGATGG ATCAGAAGGC CATATTCCAG CAGCCTCCAG TTGGGGTGCG CAAGATTGTC 
TTGGCCACCA ACATTGCTGA GACTTCCATC ACAATCAATG ACATCGTGCA TGTGGTGGAC 
AGTGGGCTGC ACAAGGAAGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACA 
GTGTGGGTAT CAAGAGCCAA TGTGATCCAG CGCCGGGGCC GGGCGGGCCG CTGCCAGTCC 
GGCTTTGCCT ACCACTTGTT CCCTCGAAGC CGGCTGGAGA AAATGGTCCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCGAAAAT CCACATGCCT 
GAGAAGACGG CGGTGGAGTT CCTGTCCAAG GCTGTGGACA GTCCAAACAT CAAGGCAGTG 
GACGAGGCTG TGATCTTGCT CCAGGAGATC GGGGTGCTGG ACCAGCGGGA GTACCTGACT 
ACCCTGGGGC AGCGCCTGGC TCACATCTCC ACCGACCCCC GGTTGGCCAA GGCCATTGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTACTGGTGG TCGTTTCCTG CCTCACCCGG 
GACCCCTTCA GCAGCAGCCT ACAGAACCGG GCAGAGGTGG ACAAGGTGAA AGCACTGTTG 
AGCCATGACA GCGGCAGTGA CCACCTGGCC TTTGTGCGGG CTGTCGCCGG CTGGGAGGAG 
GTGCTGCGTT GGCAGGACCG CAGCTCCCGG GAGAATTACC TGGAGGAAAA CCTGCTGTAC 
GCACCCAGCC TGCGCTTCAT CCACGGACTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCCTTCCTGG TGGGGAAGCC CTCGGACTGC ACCCTGGCCT CCGCCCAGTG CAACGAGTAC 
AGTGAGGAGG AGGAGCTGGT GAAGGGCGTG CTGATGGCCG GCCTCTACCC CAACCTCATC 
CAGGTGAGGC AGGGCAAGGT CACCCGGCAG GGGAAGTTCA AGCCCAACAG CGTCACATAT 
AGGACCAAAT CAGGCAACAT CCTGCTGCAC AAGTCGACCA TTAACAGGGA GGCCACACGG 
TTACGGAGCC GATGGCTGAC GTATTTCATG GCAGTCAAGT CCAATGGCAG CGTCTTCGTC 
CGGGACTCCT CTCAGGTGCA CCCGCTAGCT GTGCTGCTGC TGACCGACGG GGACGTGCAC 
ATCCGTGATG ACGGGCGCCG GGCCACCATC TCACTGAGCG ACAGTGACCT GCTGCGGCTG 
GAGGGTGACT CGCGTACCGT GCGGCTGCTG AAGGAGCTGC GGCGGGCCCT GGGCCGCATG 
GTGGAGCGGA GCCTGCGCAG CGAGCTGGCT GCACTTCCCC CCAGCGTACA GGAGGAGCAC 
GGGCAGCTGC TTGCGCTACT GGCAGAGCTG CTGCGAGGAC CCTGTGGCAG CTTTGATGTG 
CGCAAGACAG CTGACGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531799.1
CDS	553..4053
Translation	MCVNPTPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_011533497.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533497.2

ORF Insert Sequence:

ATGTGTGTCA ACCCTACCCC AGGAGGGACC ATCTCTCGAG CTTCTAGGGA CCTATTAAAA 
GAGTTCCCAC AGCCCAAAAA TCTTCTCAAC AGTGTGATTG GAAGAGCCCT CGGCATCTCA 
CATGCAAAAG ACAAACTAGT CTACGTGCAC ACAAATGGAC CGAAGAAAAA GAAAGTCACA 
CTGCACATAA AATGGCCCAA GAGCGTGGAG GTAGAAGGCT ATGGCAGCAA GAAGATCGAT 
GCTGAGCGGC AGGCTGCAGC TGCAGCCTGC CAGCTGTTCA AGGGTTGGGG TCTGCTAGGT 
CCCCGGAATG AGTTGTTTGA CGCAGCCAAA TACCGAGTGC TAGCTGATCG CTTTGGCTCC 
CCTGCCGACA GCTGGTGGCG TCCGGAACCC ACCATGCCCC CTACTTCCTG GCGGCAGCTG 
AATCCAGAGA GTATTCGACC AGGGGGACCT GGGGGCCTAT CCCGCTCTTT AGGCCGGGAA 
GAAGAGGAGG ACGAGGAGGA AGAGCTAGAA GAAGGGACCA TAGATGTTAC CGACTTCTTG 
TCCATGACCC AGCAGGATTC CCACGCTCCA CTCAGGGACT CAAGGGGGAG TTCCTTTGAG 
ATGACAGATG ACGACAGTGC CATTAGGGCT CTGACCCAGT TTCCACTTCC CAAGAACCTT 
CTGGCCAAGG TGATTCAGAT TGCAACGTCA TCCTCCACAG CTAAGAACCT CATGCAGTTC 
CATACTGTGG GCACCAAGAC CAAGCTGTCT ACACTCACCC TGCTCTGGCC CTGCCCCATG 
ACCTTTGTTG CCAAAGGGCG CCGCAAAGCA GAGGCTGAGA ATAAGGCGGC AGCCTTGGCC 
TGCAAGAAAC TGAAGAGCCT GGGCCTGGTG GACAGGAACA ACGAACCGCT TACACACGCC 
ATGTATAACC TGGCCTCTTT GCGTGAGCTG GGTGAGACCC AGCGCCGACC ATGCACCATC 
CAGGTGCCCG AGCCCATCCT CCGCAAGATA GAGACCTTCC TGAACCATTA CCCTGTGGAG 
AGTTCATGGA TCGCCCCAGA ACTCCGGCTG CAGAGTGATG ACATCTTGCC CTTGGGCAAG 
GACTCAGGGC CTCTGAGTGA CCCTATCACA GGCAAGCCCT ATGTGCCCCT GTTGGAAGCA 
GAGGAGGTAC GTCTCAGCCA GAGTCTGCTA GAACTGTGGC GGCGGCGAGG GCCGGTCTGG 
CAGGAGGCCC CCCAGCTACC TGTGGACCCA CATCGGGACA CCATCCTCAA CGCCATTGAG 
CAGCACCCGG TGGTGGTCAT CTCTGGGGAC ACGGGCTGTG GGAAGACCAC GCGCATCCCC 
CAGCTGTTGC TGGAGCGCTA TGTGACCGAG GGCCGAGGTG CCCGCTGCAA TGTTATCATC 
ACCCAACCTC GCCGCATCTC TGCTGTGTCT GTGGCACAGC GGGTCAGCCA CGAACTGGGC 
CCCTCCCTGC GCCGGAATGT GGGCTTCCAG GTGCGGTTGG AAAGTAAGCC CCCATCCCGA 
GGCGGGGCCC TGCTCTTCTG CACTGTGGGT ATCCTGCTGC GTAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GCGTGAGCCA CGTCATCGTG GATGAGGTGC ATGAGCGGGA CGTGAACACA 
GACTTTCTGC TGATCCTGCT CAAGGGCCTG CAGCGGCTCA ACCCGGCCCT GCGGCTGGTG 
CTCATGAGTG CCACAGGGGA CAATGAGCGC TTCTCCCGAT ACTTTGGTGG CTGCCCCGTC 
ATCAAGGTGC CTGGCTTCAT GTACCCAGTC AAGGAGCACT ACCTAGAGGA CATCCTGGCC 
AAGTTGGGCA AGCACCAGTA CCTGCACCGG CACCGGCACC ATGAGTCTGA GGATGAATGC 
GCACTCGATT TGGACCTTGT GACTGATCTG GTTCTGCACA TCGATGCTCG CGGGGAACCA 
GGTGGGATCC TGTGCTTCCT GCCTGGGTGG CAGGAGATCA AAGGAGTGCA GCAGCGCCTC 
CAGGAGGCCC TGGGCATGCA CGAGAGCAAG TACCTCATCC TGCCAGTGCA CTCCAACATC 
CCCATGATGG ATCAGAAGGC CATATTCCAG CAGCCTCCAG TTGGGGTGCG CAAGATTGTC 
TTGGCCACCA ACATTGCTGA GACTTCCATC ACAATCAATG ACATCGTGCA TGTGGTGGAC 
AGTGGGCTGC ACAAGGAAGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACA 
GTGTGGGTAT CAAGAGCCAA TGTGATCCAG CGCCGGGGCC GGGCGGGCCG CTGCCAGTCC 
GGCTTTGCCT ACCACTTGTT CCCTCGAAGC CGGCTGGAGA AAATGGTCCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCGAAAAT CCACATGCCT 
GAGAAGACGG CGGTGGAGTT CCTGTCCAAG GCTGTGGACA GTCCAAACAT CAAGGCAGTG 
GACGAGGCTG TGATCTTGCT CCAGGAGATC GGGGTGCTGG ACCAGCGGGA GTACCTGACT 
ACCCTGGGGC AGCGCCTGGC TCACATCTCC ACCGACCCCC GGTTGGCCAA GGCCATTGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTACTGGTGG TCGTTTCCTG CCTCACCCGG 
GACCCCTTCA GCAGCAGCCT ACAGAACCGG GCAGAGGTGG ACAAGGTGAA AGCACTGTTG 
AGCCATGACA GCGGCAGTGA CCACCTGGCC TTTGTGCGGG CTGTCGCCGG CTGGGAGGAG 
GTGCTGCGTT GGCAGGACCG CAGCTCCCGG GAGAATTACC TGGAGGAAAA CCTGCTGTAC 
GCACCCAGCC TGCGCTTCAT CCACGGACTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCCTTCCTGG TGGGGAAGCC CTCGGACTGC ACCCTGGCCT CCGCCCAGTG CAACGAGTAC 
AGTGAGGAGG AGGAGCTGGT GAAGGGCGTG CTGATGGCCG GCCTCTACCC CAACCTCATC 
CAGGTGAGGC AGGGCAAGGT CACCCGGCAG GGGAAGTTCA AGCCCAACAG CGTCACATAT 
AGGACCAAAT CAGGCAACAT CCTGCTGCAC AAGTCGACCA TTAACAGGGA GGCCACACGG 
TTACGGAGCC GATGGCTGAC GTATTTCATG GCAGTCAAGT CCAATGGCAG CGTCTTCGTC 
CGGGACTCCT CTCAGGTGCA CCCGCTAGCT GTGCTGCTGC TGACCGACGG GGACGTGCAC 
ATCCGTGATG ACGGGCGCCG GGCCACCATC TCACTGAGCG ACAGTGACCT GCTGCGGCTG 
GAGGGTGACT CGCGTACCGT GCGGCTGCTG AAGGAGCTGC GGCGGGCCCT GGGCCGCATG 
GTGGAGCGGA GCCTGCGCAG CGAGCTGGCT GCACTTCCCC CCAGCGTACA GGAGGAGCAC 
GGGCAGCTGC TTGCGCTACT GGCAGAGCTG CTGCGAGGAC CCTGTGGCAG CTTTGATGTG 
CGCAAGACAG CTGACGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_011533498.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531800.1 Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X7, mRNA.

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CloneID	OHu65127
Clone ID Related Accession (Same CDS sequence)	XM_011533497.2 , XM_011533498.1 , XM_011533496.1 , NM_001330990.1
Accession Version	XM_011533498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX30 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTCA ACCCTACCCC AGGAGGGACC ATCTCTCGAG CTTCTAGGGA CCTATTAAAA 
GAGTTCCCAC AGCCCAAAAA TCTTCTCAAC AGTGTGATTG GAAGAGCCCT CGGCATCTCA 
CATGCAAAAG ACAAACTAGT CTACGTGCAC ACAAATGGAC CGAAGAAAAA GAAAGTCACA 
CTGCACATAA AATGGCCCAA GAGCGTGGAG GTAGAAGGCT ATGGCAGCAA GAAGATCGAT 
GCTGAGCGGC AGGCTGCAGC TGCAGCCTGC CAGCTGTTCA AGGGTTGGGG TCTGCTAGGT 
CCCCGGAATG AGTTGTTTGA CGCAGCCAAA TACCGAGTGC TAGCTGATCG CTTTGGCTCC 
CCTGCCGACA GCTGGTGGCG TCCGGAACCC ACCATGCCCC CTACTTCCTG GCGGCAGCTG 
AATCCAGAGA GTATTCGACC AGGGGGACCT GGGGGCCTAT CCCGCTCTTT AGGCCGGGAA 
GAAGAGGAGG ACGAGGAGGA AGAGCTAGAA GAAGGGACCA TAGATGTTAC CGACTTCTTG 
TCCATGACCC AGCAGGATTC CCACGCTCCA CTCAGGGACT CAAGGGGGAG TTCCTTTGAG 
ATGACAGATG ACGACAGTGC CATTAGGGCT CTGACCCAGT TTCCACTTCC CAAGAACCTT 
CTGGCCAAGG TGATTCAGAT TGCAACGTCA TCCTCCACAG CTAAGAACCT CATGCAGTTC 
CATACTGTGG GCACCAAGAC CAAGCTGTCT ACACTCACCC TGCTCTGGCC CTGCCCCATG 
ACCTTTGTTG CCAAAGGGCG CCGCAAAGCA GAGGCTGAGA ATAAGGCGGC AGCCTTGGCC 
TGCAAGAAAC TGAAGAGCCT GGGCCTGGTG GACAGGAACA ACGAACCGCT TACACACGCC 
ATGTATAACC TGGCCTCTTT GCGTGAGCTG GGTGAGACCC AGCGCCGACC ATGCACCATC 
CAGGTGCCCG AGCCCATCCT CCGCAAGATA GAGACCTTCC TGAACCATTA CCCTGTGGAG 
AGTTCATGGA TCGCCCCAGA ACTCCGGCTG CAGAGTGATG ACATCTTGCC CTTGGGCAAG 
GACTCAGGGC CTCTGAGTGA CCCTATCACA GGCAAGCCCT ATGTGCCCCT GTTGGAAGCA 
GAGGAGGTAC GTCTCAGCCA GAGTCTGCTA GAACTGTGGC GGCGGCGAGG GCCGGTCTGG 
CAGGAGGCCC CCCAGCTACC TGTGGACCCA CATCGGGACA CCATCCTCAA CGCCATTGAG 
CAGCACCCGG TGGTGGTCAT CTCTGGGGAC ACGGGCTGTG GGAAGACCAC GCGCATCCCC 
CAGCTGTTGC TGGAGCGCTA TGTGACCGAG GGCCGAGGTG CCCGCTGCAA TGTTATCATC 
ACCCAACCTC GCCGCATCTC TGCTGTGTCT GTGGCACAGC GGGTCAGCCA CGAACTGGGC 
CCCTCCCTGC GCCGGAATGT GGGCTTCCAG GTGCGGTTGG AAAGTAAGCC CCCATCCCGA 
GGCGGGGCCC TGCTCTTCTG CACTGTGGGT ATCCTGCTGC GTAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GCGTGAGCCA CGTCATCGTG GATGAGGTGC ATGAGCGGGA CGTGAACACA 
GACTTTCTGC TGATCCTGCT CAAGGGCCTG CAGCGGCTCA ACCCGGCCCT GCGGCTGGTG 
CTCATGAGTG CCACAGGGGA CAATGAGCGC TTCTCCCGAT ACTTTGGTGG CTGCCCCGTC 
ATCAAGGTGC CTGGCTTCAT GTACCCAGTC AAGGAGCACT ACCTAGAGGA CATCCTGGCC 
AAGTTGGGCA AGCACCAGTA CCTGCACCGG CACCGGCACC ATGAGTCTGA GGATGAATGC 
GCACTCGATT TGGACCTTGT GACTGATCTG GTTCTGCACA TCGATGCTCG CGGGGAACCA 
GGTGGGATCC TGTGCTTCCT GCCTGGGTGG CAGGAGATCA AAGGAGTGCA GCAGCGCCTC 
CAGGAGGCCC TGGGCATGCA CGAGAGCAAG TACCTCATCC TGCCAGTGCA CTCCAACATC 
CCCATGATGG ATCAGAAGGC CATATTCCAG CAGCCTCCAG TTGGGGTGCG CAAGATTGTC 
TTGGCCACCA ACATTGCTGA GACTTCCATC ACAATCAATG ACATCGTGCA TGTGGTGGAC 
AGTGGGCTGC ACAAGGAAGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACA 
GTGTGGGTAT CAAGAGCCAA TGTGATCCAG CGCCGGGGCC GGGCGGGCCG CTGCCAGTCC 
GGCTTTGCCT ACCACTTGTT CCCTCGAAGC CGGCTGGAGA AAATGGTCCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCGAAAAT CCACATGCCT 
GAGAAGACGG CGGTGGAGTT CCTGTCCAAG GCTGTGGACA GTCCAAACAT CAAGGCAGTG 
GACGAGGCTG TGATCTTGCT CCAGGAGATC GGGGTGCTGG ACCAGCGGGA GTACCTGACT 
ACCCTGGGGC AGCGCCTGGC TCACATCTCC ACCGACCCCC GGTTGGCCAA GGCCATTGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTACTGGTGG TCGTTTCCTG CCTCACCCGG 
GACCCCTTCA GCAGCAGCCT ACAGAACCGG GCAGAGGTGG ACAAGGTGAA AGCACTGTTG 
AGCCATGACA GCGGCAGTGA CCACCTGGCC TTTGTGCGGG CTGTCGCCGG CTGGGAGGAG 
GTGCTGCGTT GGCAGGACCG CAGCTCCCGG GAGAATTACC TGGAGGAAAA CCTGCTGTAC 
GCACCCAGCC TGCGCTTCAT CCACGGACTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCCTTCCTGG TGGGGAAGCC CTCGGACTGC ACCCTGGCCT CCGCCCAGTG CAACGAGTAC 
AGTGAGGAGG AGGAGCTGGT GAAGGGCGTG CTGATGGCCG GCCTCTACCC CAACCTCATC 
CAGGTGAGGC AGGGCAAGGT CACCCGGCAG GGGAAGTTCA AGCCCAACAG CGTCACATAT 
AGGACCAAAT CAGGCAACAT CCTGCTGCAC AAGTCGACCA TTAACAGGGA GGCCACACGG 
TTACGGAGCC GATGGCTGAC GTATTTCATG GCAGTCAAGT CCAATGGCAG CGTCTTCGTC 
CGGGACTCCT CTCAGGTGCA CCCGCTAGCT GTGCTGCTGC TGACCGACGG GGACGTGCAC 
ATCCGTGATG ACGGGCGCCG GGCCACCATC TCACTGAGCG ACAGTGACCT GCTGCGGCTG 
GAGGGTGACT CGCGTACCGT GCGGCTGCTG AAGGAGCTGC GGCGGGCCCT GGGCCGCATG 
GTGGAGCGGA GCCTGCGCAG CGAGCTGGCT GCACTTCCCC CCAGCGTACA GGAGGAGCAC 
GGGCAGCTGC TTGCGCTACT GGCAGAGCTG CTGCGAGGAC CCTGTGGCAG CTTTGATGTG 
CGCAAGACAG CTGACGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531800.1
CDS	623..4123
Translation	MCVNPTPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_011533498.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533498.1

ORF Insert Sequence:

ATGTGTGTCA ACCCTACCCC AGGAGGGACC ATCTCTCGAG CTTCTAGGGA CCTATTAAAA 
GAGTTCCCAC AGCCCAAAAA TCTTCTCAAC AGTGTGATTG GAAGAGCCCT CGGCATCTCA 
CATGCAAAAG ACAAACTAGT CTACGTGCAC ACAAATGGAC CGAAGAAAAA GAAAGTCACA 
CTGCACATAA AATGGCCCAA GAGCGTGGAG GTAGAAGGCT ATGGCAGCAA GAAGATCGAT 
GCTGAGCGGC AGGCTGCAGC TGCAGCCTGC CAGCTGTTCA AGGGTTGGGG TCTGCTAGGT 
CCCCGGAATG AGTTGTTTGA CGCAGCCAAA TACCGAGTGC TAGCTGATCG CTTTGGCTCC 
CCTGCCGACA GCTGGTGGCG TCCGGAACCC ACCATGCCCC CTACTTCCTG GCGGCAGCTG 
AATCCAGAGA GTATTCGACC AGGGGGACCT GGGGGCCTAT CCCGCTCTTT AGGCCGGGAA 
GAAGAGGAGG ACGAGGAGGA AGAGCTAGAA GAAGGGACCA TAGATGTTAC CGACTTCTTG 
TCCATGACCC AGCAGGATTC CCACGCTCCA CTCAGGGACT CAAGGGGGAG TTCCTTTGAG 
ATGACAGATG ACGACAGTGC CATTAGGGCT CTGACCCAGT TTCCACTTCC CAAGAACCTT 
CTGGCCAAGG TGATTCAGAT TGCAACGTCA TCCTCCACAG CTAAGAACCT CATGCAGTTC 
CATACTGTGG GCACCAAGAC CAAGCTGTCT ACACTCACCC TGCTCTGGCC CTGCCCCATG 
ACCTTTGTTG CCAAAGGGCG CCGCAAAGCA GAGGCTGAGA ATAAGGCGGC AGCCTTGGCC 
TGCAAGAAAC TGAAGAGCCT GGGCCTGGTG GACAGGAACA ACGAACCGCT TACACACGCC 
ATGTATAACC TGGCCTCTTT GCGTGAGCTG GGTGAGACCC AGCGCCGACC ATGCACCATC 
CAGGTGCCCG AGCCCATCCT CCGCAAGATA GAGACCTTCC TGAACCATTA CCCTGTGGAG 
AGTTCATGGA TCGCCCCAGA ACTCCGGCTG CAGAGTGATG ACATCTTGCC CTTGGGCAAG 
GACTCAGGGC CTCTGAGTGA CCCTATCACA GGCAAGCCCT ATGTGCCCCT GTTGGAAGCA 
GAGGAGGTAC GTCTCAGCCA GAGTCTGCTA GAACTGTGGC GGCGGCGAGG GCCGGTCTGG 
CAGGAGGCCC CCCAGCTACC TGTGGACCCA CATCGGGACA CCATCCTCAA CGCCATTGAG 
CAGCACCCGG TGGTGGTCAT CTCTGGGGAC ACGGGCTGTG GGAAGACCAC GCGCATCCCC 
CAGCTGTTGC TGGAGCGCTA TGTGACCGAG GGCCGAGGTG CCCGCTGCAA TGTTATCATC 
ACCCAACCTC GCCGCATCTC TGCTGTGTCT GTGGCACAGC GGGTCAGCCA CGAACTGGGC 
CCCTCCCTGC GCCGGAATGT GGGCTTCCAG GTGCGGTTGG AAAGTAAGCC CCCATCCCGA 
GGCGGGGCCC TGCTCTTCTG CACTGTGGGT ATCCTGCTGC GTAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GCGTGAGCCA CGTCATCGTG GATGAGGTGC ATGAGCGGGA CGTGAACACA 
GACTTTCTGC TGATCCTGCT CAAGGGCCTG CAGCGGCTCA ACCCGGCCCT GCGGCTGGTG 
CTCATGAGTG CCACAGGGGA CAATGAGCGC TTCTCCCGAT ACTTTGGTGG CTGCCCCGTC 
ATCAAGGTGC CTGGCTTCAT GTACCCAGTC AAGGAGCACT ACCTAGAGGA CATCCTGGCC 
AAGTTGGGCA AGCACCAGTA CCTGCACCGG CACCGGCACC ATGAGTCTGA GGATGAATGC 
GCACTCGATT TGGACCTTGT GACTGATCTG GTTCTGCACA TCGATGCTCG CGGGGAACCA 
GGTGGGATCC TGTGCTTCCT GCCTGGGTGG CAGGAGATCA AAGGAGTGCA GCAGCGCCTC 
CAGGAGGCCC TGGGCATGCA CGAGAGCAAG TACCTCATCC TGCCAGTGCA CTCCAACATC 
CCCATGATGG ATCAGAAGGC CATATTCCAG CAGCCTCCAG TTGGGGTGCG CAAGATTGTC 
TTGGCCACCA ACATTGCTGA GACTTCCATC ACAATCAATG ACATCGTGCA TGTGGTGGAC 
AGTGGGCTGC ACAAGGAAGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACA 
GTGTGGGTAT CAAGAGCCAA TGTGATCCAG CGCCGGGGCC GGGCGGGCCG CTGCCAGTCC 
GGCTTTGCCT ACCACTTGTT CCCTCGAAGC CGGCTGGAGA AAATGGTCCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCGAAAAT CCACATGCCT 
GAGAAGACGG CGGTGGAGTT CCTGTCCAAG GCTGTGGACA GTCCAAACAT CAAGGCAGTG 
GACGAGGCTG TGATCTTGCT CCAGGAGATC GGGGTGCTGG ACCAGCGGGA GTACCTGACT 
ACCCTGGGGC AGCGCCTGGC TCACATCTCC ACCGACCCCC GGTTGGCCAA GGCCATTGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTACTGGTGG TCGTTTCCTG CCTCACCCGG 
GACCCCTTCA GCAGCAGCCT ACAGAACCGG GCAGAGGTGG ACAAGGTGAA AGCACTGTTG 
AGCCATGACA GCGGCAGTGA CCACCTGGCC TTTGTGCGGG CTGTCGCCGG CTGGGAGGAG 
GTGCTGCGTT GGCAGGACCG CAGCTCCCGG GAGAATTACC TGGAGGAAAA CCTGCTGTAC 
GCACCCAGCC TGCGCTTCAT CCACGGACTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCCTTCCTGG TGGGGAAGCC CTCGGACTGC ACCCTGGCCT CCGCCCAGTG CAACGAGTAC 
AGTGAGGAGG AGGAGCTGGT GAAGGGCGTG CTGATGGCCG GCCTCTACCC CAACCTCATC 
CAGGTGAGGC AGGGCAAGGT CACCCGGCAG GGGAAGTTCA AGCCCAACAG CGTCACATAT 
AGGACCAAAT CAGGCAACAT CCTGCTGCAC AAGTCGACCA TTAACAGGGA GGCCACACGG 
TTACGGAGCC GATGGCTGAC GTATTTCATG GCAGTCAAGT CCAATGGCAG CGTCTTCGTC 
CGGGACTCCT CTCAGGTGCA CCCGCTAGCT GTGCTGCTGC TGACCGACGG GGACGTGCAC 
ATCCGTGATG ACGGGCGCCG GGCCACCATC TCACTGAGCG ACAGTGACCT GCTGCGGCTG 
GAGGGTGACT CGCGTACCGT GCGGCTGCTG AAGGAGCTGC GGCGGGCCCT GGGCCGCATG 
GTGGAGCGGA GCCTGCGCAG CGAGCTGGCT GCACTTCCCC CCAGCGTACA GGAGGAGCAC 
GGGCAGCTGC TTGCGCTACT GGCAGAGCTG CTGCGAGGAC CCTGTGGCAG CTTTGATGTG 
CGCAAGACAG CTGACGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_011533496.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531798.1 Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X8, mRNA.

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CloneID	OHu65127
Clone ID Related Accession (Same CDS sequence)	XM_011533497.2 , XM_011533498.1 , XM_011533496.1 , NM_001330990.1
Accession Version	XM_011533496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative ATP-dependent RNA helicase DHX30 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTCA ACCCTACCCC AGGAGGGACC ATCTCTCGAG CTTCTAGGGA CCTATTAAAA 
GAGTTCCCAC AGCCCAAAAA TCTTCTCAAC AGTGTGATTG GAAGAGCCCT CGGCATCTCA 
CATGCAAAAG ACAAACTAGT CTACGTGCAC ACAAATGGAC CGAAGAAAAA GAAAGTCACA 
CTGCACATAA AATGGCCCAA GAGCGTGGAG GTAGAAGGCT ATGGCAGCAA GAAGATCGAT 
GCTGAGCGGC AGGCTGCAGC TGCAGCCTGC CAGCTGTTCA AGGGTTGGGG TCTGCTAGGT 
CCCCGGAATG AGTTGTTTGA CGCAGCCAAA TACCGAGTGC TAGCTGATCG CTTTGGCTCC 
CCTGCCGACA GCTGGTGGCG TCCGGAACCC ACCATGCCCC CTACTTCCTG GCGGCAGCTG 
AATCCAGAGA GTATTCGACC AGGGGGACCT GGGGGCCTAT CCCGCTCTTT AGGCCGGGAA 
GAAGAGGAGG ACGAGGAGGA AGAGCTAGAA GAAGGGACCA TAGATGTTAC CGACTTCTTG 
TCCATGACCC AGCAGGATTC CCACGCTCCA CTCAGGGACT CAAGGGGGAG TTCCTTTGAG 
ATGACAGATG ACGACAGTGC CATTAGGGCT CTGACCCAGT TTCCACTTCC CAAGAACCTT 
CTGGCCAAGG TGATTCAGAT TGCAACGTCA TCCTCCACAG CTAAGAACCT CATGCAGTTC 
CATACTGTGG GCACCAAGAC CAAGCTGTCT ACACTCACCC TGCTCTGGCC CTGCCCCATG 
ACCTTTGTTG CCAAAGGGCG CCGCAAAGCA GAGGCTGAGA ATAAGGCGGC AGCCTTGGCC 
TGCAAGAAAC TGAAGAGCCT GGGCCTGGTG GACAGGAACA ACGAACCGCT TACACACGCC 
ATGTATAACC TGGCCTCTTT GCGTGAGCTG GGTGAGACCC AGCGCCGACC ATGCACCATC 
CAGGTGCCCG AGCCCATCCT CCGCAAGATA GAGACCTTCC TGAACCATTA CCCTGTGGAG 
AGTTCATGGA TCGCCCCAGA ACTCCGGCTG CAGAGTGATG ACATCTTGCC CTTGGGCAAG 
GACTCAGGGC CTCTGAGTGA CCCTATCACA GGCAAGCCCT ATGTGCCCCT GTTGGAAGCA 
GAGGAGGTAC GTCTCAGCCA GAGTCTGCTA GAACTGTGGC GGCGGCGAGG GCCGGTCTGG 
CAGGAGGCCC CCCAGCTACC TGTGGACCCA CATCGGGACA CCATCCTCAA CGCCATTGAG 
CAGCACCCGG TGGTGGTCAT CTCTGGGGAC ACGGGCTGTG GGAAGACCAC GCGCATCCCC 
CAGCTGTTGC TGGAGCGCTA TGTGACCGAG GGCCGAGGTG CCCGCTGCAA TGTTATCATC 
ACCCAACCTC GCCGCATCTC TGCTGTGTCT GTGGCACAGC GGGTCAGCCA CGAACTGGGC 
CCCTCCCTGC GCCGGAATGT GGGCTTCCAG GTGCGGTTGG AAAGTAAGCC CCCATCCCGA 
GGCGGGGCCC TGCTCTTCTG CACTGTGGGT ATCCTGCTGC GTAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GCGTGAGCCA CGTCATCGTG GATGAGGTGC ATGAGCGGGA CGTGAACACA 
GACTTTCTGC TGATCCTGCT CAAGGGCCTG CAGCGGCTCA ACCCGGCCCT GCGGCTGGTG 
CTCATGAGTG CCACAGGGGA CAATGAGCGC TTCTCCCGAT ACTTTGGTGG CTGCCCCGTC 
ATCAAGGTGC CTGGCTTCAT GTACCCAGTC AAGGAGCACT ACCTAGAGGA CATCCTGGCC 
AAGTTGGGCA AGCACCAGTA CCTGCACCGG CACCGGCACC ATGAGTCTGA GGATGAATGC 
GCACTCGATT TGGACCTTGT GACTGATCTG GTTCTGCACA TCGATGCTCG CGGGGAACCA 
GGTGGGATCC TGTGCTTCCT GCCTGGGTGG CAGGAGATCA AAGGAGTGCA GCAGCGCCTC 
CAGGAGGCCC TGGGCATGCA CGAGAGCAAG TACCTCATCC TGCCAGTGCA CTCCAACATC 
CCCATGATGG ATCAGAAGGC CATATTCCAG CAGCCTCCAG TTGGGGTGCG CAAGATTGTC 
TTGGCCACCA ACATTGCTGA GACTTCCATC ACAATCAATG ACATCGTGCA TGTGGTGGAC 
AGTGGGCTGC ACAAGGAAGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACA 
GTGTGGGTAT CAAGAGCCAA TGTGATCCAG CGCCGGGGCC GGGCGGGCCG CTGCCAGTCC 
GGCTTTGCCT ACCACTTGTT CCCTCGAAGC CGGCTGGAGA AAATGGTCCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCGAAAAT CCACATGCCT 
GAGAAGACGG CGGTGGAGTT CCTGTCCAAG GCTGTGGACA GTCCAAACAT CAAGGCAGTG 
GACGAGGCTG TGATCTTGCT CCAGGAGATC GGGGTGCTGG ACCAGCGGGA GTACCTGACT 
ACCCTGGGGC AGCGCCTGGC TCACATCTCC ACCGACCCCC GGTTGGCCAA GGCCATTGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTACTGGTGG TCGTTTCCTG CCTCACCCGG 
GACCCCTTCA GCAGCAGCCT ACAGAACCGG GCAGAGGTGG ACAAGGTGAA AGCACTGTTG 
AGCCATGACA GCGGCAGTGA CCACCTGGCC TTTGTGCGGG CTGTCGCCGG CTGGGAGGAG 
GTGCTGCGTT GGCAGGACCG CAGCTCCCGG GAGAATTACC TGGAGGAAAA CCTGCTGTAC 
GCACCCAGCC TGCGCTTCAT CCACGGACTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCCTTCCTGG TGGGGAAGCC CTCGGACTGC ACCCTGGCCT CCGCCCAGTG CAACGAGTAC 
AGTGAGGAGG AGGAGCTGGT GAAGGGCGTG CTGATGGCCG GCCTCTACCC CAACCTCATC 
CAGGTGAGGC AGGGCAAGGT CACCCGGCAG GGGAAGTTCA AGCCCAACAG CGTCACATAT 
AGGACCAAAT CAGGCAACAT CCTGCTGCAC AAGTCGACCA TTAACAGGGA GGCCACACGG 
TTACGGAGCC GATGGCTGAC GTATTTCATG GCAGTCAAGT CCAATGGCAG CGTCTTCGTC 
CGGGACTCCT CTCAGGTGCA CCCGCTAGCT GTGCTGCTGC TGACCGACGG GGACGTGCAC 
ATCCGTGATG ACGGGCGCCG GGCCACCATC TCACTGAGCG ACAGTGACCT GCTGCGGCTG 
GAGGGTGACT CGCGTACCGT GCGGCTGCTG AAGGAGCTGC GGCGGGCCCT GGGCCGCATG 
GTGGAGCGGA GCCTGCGCAG CGAGCTGGCT GCACTTCCCC CCAGCGTACA GGAGGAGCAC 
GGGCAGCTGC TTGCGCTACT GGCAGAGCTG CTGCGAGGAC CCTGTGGCAG CTTTGATGTG 
CGCAAGACAG CTGACGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531798.1
CDS	672..4172
Translation	MCVNPTPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_011533496.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533496.1

ORF Insert Sequence:

ATGTGTGTCA ACCCTACCCC AGGAGGGACC ATCTCTCGAG CTTCTAGGGA CCTATTAAAA 
GAGTTCCCAC AGCCCAAAAA TCTTCTCAAC AGTGTGATTG GAAGAGCCCT CGGCATCTCA 
CATGCAAAAG ACAAACTAGT CTACGTGCAC ACAAATGGAC CGAAGAAAAA GAAAGTCACA 
CTGCACATAA AATGGCCCAA GAGCGTGGAG GTAGAAGGCT ATGGCAGCAA GAAGATCGAT 
GCTGAGCGGC AGGCTGCAGC TGCAGCCTGC CAGCTGTTCA AGGGTTGGGG TCTGCTAGGT 
CCCCGGAATG AGTTGTTTGA CGCAGCCAAA TACCGAGTGC TAGCTGATCG CTTTGGCTCC 
CCTGCCGACA GCTGGTGGCG TCCGGAACCC ACCATGCCCC CTACTTCCTG GCGGCAGCTG 
AATCCAGAGA GTATTCGACC AGGGGGACCT GGGGGCCTAT CCCGCTCTTT AGGCCGGGAA 
GAAGAGGAGG ACGAGGAGGA AGAGCTAGAA GAAGGGACCA TAGATGTTAC CGACTTCTTG 
TCCATGACCC AGCAGGATTC CCACGCTCCA CTCAGGGACT CAAGGGGGAG TTCCTTTGAG 
ATGACAGATG ACGACAGTGC CATTAGGGCT CTGACCCAGT TTCCACTTCC CAAGAACCTT 
CTGGCCAAGG TGATTCAGAT TGCAACGTCA TCCTCCACAG CTAAGAACCT CATGCAGTTC 
CATACTGTGG GCACCAAGAC CAAGCTGTCT ACACTCACCC TGCTCTGGCC CTGCCCCATG 
ACCTTTGTTG CCAAAGGGCG CCGCAAAGCA GAGGCTGAGA ATAAGGCGGC AGCCTTGGCC 
TGCAAGAAAC TGAAGAGCCT GGGCCTGGTG GACAGGAACA ACGAACCGCT TACACACGCC 
ATGTATAACC TGGCCTCTTT GCGTGAGCTG GGTGAGACCC AGCGCCGACC ATGCACCATC 
CAGGTGCCCG AGCCCATCCT CCGCAAGATA GAGACCTTCC TGAACCATTA CCCTGTGGAG 
AGTTCATGGA TCGCCCCAGA ACTCCGGCTG CAGAGTGATG ACATCTTGCC CTTGGGCAAG 
GACTCAGGGC CTCTGAGTGA CCCTATCACA GGCAAGCCCT ATGTGCCCCT GTTGGAAGCA 
GAGGAGGTAC GTCTCAGCCA GAGTCTGCTA GAACTGTGGC GGCGGCGAGG GCCGGTCTGG 
CAGGAGGCCC CCCAGCTACC TGTGGACCCA CATCGGGACA CCATCCTCAA CGCCATTGAG 
CAGCACCCGG TGGTGGTCAT CTCTGGGGAC ACGGGCTGTG GGAAGACCAC GCGCATCCCC 
CAGCTGTTGC TGGAGCGCTA TGTGACCGAG GGCCGAGGTG CCCGCTGCAA TGTTATCATC 
ACCCAACCTC GCCGCATCTC TGCTGTGTCT GTGGCACAGC GGGTCAGCCA CGAACTGGGC 
CCCTCCCTGC GCCGGAATGT GGGCTTCCAG GTGCGGTTGG AAAGTAAGCC CCCATCCCGA 
GGCGGGGCCC TGCTCTTCTG CACTGTGGGT ATCCTGCTGC GTAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GCGTGAGCCA CGTCATCGTG GATGAGGTGC ATGAGCGGGA CGTGAACACA 
GACTTTCTGC TGATCCTGCT CAAGGGCCTG CAGCGGCTCA ACCCGGCCCT GCGGCTGGTG 
CTCATGAGTG CCACAGGGGA CAATGAGCGC TTCTCCCGAT ACTTTGGTGG CTGCCCCGTC 
ATCAAGGTGC CTGGCTTCAT GTACCCAGTC AAGGAGCACT ACCTAGAGGA CATCCTGGCC 
AAGTTGGGCA AGCACCAGTA CCTGCACCGG CACCGGCACC ATGAGTCTGA GGATGAATGC 
GCACTCGATT TGGACCTTGT GACTGATCTG GTTCTGCACA TCGATGCTCG CGGGGAACCA 
GGTGGGATCC TGTGCTTCCT GCCTGGGTGG CAGGAGATCA AAGGAGTGCA GCAGCGCCTC 
CAGGAGGCCC TGGGCATGCA CGAGAGCAAG TACCTCATCC TGCCAGTGCA CTCCAACATC 
CCCATGATGG ATCAGAAGGC CATATTCCAG CAGCCTCCAG TTGGGGTGCG CAAGATTGTC 
TTGGCCACCA ACATTGCTGA GACTTCCATC ACAATCAATG ACATCGTGCA TGTGGTGGAC 
AGTGGGCTGC ACAAGGAAGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACA 
GTGTGGGTAT CAAGAGCCAA TGTGATCCAG CGCCGGGGCC GGGCGGGCCG CTGCCAGTCC 
GGCTTTGCCT ACCACTTGTT CCCTCGAAGC CGGCTGGAGA AAATGGTCCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCGAAAAT CCACATGCCT 
GAGAAGACGG CGGTGGAGTT CCTGTCCAAG GCTGTGGACA GTCCAAACAT CAAGGCAGTG 
GACGAGGCTG TGATCTTGCT CCAGGAGATC GGGGTGCTGG ACCAGCGGGA GTACCTGACT 
ACCCTGGGGC AGCGCCTGGC TCACATCTCC ACCGACCCCC GGTTGGCCAA GGCCATTGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTACTGGTGG TCGTTTCCTG CCTCACCCGG 
GACCCCTTCA GCAGCAGCCT ACAGAACCGG GCAGAGGTGG ACAAGGTGAA AGCACTGTTG 
AGCCATGACA GCGGCAGTGA CCACCTGGCC TTTGTGCGGG CTGTCGCCGG CTGGGAGGAG 
GTGCTGCGTT GGCAGGACCG CAGCTCCCGG GAGAATTACC TGGAGGAAAA CCTGCTGTAC 
GCACCCAGCC TGCGCTTCAT CCACGGACTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCCTTCCTGG TGGGGAAGCC CTCGGACTGC ACCCTGGCCT CCGCCCAGTG CAACGAGTAC 
AGTGAGGAGG AGGAGCTGGT GAAGGGCGTG CTGATGGCCG GCCTCTACCC CAACCTCATC 
CAGGTGAGGC AGGGCAAGGT CACCCGGCAG GGGAAGTTCA AGCCCAACAG CGTCACATAT 
AGGACCAAAT CAGGCAACAT CCTGCTGCAC AAGTCGACCA TTAACAGGGA GGCCACACGG 
TTACGGAGCC GATGGCTGAC GTATTTCATG GCAGTCAAGT CCAATGGCAG CGTCTTCGTC 
CGGGACTCCT CTCAGGTGCA CCCGCTAGCT GTGCTGCTGC TGACCGACGG GGACGTGCAC 
ATCCGTGATG ACGGGCGCCG GGCCACCATC TCACTGAGCG ACAGTGACCT GCTGCGGCTG 
GAGGGTGACT CGCGTACCGT GCGGCTGCTG AAGGAGCTGC GGCGGGCCCT GGGCCGCATG 
GTGGAGCGGA GCCTGCGCAG CGAGCTGGCT GCACTTCCCC CCAGCGTACA GGAGGAGCAC 
GGGCAGCTGC TTGCGCTACT GGCAGAGCTG CTGCGAGGAC CCTGTGGCAG CTTTGATGTG 
CGCAAGACAG CTGACGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_017005914.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861403.1 Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X2, mRNA.

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CloneID	OHu93898
Clone ID Related Accession (Same CDS sequence)	XM_017005914.1
Accession Version	XM_017005914.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX30 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACTGA AGTCTTCTTT GTGGGTTGCT GGACCAGGAA TATTAACCAT TGCCTCTTGG 
CCCTCCTTGT TCTCAGGATT CCAGCTTTCC CTCCTGGCCA GAAATGTTCA GCCTGGACTC 
ATTCAGAAAA GATCGGGCCC AGCACAGGCA GCGTCAGTGC AAACTTCCCC CACCCCGCCT 
TCCACCCATG TGTGTCAACC CTACCCCAGG AGGGACCATC TCTCGAGGCT GAACGTTAAC 
ATTTCCAACA TGGCAGCTTC TAGGGACCTA TTAAAAGAGT TCCCACAGCC CAAAAATCTT 
CTCAACAGTG TGATTGGAAG AGCCCTCGGC ATCTCACATG CAAAAGACAA ACTAGTCTAC 
GTGCACACAA ATGGACCGAA GAAAAAGAAA GTCACACTGC ACATAAAATG GCCCAAGAGC 
GTGGAGGTAG AAGGCTATGG CAGCAAGAAG ATCGATGCTG AGCGGCAGGC TGCAGCTGCA 
GCCTGCCAGC TGTTCAAGGG TTGGGGTCTG CTAGGTCCCC GGAATGAGTT GTTTGACGCA 
GCCAAATACC GAGTGCTAGC TGATCGCTTT GGCTCCCCTG CCGACAGCTG GTGGCGTCCG 
GAACCCACCA TGCCCCCTAC TTCCTGGCGG CAGCTGAATC CAGAGAGTAT TCGACCAGGG 
GGACCTGGGG GCCTATCCCG CTCTTTAGGC CGGGAAGAAG AGGAGGACGA GGAGGAAGAG 
CTAGAAGAAG GGACCATAGA TGTTACCGAC TTCTTGTCCA TGACCCAGCA GGATTCCCAC 
GCTCCACTCA GGGACTCAAG GGGGAGTTCC TTTGAGATGA CAGATGACGA CAGTGCCATT 
AGGGCTCTGA CCCAGTTTCC ACTTCCCAAG AACCTTCTGG CCAAGGTGAT TCAGATTGCA 
ACGTCATCCT CCACAGCTAA GAACCTCATG CAGTTCCATA CTGTGGGCAC CAAGACCAAG 
CTGTCTACAC TCACCCTGCT CTGGCCCTGC CCCATGACCT TTGTTGCCAA AGGGCGCCGC 
AAAGCAGAGG CTGAGAATAA GGCGGCAGCC TTGGCCTGCA AGAAACTGAA GAGCCTGGGC 
CTGGTGGACA GGAACAACGA ACCGCTTACA CACGCCATGT ATAACCTGGC CTCTTTGCGT 
GAGCTGGGTG AGACCCAGCG CCGACCATGC ACCATCCAGG TGCCCGAGCC CATCCTCCGC 
AAGATAGAGA CCTTCCTGAA CCATTACCCT GTGGAGAGTT CATGGATCGC CCCAGAACTC 
CGGCTGCAGA GTGATGACAT CTTGCCCTTG GGCAAGGACT CAGGGCCTCT GAGTGACCCT 
ATCACAGGCA AGCCCTATGT GCCCCTGTTG GAAGCAGAGG AGGTACGTCT CAGCCAGAGT 
CTGCTAGAAC TGTGGCGGCG GCGAGGGCCG GTCTGGCAGG AGGCCCCCCA GCTACCTGTG 
GACCCACATC GGGACACCAT CCTCAACGCC ATTGAGCAGC ACCCGGTGGT GGTCATCTCT 
GGGGACACGG GCTGTGGGAA GACCACGCGC ATCCCCCAGC TGTTGCTGGA GCGCTATGTG 
ACCGAGGGCC GAGGTGCCCG CTGCAATGTT ATCATCACCC AACCTCGCCG CATCTCTGCT 
GTGTCTGTGG CACAGCGGGT CAGCCACGAA CTGGGCCCCT CCCTGCGCCG GAATGTGGGC 
TTCCAGGTGC GGTTGGAAAG TAAGCCCCCA TCCCGAGGCG GGGCCCTGCT CTTCTGCACT 
GTGGGTATCC TGCTGCGTAA GCTGCAGAGC AACCCCAGCC TGGAGGGCGT GAGCCACGTC 
ATCGTGGATG AGGTGCATGA GCGGGACGTG AACACAGACT TTCTGCTGAT CCTGCTCAAG 
GGCCTGCAGC GGCTCAACCC GGCCCTGCGG CTGGTGCTCA TGAGTGCCAC AGGGGACAAT 
GAGCGCTTCT CCCGATACTT TGGTGGCTGC CCCGTCATCA AGGTGCCTGG CTTCATGTAC 
CCAGTCAAGG AGCACTACCT AGAGGACATC CTGGCCAAGT TGGGCAAGCA CCAGTACCTG 
CACCGGCACC GGCACCATGA GTCTGAGGAT GAATGCGCAC TCGATTTGGA CCTTGTGACT 
GATCTGGTTC TGCACATCGA TGCTCGCGGG GAACCAGGTG GGATCCTGTG CTTCCTGCCT 
GGGTGGCAGG AGATCAAAGG AGTGCAGCAG CGCCTCCAGG AGGCCCTGGG CATGCACGAG 
AGCAAGTACC TCATCCTGCC AGTGCACTCC AACATCCCCA TGATGGATCA GAAGGCCATA 
TTCCAGCAGC CTCCAGTTGG GGTGCGCAAG ATTGTCTTGG CCACCAACAT TGCTGAGACT 
TCCATCACAA TCAATGACAT CGTGCATGTG GTGGACAGTG GGCTGCACAA GGAAGAACGC 
TATGACCTGA AGACCAAGGT GTCCTGCCTG GAGACAGTGT GGGTATCAAG AGCCAATGTG 
ATCCAGCGCC GGGGCCGGGC GGGCCGCTGC CAGTCCGGCT TTGCCTACCA CTTGTTCCCT 
CGAAGCCGGC TGGAGAAAAT GGTCCCTTTC CAAGTGCCAG AGATCCTGCG CACACCTCTT 
GAGAACCTGG TGCTGCAAGC GAAAATCCAC ATGCCTGAGA AGACGGCGGT GGAGTTCCTG 
TCCAAGGCTG TGGACAGTCC AAACATCAAG GCAGTGGACG AGGCTGTGAT CTTGCTCCAG 
GAGATCGGGG TGCTGGACCA GCGGGAGTAC CTGACTACCC TGGGGCAGCG CCTGGCTCAC 
ATCTCCACCG ACCCCCGGTT GGCCAAGGCC ATTGTGTTGG CTGCCATCTT CCGTTGCCTG 
CACCCACTAC TGGTGGTCGT TTCCTGCCTC ACCCGGGACC CCTTCAGCAG CAGCCTACAG 
AACCGGGCAG AGGTGGACAA GGTGAAAGCA CTGTTGAGCC ATGACAGCGG CAGTGACCAC 
CTGGCCTTTG TGCGGGCTGT CGCCGGCTGG GAGGAGGTGC TGCGTTGGCA GGACCGCAGC 
TCCCGGGAGA ATTACCTGGA GGAAAACCTG CTGTACGCAC CCAGCCTGCG CTTCATCCAC 
GGACTCATCA AGCAGTTCTC AGAGAACATT TATGAGGCCT TCCTGGTGGG GAAGCCCTCG 
GACTGCACCC TGGCCTCCGC CCAGTGCAAC GAGTACAGTG AGGAGGAGGA GCTGGTGAAG 
GGCGTGCTGA TGGCCGGCCT CTACCCCAAC CTCATCCAGG TGAGGCAGGG CAAGGTCACC 
CGGCAGGGGA AGTTCAAGCC CAACAGCGTC ACATATAGGA CCAAATCAGG CAACATCCTG 
CTGCACAAGT CGACCATTAA CAGGGAGGCC ACACGGTTAC GGAGCCGATG GCTGACGTAT 
TTCATGGCAG TCAAGTCCAA TGGCAGCGTC TTCGTCCGGG ACTCCTCTCA GGTGCACCCG 
CTAGCTGTGC TGCTGCTGAC CGACGGGGAC GTGCACATCC GTGATGACGG GCGCCGGGCC 
ACCATCTCAC TGAGCGACAG TGACCTGCTG CGGCTGGAGG GTGACTCGCG TACCGTGCGG 
CTGCTGAAGG AGCTGCGGCG GGCCCTGGGC CGCATGGTGG AGCGGAGCCT GCGCAGCGAG 
CTGGCTGCAC TTCCCCCCAG CGTACAGGAG GAGCACGGGC AGCTGCTTGC GCTACTGGCA 
GAGCTGCTGC GAGGACCCTG TGGCAGCTTT GATGTGCGCA AGACAGCTGA CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861403.1
CDS	200..3916
Translation	MLLKSSLWVAGPGILTIASWPSLFSGFQLSLLARNVQPGLIQKRSGPAQAASVQTSPTPPSTHVCQPYPRRDHLSRLNVNISNMAASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_017005914.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005914.1

ORF Insert Sequence:

ATGTTACTGA AGTCTTCTTT GTGGGTTGCT GGACCAGGAA TATTAACCAT TGCCTCTTGG 
CCCTCCTTGT TCTCAGGATT CCAGCTTTCC CTCCTGGCCA GAAATGTTCA GCCTGGACTC 
ATTCAGAAAA GATCGGGCCC AGCACAGGCA GCGTCAGTGC AAACTTCCCC CACCCCGCCT 
TCCACCCATG TGTGTCAACC CTACCCCAGG AGGGACCATC TCTCGAGGCT GAACGTTAAC 
ATTTCCAACA TGGCAGCTTC TAGGGACCTA TTAAAAGAGT TCCCACAGCC CAAAAATCTT 
CTCAACAGTG TGATTGGAAG AGCCCTCGGC ATCTCACATG CAAAAGACAA ACTAGTCTAC 
GTGCACACAA ATGGACCGAA GAAAAAGAAA GTCACACTGC ACATAAAATG GCCCAAGAGC 
GTGGAGGTAG AAGGCTATGG CAGCAAGAAG ATCGATGCTG AGCGGCAGGC TGCAGCTGCA 
GCCTGCCAGC TGTTCAAGGG TTGGGGTCTG CTAGGTCCCC GGAATGAGTT GTTTGACGCA 
GCCAAATACC GAGTGCTAGC TGATCGCTTT GGCTCCCCTG CCGACAGCTG GTGGCGTCCG 
GAACCCACCA TGCCCCCTAC TTCCTGGCGG CAGCTGAATC CAGAGAGTAT TCGACCAGGG 
GGACCTGGGG GCCTATCCCG CTCTTTAGGC CGGGAAGAAG AGGAGGACGA GGAGGAAGAG 
CTAGAAGAAG GGACCATAGA TGTTACCGAC TTCTTGTCCA TGACCCAGCA GGATTCCCAC 
GCTCCACTCA GGGACTCAAG GGGGAGTTCC TTTGAGATGA CAGATGACGA CAGTGCCATT 
AGGGCTCTGA CCCAGTTTCC ACTTCCCAAG AACCTTCTGG CCAAGGTGAT TCAGATTGCA 
ACGTCATCCT CCACAGCTAA GAACCTCATG CAGTTCCATA CTGTGGGCAC CAAGACCAAG 
CTGTCTACAC TCACCCTGCT CTGGCCCTGC CCCATGACCT TTGTTGCCAA AGGGCGCCGC 
AAAGCAGAGG CTGAGAATAA GGCGGCAGCC TTGGCCTGCA AGAAACTGAA GAGCCTGGGC 
CTGGTGGACA GGAACAACGA ACCGCTTACA CACGCCATGT ATAACCTGGC CTCTTTGCGT 
GAGCTGGGTG AGACCCAGCG CCGACCATGC ACCATCCAGG TGCCCGAGCC CATCCTCCGC 
AAGATAGAGA CCTTCCTGAA CCATTACCCT GTGGAGAGTT CATGGATCGC CCCAGAACTC 
CGGCTGCAGA GTGATGACAT CTTGCCCTTG GGCAAGGACT CAGGGCCTCT GAGTGACCCT 
ATCACAGGCA AGCCCTATGT GCCCCTGTTG GAAGCAGAGG AGGTACGTCT CAGCCAGAGT 
CTGCTAGAAC TGTGGCGGCG GCGAGGGCCG GTCTGGCAGG AGGCCCCCCA GCTACCTGTG 
GACCCACATC GGGACACCAT CCTCAACGCC ATTGAGCAGC ACCCGGTGGT GGTCATCTCT 
GGGGACACGG GCTGTGGGAA GACCACGCGC ATCCCCCAGC TGTTGCTGGA GCGCTATGTG 
ACCGAGGGCC GAGGTGCCCG CTGCAATGTT ATCATCACCC AACCTCGCCG CATCTCTGCT 
GTGTCTGTGG CACAGCGGGT CAGCCACGAA CTGGGCCCCT CCCTGCGCCG GAATGTGGGC 
TTCCAGGTGC GGTTGGAAAG TAAGCCCCCA TCCCGAGGCG GGGCCCTGCT CTTCTGCACT 
GTGGGTATCC TGCTGCGTAA GCTGCAGAGC AACCCCAGCC TGGAGGGCGT GAGCCACGTC 
ATCGTGGATG AGGTGCATGA GCGGGACGTG AACACAGACT TTCTGCTGAT CCTGCTCAAG 
GGCCTGCAGC GGCTCAACCC GGCCCTGCGG CTGGTGCTCA TGAGTGCCAC AGGGGACAAT 
GAGCGCTTCT CCCGATACTT TGGTGGCTGC CCCGTCATCA AGGTGCCTGG CTTCATGTAC 
CCAGTCAAGG AGCACTACCT AGAGGACATC CTGGCCAAGT TGGGCAAGCA CCAGTACCTG 
CACCGGCACC GGCACCATGA GTCTGAGGAT GAATGCGCAC TCGATTTGGA CCTTGTGACT 
GATCTGGTTC TGCACATCGA TGCTCGCGGG GAACCAGGTG GGATCCTGTG CTTCCTGCCT 
GGGTGGCAGG AGATCAAAGG AGTGCAGCAG CGCCTCCAGG AGGCCCTGGG CATGCACGAG 
AGCAAGTACC TCATCCTGCC AGTGCACTCC AACATCCCCA TGATGGATCA GAAGGCCATA 
TTCCAGCAGC CTCCAGTTGG GGTGCGCAAG ATTGTCTTGG CCACCAACAT TGCTGAGACT 
TCCATCACAA TCAATGACAT CGTGCATGTG GTGGACAGTG GGCTGCACAA GGAAGAACGC 
TATGACCTGA AGACCAAGGT GTCCTGCCTG GAGACAGTGT GGGTATCAAG AGCCAATGTG 
ATCCAGCGCC GGGGCCGGGC GGGCCGCTGC CAGTCCGGCT TTGCCTACCA CTTGTTCCCT 
CGAAGCCGGC TGGAGAAAAT GGTCCCTTTC CAAGTGCCAG AGATCCTGCG CACACCTCTT 
GAGAACCTGG TGCTGCAAGC GAAAATCCAC ATGCCTGAGA AGACGGCGGT GGAGTTCCTG 
TCCAAGGCTG TGGACAGTCC AAACATCAAG GCAGTGGACG AGGCTGTGAT CTTGCTCCAG 
GAGATCGGGG TGCTGGACCA GCGGGAGTAC CTGACTACCC TGGGGCAGCG CCTGGCTCAC 
ATCTCCACCG ACCCCCGGTT GGCCAAGGCC ATTGTGTTGG CTGCCATCTT CCGTTGCCTG 
CACCCACTAC TGGTGGTCGT TTCCTGCCTC ACCCGGGACC CCTTCAGCAG CAGCCTACAG 
AACCGGGCAG AGGTGGACAA GGTGAAAGCA CTGTTGAGCC ATGACAGCGG CAGTGACCAC 
CTGGCCTTTG TGCGGGCTGT CGCCGGCTGG GAGGAGGTGC TGCGTTGGCA GGACCGCAGC 
TCCCGGGAGA ATTACCTGGA GGAAAACCTG CTGTACGCAC CCAGCCTGCG CTTCATCCAC 
GGACTCATCA AGCAGTTCTC AGAGAACATT TATGAGGCCT TCCTGGTGGG GAAGCCCTCG 
GACTGCACCC TGGCCTCCGC CCAGTGCAAC GAGTACAGTG AGGAGGAGGA GCTGGTGAAG 
GGCGTGCTGA TGGCCGGCCT CTACCCCAAC CTCATCCAGG TGAGGCAGGG CAAGGTCACC 
CGGCAGGGGA AGTTCAAGCC CAACAGCGTC ACATATAGGA CCAAATCAGG CAACATCCTG 
CTGCACAAGT CGACCATTAA CAGGGAGGCC ACACGGTTAC GGAGCCGATG GCTGACGTAT 
TTCATGGCAG TCAAGTCCAA TGGCAGCGTC TTCGTCCGGG ACTCCTCTCA GGTGCACCCG 
CTAGCTGTGC TGCTGCTGAC CGACGGGGAC GTGCACATCC GTGATGACGG GCGCCGGGCC 
ACCATCTCAC TGAGCGACAG TGACCTGCTG CGGCTGGAGG GTGACTCGCG TACCGTGCGG 
CTGCTGAAGG AGCTGCGGCG GGCCCTGGGC CGCATGGTGG AGCGGAGCCT GCGCAGCGAG 
CTGGCTGCAC TTCCCCCCAG CGTACAGGAG GAGCACGGGC AGCTGCTTGC GCTACTGGCA 
GAGCTGCTGC GAGGACCCTG TGGCAGCTTT GATGTGCGCA AGACAGCTGA CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_017005916.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861405.1 Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X10, mRNA.

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CloneID	OHu93899
Clone ID Related Accession (Same CDS sequence)	XM_017005916.2 , XM_017005916.1
Accession Version	XM_017005916.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX30 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005916.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGGGTTG GGGTCTGCTA GGTCCCCGGA ATGAGTTGTT TGACGCAGCC 
AAATACCGAG TGCTAGCTGA TCGCTTTGGC TCCCCTGCCG ACAGCTGGTG GCGTCCGGAA 
CCCACCATGC CCCCTACTTC CTGGCGGCAG CTGAATCCAG AGAGTATTCG ACCAGGGGGA 
CCTGGGGGCC TATCCCGCTC TTTAGGCCGG GAAGAAGAGG AGGACGAGGA GGAAGAGCTA 
GAAGAAGGGA CCATAGATGT TACCGACTTC TTGTCCATGA CCCAGCAGGA TTCCCACGCT 
CCACTCAGGG ACTCAAGGGG GAGTTCCTTT GAGATGACAG ATGACGACAG TGCCATTAGG 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTTCTGGCCA AGGTGATTCA GATTGCAACG 
TCATCCTCCA CAGCTAAGAA CCTCATGCAG TTCCATACTG TGGGCACCAA GACCAAGCTG 
TCTACACTCA CCCTGCTCTG GCCCTGCCCC ATGACCTTTG TTGCCAAAGG GCGCCGCAAA 
GCAGAGGCTG AGAATAAGGC GGCAGCCTTG GCCTGCAAGA AACTGAAGAG CCTGGGCCTG 
GTGGACAGGA ACAACGAACC GCTTACACAC GCCATGTATA ACCTGGCCTC TTTGCGTGAG 
CTGGGTGAGA CCCAGCGCCG ACCATGCACC ATCCAGGTGC CCGAGCCCAT CCTCCGCAAG 
ATAGAGACCT TCCTGAACCA TTACCCTGTG GAGAGTTCAT GGATCGCCCC AGAACTCCGG 
CTGCAGAGTG ATGACATCTT GCCCTTGGGC AAGGACTCAG GGCCTCTGAG TGACCCTATC 
ACAGGCAAGC CCTATGTGCC CCTGTTGGAA GCAGAGGAGG TACGTCTCAG CCAGAGTCTG 
CTAGAACTGT GGCGGCGGCG AGGGCCGGTC TGGCAGGAGG CCCCCCAGCT ACCTGTGGAC 
CCACATCGGG ACACCATCCT CAACGCCATT GAGCAGCACC CGGTGGTGGT CATCTCTGGG 
GACACGGGCT GTGGGAAGAC CACGCGCATC CCCCAGCTGT TGCTGGAGCG CTATGTGACC 
GAGGGCCGAG GTGCCCGCTG CAATGTTATC ATCACCCAAC CTCGCCGCAT CTCTGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCACGAACTG GGCCCCTCCC TGCGCCGGAA TGTGGGCTTC 
CAGGTGCGGT TGGAAAGTAA GCCCCCATCC CGAGGCGGGG CCCTGCTCTT CTGCACTGTG 
GGTATCCTGC TGCGTAAGCT GCAGAGCAAC CCCAGCCTGG AGGGCGTGAG CCACGTCATC 
GTGGATGAGG TGCATGAGCG GGACGTGAAC ACAGACTTTC TGCTGATCCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCGGC CCTGCGGCTG GTGCTCATGA GTGCCACAGG GGACAATGAG 
CGCTTCTCCC GATACTTTGG TGGCTGCCCC GTCATCAAGG TGCCTGGCTT CATGTACCCA 
GTCAAGGAGC ACTACCTAGA GGACATCCTG GCCAAGTTGG GCAAGCACCA GTACCTGCAC 
CGGCACCGGC ACCATGAGTC TGAGGATGAA TGCGCACTCG ATTTGGACCT TGTGACTGAT 
CTGGTTCTGC ACATCGATGC TCGCGGGGAA CCAGGTGGGA TCCTGTGCTT CCTGCCTGGG 
TGGCAGGAGA TCAAAGGAGT GCAGCAGCGC CTCCAGGAGG CCCTGGGCAT GCACGAGAGC 
AAGTACCTCA TCCTGCCAGT GCACTCCAAC ATCCCCATGA TGGATCAGAA GGCCATATTC 
CAGCAGCCTC CAGTTGGGGT GCGCAAGATT GTCTTGGCCA CCAACATTGC TGAGACTTCC 
ATCACAATCA ATGACATCGT GCATGTGGTG GACAGTGGGC TGCACAAGGA AGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACAGTGTGGG TATCAAGAGC CAATGTGATC 
CAGCGCCGGG GCCGGGCGGG CCGCTGCCAG TCCGGCTTTG CCTACCACTT GTTCCCTCGA 
AGCCGGCTGG AGAAAATGGT CCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCGAA AATCCACATG CCTGAGAAGA CGGCGGTGGA GTTCCTGTCC 
AAGGCTGTGG ACAGTCCAAA CATCAAGGCA GTGGACGAGG CTGTGATCTT GCTCCAGGAG 
ATCGGGGTGC TGGACCAGCG GGAGTACCTG ACTACCCTGG GGCAGCGCCT GGCTCACATC 
TCCACCGACC CCCGGTTGGC CAAGGCCATT GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTACTGG TGGTCGTTTC CTGCCTCACC CGGGACCCCT TCAGCAGCAG CCTACAGAAC 
CGGGCAGAGG TGGACAAGGT GAAAGCACTG TTGAGCCATG ACAGCGGCAG TGACCACCTG 
GCCTTTGTGC GGGCTGTCGC CGGCTGGGAG GAGGTGCTGC GTTGGCAGGA CCGCAGCTCC 
CGGGAGAATT ACCTGGAGGA AAACCTGCTG TACGCACCCA GCCTGCGCTT CATCCACGGA 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCCTTCC TGGTGGGGAA GCCCTCGGAC 
TGCACCCTGG CCTCCGCCCA GTGCAACGAG TACAGTGAGG AGGAGGAGCT GGTGAAGGGC 
GTGCTGATGG CCGGCCTCTA CCCCAACCTC ATCCAGGTGA GGCAGGGCAA GGTCACCCGG 
CAGGGGAAGT TCAAGCCCAA CAGCGTCACA TATAGGACCA AATCAGGCAA CATCCTGCTG 
CACAAGTCGA CCATTAACAG GGAGGCCACA CGGTTACGGA GCCGATGGCT GACGTATTTC 
ATGGCAGTCA AGTCCAATGG CAGCGTCTTC GTCCGGGACT CCTCTCAGGT GCACCCGCTA 
GCTGTGCTGC TGCTGACCGA CGGGGACGTG CACATCCGTG ATGACGGGCG CCGGGCCACC 
ATCTCACTGA GCGACAGTGA CCTGCTGCGG CTGGAGGGTG ACTCGCGTAC CGTGCGGCTG 
CTGAAGGAGC TGCGGCGGGC CCTGGGCCGC ATGGTGGAGC GGAGCCTGCG CAGCGAGCTG 
GCTGCACTTC CCCCCAGCGT ACAGGAGGAG CACGGGCAGC TGCTTGCGCT ACTGGCAGAG 
CTGCTGCGAG GACCCTGTGG CAGCTTTGAT GTGCGCAAGA CAGCTGACGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861405.1
CDS	220..3693
Translation	MFSLDSFRKDRAQHRQRQCKLPPPRLPPMCVNPTPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_017005916.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005916.2

ORF Insert Sequence:

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGGGTTG GGGTCTGCTA GGTCCCCGGA ATGAGTTGTT TGACGCAGCC 
AAATACCGAG TGCTAGCTGA TCGCTTTGGC TCCCCTGCCG ACAGCTGGTG GCGTCCGGAA 
CCCACCATGC CCCCTACTTC CTGGCGGCAG CTGAATCCAG AGAGTATTCG ACCAGGGGGA 
CCTGGGGGCC TATCCCGCTC TTTAGGCCGG GAAGAAGAGG AGGACGAGGA GGAAGAGCTA 
GAAGAAGGGA CCATAGATGT TACCGACTTC TTGTCCATGA CCCAGCAGGA TTCCCACGCT 
CCACTCAGGG ACTCAAGGGG GAGTTCCTTT GAGATGACAG ATGACGACAG TGCCATTAGG 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTTCTGGCCA AGGTGATTCA GATTGCAACG 
TCATCCTCCA CAGCTAAGAA CCTCATGCAG TTCCATACTG TGGGCACCAA GACCAAGCTG 
TCTACACTCA CCCTGCTCTG GCCCTGCCCC ATGACCTTTG TTGCCAAAGG GCGCCGCAAA 
GCAGAGGCTG AGAATAAGGC GGCAGCCTTG GCCTGCAAGA AACTGAAGAG CCTGGGCCTG 
GTGGACAGGA ACAACGAACC GCTTACACAC GCCATGTATA ACCTGGCCTC TTTGCGTGAG 
CTGGGTGAGA CCCAGCGCCG ACCATGCACC ATCCAGGTGC CCGAGCCCAT CCTCCGCAAG 
ATAGAGACCT TCCTGAACCA TTACCCTGTG GAGAGTTCAT GGATCGCCCC AGAACTCCGG 
CTGCAGAGTG ATGACATCTT GCCCTTGGGC AAGGACTCAG GGCCTCTGAG TGACCCTATC 
ACAGGCAAGC CCTATGTGCC CCTGTTGGAA GCAGAGGAGG TACGTCTCAG CCAGAGTCTG 
CTAGAACTGT GGCGGCGGCG AGGGCCGGTC TGGCAGGAGG CCCCCCAGCT ACCTGTGGAC 
CCACATCGGG ACACCATCCT CAACGCCATT GAGCAGCACC CGGTGGTGGT CATCTCTGGG 
GACACGGGCT GTGGGAAGAC CACGCGCATC CCCCAGCTGT TGCTGGAGCG CTATGTGACC 
GAGGGCCGAG GTGCCCGCTG CAATGTTATC ATCACCCAAC CTCGCCGCAT CTCTGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCACGAACTG GGCCCCTCCC TGCGCCGGAA TGTGGGCTTC 
CAGGTGCGGT TGGAAAGTAA GCCCCCATCC CGAGGCGGGG CCCTGCTCTT CTGCACTGTG 
GGTATCCTGC TGCGTAAGCT GCAGAGCAAC CCCAGCCTGG AGGGCGTGAG CCACGTCATC 
GTGGATGAGG TGCATGAGCG GGACGTGAAC ACAGACTTTC TGCTGATCCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCGGC CCTGCGGCTG GTGCTCATGA GTGCCACAGG GGACAATGAG 
CGCTTCTCCC GATACTTTGG TGGCTGCCCC GTCATCAAGG TGCCTGGCTT CATGTACCCA 
GTCAAGGAGC ACTACCTAGA GGACATCCTG GCCAAGTTGG GCAAGCACCA GTACCTGCAC 
CGGCACCGGC ACCATGAGTC TGAGGATGAA TGCGCACTCG ATTTGGACCT TGTGACTGAT 
CTGGTTCTGC ACATCGATGC TCGCGGGGAA CCAGGTGGGA TCCTGTGCTT CCTGCCTGGG 
TGGCAGGAGA TCAAAGGAGT GCAGCAGCGC CTCCAGGAGG CCCTGGGCAT GCACGAGAGC 
AAGTACCTCA TCCTGCCAGT GCACTCCAAC ATCCCCATGA TGGATCAGAA GGCCATATTC 
CAGCAGCCTC CAGTTGGGGT GCGCAAGATT GTCTTGGCCA CCAACATTGC TGAGACTTCC 
ATCACAATCA ATGACATCGT GCATGTGGTG GACAGTGGGC TGCACAAGGA AGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACAGTGTGGG TATCAAGAGC CAATGTGATC 
CAGCGCCGGG GCCGGGCGGG CCGCTGCCAG TCCGGCTTTG CCTACCACTT GTTCCCTCGA 
AGCCGGCTGG AGAAAATGGT CCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCGAA AATCCACATG CCTGAGAAGA CGGCGGTGGA GTTCCTGTCC 
AAGGCTGTGG ACAGTCCAAA CATCAAGGCA GTGGACGAGG CTGTGATCTT GCTCCAGGAG 
ATCGGGGTGC TGGACCAGCG GGAGTACCTG ACTACCCTGG GGCAGCGCCT GGCTCACATC 
TCCACCGACC CCCGGTTGGC CAAGGCCATT GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTACTGG TGGTCGTTTC CTGCCTCACC CGGGACCCCT TCAGCAGCAG CCTACAGAAC 
CGGGCAGAGG TGGACAAGGT GAAAGCACTG TTGAGCCATG ACAGCGGCAG TGACCACCTG 
GCCTTTGTGC GGGCTGTCGC CGGCTGGGAG GAGGTGCTGC GTTGGCAGGA CCGCAGCTCC 
CGGGAGAATT ACCTGGAGGA AAACCTGCTG TACGCACCCA GCCTGCGCTT CATCCACGGA 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCCTTCC TGGTGGGGAA GCCCTCGGAC 
TGCACCCTGG CCTCCGCCCA GTGCAACGAG TACAGTGAGG AGGAGGAGCT GGTGAAGGGC 
GTGCTGATGG CCGGCCTCTA CCCCAACCTC ATCCAGGTGA GGCAGGGCAA GGTCACCCGG 
CAGGGGAAGT TCAAGCCCAA CAGCGTCACA TATAGGACCA AATCAGGCAA CATCCTGCTG 
CACAAGTCGA CCATTAACAG GGAGGCCACA CGGTTACGGA GCCGATGGCT GACGTATTTC 
ATGGCAGTCA AGTCCAATGG CAGCGTCTTC GTCCGGGACT CCTCTCAGGT GCACCCGCTA 
GCTGTGCTGC TGCTGACCGA CGGGGACGTG CACATCCGTG ATGACGGGCG CCGGGCCACC 
ATCTCACTGA GCGACAGTGA CCTGCTGCGG CTGGAGGGTG ACTCGCGTAC CGTGCGGCTG 
CTGAAGGAGC TGCGGCGGGC CCTGGGCCGC ATGGTGGAGC GGAGCCTGCG CAGCGAGCTG 
GCTGCACTTC CCCCCAGCGT ACAGGAGGAG CACGGGCAGC TGCTTGCGCT ACTGGCAGAG 
CTGCTGCGAG GACCCTGTGG CAGCTTTGAT GTGCGCAAGA CAGCTGACGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_017005916.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861405.1 Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu93899
Clone ID Related Accession (Same CDS sequence)	XM_017005916.2 , XM_017005916.1
Accession Version	XM_017005916.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative ATP-dependent RNA helicase DHX30 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGGGTTG GGGTCTGCTA GGTCCCCGGA ATGAGTTGTT TGACGCAGCC 
AAATACCGAG TGCTAGCTGA TCGCTTTGGC TCCCCTGCCG ACAGCTGGTG GCGTCCGGAA 
CCCACCATGC CCCCTACTTC CTGGCGGCAG CTGAATCCAG AGAGTATTCG ACCAGGGGGA 
CCTGGGGGCC TATCCCGCTC TTTAGGCCGG GAAGAAGAGG AGGACGAGGA GGAAGAGCTA 
GAAGAAGGGA CCATAGATGT TACCGACTTC TTGTCCATGA CCCAGCAGGA TTCCCACGCT 
CCACTCAGGG ACTCAAGGGG GAGTTCCTTT GAGATGACAG ATGACGACAG TGCCATTAGG 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTTCTGGCCA AGGTGATTCA GATTGCAACG 
TCATCCTCCA CAGCTAAGAA CCTCATGCAG TTCCATACTG TGGGCACCAA GACCAAGCTG 
TCTACACTCA CCCTGCTCTG GCCCTGCCCC ATGACCTTTG TTGCCAAAGG GCGCCGCAAA 
GCAGAGGCTG AGAATAAGGC GGCAGCCTTG GCCTGCAAGA AACTGAAGAG CCTGGGCCTG 
GTGGACAGGA ACAACGAACC GCTTACACAC GCCATGTATA ACCTGGCCTC TTTGCGTGAG 
CTGGGTGAGA CCCAGCGCCG ACCATGCACC ATCCAGGTGC CCGAGCCCAT CCTCCGCAAG 
ATAGAGACCT TCCTGAACCA TTACCCTGTG GAGAGTTCAT GGATCGCCCC AGAACTCCGG 
CTGCAGAGTG ATGACATCTT GCCCTTGGGC AAGGACTCAG GGCCTCTGAG TGACCCTATC 
ACAGGCAAGC CCTATGTGCC CCTGTTGGAA GCAGAGGAGG TACGTCTCAG CCAGAGTCTG 
CTAGAACTGT GGCGGCGGCG AGGGCCGGTC TGGCAGGAGG CCCCCCAGCT ACCTGTGGAC 
CCACATCGGG ACACCATCCT CAACGCCATT GAGCAGCACC CGGTGGTGGT CATCTCTGGG 
GACACGGGCT GTGGGAAGAC CACGCGCATC CCCCAGCTGT TGCTGGAGCG CTATGTGACC 
GAGGGCCGAG GTGCCCGCTG CAATGTTATC ATCACCCAAC CTCGCCGCAT CTCTGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCACGAACTG GGCCCCTCCC TGCGCCGGAA TGTGGGCTTC 
CAGGTGCGGT TGGAAAGTAA GCCCCCATCC CGAGGCGGGG CCCTGCTCTT CTGCACTGTG 
GGTATCCTGC TGCGTAAGCT GCAGAGCAAC CCCAGCCTGG AGGGCGTGAG CCACGTCATC 
GTGGATGAGG TGCATGAGCG GGACGTGAAC ACAGACTTTC TGCTGATCCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCGGC CCTGCGGCTG GTGCTCATGA GTGCCACAGG GGACAATGAG 
CGCTTCTCCC GATACTTTGG TGGCTGCCCC GTCATCAAGG TGCCTGGCTT CATGTACCCA 
GTCAAGGAGC ACTACCTAGA GGACATCCTG GCCAAGTTGG GCAAGCACCA GTACCTGCAC 
CGGCACCGGC ACCATGAGTC TGAGGATGAA TGCGCACTCG ATTTGGACCT TGTGACTGAT 
CTGGTTCTGC ACATCGATGC TCGCGGGGAA CCAGGTGGGA TCCTGTGCTT CCTGCCTGGG 
TGGCAGGAGA TCAAAGGAGT GCAGCAGCGC CTCCAGGAGG CCCTGGGCAT GCACGAGAGC 
AAGTACCTCA TCCTGCCAGT GCACTCCAAC ATCCCCATGA TGGATCAGAA GGCCATATTC 
CAGCAGCCTC CAGTTGGGGT GCGCAAGATT GTCTTGGCCA CCAACATTGC TGAGACTTCC 
ATCACAATCA ATGACATCGT GCATGTGGTG GACAGTGGGC TGCACAAGGA AGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACAGTGTGGG TATCAAGAGC CAATGTGATC 
CAGCGCCGGG GCCGGGCGGG CCGCTGCCAG TCCGGCTTTG CCTACCACTT GTTCCCTCGA 
AGCCGGCTGG AGAAAATGGT CCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCGAA AATCCACATG CCTGAGAAGA CGGCGGTGGA GTTCCTGTCC 
AAGGCTGTGG ACAGTCCAAA CATCAAGGCA GTGGACGAGG CTGTGATCTT GCTCCAGGAG 
ATCGGGGTGC TGGACCAGCG GGAGTACCTG ACTACCCTGG GGCAGCGCCT GGCTCACATC 
TCCACCGACC CCCGGTTGGC CAAGGCCATT GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTACTGG TGGTCGTTTC CTGCCTCACC CGGGACCCCT TCAGCAGCAG CCTACAGAAC 
CGGGCAGAGG TGGACAAGGT GAAAGCACTG TTGAGCCATG ACAGCGGCAG TGACCACCTG 
GCCTTTGTGC GGGCTGTCGC CGGCTGGGAG GAGGTGCTGC GTTGGCAGGA CCGCAGCTCC 
CGGGAGAATT ACCTGGAGGA AAACCTGCTG TACGCACCCA GCCTGCGCTT CATCCACGGA 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCCTTCC TGGTGGGGAA GCCCTCGGAC 
TGCACCCTGG CCTCCGCCCA GTGCAACGAG TACAGTGAGG AGGAGGAGCT GGTGAAGGGC 
GTGCTGATGG CCGGCCTCTA CCCCAACCTC ATCCAGGTGA GGCAGGGCAA GGTCACCCGG 
CAGGGGAAGT TCAAGCCCAA CAGCGTCACA TATAGGACCA AATCAGGCAA CATCCTGCTG 
CACAAGTCGA CCATTAACAG GGAGGCCACA CGGTTACGGA GCCGATGGCT GACGTATTTC 
ATGGCAGTCA AGTCCAATGG CAGCGTCTTC GTCCGGGACT CCTCTCAGGT GCACCCGCTA 
GCTGTGCTGC TGCTGACCGA CGGGGACGTG CACATCCGTG ATGACGGGCG CCGGGCCACC 
ATCTCACTGA GCGACAGTGA CCTGCTGCGG CTGGAGGGTG ACTCGCGTAC CGTGCGGCTG 
CTGAAGGAGC TGCGGCGGGC CCTGGGCCGC ATGGTGGAGC GGAGCCTGCG CAGCGAGCTG 
GCTGCACTTC CCCCCAGCGT ACAGGAGGAG CACGGGCAGC TGCTTGCGCT ACTGGCAGAG 
CTGCTGCGAG GACCCTGTGG CAGCTTTGAT GTGCGCAAGA CAGCTGACGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861405.1
CDS	403..3876
Translation	MFSLDSFRKDRAQHRQRQCKLPPPRLPPMCVNPTPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_017005916.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005916.1

ORF Insert Sequence:

ATGTTCAGCC TGGACTCATT CAGAAAAGAT CGGGCCCAGC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGCCTTCC ACCCATGTGT GTCAACCCTA CCCCAGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTC CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CCCTCGGCAT CTCACATGCA AAAGACAAAC TAGTCTACGT GCACACAAAT 
GGACCGAAGA AAAAGGGTTG GGGTCTGCTA GGTCCCCGGA ATGAGTTGTT TGACGCAGCC 
AAATACCGAG TGCTAGCTGA TCGCTTTGGC TCCCCTGCCG ACAGCTGGTG GCGTCCGGAA 
CCCACCATGC CCCCTACTTC CTGGCGGCAG CTGAATCCAG AGAGTATTCG ACCAGGGGGA 
CCTGGGGGCC TATCCCGCTC TTTAGGCCGG GAAGAAGAGG AGGACGAGGA GGAAGAGCTA 
GAAGAAGGGA CCATAGATGT TACCGACTTC TTGTCCATGA CCCAGCAGGA TTCCCACGCT 
CCACTCAGGG ACTCAAGGGG GAGTTCCTTT GAGATGACAG ATGACGACAG TGCCATTAGG 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTTCTGGCCA AGGTGATTCA GATTGCAACG 
TCATCCTCCA CAGCTAAGAA CCTCATGCAG TTCCATACTG TGGGCACCAA GACCAAGCTG 
TCTACACTCA CCCTGCTCTG GCCCTGCCCC ATGACCTTTG TTGCCAAAGG GCGCCGCAAA 
GCAGAGGCTG AGAATAAGGC GGCAGCCTTG GCCTGCAAGA AACTGAAGAG CCTGGGCCTG 
GTGGACAGGA ACAACGAACC GCTTACACAC GCCATGTATA ACCTGGCCTC TTTGCGTGAG 
CTGGGTGAGA CCCAGCGCCG ACCATGCACC ATCCAGGTGC CCGAGCCCAT CCTCCGCAAG 
ATAGAGACCT TCCTGAACCA TTACCCTGTG GAGAGTTCAT GGATCGCCCC AGAACTCCGG 
CTGCAGAGTG ATGACATCTT GCCCTTGGGC AAGGACTCAG GGCCTCTGAG TGACCCTATC 
ACAGGCAAGC CCTATGTGCC CCTGTTGGAA GCAGAGGAGG TACGTCTCAG CCAGAGTCTG 
CTAGAACTGT GGCGGCGGCG AGGGCCGGTC TGGCAGGAGG CCCCCCAGCT ACCTGTGGAC 
CCACATCGGG ACACCATCCT CAACGCCATT GAGCAGCACC CGGTGGTGGT CATCTCTGGG 
GACACGGGCT GTGGGAAGAC CACGCGCATC CCCCAGCTGT TGCTGGAGCG CTATGTGACC 
GAGGGCCGAG GTGCCCGCTG CAATGTTATC ATCACCCAAC CTCGCCGCAT CTCTGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCACGAACTG GGCCCCTCCC TGCGCCGGAA TGTGGGCTTC 
CAGGTGCGGT TGGAAAGTAA GCCCCCATCC CGAGGCGGGG CCCTGCTCTT CTGCACTGTG 
GGTATCCTGC TGCGTAAGCT GCAGAGCAAC CCCAGCCTGG AGGGCGTGAG CCACGTCATC 
GTGGATGAGG TGCATGAGCG GGACGTGAAC ACAGACTTTC TGCTGATCCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCGGC CCTGCGGCTG GTGCTCATGA GTGCCACAGG GGACAATGAG 
CGCTTCTCCC GATACTTTGG TGGCTGCCCC GTCATCAAGG TGCCTGGCTT CATGTACCCA 
GTCAAGGAGC ACTACCTAGA GGACATCCTG GCCAAGTTGG GCAAGCACCA GTACCTGCAC 
CGGCACCGGC ACCATGAGTC TGAGGATGAA TGCGCACTCG ATTTGGACCT TGTGACTGAT 
CTGGTTCTGC ACATCGATGC TCGCGGGGAA CCAGGTGGGA TCCTGTGCTT CCTGCCTGGG 
TGGCAGGAGA TCAAAGGAGT GCAGCAGCGC CTCCAGGAGG CCCTGGGCAT GCACGAGAGC 
AAGTACCTCA TCCTGCCAGT GCACTCCAAC ATCCCCATGA TGGATCAGAA GGCCATATTC 
CAGCAGCCTC CAGTTGGGGT GCGCAAGATT GTCTTGGCCA CCAACATTGC TGAGACTTCC 
ATCACAATCA ATGACATCGT GCATGTGGTG GACAGTGGGC TGCACAAGGA AGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACAGTGTGGG TATCAAGAGC CAATGTGATC 
CAGCGCCGGG GCCGGGCGGG CCGCTGCCAG TCCGGCTTTG CCTACCACTT GTTCCCTCGA 
AGCCGGCTGG AGAAAATGGT CCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCGAA AATCCACATG CCTGAGAAGA CGGCGGTGGA GTTCCTGTCC 
AAGGCTGTGG ACAGTCCAAA CATCAAGGCA GTGGACGAGG CTGTGATCTT GCTCCAGGAG 
ATCGGGGTGC TGGACCAGCG GGAGTACCTG ACTACCCTGG GGCAGCGCCT GGCTCACATC 
TCCACCGACC CCCGGTTGGC CAAGGCCATT GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTACTGG TGGTCGTTTC CTGCCTCACC CGGGACCCCT TCAGCAGCAG CCTACAGAAC 
CGGGCAGAGG TGGACAAGGT GAAAGCACTG TTGAGCCATG ACAGCGGCAG TGACCACCTG 
GCCTTTGTGC GGGCTGTCGC CGGCTGGGAG GAGGTGCTGC GTTGGCAGGA CCGCAGCTCC 
CGGGAGAATT ACCTGGAGGA AAACCTGCTG TACGCACCCA GCCTGCGCTT CATCCACGGA 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCCTTCC TGGTGGGGAA GCCCTCGGAC 
TGCACCCTGG CCTCCGCCCA GTGCAACGAG TACAGTGAGG AGGAGGAGCT GGTGAAGGGC 
GTGCTGATGG CCGGCCTCTA CCCCAACCTC ATCCAGGTGA GGCAGGGCAA GGTCACCCGG 
CAGGGGAAGT TCAAGCCCAA CAGCGTCACA TATAGGACCA AATCAGGCAA CATCCTGCTG 
CACAAGTCGA CCATTAACAG GGAGGCCACA CGGTTACGGA GCCGATGGCT GACGTATTTC 
ATGGCAGTCA AGTCCAATGG CAGCGTCTTC GTCCGGGACT CCTCTCAGGT GCACCCGCTA 
GCTGTGCTGC TGCTGACCGA CGGGGACGTG CACATCCGTG ATGACGGGCG CCGGGCCACC 
ATCTCACTGA GCGACAGTGA CCTGCTGCGG CTGGAGGGTG ACTCGCGTAC CGTGCGGCTG 
CTGAAGGAGC TGCGGCGGGC CCTGGGCCGC ATGGTGGAGC GGAGCCTGCG CAGCGAGCTG 
GCTGCACTTC CCCCCAGCGT ACAGGAGGAG CACGGGCAGC TGCTTGCGCT ACTGGCAGAG 
CTGCTGCGAG GACCCTGTGG CAGCTTTGAT GTGCGCAAGA CAGCTGACGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_017005915.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861404.1 Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X9, mRNA.

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CloneID	OHu37057
Clone ID Related Accession (Same CDS sequence)	XM_017005915.1 , XM_006713033.1
Accession Version	XM_017005915.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX30 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCC TGGACTCATT CAGAAAAGCT TCTAGGGACC TATTAAAAGA GTTCCCACAG 
CCCAAAAATC TTCTCAACAG TGTGATTGGA AGAGCCCTCG GCATCTCACA TGCAAAAGAC 
AAACTAGTCT ACGTGCACAC AAATGGACCG AAGAAAAAGA AAGTCACACT GCACATAAAA 
TGGCCCAAGA GCGTGGAGGT AGAAGGCTAT GGCAGCAAGA AGATCGATGC TGAGCGGCAG 
GCTGCAGCTG CAGCCTGCCA GCTGTTCAAG GGTTGGGGTC TGCTAGGTCC CCGGAATGAG 
TTGTTTGACG CAGCCAAATA CCGAGTGCTA GCTGATCGCT TTGGCTCCCC TGCCGACAGC 
TGGTGGCGTC CGGAACCCAC CATGCCCCCT ACTTCCTGGC GGCAGCTGAA TCCAGAGAGT 
ATTCGACCAG GGGGACCTGG GGGCCTATCC CGCTCTTTAG GCCGGGAAGA AGAGGAGGAC 
GAGGAGGAAG AGCTAGAAGA AGGGACCATA GATGTTACCG ACTTCTTGTC CATGACCCAG 
CAGGATTCCC ACGCTCCACT CAGGGACTCA AGGGGGAGTT CCTTTGAGAT GACAGATGAC 
GACAGTGCCA TTAGGGCTCT GACCCAGTTT CCACTTCCCA AGAACCTTCT GGCCAAGGTG 
ATTCAGATTG CAACGTCATC CTCCACAGCT AAGAACCTCA TGCAGTTCCA TACTGTGGGC 
ACCAAGACCA AGCTGTCTAC ACTCACCCTG CTCTGGCCCT GCCCCATGAC CTTTGTTGCC 
AAAGGGCGCC GCAAAGCAGA GGCTGAGAAT AAGGCGGCAG CCTTGGCCTG CAAGAAACTG 
AAGAGCCTGG GCCTGGTGGA CAGGAACAAC GAACCGCTTA CACACGCCAT GTATAACCTG 
GCCTCTTTGC GTGAGCTGGG TGAGACCCAG CGCCGACCAT GCACCATCCA GGTGCCCGAG 
CCCATCCTCC GCAAGATAGA GACCTTCCTG AACCATTACC CTGTGGAGAG TTCATGGATC 
GCCCCAGAAC TCCGGCTGCA GAGTGATGAC ATCTTGCCCT TGGGCAAGGA CTCAGGGCCT 
CTGAGTGACC CTATCACAGG CAAGCCCTAT GTGCCCCTGT TGGAAGCAGA GGAGGTACGT 
CTCAGCCAGA GTCTGCTAGA ACTGTGGCGG CGGCGAGGGC CGGTCTGGCA GGAGGCCCCC 
CAGCTACCTG TGGACCCACA TCGGGACACC ATCCTCAACG CCATTGAGCA GCACCCGGTG 
GTGGTCATCT CTGGGGACAC GGGCTGTGGG AAGACCACGC GCATCCCCCA GCTGTTGCTG 
GAGCGCTATG TGACCGAGGG CCGAGGTGCC CGCTGCAATG TTATCATCAC CCAACCTCGC 
CGCATCTCTG CTGTGTCTGT GGCACAGCGG GTCAGCCACG AACTGGGCCC CTCCCTGCGC 
CGGAATGTGG GCTTCCAGGT GCGGTTGGAA AGTAAGCCCC CATCCCGAGG CGGGGCCCTG 
CTCTTCTGCA CTGTGGGTAT CCTGCTGCGT AAGCTGCAGA GCAACCCCAG CCTGGAGGGC 
GTGAGCCACG TCATCGTGGA TGAGGTGCAT GAGCGGGACG TGAACACAGA CTTTCTGCTG 
ATCCTGCTCA AGGGCCTGCA GCGGCTCAAC CCGGCCCTGC GGCTGGTGCT CATGAGTGCC 
ACAGGGGACA ATGAGCGCTT CTCCCGATAC TTTGGTGGCT GCCCCGTCAT CAAGGTGCCT 
GGCTTCATGT ACCCAGTCAA GGAGCACTAC CTAGAGGACA TCCTGGCCAA GTTGGGCAAG 
CACCAGTACC TGCACCGGCA CCGGCACCAT GAGTCTGAGG ATGAATGCGC ACTCGATTTG 
GACCTTGTGA CTGATCTGGT TCTGCACATC GATGCTCGCG GGGAACCAGG TGGGATCCTG 
TGCTTCCTGC CTGGGTGGCA GGAGATCAAA GGAGTGCAGC AGCGCCTCCA GGAGGCCCTG 
GGCATGCACG AGAGCAAGTA CCTCATCCTG CCAGTGCACT CCAACATCCC CATGATGGAT 
CAGAAGGCCA TATTCCAGCA GCCTCCAGTT GGGGTGCGCA AGATTGTCTT GGCCACCAAC 
ATTGCTGAGA CTTCCATCAC AATCAATGAC ATCGTGCATG TGGTGGACAG TGGGCTGCAC 
AAGGAAGAAC GCTATGACCT GAAGACCAAG GTGTCCTGCC TGGAGACAGT GTGGGTATCA 
AGAGCCAATG TGATCCAGCG CCGGGGCCGG GCGGGCCGCT GCCAGTCCGG CTTTGCCTAC 
CACTTGTTCC CTCGAAGCCG GCTGGAGAAA ATGGTCCCTT TCCAAGTGCC AGAGATCCTG 
CGCACACCTC TTGAGAACCT GGTGCTGCAA GCGAAAATCC ACATGCCTGA GAAGACGGCG 
GTGGAGTTCC TGTCCAAGGC TGTGGACAGT CCAAACATCA AGGCAGTGGA CGAGGCTGTG 
ATCTTGCTCC AGGAGATCGG GGTGCTGGAC CAGCGGGAGT ACCTGACTAC CCTGGGGCAG 
CGCCTGGCTC ACATCTCCAC CGACCCCCGG TTGGCCAAGG CCATTGTGTT GGCTGCCATC 
TTCCGTTGCC TGCACCCACT ACTGGTGGTC GTTTCCTGCC TCACCCGGGA CCCCTTCAGC 
AGCAGCCTAC AGAACCGGGC AGAGGTGGAC AAGGTGAAAG CACTGTTGAG CCATGACAGC 
GGCAGTGACC ACCTGGCCTT TGTGCGGGCT GTCGCCGGCT GGGAGGAGGT GCTGCGTTGG 
CAGGACCGCA GCTCCCGGGA GAATTACCTG GAGGAAAACC TGCTGTACGC ACCCAGCCTG 
CGCTTCATCC ACGGACTCAT CAAGCAGTTC TCAGAGAACA TTTATGAGGC CTTCCTGGTG 
GGGAAGCCCT CGGACTGCAC CCTGGCCTCC GCCCAGTGCA ACGAGTACAG TGAGGAGGAG 
GAGCTGGTGA AGGGCGTGCT GATGGCCGGC CTCTACCCCA ACCTCATCCA GGTGAGGCAG 
GGCAAGGTCA CCCGGCAGGG GAAGTTCAAG CCCAACAGCG TCACATATAG GACCAAATCA 
GGCAACATCC TGCTGCACAA GTCGACCATT AACAGGGAGG CCACACGGTT ACGGAGCCGA 
TGGCTGACGT ATTTCATGGC AGTCAAGTCC AATGGCAGCG TCTTCGTCCG GGACTCCTCT 
CAGGTGCACC CGCTAGCTGT GCTGCTGCTG ACCGACGGGG ACGTGCACAT CCGTGATGAC 
GGGCGCCGGG CCACCATCTC ACTGAGCGAC AGTGACCTGC TGCGGCTGGA GGGTGACTCG 
CGTACCGTGC GGCTGCTGAA GGAGCTGCGG CGGGCCCTGG GCCGCATGGT GGAGCGGAGC 
CTGCGCAGCG AGCTGGCTGC ACTTCCCCCC AGCGTACAGG AGGAGCACGG GCAGCTGCTT 
GCGCTACTGG CAGAGCTGCT GCGAGGACCC TGTGGCAGCT TTGATGTGCG CAAGACAGCT 
GACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861404.1
CDS	303..3791
Translation	MFSLDSFRKASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_017005915.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005915.1

ORF Insert Sequence:

ATGTTCAGCC TGGACTCATT CAGAAAAGCT TCTAGGGACC TATTAAAAGA GTTCCCACAG 
CCCAAAAATC TTCTCAACAG TGTGATTGGA AGAGCCCTCG GCATCTCACA TGCAAAAGAC 
AAACTAGTCT ACGTGCACAC AAATGGACCG AAGAAAAAGA AAGTCACACT GCACATAAAA 
TGGCCCAAGA GCGTGGAGGT AGAAGGCTAT GGCAGCAAGA AGATCGATGC TGAGCGGCAG 
GCTGCAGCTG CAGCCTGCCA GCTGTTCAAG GGTTGGGGTC TGCTAGGTCC CCGGAATGAG 
TTGTTTGACG CAGCCAAATA CCGAGTGCTA GCTGATCGCT TTGGCTCCCC TGCCGACAGC 
TGGTGGCGTC CGGAACCCAC CATGCCCCCT ACTTCCTGGC GGCAGCTGAA TCCAGAGAGT 
ATTCGACCAG GGGGACCTGG GGGCCTATCC CGCTCTTTAG GCCGGGAAGA AGAGGAGGAC 
GAGGAGGAAG AGCTAGAAGA AGGGACCATA GATGTTACCG ACTTCTTGTC CATGACCCAG 
CAGGATTCCC ACGCTCCACT CAGGGACTCA AGGGGGAGTT CCTTTGAGAT GACAGATGAC 
GACAGTGCCA TTAGGGCTCT GACCCAGTTT CCACTTCCCA AGAACCTTCT GGCCAAGGTG 
ATTCAGATTG CAACGTCATC CTCCACAGCT AAGAACCTCA TGCAGTTCCA TACTGTGGGC 
ACCAAGACCA AGCTGTCTAC ACTCACCCTG CTCTGGCCCT GCCCCATGAC CTTTGTTGCC 
AAAGGGCGCC GCAAAGCAGA GGCTGAGAAT AAGGCGGCAG CCTTGGCCTG CAAGAAACTG 
AAGAGCCTGG GCCTGGTGGA CAGGAACAAC GAACCGCTTA CACACGCCAT GTATAACCTG 
GCCTCTTTGC GTGAGCTGGG TGAGACCCAG CGCCGACCAT GCACCATCCA GGTGCCCGAG 
CCCATCCTCC GCAAGATAGA GACCTTCCTG AACCATTACC CTGTGGAGAG TTCATGGATC 
GCCCCAGAAC TCCGGCTGCA GAGTGATGAC ATCTTGCCCT TGGGCAAGGA CTCAGGGCCT 
CTGAGTGACC CTATCACAGG CAAGCCCTAT GTGCCCCTGT TGGAAGCAGA GGAGGTACGT 
CTCAGCCAGA GTCTGCTAGA ACTGTGGCGG CGGCGAGGGC CGGTCTGGCA GGAGGCCCCC 
CAGCTACCTG TGGACCCACA TCGGGACACC ATCCTCAACG CCATTGAGCA GCACCCGGTG 
GTGGTCATCT CTGGGGACAC GGGCTGTGGG AAGACCACGC GCATCCCCCA GCTGTTGCTG 
GAGCGCTATG TGACCGAGGG CCGAGGTGCC CGCTGCAATG TTATCATCAC CCAACCTCGC 
CGCATCTCTG CTGTGTCTGT GGCACAGCGG GTCAGCCACG AACTGGGCCC CTCCCTGCGC 
CGGAATGTGG GCTTCCAGGT GCGGTTGGAA AGTAAGCCCC CATCCCGAGG CGGGGCCCTG 
CTCTTCTGCA CTGTGGGTAT CCTGCTGCGT AAGCTGCAGA GCAACCCCAG CCTGGAGGGC 
GTGAGCCACG TCATCGTGGA TGAGGTGCAT GAGCGGGACG TGAACACAGA CTTTCTGCTG 
ATCCTGCTCA AGGGCCTGCA GCGGCTCAAC CCGGCCCTGC GGCTGGTGCT CATGAGTGCC 
ACAGGGGACA ATGAGCGCTT CTCCCGATAC TTTGGTGGCT GCCCCGTCAT CAAGGTGCCT 
GGCTTCATGT ACCCAGTCAA GGAGCACTAC CTAGAGGACA TCCTGGCCAA GTTGGGCAAG 
CACCAGTACC TGCACCGGCA CCGGCACCAT GAGTCTGAGG ATGAATGCGC ACTCGATTTG 
GACCTTGTGA CTGATCTGGT TCTGCACATC GATGCTCGCG GGGAACCAGG TGGGATCCTG 
TGCTTCCTGC CTGGGTGGCA GGAGATCAAA GGAGTGCAGC AGCGCCTCCA GGAGGCCCTG 
GGCATGCACG AGAGCAAGTA CCTCATCCTG CCAGTGCACT CCAACATCCC CATGATGGAT 
CAGAAGGCCA TATTCCAGCA GCCTCCAGTT GGGGTGCGCA AGATTGTCTT GGCCACCAAC 
ATTGCTGAGA CTTCCATCAC AATCAATGAC ATCGTGCATG TGGTGGACAG TGGGCTGCAC 
AAGGAAGAAC GCTATGACCT GAAGACCAAG GTGTCCTGCC TGGAGACAGT GTGGGTATCA 
AGAGCCAATG TGATCCAGCG CCGGGGCCGG GCGGGCCGCT GCCAGTCCGG CTTTGCCTAC 
CACTTGTTCC CTCGAAGCCG GCTGGAGAAA ATGGTCCCTT TCCAAGTGCC AGAGATCCTG 
CGCACACCTC TTGAGAACCT GGTGCTGCAA GCGAAAATCC ACATGCCTGA GAAGACGGCG 
GTGGAGTTCC TGTCCAAGGC TGTGGACAGT CCAAACATCA AGGCAGTGGA CGAGGCTGTG 
ATCTTGCTCC AGGAGATCGG GGTGCTGGAC CAGCGGGAGT ACCTGACTAC CCTGGGGCAG 
CGCCTGGCTC ACATCTCCAC CGACCCCCGG TTGGCCAAGG CCATTGTGTT GGCTGCCATC 
TTCCGTTGCC TGCACCCACT ACTGGTGGTC GTTTCCTGCC TCACCCGGGA CCCCTTCAGC 
AGCAGCCTAC AGAACCGGGC AGAGGTGGAC AAGGTGAAAG CACTGTTGAG CCATGACAGC 
GGCAGTGACC ACCTGGCCTT TGTGCGGGCT GTCGCCGGCT GGGAGGAGGT GCTGCGTTGG 
CAGGACCGCA GCTCCCGGGA GAATTACCTG GAGGAAAACC TGCTGTACGC ACCCAGCCTG 
CGCTTCATCC ACGGACTCAT CAAGCAGTTC TCAGAGAACA TTTATGAGGC CTTCCTGGTG 
GGGAAGCCCT CGGACTGCAC CCTGGCCTCC GCCCAGTGCA ACGAGTACAG TGAGGAGGAG 
GAGCTGGTGA AGGGCGTGCT GATGGCCGGC CTCTACCCCA ACCTCATCCA GGTGAGGCAG 
GGCAAGGTCA CCCGGCAGGG GAAGTTCAAG CCCAACAGCG TCACATATAG GACCAAATCA 
GGCAACATCC TGCTGCACAA GTCGACCATT AACAGGGAGG CCACACGGTT ACGGAGCCGA 
TGGCTGACGT ATTTCATGGC AGTCAAGTCC AATGGCAGCG TCTTCGTCCG GGACTCCTCT 
CAGGTGCACC CGCTAGCTGT GCTGCTGCTG ACCGACGGGG ACGTGCACAT CCGTGATGAC 
GGGCGCCGGG CCACCATCTC ACTGAGCGAC AGTGACCTGC TGCGGCTGGA GGGTGACTCG 
CGTACCGTGC GGCTGCTGAA GGAGCTGCGG CGGGCCCTGG GCCGCATGGT GGAGCGGAGC 
CTGCGCAGCG AGCTGGCTGC ACTTCCCCCC AGCGTACAGG AGGAGCACGG GCAGCTGCTT 
GCGCTACTGG CAGAGCTGCT GCGAGGACCC TGTGGCAGCT TTGATGTGCG CAAGACAGCT 
GACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_006713033.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713096.1 Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X8, mRNA.

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CloneID	OHu37057
Clone ID Related Accession (Same CDS sequence)	XM_017005915.1 , XM_006713033.1
Accession Version	XM_006713033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX30 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCC TGGACTCATT CAGAAAAGCT TCTAGGGACC TATTAAAAGA GTTCCCACAG 
CCCAAAAATC TTCTCAACAG TGTGATTGGA AGAGCCCTCG GCATCTCACA TGCAAAAGAC 
AAACTAGTCT ACGTGCACAC AAATGGACCG AAGAAAAAGA AAGTCACACT GCACATAAAA 
TGGCCCAAGA GCGTGGAGGT AGAAGGCTAT GGCAGCAAGA AGATCGATGC TGAGCGGCAG 
GCTGCAGCTG CAGCCTGCCA GCTGTTCAAG GGTTGGGGTC TGCTAGGTCC CCGGAATGAG 
TTGTTTGACG CAGCCAAATA CCGAGTGCTA GCTGATCGCT TTGGCTCCCC TGCCGACAGC 
TGGTGGCGTC CGGAACCCAC CATGCCCCCT ACTTCCTGGC GGCAGCTGAA TCCAGAGAGT 
ATTCGACCAG GGGGACCTGG GGGCCTATCC CGCTCTTTAG GCCGGGAAGA AGAGGAGGAC 
GAGGAGGAAG AGCTAGAAGA AGGGACCATA GATGTTACCG ACTTCTTGTC CATGACCCAG 
CAGGATTCCC ACGCTCCACT CAGGGACTCA AGGGGGAGTT CCTTTGAGAT GACAGATGAC 
GACAGTGCCA TTAGGGCTCT GACCCAGTTT CCACTTCCCA AGAACCTTCT GGCCAAGGTG 
ATTCAGATTG CAACGTCATC CTCCACAGCT AAGAACCTCA TGCAGTTCCA TACTGTGGGC 
ACCAAGACCA AGCTGTCTAC ACTCACCCTG CTCTGGCCCT GCCCCATGAC CTTTGTTGCC 
AAAGGGCGCC GCAAAGCAGA GGCTGAGAAT AAGGCGGCAG CCTTGGCCTG CAAGAAACTG 
AAGAGCCTGG GCCTGGTGGA CAGGAACAAC GAACCGCTTA CACACGCCAT GTATAACCTG 
GCCTCTTTGC GTGAGCTGGG TGAGACCCAG CGCCGACCAT GCACCATCCA GGTGCCCGAG 
CCCATCCTCC GCAAGATAGA GACCTTCCTG AACCATTACC CTGTGGAGAG TTCATGGATC 
GCCCCAGAAC TCCGGCTGCA GAGTGATGAC ATCTTGCCCT TGGGCAAGGA CTCAGGGCCT 
CTGAGTGACC CTATCACAGG CAAGCCCTAT GTGCCCCTGT TGGAAGCAGA GGAGGTACGT 
CTCAGCCAGA GTCTGCTAGA ACTGTGGCGG CGGCGAGGGC CGGTCTGGCA GGAGGCCCCC 
CAGCTACCTG TGGACCCACA TCGGGACACC ATCCTCAACG CCATTGAGCA GCACCCGGTG 
GTGGTCATCT CTGGGGACAC GGGCTGTGGG AAGACCACGC GCATCCCCCA GCTGTTGCTG 
GAGCGCTATG TGACCGAGGG CCGAGGTGCC CGCTGCAATG TTATCATCAC CCAACCTCGC 
CGCATCTCTG CTGTGTCTGT GGCACAGCGG GTCAGCCACG AACTGGGCCC CTCCCTGCGC 
CGGAATGTGG GCTTCCAGGT GCGGTTGGAA AGTAAGCCCC CATCCCGAGG CGGGGCCCTG 
CTCTTCTGCA CTGTGGGTAT CCTGCTGCGT AAGCTGCAGA GCAACCCCAG CCTGGAGGGC 
GTGAGCCACG TCATCGTGGA TGAGGTGCAT GAGCGGGACG TGAACACAGA CTTTCTGCTG 
ATCCTGCTCA AGGGCCTGCA GCGGCTCAAC CCGGCCCTGC GGCTGGTGCT CATGAGTGCC 
ACAGGGGACA ATGAGCGCTT CTCCCGATAC TTTGGTGGCT GCCCCGTCAT CAAGGTGCCT 
GGCTTCATGT ACCCAGTCAA GGAGCACTAC CTAGAGGACA TCCTGGCCAA GTTGGGCAAG 
CACCAGTACC TGCACCGGCA CCGGCACCAT GAGTCTGAGG ATGAATGCGC ACTCGATTTG 
GACCTTGTGA CTGATCTGGT TCTGCACATC GATGCTCGCG GGGAACCAGG TGGGATCCTG 
TGCTTCCTGC CTGGGTGGCA GGAGATCAAA GGAGTGCAGC AGCGCCTCCA GGAGGCCCTG 
GGCATGCACG AGAGCAAGTA CCTCATCCTG CCAGTGCACT CCAACATCCC CATGATGGAT 
CAGAAGGCCA TATTCCAGCA GCCTCCAGTT GGGGTGCGCA AGATTGTCTT GGCCACCAAC 
ATTGCTGAGA CTTCCATCAC AATCAATGAC ATCGTGCATG TGGTGGACAG TGGGCTGCAC 
AAGGAAGAAC GCTATGACCT GAAGACCAAG GTGTCCTGCC TGGAGACAGT GTGGGTATCA 
AGAGCCAATG TGATCCAGCG CCGGGGCCGG GCGGGCCGCT GCCAGTCCGG CTTTGCCTAC 
CACTTGTTCC CTCGAAGCCG GCTGGAGAAA ATGGTCCCTT TCCAAGTGCC AGAGATCCTG 
CGCACACCTC TTGAGAACCT GGTGCTGCAA GCGAAAATCC ACATGCCTGA GAAGACGGCG 
GTGGAGTTCC TGTCCAAGGC TGTGGACAGT CCAAACATCA AGGCAGTGGA CGAGGCTGTG 
ATCTTGCTCC AGGAGATCGG GGTGCTGGAC CAGCGGGAGT ACCTGACTAC CCTGGGGCAG 
CGCCTGGCTC ACATCTCCAC CGACCCCCGG TTGGCCAAGG CCATTGTGTT GGCTGCCATC 
TTCCGTTGCC TGCACCCACT ACTGGTGGTC GTTTCCTGCC TCACCCGGGA CCCCTTCAGC 
AGCAGCCTAC AGAACCGGGC AGAGGTGGAC AAGGTGAAAG CACTGTTGAG CCATGACAGC 
GGCAGTGACC ACCTGGCCTT TGTGCGGGCT GTCGCCGGCT GGGAGGAGGT GCTGCGTTGG 
CAGGACCGCA GCTCCCGGGA GAATTACCTG GAGGAAAACC TGCTGTACGC ACCCAGCCTG 
CGCTTCATCC ACGGACTCAT CAAGCAGTTC TCAGAGAACA TTTATGAGGC CTTCCTGGTG 
GGGAAGCCCT CGGACTGCAC CCTGGCCTCC GCCCAGTGCA ACGAGTACAG TGAGGAGGAG 
GAGCTGGTGA AGGGCGTGCT GATGGCCGGC CTCTACCCCA ACCTCATCCA GGTGAGGCAG 
GGCAAGGTCA CCCGGCAGGG GAAGTTCAAG CCCAACAGCG TCACATATAG GACCAAATCA 
GGCAACATCC TGCTGCACAA GTCGACCATT AACAGGGAGG CCACACGGTT ACGGAGCCGA 
TGGCTGACGT ATTTCATGGC AGTCAAGTCC AATGGCAGCG TCTTCGTCCG GGACTCCTCT 
CAGGTGCACC CGCTAGCTGT GCTGCTGCTG ACCGACGGGG ACGTGCACAT CCGTGATGAC 
GGGCGCCGGG CCACCATCTC ACTGAGCGAC AGTGACCTGC TGCGGCTGGA GGGTGACTCG 
CGTACCGTGC GGCTGCTGAA GGAGCTGCGG CGGGCCCTGG GCCGCATGGT GGAGCGGAGC 
CTGCGCAGCG AGCTGGCTGC ACTTCCCCCC AGCGTACAGG AGGAGCACGG GCAGCTGCTT 
GCGCTACTGG CAGAGCTGCT GCGAGGACCC TGTGGCAGCT TTGATGTGCG CAAGACAGCT 
GACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713096.1
CDS	346..3834
Translation	MFSLDSFRKASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_006713033.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713033.1

ORF Insert Sequence:

ATGTTCAGCC TGGACTCATT CAGAAAAGCT TCTAGGGACC TATTAAAAGA GTTCCCACAG 
CCCAAAAATC TTCTCAACAG TGTGATTGGA AGAGCCCTCG GCATCTCACA TGCAAAAGAC 
AAACTAGTCT ACGTGCACAC AAATGGACCG AAGAAAAAGA AAGTCACACT GCACATAAAA 
TGGCCCAAGA GCGTGGAGGT AGAAGGCTAT GGCAGCAAGA AGATCGATGC TGAGCGGCAG 
GCTGCAGCTG CAGCCTGCCA GCTGTTCAAG GGTTGGGGTC TGCTAGGTCC CCGGAATGAG 
TTGTTTGACG CAGCCAAATA CCGAGTGCTA GCTGATCGCT TTGGCTCCCC TGCCGACAGC 
TGGTGGCGTC CGGAACCCAC CATGCCCCCT ACTTCCTGGC GGCAGCTGAA TCCAGAGAGT 
ATTCGACCAG GGGGACCTGG GGGCCTATCC CGCTCTTTAG GCCGGGAAGA AGAGGAGGAC 
GAGGAGGAAG AGCTAGAAGA AGGGACCATA GATGTTACCG ACTTCTTGTC CATGACCCAG 
CAGGATTCCC ACGCTCCACT CAGGGACTCA AGGGGGAGTT CCTTTGAGAT GACAGATGAC 
GACAGTGCCA TTAGGGCTCT GACCCAGTTT CCACTTCCCA AGAACCTTCT GGCCAAGGTG 
ATTCAGATTG CAACGTCATC CTCCACAGCT AAGAACCTCA TGCAGTTCCA TACTGTGGGC 
ACCAAGACCA AGCTGTCTAC ACTCACCCTG CTCTGGCCCT GCCCCATGAC CTTTGTTGCC 
AAAGGGCGCC GCAAAGCAGA GGCTGAGAAT AAGGCGGCAG CCTTGGCCTG CAAGAAACTG 
AAGAGCCTGG GCCTGGTGGA CAGGAACAAC GAACCGCTTA CACACGCCAT GTATAACCTG 
GCCTCTTTGC GTGAGCTGGG TGAGACCCAG CGCCGACCAT GCACCATCCA GGTGCCCGAG 
CCCATCCTCC GCAAGATAGA GACCTTCCTG AACCATTACC CTGTGGAGAG TTCATGGATC 
GCCCCAGAAC TCCGGCTGCA GAGTGATGAC ATCTTGCCCT TGGGCAAGGA CTCAGGGCCT 
CTGAGTGACC CTATCACAGG CAAGCCCTAT GTGCCCCTGT TGGAAGCAGA GGAGGTACGT 
CTCAGCCAGA GTCTGCTAGA ACTGTGGCGG CGGCGAGGGC CGGTCTGGCA GGAGGCCCCC 
CAGCTACCTG TGGACCCACA TCGGGACACC ATCCTCAACG CCATTGAGCA GCACCCGGTG 
GTGGTCATCT CTGGGGACAC GGGCTGTGGG AAGACCACGC GCATCCCCCA GCTGTTGCTG 
GAGCGCTATG TGACCGAGGG CCGAGGTGCC CGCTGCAATG TTATCATCAC CCAACCTCGC 
CGCATCTCTG CTGTGTCTGT GGCACAGCGG GTCAGCCACG AACTGGGCCC CTCCCTGCGC 
CGGAATGTGG GCTTCCAGGT GCGGTTGGAA AGTAAGCCCC CATCCCGAGG CGGGGCCCTG 
CTCTTCTGCA CTGTGGGTAT CCTGCTGCGT AAGCTGCAGA GCAACCCCAG CCTGGAGGGC 
GTGAGCCACG TCATCGTGGA TGAGGTGCAT GAGCGGGACG TGAACACAGA CTTTCTGCTG 
ATCCTGCTCA AGGGCCTGCA GCGGCTCAAC CCGGCCCTGC GGCTGGTGCT CATGAGTGCC 
ACAGGGGACA ATGAGCGCTT CTCCCGATAC TTTGGTGGCT GCCCCGTCAT CAAGGTGCCT 
GGCTTCATGT ACCCAGTCAA GGAGCACTAC CTAGAGGACA TCCTGGCCAA GTTGGGCAAG 
CACCAGTACC TGCACCGGCA CCGGCACCAT GAGTCTGAGG ATGAATGCGC ACTCGATTTG 
GACCTTGTGA CTGATCTGGT TCTGCACATC GATGCTCGCG GGGAACCAGG TGGGATCCTG 
TGCTTCCTGC CTGGGTGGCA GGAGATCAAA GGAGTGCAGC AGCGCCTCCA GGAGGCCCTG 
GGCATGCACG AGAGCAAGTA CCTCATCCTG CCAGTGCACT CCAACATCCC CATGATGGAT 
CAGAAGGCCA TATTCCAGCA GCCTCCAGTT GGGGTGCGCA AGATTGTCTT GGCCACCAAC 
ATTGCTGAGA CTTCCATCAC AATCAATGAC ATCGTGCATG TGGTGGACAG TGGGCTGCAC 
AAGGAAGAAC GCTATGACCT GAAGACCAAG GTGTCCTGCC TGGAGACAGT GTGGGTATCA 
AGAGCCAATG TGATCCAGCG CCGGGGCCGG GCGGGCCGCT GCCAGTCCGG CTTTGCCTAC 
CACTTGTTCC CTCGAAGCCG GCTGGAGAAA ATGGTCCCTT TCCAAGTGCC AGAGATCCTG 
CGCACACCTC TTGAGAACCT GGTGCTGCAA GCGAAAATCC ACATGCCTGA GAAGACGGCG 
GTGGAGTTCC TGTCCAAGGC TGTGGACAGT CCAAACATCA AGGCAGTGGA CGAGGCTGTG 
ATCTTGCTCC AGGAGATCGG GGTGCTGGAC CAGCGGGAGT ACCTGACTAC CCTGGGGCAG 
CGCCTGGCTC ACATCTCCAC CGACCCCCGG TTGGCCAAGG CCATTGTGTT GGCTGCCATC 
TTCCGTTGCC TGCACCCACT ACTGGTGGTC GTTTCCTGCC TCACCCGGGA CCCCTTCAGC 
AGCAGCCTAC AGAACCGGGC AGAGGTGGAC AAGGTGAAAG CACTGTTGAG CCATGACAGC 
GGCAGTGACC ACCTGGCCTT TGTGCGGGCT GTCGCCGGCT GGGAGGAGGT GCTGCGTTGG 
CAGGACCGCA GCTCCCGGGA GAATTACCTG GAGGAAAACC TGCTGTACGC ACCCAGCCTG 
CGCTTCATCC ACGGACTCAT CAAGCAGTTC TCAGAGAACA TTTATGAGGC CTTCCTGGTG 
GGGAAGCCCT CGGACTGCAC CCTGGCCTCC GCCCAGTGCA ACGAGTACAG TGAGGAGGAG 
GAGCTGGTGA AGGGCGTGCT GATGGCCGGC CTCTACCCCA ACCTCATCCA GGTGAGGCAG 
GGCAAGGTCA CCCGGCAGGG GAAGTTCAAG CCCAACAGCG TCACATATAG GACCAAATCA 
GGCAACATCC TGCTGCACAA GTCGACCATT AACAGGGAGG CCACACGGTT ACGGAGCCGA 
TGGCTGACGT ATTTCATGGC AGTCAAGTCC AATGGCAGCG TCTTCGTCCG GGACTCCTCT 
CAGGTGCACC CGCTAGCTGT GCTGCTGCTG ACCGACGGGG ACGTGCACAT CCGTGATGAC 
GGGCGCCGGG CCACCATCTC ACTGAGCGAC AGTGACCTGC TGCGGCTGGA GGGTGACTCG 
CGTACCGTGC GGCTGCTGAA GGAGCTGCGG CGGGCCCTGG GCCGCATGGT GGAGCGGAGC 
CTGCGCAGCG AGCTGGCTGC ACTTCCCCCC AGCGTACAGG AGGAGCACGG GCAGCTGCTT 
GCGCTACTGG CAGAGCTGCT GCGAGGACCC TGTGGCAGCT TTGATGTGCG CAAGACAGCT 
GACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_011533490.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531792.1 Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X1, mRNA.

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CloneID	OHu65125
Clone ID Related Accession (Same CDS sequence)	XM_011533490.1 , XM_011533490.2 , XM_011533492.2 , XM_011533491.1
Accession Version	XM_011533490.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGC CTAGGCTTGG GCTTCGGGTC CGCCCGGCCC GCAGGGGGCG CGCGGCGCTC 
GGGCCGGCCG CTCCCGTTCT CTTCGCCCGG TCCCTGCCGC GCACAGGCCT CGGGGTCGGC 
GGGAGCACGA TGGCGGCCGC TAGGAGACTC ATGGCGCTGG CCGCCGGCAT CTCTCCGCGC 
CTGCAGCCGC TGGGTCCCCG CGCTGCTGGG CGACAGGGTC GCTCGCGCGG CTTCTCTTCA 
AGCTGCGCCC ACCCCGACCA CACCAAGGAA GCCGCCGAGG CCGAGTCAGG GATGGCCCCC 
GGCGGGCCTG GGGAAGGCGA CGGAAGCTTG GTGAACGCTT CTAGGGACCT ATTAAAAGAG 
TTCCCACAGC CCAAAAATCT TCTCAACAGT GTGATTGGAA GAGCCCTCGG CATCTCACAT 
GCAAAAGACA AACTAGTCTA CGTGCACACA AATGGACCGA AGAAAAAGAA AGTCACACTG 
CACATAAAAT GGCCCAAGAG CGTGGAGGTA GAAGGCTATG GCAGCAAGAA GATCGATGCT 
GAGCGGCAGG CTGCAGCTGC AGCCTGCCAG CTGTTCAAGG GTTGGGGTCT GCTAGGTCCC 
CGGAATGAGT TGTTTGACGC AGCCAAATAC CGAGTGCTAG CTGATCGCTT TGGCTCCCCT 
GCCGACAGCT GGTGGCGTCC GGAACCCACC ATGCCCCCTA CTTCCTGGCG GCAGCTGAAT 
CCAGAGAGTA TTCGACCAGG GGGACCTGGG GGCCTATCCC GCTCTTTAGG CCGGGAAGAA 
GAGGAGGACG AGGAGGAAGA GCTAGAAGAA GGGACCATAG ATGTTACCGA CTTCTTGTCC 
ATGACCCAGC AGGATTCCCA CGCTCCACTC AGGGACTCAA GGGGGAGTTC CTTTGAGATG 
ACAGATGACG ACAGTGCCAT TAGGGCTCTG ACCCAGTTTC CACTTCCCAA GAACCTTCTG 
GCCAAGGTGA TTCAGATTGC AACGTCATCC TCCACAGCTA AGAACCTCAT GCAGTTCCAT 
ACTGTGGGCA CCAAGACCAA GCTGTCTACA CTCACCCTGC TCTGGCCCTG CCCCATGACC 
TTTGTTGCCA AAGGGCGCCG CAAAGCAGAG GCTGAGAATA AGGCGGCAGC CTTGGCCTGC 
AAGAAACTGA AGAGCCTGGG CCTGGTGGAC AGGAACAACG AACCGCTTAC ACACGCCATG 
TATAACCTGG CCTCTTTGCG TGAGCTGGGT GAGACCCAGC GCCGACCATG CACCATCCAG 
GTGCCCGAGC CCATCCTCCG CAAGATAGAG ACCTTCCTGA ACCATTACCC TGTGGAGAGT 
TCATGGATCG CCCCAGAACT CCGGCTGCAG AGTGATGACA TCTTGCCCTT GGGCAAGGAC 
TCAGGGCCTC TGAGTGACCC TATCACAGGC AAGCCCTATG TGCCCCTGTT GGAAGCAGAG 
GAGGTACGTC TCAGCCAGAG TCTGCTAGAA CTGTGGCGGC GGCGAGGGCC GGTCTGGCAG 
GAGGCCCCCC AGCTACCTGT GGACCCACAT CGGGACACCA TCCTCAACGC CATTGAGCAG 
CACCCGGTGG TGGTCATCTC TGGGGACACG GGCTGTGGGA AGACCACGCG CATCCCCCAG 
CTGTTGCTGG AGCGCTATGT GACCGAGGGC CGAGGTGCCC GCTGCAATGT TATCATCACC 
CAACCTCGCC GCATCTCTGC TGTGTCTGTG GCACAGCGGG TCAGCCACGA ACTGGGCCCC 
TCCCTGCGCC GGAATGTGGG CTTCCAGGTG CGGTTGGAAA GTAAGCCCCC ATCCCGAGGC 
GGGGCCCTGC TCTTCTGCAC TGTGGGTATC CTGCTGCGTA AGCTGCAGAG CAACCCCAGC 
CTGGAGGGCG TGAGCCACGT CATCGTGGAT GAGGTGCATG AGCGGGACGT GAACACAGAC 
TTTCTGCTGA TCCTGCTCAA GGGCCTGCAG CGGCTCAACC CGGCCCTGCG GCTGGTGCTC 
ATGAGTGCCA CAGGGGACAA TGAGCGCTTC TCCCGATACT TTGGTGGCTG CCCCGTCATC 
AAGGTGCCTG GCTTCATGTA CCCAGTCAAG GAGCACTACC TAGAGGACAT CCTGGCCAAG 
TTGGGCAAGC ACCAGTACCT GCACCGGCAC CGGCACCATG AGTCTGAGGA TGAATGCGCA 
CTCGATTTGG ACCTTGTGAC TGATCTGGTT CTGCACATCG ATGCTCGCGG GGAACCAGGT 
GGGATCCTGT GCTTCCTGCC TGGGTGGCAG GAGATCAAAG GAGTGCAGCA GCGCCTCCAG 
GAGGCCCTGG GCATGCACGA GAGCAAGTAC CTCATCCTGC CAGTGCACTC CAACATCCCC 
ATGATGGATC AGAAGGCCAT ATTCCAGCAG CCTCCAGTTG GGGTGCGCAA GATTGTCTTG 
GCCACCAACA TTGCTGAGAC TTCCATCACA ATCAATGACA TCGTGCATGT GGTGGACAGT 
GGGCTGCACA AGGAAGAACG CTATGACCTG AAGACCAAGG TGTCCTGCCT GGAGACAGTG 
TGGGTATCAA GAGCCAATGT GATCCAGCGC CGGGGCCGGG CGGGCCGCTG CCAGTCCGGC 
TTTGCCTACC ACTTGTTCCC TCGAAGCCGG CTGGAGAAAA TGGTCCCTTT CCAAGTGCCA 
GAGATCCTGC GCACACCTCT TGAGAACCTG GTGCTGCAAG CGAAAATCCA CATGCCTGAG 
AAGACGGCGG TGGAGTTCCT GTCCAAGGCT GTGGACAGTC CAAACATCAA GGCAGTGGAC 
GAGGCTGTGA TCTTGCTCCA GGAGATCGGG GTGCTGGACC AGCGGGAGTA CCTGACTACC 
CTGGGGCAGC GCCTGGCTCA CATCTCCACC GACCCCCGGT TGGCCAAGGC CATTGTGTTG 
GCTGCCATCT TCCGTTGCCT GCACCCACTA CTGGTGGTCG TTTCCTGCCT CACCCGGGAC 
CCCTTCAGCA GCAGCCTACA GAACCGGGCA GAGGTGGACA AGGTGAAAGC ACTGTTGAGC 
CATGACAGCG GCAGTGACCA CCTGGCCTTT GTGCGGGCTG TCGCCGGCTG GGAGGAGGTG 
CTGCGTTGGC AGGACCGCAG CTCCCGGGAG AATTACCTGG AGGAAAACCT GCTGTACGCA 
CCCAGCCTGC GCTTCATCCA CGGACTCATC AAGCAGTTCT CAGAGAACAT TTATGAGGCC 
TTCCTGGTGG GGAAGCCCTC GGACTGCACC CTGGCCTCCG CCCAGTGCAA CGAGTACAGT 
GAGGAGGAGG AGCTGGTGAA GGGCGTGCTG ATGGCCGGCC TCTACCCCAA CCTCATCCAG 
GTGAGGCAGG GCAAGGTCAC CCGGCAGGGG AAGTTCAAGC CCAACAGCGT CACATATAGG 
ACCAAATCAG GCAACATCCT GCTGCACAAG TCGACCATTA ACAGGGAGGC CACACGGTTA 
CGGAGCCGAT GGCTGACGTA TTTCATGGCA GTCAAGTCCA ATGGCAGCGT CTTCGTCCGG 
GACTCCTCTC AGGTGCACCC GCTAGCTGTG CTGCTGCTGA CCGACGGGGA CGTGCACATC 
CGTGATGACG GGCGCCGGGC CACCATCTCA CTGAGCGACA GTGACCTGCT GCGGCTGGAG 
GGTGACTCGC GTACCGTGCG GCTGCTGAAG GAGCTGCGGC GGGCCCTGGG CCGCATGGTG 
GAGCGGAGCC TGCGCAGCGA GCTGGCTGCA CTTCCCCCCA GCGTACAGGA GGAGCACGGG 
CAGCTGCTTG CGCTACTGGC AGAGCTGCTG CGAGGACCCT GTGGCAGCTT TGATGTGCGC 
AAGACAGCTG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531792.1
CDS	469..4266
Translation	MPSPRLGLRVRPARRGRAALGPAAPVLFARSLPRTGLGVGGSTMAAARRLMALAAGISPRLQPLGPRAAGRQGRSRGFSSSCAHPDHTKEAAEAESGMAPGGPGEGDGSLVNASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_011533490.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533490.1

ORF Insert Sequence:

ATGCCCTCGC CTAGGCTTGG GCTTCGGGTC CGCCCGGCCC GCAGGGGGCG CGCGGCGCTC 
GGGCCGGCCG CTCCCGTTCT CTTCGCCCGG TCCCTGCCGC GCACAGGCCT CGGGGTCGGC 
GGGAGCACGA TGGCGGCCGC TAGGAGACTC ATGGCGCTGG CCGCCGGCAT CTCTCCGCGC 
CTGCAGCCGC TGGGTCCCCG CGCTGCTGGG CGACAGGGTC GCTCGCGCGG CTTCTCTTCA 
AGCTGCGCCC ACCCCGACCA CACCAAGGAA GCCGCCGAGG CCGAGTCAGG GATGGCCCCC 
GGCGGGCCTG GGGAAGGCGA CGGAAGCTTG GTGAACGCTT CTAGGGACCT ATTAAAAGAG 
TTCCCACAGC CCAAAAATCT TCTCAACAGT GTGATTGGAA GAGCCCTCGG CATCTCACAT 
GCAAAAGACA AACTAGTCTA CGTGCACACA AATGGACCGA AGAAAAAGAA AGTCACACTG 
CACATAAAAT GGCCCAAGAG CGTGGAGGTA GAAGGCTATG GCAGCAAGAA GATCGATGCT 
GAGCGGCAGG CTGCAGCTGC AGCCTGCCAG CTGTTCAAGG GTTGGGGTCT GCTAGGTCCC 
CGGAATGAGT TGTTTGACGC AGCCAAATAC CGAGTGCTAG CTGATCGCTT TGGCTCCCCT 
GCCGACAGCT GGTGGCGTCC GGAACCCACC ATGCCCCCTA CTTCCTGGCG GCAGCTGAAT 
CCAGAGAGTA TTCGACCAGG GGGACCTGGG GGCCTATCCC GCTCTTTAGG CCGGGAAGAA 
GAGGAGGACG AGGAGGAAGA GCTAGAAGAA GGGACCATAG ATGTTACCGA CTTCTTGTCC 
ATGACCCAGC AGGATTCCCA CGCTCCACTC AGGGACTCAA GGGGGAGTTC CTTTGAGATG 
ACAGATGACG ACAGTGCCAT TAGGGCTCTG ACCCAGTTTC CACTTCCCAA GAACCTTCTG 
GCCAAGGTGA TTCAGATTGC AACGTCATCC TCCACAGCTA AGAACCTCAT GCAGTTCCAT 
ACTGTGGGCA CCAAGACCAA GCTGTCTACA CTCACCCTGC TCTGGCCCTG CCCCATGACC 
TTTGTTGCCA AAGGGCGCCG CAAAGCAGAG GCTGAGAATA AGGCGGCAGC CTTGGCCTGC 
AAGAAACTGA AGAGCCTGGG CCTGGTGGAC AGGAACAACG AACCGCTTAC ACACGCCATG 
TATAACCTGG CCTCTTTGCG TGAGCTGGGT GAGACCCAGC GCCGACCATG CACCATCCAG 
GTGCCCGAGC CCATCCTCCG CAAGATAGAG ACCTTCCTGA ACCATTACCC TGTGGAGAGT 
TCATGGATCG CCCCAGAACT CCGGCTGCAG AGTGATGACA TCTTGCCCTT GGGCAAGGAC 
TCAGGGCCTC TGAGTGACCC TATCACAGGC AAGCCCTATG TGCCCCTGTT GGAAGCAGAG 
GAGGTACGTC TCAGCCAGAG TCTGCTAGAA CTGTGGCGGC GGCGAGGGCC GGTCTGGCAG 
GAGGCCCCCC AGCTACCTGT GGACCCACAT CGGGACACCA TCCTCAACGC CATTGAGCAG 
CACCCGGTGG TGGTCATCTC TGGGGACACG GGCTGTGGGA AGACCACGCG CATCCCCCAG 
CTGTTGCTGG AGCGCTATGT GACCGAGGGC CGAGGTGCCC GCTGCAATGT TATCATCACC 
CAACCTCGCC GCATCTCTGC TGTGTCTGTG GCACAGCGGG TCAGCCACGA ACTGGGCCCC 
TCCCTGCGCC GGAATGTGGG CTTCCAGGTG CGGTTGGAAA GTAAGCCCCC ATCCCGAGGC 
GGGGCCCTGC TCTTCTGCAC TGTGGGTATC CTGCTGCGTA AGCTGCAGAG CAACCCCAGC 
CTGGAGGGCG TGAGCCACGT CATCGTGGAT GAGGTGCATG AGCGGGACGT GAACACAGAC 
TTTCTGCTGA TCCTGCTCAA GGGCCTGCAG CGGCTCAACC CGGCCCTGCG GCTGGTGCTC 
ATGAGTGCCA CAGGGGACAA TGAGCGCTTC TCCCGATACT TTGGTGGCTG CCCCGTCATC 
AAGGTGCCTG GCTTCATGTA CCCAGTCAAG GAGCACTACC TAGAGGACAT CCTGGCCAAG 
TTGGGCAAGC ACCAGTACCT GCACCGGCAC CGGCACCATG AGTCTGAGGA TGAATGCGCA 
CTCGATTTGG ACCTTGTGAC TGATCTGGTT CTGCACATCG ATGCTCGCGG GGAACCAGGT 
GGGATCCTGT GCTTCCTGCC TGGGTGGCAG GAGATCAAAG GAGTGCAGCA GCGCCTCCAG 
GAGGCCCTGG GCATGCACGA GAGCAAGTAC CTCATCCTGC CAGTGCACTC CAACATCCCC 
ATGATGGATC AGAAGGCCAT ATTCCAGCAG CCTCCAGTTG GGGTGCGCAA GATTGTCTTG 
GCCACCAACA TTGCTGAGAC TTCCATCACA ATCAATGACA TCGTGCATGT GGTGGACAGT 
GGGCTGCACA AGGAAGAACG CTATGACCTG AAGACCAAGG TGTCCTGCCT GGAGACAGTG 
TGGGTATCAA GAGCCAATGT GATCCAGCGC CGGGGCCGGG CGGGCCGCTG CCAGTCCGGC 
TTTGCCTACC ACTTGTTCCC TCGAAGCCGG CTGGAGAAAA TGGTCCCTTT CCAAGTGCCA 
GAGATCCTGC GCACACCTCT TGAGAACCTG GTGCTGCAAG CGAAAATCCA CATGCCTGAG 
AAGACGGCGG TGGAGTTCCT GTCCAAGGCT GTGGACAGTC CAAACATCAA GGCAGTGGAC 
GAGGCTGTGA TCTTGCTCCA GGAGATCGGG GTGCTGGACC AGCGGGAGTA CCTGACTACC 
CTGGGGCAGC GCCTGGCTCA CATCTCCACC GACCCCCGGT TGGCCAAGGC CATTGTGTTG 
GCTGCCATCT TCCGTTGCCT GCACCCACTA CTGGTGGTCG TTTCCTGCCT CACCCGGGAC 
CCCTTCAGCA GCAGCCTACA GAACCGGGCA GAGGTGGACA AGGTGAAAGC ACTGTTGAGC 
CATGACAGCG GCAGTGACCA CCTGGCCTTT GTGCGGGCTG TCGCCGGCTG GGAGGAGGTG 
CTGCGTTGGC AGGACCGCAG CTCCCGGGAG AATTACCTGG AGGAAAACCT GCTGTACGCA 
CCCAGCCTGC GCTTCATCCA CGGACTCATC AAGCAGTTCT CAGAGAACAT TTATGAGGCC 
TTCCTGGTGG GGAAGCCCTC GGACTGCACC CTGGCCTCCG CCCAGTGCAA CGAGTACAGT 
GAGGAGGAGG AGCTGGTGAA GGGCGTGCTG ATGGCCGGCC TCTACCCCAA CCTCATCCAG 
GTGAGGCAGG GCAAGGTCAC CCGGCAGGGG AAGTTCAAGC CCAACAGCGT CACATATAGG 
ACCAAATCAG GCAACATCCT GCTGCACAAG TCGACCATTA ACAGGGAGGC CACACGGTTA 
CGGAGCCGAT GGCTGACGTA TTTCATGGCA GTCAAGTCCA ATGGCAGCGT CTTCGTCCGG 
GACTCCTCTC AGGTGCACCC GCTAGCTGTG CTGCTGCTGA CCGACGGGGA CGTGCACATC 
CGTGATGACG GGCGCCGGGC CACCATCTCA CTGAGCGACA GTGACCTGCT GCGGCTGGAG 
GGTGACTCGC GTACCGTGCG GCTGCTGAAG GAGCTGCGGC GGGCCCTGGG CCGCATGGTG 
GAGCGGAGCC TGCGCAGCGA GCTGGCTGCA CTTCCCCCCA GCGTACAGGA GGAGCACGGG 
CAGCTGCTTG CGCTACTGGC AGAGCTGCTG CGAGGACCCT GTGGCAGCTT TGATGTGCGC 
AAGACAGCTG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_011533490.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531792.1 Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X1, mRNA.

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CloneID	OHu65125
Clone ID Related Accession (Same CDS sequence)	XM_011533490.1 , XM_011533490.2 , XM_011533492.2 , XM_011533491.1
Accession Version	XM_011533490.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011533490.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGC CTAGGCTTGG GCTTCGGGTC CGCCCGGCCC GCAGGGGGCG CGCGGCGCTC 
GGGCCGGCCG CTCCCGTTCT CTTCGCCCGG TCCCTGCCGC GCACAGGCCT CGGGGTCGGC 
GGGAGCACGA TGGCGGCCGC TAGGAGACTC ATGGCGCTGG CCGCCGGCAT CTCTCCGCGC 
CTGCAGCCGC TGGGTCCCCG CGCTGCTGGG CGACAGGGTC GCTCGCGCGG CTTCTCTTCA 
AGCTGCGCCC ACCCCGACCA CACCAAGGAA GCCGCCGAGG CCGAGTCAGG GATGGCCCCC 
GGCGGGCCTG GGGAAGGCGA CGGAAGCTTG GTGAACGCTT CTAGGGACCT ATTAAAAGAG 
TTCCCACAGC CCAAAAATCT TCTCAACAGT GTGATTGGAA GAGCCCTCGG CATCTCACAT 
GCAAAAGACA AACTAGTCTA CGTGCACACA AATGGACCGA AGAAAAAGAA AGTCACACTG 
CACATAAAAT GGCCCAAGAG CGTGGAGGTA GAAGGCTATG GCAGCAAGAA GATCGATGCT 
GAGCGGCAGG CTGCAGCTGC AGCCTGCCAG CTGTTCAAGG GTTGGGGTCT GCTAGGTCCC 
CGGAATGAGT TGTTTGACGC AGCCAAATAC CGAGTGCTAG CTGATCGCTT TGGCTCCCCT 
GCCGACAGCT GGTGGCGTCC GGAACCCACC ATGCCCCCTA CTTCCTGGCG GCAGCTGAAT 
CCAGAGAGTA TTCGACCAGG GGGACCTGGG GGCCTATCCC GCTCTTTAGG CCGGGAAGAA 
GAGGAGGACG AGGAGGAAGA GCTAGAAGAA GGGACCATAG ATGTTACCGA CTTCTTGTCC 
ATGACCCAGC AGGATTCCCA CGCTCCACTC AGGGACTCAA GGGGGAGTTC CTTTGAGATG 
ACAGATGACG ACAGTGCCAT TAGGGCTCTG ACCCAGTTTC CACTTCCCAA GAACCTTCTG 
GCCAAGGTGA TTCAGATTGC AACGTCATCC TCCACAGCTA AGAACCTCAT GCAGTTCCAT 
ACTGTGGGCA CCAAGACCAA GCTGTCTACA CTCACCCTGC TCTGGCCCTG CCCCATGACC 
TTTGTTGCCA AAGGGCGCCG CAAAGCAGAG GCTGAGAATA AGGCGGCAGC CTTGGCCTGC 
AAGAAACTGA AGAGCCTGGG CCTGGTGGAC AGGAACAACG AACCGCTTAC ACACGCCATG 
TATAACCTGG CCTCTTTGCG TGAGCTGGGT GAGACCCAGC GCCGACCATG CACCATCCAG 
GTGCCCGAGC CCATCCTCCG CAAGATAGAG ACCTTCCTGA ACCATTACCC TGTGGAGAGT 
TCATGGATCG CCCCAGAACT CCGGCTGCAG AGTGATGACA TCTTGCCCTT GGGCAAGGAC 
TCAGGGCCTC TGAGTGACCC TATCACAGGC AAGCCCTATG TGCCCCTGTT GGAAGCAGAG 
GAGGTACGTC TCAGCCAGAG TCTGCTAGAA CTGTGGCGGC GGCGAGGGCC GGTCTGGCAG 
GAGGCCCCCC AGCTACCTGT GGACCCACAT CGGGACACCA TCCTCAACGC CATTGAGCAG 
CACCCGGTGG TGGTCATCTC TGGGGACACG GGCTGTGGGA AGACCACGCG CATCCCCCAG 
CTGTTGCTGG AGCGCTATGT GACCGAGGGC CGAGGTGCCC GCTGCAATGT TATCATCACC 
CAACCTCGCC GCATCTCTGC TGTGTCTGTG GCACAGCGGG TCAGCCACGA ACTGGGCCCC 
TCCCTGCGCC GGAATGTGGG CTTCCAGGTG CGGTTGGAAA GTAAGCCCCC ATCCCGAGGC 
GGGGCCCTGC TCTTCTGCAC TGTGGGTATC CTGCTGCGTA AGCTGCAGAG CAACCCCAGC 
CTGGAGGGCG TGAGCCACGT CATCGTGGAT GAGGTGCATG AGCGGGACGT GAACACAGAC 
TTTCTGCTGA TCCTGCTCAA GGGCCTGCAG CGGCTCAACC CGGCCCTGCG GCTGGTGCTC 
ATGAGTGCCA CAGGGGACAA TGAGCGCTTC TCCCGATACT TTGGTGGCTG CCCCGTCATC 
AAGGTGCCTG GCTTCATGTA CCCAGTCAAG GAGCACTACC TAGAGGACAT CCTGGCCAAG 
TTGGGCAAGC ACCAGTACCT GCACCGGCAC CGGCACCATG AGTCTGAGGA TGAATGCGCA 
CTCGATTTGG ACCTTGTGAC TGATCTGGTT CTGCACATCG ATGCTCGCGG GGAACCAGGT 
GGGATCCTGT GCTTCCTGCC TGGGTGGCAG GAGATCAAAG GAGTGCAGCA GCGCCTCCAG 
GAGGCCCTGG GCATGCACGA GAGCAAGTAC CTCATCCTGC CAGTGCACTC CAACATCCCC 
ATGATGGATC AGAAGGCCAT ATTCCAGCAG CCTCCAGTTG GGGTGCGCAA GATTGTCTTG 
GCCACCAACA TTGCTGAGAC TTCCATCACA ATCAATGACA TCGTGCATGT GGTGGACAGT 
GGGCTGCACA AGGAAGAACG CTATGACCTG AAGACCAAGG TGTCCTGCCT GGAGACAGTG 
TGGGTATCAA GAGCCAATGT GATCCAGCGC CGGGGCCGGG CGGGCCGCTG CCAGTCCGGC 
TTTGCCTACC ACTTGTTCCC TCGAAGCCGG CTGGAGAAAA TGGTCCCTTT CCAAGTGCCA 
GAGATCCTGC GCACACCTCT TGAGAACCTG GTGCTGCAAG CGAAAATCCA CATGCCTGAG 
AAGACGGCGG TGGAGTTCCT GTCCAAGGCT GTGGACAGTC CAAACATCAA GGCAGTGGAC 
GAGGCTGTGA TCTTGCTCCA GGAGATCGGG GTGCTGGACC AGCGGGAGTA CCTGACTACC 
CTGGGGCAGC GCCTGGCTCA CATCTCCACC GACCCCCGGT TGGCCAAGGC CATTGTGTTG 
GCTGCCATCT TCCGTTGCCT GCACCCACTA CTGGTGGTCG TTTCCTGCCT CACCCGGGAC 
CCCTTCAGCA GCAGCCTACA GAACCGGGCA GAGGTGGACA AGGTGAAAGC ACTGTTGAGC 
CATGACAGCG GCAGTGACCA CCTGGCCTTT GTGCGGGCTG TCGCCGGCTG GGAGGAGGTG 
CTGCGTTGGC AGGACCGCAG CTCCCGGGAG AATTACCTGG AGGAAAACCT GCTGTACGCA 
CCCAGCCTGC GCTTCATCCA CGGACTCATC AAGCAGTTCT CAGAGAACAT TTATGAGGCC 
TTCCTGGTGG GGAAGCCCTC GGACTGCACC CTGGCCTCCG CCCAGTGCAA CGAGTACAGT 
GAGGAGGAGG AGCTGGTGAA GGGCGTGCTG ATGGCCGGCC TCTACCCCAA CCTCATCCAG 
GTGAGGCAGG GCAAGGTCAC CCGGCAGGGG AAGTTCAAGC CCAACAGCGT CACATATAGG 
ACCAAATCAG GCAACATCCT GCTGCACAAG TCGACCATTA ACAGGGAGGC CACACGGTTA 
CGGAGCCGAT GGCTGACGTA TTTCATGGCA GTCAAGTCCA ATGGCAGCGT CTTCGTCCGG 
GACTCCTCTC AGGTGCACCC GCTAGCTGTG CTGCTGCTGA CCGACGGGGA CGTGCACATC 
CGTGATGACG GGCGCCGGGC CACCATCTCA CTGAGCGACA GTGACCTGCT GCGGCTGGAG 
GGTGACTCGC GTACCGTGCG GCTGCTGAAG GAGCTGCGGC GGGCCCTGGG CCGCATGGTG 
GAGCGGAGCC TGCGCAGCGA GCTGGCTGCA CTTCCCCCCA GCGTACAGGA GGAGCACGGG 
CAGCTGCTTG CGCTACTGGC AGAGCTGCTG CGAGGACCCT GTGGCAGCTT TGATGTGCGC 
AAGACAGCTG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531792.1
CDS	517..4314
Translation	MPSPRLGLRVRPARRGRAALGPAAPVLFARSLPRTGLGVGGSTMAAARRLMALAAGISPRLQPLGPRAAGRQGRSRGFSSSCAHPDHTKEAAEAESGMAPGGPGEGDGSLVNASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_011533490.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533490.2

ORF Insert Sequence:

ATGCCCTCGC CTAGGCTTGG GCTTCGGGTC CGCCCGGCCC GCAGGGGGCG CGCGGCGCTC 
GGGCCGGCCG CTCCCGTTCT CTTCGCCCGG TCCCTGCCGC GCACAGGCCT CGGGGTCGGC 
GGGAGCACGA TGGCGGCCGC TAGGAGACTC ATGGCGCTGG CCGCCGGCAT CTCTCCGCGC 
CTGCAGCCGC TGGGTCCCCG CGCTGCTGGG CGACAGGGTC GCTCGCGCGG CTTCTCTTCA 
AGCTGCGCCC ACCCCGACCA CACCAAGGAA GCCGCCGAGG CCGAGTCAGG GATGGCCCCC 
GGCGGGCCTG GGGAAGGCGA CGGAAGCTTG GTGAACGCTT CTAGGGACCT ATTAAAAGAG 
TTCCCACAGC CCAAAAATCT TCTCAACAGT GTGATTGGAA GAGCCCTCGG CATCTCACAT 
GCAAAAGACA AACTAGTCTA CGTGCACACA AATGGACCGA AGAAAAAGAA AGTCACACTG 
CACATAAAAT GGCCCAAGAG CGTGGAGGTA GAAGGCTATG GCAGCAAGAA GATCGATGCT 
GAGCGGCAGG CTGCAGCTGC AGCCTGCCAG CTGTTCAAGG GTTGGGGTCT GCTAGGTCCC 
CGGAATGAGT TGTTTGACGC AGCCAAATAC CGAGTGCTAG CTGATCGCTT TGGCTCCCCT 
GCCGACAGCT GGTGGCGTCC GGAACCCACC ATGCCCCCTA CTTCCTGGCG GCAGCTGAAT 
CCAGAGAGTA TTCGACCAGG GGGACCTGGG GGCCTATCCC GCTCTTTAGG CCGGGAAGAA 
GAGGAGGACG AGGAGGAAGA GCTAGAAGAA GGGACCATAG ATGTTACCGA CTTCTTGTCC 
ATGACCCAGC AGGATTCCCA CGCTCCACTC AGGGACTCAA GGGGGAGTTC CTTTGAGATG 
ACAGATGACG ACAGTGCCAT TAGGGCTCTG ACCCAGTTTC CACTTCCCAA GAACCTTCTG 
GCCAAGGTGA TTCAGATTGC AACGTCATCC TCCACAGCTA AGAACCTCAT GCAGTTCCAT 
ACTGTGGGCA CCAAGACCAA GCTGTCTACA CTCACCCTGC TCTGGCCCTG CCCCATGACC 
TTTGTTGCCA AAGGGCGCCG CAAAGCAGAG GCTGAGAATA AGGCGGCAGC CTTGGCCTGC 
AAGAAACTGA AGAGCCTGGG CCTGGTGGAC AGGAACAACG AACCGCTTAC ACACGCCATG 
TATAACCTGG CCTCTTTGCG TGAGCTGGGT GAGACCCAGC GCCGACCATG CACCATCCAG 
GTGCCCGAGC CCATCCTCCG CAAGATAGAG ACCTTCCTGA ACCATTACCC TGTGGAGAGT 
TCATGGATCG CCCCAGAACT CCGGCTGCAG AGTGATGACA TCTTGCCCTT GGGCAAGGAC 
TCAGGGCCTC TGAGTGACCC TATCACAGGC AAGCCCTATG TGCCCCTGTT GGAAGCAGAG 
GAGGTACGTC TCAGCCAGAG TCTGCTAGAA CTGTGGCGGC GGCGAGGGCC GGTCTGGCAG 
GAGGCCCCCC AGCTACCTGT GGACCCACAT CGGGACACCA TCCTCAACGC CATTGAGCAG 
CACCCGGTGG TGGTCATCTC TGGGGACACG GGCTGTGGGA AGACCACGCG CATCCCCCAG 
CTGTTGCTGG AGCGCTATGT GACCGAGGGC CGAGGTGCCC GCTGCAATGT TATCATCACC 
CAACCTCGCC GCATCTCTGC TGTGTCTGTG GCACAGCGGG TCAGCCACGA ACTGGGCCCC 
TCCCTGCGCC GGAATGTGGG CTTCCAGGTG CGGTTGGAAA GTAAGCCCCC ATCCCGAGGC 
GGGGCCCTGC TCTTCTGCAC TGTGGGTATC CTGCTGCGTA AGCTGCAGAG CAACCCCAGC 
CTGGAGGGCG TGAGCCACGT CATCGTGGAT GAGGTGCATG AGCGGGACGT GAACACAGAC 
TTTCTGCTGA TCCTGCTCAA GGGCCTGCAG CGGCTCAACC CGGCCCTGCG GCTGGTGCTC 
ATGAGTGCCA CAGGGGACAA TGAGCGCTTC TCCCGATACT TTGGTGGCTG CCCCGTCATC 
AAGGTGCCTG GCTTCATGTA CCCAGTCAAG GAGCACTACC TAGAGGACAT CCTGGCCAAG 
TTGGGCAAGC ACCAGTACCT GCACCGGCAC CGGCACCATG AGTCTGAGGA TGAATGCGCA 
CTCGATTTGG ACCTTGTGAC TGATCTGGTT CTGCACATCG ATGCTCGCGG GGAACCAGGT 
GGGATCCTGT GCTTCCTGCC TGGGTGGCAG GAGATCAAAG GAGTGCAGCA GCGCCTCCAG 
GAGGCCCTGG GCATGCACGA GAGCAAGTAC CTCATCCTGC CAGTGCACTC CAACATCCCC 
ATGATGGATC AGAAGGCCAT ATTCCAGCAG CCTCCAGTTG GGGTGCGCAA GATTGTCTTG 
GCCACCAACA TTGCTGAGAC TTCCATCACA ATCAATGACA TCGTGCATGT GGTGGACAGT 
GGGCTGCACA AGGAAGAACG CTATGACCTG AAGACCAAGG TGTCCTGCCT GGAGACAGTG 
TGGGTATCAA GAGCCAATGT GATCCAGCGC CGGGGCCGGG CGGGCCGCTG CCAGTCCGGC 
TTTGCCTACC ACTTGTTCCC TCGAAGCCGG CTGGAGAAAA TGGTCCCTTT CCAAGTGCCA 
GAGATCCTGC GCACACCTCT TGAGAACCTG GTGCTGCAAG CGAAAATCCA CATGCCTGAG 
AAGACGGCGG TGGAGTTCCT GTCCAAGGCT GTGGACAGTC CAAACATCAA GGCAGTGGAC 
GAGGCTGTGA TCTTGCTCCA GGAGATCGGG GTGCTGGACC AGCGGGAGTA CCTGACTACC 
CTGGGGCAGC GCCTGGCTCA CATCTCCACC GACCCCCGGT TGGCCAAGGC CATTGTGTTG 
GCTGCCATCT TCCGTTGCCT GCACCCACTA CTGGTGGTCG TTTCCTGCCT CACCCGGGAC 
CCCTTCAGCA GCAGCCTACA GAACCGGGCA GAGGTGGACA AGGTGAAAGC ACTGTTGAGC 
CATGACAGCG GCAGTGACCA CCTGGCCTTT GTGCGGGCTG TCGCCGGCTG GGAGGAGGTG 
CTGCGTTGGC AGGACCGCAG CTCCCGGGAG AATTACCTGG AGGAAAACCT GCTGTACGCA 
CCCAGCCTGC GCTTCATCCA CGGACTCATC AAGCAGTTCT CAGAGAACAT TTATGAGGCC 
TTCCTGGTGG GGAAGCCCTC GGACTGCACC CTGGCCTCCG CCCAGTGCAA CGAGTACAGT 
GAGGAGGAGG AGCTGGTGAA GGGCGTGCTG ATGGCCGGCC TCTACCCCAA CCTCATCCAG 
GTGAGGCAGG GCAAGGTCAC CCGGCAGGGG AAGTTCAAGC CCAACAGCGT CACATATAGG 
ACCAAATCAG GCAACATCCT GCTGCACAAG TCGACCATTA ACAGGGAGGC CACACGGTTA 
CGGAGCCGAT GGCTGACGTA TTTCATGGCA GTCAAGTCCA ATGGCAGCGT CTTCGTCCGG 
GACTCCTCTC AGGTGCACCC GCTAGCTGTG CTGCTGCTGA CCGACGGGGA CGTGCACATC 
CGTGATGACG GGCGCCGGGC CACCATCTCA CTGAGCGACA GTGACCTGCT GCGGCTGGAG 
GGTGACTCGC GTACCGTGCG GCTGCTGAAG GAGCTGCGGC GGGCCCTGGG CCGCATGGTG 
GAGCGGAGCC TGCGCAGCGA GCTGGCTGCA CTTCCCCCCA GCGTACAGGA GGAGCACGGG 
CAGCTGCTTG CGCTACTGGC AGAGCTGCTG CGAGGACCCT GTGGCAGCTT TGATGTGCGC 
AAGACAGCTG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_011533492.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531794.1 Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu65125
Clone ID Related Accession (Same CDS sequence)	XM_011533490.1 , XM_011533490.2 , XM_011533492.2 , XM_011533491.1
Accession Version	XM_011533492.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533492.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGC CTAGGCTTGG GCTTCGGGTC CGCCCGGCCC GCAGGGGGCG CGCGGCGCTC 
GGGCCGGCCG CTCCCGTTCT CTTCGCCCGG TCCCTGCCGC GCACAGGCCT CGGGGTCGGC 
GGGAGCACGA TGGCGGCCGC TAGGAGACTC ATGGCGCTGG CCGCCGGCAT CTCTCCGCGC 
CTGCAGCCGC TGGGTCCCCG CGCTGCTGGG CGACAGGGTC GCTCGCGCGG CTTCTCTTCA 
AGCTGCGCCC ACCCCGACCA CACCAAGGAA GCCGCCGAGG CCGAGTCAGG GATGGCCCCC 
GGCGGGCCTG GGGAAGGCGA CGGAAGCTTG GTGAACGCTT CTAGGGACCT ATTAAAAGAG 
TTCCCACAGC CCAAAAATCT TCTCAACAGT GTGATTGGAA GAGCCCTCGG CATCTCACAT 
GCAAAAGACA AACTAGTCTA CGTGCACACA AATGGACCGA AGAAAAAGAA AGTCACACTG 
CACATAAAAT GGCCCAAGAG CGTGGAGGTA GAAGGCTATG GCAGCAAGAA GATCGATGCT 
GAGCGGCAGG CTGCAGCTGC AGCCTGCCAG CTGTTCAAGG GTTGGGGTCT GCTAGGTCCC 
CGGAATGAGT TGTTTGACGC AGCCAAATAC CGAGTGCTAG CTGATCGCTT TGGCTCCCCT 
GCCGACAGCT GGTGGCGTCC GGAACCCACC ATGCCCCCTA CTTCCTGGCG GCAGCTGAAT 
CCAGAGAGTA TTCGACCAGG GGGACCTGGG GGCCTATCCC GCTCTTTAGG CCGGGAAGAA 
GAGGAGGACG AGGAGGAAGA GCTAGAAGAA GGGACCATAG ATGTTACCGA CTTCTTGTCC 
ATGACCCAGC AGGATTCCCA CGCTCCACTC AGGGACTCAA GGGGGAGTTC CTTTGAGATG 
ACAGATGACG ACAGTGCCAT TAGGGCTCTG ACCCAGTTTC CACTTCCCAA GAACCTTCTG 
GCCAAGGTGA TTCAGATTGC AACGTCATCC TCCACAGCTA AGAACCTCAT GCAGTTCCAT 
ACTGTGGGCA CCAAGACCAA GCTGTCTACA CTCACCCTGC TCTGGCCCTG CCCCATGACC 
TTTGTTGCCA AAGGGCGCCG CAAAGCAGAG GCTGAGAATA AGGCGGCAGC CTTGGCCTGC 
AAGAAACTGA AGAGCCTGGG CCTGGTGGAC AGGAACAACG AACCGCTTAC ACACGCCATG 
TATAACCTGG CCTCTTTGCG TGAGCTGGGT GAGACCCAGC GCCGACCATG CACCATCCAG 
GTGCCCGAGC CCATCCTCCG CAAGATAGAG ACCTTCCTGA ACCATTACCC TGTGGAGAGT 
TCATGGATCG CCCCAGAACT CCGGCTGCAG AGTGATGACA TCTTGCCCTT GGGCAAGGAC 
TCAGGGCCTC TGAGTGACCC TATCACAGGC AAGCCCTATG TGCCCCTGTT GGAAGCAGAG 
GAGGTACGTC TCAGCCAGAG TCTGCTAGAA CTGTGGCGGC GGCGAGGGCC GGTCTGGCAG 
GAGGCCCCCC AGCTACCTGT GGACCCACAT CGGGACACCA TCCTCAACGC CATTGAGCAG 
CACCCGGTGG TGGTCATCTC TGGGGACACG GGCTGTGGGA AGACCACGCG CATCCCCCAG 
CTGTTGCTGG AGCGCTATGT GACCGAGGGC CGAGGTGCCC GCTGCAATGT TATCATCACC 
CAACCTCGCC GCATCTCTGC TGTGTCTGTG GCACAGCGGG TCAGCCACGA ACTGGGCCCC 
TCCCTGCGCC GGAATGTGGG CTTCCAGGTG CGGTTGGAAA GTAAGCCCCC ATCCCGAGGC 
GGGGCCCTGC TCTTCTGCAC TGTGGGTATC CTGCTGCGTA AGCTGCAGAG CAACCCCAGC 
CTGGAGGGCG TGAGCCACGT CATCGTGGAT GAGGTGCATG AGCGGGACGT GAACACAGAC 
TTTCTGCTGA TCCTGCTCAA GGGCCTGCAG CGGCTCAACC CGGCCCTGCG GCTGGTGCTC 
ATGAGTGCCA CAGGGGACAA TGAGCGCTTC TCCCGATACT TTGGTGGCTG CCCCGTCATC 
AAGGTGCCTG GCTTCATGTA CCCAGTCAAG GAGCACTACC TAGAGGACAT CCTGGCCAAG 
TTGGGCAAGC ACCAGTACCT GCACCGGCAC CGGCACCATG AGTCTGAGGA TGAATGCGCA 
CTCGATTTGG ACCTTGTGAC TGATCTGGTT CTGCACATCG ATGCTCGCGG GGAACCAGGT 
GGGATCCTGT GCTTCCTGCC TGGGTGGCAG GAGATCAAAG GAGTGCAGCA GCGCCTCCAG 
GAGGCCCTGG GCATGCACGA GAGCAAGTAC CTCATCCTGC CAGTGCACTC CAACATCCCC 
ATGATGGATC AGAAGGCCAT ATTCCAGCAG CCTCCAGTTG GGGTGCGCAA GATTGTCTTG 
GCCACCAACA TTGCTGAGAC TTCCATCACA ATCAATGACA TCGTGCATGT GGTGGACAGT 
GGGCTGCACA AGGAAGAACG CTATGACCTG AAGACCAAGG TGTCCTGCCT GGAGACAGTG 
TGGGTATCAA GAGCCAATGT GATCCAGCGC CGGGGCCGGG CGGGCCGCTG CCAGTCCGGC 
TTTGCCTACC ACTTGTTCCC TCGAAGCCGG CTGGAGAAAA TGGTCCCTTT CCAAGTGCCA 
GAGATCCTGC GCACACCTCT TGAGAACCTG GTGCTGCAAG CGAAAATCCA CATGCCTGAG 
AAGACGGCGG TGGAGTTCCT GTCCAAGGCT GTGGACAGTC CAAACATCAA GGCAGTGGAC 
GAGGCTGTGA TCTTGCTCCA GGAGATCGGG GTGCTGGACC AGCGGGAGTA CCTGACTACC 
CTGGGGCAGC GCCTGGCTCA CATCTCCACC GACCCCCGGT TGGCCAAGGC CATTGTGTTG 
GCTGCCATCT TCCGTTGCCT GCACCCACTA CTGGTGGTCG TTTCCTGCCT CACCCGGGAC 
CCCTTCAGCA GCAGCCTACA GAACCGGGCA GAGGTGGACA AGGTGAAAGC ACTGTTGAGC 
CATGACAGCG GCAGTGACCA CCTGGCCTTT GTGCGGGCTG TCGCCGGCTG GGAGGAGGTG 
CTGCGTTGGC AGGACCGCAG CTCCCGGGAG AATTACCTGG AGGAAAACCT GCTGTACGCA 
CCCAGCCTGC GCTTCATCCA CGGACTCATC AAGCAGTTCT CAGAGAACAT TTATGAGGCC 
TTCCTGGTGG GGAAGCCCTC GGACTGCACC CTGGCCTCCG CCCAGTGCAA CGAGTACAGT 
GAGGAGGAGG AGCTGGTGAA GGGCGTGCTG ATGGCCGGCC TCTACCCCAA CCTCATCCAG 
GTGAGGCAGG GCAAGGTCAC CCGGCAGGGG AAGTTCAAGC CCAACAGCGT CACATATAGG 
ACCAAATCAG GCAACATCCT GCTGCACAAG TCGACCATTA ACAGGGAGGC CACACGGTTA 
CGGAGCCGAT GGCTGACGTA TTTCATGGCA GTCAAGTCCA ATGGCAGCGT CTTCGTCCGG 
GACTCCTCTC AGGTGCACCC GCTAGCTGTG CTGCTGCTGA CCGACGGGGA CGTGCACATC 
CGTGATGACG GGCGCCGGGC CACCATCTCA CTGAGCGACA GTGACCTGCT GCGGCTGGAG 
GGTGACTCGC GTACCGTGCG GCTGCTGAAG GAGCTGCGGC GGGCCCTGGG CCGCATGGTG 
GAGCGGAGCC TGCGCAGCGA GCTGGCTGCA CTTCCCCCCA GCGTACAGGA GGAGCACGGG 
CAGCTGCTTG CGCTACTGGC AGAGCTGCTG CGAGGACCCT GTGGCAGCTT TGATGTGCGC 
AAGACAGCTG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531794.1
CDS	488..4285
Translation	MPSPRLGLRVRPARRGRAALGPAAPVLFARSLPRTGLGVGGSTMAAARRLMALAAGISPRLQPLGPRAAGRQGRSRGFSSSCAHPDHTKEAAEAESGMAPGGPGEGDGSLVNASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_011533492.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533492.2

ORF Insert Sequence:

ATGCCCTCGC CTAGGCTTGG GCTTCGGGTC CGCCCGGCCC GCAGGGGGCG CGCGGCGCTC 
GGGCCGGCCG CTCCCGTTCT CTTCGCCCGG TCCCTGCCGC GCACAGGCCT CGGGGTCGGC 
GGGAGCACGA TGGCGGCCGC TAGGAGACTC ATGGCGCTGG CCGCCGGCAT CTCTCCGCGC 
CTGCAGCCGC TGGGTCCCCG CGCTGCTGGG CGACAGGGTC GCTCGCGCGG CTTCTCTTCA 
AGCTGCGCCC ACCCCGACCA CACCAAGGAA GCCGCCGAGG CCGAGTCAGG GATGGCCCCC 
GGCGGGCCTG GGGAAGGCGA CGGAAGCTTG GTGAACGCTT CTAGGGACCT ATTAAAAGAG 
TTCCCACAGC CCAAAAATCT TCTCAACAGT GTGATTGGAA GAGCCCTCGG CATCTCACAT 
GCAAAAGACA AACTAGTCTA CGTGCACACA AATGGACCGA AGAAAAAGAA AGTCACACTG 
CACATAAAAT GGCCCAAGAG CGTGGAGGTA GAAGGCTATG GCAGCAAGAA GATCGATGCT 
GAGCGGCAGG CTGCAGCTGC AGCCTGCCAG CTGTTCAAGG GTTGGGGTCT GCTAGGTCCC 
CGGAATGAGT TGTTTGACGC AGCCAAATAC CGAGTGCTAG CTGATCGCTT TGGCTCCCCT 
GCCGACAGCT GGTGGCGTCC GGAACCCACC ATGCCCCCTA CTTCCTGGCG GCAGCTGAAT 
CCAGAGAGTA TTCGACCAGG GGGACCTGGG GGCCTATCCC GCTCTTTAGG CCGGGAAGAA 
GAGGAGGACG AGGAGGAAGA GCTAGAAGAA GGGACCATAG ATGTTACCGA CTTCTTGTCC 
ATGACCCAGC AGGATTCCCA CGCTCCACTC AGGGACTCAA GGGGGAGTTC CTTTGAGATG 
ACAGATGACG ACAGTGCCAT TAGGGCTCTG ACCCAGTTTC CACTTCCCAA GAACCTTCTG 
GCCAAGGTGA TTCAGATTGC AACGTCATCC TCCACAGCTA AGAACCTCAT GCAGTTCCAT 
ACTGTGGGCA CCAAGACCAA GCTGTCTACA CTCACCCTGC TCTGGCCCTG CCCCATGACC 
TTTGTTGCCA AAGGGCGCCG CAAAGCAGAG GCTGAGAATA AGGCGGCAGC CTTGGCCTGC 
AAGAAACTGA AGAGCCTGGG CCTGGTGGAC AGGAACAACG AACCGCTTAC ACACGCCATG 
TATAACCTGG CCTCTTTGCG TGAGCTGGGT GAGACCCAGC GCCGACCATG CACCATCCAG 
GTGCCCGAGC CCATCCTCCG CAAGATAGAG ACCTTCCTGA ACCATTACCC TGTGGAGAGT 
TCATGGATCG CCCCAGAACT CCGGCTGCAG AGTGATGACA TCTTGCCCTT GGGCAAGGAC 
TCAGGGCCTC TGAGTGACCC TATCACAGGC AAGCCCTATG TGCCCCTGTT GGAAGCAGAG 
GAGGTACGTC TCAGCCAGAG TCTGCTAGAA CTGTGGCGGC GGCGAGGGCC GGTCTGGCAG 
GAGGCCCCCC AGCTACCTGT GGACCCACAT CGGGACACCA TCCTCAACGC CATTGAGCAG 
CACCCGGTGG TGGTCATCTC TGGGGACACG GGCTGTGGGA AGACCACGCG CATCCCCCAG 
CTGTTGCTGG AGCGCTATGT GACCGAGGGC CGAGGTGCCC GCTGCAATGT TATCATCACC 
CAACCTCGCC GCATCTCTGC TGTGTCTGTG GCACAGCGGG TCAGCCACGA ACTGGGCCCC 
TCCCTGCGCC GGAATGTGGG CTTCCAGGTG CGGTTGGAAA GTAAGCCCCC ATCCCGAGGC 
GGGGCCCTGC TCTTCTGCAC TGTGGGTATC CTGCTGCGTA AGCTGCAGAG CAACCCCAGC 
CTGGAGGGCG TGAGCCACGT CATCGTGGAT GAGGTGCATG AGCGGGACGT GAACACAGAC 
TTTCTGCTGA TCCTGCTCAA GGGCCTGCAG CGGCTCAACC CGGCCCTGCG GCTGGTGCTC 
ATGAGTGCCA CAGGGGACAA TGAGCGCTTC TCCCGATACT TTGGTGGCTG CCCCGTCATC 
AAGGTGCCTG GCTTCATGTA CCCAGTCAAG GAGCACTACC TAGAGGACAT CCTGGCCAAG 
TTGGGCAAGC ACCAGTACCT GCACCGGCAC CGGCACCATG AGTCTGAGGA TGAATGCGCA 
CTCGATTTGG ACCTTGTGAC TGATCTGGTT CTGCACATCG ATGCTCGCGG GGAACCAGGT 
GGGATCCTGT GCTTCCTGCC TGGGTGGCAG GAGATCAAAG GAGTGCAGCA GCGCCTCCAG 
GAGGCCCTGG GCATGCACGA GAGCAAGTAC CTCATCCTGC CAGTGCACTC CAACATCCCC 
ATGATGGATC AGAAGGCCAT ATTCCAGCAG CCTCCAGTTG GGGTGCGCAA GATTGTCTTG 
GCCACCAACA TTGCTGAGAC TTCCATCACA ATCAATGACA TCGTGCATGT GGTGGACAGT 
GGGCTGCACA AGGAAGAACG CTATGACCTG AAGACCAAGG TGTCCTGCCT GGAGACAGTG 
TGGGTATCAA GAGCCAATGT GATCCAGCGC CGGGGCCGGG CGGGCCGCTG CCAGTCCGGC 
TTTGCCTACC ACTTGTTCCC TCGAAGCCGG CTGGAGAAAA TGGTCCCTTT CCAAGTGCCA 
GAGATCCTGC GCACACCTCT TGAGAACCTG GTGCTGCAAG CGAAAATCCA CATGCCTGAG 
AAGACGGCGG TGGAGTTCCT GTCCAAGGCT GTGGACAGTC CAAACATCAA GGCAGTGGAC 
GAGGCTGTGA TCTTGCTCCA GGAGATCGGG GTGCTGGACC AGCGGGAGTA CCTGACTACC 
CTGGGGCAGC GCCTGGCTCA CATCTCCACC GACCCCCGGT TGGCCAAGGC CATTGTGTTG 
GCTGCCATCT TCCGTTGCCT GCACCCACTA CTGGTGGTCG TTTCCTGCCT CACCCGGGAC 
CCCTTCAGCA GCAGCCTACA GAACCGGGCA GAGGTGGACA AGGTGAAAGC ACTGTTGAGC 
CATGACAGCG GCAGTGACCA CCTGGCCTTT GTGCGGGCTG TCGCCGGCTG GGAGGAGGTG 
CTGCGTTGGC AGGACCGCAG CTCCCGGGAG AATTACCTGG AGGAAAACCT GCTGTACGCA 
CCCAGCCTGC GCTTCATCCA CGGACTCATC AAGCAGTTCT CAGAGAACAT TTATGAGGCC 
TTCCTGGTGG GGAAGCCCTC GGACTGCACC CTGGCCTCCG CCCAGTGCAA CGAGTACAGT 
GAGGAGGAGG AGCTGGTGAA GGGCGTGCTG ATGGCCGGCC TCTACCCCAA CCTCATCCAG 
GTGAGGCAGG GCAAGGTCAC CCGGCAGGGG AAGTTCAAGC CCAACAGCGT CACATATAGG 
ACCAAATCAG GCAACATCCT GCTGCACAAG TCGACCATTA ACAGGGAGGC CACACGGTTA 
CGGAGCCGAT GGCTGACGTA TTTCATGGCA GTCAAGTCCA ATGGCAGCGT CTTCGTCCGG 
GACTCCTCTC AGGTGCACCC GCTAGCTGTG CTGCTGCTGA CCGACGGGGA CGTGCACATC 
CGTGATGACG GGCGCCGGGC CACCATCTCA CTGAGCGACA GTGACCTGCT GCGGCTGGAG 
GGTGACTCGC GTACCGTGCG GCTGCTGAAG GAGCTGCGGC GGGCCCTGGG CCGCATGGTG 
GAGCGGAGCC TGCGCAGCGA GCTGGCTGCA CTTCCCCCCA GCGTACAGGA GGAGCACGGG 
CAGCTGCTTG CGCTACTGGC AGAGCTGCTG CGAGGACCCT GTGGCAGCTT TGATGTGCGC 
AAGACAGCTG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_011533491.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531793.1 Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu65125
Clone ID Related Accession (Same CDS sequence)	XM_011533490.1 , XM_011533490.2 , XM_011533492.2 , XM_011533491.1
Accession Version	XM_011533491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGC CTAGGCTTGG GCTTCGGGTC CGCCCGGCCC GCAGGGGGCG CGCGGCGCTC 
GGGCCGGCCG CTCCCGTTCT CTTCGCCCGG TCCCTGCCGC GCACAGGCCT CGGGGTCGGC 
GGGAGCACGA TGGCGGCCGC TAGGAGACTC ATGGCGCTGG CCGCCGGCAT CTCTCCGCGC 
CTGCAGCCGC TGGGTCCCCG CGCTGCTGGG CGACAGGGTC GCTCGCGCGG CTTCTCTTCA 
AGCTGCGCCC ACCCCGACCA CACCAAGGAA GCCGCCGAGG CCGAGTCAGG GATGGCCCCC 
GGCGGGCCTG GGGAAGGCGA CGGAAGCTTG GTGAACGCTT CTAGGGACCT ATTAAAAGAG 
TTCCCACAGC CCAAAAATCT TCTCAACAGT GTGATTGGAA GAGCCCTCGG CATCTCACAT 
GCAAAAGACA AACTAGTCTA CGTGCACACA AATGGACCGA AGAAAAAGAA AGTCACACTG 
CACATAAAAT GGCCCAAGAG CGTGGAGGTA GAAGGCTATG GCAGCAAGAA GATCGATGCT 
GAGCGGCAGG CTGCAGCTGC AGCCTGCCAG CTGTTCAAGG GTTGGGGTCT GCTAGGTCCC 
CGGAATGAGT TGTTTGACGC AGCCAAATAC CGAGTGCTAG CTGATCGCTT TGGCTCCCCT 
GCCGACAGCT GGTGGCGTCC GGAACCCACC ATGCCCCCTA CTTCCTGGCG GCAGCTGAAT 
CCAGAGAGTA TTCGACCAGG GGGACCTGGG GGCCTATCCC GCTCTTTAGG CCGGGAAGAA 
GAGGAGGACG AGGAGGAAGA GCTAGAAGAA GGGACCATAG ATGTTACCGA CTTCTTGTCC 
ATGACCCAGC AGGATTCCCA CGCTCCACTC AGGGACTCAA GGGGGAGTTC CTTTGAGATG 
ACAGATGACG ACAGTGCCAT TAGGGCTCTG ACCCAGTTTC CACTTCCCAA GAACCTTCTG 
GCCAAGGTGA TTCAGATTGC AACGTCATCC TCCACAGCTA AGAACCTCAT GCAGTTCCAT 
ACTGTGGGCA CCAAGACCAA GCTGTCTACA CTCACCCTGC TCTGGCCCTG CCCCATGACC 
TTTGTTGCCA AAGGGCGCCG CAAAGCAGAG GCTGAGAATA AGGCGGCAGC CTTGGCCTGC 
AAGAAACTGA AGAGCCTGGG CCTGGTGGAC AGGAACAACG AACCGCTTAC ACACGCCATG 
TATAACCTGG CCTCTTTGCG TGAGCTGGGT GAGACCCAGC GCCGACCATG CACCATCCAG 
GTGCCCGAGC CCATCCTCCG CAAGATAGAG ACCTTCCTGA ACCATTACCC TGTGGAGAGT 
TCATGGATCG CCCCAGAACT CCGGCTGCAG AGTGATGACA TCTTGCCCTT GGGCAAGGAC 
TCAGGGCCTC TGAGTGACCC TATCACAGGC AAGCCCTATG TGCCCCTGTT GGAAGCAGAG 
GAGGTACGTC TCAGCCAGAG TCTGCTAGAA CTGTGGCGGC GGCGAGGGCC GGTCTGGCAG 
GAGGCCCCCC AGCTACCTGT GGACCCACAT CGGGACACCA TCCTCAACGC CATTGAGCAG 
CACCCGGTGG TGGTCATCTC TGGGGACACG GGCTGTGGGA AGACCACGCG CATCCCCCAG 
CTGTTGCTGG AGCGCTATGT GACCGAGGGC CGAGGTGCCC GCTGCAATGT TATCATCACC 
CAACCTCGCC GCATCTCTGC TGTGTCTGTG GCACAGCGGG TCAGCCACGA ACTGGGCCCC 
TCCCTGCGCC GGAATGTGGG CTTCCAGGTG CGGTTGGAAA GTAAGCCCCC ATCCCGAGGC 
GGGGCCCTGC TCTTCTGCAC TGTGGGTATC CTGCTGCGTA AGCTGCAGAG CAACCCCAGC 
CTGGAGGGCG TGAGCCACGT CATCGTGGAT GAGGTGCATG AGCGGGACGT GAACACAGAC 
TTTCTGCTGA TCCTGCTCAA GGGCCTGCAG CGGCTCAACC CGGCCCTGCG GCTGGTGCTC 
ATGAGTGCCA CAGGGGACAA TGAGCGCTTC TCCCGATACT TTGGTGGCTG CCCCGTCATC 
AAGGTGCCTG GCTTCATGTA CCCAGTCAAG GAGCACTACC TAGAGGACAT CCTGGCCAAG 
TTGGGCAAGC ACCAGTACCT GCACCGGCAC CGGCACCATG AGTCTGAGGA TGAATGCGCA 
CTCGATTTGG ACCTTGTGAC TGATCTGGTT CTGCACATCG ATGCTCGCGG GGAACCAGGT 
GGGATCCTGT GCTTCCTGCC TGGGTGGCAG GAGATCAAAG GAGTGCAGCA GCGCCTCCAG 
GAGGCCCTGG GCATGCACGA GAGCAAGTAC CTCATCCTGC CAGTGCACTC CAACATCCCC 
ATGATGGATC AGAAGGCCAT ATTCCAGCAG CCTCCAGTTG GGGTGCGCAA GATTGTCTTG 
GCCACCAACA TTGCTGAGAC TTCCATCACA ATCAATGACA TCGTGCATGT GGTGGACAGT 
GGGCTGCACA AGGAAGAACG CTATGACCTG AAGACCAAGG TGTCCTGCCT GGAGACAGTG 
TGGGTATCAA GAGCCAATGT GATCCAGCGC CGGGGCCGGG CGGGCCGCTG CCAGTCCGGC 
TTTGCCTACC ACTTGTTCCC TCGAAGCCGG CTGGAGAAAA TGGTCCCTTT CCAAGTGCCA 
GAGATCCTGC GCACACCTCT TGAGAACCTG GTGCTGCAAG CGAAAATCCA CATGCCTGAG 
AAGACGGCGG TGGAGTTCCT GTCCAAGGCT GTGGACAGTC CAAACATCAA GGCAGTGGAC 
GAGGCTGTGA TCTTGCTCCA GGAGATCGGG GTGCTGGACC AGCGGGAGTA CCTGACTACC 
CTGGGGCAGC GCCTGGCTCA CATCTCCACC GACCCCCGGT TGGCCAAGGC CATTGTGTTG 
GCTGCCATCT TCCGTTGCCT GCACCCACTA CTGGTGGTCG TTTCCTGCCT CACCCGGGAC 
CCCTTCAGCA GCAGCCTACA GAACCGGGCA GAGGTGGACA AGGTGAAAGC ACTGTTGAGC 
CATGACAGCG GCAGTGACCA CCTGGCCTTT GTGCGGGCTG TCGCCGGCTG GGAGGAGGTG 
CTGCGTTGGC AGGACCGCAG CTCCCGGGAG AATTACCTGG AGGAAAACCT GCTGTACGCA 
CCCAGCCTGC GCTTCATCCA CGGACTCATC AAGCAGTTCT CAGAGAACAT TTATGAGGCC 
TTCCTGGTGG GGAAGCCCTC GGACTGCACC CTGGCCTCCG CCCAGTGCAA CGAGTACAGT 
GAGGAGGAGG AGCTGGTGAA GGGCGTGCTG ATGGCCGGCC TCTACCCCAA CCTCATCCAG 
GTGAGGCAGG GCAAGGTCAC CCGGCAGGGG AAGTTCAAGC CCAACAGCGT CACATATAGG 
ACCAAATCAG GCAACATCCT GCTGCACAAG TCGACCATTA ACAGGGAGGC CACACGGTTA 
CGGAGCCGAT GGCTGACGTA TTTCATGGCA GTCAAGTCCA ATGGCAGCGT CTTCGTCCGG 
GACTCCTCTC AGGTGCACCC GCTAGCTGTG CTGCTGCTGA CCGACGGGGA CGTGCACATC 
CGTGATGACG GGCGCCGGGC CACCATCTCA CTGAGCGACA GTGACCTGCT GCGGCTGGAG 
GGTGACTCGC GTACCGTGCG GCTGCTGAAG GAGCTGCGGC GGGCCCTGGG CCGCATGGTG 
GAGCGGAGCC TGCGCAGCGA GCTGGCTGCA CTTCCCCCCA GCGTACAGGA GGAGCACGGG 
CAGCTGCTTG CGCTACTGGC AGAGCTGCTG CGAGGACCCT GTGGCAGCTT TGATGTGCGC 
AAGACAGCTG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531793.1
CDS	656..4453
Translation	MPSPRLGLRVRPARRGRAALGPAAPVLFARSLPRTGLGVGGSTMAAARRLMALAAGISPRLQPLGPRAAGRQGRSRGFSSSCAHPDHTKEAAEAESGMAPGGPGEGDGSLVNASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_011533491.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533491.1

ORF Insert Sequence:

ATGCCCTCGC CTAGGCTTGG GCTTCGGGTC CGCCCGGCCC GCAGGGGGCG CGCGGCGCTC 
GGGCCGGCCG CTCCCGTTCT CTTCGCCCGG TCCCTGCCGC GCACAGGCCT CGGGGTCGGC 
GGGAGCACGA TGGCGGCCGC TAGGAGACTC ATGGCGCTGG CCGCCGGCAT CTCTCCGCGC 
CTGCAGCCGC TGGGTCCCCG CGCTGCTGGG CGACAGGGTC GCTCGCGCGG CTTCTCTTCA 
AGCTGCGCCC ACCCCGACCA CACCAAGGAA GCCGCCGAGG CCGAGTCAGG GATGGCCCCC 
GGCGGGCCTG GGGAAGGCGA CGGAAGCTTG GTGAACGCTT CTAGGGACCT ATTAAAAGAG 
TTCCCACAGC CCAAAAATCT TCTCAACAGT GTGATTGGAA GAGCCCTCGG CATCTCACAT 
GCAAAAGACA AACTAGTCTA CGTGCACACA AATGGACCGA AGAAAAAGAA AGTCACACTG 
CACATAAAAT GGCCCAAGAG CGTGGAGGTA GAAGGCTATG GCAGCAAGAA GATCGATGCT 
GAGCGGCAGG CTGCAGCTGC AGCCTGCCAG CTGTTCAAGG GTTGGGGTCT GCTAGGTCCC 
CGGAATGAGT TGTTTGACGC AGCCAAATAC CGAGTGCTAG CTGATCGCTT TGGCTCCCCT 
GCCGACAGCT GGTGGCGTCC GGAACCCACC ATGCCCCCTA CTTCCTGGCG GCAGCTGAAT 
CCAGAGAGTA TTCGACCAGG GGGACCTGGG GGCCTATCCC GCTCTTTAGG CCGGGAAGAA 
GAGGAGGACG AGGAGGAAGA GCTAGAAGAA GGGACCATAG ATGTTACCGA CTTCTTGTCC 
ATGACCCAGC AGGATTCCCA CGCTCCACTC AGGGACTCAA GGGGGAGTTC CTTTGAGATG 
ACAGATGACG ACAGTGCCAT TAGGGCTCTG ACCCAGTTTC CACTTCCCAA GAACCTTCTG 
GCCAAGGTGA TTCAGATTGC AACGTCATCC TCCACAGCTA AGAACCTCAT GCAGTTCCAT 
ACTGTGGGCA CCAAGACCAA GCTGTCTACA CTCACCCTGC TCTGGCCCTG CCCCATGACC 
TTTGTTGCCA AAGGGCGCCG CAAAGCAGAG GCTGAGAATA AGGCGGCAGC CTTGGCCTGC 
AAGAAACTGA AGAGCCTGGG CCTGGTGGAC AGGAACAACG AACCGCTTAC ACACGCCATG 
TATAACCTGG CCTCTTTGCG TGAGCTGGGT GAGACCCAGC GCCGACCATG CACCATCCAG 
GTGCCCGAGC CCATCCTCCG CAAGATAGAG ACCTTCCTGA ACCATTACCC TGTGGAGAGT 
TCATGGATCG CCCCAGAACT CCGGCTGCAG AGTGATGACA TCTTGCCCTT GGGCAAGGAC 
TCAGGGCCTC TGAGTGACCC TATCACAGGC AAGCCCTATG TGCCCCTGTT GGAAGCAGAG 
GAGGTACGTC TCAGCCAGAG TCTGCTAGAA CTGTGGCGGC GGCGAGGGCC GGTCTGGCAG 
GAGGCCCCCC AGCTACCTGT GGACCCACAT CGGGACACCA TCCTCAACGC CATTGAGCAG 
CACCCGGTGG TGGTCATCTC TGGGGACACG GGCTGTGGGA AGACCACGCG CATCCCCCAG 
CTGTTGCTGG AGCGCTATGT GACCGAGGGC CGAGGTGCCC GCTGCAATGT TATCATCACC 
CAACCTCGCC GCATCTCTGC TGTGTCTGTG GCACAGCGGG TCAGCCACGA ACTGGGCCCC 
TCCCTGCGCC GGAATGTGGG CTTCCAGGTG CGGTTGGAAA GTAAGCCCCC ATCCCGAGGC 
GGGGCCCTGC TCTTCTGCAC TGTGGGTATC CTGCTGCGTA AGCTGCAGAG CAACCCCAGC 
CTGGAGGGCG TGAGCCACGT CATCGTGGAT GAGGTGCATG AGCGGGACGT GAACACAGAC 
TTTCTGCTGA TCCTGCTCAA GGGCCTGCAG CGGCTCAACC CGGCCCTGCG GCTGGTGCTC 
ATGAGTGCCA CAGGGGACAA TGAGCGCTTC TCCCGATACT TTGGTGGCTG CCCCGTCATC 
AAGGTGCCTG GCTTCATGTA CCCAGTCAAG GAGCACTACC TAGAGGACAT CCTGGCCAAG 
TTGGGCAAGC ACCAGTACCT GCACCGGCAC CGGCACCATG AGTCTGAGGA TGAATGCGCA 
CTCGATTTGG ACCTTGTGAC TGATCTGGTT CTGCACATCG ATGCTCGCGG GGAACCAGGT 
GGGATCCTGT GCTTCCTGCC TGGGTGGCAG GAGATCAAAG GAGTGCAGCA GCGCCTCCAG 
GAGGCCCTGG GCATGCACGA GAGCAAGTAC CTCATCCTGC CAGTGCACTC CAACATCCCC 
ATGATGGATC AGAAGGCCAT ATTCCAGCAG CCTCCAGTTG GGGTGCGCAA GATTGTCTTG 
GCCACCAACA TTGCTGAGAC TTCCATCACA ATCAATGACA TCGTGCATGT GGTGGACAGT 
GGGCTGCACA AGGAAGAACG CTATGACCTG AAGACCAAGG TGTCCTGCCT GGAGACAGTG 
TGGGTATCAA GAGCCAATGT GATCCAGCGC CGGGGCCGGG CGGGCCGCTG CCAGTCCGGC 
TTTGCCTACC ACTTGTTCCC TCGAAGCCGG CTGGAGAAAA TGGTCCCTTT CCAAGTGCCA 
GAGATCCTGC GCACACCTCT TGAGAACCTG GTGCTGCAAG CGAAAATCCA CATGCCTGAG 
AAGACGGCGG TGGAGTTCCT GTCCAAGGCT GTGGACAGTC CAAACATCAA GGCAGTGGAC 
GAGGCTGTGA TCTTGCTCCA GGAGATCGGG GTGCTGGACC AGCGGGAGTA CCTGACTACC 
CTGGGGCAGC GCCTGGCTCA CATCTCCACC GACCCCCGGT TGGCCAAGGC CATTGTGTTG 
GCTGCCATCT TCCGTTGCCT GCACCCACTA CTGGTGGTCG TTTCCTGCCT CACCCGGGAC 
CCCTTCAGCA GCAGCCTACA GAACCGGGCA GAGGTGGACA AGGTGAAAGC ACTGTTGAGC 
CATGACAGCG GCAGTGACCA CCTGGCCTTT GTGCGGGCTG TCGCCGGCTG GGAGGAGGTG 
CTGCGTTGGC AGGACCGCAG CTCCCGGGAG AATTACCTGG AGGAAAACCT GCTGTACGCA 
CCCAGCCTGC GCTTCATCCA CGGACTCATC AAGCAGTTCT CAGAGAACAT TTATGAGGCC 
TTCCTGGTGG GGAAGCCCTC GGACTGCACC CTGGCCTCCG CCCAGTGCAA CGAGTACAGT 
GAGGAGGAGG AGCTGGTGAA GGGCGTGCTG ATGGCCGGCC TCTACCCCAA CCTCATCCAG 
GTGAGGCAGG GCAAGGTCAC CCGGCAGGGG AAGTTCAAGC CCAACAGCGT CACATATAGG 
ACCAAATCAG GCAACATCCT GCTGCACAAG TCGACCATTA ACAGGGAGGC CACACGGTTA 
CGGAGCCGAT GGCTGACGTA TTTCATGGCA GTCAAGTCCA ATGGCAGCGT CTTCGTCCGG 
GACTCCTCTC AGGTGCACCC GCTAGCTGTG CTGCTGCTGA CCGACGGGGA CGTGCACATC 
CGTGATGACG GGCGCCGGGC CACCATCTCA CTGAGCGACA GTGACCTGCT GCGGCTGGAG 
GGTGACTCGC GTACCGTGCG GCTGCTGAAG GAGCTGCGGC GGGCCCTGGG CCGCATGGTG 
GAGCGGAGCC TGCGCAGCGA GCTGGCTGCA CTTCCCCCCA GCGTACAGGA GGAGCACGGG 
CAGCTGCTTG CGCTACTGGC AGAGCTGCTG CGAGGACCCT GTGGCAGCTT TGATGTGCGC 
AAGACAGCTG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_011533493.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531795.1 Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X5, mRNA.

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CloneID	OHu65126
Clone ID Related Accession (Same CDS sequence)	XM_011533493.1 , XM_024453405.1
Accession Version	XM_011533493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative ATP-dependent RNA helicase DHX30 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGC CTAGGCTTGG GCTTCGGGTC CGCCCGGCCC GCAGGGGGCG CGCGGCGCTC 
GGGCCGGCCG CTCCCGTTCT CTTCGCCCGG TCCCTGCCGC GCACAGGCCT CGGGGTCGGC 
GGGAGCACGA TGGCGGCCGC TAGGAGACTC ATGGCGCTGG CCGCCGGCAT CTCTCCGCGC 
CTGCAGCCGC TGGGTCCCCG CGCTGCTGGG CGACAGGGTC GCTCGCGCGG CTTCTCTTCA 
AGCTGCGCCC ACCCCGACCA CACCAAGGAA GCCGCCGAGG CCGAGTCAGG GATGGCCCCC 
GGCGGGCCTG GGGAAGGCGA CGGAAGCTTG GTGAACGCTT CTAGGGACCT ATTAAAAGAG 
TTCCCACAGC CCAAAAATCT TCTCAACAGT GTGATTGGAA GAGCCCTCGG CATCTCACAT 
GCAAAAGACA AACTAGTCTA CGTGCACACA AATGGACCGA AGAAAAAGGG TTGGGGTCTG 
CTAGGTCCCC GGAATGAGTT GTTTGACGCA GCCAAATACC GAGTGCTAGC TGATCGCTTT 
GGCTCCCCTG CCGACAGCTG GTGGCGTCCG GAACCCACCA TGCCCCCTAC TTCCTGGCGG 
CAGCTGAATC CAGAGAGTAT TCGACCAGGG GGACCTGGGG GCCTATCCCG CTCTTTAGGC 
CGGGAAGAAG AGGAGGACGA GGAGGAAGAG CTAGAAGAAG GGACCATAGA TGTTACCGAC 
TTCTTGTCCA TGACCCAGCA GGATTCCCAC GCTCCACTCA GGGACTCAAG GGGGAGTTCC 
TTTGAGATGA CAGATGACGA CAGTGCCATT AGGGCTCTGA CCCAGTTTCC ACTTCCCAAG 
AACCTTCTGG CCAAGGTGAT TCAGATTGCA ACGTCATCCT CCACAGCTAA GAACCTCATG 
CAGTTCCATA CTGTGGGCAC CAAGACCAAG CTGTCTACAC TCACCCTGCT CTGGCCCTGC 
CCCATGACCT TTGTTGCCAA AGGGCGCCGC AAAGCAGAGG CTGAGAATAA GGCGGCAGCC 
TTGGCCTGCA AGAAACTGAA GAGCCTGGGC CTGGTGGACA GGAACAACGA ACCGCTTACA 
CACGCCATGT ATAACCTGGC CTCTTTGCGT GAGCTGGGTG AGACCCAGCG CCGACCATGC 
ACCATCCAGG TGCCCGAGCC CATCCTCCGC AAGATAGAGA CCTTCCTGAA CCATTACCCT 
GTGGAGAGTT CATGGATCGC CCCAGAACTC CGGCTGCAGA GTGATGACAT CTTGCCCTTG 
GGCAAGGACT CAGGGCCTCT GAGTGACCCT ATCACAGGCA AGCCCTATGT GCCCCTGTTG 
GAAGCAGAGG AGGTACGTCT CAGCCAGAGT CTGCTAGAAC TGTGGCGGCG GCGAGGGCCG 
GTCTGGCAGG AGGCCCCCCA GCTACCTGTG GACCCACATC GGGACACCAT CCTCAACGCC 
ATTGAGCAGC ACCCGGTGGT GGTCATCTCT GGGGACACGG GCTGTGGGAA GACCACGCGC 
ATCCCCCAGC TGTTGCTGGA GCGCTATGTG ACCGAGGGCC GAGGTGCCCG CTGCAATGTT 
ATCATCACCC AACCTCGCCG CATCTCTGCT GTGTCTGTGG CACAGCGGGT CAGCCACGAA 
CTGGGCCCCT CCCTGCGCCG GAATGTGGGC TTCCAGGTGC GGTTGGAAAG TAAGCCCCCA 
TCCCGAGGCG GGGCCCTGCT CTTCTGCACT GTGGGTATCC TGCTGCGTAA GCTGCAGAGC 
AACCCCAGCC TGGAGGGCGT GAGCCACGTC ATCGTGGATG AGGTGCATGA GCGGGACGTG 
AACACAGACT TTCTGCTGAT CCTGCTCAAG GGCCTGCAGC GGCTCAACCC GGCCCTGCGG 
CTGGTGCTCA TGAGTGCCAC AGGGGACAAT GAGCGCTTCT CCCGATACTT TGGTGGCTGC 
CCCGTCATCA AGGTGCCTGG CTTCATGTAC CCAGTCAAGG AGCACTACCT AGAGGACATC 
CTGGCCAAGT TGGGCAAGCA CCAGTACCTG CACCGGCACC GGCACCATGA GTCTGAGGAT 
GAATGCGCAC TCGATTTGGA CCTTGTGACT GATCTGGTTC TGCACATCGA TGCTCGCGGG 
GAACCAGGTG GGATCCTGTG CTTCCTGCCT GGGTGGCAGG AGATCAAAGG AGTGCAGCAG 
CGCCTCCAGG AGGCCCTGGG CATGCACGAG AGCAAGTACC TCATCCTGCC AGTGCACTCC 
AACATCCCCA TGATGGATCA GAAGGCCATA TTCCAGCAGC CTCCAGTTGG GGTGCGCAAG 
ATTGTCTTGG CCACCAACAT TGCTGAGACT TCCATCACAA TCAATGACAT CGTGCATGTG 
GTGGACAGTG GGCTGCACAA GGAAGAACGC TATGACCTGA AGACCAAGGT GTCCTGCCTG 
GAGACAGTGT GGGTATCAAG AGCCAATGTG ATCCAGCGCC GGGGCCGGGC GGGCCGCTGC 
CAGTCCGGCT TTGCCTACCA CTTGTTCCCT CGAAGCCGGC TGGAGAAAAT GGTCCCTTTC 
CAAGTGCCAG AGATCCTGCG CACACCTCTT GAGAACCTGG TGCTGCAAGC GAAAATCCAC 
ATGCCTGAGA AGACGGCGGT GGAGTTCCTG TCCAAGGCTG TGGACAGTCC AAACATCAAG 
GCAGTGGACG AGGCTGTGAT CTTGCTCCAG GAGATCGGGG TGCTGGACCA GCGGGAGTAC 
CTGACTACCC TGGGGCAGCG CCTGGCTCAC ATCTCCACCG ACCCCCGGTT GGCCAAGGCC 
ATTGTGTTGG CTGCCATCTT CCGTTGCCTG CACCCACTAC TGGTGGTCGT TTCCTGCCTC 
ACCCGGGACC CCTTCAGCAG CAGCCTACAG AACCGGGCAG AGGTGGACAA GGTGAAAGCA 
CTGTTGAGCC ATGACAGCGG CAGTGACCAC CTGGCCTTTG TGCGGGCTGT CGCCGGCTGG 
GAGGAGGTGC TGCGTTGGCA GGACCGCAGC TCCCGGGAGA ATTACCTGGA GGAAAACCTG 
CTGTACGCAC CCAGCCTGCG CTTCATCCAC GGACTCATCA AGCAGTTCTC AGAGAACATT 
TATGAGGCCT TCCTGGTGGG GAAGCCCTCG GACTGCACCC TGGCCTCCGC CCAGTGCAAC 
GAGTACAGTG AGGAGGAGGA GCTGGTGAAG GGCGTGCTGA TGGCCGGCCT CTACCCCAAC 
CTCATCCAGG TGAGGCAGGG CAAGGTCACC CGGCAGGGGA AGTTCAAGCC CAACAGCGTC 
ACATATAGGA CCAAATCAGG CAACATCCTG CTGCACAAGT CGACCATTAA CAGGGAGGCC 
ACACGGTTAC GGAGCCGATG GCTGACGTAT TTCATGGCAG TCAAGTCCAA TGGCAGCGTC 
TTCGTCCGGG ACTCCTCTCA GGTGCACCCG CTAGCTGTGC TGCTGCTGAC CGACGGGGAC 
GTGCACATCC GTGATGACGG GCGCCGGGCC ACCATCTCAC TGAGCGACAG TGACCTGCTG 
CGGCTGGAGG GTGACTCGCG TACCGTGCGG CTGCTGAAGG AGCTGCGGCG GGCCCTGGGC 
CGCATGGTGG AGCGGAGCCT GCGCAGCGAG CTGGCTGCAC TTCCCCCCAG CGTACAGGAG 
GAGCACGGGC AGCTGCTTGC GCTACTGGCA GAGCTGCTGC GAGGACCCTG TGGCAGCTTT 
GATGTGCGCA AGACAGCTGA CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531795.1
CDS	469..4155
Translation	MPSPRLGLRVRPARRGRAALGPAAPVLFARSLPRTGLGVGGSTMAAARRLMALAAGISPRLQPLGPRAAGRQGRSRGFSSSCAHPDHTKEAAEAESGMAPGGPGEGDGSLVNASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_011533493.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 30 (DHX30), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533493.1

ORF Insert Sequence:

ATGCCCTCGC CTAGGCTTGG GCTTCGGGTC CGCCCGGCCC GCAGGGGGCG CGCGGCGCTC 
GGGCCGGCCG CTCCCGTTCT CTTCGCCCGG TCCCTGCCGC GCACAGGCCT CGGGGTCGGC 
GGGAGCACGA TGGCGGCCGC TAGGAGACTC ATGGCGCTGG CCGCCGGCAT CTCTCCGCGC 
CTGCAGCCGC TGGGTCCCCG CGCTGCTGGG CGACAGGGTC GCTCGCGCGG CTTCTCTTCA 
AGCTGCGCCC ACCCCGACCA CACCAAGGAA GCCGCCGAGG CCGAGTCAGG GATGGCCCCC 
GGCGGGCCTG GGGAAGGCGA CGGAAGCTTG GTGAACGCTT CTAGGGACCT ATTAAAAGAG 
TTCCCACAGC CCAAAAATCT TCTCAACAGT GTGATTGGAA GAGCCCTCGG CATCTCACAT 
GCAAAAGACA AACTAGTCTA CGTGCACACA AATGGACCGA AGAAAAAGGG TTGGGGTCTG 
CTAGGTCCCC GGAATGAGTT GTTTGACGCA GCCAAATACC GAGTGCTAGC TGATCGCTTT 
GGCTCCCCTG CCGACAGCTG GTGGCGTCCG GAACCCACCA TGCCCCCTAC TTCCTGGCGG 
CAGCTGAATC CAGAGAGTAT TCGACCAGGG GGACCTGGGG GCCTATCCCG CTCTTTAGGC 
CGGGAAGAAG AGGAGGACGA GGAGGAAGAG CTAGAAGAAG GGACCATAGA TGTTACCGAC 
TTCTTGTCCA TGACCCAGCA GGATTCCCAC GCTCCACTCA GGGACTCAAG GGGGAGTTCC 
TTTGAGATGA CAGATGACGA CAGTGCCATT AGGGCTCTGA CCCAGTTTCC ACTTCCCAAG 
AACCTTCTGG CCAAGGTGAT TCAGATTGCA ACGTCATCCT CCACAGCTAA GAACCTCATG 
CAGTTCCATA CTGTGGGCAC CAAGACCAAG CTGTCTACAC TCACCCTGCT CTGGCCCTGC 
CCCATGACCT TTGTTGCCAA AGGGCGCCGC AAAGCAGAGG CTGAGAATAA GGCGGCAGCC 
TTGGCCTGCA AGAAACTGAA GAGCCTGGGC CTGGTGGACA GGAACAACGA ACCGCTTACA 
CACGCCATGT ATAACCTGGC CTCTTTGCGT GAGCTGGGTG AGACCCAGCG CCGACCATGC 
ACCATCCAGG TGCCCGAGCC CATCCTCCGC AAGATAGAGA CCTTCCTGAA CCATTACCCT 
GTGGAGAGTT CATGGATCGC CCCAGAACTC CGGCTGCAGA GTGATGACAT CTTGCCCTTG 
GGCAAGGACT CAGGGCCTCT GAGTGACCCT ATCACAGGCA AGCCCTATGT GCCCCTGTTG 
GAAGCAGAGG AGGTACGTCT CAGCCAGAGT CTGCTAGAAC TGTGGCGGCG GCGAGGGCCG 
GTCTGGCAGG AGGCCCCCCA GCTACCTGTG GACCCACATC GGGACACCAT CCTCAACGCC 
ATTGAGCAGC ACCCGGTGGT GGTCATCTCT GGGGACACGG GCTGTGGGAA GACCACGCGC 
ATCCCCCAGC TGTTGCTGGA GCGCTATGTG ACCGAGGGCC GAGGTGCCCG CTGCAATGTT 
ATCATCACCC AACCTCGCCG CATCTCTGCT GTGTCTGTGG CACAGCGGGT CAGCCACGAA 
CTGGGCCCCT CCCTGCGCCG GAATGTGGGC TTCCAGGTGC GGTTGGAAAG TAAGCCCCCA 
TCCCGAGGCG GGGCCCTGCT CTTCTGCACT GTGGGTATCC TGCTGCGTAA GCTGCAGAGC 
AACCCCAGCC TGGAGGGCGT GAGCCACGTC ATCGTGGATG AGGTGCATGA GCGGGACGTG 
AACACAGACT TTCTGCTGAT CCTGCTCAAG GGCCTGCAGC GGCTCAACCC GGCCCTGCGG 
CTGGTGCTCA TGAGTGCCAC AGGGGACAAT GAGCGCTTCT CCCGATACTT TGGTGGCTGC 
CCCGTCATCA AGGTGCCTGG CTTCATGTAC CCAGTCAAGG AGCACTACCT AGAGGACATC 
CTGGCCAAGT TGGGCAAGCA CCAGTACCTG CACCGGCACC GGCACCATGA GTCTGAGGAT 
GAATGCGCAC TCGATTTGGA CCTTGTGACT GATCTGGTTC TGCACATCGA TGCTCGCGGG 
GAACCAGGTG GGATCCTGTG CTTCCTGCCT GGGTGGCAGG AGATCAAAGG AGTGCAGCAG 
CGCCTCCAGG AGGCCCTGGG CATGCACGAG AGCAAGTACC TCATCCTGCC AGTGCACTCC 
AACATCCCCA TGATGGATCA GAAGGCCATA TTCCAGCAGC CTCCAGTTGG GGTGCGCAAG 
ATTGTCTTGG CCACCAACAT TGCTGAGACT TCCATCACAA TCAATGACAT CGTGCATGTG 
GTGGACAGTG GGCTGCACAA GGAAGAACGC TATGACCTGA AGACCAAGGT GTCCTGCCTG 
GAGACAGTGT GGGTATCAAG AGCCAATGTG ATCCAGCGCC GGGGCCGGGC GGGCCGCTGC 
CAGTCCGGCT TTGCCTACCA CTTGTTCCCT CGAAGCCGGC TGGAGAAAAT GGTCCCTTTC 
CAAGTGCCAG AGATCCTGCG CACACCTCTT GAGAACCTGG TGCTGCAAGC GAAAATCCAC 
ATGCCTGAGA AGACGGCGGT GGAGTTCCTG TCCAAGGCTG TGGACAGTCC AAACATCAAG 
GCAGTGGACG AGGCTGTGAT CTTGCTCCAG GAGATCGGGG TGCTGGACCA GCGGGAGTAC 
CTGACTACCC TGGGGCAGCG CCTGGCTCAC ATCTCCACCG ACCCCCGGTT GGCCAAGGCC 
ATTGTGTTGG CTGCCATCTT CCGTTGCCTG CACCCACTAC TGGTGGTCGT TTCCTGCCTC 
ACCCGGGACC CCTTCAGCAG CAGCCTACAG AACCGGGCAG AGGTGGACAA GGTGAAAGCA 
CTGTTGAGCC ATGACAGCGG CAGTGACCAC CTGGCCTTTG TGCGGGCTGT CGCCGGCTGG 
GAGGAGGTGC TGCGTTGGCA GGACCGCAGC TCCCGGGAGA ATTACCTGGA GGAAAACCTG 
CTGTACGCAC CCAGCCTGCG CTTCATCCAC GGACTCATCA AGCAGTTCTC AGAGAACATT 
TATGAGGCCT TCCTGGTGGG GAAGCCCTCG GACTGCACCC TGGCCTCCGC CCAGTGCAAC 
GAGTACAGTG AGGAGGAGGA GCTGGTGAAG GGCGTGCTGA TGGCCGGCCT CTACCCCAAC 
CTCATCCAGG TGAGGCAGGG CAAGGTCACC CGGCAGGGGA AGTTCAAGCC CAACAGCGTC 
ACATATAGGA CCAAATCAGG CAACATCCTG CTGCACAAGT CGACCATTAA CAGGGAGGCC 
ACACGGTTAC GGAGCCGATG GCTGACGTAT TTCATGGCAG TCAAGTCCAA TGGCAGCGTC 
TTCGTCCGGG ACTCCTCTCA GGTGCACCCG CTAGCTGTGC TGCTGCTGAC CGACGGGGAC 
GTGCACATCC GTGATGACGG GCGCCGGGCC ACCATCTCAC TGAGCGACAG TGACCTGCTG 
CGGCTGGAGG GTGACTCGCG TACCGTGCGG CTGCTGAAGG AGCTGCGGCG GGCCCTGGGC 
CGCATGGTGG AGCGGAGCCT GCGCAGCGAG CTGGCTGCAC TTCCCCCCAG CGTACAGGAG 
GAGCACGGGC AGCTGCTTGC GCTACTGGCA GAGCTGCTGC GAGGACCCTG TGGCAGCTTT 
GATGTGCGCA AGACAGCTGA CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_024453405.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309173.1 Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X3, mRNA.

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CloneID	OHu65126
Clone ID Related Accession (Same CDS sequence)	XM_011533493.1 , XM_024453405.1
Accession Version	XM_024453405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX30 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGC CTAGGCTTGG GCTTCGGGTC CGCCCGGCCC GCAGGGGGCG CGCGGCGCTC 
GGGCCGGCCG CTCCCGTTCT CTTCGCCCGG TCCCTGCCGC GCACAGGCCT CGGGGTCGGC 
GGGAGCACGA TGGCGGCCGC TAGGAGACTC ATGGCGCTGG CCGCCGGCAT CTCTCCGCGC 
CTGCAGCCGC TGGGTCCCCG CGCTGCTGGG CGACAGGGTC GCTCGCGCGG CTTCTCTTCA 
AGCTGCGCCC ACCCCGACCA CACCAAGGAA GCCGCCGAGG CCGAGTCAGG GATGGCCCCC 
GGCGGGCCTG GGGAAGGCGA CGGAAGCTTG GTGAACGCTT CTAGGGACCT ATTAAAAGAG 
TTCCCACAGC CCAAAAATCT TCTCAACAGT GTGATTGGAA GAGCCCTCGG CATCTCACAT 
GCAAAAGACA AACTAGTCTA CGTGCACACA AATGGACCGA AGAAAAAGGG TTGGGGTCTG 
CTAGGTCCCC GGAATGAGTT GTTTGACGCA GCCAAATACC GAGTGCTAGC TGATCGCTTT 
GGCTCCCCTG CCGACAGCTG GTGGCGTCCG GAACCCACCA TGCCCCCTAC TTCCTGGCGG 
CAGCTGAATC CAGAGAGTAT TCGACCAGGG GGACCTGGGG GCCTATCCCG CTCTTTAGGC 
CGGGAAGAAG AGGAGGACGA GGAGGAAGAG CTAGAAGAAG GGACCATAGA TGTTACCGAC 
TTCTTGTCCA TGACCCAGCA GGATTCCCAC GCTCCACTCA GGGACTCAAG GGGGAGTTCC 
TTTGAGATGA CAGATGACGA CAGTGCCATT AGGGCTCTGA CCCAGTTTCC ACTTCCCAAG 
AACCTTCTGG CCAAGGTGAT TCAGATTGCA ACGTCATCCT CCACAGCTAA GAACCTCATG 
CAGTTCCATA CTGTGGGCAC CAAGACCAAG CTGTCTACAC TCACCCTGCT CTGGCCCTGC 
CCCATGACCT TTGTTGCCAA AGGGCGCCGC AAAGCAGAGG CTGAGAATAA GGCGGCAGCC 
TTGGCCTGCA AGAAACTGAA GAGCCTGGGC CTGGTGGACA GGAACAACGA ACCGCTTACA 
CACGCCATGT ATAACCTGGC CTCTTTGCGT GAGCTGGGTG AGACCCAGCG CCGACCATGC 
ACCATCCAGG TGCCCGAGCC CATCCTCCGC AAGATAGAGA CCTTCCTGAA CCATTACCCT 
GTGGAGAGTT CATGGATCGC CCCAGAACTC CGGCTGCAGA GTGATGACAT CTTGCCCTTG 
GGCAAGGACT CAGGGCCTCT GAGTGACCCT ATCACAGGCA AGCCCTATGT GCCCCTGTTG 
GAAGCAGAGG AGGTACGTCT CAGCCAGAGT CTGCTAGAAC TGTGGCGGCG GCGAGGGCCG 
GTCTGGCAGG AGGCCCCCCA GCTACCTGTG GACCCACATC GGGACACCAT CCTCAACGCC 
ATTGAGCAGC ACCCGGTGGT GGTCATCTCT GGGGACACGG GCTGTGGGAA GACCACGCGC 
ATCCCCCAGC TGTTGCTGGA GCGCTATGTG ACCGAGGGCC GAGGTGCCCG CTGCAATGTT 
ATCATCACCC AACCTCGCCG CATCTCTGCT GTGTCTGTGG CACAGCGGGT CAGCCACGAA 
CTGGGCCCCT CCCTGCGCCG GAATGTGGGC TTCCAGGTGC GGTTGGAAAG TAAGCCCCCA 
TCCCGAGGCG GGGCCCTGCT CTTCTGCACT GTGGGTATCC TGCTGCGTAA GCTGCAGAGC 
AACCCCAGCC TGGAGGGCGT GAGCCACGTC ATCGTGGATG AGGTGCATGA GCGGGACGTG 
AACACAGACT TTCTGCTGAT CCTGCTCAAG GGCCTGCAGC GGCTCAACCC GGCCCTGCGG 
CTGGTGCTCA TGAGTGCCAC AGGGGACAAT GAGCGCTTCT CCCGATACTT TGGTGGCTGC 
CCCGTCATCA AGGTGCCTGG CTTCATGTAC CCAGTCAAGG AGCACTACCT AGAGGACATC 
CTGGCCAAGT TGGGCAAGCA CCAGTACCTG CACCGGCACC GGCACCATGA GTCTGAGGAT 
GAATGCGCAC TCGATTTGGA CCTTGTGACT GATCTGGTTC TGCACATCGA TGCTCGCGGG 
GAACCAGGTG GGATCCTGTG CTTCCTGCCT GGGTGGCAGG AGATCAAAGG AGTGCAGCAG 
CGCCTCCAGG AGGCCCTGGG CATGCACGAG AGCAAGTACC TCATCCTGCC AGTGCACTCC 
AACATCCCCA TGATGGATCA GAAGGCCATA TTCCAGCAGC CTCCAGTTGG GGTGCGCAAG 
ATTGTCTTGG CCACCAACAT TGCTGAGACT TCCATCACAA TCAATGACAT CGTGCATGTG 
GTGGACAGTG GGCTGCACAA GGAAGAACGC TATGACCTGA AGACCAAGGT GTCCTGCCTG 
GAGACAGTGT GGGTATCAAG AGCCAATGTG ATCCAGCGCC GGGGCCGGGC GGGCCGCTGC 
CAGTCCGGCT TTGCCTACCA CTTGTTCCCT CGAAGCCGGC TGGAGAAAAT GGTCCCTTTC 
CAAGTGCCAG AGATCCTGCG CACACCTCTT GAGAACCTGG TGCTGCAAGC GAAAATCCAC 
ATGCCTGAGA AGACGGCGGT GGAGTTCCTG TCCAAGGCTG TGGACAGTCC AAACATCAAG 
GCAGTGGACG AGGCTGTGAT CTTGCTCCAG GAGATCGGGG TGCTGGACCA GCGGGAGTAC 
CTGACTACCC TGGGGCAGCG CCTGGCTCAC ATCTCCACCG ACCCCCGGTT GGCCAAGGCC 
ATTGTGTTGG CTGCCATCTT CCGTTGCCTG CACCCACTAC TGGTGGTCGT TTCCTGCCTC 
ACCCGGGACC CCTTCAGCAG CAGCCTACAG AACCGGGCAG AGGTGGACAA GGTGAAAGCA 
CTGTTGAGCC ATGACAGCGG CAGTGACCAC CTGGCCTTTG TGCGGGCTGT CGCCGGCTGG 
GAGGAGGTGC TGCGTTGGCA GGACCGCAGC TCCCGGGAGA ATTACCTGGA GGAAAACCTG 
CTGTACGCAC CCAGCCTGCG CTTCATCCAC GGACTCATCA AGCAGTTCTC AGAGAACATT 
TATGAGGCCT TCCTGGTGGG GAAGCCCTCG GACTGCACCC TGGCCTCCGC CCAGTGCAAC 
GAGTACAGTG AGGAGGAGGA GCTGGTGAAG GGCGTGCTGA TGGCCGGCCT CTACCCCAAC 
CTCATCCAGG TGAGGCAGGG CAAGGTCACC CGGCAGGGGA AGTTCAAGCC CAACAGCGTC 
ACATATAGGA CCAAATCAGG CAACATCCTG CTGCACAAGT CGACCATTAA CAGGGAGGCC 
ACACGGTTAC GGAGCCGATG GCTGACGTAT TTCATGGCAG TCAAGTCCAA TGGCAGCGTC 
TTCGTCCGGG ACTCCTCTCA GGTGCACCCG CTAGCTGTGC TGCTGCTGAC CGACGGGGAC 
GTGCACATCC GTGATGACGG GCGCCGGGCC ACCATCTCAC TGAGCGACAG TGACCTGCTG 
CGGCTGGAGG GTGACTCGCG TACCGTGCGG CTGCTGAAGG AGCTGCGGCG GGCCCTGGGC 
CGCATGGTGG AGCGGAGCCT GCGCAGCGAG CTGGCTGCAC TTCCCCCCAG CGTACAGGAG 
GAGCACGGGC AGCTGCTTGC GCTACTGGCA GAGCTGCTGC GAGGACCCTG TGGCAGCTTT 
GATGTGCGCA AGACAGCTGA CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309173.1
CDS	219..3905
Translation	MPSPRLGLRVRPARRGRAALGPAAPVLFARSLPRTGLGVGGSTMAAARRLMALAAGISPRLQPLGPRAAGRQGRSRGFSSSCAHPDHTKEAAEAESGMAPGGPGEGDGSLVNASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_024453405.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DExH-box helicase 30 (DHX30), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453405.1

ORF Insert Sequence:

ATGCCCTCGC CTAGGCTTGG GCTTCGGGTC CGCCCGGCCC GCAGGGGGCG CGCGGCGCTC 
GGGCCGGCCG CTCCCGTTCT CTTCGCCCGG TCCCTGCCGC GCACAGGCCT CGGGGTCGGC 
GGGAGCACGA TGGCGGCCGC TAGGAGACTC ATGGCGCTGG CCGCCGGCAT CTCTCCGCGC 
CTGCAGCCGC TGGGTCCCCG CGCTGCTGGG CGACAGGGTC GCTCGCGCGG CTTCTCTTCA 
AGCTGCGCCC ACCCCGACCA CACCAAGGAA GCCGCCGAGG CCGAGTCAGG GATGGCCCCC 
GGCGGGCCTG GGGAAGGCGA CGGAAGCTTG GTGAACGCTT CTAGGGACCT ATTAAAAGAG 
TTCCCACAGC CCAAAAATCT TCTCAACAGT GTGATTGGAA GAGCCCTCGG CATCTCACAT 
GCAAAAGACA AACTAGTCTA CGTGCACACA AATGGACCGA AGAAAAAGGG TTGGGGTCTG 
CTAGGTCCCC GGAATGAGTT GTTTGACGCA GCCAAATACC GAGTGCTAGC TGATCGCTTT 
GGCTCCCCTG CCGACAGCTG GTGGCGTCCG GAACCCACCA TGCCCCCTAC TTCCTGGCGG 
CAGCTGAATC CAGAGAGTAT TCGACCAGGG GGACCTGGGG GCCTATCCCG CTCTTTAGGC 
CGGGAAGAAG AGGAGGACGA GGAGGAAGAG CTAGAAGAAG GGACCATAGA TGTTACCGAC 
TTCTTGTCCA TGACCCAGCA GGATTCCCAC GCTCCACTCA GGGACTCAAG GGGGAGTTCC 
TTTGAGATGA CAGATGACGA CAGTGCCATT AGGGCTCTGA CCCAGTTTCC ACTTCCCAAG 
AACCTTCTGG CCAAGGTGAT TCAGATTGCA ACGTCATCCT CCACAGCTAA GAACCTCATG 
CAGTTCCATA CTGTGGGCAC CAAGACCAAG CTGTCTACAC TCACCCTGCT CTGGCCCTGC 
CCCATGACCT TTGTTGCCAA AGGGCGCCGC AAAGCAGAGG CTGAGAATAA GGCGGCAGCC 
TTGGCCTGCA AGAAACTGAA GAGCCTGGGC CTGGTGGACA GGAACAACGA ACCGCTTACA 
CACGCCATGT ATAACCTGGC CTCTTTGCGT GAGCTGGGTG AGACCCAGCG CCGACCATGC 
ACCATCCAGG TGCCCGAGCC CATCCTCCGC AAGATAGAGA CCTTCCTGAA CCATTACCCT 
GTGGAGAGTT CATGGATCGC CCCAGAACTC CGGCTGCAGA GTGATGACAT CTTGCCCTTG 
GGCAAGGACT CAGGGCCTCT GAGTGACCCT ATCACAGGCA AGCCCTATGT GCCCCTGTTG 
GAAGCAGAGG AGGTACGTCT CAGCCAGAGT CTGCTAGAAC TGTGGCGGCG GCGAGGGCCG 
GTCTGGCAGG AGGCCCCCCA GCTACCTGTG GACCCACATC GGGACACCAT CCTCAACGCC 
ATTGAGCAGC ACCCGGTGGT GGTCATCTCT GGGGACACGG GCTGTGGGAA GACCACGCGC 
ATCCCCCAGC TGTTGCTGGA GCGCTATGTG ACCGAGGGCC GAGGTGCCCG CTGCAATGTT 
ATCATCACCC AACCTCGCCG CATCTCTGCT GTGTCTGTGG CACAGCGGGT CAGCCACGAA 
CTGGGCCCCT CCCTGCGCCG GAATGTGGGC TTCCAGGTGC GGTTGGAAAG TAAGCCCCCA 
TCCCGAGGCG GGGCCCTGCT CTTCTGCACT GTGGGTATCC TGCTGCGTAA GCTGCAGAGC 
AACCCCAGCC TGGAGGGCGT GAGCCACGTC ATCGTGGATG AGGTGCATGA GCGGGACGTG 
AACACAGACT TTCTGCTGAT CCTGCTCAAG GGCCTGCAGC GGCTCAACCC GGCCCTGCGG 
CTGGTGCTCA TGAGTGCCAC AGGGGACAAT GAGCGCTTCT CCCGATACTT TGGTGGCTGC 
CCCGTCATCA AGGTGCCTGG CTTCATGTAC CCAGTCAAGG AGCACTACCT AGAGGACATC 
CTGGCCAAGT TGGGCAAGCA CCAGTACCTG CACCGGCACC GGCACCATGA GTCTGAGGAT 
GAATGCGCAC TCGATTTGGA CCTTGTGACT GATCTGGTTC TGCACATCGA TGCTCGCGGG 
GAACCAGGTG GGATCCTGTG CTTCCTGCCT GGGTGGCAGG AGATCAAAGG AGTGCAGCAG 
CGCCTCCAGG AGGCCCTGGG CATGCACGAG AGCAAGTACC TCATCCTGCC AGTGCACTCC 
AACATCCCCA TGATGGATCA GAAGGCCATA TTCCAGCAGC CTCCAGTTGG GGTGCGCAAG 
ATTGTCTTGG CCACCAACAT TGCTGAGACT TCCATCACAA TCAATGACAT CGTGCATGTG 
GTGGACAGTG GGCTGCACAA GGAAGAACGC TATGACCTGA AGACCAAGGT GTCCTGCCTG 
GAGACAGTGT GGGTATCAAG AGCCAATGTG ATCCAGCGCC GGGGCCGGGC GGGCCGCTGC 
CAGTCCGGCT TTGCCTACCA CTTGTTCCCT CGAAGCCGGC TGGAGAAAAT GGTCCCTTTC 
CAAGTGCCAG AGATCCTGCG CACACCTCTT GAGAACCTGG TGCTGCAAGC GAAAATCCAC 
ATGCCTGAGA AGACGGCGGT GGAGTTCCTG TCCAAGGCTG TGGACAGTCC AAACATCAAG 
GCAGTGGACG AGGCTGTGAT CTTGCTCCAG GAGATCGGGG TGCTGGACCA GCGGGAGTAC 
CTGACTACCC TGGGGCAGCG CCTGGCTCAC ATCTCCACCG ACCCCCGGTT GGCCAAGGCC 
ATTGTGTTGG CTGCCATCTT CCGTTGCCTG CACCCACTAC TGGTGGTCGT TTCCTGCCTC 
ACCCGGGACC CCTTCAGCAG CAGCCTACAG AACCGGGCAG AGGTGGACAA GGTGAAAGCA 
CTGTTGAGCC ATGACAGCGG CAGTGACCAC CTGGCCTTTG TGCGGGCTGT CGCCGGCTGG 
GAGGAGGTGC TGCGTTGGCA GGACCGCAGC TCCCGGGAGA ATTACCTGGA GGAAAACCTG 
CTGTACGCAC CCAGCCTGCG CTTCATCCAC GGACTCATCA AGCAGTTCTC AGAGAACATT 
TATGAGGCCT TCCTGGTGGG GAAGCCCTCG GACTGCACCC TGGCCTCCGC CCAGTGCAAC 
GAGTACAGTG AGGAGGAGGA GCTGGTGAAG GGCGTGCTGA TGGCCGGCCT CTACCCCAAC 
CTCATCCAGG TGAGGCAGGG CAAGGTCACC CGGCAGGGGA AGTTCAAGCC CAACAGCGTC 
ACATATAGGA CCAAATCAGG CAACATCCTG CTGCACAAGT CGACCATTAA CAGGGAGGCC 
ACACGGTTAC GGAGCCGATG GCTGACGTAT TTCATGGCAG TCAAGTCCAA TGGCAGCGTC 
TTCGTCCGGG ACTCCTCTCA GGTGCACCCG CTAGCTGTGC TGCTGCTGAC CGACGGGGAC 
GTGCACATCC GTGATGACGG GCGCCGGGCC ACCATCTCAC TGAGCGACAG TGACCTGCTG 
CGGCTGGAGG GTGACTCGCG TACCGTGCGG CTGCTGAAGG AGCTGCGGCG GGCCCTGGGC 
CGCATGGTGG AGCGGAGCCT GCGCAGCGAG CTGGCTGCAC TTCCCCCCAG CGTACAGGAG 
GAGCACGGGC AGCTGCTTGC GCTACTGGCA GAGCTGCTGC GAGGACCCTG TGGCAGCTTT 
GATGTGCGCA AGACAGCTGA CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

De Novo Missense Mutations in DHX30 Impair Global Translation and Cause a Neurodevelopmental Disorder.
American journal of human genetics101(5)716-724(2017 Nov)
Lessel D,Schob C,K?ry S,Reijnders MRF,Harel T,Eldomery MK,Coban-Akdemir Z,Denecke J,Edvardson S,Colin E,Stegmann APA,Gerkes EH,Tessarech M,Bonneau D,Barth M,Besnard T,Cogn? B,Revah-Politi A,Strom TM,Rosenfeld JA,Yang Y,Posey JE,Immken L,Oundjian N,Helbig KL,Meeks N,Zegar K,Morton J,,Schieving JH,Claasen A,Huentelman M,Narayanan V,Ramsey K,,Brunner HG,Elpeleg O,Mercier S,B?zieau S,Kubisch C,Kleefstra T,Kindler S,Lupski JR,Kreienkamp HJ

Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.
Cell reports(2015 Feb)
Antonicka H,Shoubridge EA

In pursuit of synthetic modulators for the orphan retina-specific nuclear receptor NR2E3.
Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics29(3)298-309(2013 Apr)
Qin Q,Knapinska A,Dobri N,Madoux F,Chase P,Hodder P,Petrukhin K

Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS.
Nature methods10(4)307-14(2013 Apr)
Varjosalo M,Sacco R,Stukalov A,van Drogen A,Planyavsky M,Hauri S,Aebersold R,Bennett KL,Colinge J,Gstaiger M,Superti-Furga G

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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DHX30 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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