Dhx30 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dhx30
Entrez Gene ID	72831
Full Name	DEAH (Asp-Glu-Ala-His) box polypeptide 30
Synonyms	2810477H02Rik,C130058C04Rik,Ddx30,HELG,Ret-CoR
General protein information	Preferred Names DEAH (Asp-Glu-Ala-His) box polypeptide 30 Names putative ATP-dependent RNA helicase DHX30 DEAH box protein 30
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 F2

mRNA and Protein(s)

mRNA	Protein	Name
XM_017313613.1	XP_017169102.1	putative ATP-dependent RNA helicase DHX30 isoform X1
XM_006512301.3	XP_006512364.1	putative ATP-dependent RNA helicase DHX30 isoform X1
XM_006512301.4	XP_006512364.1	ATP-dependent RNA helicase DHX30 isoform X1
XM_006512303.2	XP_006512366.1	ATP-dependent RNA helicase DHX30 isoform X2
XM_011243029.2	XP_011241331.1	putative ATP-dependent RNA helicase DHX30 isoform X1
XM_006512300.1	XP_006512363.1	putative ATP-dependent RNA helicase DHX30 isoform X1
XM_006512303.1	XP_006512366.1	putative ATP-dependent RNA helicase DHX30 isoform X2
XM_006512310.3	XP_006512373.1	putative ATP-dependent RNA helicase DHX30 isoform X3
XM_030244640.1	XP_030100500.1	ATP-dependent RNA helicase DHX30 isoform X2
XM_030244641.1	XP_030100501.1	ATP-dependent RNA helicase DHX30 isoform X2
XM_006512304.1	XP_006512367.1	ATP-dependent RNA helicase DHX30 isoform X2
XM_030244646.1	XP_030100506.1	ATP-dependent RNA helicase DHX30 isoform X3
XM_011243029.3	XP_011241331.1	ATP-dependent RNA helicase DHX30 isoform X1
XM_006512300.2	XP_006512363.1	ATP-dependent RNA helicase DHX30 isoform X1
XM_017313614.1	XP_017169103.1	ATP-dependent RNA helicase DHX30 isoform X1
XM_030244645.1	XP_030100505.1	ATP-dependent RNA helicase DHX30 isoform X3
XM_006512305.3	XP_006512368.1	putative ATP-dependent RNA helicase DHX30 isoform X2
XM_006512299.2	XP_006512362.1	ATP-dependent RNA helicase DHX30 isoform X1
XM_006512298.4	XP_006512361.1	ATP-dependent RNA helicase DHX30 isoform X1
XM_017313615.1	XP_017169104.1	ATP-dependent RNA helicase DHX30 isoform X3
XM_006512310.4	XP_006512373.1	ATP-dependent RNA helicase DHX30 isoform X3
XM_017313616.1	XP_017169105.1	ATP-dependent RNA helicase DHX30 isoform X3
NM_001252682.1	NP_001239611.1	ATP-dependent RNA helicase DHX30 isoform 1
NM_133347.2	NP_579925.1	ATP-dependent RNA helicase DHX30 isoform 2
NM_001252683.1	NP_001239612.1	ATP-dependent RNA helicase DHX30 isoform 3
XM_006512299.1	XP_006512362.1	putative ATP-dependent RNA helicase DHX30 isoform X1
XM_006512298.3	XP_006512361.1	putative ATP-dependent RNA helicase DHX30 isoform X1
XM_006512305.4	XP_006512368.1	ATP-dependent RNA helicase DHX30 isoform X2
XM_030244642.1	XP_030100502.1	ATP-dependent RNA helicase DHX30 isoform X3
XM_030244643.1	XP_030100503.1	ATP-dependent RNA helicase DHX30 isoform X3
XM_030244644.1	XP_030100504.1	ATP-dependent RNA helicase DHX30 isoform X3
XM_030244647.1	XP_030100507.1	ATP-dependent RNA helicase DHX30 isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DHX30	NP_001070003.1
Xenopus tropicalis (tropical clawed frog)	dhx30	NP_001107694.1
Homo sapiens (human)	DHX30	NP_619520.1
Canis lupus familiaris (dog)	DHX30	XP_005632658.1
Rattus norvegicus (Norway rat)	Dhx30	NP_001013267.1
Gallus gallus (chicken)	DHX30	NP_001012869.1
	LOC711178	XP_001113231.2
Mus musculus (house mouse)	Dhx30	NP_001239612.1
Pan troglodytes (chimpanzee)	DHX30	XP_001155300.2
Danio rerio (zebrafish)	dhx30	XP_005171140.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The novel helicase helG (DHX30) is expressed during gastrulation in mice and has a structure similar to a human DExH box helicase.
Zheng HJ, Tsukahara M, Liu E, Ye L, Xiong H, Noguchi S, Suzuki K, Ji ZS
Stem cells and development24(3)372-83(2015 Feb)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Raftlin is involved in the nucleocapture complex to induce poly(I:C)-mediated TLR3 activation.
Watanabe A, Tatematsu M, Saeki K, Shibata S, Shime H, Yoshimura A, Obuse C, Seya T, Matsumoto M
The Journal of biological chemistry286(12)10702-11(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

An improved gene trap method "trapped" a helG gene expressed in mouse ES cells. The helG product shared a high level of homology with the DExH RNA helicase family. It is required for differentiation during embryogenesis.
Title: The novel helicase helG (DHX30) is expressed during gastrulation in mice and has a structure similar to a human DExH box helicase.

The following Dhx30 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dhx30 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13449	NM_001252683.1 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
OMu12947	XM_006512303.2 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu12947	XM_030244640.1 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu12947	XM_030244641.1 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu12947	XM_006512305.4 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu13452	XM_006512299.2 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu13452	XM_006512298.4 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu13452	XM_006512300.2 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu13452	XM_006512301.4 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu13452	XM_011243029.3 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu43563	XM_030244646.1 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu43563	XM_030244645.1 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu43563	XM_030244647.1 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu43563	XM_030244642.1 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu43563	XM_006512310.4 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu43563	XM_030244643.1 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu43563	XM_030244644.1 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu12947	NM_001252682.1 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu13452	NM_133347.2 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu12947	XM_006512304.1 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu13452	XM_017313613.1 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu13452	XM_017313614.1 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu43563	XM_017313616.1 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu43563	XM_017313615.1 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu13452	XM_006512301.3	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu12947	XM_006512303.1	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu13452	XM_011243029.2	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu13452	XM_006512300.1	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu43563	XM_006512310.3	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu13452	XM_006512299.1	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu13452	XM_006512298.3	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu12947	XM_006512305.3	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dhx30 ( NM_001252683.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239612.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu13449
Clone ID Related Accession (Same CDS sequence)	NM_001252683.1
Accession Version	NM_001252683.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ074324.1, BC016202.1 and BY540912.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) is longer and has a distinct N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.179701.1, BC016202.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCTG CCAGGCGGCT CATGGCGCTG GCTGCCGGCG TCTCTCCGCG CCTCCGGCCG 
CCGGATCCCC TTGTCGCTTC CGGGCGACAA GGATGTTCGC GCGGCTTCTC ATCAAGCTTT 
GTCCGCTCTG ATGGCACTCA GGAAGCTGCC GAGGTCGAAT CAGAGGTGGC CCCTAGCGAG 
CCTGGGGAGG GTGACGGAAG CATGGTGAAC GCTTCTAGGG ACCTATTAAA AGAGTTTCCG 
CAGCCTAAAA ACCTTCTCAA CAGCGTGATT GGAAGAGCCC TTGGCATCTC ACATGCAAAA 
GACAAGTTAG TCTATGTGCA CACGAATGGA CCGAAGAAAA AGAAAGTCAC CCTGCACATA 
AAGTGGCCCA AGAGCGTGGA GGTGGAAGGC TATGGCAGCA AGAAGATTGA TGCTGAGCGT 
CAGGCTGCAG CAGCTGCCTG CCAACTCTTC AAGGGCTGGG GTCTGCTGGG ACCACGGAAT 
GAGCTGTTTG ATGCAGCTAA ATACCGAGTG CTAGCTGATC GTTTTGGGTC TCCAGCTGAC 
AGCTGGTGGC GCCCAGAACC CACCATGCCT CCAACTTCCT GGCGGCAGCT GAATCCTGAG 
AACATTCGGC CAGGGGGTCC TGCAGGACTA TCCCGATCCT TAGGCCGAGA GGAAGAGGAA 
GATGAGGAGG AAGAGCTAGA AGAGGGGACC ATTGATGTGA CAGAGTTTCT GTCTATGACC 
CAGCAAGACT CCCACAACCC ACTCAGGGAC TCAAGGGGGG GCTCCTTTGA AATGACAGAT 
GATGACAGTG CTATCAGAGC TCTGACCCAG TTTCCACTTC CCAAGAACCT CCTGGCCAAA 
GTGATTCAGA TTGCAACCTC CTCCTCCACA GCTAAGAATC TCATGCAGTT CCATACTGTG 
GGTACCAAGA CCAAGCTGGC TACACTCACT CTGCTCTGGC CCTGTCCCAT GACCTTTGTT 
GCCAAAGGGC GACGCAAAGC TGAGGCTGAG AATAAGGCAG CAGCCTTGGC TTGCAAGAAA 
CTGAAGAGCC TTGGCCTTGT GGACAGGAAC AATGAGCCGC TTACCCATGC CATGTACAAC 
CTGGCCTCCC TGCGTGAGTT GGGTGAGACC CAGCGCCGGC CATGTACCAT CCAGGTGCCT 
GAGCCCATCC TTCGCAAGAT AGAGGCCTTC CTGAGTCATT ACCCGGTGGA CAGCTCATGG 
ATTTCCCCAG AACTCCGACT GCAGAGTGAT GACATCTTGC CCTTAGGCAA GGACTCAGGG 
CCCTTGAGTG ACCCTATCAC AGGCAAGCCA TACATGCCCC TGTCAGAAGC AGAGGAGGTG 
CGCTTAAGCC AGAGCCTGCT AGAGCTGTGG CGGAGGAGAG GGCCGATCTG GCAGGAGGCC 
CCGCAGCTAC CTGTAGACCC TCATCGGGAC ACTATCCTCA GTGCCATTGA ACAACACCCA 
GTGGTAGTCA TCTCTGGGGA CACAGGCTGT GGGAAGACCA CACGTATCCC TCAGCTGCTA 
TTGGAGCGCT ATGTGACTGA GGGTCGAGGT GCCCGCTGCA ATGTGATCAT CACACAACCT 
CGCCGGATCT CAGCTGTGTC TGTGGCACAG CGGGTCAGCC ATGAACTGGG CCCCTCCTTG 
CGCCGGAATG TGGGCTTCCA GGTACGCTTG GAAAGTAAGC CCCCAGCCCG AGGTGGGGCG 
CTGCTCTTCT GCACTGTGGG TATCCTGCTT CGGAAGCTGC AGAGCAACCC CAGCCTGGAG 
GGTGTGAGCC ATGTCATTGT GGATGAGGTC CATGAGCGGG ATGTGAACAC AGACTTCCTG 
CTGATTCTGC TCAAGGGCCT GCAGCGGCTC AACCCAGCTC TTCGGCTGGT GCTCATGAGT 
GCTACAGGAG ATAATGAGCG CTTTTCCCGA TACTTTGGGG GCTGCCCTGT TATCAAGGTA 
CCTGGCTTCA TGTATCCCGT CAAGGAACAC TACCTGGAGG ACATCTTGGC CAAGCTGGGT 
AAACATCAGT ACCCACACCG GCATCGGCAC CATGAGTCGG AAGATGAATG TGCACTTGAT 
TTGGACCTTG TGACTGACCT GGTTCTGCAT ATCGATGCCC GTGGGGAACC AGGTGGGATC 
CTGTGCTTTC TACCTGGCTG GCAAGAAATC AAAGGAGTGC AACAACGGCT CCAGGAGGCC 
CTGGGCATGC ATGAAAGCAA GTACCTCATC TTACCAGTGC ACTCCAATAT CCCCATGATG 
GACCAGAAGG CCATATTCCA ACAGCCTCCA CTTGGGGTAC GCAAGATTGT ATTGGCCACC 
AACATTGCTG AAACCTCCAT CACAGTTAAC GACATTGTAC ATGTCGTGGA CAGCGGTCTG 
CACAAGGAGG AACGCTATGA CCTGAAGACC AAGGTGTCCT GCCTGGAGAC TGTGTGGGTG 
TCGAGAGCAA ATGTCATTCA GCGCCGGGGC AGGGCAGGCC GCTGCCAGTC AGGTTTTGCC 
TACCACTTGT TCCCGAGGAG CCGGCTGGAG AAAATGGTTC CTTTCCAAGT GCCAGAGATC 
CTGCGCACAC CTCTTGAGAA CCTGGTGCTG CAAGCCAAAA TCCATATGCC TGAGAAGACG 
GCAGTGGAGT TCCTCTCTAA GGCTGTGGAC AGTCCAAATA TCAAGGCAGT GGATGAGGCC 
GTGATCCTGC TCCAGGAGAT TGGGGTGCTG GACCAGCGGG AGTATCTGAC CACCTTGGGA 
CAGCGCCTGG CCCACATCTC TACTGACCCC CGACTGGCCA AGGCCATAGT GTTGGCTGCC 
ATCTTCCGTT GCCTGCACCC ACTGCTGGTG GTTGTTTCCT GCCTTACCCG GGACCCCTTC 
AGCAGCAGTT TGCAGAACCG GGCAGAAGTA GACAAGGTGA AGGCATTGCT GAGCCATGAC 
AGTGGCAGTG ACCATTTGGC CTTCGTGCGG GCTGTGGCTG GCTGGGAGGA GGTACTGCGC 
TGGCAGGACC GTACCTCCAG GGAAAACTAC CTGGAAGAAA ACCTTCTGTA TGCCCCCAGC 
TTGCGCTTCA TCCACGGGCT CATCAAGCAG TTCTCAGAGA ACATTTATGA GGCTTTCCTA 
GTGGGGAAGC CCTCTGACTG CACACTGCCC TCTGCTCAGT GCAATGAGTA CAGCGAGGAA 
GAGGAGCTGG TGAAGGGTGT GCTGATGGCT GGCCTCTACC CCAACCTCAT CCAGGTGAGA 
CAAGGTAAGG TTACTCGGCA AGGCAAGTTC AAACCCAACA GTGTCACTTA CAGGACCAAA 
TCTGGCAACA TCTTGCTGCA TAAGTCAACC ATTAACAGGG AGGCTACCCG GTTACGGAGC 
CGATGGCTGA CATATTTCAT GGCCGTCAAG TCCAATGGTA GCGTCTTTGT TCGAGATTCC 
TCCCAGGTGC ACCCACTAGC TGTGTTGCTC CTAACAGATG GGGACGTGCA CATCCGAGAT 
GATGGGCGTC GGGCCACCAT CTCATTGAGT GACAGCGACC TGCTTCGGCT GGAAGGTGAT 
TCACGAACTG TGCGGTTGCT AAGGGAGTTT CGTCGAGCCC TAGGACGGAT GGTGGAGCGG 
AGCCTCCGCA GCGAGTTAGC TGCACTTCCT CTTAGTGTGC AGCAAGAACA CGGGCAGCTG 
CTTGCACTGC TGGCAGAGTT GCTTCGAGGA CCTTGTGGCA GCTTTGATAT GCGCAAGACA 
GCTGATGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239612.1
CDS	62..3733
Translation	MAAARRLMALAAGVSPRLRPPDPLVASGRQGCSRGFSSSFVRSDGTQEAAEVESEVAPSEPGEGDGSMVNASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	NM_001252683.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252683.1

ORF Insert Sequence:

ATGGCGGCTG CCAGGCGGCT CATGGCGCTG GCTGCCGGCG TCTCTCCGCG CCTCCGGCCG 
CCGGATCCCC TTGTCGCTTC CGGGCGACAA GGATGTTCGC GCGGCTTCTC ATCAAGCTTT 
GTCCGCTCTG ATGGCACTCA GGAAGCTGCC GAGGTCGAAT CAGAGGTGGC CCCTAGCGAG 
CCTGGGGAGG GTGACGGAAG CATGGTGAAC GCTTCTAGGG ACCTATTAAA AGAGTTTCCG 
CAGCCTAAAA ACCTTCTCAA CAGCGTGATT GGAAGAGCCC TTGGCATCTC ACATGCAAAA 
GACAAGTTAG TCTATGTGCA CACGAATGGA CCGAAGAAAA AGAAAGTCAC CCTGCACATA 
AAGTGGCCCA AGAGCGTGGA GGTGGAAGGC TATGGCAGCA AGAAGATTGA TGCTGAGCGT 
CAGGCTGCAG CAGCTGCCTG CCAACTCTTC AAGGGCTGGG GTCTGCTGGG ACCACGGAAT 
GAGCTGTTTG ATGCAGCTAA ATACCGAGTG CTAGCTGATC GTTTTGGGTC TCCAGCTGAC 
AGCTGGTGGC GCCCAGAACC CACCATGCCT CCAACTTCCT GGCGGCAGCT GAATCCTGAG 
AACATTCGGC CAGGGGGTCC TGCAGGACTA TCCCGATCCT TAGGCCGAGA GGAAGAGGAA 
GATGAGGAGG AAGAGCTAGA AGAGGGGACC ATTGATGTGA CAGAGTTTCT GTCTATGACC 
CAGCAAGACT CCCACAACCC ACTCAGGGAC TCAAGGGGGG GCTCCTTTGA AATGACAGAT 
GATGACAGTG CTATCAGAGC TCTGACCCAG TTTCCACTTC CCAAGAACCT CCTGGCCAAA 
GTGATTCAGA TTGCAACCTC CTCCTCCACA GCTAAGAATC TCATGCAGTT CCATACTGTG 
GGTACCAAGA CCAAGCTGGC TACACTCACT CTGCTCTGGC CCTGTCCCAT GACCTTTGTT 
GCCAAAGGGC GACGCAAAGC TGAGGCTGAG AATAAGGCAG CAGCCTTGGC TTGCAAGAAA 
CTGAAGAGCC TTGGCCTTGT GGACAGGAAC AATGAGCCGC TTACCCATGC CATGTACAAC 
CTGGCCTCCC TGCGTGAGTT GGGTGAGACC CAGCGCCGGC CATGTACCAT CCAGGTGCCT 
GAGCCCATCC TTCGCAAGAT AGAGGCCTTC CTGAGTCATT ACCCGGTGGA CAGCTCATGG 
ATTTCCCCAG AACTCCGACT GCAGAGTGAT GACATCTTGC CCTTAGGCAA GGACTCAGGG 
CCCTTGAGTG ACCCTATCAC AGGCAAGCCA TACATGCCCC TGTCAGAAGC AGAGGAGGTG 
CGCTTAAGCC AGAGCCTGCT AGAGCTGTGG CGGAGGAGAG GGCCGATCTG GCAGGAGGCC 
CCGCAGCTAC CTGTAGACCC TCATCGGGAC ACTATCCTCA GTGCCATTGA ACAACACCCA 
GTGGTAGTCA TCTCTGGGGA CACAGGCTGT GGGAAGACCA CACGTATCCC TCAGCTGCTA 
TTGGAGCGCT ATGTGACTGA GGGTCGAGGT GCCCGCTGCA ATGTGATCAT CACACAACCT 
CGCCGGATCT CAGCTGTGTC TGTGGCACAG CGGGTCAGCC ATGAACTGGG CCCCTCCTTG 
CGCCGGAATG TGGGCTTCCA GGTACGCTTG GAAAGTAAGC CCCCAGCCCG AGGTGGGGCG 
CTGCTCTTCT GCACTGTGGG TATCCTGCTT CGGAAGCTGC AGAGCAACCC CAGCCTGGAG 
GGTGTGAGCC ATGTCATTGT GGATGAGGTC CATGAGCGGG ATGTGAACAC AGACTTCCTG 
CTGATTCTGC TCAAGGGCCT GCAGCGGCTC AACCCAGCTC TTCGGCTGGT GCTCATGAGT 
GCTACAGGAG ATAATGAGCG CTTTTCCCGA TACTTTGGGG GCTGCCCTGT TATCAAGGTA 
CCTGGCTTCA TGTATCCCGT CAAGGAACAC TACCTGGAGG ACATCTTGGC CAAGCTGGGT 
AAACATCAGT ACCCACACCG GCATCGGCAC CATGAGTCGG AAGATGAATG TGCACTTGAT 
TTGGACCTTG TGACTGACCT GGTTCTGCAT ATCGATGCCC GTGGGGAACC AGGTGGGATC 
CTGTGCTTTC TACCTGGCTG GCAAGAAATC AAAGGAGTGC AACAACGGCT CCAGGAGGCC 
CTGGGCATGC ATGAAAGCAA GTACCTCATC TTACCAGTGC ACTCCAATAT CCCCATGATG 
GACCAGAAGG CCATATTCCA ACAGCCTCCA CTTGGGGTAC GCAAGATTGT ATTGGCCACC 
AACATTGCTG AAACCTCCAT CACAGTTAAC GACATTGTAC ATGTCGTGGA CAGCGGTCTG 
CACAAGGAGG AACGCTATGA CCTGAAGACC AAGGTGTCCT GCCTGGAGAC TGTGTGGGTG 
TCGAGAGCAA ATGTCATTCA GCGCCGGGGC AGGGCAGGCC GCTGCCAGTC AGGTTTTGCC 
TACCACTTGT TCCCGAGGAG CCGGCTGGAG AAAATGGTTC CTTTCCAAGT GCCAGAGATC 
CTGCGCACAC CTCTTGAGAA CCTGGTGCTG CAAGCCAAAA TCCATATGCC TGAGAAGACG 
GCAGTGGAGT TCCTCTCTAA GGCTGTGGAC AGTCCAAATA TCAAGGCAGT GGATGAGGCC 
GTGATCCTGC TCCAGGAGAT TGGGGTGCTG GACCAGCGGG AGTATCTGAC CACCTTGGGA 
CAGCGCCTGG CCCACATCTC TACTGACCCC CGACTGGCCA AGGCCATAGT GTTGGCTGCC 
ATCTTCCGTT GCCTGCACCC ACTGCTGGTG GTTGTTTCCT GCCTTACCCG GGACCCCTTC 
AGCAGCAGTT TGCAGAACCG GGCAGAAGTA GACAAGGTGA AGGCATTGCT GAGCCATGAC 
AGTGGCAGTG ACCATTTGGC CTTCGTGCGG GCTGTGGCTG GCTGGGAGGA GGTACTGCGC 
TGGCAGGACC GTACCTCCAG GGAAAACTAC CTGGAAGAAA ACCTTCTGTA TGCCCCCAGC 
TTGCGCTTCA TCCACGGGCT CATCAAGCAG TTCTCAGAGA ACATTTATGA GGCTTTCCTA 
GTGGGGAAGC CCTCTGACTG CACACTGCCC TCTGCTCAGT GCAATGAGTA CAGCGAGGAA 
GAGGAGCTGG TGAAGGGTGT GCTGATGGCT GGCCTCTACC CCAACCTCAT CCAGGTGAGA 
CAAGGTAAGG TTACTCGGCA AGGCAAGTTC AAACCCAACA GTGTCACTTA CAGGACCAAA 
TCTGGCAACA TCTTGCTGCA TAAGTCAACC ATTAACAGGG AGGCTACCCG GTTACGGAGC 
CGATGGCTGA CATATTTCAT GGCCGTCAAG TCCAATGGTA GCGTCTTTGT TCGAGATTCC 
TCCCAGGTGC ACCCACTAGC TGTGTTGCTC CTAACAGATG GGGACGTGCA CATCCGAGAT 
GATGGGCGTC GGGCCACCAT CTCATTGAGT GACAGCGACC TGCTTCGGCT GGAAGGTGAT 
TCACGAACTG TGCGGTTGCT AAGGGAGTTT CGTCGAGCCC TAGGACGGAT GGTGGAGCGG 
AGCCTCCGCA GCGAGTTAGC TGCACTTCCT CTTAGTGTGC AGCAAGAACA CGGGCAGCTG 
CTTGCACTGC TGGCAGAGTT GCTTCGAGGA CCTTGTGGCA GCTTTGATAT GCGCAAGACA 
GCTGATGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_006512303.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512366.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X7, mRNA.

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CloneID	OMu12947
Clone ID Related Accession (Same CDS sequence)	XM_006512303.2 , XM_006512303.1 , XM_030244640.1 , XM_030244641.1 , XM_006512304.1 , XM_006512305.3 , NM_001252682.1 , XM_006512305.4
Accession Version	XM_006512303.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512303.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCT TGGACTCATT CAGAAAAGAT CGGACCCAGC ACAGGCAGCG TCAATGCAAG 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCACT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCGCAGCCTA AAAACCTTCT CAACAGCGTG 
ATTGGAAGAG CCCTTGGCAT CTCACATGCA AAAGACAAGT TAGTCTATGT GCACACGAAT 
GGACCGAAGA AAAAGAAAGT CACCCTGCAC ATAAAGTGGC CCAAGAGCGT GGAGGTGGAA 
GGCTATGGCA GCAAGAAGAT TGATGCTGAG CGTCAGGCTG CAGCAGCTGC CTGCCAACTC 
TTCAAGGGCT GGGGTCTGCT GGGACCACGG AATGAGCTGT TTGATGCAGC TAAATACCGA 
GTGCTAGCTG ATCGTTTTGG GTCTCCAGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCTCCAACTT CCTGGCGGCA GCTGAATCCT GAGAACATTC GGCCAGGGGG TCCTGCAGGA 
CTATCCCGAT CCTTAGGCCG AGAGGAAGAG GAAGATGAGG AGGAAGAGCT AGAAGAGGGG 
ACCATTGATG TGACAGAGTT TCTGTCTATG ACCCAGCAAG ACTCCCACAA CCCACTCAGG 
GACTCAAGGG GGGGCTCCTT TGAAATGACA GATGATGACA GTGCTATCAG AGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTCCTGGCC AAAGTGATTC AGATTGCAAC CTCCTCCTCC 
ACAGCTAAGA ATCTCATGCA GTTCCATACT GTGGGTACCA AGACCAAGCT GGCTACACTC 
ACTCTGCTCT GGCCCTGTCC CATGACCTTT GTTGCCAAAG GGCGACGCAA AGCTGAGGCT 
GAGAATAAGG CAGCAGCCTT GGCTTGCAAG AAACTGAAGA GCCTTGGCCT TGTGGACAGG 
AACAATGAGC CGCTTACCCA TGCCATGTAC AACCTGGCCT CCCTGCGTGA GTTGGGTGAG 
ACCCAGCGCC GGCCATGTAC CATCCAGGTG CCTGAGCCCA TCCTTCGCAA GATAGAGGCC 
TTCCTGAGTC ATTACCCGGT GGACAGCTCA TGGATTTCCC CAGAACTCCG ACTGCAGAGT 
GATGACATCT TGCCCTTAGG CAAGGACTCA GGGCCCTTGA GTGACCCTAT CACAGGCAAG 
CCATACATGC CCCTGTCAGA AGCAGAGGAG GTGCGCTTAA GCCAGAGCCT GCTAGAGCTG 
TGGCGGAGGA GAGGGCCGAT CTGGCAGGAG GCCCCGCAGC TACCTGTAGA CCCTCATCGG 
GACACTATCC TCAGTGCCAT TGAACAACAC CCAGTGGTAG TCATCTCTGG GGACACAGGC 
TGTGGGAAGA CCACACGTAT CCCTCAGCTG CTATTGGAGC GCTATGTGAC TGAGGGTCGA 
GGTGCCCGCT GCAATGTGAT CATCACACAA CCTCGCCGGA TCTCAGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCATGAACT GGGCCCCTCC TTGCGCCGGA ATGTGGGCTT CCAGGTACGC 
TTGGAAAGTA AGCCCCCAGC CCGAGGTGGG GCGCTGCTCT TCTGCACTGT GGGTATCCTG 
CTTCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGTGTGA GCCATGTCAT TGTGGATGAG 
GTCCATGAGC GGGATGTGAA CACAGACTTC CTGCTGATTC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCAG CTCTTCGGCT GGTGCTCATG AGTGCTACAG GAGATAATGA GCGCTTTTCC 
CGATACTTTG GGGGCTGCCC TGTTATCAAG GTACCTGGCT TCATGTATCC CGTCAAGGAA 
CACTACCTGG AGGACATCTT GGCCAAGCTG GGTAAACATC AGTACCCACA CCGGCATCGG 
CACCATGAGT CGGAAGATGA ATGTGCACTT GATTTGGACC TTGTGACTGA CCTGGTTCTG 
CATATCGATG CCCGTGGGGA ACCAGGTGGG ATCCTGTGCT TTCTACCTGG CTGGCAAGAA 
ATCAAAGGAG TGCAACAACG GCTCCAGGAG GCCCTGGGCA TGCATGAAAG CAAGTACCTC 
ATCTTACCAG TGCACTCCAA TATCCCCATG ATGGACCAGA AGGCCATATT CCAACAGCCT 
CCACTTGGGG TACGCAAGAT TGTATTGGCC ACCAACATTG CTGAAACCTC CATCACAGTT 
AACGACATTG TACATGTCGT GGACAGCGGT CTGCACAAGG AGGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACTGTGTGG GTGTCGAGAG CAAATGTCAT TCAGCGCCGG 
GGCAGGGCAG GCCGCTGCCA GTCAGGTTTT GCCTACCACT TGTTCCCGAG GAGCCGGCTG 
GAGAAAATGG TTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCCA AAATCCATAT GCCTGAGAAG ACGGCAGTGG AGTTCCTCTC TAAGGCTGTG 
GACAGTCCAA ATATCAAGGC AGTGGATGAG GCCGTGATCC TGCTCCAGGA GATTGGGGTG 
CTGGACCAGC GGGAGTATCT GACCACCTTG GGACAGCGCC TGGCCCACAT CTCTACTGAC 
CCCCGACTGG CCAAGGCCAT AGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTGCTG 
GTGGTTGTTT CCTGCCTTAC CCGGGACCCC TTCAGCAGCA GTTTGCAGAA CCGGGCAGAA 
GTAGACAAGG TGAAGGCATT GCTGAGCCAT GACAGTGGCA GTGACCATTT GGCCTTCGTG 
CGGGCTGTGG CTGGCTGGGA GGAGGTACTG CGCTGGCAGG ACCGTACCTC CAGGGAAAAC 
TACCTGGAAG AAAACCTTCT GTATGCCCCC AGCTTGCGCT TCATCCACGG GCTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTAGTGGGGA AGCCCTCTGA CTGCACACTG 
CCCTCTGCTC AGTGCAATGA GTACAGCGAG GAAGAGGAGC TGGTGAAGGG TGTGCTGATG 
GCTGGCCTCT ACCCCAACCT CATCCAGGTG AGACAAGGTA AGGTTACTCG GCAAGGCAAG 
TTCAAACCCA ACAGTGTCAC TTACAGGACC AAATCTGGCA ACATCTTGCT GCATAAGTCA 
ACCATTAACA GGGAGGCTAC CCGGTTACGG AGCCGATGGC TGACATATTT CATGGCCGTC 
AAGTCCAATG GTAGCGTCTT TGTTCGAGAT TCCTCCCAGG TGCACCCACT AGCTGTGTTG 
CTCCTAACAG ATGGGGACGT GCACATCCGA GATGATGGGC GTCGGGCCAC CATCTCATTG 
AGTGACAGCG ACCTGCTTCG GCTGGAAGGT GATTCACGAA CTGTGCGGTT GCTAAGGGAG 
TTTCGTCGAG CCCTAGGACG GATGGTGGAG CGGAGCCTCC GCAGCGAGTT AGCTGCACTT 
CCTCTTAGTG TGCAGCAAGA ACACGGGCAG CTGCTTGCAC TGCTGGCAGA GTTGCTTCGA 
GGACCTTGTG GCAGCTTTGA TATGCGCAAG ACAGCTGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512366.1
CDS	979..4563
Translation	MFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_006512303.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512303.2

ORF Insert Sequence:

ATGTTCAGCT TGGACTCATT CAGAAAAGAT CGGACCCAGC ACAGGCAGCG TCAATGCAAG 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCACT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCGCAGCCTA AAAACCTTCT CAACAGCGTG 
ATTGGAAGAG CCCTTGGCAT CTCACATGCA AAAGACAAGT TAGTCTATGT GCACACGAAT 
GGACCGAAGA AAAAGAAAGT CACCCTGCAC ATAAAGTGGC CCAAGAGCGT GGAGGTGGAA 
GGCTATGGCA GCAAGAAGAT TGATGCTGAG CGTCAGGCTG CAGCAGCTGC CTGCCAACTC 
TTCAAGGGCT GGGGTCTGCT GGGACCACGG AATGAGCTGT TTGATGCAGC TAAATACCGA 
GTGCTAGCTG ATCGTTTTGG GTCTCCAGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCTCCAACTT CCTGGCGGCA GCTGAATCCT GAGAACATTC GGCCAGGGGG TCCTGCAGGA 
CTATCCCGAT CCTTAGGCCG AGAGGAAGAG GAAGATGAGG AGGAAGAGCT AGAAGAGGGG 
ACCATTGATG TGACAGAGTT TCTGTCTATG ACCCAGCAAG ACTCCCACAA CCCACTCAGG 
GACTCAAGGG GGGGCTCCTT TGAAATGACA GATGATGACA GTGCTATCAG AGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTCCTGGCC AAAGTGATTC AGATTGCAAC CTCCTCCTCC 
ACAGCTAAGA ATCTCATGCA GTTCCATACT GTGGGTACCA AGACCAAGCT GGCTACACTC 
ACTCTGCTCT GGCCCTGTCC CATGACCTTT GTTGCCAAAG GGCGACGCAA AGCTGAGGCT 
GAGAATAAGG CAGCAGCCTT GGCTTGCAAG AAACTGAAGA GCCTTGGCCT TGTGGACAGG 
AACAATGAGC CGCTTACCCA TGCCATGTAC AACCTGGCCT CCCTGCGTGA GTTGGGTGAG 
ACCCAGCGCC GGCCATGTAC CATCCAGGTG CCTGAGCCCA TCCTTCGCAA GATAGAGGCC 
TTCCTGAGTC ATTACCCGGT GGACAGCTCA TGGATTTCCC CAGAACTCCG ACTGCAGAGT 
GATGACATCT TGCCCTTAGG CAAGGACTCA GGGCCCTTGA GTGACCCTAT CACAGGCAAG 
CCATACATGC CCCTGTCAGA AGCAGAGGAG GTGCGCTTAA GCCAGAGCCT GCTAGAGCTG 
TGGCGGAGGA GAGGGCCGAT CTGGCAGGAG GCCCCGCAGC TACCTGTAGA CCCTCATCGG 
GACACTATCC TCAGTGCCAT TGAACAACAC CCAGTGGTAG TCATCTCTGG GGACACAGGC 
TGTGGGAAGA CCACACGTAT CCCTCAGCTG CTATTGGAGC GCTATGTGAC TGAGGGTCGA 
GGTGCCCGCT GCAATGTGAT CATCACACAA CCTCGCCGGA TCTCAGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCATGAACT GGGCCCCTCC TTGCGCCGGA ATGTGGGCTT CCAGGTACGC 
TTGGAAAGTA AGCCCCCAGC CCGAGGTGGG GCGCTGCTCT TCTGCACTGT GGGTATCCTG 
CTTCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGTGTGA GCCATGTCAT TGTGGATGAG 
GTCCATGAGC GGGATGTGAA CACAGACTTC CTGCTGATTC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCAG CTCTTCGGCT GGTGCTCATG AGTGCTACAG GAGATAATGA GCGCTTTTCC 
CGATACTTTG GGGGCTGCCC TGTTATCAAG GTACCTGGCT TCATGTATCC CGTCAAGGAA 
CACTACCTGG AGGACATCTT GGCCAAGCTG GGTAAACATC AGTACCCACA CCGGCATCGG 
CACCATGAGT CGGAAGATGA ATGTGCACTT GATTTGGACC TTGTGACTGA CCTGGTTCTG 
CATATCGATG CCCGTGGGGA ACCAGGTGGG ATCCTGTGCT TTCTACCTGG CTGGCAAGAA 
ATCAAAGGAG TGCAACAACG GCTCCAGGAG GCCCTGGGCA TGCATGAAAG CAAGTACCTC 
ATCTTACCAG TGCACTCCAA TATCCCCATG ATGGACCAGA AGGCCATATT CCAACAGCCT 
CCACTTGGGG TACGCAAGAT TGTATTGGCC ACCAACATTG CTGAAACCTC CATCACAGTT 
AACGACATTG TACATGTCGT GGACAGCGGT CTGCACAAGG AGGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACTGTGTGG GTGTCGAGAG CAAATGTCAT TCAGCGCCGG 
GGCAGGGCAG GCCGCTGCCA GTCAGGTTTT GCCTACCACT TGTTCCCGAG GAGCCGGCTG 
GAGAAAATGG TTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCCA AAATCCATAT GCCTGAGAAG ACGGCAGTGG AGTTCCTCTC TAAGGCTGTG 
GACAGTCCAA ATATCAAGGC AGTGGATGAG GCCGTGATCC TGCTCCAGGA GATTGGGGTG 
CTGGACCAGC GGGAGTATCT GACCACCTTG GGACAGCGCC TGGCCCACAT CTCTACTGAC 
CCCCGACTGG CCAAGGCCAT AGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTGCTG 
GTGGTTGTTT CCTGCCTTAC CCGGGACCCC TTCAGCAGCA GTTTGCAGAA CCGGGCAGAA 
GTAGACAAGG TGAAGGCATT GCTGAGCCAT GACAGTGGCA GTGACCATTT GGCCTTCGTG 
CGGGCTGTGG CTGGCTGGGA GGAGGTACTG CGCTGGCAGG ACCGTACCTC CAGGGAAAAC 
TACCTGGAAG AAAACCTTCT GTATGCCCCC AGCTTGCGCT TCATCCACGG GCTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTAGTGGGGA AGCCCTCTGA CTGCACACTG 
CCCTCTGCTC AGTGCAATGA GTACAGCGAG GAAGAGGAGC TGGTGAAGGG TGTGCTGATG 
GCTGGCCTCT ACCCCAACCT CATCCAGGTG AGACAAGGTA AGGTTACTCG GCAAGGCAAG 
TTCAAACCCA ACAGTGTCAC TTACAGGACC AAATCTGGCA ACATCTTGCT GCATAAGTCA 
ACCATTAACA GGGAGGCTAC CCGGTTACGG AGCCGATGGC TGACATATTT CATGGCCGTC 
AAGTCCAATG GTAGCGTCTT TGTTCGAGAT TCCTCCCAGG TGCACCCACT AGCTGTGTTG 
CTCCTAACAG ATGGGGACGT GCACATCCGA GATGATGGGC GTCGGGCCAC CATCTCATTG 
AGTGACAGCG ACCTGCTTCG GCTGGAAGGT GATTCACGAA CTGTGCGGTT GCTAAGGGAG 
TTTCGTCGAG CCCTAGGACG GATGGTGGAG CGGAGCCTCC GCAGCGAGTT AGCTGCACTT 
CCTCTTAGTG TGCAGCAAGA ACACGGGCAG CTGCTTGCAC TGCTGGCAGA GTTGCTTCGA 
GGACCTTGTG GCAGCTTTGA TATGCGCAAG ACAGCTGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_030244640.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100500.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X8, mRNA.

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CloneID	OMu12947
Clone ID Related Accession (Same CDS sequence)	XM_006512303.2 , XM_006512303.1 , XM_030244640.1 , XM_030244641.1 , XM_006512304.1 , XM_006512305.3 , NM_001252682.1 , XM_006512305.4
Accession Version	XM_030244640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCT TGGACTCATT CAGAAAAGAT CGGACCCAGC ACAGGCAGCG TCAATGCAAG 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCACT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCGCAGCCTA AAAACCTTCT CAACAGCGTG 
ATTGGAAGAG CCCTTGGCAT CTCACATGCA AAAGACAAGT TAGTCTATGT GCACACGAAT 
GGACCGAAGA AAAAGAAAGT CACCCTGCAC ATAAAGTGGC CCAAGAGCGT GGAGGTGGAA 
GGCTATGGCA GCAAGAAGAT TGATGCTGAG CGTCAGGCTG CAGCAGCTGC CTGCCAACTC 
TTCAAGGGCT GGGGTCTGCT GGGACCACGG AATGAGCTGT TTGATGCAGC TAAATACCGA 
GTGCTAGCTG ATCGTTTTGG GTCTCCAGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCTCCAACTT CCTGGCGGCA GCTGAATCCT GAGAACATTC GGCCAGGGGG TCCTGCAGGA 
CTATCCCGAT CCTTAGGCCG AGAGGAAGAG GAAGATGAGG AGGAAGAGCT AGAAGAGGGG 
ACCATTGATG TGACAGAGTT TCTGTCTATG ACCCAGCAAG ACTCCCACAA CCCACTCAGG 
GACTCAAGGG GGGGCTCCTT TGAAATGACA GATGATGACA GTGCTATCAG AGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTCCTGGCC AAAGTGATTC AGATTGCAAC CTCCTCCTCC 
ACAGCTAAGA ATCTCATGCA GTTCCATACT GTGGGTACCA AGACCAAGCT GGCTACACTC 
ACTCTGCTCT GGCCCTGTCC CATGACCTTT GTTGCCAAAG GGCGACGCAA AGCTGAGGCT 
GAGAATAAGG CAGCAGCCTT GGCTTGCAAG AAACTGAAGA GCCTTGGCCT TGTGGACAGG 
AACAATGAGC CGCTTACCCA TGCCATGTAC AACCTGGCCT CCCTGCGTGA GTTGGGTGAG 
ACCCAGCGCC GGCCATGTAC CATCCAGGTG CCTGAGCCCA TCCTTCGCAA GATAGAGGCC 
TTCCTGAGTC ATTACCCGGT GGACAGCTCA TGGATTTCCC CAGAACTCCG ACTGCAGAGT 
GATGACATCT TGCCCTTAGG CAAGGACTCA GGGCCCTTGA GTGACCCTAT CACAGGCAAG 
CCATACATGC CCCTGTCAGA AGCAGAGGAG GTGCGCTTAA GCCAGAGCCT GCTAGAGCTG 
TGGCGGAGGA GAGGGCCGAT CTGGCAGGAG GCCCCGCAGC TACCTGTAGA CCCTCATCGG 
GACACTATCC TCAGTGCCAT TGAACAACAC CCAGTGGTAG TCATCTCTGG GGACACAGGC 
TGTGGGAAGA CCACACGTAT CCCTCAGCTG CTATTGGAGC GCTATGTGAC TGAGGGTCGA 
GGTGCCCGCT GCAATGTGAT CATCACACAA CCTCGCCGGA TCTCAGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCATGAACT GGGCCCCTCC TTGCGCCGGA ATGTGGGCTT CCAGGTACGC 
TTGGAAAGTA AGCCCCCAGC CCGAGGTGGG GCGCTGCTCT TCTGCACTGT GGGTATCCTG 
CTTCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGTGTGA GCCATGTCAT TGTGGATGAG 
GTCCATGAGC GGGATGTGAA CACAGACTTC CTGCTGATTC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCAG CTCTTCGGCT GGTGCTCATG AGTGCTACAG GAGATAATGA GCGCTTTTCC 
CGATACTTTG GGGGCTGCCC TGTTATCAAG GTACCTGGCT TCATGTATCC CGTCAAGGAA 
CACTACCTGG AGGACATCTT GGCCAAGCTG GGTAAACATC AGTACCCACA CCGGCATCGG 
CACCATGAGT CGGAAGATGA ATGTGCACTT GATTTGGACC TTGTGACTGA CCTGGTTCTG 
CATATCGATG CCCGTGGGGA ACCAGGTGGG ATCCTGTGCT TTCTACCTGG CTGGCAAGAA 
ATCAAAGGAG TGCAACAACG GCTCCAGGAG GCCCTGGGCA TGCATGAAAG CAAGTACCTC 
ATCTTACCAG TGCACTCCAA TATCCCCATG ATGGACCAGA AGGCCATATT CCAACAGCCT 
CCACTTGGGG TACGCAAGAT TGTATTGGCC ACCAACATTG CTGAAACCTC CATCACAGTT 
AACGACATTG TACATGTCGT GGACAGCGGT CTGCACAAGG AGGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACTGTGTGG GTGTCGAGAG CAAATGTCAT TCAGCGCCGG 
GGCAGGGCAG GCCGCTGCCA GTCAGGTTTT GCCTACCACT TGTTCCCGAG GAGCCGGCTG 
GAGAAAATGG TTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCCA AAATCCATAT GCCTGAGAAG ACGGCAGTGG AGTTCCTCTC TAAGGCTGTG 
GACAGTCCAA ATATCAAGGC AGTGGATGAG GCCGTGATCC TGCTCCAGGA GATTGGGGTG 
CTGGACCAGC GGGAGTATCT GACCACCTTG GGACAGCGCC TGGCCCACAT CTCTACTGAC 
CCCCGACTGG CCAAGGCCAT AGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTGCTG 
GTGGTTGTTT CCTGCCTTAC CCGGGACCCC TTCAGCAGCA GTTTGCAGAA CCGGGCAGAA 
GTAGACAAGG TGAAGGCATT GCTGAGCCAT GACAGTGGCA GTGACCATTT GGCCTTCGTG 
CGGGCTGTGG CTGGCTGGGA GGAGGTACTG CGCTGGCAGG ACCGTACCTC CAGGGAAAAC 
TACCTGGAAG AAAACCTTCT GTATGCCCCC AGCTTGCGCT TCATCCACGG GCTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTAGTGGGGA AGCCCTCTGA CTGCACACTG 
CCCTCTGCTC AGTGCAATGA GTACAGCGAG GAAGAGGAGC TGGTGAAGGG TGTGCTGATG 
GCTGGCCTCT ACCCCAACCT CATCCAGGTG AGACAAGGTA AGGTTACTCG GCAAGGCAAG 
TTCAAACCCA ACAGTGTCAC TTACAGGACC AAATCTGGCA ACATCTTGCT GCATAAGTCA 
ACCATTAACA GGGAGGCTAC CCGGTTACGG AGCCGATGGC TGACATATTT CATGGCCGTC 
AAGTCCAATG GTAGCGTCTT TGTTCGAGAT TCCTCCCAGG TGCACCCACT AGCTGTGTTG 
CTCCTAACAG ATGGGGACGT GCACATCCGA GATGATGGGC GTCGGGCCAC CATCTCATTG 
AGTGACAGCG ACCTGCTTCG GCTGGAAGGT GATTCACGAA CTGTGCGGTT GCTAAGGGAG 
TTTCGTCGAG CCCTAGGACG GATGGTGGAG CGGAGCCTCC GCAGCGAGTT AGCTGCACTT 
CCTCTTAGTG TGCAGCAAGA ACACGGGCAG CTGCTTGCAC TGCTGGCAGA GTTGCTTCGA 
GGACCTTGTG GCAGCTTTGA TATGCGCAAG ACAGCTGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100500.1
CDS	502..4086
Translation	MFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_030244640.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244640.1

ORF Insert Sequence:

ATGTTCAGCT TGGACTCATT CAGAAAAGAT CGGACCCAGC ACAGGCAGCG TCAATGCAAG 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCACT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCGCAGCCTA AAAACCTTCT CAACAGCGTG 
ATTGGAAGAG CCCTTGGCAT CTCACATGCA AAAGACAAGT TAGTCTATGT GCACACGAAT 
GGACCGAAGA AAAAGAAAGT CACCCTGCAC ATAAAGTGGC CCAAGAGCGT GGAGGTGGAA 
GGCTATGGCA GCAAGAAGAT TGATGCTGAG CGTCAGGCTG CAGCAGCTGC CTGCCAACTC 
TTCAAGGGCT GGGGTCTGCT GGGACCACGG AATGAGCTGT TTGATGCAGC TAAATACCGA 
GTGCTAGCTG ATCGTTTTGG GTCTCCAGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCTCCAACTT CCTGGCGGCA GCTGAATCCT GAGAACATTC GGCCAGGGGG TCCTGCAGGA 
CTATCCCGAT CCTTAGGCCG AGAGGAAGAG GAAGATGAGG AGGAAGAGCT AGAAGAGGGG 
ACCATTGATG TGACAGAGTT TCTGTCTATG ACCCAGCAAG ACTCCCACAA CCCACTCAGG 
GACTCAAGGG GGGGCTCCTT TGAAATGACA GATGATGACA GTGCTATCAG AGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTCCTGGCC AAAGTGATTC AGATTGCAAC CTCCTCCTCC 
ACAGCTAAGA ATCTCATGCA GTTCCATACT GTGGGTACCA AGACCAAGCT GGCTACACTC 
ACTCTGCTCT GGCCCTGTCC CATGACCTTT GTTGCCAAAG GGCGACGCAA AGCTGAGGCT 
GAGAATAAGG CAGCAGCCTT GGCTTGCAAG AAACTGAAGA GCCTTGGCCT TGTGGACAGG 
AACAATGAGC CGCTTACCCA TGCCATGTAC AACCTGGCCT CCCTGCGTGA GTTGGGTGAG 
ACCCAGCGCC GGCCATGTAC CATCCAGGTG CCTGAGCCCA TCCTTCGCAA GATAGAGGCC 
TTCCTGAGTC ATTACCCGGT GGACAGCTCA TGGATTTCCC CAGAACTCCG ACTGCAGAGT 
GATGACATCT TGCCCTTAGG CAAGGACTCA GGGCCCTTGA GTGACCCTAT CACAGGCAAG 
CCATACATGC CCCTGTCAGA AGCAGAGGAG GTGCGCTTAA GCCAGAGCCT GCTAGAGCTG 
TGGCGGAGGA GAGGGCCGAT CTGGCAGGAG GCCCCGCAGC TACCTGTAGA CCCTCATCGG 
GACACTATCC TCAGTGCCAT TGAACAACAC CCAGTGGTAG TCATCTCTGG GGACACAGGC 
TGTGGGAAGA CCACACGTAT CCCTCAGCTG CTATTGGAGC GCTATGTGAC TGAGGGTCGA 
GGTGCCCGCT GCAATGTGAT CATCACACAA CCTCGCCGGA TCTCAGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCATGAACT GGGCCCCTCC TTGCGCCGGA ATGTGGGCTT CCAGGTACGC 
TTGGAAAGTA AGCCCCCAGC CCGAGGTGGG GCGCTGCTCT TCTGCACTGT GGGTATCCTG 
CTTCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGTGTGA GCCATGTCAT TGTGGATGAG 
GTCCATGAGC GGGATGTGAA CACAGACTTC CTGCTGATTC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCAG CTCTTCGGCT GGTGCTCATG AGTGCTACAG GAGATAATGA GCGCTTTTCC 
CGATACTTTG GGGGCTGCCC TGTTATCAAG GTACCTGGCT TCATGTATCC CGTCAAGGAA 
CACTACCTGG AGGACATCTT GGCCAAGCTG GGTAAACATC AGTACCCACA CCGGCATCGG 
CACCATGAGT CGGAAGATGA ATGTGCACTT GATTTGGACC TTGTGACTGA CCTGGTTCTG 
CATATCGATG CCCGTGGGGA ACCAGGTGGG ATCCTGTGCT TTCTACCTGG CTGGCAAGAA 
ATCAAAGGAG TGCAACAACG GCTCCAGGAG GCCCTGGGCA TGCATGAAAG CAAGTACCTC 
ATCTTACCAG TGCACTCCAA TATCCCCATG ATGGACCAGA AGGCCATATT CCAACAGCCT 
CCACTTGGGG TACGCAAGAT TGTATTGGCC ACCAACATTG CTGAAACCTC CATCACAGTT 
AACGACATTG TACATGTCGT GGACAGCGGT CTGCACAAGG AGGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACTGTGTGG GTGTCGAGAG CAAATGTCAT TCAGCGCCGG 
GGCAGGGCAG GCCGCTGCCA GTCAGGTTTT GCCTACCACT TGTTCCCGAG GAGCCGGCTG 
GAGAAAATGG TTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCCA AAATCCATAT GCCTGAGAAG ACGGCAGTGG AGTTCCTCTC TAAGGCTGTG 
GACAGTCCAA ATATCAAGGC AGTGGATGAG GCCGTGATCC TGCTCCAGGA GATTGGGGTG 
CTGGACCAGC GGGAGTATCT GACCACCTTG GGACAGCGCC TGGCCCACAT CTCTACTGAC 
CCCCGACTGG CCAAGGCCAT AGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTGCTG 
GTGGTTGTTT CCTGCCTTAC CCGGGACCCC TTCAGCAGCA GTTTGCAGAA CCGGGCAGAA 
GTAGACAAGG TGAAGGCATT GCTGAGCCAT GACAGTGGCA GTGACCATTT GGCCTTCGTG 
CGGGCTGTGG CTGGCTGGGA GGAGGTACTG CGCTGGCAGG ACCGTACCTC CAGGGAAAAC 
TACCTGGAAG AAAACCTTCT GTATGCCCCC AGCTTGCGCT TCATCCACGG GCTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTAGTGGGGA AGCCCTCTGA CTGCACACTG 
CCCTCTGCTC AGTGCAATGA GTACAGCGAG GAAGAGGAGC TGGTGAAGGG TGTGCTGATG 
GCTGGCCTCT ACCCCAACCT CATCCAGGTG AGACAAGGTA AGGTTACTCG GCAAGGCAAG 
TTCAAACCCA ACAGTGTCAC TTACAGGACC AAATCTGGCA ACATCTTGCT GCATAAGTCA 
ACCATTAACA GGGAGGCTAC CCGGTTACGG AGCCGATGGC TGACATATTT CATGGCCGTC 
AAGTCCAATG GTAGCGTCTT TGTTCGAGAT TCCTCCCAGG TGCACCCACT AGCTGTGTTG 
CTCCTAACAG ATGGGGACGT GCACATCCGA GATGATGGGC GTCGGGCCAC CATCTCATTG 
AGTGACAGCG ACCTGCTTCG GCTGGAAGGT GATTCACGAA CTGTGCGGTT GCTAAGGGAG 
TTTCGTCGAG CCCTAGGACG GATGGTGGAG CGGAGCCTCC GCAGCGAGTT AGCTGCACTT 
CCTCTTAGTG TGCAGCAAGA ACACGGGCAG CTGCTTGCAC TGCTGGCAGA GTTGCTTCGA 
GGACCTTGTG GCAGCTTTGA TATGCGCAAG ACAGCTGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_030244641.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100501.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X9, mRNA.

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CloneID	OMu12947
Clone ID Related Accession (Same CDS sequence)	XM_006512303.2 , XM_006512303.1 , XM_030244640.1 , XM_030244641.1 , XM_006512304.1 , XM_006512305.3 , NM_001252682.1 , XM_006512305.4
Accession Version	XM_030244641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCT TGGACTCATT CAGAAAAGAT CGGACCCAGC ACAGGCAGCG TCAATGCAAG 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCACT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCGCAGCCTA AAAACCTTCT CAACAGCGTG 
ATTGGAAGAG CCCTTGGCAT CTCACATGCA AAAGACAAGT TAGTCTATGT GCACACGAAT 
GGACCGAAGA AAAAGAAAGT CACCCTGCAC ATAAAGTGGC CCAAGAGCGT GGAGGTGGAA 
GGCTATGGCA GCAAGAAGAT TGATGCTGAG CGTCAGGCTG CAGCAGCTGC CTGCCAACTC 
TTCAAGGGCT GGGGTCTGCT GGGACCACGG AATGAGCTGT TTGATGCAGC TAAATACCGA 
GTGCTAGCTG ATCGTTTTGG GTCTCCAGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCTCCAACTT CCTGGCGGCA GCTGAATCCT GAGAACATTC GGCCAGGGGG TCCTGCAGGA 
CTATCCCGAT CCTTAGGCCG AGAGGAAGAG GAAGATGAGG AGGAAGAGCT AGAAGAGGGG 
ACCATTGATG TGACAGAGTT TCTGTCTATG ACCCAGCAAG ACTCCCACAA CCCACTCAGG 
GACTCAAGGG GGGGCTCCTT TGAAATGACA GATGATGACA GTGCTATCAG AGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTCCTGGCC AAAGTGATTC AGATTGCAAC CTCCTCCTCC 
ACAGCTAAGA ATCTCATGCA GTTCCATACT GTGGGTACCA AGACCAAGCT GGCTACACTC 
ACTCTGCTCT GGCCCTGTCC CATGACCTTT GTTGCCAAAG GGCGACGCAA AGCTGAGGCT 
GAGAATAAGG CAGCAGCCTT GGCTTGCAAG AAACTGAAGA GCCTTGGCCT TGTGGACAGG 
AACAATGAGC CGCTTACCCA TGCCATGTAC AACCTGGCCT CCCTGCGTGA GTTGGGTGAG 
ACCCAGCGCC GGCCATGTAC CATCCAGGTG CCTGAGCCCA TCCTTCGCAA GATAGAGGCC 
TTCCTGAGTC ATTACCCGGT GGACAGCTCA TGGATTTCCC CAGAACTCCG ACTGCAGAGT 
GATGACATCT TGCCCTTAGG CAAGGACTCA GGGCCCTTGA GTGACCCTAT CACAGGCAAG 
CCATACATGC CCCTGTCAGA AGCAGAGGAG GTGCGCTTAA GCCAGAGCCT GCTAGAGCTG 
TGGCGGAGGA GAGGGCCGAT CTGGCAGGAG GCCCCGCAGC TACCTGTAGA CCCTCATCGG 
GACACTATCC TCAGTGCCAT TGAACAACAC CCAGTGGTAG TCATCTCTGG GGACACAGGC 
TGTGGGAAGA CCACACGTAT CCCTCAGCTG CTATTGGAGC GCTATGTGAC TGAGGGTCGA 
GGTGCCCGCT GCAATGTGAT CATCACACAA CCTCGCCGGA TCTCAGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCATGAACT GGGCCCCTCC TTGCGCCGGA ATGTGGGCTT CCAGGTACGC 
TTGGAAAGTA AGCCCCCAGC CCGAGGTGGG GCGCTGCTCT TCTGCACTGT GGGTATCCTG 
CTTCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGTGTGA GCCATGTCAT TGTGGATGAG 
GTCCATGAGC GGGATGTGAA CACAGACTTC CTGCTGATTC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCAG CTCTTCGGCT GGTGCTCATG AGTGCTACAG GAGATAATGA GCGCTTTTCC 
CGATACTTTG GGGGCTGCCC TGTTATCAAG GTACCTGGCT TCATGTATCC CGTCAAGGAA 
CACTACCTGG AGGACATCTT GGCCAAGCTG GGTAAACATC AGTACCCACA CCGGCATCGG 
CACCATGAGT CGGAAGATGA ATGTGCACTT GATTTGGACC TTGTGACTGA CCTGGTTCTG 
CATATCGATG CCCGTGGGGA ACCAGGTGGG ATCCTGTGCT TTCTACCTGG CTGGCAAGAA 
ATCAAAGGAG TGCAACAACG GCTCCAGGAG GCCCTGGGCA TGCATGAAAG CAAGTACCTC 
ATCTTACCAG TGCACTCCAA TATCCCCATG ATGGACCAGA AGGCCATATT CCAACAGCCT 
CCACTTGGGG TACGCAAGAT TGTATTGGCC ACCAACATTG CTGAAACCTC CATCACAGTT 
AACGACATTG TACATGTCGT GGACAGCGGT CTGCACAAGG AGGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACTGTGTGG GTGTCGAGAG CAAATGTCAT TCAGCGCCGG 
GGCAGGGCAG GCCGCTGCCA GTCAGGTTTT GCCTACCACT TGTTCCCGAG GAGCCGGCTG 
GAGAAAATGG TTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCCA AAATCCATAT GCCTGAGAAG ACGGCAGTGG AGTTCCTCTC TAAGGCTGTG 
GACAGTCCAA ATATCAAGGC AGTGGATGAG GCCGTGATCC TGCTCCAGGA GATTGGGGTG 
CTGGACCAGC GGGAGTATCT GACCACCTTG GGACAGCGCC TGGCCCACAT CTCTACTGAC 
CCCCGACTGG CCAAGGCCAT AGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTGCTG 
GTGGTTGTTT CCTGCCTTAC CCGGGACCCC TTCAGCAGCA GTTTGCAGAA CCGGGCAGAA 
GTAGACAAGG TGAAGGCATT GCTGAGCCAT GACAGTGGCA GTGACCATTT GGCCTTCGTG 
CGGGCTGTGG CTGGCTGGGA GGAGGTACTG CGCTGGCAGG ACCGTACCTC CAGGGAAAAC 
TACCTGGAAG AAAACCTTCT GTATGCCCCC AGCTTGCGCT TCATCCACGG GCTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTAGTGGGGA AGCCCTCTGA CTGCACACTG 
CCCTCTGCTC AGTGCAATGA GTACAGCGAG GAAGAGGAGC TGGTGAAGGG TGTGCTGATG 
GCTGGCCTCT ACCCCAACCT CATCCAGGTG AGACAAGGTA AGGTTACTCG GCAAGGCAAG 
TTCAAACCCA ACAGTGTCAC TTACAGGACC AAATCTGGCA ACATCTTGCT GCATAAGTCA 
ACCATTAACA GGGAGGCTAC CCGGTTACGG AGCCGATGGC TGACATATTT CATGGCCGTC 
AAGTCCAATG GTAGCGTCTT TGTTCGAGAT TCCTCCCAGG TGCACCCACT AGCTGTGTTG 
CTCCTAACAG ATGGGGACGT GCACATCCGA GATGATGGGC GTCGGGCCAC CATCTCATTG 
AGTGACAGCG ACCTGCTTCG GCTGGAAGGT GATTCACGAA CTGTGCGGTT GCTAAGGGAG 
TTTCGTCGAG CCCTAGGACG GATGGTGGAG CGGAGCCTCC GCAGCGAGTT AGCTGCACTT 
CCTCTTAGTG TGCAGCAAGA ACACGGGCAG CTGCTTGCAC TGCTGGCAGA GTTGCTTCGA 
GGACCTTGTG GCAGCTTTGA TATGCGCAAG ACAGCTGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100501.1
CDS	969..4553
Translation	MFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_030244641.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244641.1

ORF Insert Sequence:

ATGTTCAGCT TGGACTCATT CAGAAAAGAT CGGACCCAGC ACAGGCAGCG TCAATGCAAG 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCACT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCGCAGCCTA AAAACCTTCT CAACAGCGTG 
ATTGGAAGAG CCCTTGGCAT CTCACATGCA AAAGACAAGT TAGTCTATGT GCACACGAAT 
GGACCGAAGA AAAAGAAAGT CACCCTGCAC ATAAAGTGGC CCAAGAGCGT GGAGGTGGAA 
GGCTATGGCA GCAAGAAGAT TGATGCTGAG CGTCAGGCTG CAGCAGCTGC CTGCCAACTC 
TTCAAGGGCT GGGGTCTGCT GGGACCACGG AATGAGCTGT TTGATGCAGC TAAATACCGA 
GTGCTAGCTG ATCGTTTTGG GTCTCCAGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCTCCAACTT CCTGGCGGCA GCTGAATCCT GAGAACATTC GGCCAGGGGG TCCTGCAGGA 
CTATCCCGAT CCTTAGGCCG AGAGGAAGAG GAAGATGAGG AGGAAGAGCT AGAAGAGGGG 
ACCATTGATG TGACAGAGTT TCTGTCTATG ACCCAGCAAG ACTCCCACAA CCCACTCAGG 
GACTCAAGGG GGGGCTCCTT TGAAATGACA GATGATGACA GTGCTATCAG AGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTCCTGGCC AAAGTGATTC AGATTGCAAC CTCCTCCTCC 
ACAGCTAAGA ATCTCATGCA GTTCCATACT GTGGGTACCA AGACCAAGCT GGCTACACTC 
ACTCTGCTCT GGCCCTGTCC CATGACCTTT GTTGCCAAAG GGCGACGCAA AGCTGAGGCT 
GAGAATAAGG CAGCAGCCTT GGCTTGCAAG AAACTGAAGA GCCTTGGCCT TGTGGACAGG 
AACAATGAGC CGCTTACCCA TGCCATGTAC AACCTGGCCT CCCTGCGTGA GTTGGGTGAG 
ACCCAGCGCC GGCCATGTAC CATCCAGGTG CCTGAGCCCA TCCTTCGCAA GATAGAGGCC 
TTCCTGAGTC ATTACCCGGT GGACAGCTCA TGGATTTCCC CAGAACTCCG ACTGCAGAGT 
GATGACATCT TGCCCTTAGG CAAGGACTCA GGGCCCTTGA GTGACCCTAT CACAGGCAAG 
CCATACATGC CCCTGTCAGA AGCAGAGGAG GTGCGCTTAA GCCAGAGCCT GCTAGAGCTG 
TGGCGGAGGA GAGGGCCGAT CTGGCAGGAG GCCCCGCAGC TACCTGTAGA CCCTCATCGG 
GACACTATCC TCAGTGCCAT TGAACAACAC CCAGTGGTAG TCATCTCTGG GGACACAGGC 
TGTGGGAAGA CCACACGTAT CCCTCAGCTG CTATTGGAGC GCTATGTGAC TGAGGGTCGA 
GGTGCCCGCT GCAATGTGAT CATCACACAA CCTCGCCGGA TCTCAGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCATGAACT GGGCCCCTCC TTGCGCCGGA ATGTGGGCTT CCAGGTACGC 
TTGGAAAGTA AGCCCCCAGC CCGAGGTGGG GCGCTGCTCT TCTGCACTGT GGGTATCCTG 
CTTCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGTGTGA GCCATGTCAT TGTGGATGAG 
GTCCATGAGC GGGATGTGAA CACAGACTTC CTGCTGATTC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCAG CTCTTCGGCT GGTGCTCATG AGTGCTACAG GAGATAATGA GCGCTTTTCC 
CGATACTTTG GGGGCTGCCC TGTTATCAAG GTACCTGGCT TCATGTATCC CGTCAAGGAA 
CACTACCTGG AGGACATCTT GGCCAAGCTG GGTAAACATC AGTACCCACA CCGGCATCGG 
CACCATGAGT CGGAAGATGA ATGTGCACTT GATTTGGACC TTGTGACTGA CCTGGTTCTG 
CATATCGATG CCCGTGGGGA ACCAGGTGGG ATCCTGTGCT TTCTACCTGG CTGGCAAGAA 
ATCAAAGGAG TGCAACAACG GCTCCAGGAG GCCCTGGGCA TGCATGAAAG CAAGTACCTC 
ATCTTACCAG TGCACTCCAA TATCCCCATG ATGGACCAGA AGGCCATATT CCAACAGCCT 
CCACTTGGGG TACGCAAGAT TGTATTGGCC ACCAACATTG CTGAAACCTC CATCACAGTT 
AACGACATTG TACATGTCGT GGACAGCGGT CTGCACAAGG AGGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACTGTGTGG GTGTCGAGAG CAAATGTCAT TCAGCGCCGG 
GGCAGGGCAG GCCGCTGCCA GTCAGGTTTT GCCTACCACT TGTTCCCGAG GAGCCGGCTG 
GAGAAAATGG TTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCCA AAATCCATAT GCCTGAGAAG ACGGCAGTGG AGTTCCTCTC TAAGGCTGTG 
GACAGTCCAA ATATCAAGGC AGTGGATGAG GCCGTGATCC TGCTCCAGGA GATTGGGGTG 
CTGGACCAGC GGGAGTATCT GACCACCTTG GGACAGCGCC TGGCCCACAT CTCTACTGAC 
CCCCGACTGG CCAAGGCCAT AGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTGCTG 
GTGGTTGTTT CCTGCCTTAC CCGGGACCCC TTCAGCAGCA GTTTGCAGAA CCGGGCAGAA 
GTAGACAAGG TGAAGGCATT GCTGAGCCAT GACAGTGGCA GTGACCATTT GGCCTTCGTG 
CGGGCTGTGG CTGGCTGGGA GGAGGTACTG CGCTGGCAGG ACCGTACCTC CAGGGAAAAC 
TACCTGGAAG AAAACCTTCT GTATGCCCCC AGCTTGCGCT TCATCCACGG GCTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTAGTGGGGA AGCCCTCTGA CTGCACACTG 
CCCTCTGCTC AGTGCAATGA GTACAGCGAG GAAGAGGAGC TGGTGAAGGG TGTGCTGATG 
GCTGGCCTCT ACCCCAACCT CATCCAGGTG AGACAAGGTA AGGTTACTCG GCAAGGCAAG 
TTCAAACCCA ACAGTGTCAC TTACAGGACC AAATCTGGCA ACATCTTGCT GCATAAGTCA 
ACCATTAACA GGGAGGCTAC CCGGTTACGG AGCCGATGGC TGACATATTT CATGGCCGTC 
AAGTCCAATG GTAGCGTCTT TGTTCGAGAT TCCTCCCAGG TGCACCCACT AGCTGTGTTG 
CTCCTAACAG ATGGGGACGT GCACATCCGA GATGATGGGC GTCGGGCCAC CATCTCATTG 
AGTGACAGCG ACCTGCTTCG GCTGGAAGGT GATTCACGAA CTGTGCGGTT GCTAAGGGAG 
TTTCGTCGAG CCCTAGGACG GATGGTGGAG CGGAGCCTCC GCAGCGAGTT AGCTGCACTT 
CCTCTTAGTG TGCAGCAAGA ACACGGGCAG CTGCTTGCAC TGCTGGCAGA GTTGCTTCGA 
GGACCTTGTG GCAGCTTTGA TATGCGCAAG ACAGCTGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_006512305.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512368.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X11, mRNA.

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CloneID	OMu12947
Clone ID Related Accession (Same CDS sequence)	XM_006512303.2 , XM_006512303.1 , XM_030244640.1 , XM_030244641.1 , XM_006512304.1 , XM_006512305.3 , NM_001252682.1 , XM_006512305.4
Accession Version	XM_006512305.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512305.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCT TGGACTCATT CAGAAAAGAT CGGACCCAGC ACAGGCAGCG TCAATGCAAG 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCACT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCGCAGCCTA AAAACCTTCT CAACAGCGTG 
ATTGGAAGAG CCCTTGGCAT CTCACATGCA AAAGACAAGT TAGTCTATGT GCACACGAAT 
GGACCGAAGA AAAAGAAAGT CACCCTGCAC ATAAAGTGGC CCAAGAGCGT GGAGGTGGAA 
GGCTATGGCA GCAAGAAGAT TGATGCTGAG CGTCAGGCTG CAGCAGCTGC CTGCCAACTC 
TTCAAGGGCT GGGGTCTGCT GGGACCACGG AATGAGCTGT TTGATGCAGC TAAATACCGA 
GTGCTAGCTG ATCGTTTTGG GTCTCCAGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCTCCAACTT CCTGGCGGCA GCTGAATCCT GAGAACATTC GGCCAGGGGG TCCTGCAGGA 
CTATCCCGAT CCTTAGGCCG AGAGGAAGAG GAAGATGAGG AGGAAGAGCT AGAAGAGGGG 
ACCATTGATG TGACAGAGTT TCTGTCTATG ACCCAGCAAG ACTCCCACAA CCCACTCAGG 
GACTCAAGGG GGGGCTCCTT TGAAATGACA GATGATGACA GTGCTATCAG AGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTCCTGGCC AAAGTGATTC AGATTGCAAC CTCCTCCTCC 
ACAGCTAAGA ATCTCATGCA GTTCCATACT GTGGGTACCA AGACCAAGCT GGCTACACTC 
ACTCTGCTCT GGCCCTGTCC CATGACCTTT GTTGCCAAAG GGCGACGCAA AGCTGAGGCT 
GAGAATAAGG CAGCAGCCTT GGCTTGCAAG AAACTGAAGA GCCTTGGCCT TGTGGACAGG 
AACAATGAGC CGCTTACCCA TGCCATGTAC AACCTGGCCT CCCTGCGTGA GTTGGGTGAG 
ACCCAGCGCC GGCCATGTAC CATCCAGGTG CCTGAGCCCA TCCTTCGCAA GATAGAGGCC 
TTCCTGAGTC ATTACCCGGT GGACAGCTCA TGGATTTCCC CAGAACTCCG ACTGCAGAGT 
GATGACATCT TGCCCTTAGG CAAGGACTCA GGGCCCTTGA GTGACCCTAT CACAGGCAAG 
CCATACATGC CCCTGTCAGA AGCAGAGGAG GTGCGCTTAA GCCAGAGCCT GCTAGAGCTG 
TGGCGGAGGA GAGGGCCGAT CTGGCAGGAG GCCCCGCAGC TACCTGTAGA CCCTCATCGG 
GACACTATCC TCAGTGCCAT TGAACAACAC CCAGTGGTAG TCATCTCTGG GGACACAGGC 
TGTGGGAAGA CCACACGTAT CCCTCAGCTG CTATTGGAGC GCTATGTGAC TGAGGGTCGA 
GGTGCCCGCT GCAATGTGAT CATCACACAA CCTCGCCGGA TCTCAGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCATGAACT GGGCCCCTCC TTGCGCCGGA ATGTGGGCTT CCAGGTACGC 
TTGGAAAGTA AGCCCCCAGC CCGAGGTGGG GCGCTGCTCT TCTGCACTGT GGGTATCCTG 
CTTCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGTGTGA GCCATGTCAT TGTGGATGAG 
GTCCATGAGC GGGATGTGAA CACAGACTTC CTGCTGATTC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCAG CTCTTCGGCT GGTGCTCATG AGTGCTACAG GAGATAATGA GCGCTTTTCC 
CGATACTTTG GGGGCTGCCC TGTTATCAAG GTACCTGGCT TCATGTATCC CGTCAAGGAA 
CACTACCTGG AGGACATCTT GGCCAAGCTG GGTAAACATC AGTACCCACA CCGGCATCGG 
CACCATGAGT CGGAAGATGA ATGTGCACTT GATTTGGACC TTGTGACTGA CCTGGTTCTG 
CATATCGATG CCCGTGGGGA ACCAGGTGGG ATCCTGTGCT TTCTACCTGG CTGGCAAGAA 
ATCAAAGGAG TGCAACAACG GCTCCAGGAG GCCCTGGGCA TGCATGAAAG CAAGTACCTC 
ATCTTACCAG TGCACTCCAA TATCCCCATG ATGGACCAGA AGGCCATATT CCAACAGCCT 
CCACTTGGGG TACGCAAGAT TGTATTGGCC ACCAACATTG CTGAAACCTC CATCACAGTT 
AACGACATTG TACATGTCGT GGACAGCGGT CTGCACAAGG AGGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACTGTGTGG GTGTCGAGAG CAAATGTCAT TCAGCGCCGG 
GGCAGGGCAG GCCGCTGCCA GTCAGGTTTT GCCTACCACT TGTTCCCGAG GAGCCGGCTG 
GAGAAAATGG TTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCCA AAATCCATAT GCCTGAGAAG ACGGCAGTGG AGTTCCTCTC TAAGGCTGTG 
GACAGTCCAA ATATCAAGGC AGTGGATGAG GCCGTGATCC TGCTCCAGGA GATTGGGGTG 
CTGGACCAGC GGGAGTATCT GACCACCTTG GGACAGCGCC TGGCCCACAT CTCTACTGAC 
CCCCGACTGG CCAAGGCCAT AGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTGCTG 
GTGGTTGTTT CCTGCCTTAC CCGGGACCCC TTCAGCAGCA GTTTGCAGAA CCGGGCAGAA 
GTAGACAAGG TGAAGGCATT GCTGAGCCAT GACAGTGGCA GTGACCATTT GGCCTTCGTG 
CGGGCTGTGG CTGGCTGGGA GGAGGTACTG CGCTGGCAGG ACCGTACCTC CAGGGAAAAC 
TACCTGGAAG AAAACCTTCT GTATGCCCCC AGCTTGCGCT TCATCCACGG GCTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTAGTGGGGA AGCCCTCTGA CTGCACACTG 
CCCTCTGCTC AGTGCAATGA GTACAGCGAG GAAGAGGAGC TGGTGAAGGG TGTGCTGATG 
GCTGGCCTCT ACCCCAACCT CATCCAGGTG AGACAAGGTA AGGTTACTCG GCAAGGCAAG 
TTCAAACCCA ACAGTGTCAC TTACAGGACC AAATCTGGCA ACATCTTGCT GCATAAGTCA 
ACCATTAACA GGGAGGCTAC CCGGTTACGG AGCCGATGGC TGACATATTT CATGGCCGTC 
AAGTCCAATG GTAGCGTCTT TGTTCGAGAT TCCTCCCAGG TGCACCCACT AGCTGTGTTG 
CTCCTAACAG ATGGGGACGT GCACATCCGA GATGATGGGC GTCGGGCCAC CATCTCATTG 
AGTGACAGCG ACCTGCTTCG GCTGGAAGGT GATTCACGAA CTGTGCGGTT GCTAAGGGAG 
TTTCGTCGAG CCCTAGGACG GATGGTGGAG CGGAGCCTCC GCAGCGAGTT AGCTGCACTT 
CCTCTTAGTG TGCAGCAAGA ACACGGGCAG CTGCTTGCAC TGCTGGCAGA GTTGCTTCGA 
GGACCTTGTG GCAGCTTTGA TATGCGCAAG ACAGCTGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512368.1
CDS	394..3978
Translation	MFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_006512305.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512305.4

ORF Insert Sequence:

ATGTTCAGCT TGGACTCATT CAGAAAAGAT CGGACCCAGC ACAGGCAGCG TCAATGCAAG 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCACT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCGCAGCCTA AAAACCTTCT CAACAGCGTG 
ATTGGAAGAG CCCTTGGCAT CTCACATGCA AAAGACAAGT TAGTCTATGT GCACACGAAT 
GGACCGAAGA AAAAGAAAGT CACCCTGCAC ATAAAGTGGC CCAAGAGCGT GGAGGTGGAA 
GGCTATGGCA GCAAGAAGAT TGATGCTGAG CGTCAGGCTG CAGCAGCTGC CTGCCAACTC 
TTCAAGGGCT GGGGTCTGCT GGGACCACGG AATGAGCTGT TTGATGCAGC TAAATACCGA 
GTGCTAGCTG ATCGTTTTGG GTCTCCAGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCTCCAACTT CCTGGCGGCA GCTGAATCCT GAGAACATTC GGCCAGGGGG TCCTGCAGGA 
CTATCCCGAT CCTTAGGCCG AGAGGAAGAG GAAGATGAGG AGGAAGAGCT AGAAGAGGGG 
ACCATTGATG TGACAGAGTT TCTGTCTATG ACCCAGCAAG ACTCCCACAA CCCACTCAGG 
GACTCAAGGG GGGGCTCCTT TGAAATGACA GATGATGACA GTGCTATCAG AGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTCCTGGCC AAAGTGATTC AGATTGCAAC CTCCTCCTCC 
ACAGCTAAGA ATCTCATGCA GTTCCATACT GTGGGTACCA AGACCAAGCT GGCTACACTC 
ACTCTGCTCT GGCCCTGTCC CATGACCTTT GTTGCCAAAG GGCGACGCAA AGCTGAGGCT 
GAGAATAAGG CAGCAGCCTT GGCTTGCAAG AAACTGAAGA GCCTTGGCCT TGTGGACAGG 
AACAATGAGC CGCTTACCCA TGCCATGTAC AACCTGGCCT CCCTGCGTGA GTTGGGTGAG 
ACCCAGCGCC GGCCATGTAC CATCCAGGTG CCTGAGCCCA TCCTTCGCAA GATAGAGGCC 
TTCCTGAGTC ATTACCCGGT GGACAGCTCA TGGATTTCCC CAGAACTCCG ACTGCAGAGT 
GATGACATCT TGCCCTTAGG CAAGGACTCA GGGCCCTTGA GTGACCCTAT CACAGGCAAG 
CCATACATGC CCCTGTCAGA AGCAGAGGAG GTGCGCTTAA GCCAGAGCCT GCTAGAGCTG 
TGGCGGAGGA GAGGGCCGAT CTGGCAGGAG GCCCCGCAGC TACCTGTAGA CCCTCATCGG 
GACACTATCC TCAGTGCCAT TGAACAACAC CCAGTGGTAG TCATCTCTGG GGACACAGGC 
TGTGGGAAGA CCACACGTAT CCCTCAGCTG CTATTGGAGC GCTATGTGAC TGAGGGTCGA 
GGTGCCCGCT GCAATGTGAT CATCACACAA CCTCGCCGGA TCTCAGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCATGAACT GGGCCCCTCC TTGCGCCGGA ATGTGGGCTT CCAGGTACGC 
TTGGAAAGTA AGCCCCCAGC CCGAGGTGGG GCGCTGCTCT TCTGCACTGT GGGTATCCTG 
CTTCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGTGTGA GCCATGTCAT TGTGGATGAG 
GTCCATGAGC GGGATGTGAA CACAGACTTC CTGCTGATTC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCAG CTCTTCGGCT GGTGCTCATG AGTGCTACAG GAGATAATGA GCGCTTTTCC 
CGATACTTTG GGGGCTGCCC TGTTATCAAG GTACCTGGCT TCATGTATCC CGTCAAGGAA 
CACTACCTGG AGGACATCTT GGCCAAGCTG GGTAAACATC AGTACCCACA CCGGCATCGG 
CACCATGAGT CGGAAGATGA ATGTGCACTT GATTTGGACC TTGTGACTGA CCTGGTTCTG 
CATATCGATG CCCGTGGGGA ACCAGGTGGG ATCCTGTGCT TTCTACCTGG CTGGCAAGAA 
ATCAAAGGAG TGCAACAACG GCTCCAGGAG GCCCTGGGCA TGCATGAAAG CAAGTACCTC 
ATCTTACCAG TGCACTCCAA TATCCCCATG ATGGACCAGA AGGCCATATT CCAACAGCCT 
CCACTTGGGG TACGCAAGAT TGTATTGGCC ACCAACATTG CTGAAACCTC CATCACAGTT 
AACGACATTG TACATGTCGT GGACAGCGGT CTGCACAAGG AGGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACTGTGTGG GTGTCGAGAG CAAATGTCAT TCAGCGCCGG 
GGCAGGGCAG GCCGCTGCCA GTCAGGTTTT GCCTACCACT TGTTCCCGAG GAGCCGGCTG 
GAGAAAATGG TTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCCA AAATCCATAT GCCTGAGAAG ACGGCAGTGG AGTTCCTCTC TAAGGCTGTG 
GACAGTCCAA ATATCAAGGC AGTGGATGAG GCCGTGATCC TGCTCCAGGA GATTGGGGTG 
CTGGACCAGC GGGAGTATCT GACCACCTTG GGACAGCGCC TGGCCCACAT CTCTACTGAC 
CCCCGACTGG CCAAGGCCAT AGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTGCTG 
GTGGTTGTTT CCTGCCTTAC CCGGGACCCC TTCAGCAGCA GTTTGCAGAA CCGGGCAGAA 
GTAGACAAGG TGAAGGCATT GCTGAGCCAT GACAGTGGCA GTGACCATTT GGCCTTCGTG 
CGGGCTGTGG CTGGCTGGGA GGAGGTACTG CGCTGGCAGG ACCGTACCTC CAGGGAAAAC 
TACCTGGAAG AAAACCTTCT GTATGCCCCC AGCTTGCGCT TCATCCACGG GCTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTAGTGGGGA AGCCCTCTGA CTGCACACTG 
CCCTCTGCTC AGTGCAATGA GTACAGCGAG GAAGAGGAGC TGGTGAAGGG TGTGCTGATG 
GCTGGCCTCT ACCCCAACCT CATCCAGGTG AGACAAGGTA AGGTTACTCG GCAAGGCAAG 
TTCAAACCCA ACAGTGTCAC TTACAGGACC AAATCTGGCA ACATCTTGCT GCATAAGTCA 
ACCATTAACA GGGAGGCTAC CCGGTTACGG AGCCGATGGC TGACATATTT CATGGCCGTC 
AAGTCCAATG GTAGCGTCTT TGTTCGAGAT TCCTCCCAGG TGCACCCACT AGCTGTGTTG 
CTCCTAACAG ATGGGGACGT GCACATCCGA GATGATGGGC GTCGGGCCAC CATCTCATTG 
AGTGACAGCG ACCTGCTTCG GCTGGAAGGT GATTCACGAA CTGTGCGGTT GCTAAGGGAG 
TTTCGTCGAG CCCTAGGACG GATGGTGGAG CGGAGCCTCC GCAGCGAGTT AGCTGCACTT 
CCTCTTAGTG TGCAGCAAGA ACACGGGCAG CTGCTTGCAC TGCTGGCAGA GTTGCTTCGA 
GGACCTTGTG GCAGCTTTGA TATGCGCAAG ACAGCTGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_006512301.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512364.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X6, mRNA.

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CloneID	OMu13452
Clone ID Related Accession (Same CDS sequence)	XM_017313613.1 , XM_006512301.3 , XM_006512301.4 , XM_011243029.2 , XM_006512300.1 , XM_011243029.3 , XM_006512300.2 , XM_017313614.1 , XM_006512299.2 , XM_006512298.4 , NM_133347.2 , XM_006512299.1 , XM_006512298.3
Accession Version	XM_006512301.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512301.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512364.1
CDS	260..3913
Translation	MVTPVCNSSTWQPKDSSFLSWPEMFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_006512301.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512301.4

ORF Insert Sequence:

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_011243029.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241331.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X3, mRNA.

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CloneID	OMu13452
Clone ID Related Accession (Same CDS sequence)	XM_017313613.1 , XM_006512301.3 , XM_006512301.4 , XM_011243029.2 , XM_006512300.1 , XM_011243029.3 , XM_006512300.2 , XM_017313614.1 , XM_006512299.2 , XM_006512298.4 , NM_133347.2 , XM_006512299.1 , XM_006512298.3
Accession Version	XM_011243029.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011243029.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241331.1
CDS	379..4032
Translation	MVTPVCNSSTWQPKDSSFLSWPEMFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_011243029.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243029.3

ORF Insert Sequence:

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_006512300.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512363.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X4, mRNA.

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CloneID	OMu13452
Clone ID Related Accession (Same CDS sequence)	XM_017313613.1 , XM_006512301.3 , XM_006512301.4 , XM_011243029.2 , XM_006512300.1 , XM_011243029.3 , XM_006512300.2 , XM_017313614.1 , XM_006512299.2 , XM_006512298.4 , NM_133347.2 , XM_006512299.1 , XM_006512298.3
Accession Version	XM_006512300.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512300.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512363.1
CDS	680..4333
Translation	MVTPVCNSSTWQPKDSSFLSWPEMFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_006512300.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512300.2

ORF Insert Sequence:

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_006512299.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512362.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu13452
Clone ID Related Accession (Same CDS sequence)	XM_017313613.1 , XM_006512301.3 , XM_006512301.4 , XM_011243029.2 , XM_006512300.1 , XM_011243029.3 , XM_006512300.2 , XM_017313614.1 , XM_006512299.2 , XM_006512298.4 , NM_133347.2 , XM_006512299.1 , XM_006512298.3
Accession Version	XM_006512299.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512299.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512362.1
CDS	841..4494
Translation	MVTPVCNSSTWQPKDSSFLSWPEMFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_006512299.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512299.2

ORF Insert Sequence:

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_006512298.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512361.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X2, mRNA.

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CloneID	OMu13452
Clone ID Related Accession (Same CDS sequence)	XM_017313613.1 , XM_006512301.3 , XM_006512301.4 , XM_011243029.2 , XM_006512300.1 , XM_011243029.3 , XM_006512300.2 , XM_017313614.1 , XM_006512299.2 , XM_006512298.4 , NM_133347.2 , XM_006512299.1 , XM_006512298.3
Accession Version	XM_006512298.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512298.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512361.1
CDS	262..3915
Translation	MVTPVCNSSTWQPKDSSFLSWPEMFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_006512298.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512298.4

ORF Insert Sequence:

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_030244646.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100506.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X17, mRNA.

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CloneID	OMu43563
Clone ID Related Accession (Same CDS sequence)	XM_006512310.3 , XM_030244646.1 , XM_030244645.1 , XM_017313615.1 , XM_006512310.4 , XM_017313616.1 , XM_030244642.1 , XM_030244643.1 , XM_030244644.1 , XM_030244647.1
Accession Version	XM_030244646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100506.1
CDS	963..4523
Translation	MQASPTPSSTHVCQPCPWRDHHSRLNVNISNMAASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_030244646.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244646.1

ORF Insert Sequence:

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_030244645.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100505.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OMu43563
Clone ID Related Accession (Same CDS sequence)	XM_006512310.3 , XM_030244646.1 , XM_030244645.1 , XM_017313615.1 , XM_006512310.4 , XM_017313616.1 , XM_030244642.1 , XM_030244643.1 , XM_030244644.1 , XM_030244647.1
Accession Version	XM_030244645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100505.1
CDS	548..4108
Translation	MQASPTPSSTHVCQPCPWRDHHSRLNVNISNMAASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_030244645.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244645.1

ORF Insert Sequence:

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_006512310.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512373.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X18, mRNA.

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CloneID	OMu43563
Clone ID Related Accession (Same CDS sequence)	XM_006512310.3 , XM_030244646.1 , XM_030244645.1 , XM_017313615.1 , XM_006512310.4 , XM_017313616.1 , XM_030244642.1 , XM_030244643.1 , XM_030244644.1 , XM_030244647.1
Accession Version	XM_006512310.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512310.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512373.1
CDS	384..3944
Translation	MQASPTPSSTHVCQPCPWRDHHSRLNVNISNMAASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_006512310.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512310.4

ORF Insert Sequence:

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_030244642.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100502.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X13, mRNA.

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CloneID	OMu43563
Clone ID Related Accession (Same CDS sequence)	XM_006512310.3 , XM_030244646.1 , XM_030244645.1 , XM_017313615.1 , XM_006512310.4 , XM_017313616.1 , XM_030244642.1 , XM_030244643.1 , XM_030244644.1 , XM_030244647.1
Accession Version	XM_030244642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100502.1
CDS	453..4013
Translation	MQASPTPSSTHVCQPCPWRDHHSRLNVNISNMAASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_030244642.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244642.1

ORF Insert Sequence:

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_030244643.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100503.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X14, mRNA.

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CloneID	OMu43563
Clone ID Related Accession (Same CDS sequence)	XM_006512310.3 , XM_030244646.1 , XM_030244645.1 , XM_017313615.1 , XM_006512310.4 , XM_017313616.1 , XM_030244642.1 , XM_030244643.1 , XM_030244644.1 , XM_030244647.1
Accession Version	XM_030244643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100503.1
CDS	867..4427
Translation	MQASPTPSSTHVCQPCPWRDHHSRLNVNISNMAASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_030244643.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244643.1

ORF Insert Sequence:

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_030244644.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100504.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X15, mRNA.

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CloneID	OMu43563
Clone ID Related Accession (Same CDS sequence)	XM_006512310.3 , XM_030244646.1 , XM_030244645.1 , XM_017313615.1 , XM_006512310.4 , XM_017313616.1 , XM_030244642.1 , XM_030244643.1 , XM_030244644.1 , XM_030244647.1
Accession Version	XM_030244644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100504.1
CDS	447..4007
Translation	MQASPTPSSTHVCQPCPWRDHHSRLNVNISNMAASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_030244644.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244644.1

ORF Insert Sequence:

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_030244647.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100507.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OMu43563
Clone ID Related Accession (Same CDS sequence)	XM_006512310.3 , XM_030244646.1 , XM_030244645.1 , XM_017313615.1 , XM_006512310.4 , XM_017313616.1 , XM_030244642.1 , XM_030244643.1 , XM_030244644.1 , XM_030244647.1
Accession Version	XM_030244647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100507.1
CDS	802..4362
Translation	MQASPTPSSTHVCQPCPWRDHHSRLNVNISNMAASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_030244647.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244647.1

ORF Insert Sequence:

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( NM_001252682.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239611.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant 1, mRNA.

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CloneID	OMu12947
Clone ID Related Accession (Same CDS sequence)	XM_006512303.2 , XM_006512303.1 , XM_030244640.1 , XM_030244641.1 , XM_006512304.1 , XM_006512305.3 , NM_001252682.1 , XM_006512305.4
Accession Version	NM_001252682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC159372.1, AK159601.1, CF724787.1 and BY540912.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the shortest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK146326.1, AK159601.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTTCAGCT TGGACTCATT CAGAAAAGAT CGGACCCAGC ACAGGCAGCG TCAATGCAAG 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCACT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCGCAGCCTA AAAACCTTCT CAACAGCGTG 
ATTGGAAGAG CCCTTGGCAT CTCACATGCA AAAGACAAGT TAGTCTATGT GCACACGAAT 
GGACCGAAGA AAAAGAAAGT CACCCTGCAC ATAAAGTGGC CCAAGAGCGT GGAGGTGGAA 
GGCTATGGCA GCAAGAAGAT TGATGCTGAG CGTCAGGCTG CAGCAGCTGC CTGCCAACTC 
TTCAAGGGCT GGGGTCTGCT GGGACCACGG AATGAGCTGT TTGATGCAGC TAAATACCGA 
GTGCTAGCTG ATCGTTTTGG GTCTCCAGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCTCCAACTT CCTGGCGGCA GCTGAATCCT GAGAACATTC GGCCAGGGGG TCCTGCAGGA 
CTATCCCGAT CCTTAGGCCG AGAGGAAGAG GAAGATGAGG AGGAAGAGCT AGAAGAGGGG 
ACCATTGATG TGACAGAGTT TCTGTCTATG ACCCAGCAAG ACTCCCACAA CCCACTCAGG 
GACTCAAGGG GGGGCTCCTT TGAAATGACA GATGATGACA GTGCTATCAG AGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTCCTGGCC AAAGTGATTC AGATTGCAAC CTCCTCCTCC 
ACAGCTAAGA ATCTCATGCA GTTCCATACT GTGGGTACCA AGACCAAGCT GGCTACACTC 
ACTCTGCTCT GGCCCTGTCC CATGACCTTT GTTGCCAAAG GGCGACGCAA AGCTGAGGCT 
GAGAATAAGG CAGCAGCCTT GGCTTGCAAG AAACTGAAGA GCCTTGGCCT TGTGGACAGG 
AACAATGAGC CGCTTACCCA TGCCATGTAC AACCTGGCCT CCCTGCGTGA GTTGGGTGAG 
ACCCAGCGCC GGCCATGTAC CATCCAGGTG CCTGAGCCCA TCCTTCGCAA GATAGAGGCC 
TTCCTGAGTC ATTACCCGGT GGACAGCTCA TGGATTTCCC CAGAACTCCG ACTGCAGAGT 
GATGACATCT TGCCCTTAGG CAAGGACTCA GGGCCCTTGA GTGACCCTAT CACAGGCAAG 
CCATACATGC CCCTGTCAGA AGCAGAGGAG GTGCGCTTAA GCCAGAGCCT GCTAGAGCTG 
TGGCGGAGGA GAGGGCCGAT CTGGCAGGAG GCCCCGCAGC TACCTGTAGA CCCTCATCGG 
GACACTATCC TCAGTGCCAT TGAACAACAC CCAGTGGTAG TCATCTCTGG GGACACAGGC 
TGTGGGAAGA CCACACGTAT CCCTCAGCTG CTATTGGAGC GCTATGTGAC TGAGGGTCGA 
GGTGCCCGCT GCAATGTGAT CATCACACAA CCTCGCCGGA TCTCAGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCATGAACT GGGCCCCTCC TTGCGCCGGA ATGTGGGCTT CCAGGTACGC 
TTGGAAAGTA AGCCCCCAGC CCGAGGTGGG GCGCTGCTCT TCTGCACTGT GGGTATCCTG 
CTTCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGTGTGA GCCATGTCAT TGTGGATGAG 
GTCCATGAGC GGGATGTGAA CACAGACTTC CTGCTGATTC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCAG CTCTTCGGCT GGTGCTCATG AGTGCTACAG GAGATAATGA GCGCTTTTCC 
CGATACTTTG GGGGCTGCCC TGTTATCAAG GTACCTGGCT TCATGTATCC CGTCAAGGAA 
CACTACCTGG AGGACATCTT GGCCAAGCTG GGTAAACATC AGTACCCACA CCGGCATCGG 
CACCATGAGT CGGAAGATGA ATGTGCACTT GATTTGGACC TTGTGACTGA CCTGGTTCTG 
CATATCGATG CCCGTGGGGA ACCAGGTGGG ATCCTGTGCT TTCTACCTGG CTGGCAAGAA 
ATCAAAGGAG TGCAACAACG GCTCCAGGAG GCCCTGGGCA TGCATGAAAG CAAGTACCTC 
ATCTTACCAG TGCACTCCAA TATCCCCATG ATGGACCAGA AGGCCATATT CCAACAGCCT 
CCACTTGGGG TACGCAAGAT TGTATTGGCC ACCAACATTG CTGAAACCTC CATCACAGTT 
AACGACATTG TACATGTCGT GGACAGCGGT CTGCACAAGG AGGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACTGTGTGG GTGTCGAGAG CAAATGTCAT TCAGCGCCGG 
GGCAGGGCAG GCCGCTGCCA GTCAGGTTTT GCCTACCACT TGTTCCCGAG GAGCCGGCTG 
GAGAAAATGG TTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCCA AAATCCATAT GCCTGAGAAG ACGGCAGTGG AGTTCCTCTC TAAGGCTGTG 
GACAGTCCAA ATATCAAGGC AGTGGATGAG GCCGTGATCC TGCTCCAGGA GATTGGGGTG 
CTGGACCAGC GGGAGTATCT GACCACCTTG GGACAGCGCC TGGCCCACAT CTCTACTGAC 
CCCCGACTGG CCAAGGCCAT AGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTGCTG 
GTGGTTGTTT CCTGCCTTAC CCGGGACCCC TTCAGCAGCA GTTTGCAGAA CCGGGCAGAA 
GTAGACAAGG TGAAGGCATT GCTGAGCCAT GACAGTGGCA GTGACCATTT GGCCTTCGTG 
CGGGCTGTGG CTGGCTGGGA GGAGGTACTG CGCTGGCAGG ACCGTACCTC CAGGGAAAAC 
TACCTGGAAG AAAACCTTCT GTATGCCCCC AGCTTGCGCT TCATCCACGG GCTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTAGTGGGGA AGCCCTCTGA CTGCACACTG 
CCCTCTGCTC AGTGCAATGA GTACAGCGAG GAAGAGGAGC TGGTGAAGGG TGTGCTGATG 
GCTGGCCTCT ACCCCAACCT CATCCAGGTG AGACAAGGTA AGGTTACTCG GCAAGGCAAG 
TTCAAACCCA ACAGTGTCAC TTACAGGACC AAATCTGGCA ACATCTTGCT GCATAAGTCA 
ACCATTAACA GGGAGGCTAC CCGGTTACGG AGCCGATGGC TGACATATTT CATGGCCGTC 
AAGTCCAATG GTAGCGTCTT TGTTCGAGAT TCCTCCCAGG TGCACCCACT AGCTGTGTTG 
CTCCTAACAG ATGGGGACGT GCACATCCGA GATGATGGGC GTCGGGCCAC CATCTCATTG 
AGTGACAGCG ACCTGCTTCG GCTGGAAGGT GATTCACGAA CTGTGCGGTT GCTAAGGGAG 
TTTCGTCGAG CCCTAGGACG GATGGTGGAG CGGAGCCTCC GCAGCGAGTT AGCTGCACTT 
CCTCTTAGTG TGCAGCAAGA ACACGGGCAG CTGCTTGCAC TGCTGGCAGA GTTGCTTCGA 
GGACCTTGTG GCAGCTTTGA TATGCGCAAG ACAGCTGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239611.1
CDS	570..4154
Translation	MFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	NM_001252682.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252682.1

ORF Insert Sequence:

ATGTTCAGCT TGGACTCATT CAGAAAAGAT CGGACCCAGC ACAGGCAGCG TCAATGCAAG 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCACT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCGCAGCCTA AAAACCTTCT CAACAGCGTG 
ATTGGAAGAG CCCTTGGCAT CTCACATGCA AAAGACAAGT TAGTCTATGT GCACACGAAT 
GGACCGAAGA AAAAGAAAGT CACCCTGCAC ATAAAGTGGC CCAAGAGCGT GGAGGTGGAA 
GGCTATGGCA GCAAGAAGAT TGATGCTGAG CGTCAGGCTG CAGCAGCTGC CTGCCAACTC 
TTCAAGGGCT GGGGTCTGCT GGGACCACGG AATGAGCTGT TTGATGCAGC TAAATACCGA 
GTGCTAGCTG ATCGTTTTGG GTCTCCAGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCTCCAACTT CCTGGCGGCA GCTGAATCCT GAGAACATTC GGCCAGGGGG TCCTGCAGGA 
CTATCCCGAT CCTTAGGCCG AGAGGAAGAG GAAGATGAGG AGGAAGAGCT AGAAGAGGGG 
ACCATTGATG TGACAGAGTT TCTGTCTATG ACCCAGCAAG ACTCCCACAA CCCACTCAGG 
GACTCAAGGG GGGGCTCCTT TGAAATGACA GATGATGACA GTGCTATCAG AGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTCCTGGCC AAAGTGATTC AGATTGCAAC CTCCTCCTCC 
ACAGCTAAGA ATCTCATGCA GTTCCATACT GTGGGTACCA AGACCAAGCT GGCTACACTC 
ACTCTGCTCT GGCCCTGTCC CATGACCTTT GTTGCCAAAG GGCGACGCAA AGCTGAGGCT 
GAGAATAAGG CAGCAGCCTT GGCTTGCAAG AAACTGAAGA GCCTTGGCCT TGTGGACAGG 
AACAATGAGC CGCTTACCCA TGCCATGTAC AACCTGGCCT CCCTGCGTGA GTTGGGTGAG 
ACCCAGCGCC GGCCATGTAC CATCCAGGTG CCTGAGCCCA TCCTTCGCAA GATAGAGGCC 
TTCCTGAGTC ATTACCCGGT GGACAGCTCA TGGATTTCCC CAGAACTCCG ACTGCAGAGT 
GATGACATCT TGCCCTTAGG CAAGGACTCA GGGCCCTTGA GTGACCCTAT CACAGGCAAG 
CCATACATGC CCCTGTCAGA AGCAGAGGAG GTGCGCTTAA GCCAGAGCCT GCTAGAGCTG 
TGGCGGAGGA GAGGGCCGAT CTGGCAGGAG GCCCCGCAGC TACCTGTAGA CCCTCATCGG 
GACACTATCC TCAGTGCCAT TGAACAACAC CCAGTGGTAG TCATCTCTGG GGACACAGGC 
TGTGGGAAGA CCACACGTAT CCCTCAGCTG CTATTGGAGC GCTATGTGAC TGAGGGTCGA 
GGTGCCCGCT GCAATGTGAT CATCACACAA CCTCGCCGGA TCTCAGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCATGAACT GGGCCCCTCC TTGCGCCGGA ATGTGGGCTT CCAGGTACGC 
TTGGAAAGTA AGCCCCCAGC CCGAGGTGGG GCGCTGCTCT TCTGCACTGT GGGTATCCTG 
CTTCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGTGTGA GCCATGTCAT TGTGGATGAG 
GTCCATGAGC GGGATGTGAA CACAGACTTC CTGCTGATTC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCAG CTCTTCGGCT GGTGCTCATG AGTGCTACAG GAGATAATGA GCGCTTTTCC 
CGATACTTTG GGGGCTGCCC TGTTATCAAG GTACCTGGCT TCATGTATCC CGTCAAGGAA 
CACTACCTGG AGGACATCTT GGCCAAGCTG GGTAAACATC AGTACCCACA CCGGCATCGG 
CACCATGAGT CGGAAGATGA ATGTGCACTT GATTTGGACC TTGTGACTGA CCTGGTTCTG 
CATATCGATG CCCGTGGGGA ACCAGGTGGG ATCCTGTGCT TTCTACCTGG CTGGCAAGAA 
ATCAAAGGAG TGCAACAACG GCTCCAGGAG GCCCTGGGCA TGCATGAAAG CAAGTACCTC 
ATCTTACCAG TGCACTCCAA TATCCCCATG ATGGACCAGA AGGCCATATT CCAACAGCCT 
CCACTTGGGG TACGCAAGAT TGTATTGGCC ACCAACATTG CTGAAACCTC CATCACAGTT 
AACGACATTG TACATGTCGT GGACAGCGGT CTGCACAAGG AGGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACTGTGTGG GTGTCGAGAG CAAATGTCAT TCAGCGCCGG 
GGCAGGGCAG GCCGCTGCCA GTCAGGTTTT GCCTACCACT TGTTCCCGAG GAGCCGGCTG 
GAGAAAATGG TTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCCA AAATCCATAT GCCTGAGAAG ACGGCAGTGG AGTTCCTCTC TAAGGCTGTG 
GACAGTCCAA ATATCAAGGC AGTGGATGAG GCCGTGATCC TGCTCCAGGA GATTGGGGTG 
CTGGACCAGC GGGAGTATCT GACCACCTTG GGACAGCGCC TGGCCCACAT CTCTACTGAC 
CCCCGACTGG CCAAGGCCAT AGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTGCTG 
GTGGTTGTTT CCTGCCTTAC CCGGGACCCC TTCAGCAGCA GTTTGCAGAA CCGGGCAGAA 
GTAGACAAGG TGAAGGCATT GCTGAGCCAT GACAGTGGCA GTGACCATTT GGCCTTCGTG 
CGGGCTGTGG CTGGCTGGGA GGAGGTACTG CGCTGGCAGG ACCGTACCTC CAGGGAAAAC 
TACCTGGAAG AAAACCTTCT GTATGCCCCC AGCTTGCGCT TCATCCACGG GCTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTAGTGGGGA AGCCCTCTGA CTGCACACTG 
CCCTCTGCTC AGTGCAATGA GTACAGCGAG GAAGAGGAGC TGGTGAAGGG TGTGCTGATG 
GCTGGCCTCT ACCCCAACCT CATCCAGGTG AGACAAGGTA AGGTTACTCG GCAAGGCAAG 
TTCAAACCCA ACAGTGTCAC TTACAGGACC AAATCTGGCA ACATCTTGCT GCATAAGTCA 
ACCATTAACA GGGAGGCTAC CCGGTTACGG AGCCGATGGC TGACATATTT CATGGCCGTC 
AAGTCCAATG GTAGCGTCTT TGTTCGAGAT TCCTCCCAGG TGCACCCACT AGCTGTGTTG 
CTCCTAACAG ATGGGGACGT GCACATCCGA GATGATGGGC GTCGGGCCAC CATCTCATTG 
AGTGACAGCG ACCTGCTTCG GCTGGAAGGT GATTCACGAA CTGTGCGGTT GCTAAGGGAG 
TTTCGTCGAG CCCTAGGACG GATGGTGGAG CGGAGCCTCC GCAGCGAGTT AGCTGCACTT 
CCTCTTAGTG TGCAGCAAGA ACACGGGCAG CTGCTTGCAC TGCTGGCAGA GTTGCTTCGA 
GGACCTTGTG GCAGCTTTGA TATGCGCAAG ACAGCTGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( NM_133347.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_579925.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant 2, mRNA.

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CloneID	OMu13452
Clone ID Related Accession (Same CDS sequence)	XM_017313613.1 , XM_006512301.3 , XM_006512301.4 , XM_011243029.2 , XM_006512300.1 , XM_011243029.3 , XM_006512300.2 , XM_017313614.1 , XM_006512299.2 , XM_006512298.4 , NM_133347.2 , XM_006512299.1 , XM_006512298.3
Accession Version	NM_133347.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB047557.1 and BY540912.1. On Nov 26, 2011 this sequence version replaced NM_133347.1. Transcript Variant: This variant (2) uses an alternate splice site and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is longer and has a distinct N-terminus, compared to isoform 1. ##Evidence-Data-START## CDS exon combination :: SRR1660819.67956.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_579925.1
CDS	177..3830
Translation	MVTPVCNSSTWQPKDSSFLSWPEMFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	NM_133347.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133347.2

ORF Insert Sequence:

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_006512303.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512366.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X8, mRNA.

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CloneID	OMu12947
Clone ID Related Accession (Same CDS sequence)	XM_006512303.2 , XM_006512303.1 , XM_030244640.1 , XM_030244641.1 , XM_006512304.1 , XM_006512305.3 , NM_001252682.1 , XM_006512305.4
Accession Version	XM_006512303.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	putative ATP-dependent RNA helicase DHX30 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCT TGGACTCATT CAGAAAAGAT CGGACCCAGC ACAGGCAGCG TCAATGCAAG 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCACT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCGCAGCCTA AAAACCTTCT CAACAGCGTG 
ATTGGAAGAG CCCTTGGCAT CTCACATGCA AAAGACAAGT TAGTCTATGT GCACACGAAT 
GGACCGAAGA AAAAGAAAGT CACCCTGCAC ATAAAGTGGC CCAAGAGCGT GGAGGTGGAA 
GGCTATGGCA GCAAGAAGAT TGATGCTGAG CGTCAGGCTG CAGCAGCTGC CTGCCAACTC 
TTCAAGGGCT GGGGTCTGCT GGGACCACGG AATGAGCTGT TTGATGCAGC TAAATACCGA 
GTGCTAGCTG ATCGTTTTGG GTCTCCAGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCTCCAACTT CCTGGCGGCA GCTGAATCCT GAGAACATTC GGCCAGGGGG TCCTGCAGGA 
CTATCCCGAT CCTTAGGCCG AGAGGAAGAG GAAGATGAGG AGGAAGAGCT AGAAGAGGGG 
ACCATTGATG TGACAGAGTT TCTGTCTATG ACCCAGCAAG ACTCCCACAA CCCACTCAGG 
GACTCAAGGG GGGGCTCCTT TGAAATGACA GATGATGACA GTGCTATCAG AGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTCCTGGCC AAAGTGATTC AGATTGCAAC CTCCTCCTCC 
ACAGCTAAGA ATCTCATGCA GTTCCATACT GTGGGTACCA AGACCAAGCT GGCTACACTC 
ACTCTGCTCT GGCCCTGTCC CATGACCTTT GTTGCCAAAG GGCGACGCAA AGCTGAGGCT 
GAGAATAAGG CAGCAGCCTT GGCTTGCAAG AAACTGAAGA GCCTTGGCCT TGTGGACAGG 
AACAATGAGC CGCTTACCCA TGCCATGTAC AACCTGGCCT CCCTGCGTGA GTTGGGTGAG 
ACCCAGCGCC GGCCATGTAC CATCCAGGTG CCTGAGCCCA TCCTTCGCAA GATAGAGGCC 
TTCCTGAGTC ATTACCCGGT GGACAGCTCA TGGATTTCCC CAGAACTCCG ACTGCAGAGT 
GATGACATCT TGCCCTTAGG CAAGGACTCA GGGCCCTTGA GTGACCCTAT CACAGGCAAG 
CCATACATGC CCCTGTCAGA AGCAGAGGAG GTGCGCTTAA GCCAGAGCCT GCTAGAGCTG 
TGGCGGAGGA GAGGGCCGAT CTGGCAGGAG GCCCCGCAGC TACCTGTAGA CCCTCATCGG 
GACACTATCC TCAGTGCCAT TGAACAACAC CCAGTGGTAG TCATCTCTGG GGACACAGGC 
TGTGGGAAGA CCACACGTAT CCCTCAGCTG CTATTGGAGC GCTATGTGAC TGAGGGTCGA 
GGTGCCCGCT GCAATGTGAT CATCACACAA CCTCGCCGGA TCTCAGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCATGAACT GGGCCCCTCC TTGCGCCGGA ATGTGGGCTT CCAGGTACGC 
TTGGAAAGTA AGCCCCCAGC CCGAGGTGGG GCGCTGCTCT TCTGCACTGT GGGTATCCTG 
CTTCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGTGTGA GCCATGTCAT TGTGGATGAG 
GTCCATGAGC GGGATGTGAA CACAGACTTC CTGCTGATTC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCAG CTCTTCGGCT GGTGCTCATG AGTGCTACAG GAGATAATGA GCGCTTTTCC 
CGATACTTTG GGGGCTGCCC TGTTATCAAG GTACCTGGCT TCATGTATCC CGTCAAGGAA 
CACTACCTGG AGGACATCTT GGCCAAGCTG GGTAAACATC AGTACCCACA CCGGCATCGG 
CACCATGAGT CGGAAGATGA ATGTGCACTT GATTTGGACC TTGTGACTGA CCTGGTTCTG 
CATATCGATG CCCGTGGGGA ACCAGGTGGG ATCCTGTGCT TTCTACCTGG CTGGCAAGAA 
ATCAAAGGAG TGCAACAACG GCTCCAGGAG GCCCTGGGCA TGCATGAAAG CAAGTACCTC 
ATCTTACCAG TGCACTCCAA TATCCCCATG ATGGACCAGA AGGCCATATT CCAACAGCCT 
CCACTTGGGG TACGCAAGAT TGTATTGGCC ACCAACATTG CTGAAACCTC CATCACAGTT 
AACGACATTG TACATGTCGT GGACAGCGGT CTGCACAAGG AGGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACTGTGTGG GTGTCGAGAG CAAATGTCAT TCAGCGCCGG 
GGCAGGGCAG GCCGCTGCCA GTCAGGTTTT GCCTACCACT TGTTCCCGAG GAGCCGGCTG 
GAGAAAATGG TTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCCA AAATCCATAT GCCTGAGAAG ACGGCAGTGG AGTTCCTCTC TAAGGCTGTG 
GACAGTCCAA ATATCAAGGC AGTGGATGAG GCCGTGATCC TGCTCCAGGA GATTGGGGTG 
CTGGACCAGC GGGAGTATCT GACCACCTTG GGACAGCGCC TGGCCCACAT CTCTACTGAC 
CCCCGACTGG CCAAGGCCAT AGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTGCTG 
GTGGTTGTTT CCTGCCTTAC CCGGGACCCC TTCAGCAGCA GTTTGCAGAA CCGGGCAGAA 
GTAGACAAGG TGAAGGCATT GCTGAGCCAT GACAGTGGCA GTGACCATTT GGCCTTCGTG 
CGGGCTGTGG CTGGCTGGGA GGAGGTACTG CGCTGGCAGG ACCGTACCTC CAGGGAAAAC 
TACCTGGAAG AAAACCTTCT GTATGCCCCC AGCTTGCGCT TCATCCACGG GCTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTAGTGGGGA AGCCCTCTGA CTGCACACTG 
CCCTCTGCTC AGTGCAATGA GTACAGCGAG GAAGAGGAGC TGGTGAAGGG TGTGCTGATG 
GCTGGCCTCT ACCCCAACCT CATCCAGGTG AGACAAGGTA AGGTTACTCG GCAAGGCAAG 
TTCAAACCCA ACAGTGTCAC TTACAGGACC AAATCTGGCA ACATCTTGCT GCATAAGTCA 
ACCATTAACA GGGAGGCTAC CCGGTTACGG AGCCGATGGC TGACATATTT CATGGCCGTC 
AAGTCCAATG GTAGCGTCTT TGTTCGAGAT TCCTCCCAGG TGCACCCACT AGCTGTGTTG 
CTCCTAACAG ATGGGGACGT GCACATCCGA GATGATGGGC GTCGGGCCAC CATCTCATTG 
AGTGACAGCG ACCTGCTTCG GCTGGAAGGT GATTCACGAA CTGTGCGGTT GCTAAGGGAG 
TTTCGTCGAG CCCTAGGACG GATGGTGGAG CGGAGCCTCC GCAGCGAGTT AGCTGCACTT 
CCTCTTAGTG TGCAGCAAGA ACACGGGCAG CTGCTTGCAC TGCTGGCAGA GTTGCTTCGA 
GGACCTTGTG GCAGCTTTGA TATGCGCAAG ACAGCTGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512366.1
CDS	975..4559
Translation	MFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_006512303.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512303.1

ORF Insert Sequence:

ATGTTCAGCT TGGACTCATT CAGAAAAGAT CGGACCCAGC ACAGGCAGCG TCAATGCAAG 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCACT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCGCAGCCTA AAAACCTTCT CAACAGCGTG 
ATTGGAAGAG CCCTTGGCAT CTCACATGCA AAAGACAAGT TAGTCTATGT GCACACGAAT 
GGACCGAAGA AAAAGAAAGT CACCCTGCAC ATAAAGTGGC CCAAGAGCGT GGAGGTGGAA 
GGCTATGGCA GCAAGAAGAT TGATGCTGAG CGTCAGGCTG CAGCAGCTGC CTGCCAACTC 
TTCAAGGGCT GGGGTCTGCT GGGACCACGG AATGAGCTGT TTGATGCAGC TAAATACCGA 
GTGCTAGCTG ATCGTTTTGG GTCTCCAGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCTCCAACTT CCTGGCGGCA GCTGAATCCT GAGAACATTC GGCCAGGGGG TCCTGCAGGA 
CTATCCCGAT CCTTAGGCCG AGAGGAAGAG GAAGATGAGG AGGAAGAGCT AGAAGAGGGG 
ACCATTGATG TGACAGAGTT TCTGTCTATG ACCCAGCAAG ACTCCCACAA CCCACTCAGG 
GACTCAAGGG GGGGCTCCTT TGAAATGACA GATGATGACA GTGCTATCAG AGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTCCTGGCC AAAGTGATTC AGATTGCAAC CTCCTCCTCC 
ACAGCTAAGA ATCTCATGCA GTTCCATACT GTGGGTACCA AGACCAAGCT GGCTACACTC 
ACTCTGCTCT GGCCCTGTCC CATGACCTTT GTTGCCAAAG GGCGACGCAA AGCTGAGGCT 
GAGAATAAGG CAGCAGCCTT GGCTTGCAAG AAACTGAAGA GCCTTGGCCT TGTGGACAGG 
AACAATGAGC CGCTTACCCA TGCCATGTAC AACCTGGCCT CCCTGCGTGA GTTGGGTGAG 
ACCCAGCGCC GGCCATGTAC CATCCAGGTG CCTGAGCCCA TCCTTCGCAA GATAGAGGCC 
TTCCTGAGTC ATTACCCGGT GGACAGCTCA TGGATTTCCC CAGAACTCCG ACTGCAGAGT 
GATGACATCT TGCCCTTAGG CAAGGACTCA GGGCCCTTGA GTGACCCTAT CACAGGCAAG 
CCATACATGC CCCTGTCAGA AGCAGAGGAG GTGCGCTTAA GCCAGAGCCT GCTAGAGCTG 
TGGCGGAGGA GAGGGCCGAT CTGGCAGGAG GCCCCGCAGC TACCTGTAGA CCCTCATCGG 
GACACTATCC TCAGTGCCAT TGAACAACAC CCAGTGGTAG TCATCTCTGG GGACACAGGC 
TGTGGGAAGA CCACACGTAT CCCTCAGCTG CTATTGGAGC GCTATGTGAC TGAGGGTCGA 
GGTGCCCGCT GCAATGTGAT CATCACACAA CCTCGCCGGA TCTCAGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCATGAACT GGGCCCCTCC TTGCGCCGGA ATGTGGGCTT CCAGGTACGC 
TTGGAAAGTA AGCCCCCAGC CCGAGGTGGG GCGCTGCTCT TCTGCACTGT GGGTATCCTG 
CTTCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGTGTGA GCCATGTCAT TGTGGATGAG 
GTCCATGAGC GGGATGTGAA CACAGACTTC CTGCTGATTC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCAG CTCTTCGGCT GGTGCTCATG AGTGCTACAG GAGATAATGA GCGCTTTTCC 
CGATACTTTG GGGGCTGCCC TGTTATCAAG GTACCTGGCT TCATGTATCC CGTCAAGGAA 
CACTACCTGG AGGACATCTT GGCCAAGCTG GGTAAACATC AGTACCCACA CCGGCATCGG 
CACCATGAGT CGGAAGATGA ATGTGCACTT GATTTGGACC TTGTGACTGA CCTGGTTCTG 
CATATCGATG CCCGTGGGGA ACCAGGTGGG ATCCTGTGCT TTCTACCTGG CTGGCAAGAA 
ATCAAAGGAG TGCAACAACG GCTCCAGGAG GCCCTGGGCA TGCATGAAAG CAAGTACCTC 
ATCTTACCAG TGCACTCCAA TATCCCCATG ATGGACCAGA AGGCCATATT CCAACAGCCT 
CCACTTGGGG TACGCAAGAT TGTATTGGCC ACCAACATTG CTGAAACCTC CATCACAGTT 
AACGACATTG TACATGTCGT GGACAGCGGT CTGCACAAGG AGGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACTGTGTGG GTGTCGAGAG CAAATGTCAT TCAGCGCCGG 
GGCAGGGCAG GCCGCTGCCA GTCAGGTTTT GCCTACCACT TGTTCCCGAG GAGCCGGCTG 
GAGAAAATGG TTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCCA AAATCCATAT GCCTGAGAAG ACGGCAGTGG AGTTCCTCTC TAAGGCTGTG 
GACAGTCCAA ATATCAAGGC AGTGGATGAG GCCGTGATCC TGCTCCAGGA GATTGGGGTG 
CTGGACCAGC GGGAGTATCT GACCACCTTG GGACAGCGCC TGGCCCACAT CTCTACTGAC 
CCCCGACTGG CCAAGGCCAT AGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTGCTG 
GTGGTTGTTT CCTGCCTTAC CCGGGACCCC TTCAGCAGCA GTTTGCAGAA CCGGGCAGAA 
GTAGACAAGG TGAAGGCATT GCTGAGCCAT GACAGTGGCA GTGACCATTT GGCCTTCGTG 
CGGGCTGTGG CTGGCTGGGA GGAGGTACTG CGCTGGCAGG ACCGTACCTC CAGGGAAAAC 
TACCTGGAAG AAAACCTTCT GTATGCCCCC AGCTTGCGCT TCATCCACGG GCTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTAGTGGGGA AGCCCTCTGA CTGCACACTG 
CCCTCTGCTC AGTGCAATGA GTACAGCGAG GAAGAGGAGC TGGTGAAGGG TGTGCTGATG 
GCTGGCCTCT ACCCCAACCT CATCCAGGTG AGACAAGGTA AGGTTACTCG GCAAGGCAAG 
TTCAAACCCA ACAGTGTCAC TTACAGGACC AAATCTGGCA ACATCTTGCT GCATAAGTCA 
ACCATTAACA GGGAGGCTAC CCGGTTACGG AGCCGATGGC TGACATATTT CATGGCCGTC 
AAGTCCAATG GTAGCGTCTT TGTTCGAGAT TCCTCCCAGG TGCACCCACT AGCTGTGTTG 
CTCCTAACAG ATGGGGACGT GCACATCCGA GATGATGGGC GTCGGGCCAC CATCTCATTG 
AGTGACAGCG ACCTGCTTCG GCTGGAAGGT GATTCACGAA CTGTGCGGTT GCTAAGGGAG 
TTTCGTCGAG CCCTAGGACG GATGGTGGAG CGGAGCCTCC GCAGCGAGTT AGCTGCACTT 
CCTCTTAGTG TGCAGCAAGA ACACGGGCAG CTGCTTGCAC TGCTGGCAGA GTTGCTTCGA 
GGACCTTGTG GCAGCTTTGA TATGCGCAAG ACAGCTGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_006512304.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512367.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X10, mRNA.

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CloneID	OMu12947
Clone ID Related Accession (Same CDS sequence)	XM_006512303.2 , XM_006512303.1 , XM_030244640.1 , XM_030244641.1 , XM_006512304.1 , XM_006512305.3 , NM_001252682.1 , XM_006512305.4
Accession Version	XM_006512304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCT TGGACTCATT CAGAAAAGAT CGGACCCAGC ACAGGCAGCG TCAATGCAAG 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCACT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCGCAGCCTA AAAACCTTCT CAACAGCGTG 
ATTGGAAGAG CCCTTGGCAT CTCACATGCA AAAGACAAGT TAGTCTATGT GCACACGAAT 
GGACCGAAGA AAAAGAAAGT CACCCTGCAC ATAAAGTGGC CCAAGAGCGT GGAGGTGGAA 
GGCTATGGCA GCAAGAAGAT TGATGCTGAG CGTCAGGCTG CAGCAGCTGC CTGCCAACTC 
TTCAAGGGCT GGGGTCTGCT GGGACCACGG AATGAGCTGT TTGATGCAGC TAAATACCGA 
GTGCTAGCTG ATCGTTTTGG GTCTCCAGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCTCCAACTT CCTGGCGGCA GCTGAATCCT GAGAACATTC GGCCAGGGGG TCCTGCAGGA 
CTATCCCGAT CCTTAGGCCG AGAGGAAGAG GAAGATGAGG AGGAAGAGCT AGAAGAGGGG 
ACCATTGATG TGACAGAGTT TCTGTCTATG ACCCAGCAAG ACTCCCACAA CCCACTCAGG 
GACTCAAGGG GGGGCTCCTT TGAAATGACA GATGATGACA GTGCTATCAG AGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTCCTGGCC AAAGTGATTC AGATTGCAAC CTCCTCCTCC 
ACAGCTAAGA ATCTCATGCA GTTCCATACT GTGGGTACCA AGACCAAGCT GGCTACACTC 
ACTCTGCTCT GGCCCTGTCC CATGACCTTT GTTGCCAAAG GGCGACGCAA AGCTGAGGCT 
GAGAATAAGG CAGCAGCCTT GGCTTGCAAG AAACTGAAGA GCCTTGGCCT TGTGGACAGG 
AACAATGAGC CGCTTACCCA TGCCATGTAC AACCTGGCCT CCCTGCGTGA GTTGGGTGAG 
ACCCAGCGCC GGCCATGTAC CATCCAGGTG CCTGAGCCCA TCCTTCGCAA GATAGAGGCC 
TTCCTGAGTC ATTACCCGGT GGACAGCTCA TGGATTTCCC CAGAACTCCG ACTGCAGAGT 
GATGACATCT TGCCCTTAGG CAAGGACTCA GGGCCCTTGA GTGACCCTAT CACAGGCAAG 
CCATACATGC CCCTGTCAGA AGCAGAGGAG GTGCGCTTAA GCCAGAGCCT GCTAGAGCTG 
TGGCGGAGGA GAGGGCCGAT CTGGCAGGAG GCCCCGCAGC TACCTGTAGA CCCTCATCGG 
GACACTATCC TCAGTGCCAT TGAACAACAC CCAGTGGTAG TCATCTCTGG GGACACAGGC 
TGTGGGAAGA CCACACGTAT CCCTCAGCTG CTATTGGAGC GCTATGTGAC TGAGGGTCGA 
GGTGCCCGCT GCAATGTGAT CATCACACAA CCTCGCCGGA TCTCAGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCATGAACT GGGCCCCTCC TTGCGCCGGA ATGTGGGCTT CCAGGTACGC 
TTGGAAAGTA AGCCCCCAGC CCGAGGTGGG GCGCTGCTCT TCTGCACTGT GGGTATCCTG 
CTTCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGTGTGA GCCATGTCAT TGTGGATGAG 
GTCCATGAGC GGGATGTGAA CACAGACTTC CTGCTGATTC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCAG CTCTTCGGCT GGTGCTCATG AGTGCTACAG GAGATAATGA GCGCTTTTCC 
CGATACTTTG GGGGCTGCCC TGTTATCAAG GTACCTGGCT TCATGTATCC CGTCAAGGAA 
CACTACCTGG AGGACATCTT GGCCAAGCTG GGTAAACATC AGTACCCACA CCGGCATCGG 
CACCATGAGT CGGAAGATGA ATGTGCACTT GATTTGGACC TTGTGACTGA CCTGGTTCTG 
CATATCGATG CCCGTGGGGA ACCAGGTGGG ATCCTGTGCT TTCTACCTGG CTGGCAAGAA 
ATCAAAGGAG TGCAACAACG GCTCCAGGAG GCCCTGGGCA TGCATGAAAG CAAGTACCTC 
ATCTTACCAG TGCACTCCAA TATCCCCATG ATGGACCAGA AGGCCATATT CCAACAGCCT 
CCACTTGGGG TACGCAAGAT TGTATTGGCC ACCAACATTG CTGAAACCTC CATCACAGTT 
AACGACATTG TACATGTCGT GGACAGCGGT CTGCACAAGG AGGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACTGTGTGG GTGTCGAGAG CAAATGTCAT TCAGCGCCGG 
GGCAGGGCAG GCCGCTGCCA GTCAGGTTTT GCCTACCACT TGTTCCCGAG GAGCCGGCTG 
GAGAAAATGG TTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCCA AAATCCATAT GCCTGAGAAG ACGGCAGTGG AGTTCCTCTC TAAGGCTGTG 
GACAGTCCAA ATATCAAGGC AGTGGATGAG GCCGTGATCC TGCTCCAGGA GATTGGGGTG 
CTGGACCAGC GGGAGTATCT GACCACCTTG GGACAGCGCC TGGCCCACAT CTCTACTGAC 
CCCCGACTGG CCAAGGCCAT AGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTGCTG 
GTGGTTGTTT CCTGCCTTAC CCGGGACCCC TTCAGCAGCA GTTTGCAGAA CCGGGCAGAA 
GTAGACAAGG TGAAGGCATT GCTGAGCCAT GACAGTGGCA GTGACCATTT GGCCTTCGTG 
CGGGCTGTGG CTGGCTGGGA GGAGGTACTG CGCTGGCAGG ACCGTACCTC CAGGGAAAAC 
TACCTGGAAG AAAACCTTCT GTATGCCCCC AGCTTGCGCT TCATCCACGG GCTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTAGTGGGGA AGCCCTCTGA CTGCACACTG 
CCCTCTGCTC AGTGCAATGA GTACAGCGAG GAAGAGGAGC TGGTGAAGGG TGTGCTGATG 
GCTGGCCTCT ACCCCAACCT CATCCAGGTG AGACAAGGTA AGGTTACTCG GCAAGGCAAG 
TTCAAACCCA ACAGTGTCAC TTACAGGACC AAATCTGGCA ACATCTTGCT GCATAAGTCA 
ACCATTAACA GGGAGGCTAC CCGGTTACGG AGCCGATGGC TGACATATTT CATGGCCGTC 
AAGTCCAATG GTAGCGTCTT TGTTCGAGAT TCCTCCCAGG TGCACCCACT AGCTGTGTTG 
CTCCTAACAG ATGGGGACGT GCACATCCGA GATGATGGGC GTCGGGCCAC CATCTCATTG 
AGTGACAGCG ACCTGCTTCG GCTGGAAGGT GATTCACGAA CTGTGCGGTT GCTAAGGGAG 
TTTCGTCGAG CCCTAGGACG GATGGTGGAG CGGAGCCTCC GCAGCGAGTT AGCTGCACTT 
CCTCTTAGTG TGCAGCAAGA ACACGGGCAG CTGCTTGCAC TGCTGGCAGA GTTGCTTCGA 
GGACCTTGTG GCAGCTTTGA TATGCGCAAG ACAGCTGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512367.1
CDS	814..4398
Translation	MFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_006512304.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512304.1

ORF Insert Sequence:

ATGTTCAGCT TGGACTCATT CAGAAAAGAT CGGACCCAGC ACAGGCAGCG TCAATGCAAG 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCACT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCGCAGCCTA AAAACCTTCT CAACAGCGTG 
ATTGGAAGAG CCCTTGGCAT CTCACATGCA AAAGACAAGT TAGTCTATGT GCACACGAAT 
GGACCGAAGA AAAAGAAAGT CACCCTGCAC ATAAAGTGGC CCAAGAGCGT GGAGGTGGAA 
GGCTATGGCA GCAAGAAGAT TGATGCTGAG CGTCAGGCTG CAGCAGCTGC CTGCCAACTC 
TTCAAGGGCT GGGGTCTGCT GGGACCACGG AATGAGCTGT TTGATGCAGC TAAATACCGA 
GTGCTAGCTG ATCGTTTTGG GTCTCCAGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCTCCAACTT CCTGGCGGCA GCTGAATCCT GAGAACATTC GGCCAGGGGG TCCTGCAGGA 
CTATCCCGAT CCTTAGGCCG AGAGGAAGAG GAAGATGAGG AGGAAGAGCT AGAAGAGGGG 
ACCATTGATG TGACAGAGTT TCTGTCTATG ACCCAGCAAG ACTCCCACAA CCCACTCAGG 
GACTCAAGGG GGGGCTCCTT TGAAATGACA GATGATGACA GTGCTATCAG AGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTCCTGGCC AAAGTGATTC AGATTGCAAC CTCCTCCTCC 
ACAGCTAAGA ATCTCATGCA GTTCCATACT GTGGGTACCA AGACCAAGCT GGCTACACTC 
ACTCTGCTCT GGCCCTGTCC CATGACCTTT GTTGCCAAAG GGCGACGCAA AGCTGAGGCT 
GAGAATAAGG CAGCAGCCTT GGCTTGCAAG AAACTGAAGA GCCTTGGCCT TGTGGACAGG 
AACAATGAGC CGCTTACCCA TGCCATGTAC AACCTGGCCT CCCTGCGTGA GTTGGGTGAG 
ACCCAGCGCC GGCCATGTAC CATCCAGGTG CCTGAGCCCA TCCTTCGCAA GATAGAGGCC 
TTCCTGAGTC ATTACCCGGT GGACAGCTCA TGGATTTCCC CAGAACTCCG ACTGCAGAGT 
GATGACATCT TGCCCTTAGG CAAGGACTCA GGGCCCTTGA GTGACCCTAT CACAGGCAAG 
CCATACATGC CCCTGTCAGA AGCAGAGGAG GTGCGCTTAA GCCAGAGCCT GCTAGAGCTG 
TGGCGGAGGA GAGGGCCGAT CTGGCAGGAG GCCCCGCAGC TACCTGTAGA CCCTCATCGG 
GACACTATCC TCAGTGCCAT TGAACAACAC CCAGTGGTAG TCATCTCTGG GGACACAGGC 
TGTGGGAAGA CCACACGTAT CCCTCAGCTG CTATTGGAGC GCTATGTGAC TGAGGGTCGA 
GGTGCCCGCT GCAATGTGAT CATCACACAA CCTCGCCGGA TCTCAGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCATGAACT GGGCCCCTCC TTGCGCCGGA ATGTGGGCTT CCAGGTACGC 
TTGGAAAGTA AGCCCCCAGC CCGAGGTGGG GCGCTGCTCT TCTGCACTGT GGGTATCCTG 
CTTCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGTGTGA GCCATGTCAT TGTGGATGAG 
GTCCATGAGC GGGATGTGAA CACAGACTTC CTGCTGATTC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCAG CTCTTCGGCT GGTGCTCATG AGTGCTACAG GAGATAATGA GCGCTTTTCC 
CGATACTTTG GGGGCTGCCC TGTTATCAAG GTACCTGGCT TCATGTATCC CGTCAAGGAA 
CACTACCTGG AGGACATCTT GGCCAAGCTG GGTAAACATC AGTACCCACA CCGGCATCGG 
CACCATGAGT CGGAAGATGA ATGTGCACTT GATTTGGACC TTGTGACTGA CCTGGTTCTG 
CATATCGATG CCCGTGGGGA ACCAGGTGGG ATCCTGTGCT TTCTACCTGG CTGGCAAGAA 
ATCAAAGGAG TGCAACAACG GCTCCAGGAG GCCCTGGGCA TGCATGAAAG CAAGTACCTC 
ATCTTACCAG TGCACTCCAA TATCCCCATG ATGGACCAGA AGGCCATATT CCAACAGCCT 
CCACTTGGGG TACGCAAGAT TGTATTGGCC ACCAACATTG CTGAAACCTC CATCACAGTT 
AACGACATTG TACATGTCGT GGACAGCGGT CTGCACAAGG AGGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACTGTGTGG GTGTCGAGAG CAAATGTCAT TCAGCGCCGG 
GGCAGGGCAG GCCGCTGCCA GTCAGGTTTT GCCTACCACT TGTTCCCGAG GAGCCGGCTG 
GAGAAAATGG TTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCCA AAATCCATAT GCCTGAGAAG ACGGCAGTGG AGTTCCTCTC TAAGGCTGTG 
GACAGTCCAA ATATCAAGGC AGTGGATGAG GCCGTGATCC TGCTCCAGGA GATTGGGGTG 
CTGGACCAGC GGGAGTATCT GACCACCTTG GGACAGCGCC TGGCCCACAT CTCTACTGAC 
CCCCGACTGG CCAAGGCCAT AGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTGCTG 
GTGGTTGTTT CCTGCCTTAC CCGGGACCCC TTCAGCAGCA GTTTGCAGAA CCGGGCAGAA 
GTAGACAAGG TGAAGGCATT GCTGAGCCAT GACAGTGGCA GTGACCATTT GGCCTTCGTG 
CGGGCTGTGG CTGGCTGGGA GGAGGTACTG CGCTGGCAGG ACCGTACCTC CAGGGAAAAC 
TACCTGGAAG AAAACCTTCT GTATGCCCCC AGCTTGCGCT TCATCCACGG GCTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTAGTGGGGA AGCCCTCTGA CTGCACACTG 
CCCTCTGCTC AGTGCAATGA GTACAGCGAG GAAGAGGAGC TGGTGAAGGG TGTGCTGATG 
GCTGGCCTCT ACCCCAACCT CATCCAGGTG AGACAAGGTA AGGTTACTCG GCAAGGCAAG 
TTCAAACCCA ACAGTGTCAC TTACAGGACC AAATCTGGCA ACATCTTGCT GCATAAGTCA 
ACCATTAACA GGGAGGCTAC CCGGTTACGG AGCCGATGGC TGACATATTT CATGGCCGTC 
AAGTCCAATG GTAGCGTCTT TGTTCGAGAT TCCTCCCAGG TGCACCCACT AGCTGTGTTG 
CTCCTAACAG ATGGGGACGT GCACATCCGA GATGATGGGC GTCGGGCCAC CATCTCATTG 
AGTGACAGCG ACCTGCTTCG GCTGGAAGGT GATTCACGAA CTGTGCGGTT GCTAAGGGAG 
TTTCGTCGAG CCCTAGGACG GATGGTGGAG CGGAGCCTCC GCAGCGAGTT AGCTGCACTT 
CCTCTTAGTG TGCAGCAAGA ACACGGGCAG CTGCTTGCAC TGCTGGCAGA GTTGCTTCGA 
GGACCTTGTG GCAGCTTTGA TATGCGCAAG ACAGCTGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_006512305.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512368.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X10, mRNA.

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CloneID	OMu12947
Clone ID Related Accession (Same CDS sequence)	XM_006512303.2 , XM_006512303.1 , XM_030244640.1 , XM_030244641.1 , XM_006512304.1 , XM_006512305.3 , NM_001252682.1 , XM_006512305.4
Accession Version	XM_006512305.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	putative ATP-dependent RNA helicase DHX30 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512305.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCT TGGACTCATT CAGAAAAGAT CGGACCCAGC ACAGGCAGCG TCAATGCAAG 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCACT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCGCAGCCTA AAAACCTTCT CAACAGCGTG 
ATTGGAAGAG CCCTTGGCAT CTCACATGCA AAAGACAAGT TAGTCTATGT GCACACGAAT 
GGACCGAAGA AAAAGAAAGT CACCCTGCAC ATAAAGTGGC CCAAGAGCGT GGAGGTGGAA 
GGCTATGGCA GCAAGAAGAT TGATGCTGAG CGTCAGGCTG CAGCAGCTGC CTGCCAACTC 
TTCAAGGGCT GGGGTCTGCT GGGACCACGG AATGAGCTGT TTGATGCAGC TAAATACCGA 
GTGCTAGCTG ATCGTTTTGG GTCTCCAGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCTCCAACTT CCTGGCGGCA GCTGAATCCT GAGAACATTC GGCCAGGGGG TCCTGCAGGA 
CTATCCCGAT CCTTAGGCCG AGAGGAAGAG GAAGATGAGG AGGAAGAGCT AGAAGAGGGG 
ACCATTGATG TGACAGAGTT TCTGTCTATG ACCCAGCAAG ACTCCCACAA CCCACTCAGG 
GACTCAAGGG GGGGCTCCTT TGAAATGACA GATGATGACA GTGCTATCAG AGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTCCTGGCC AAAGTGATTC AGATTGCAAC CTCCTCCTCC 
ACAGCTAAGA ATCTCATGCA GTTCCATACT GTGGGTACCA AGACCAAGCT GGCTACACTC 
ACTCTGCTCT GGCCCTGTCC CATGACCTTT GTTGCCAAAG GGCGACGCAA AGCTGAGGCT 
GAGAATAAGG CAGCAGCCTT GGCTTGCAAG AAACTGAAGA GCCTTGGCCT TGTGGACAGG 
AACAATGAGC CGCTTACCCA TGCCATGTAC AACCTGGCCT CCCTGCGTGA GTTGGGTGAG 
ACCCAGCGCC GGCCATGTAC CATCCAGGTG CCTGAGCCCA TCCTTCGCAA GATAGAGGCC 
TTCCTGAGTC ATTACCCGGT GGACAGCTCA TGGATTTCCC CAGAACTCCG ACTGCAGAGT 
GATGACATCT TGCCCTTAGG CAAGGACTCA GGGCCCTTGA GTGACCCTAT CACAGGCAAG 
CCATACATGC CCCTGTCAGA AGCAGAGGAG GTGCGCTTAA GCCAGAGCCT GCTAGAGCTG 
TGGCGGAGGA GAGGGCCGAT CTGGCAGGAG GCCCCGCAGC TACCTGTAGA CCCTCATCGG 
GACACTATCC TCAGTGCCAT TGAACAACAC CCAGTGGTAG TCATCTCTGG GGACACAGGC 
TGTGGGAAGA CCACACGTAT CCCTCAGCTG CTATTGGAGC GCTATGTGAC TGAGGGTCGA 
GGTGCCCGCT GCAATGTGAT CATCACACAA CCTCGCCGGA TCTCAGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCATGAACT GGGCCCCTCC TTGCGCCGGA ATGTGGGCTT CCAGGTACGC 
TTGGAAAGTA AGCCCCCAGC CCGAGGTGGG GCGCTGCTCT TCTGCACTGT GGGTATCCTG 
CTTCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGTGTGA GCCATGTCAT TGTGGATGAG 
GTCCATGAGC GGGATGTGAA CACAGACTTC CTGCTGATTC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCAG CTCTTCGGCT GGTGCTCATG AGTGCTACAG GAGATAATGA GCGCTTTTCC 
CGATACTTTG GGGGCTGCCC TGTTATCAAG GTACCTGGCT TCATGTATCC CGTCAAGGAA 
CACTACCTGG AGGACATCTT GGCCAAGCTG GGTAAACATC AGTACCCACA CCGGCATCGG 
CACCATGAGT CGGAAGATGA ATGTGCACTT GATTTGGACC TTGTGACTGA CCTGGTTCTG 
CATATCGATG CCCGTGGGGA ACCAGGTGGG ATCCTGTGCT TTCTACCTGG CTGGCAAGAA 
ATCAAAGGAG TGCAACAACG GCTCCAGGAG GCCCTGGGCA TGCATGAAAG CAAGTACCTC 
ATCTTACCAG TGCACTCCAA TATCCCCATG ATGGACCAGA AGGCCATATT CCAACAGCCT 
CCACTTGGGG TACGCAAGAT TGTATTGGCC ACCAACATTG CTGAAACCTC CATCACAGTT 
AACGACATTG TACATGTCGT GGACAGCGGT CTGCACAAGG AGGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACTGTGTGG GTGTCGAGAG CAAATGTCAT TCAGCGCCGG 
GGCAGGGCAG GCCGCTGCCA GTCAGGTTTT GCCTACCACT TGTTCCCGAG GAGCCGGCTG 
GAGAAAATGG TTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCCA AAATCCATAT GCCTGAGAAG ACGGCAGTGG AGTTCCTCTC TAAGGCTGTG 
GACAGTCCAA ATATCAAGGC AGTGGATGAG GCCGTGATCC TGCTCCAGGA GATTGGGGTG 
CTGGACCAGC GGGAGTATCT GACCACCTTG GGACAGCGCC TGGCCCACAT CTCTACTGAC 
CCCCGACTGG CCAAGGCCAT AGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTGCTG 
GTGGTTGTTT CCTGCCTTAC CCGGGACCCC TTCAGCAGCA GTTTGCAGAA CCGGGCAGAA 
GTAGACAAGG TGAAGGCATT GCTGAGCCAT GACAGTGGCA GTGACCATTT GGCCTTCGTG 
CGGGCTGTGG CTGGCTGGGA GGAGGTACTG CGCTGGCAGG ACCGTACCTC CAGGGAAAAC 
TACCTGGAAG AAAACCTTCT GTATGCCCCC AGCTTGCGCT TCATCCACGG GCTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTAGTGGGGA AGCCCTCTGA CTGCACACTG 
CCCTCTGCTC AGTGCAATGA GTACAGCGAG GAAGAGGAGC TGGTGAAGGG TGTGCTGATG 
GCTGGCCTCT ACCCCAACCT CATCCAGGTG AGACAAGGTA AGGTTACTCG GCAAGGCAAG 
TTCAAACCCA ACAGTGTCAC TTACAGGACC AAATCTGGCA ACATCTTGCT GCATAAGTCA 
ACCATTAACA GGGAGGCTAC CCGGTTACGG AGCCGATGGC TGACATATTT CATGGCCGTC 
AAGTCCAATG GTAGCGTCTT TGTTCGAGAT TCCTCCCAGG TGCACCCACT AGCTGTGTTG 
CTCCTAACAG ATGGGGACGT GCACATCCGA GATGATGGGC GTCGGGCCAC CATCTCATTG 
AGTGACAGCG ACCTGCTTCG GCTGGAAGGT GATTCACGAA CTGTGCGGTT GCTAAGGGAG 
TTTCGTCGAG CCCTAGGACG GATGGTGGAG CGGAGCCTCC GCAGCGAGTT AGCTGCACTT 
CCTCTTAGTG TGCAGCAAGA ACACGGGCAG CTGCTTGCAC TGCTGGCAGA GTTGCTTCGA 
GGACCTTGTG GCAGCTTTGA TATGCGCAAG ACAGCTGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512368.1
CDS	557..4141
Translation	MFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_006512305.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512305.3

ORF Insert Sequence:

ATGTTCAGCT TGGACTCATT CAGAAAAGAT CGGACCCAGC ACAGGCAGCG TCAATGCAAG 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCACT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCGCAGCCTA AAAACCTTCT CAACAGCGTG 
ATTGGAAGAG CCCTTGGCAT CTCACATGCA AAAGACAAGT TAGTCTATGT GCACACGAAT 
GGACCGAAGA AAAAGAAAGT CACCCTGCAC ATAAAGTGGC CCAAGAGCGT GGAGGTGGAA 
GGCTATGGCA GCAAGAAGAT TGATGCTGAG CGTCAGGCTG CAGCAGCTGC CTGCCAACTC 
TTCAAGGGCT GGGGTCTGCT GGGACCACGG AATGAGCTGT TTGATGCAGC TAAATACCGA 
GTGCTAGCTG ATCGTTTTGG GTCTCCAGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCTCCAACTT CCTGGCGGCA GCTGAATCCT GAGAACATTC GGCCAGGGGG TCCTGCAGGA 
CTATCCCGAT CCTTAGGCCG AGAGGAAGAG GAAGATGAGG AGGAAGAGCT AGAAGAGGGG 
ACCATTGATG TGACAGAGTT TCTGTCTATG ACCCAGCAAG ACTCCCACAA CCCACTCAGG 
GACTCAAGGG GGGGCTCCTT TGAAATGACA GATGATGACA GTGCTATCAG AGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTCCTGGCC AAAGTGATTC AGATTGCAAC CTCCTCCTCC 
ACAGCTAAGA ATCTCATGCA GTTCCATACT GTGGGTACCA AGACCAAGCT GGCTACACTC 
ACTCTGCTCT GGCCCTGTCC CATGACCTTT GTTGCCAAAG GGCGACGCAA AGCTGAGGCT 
GAGAATAAGG CAGCAGCCTT GGCTTGCAAG AAACTGAAGA GCCTTGGCCT TGTGGACAGG 
AACAATGAGC CGCTTACCCA TGCCATGTAC AACCTGGCCT CCCTGCGTGA GTTGGGTGAG 
ACCCAGCGCC GGCCATGTAC CATCCAGGTG CCTGAGCCCA TCCTTCGCAA GATAGAGGCC 
TTCCTGAGTC ATTACCCGGT GGACAGCTCA TGGATTTCCC CAGAACTCCG ACTGCAGAGT 
GATGACATCT TGCCCTTAGG CAAGGACTCA GGGCCCTTGA GTGACCCTAT CACAGGCAAG 
CCATACATGC CCCTGTCAGA AGCAGAGGAG GTGCGCTTAA GCCAGAGCCT GCTAGAGCTG 
TGGCGGAGGA GAGGGCCGAT CTGGCAGGAG GCCCCGCAGC TACCTGTAGA CCCTCATCGG 
GACACTATCC TCAGTGCCAT TGAACAACAC CCAGTGGTAG TCATCTCTGG GGACACAGGC 
TGTGGGAAGA CCACACGTAT CCCTCAGCTG CTATTGGAGC GCTATGTGAC TGAGGGTCGA 
GGTGCCCGCT GCAATGTGAT CATCACACAA CCTCGCCGGA TCTCAGCTGT GTCTGTGGCA 
CAGCGGGTCA GCCATGAACT GGGCCCCTCC TTGCGCCGGA ATGTGGGCTT CCAGGTACGC 
TTGGAAAGTA AGCCCCCAGC CCGAGGTGGG GCGCTGCTCT TCTGCACTGT GGGTATCCTG 
CTTCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGTGTGA GCCATGTCAT TGTGGATGAG 
GTCCATGAGC GGGATGTGAA CACAGACTTC CTGCTGATTC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCAG CTCTTCGGCT GGTGCTCATG AGTGCTACAG GAGATAATGA GCGCTTTTCC 
CGATACTTTG GGGGCTGCCC TGTTATCAAG GTACCTGGCT TCATGTATCC CGTCAAGGAA 
CACTACCTGG AGGACATCTT GGCCAAGCTG GGTAAACATC AGTACCCACA CCGGCATCGG 
CACCATGAGT CGGAAGATGA ATGTGCACTT GATTTGGACC TTGTGACTGA CCTGGTTCTG 
CATATCGATG CCCGTGGGGA ACCAGGTGGG ATCCTGTGCT TTCTACCTGG CTGGCAAGAA 
ATCAAAGGAG TGCAACAACG GCTCCAGGAG GCCCTGGGCA TGCATGAAAG CAAGTACCTC 
ATCTTACCAG TGCACTCCAA TATCCCCATG ATGGACCAGA AGGCCATATT CCAACAGCCT 
CCACTTGGGG TACGCAAGAT TGTATTGGCC ACCAACATTG CTGAAACCTC CATCACAGTT 
AACGACATTG TACATGTCGT GGACAGCGGT CTGCACAAGG AGGAACGCTA TGACCTGAAG 
ACCAAGGTGT CCTGCCTGGA GACTGTGTGG GTGTCGAGAG CAAATGTCAT TCAGCGCCGG 
GGCAGGGCAG GCCGCTGCCA GTCAGGTTTT GCCTACCACT TGTTCCCGAG GAGCCGGCTG 
GAGAAAATGG TTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCTCTTGA GAACCTGGTG 
CTGCAAGCCA AAATCCATAT GCCTGAGAAG ACGGCAGTGG AGTTCCTCTC TAAGGCTGTG 
GACAGTCCAA ATATCAAGGC AGTGGATGAG GCCGTGATCC TGCTCCAGGA GATTGGGGTG 
CTGGACCAGC GGGAGTATCT GACCACCTTG GGACAGCGCC TGGCCCACAT CTCTACTGAC 
CCCCGACTGG CCAAGGCCAT AGTGTTGGCT GCCATCTTCC GTTGCCTGCA CCCACTGCTG 
GTGGTTGTTT CCTGCCTTAC CCGGGACCCC TTCAGCAGCA GTTTGCAGAA CCGGGCAGAA 
GTAGACAAGG TGAAGGCATT GCTGAGCCAT GACAGTGGCA GTGACCATTT GGCCTTCGTG 
CGGGCTGTGG CTGGCTGGGA GGAGGTACTG CGCTGGCAGG ACCGTACCTC CAGGGAAAAC 
TACCTGGAAG AAAACCTTCT GTATGCCCCC AGCTTGCGCT TCATCCACGG GCTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTAGTGGGGA AGCCCTCTGA CTGCACACTG 
CCCTCTGCTC AGTGCAATGA GTACAGCGAG GAAGAGGAGC TGGTGAAGGG TGTGCTGATG 
GCTGGCCTCT ACCCCAACCT CATCCAGGTG AGACAAGGTA AGGTTACTCG GCAAGGCAAG 
TTCAAACCCA ACAGTGTCAC TTACAGGACC AAATCTGGCA ACATCTTGCT GCATAAGTCA 
ACCATTAACA GGGAGGCTAC CCGGTTACGG AGCCGATGGC TGACATATTT CATGGCCGTC 
AAGTCCAATG GTAGCGTCTT TGTTCGAGAT TCCTCCCAGG TGCACCCACT AGCTGTGTTG 
CTCCTAACAG ATGGGGACGT GCACATCCGA GATGATGGGC GTCGGGCCAC CATCTCATTG 
AGTGACAGCG ACCTGCTTCG GCTGGAAGGT GATTCACGAA CTGTGCGGTT GCTAAGGGAG 
TTTCGTCGAG CCCTAGGACG GATGGTGGAG CGGAGCCTCC GCAGCGAGTT AGCTGCACTT 
CCTCTTAGTG TGCAGCAAGA ACACGGGCAG CTGCTTGCAC TGCTGGCAGA GTTGCTTCGA 
GGACCTTGTG GCAGCTTTGA TATGCGCAAG ACAGCTGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_017313613.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169102.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X3, mRNA.

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CloneID	OMu13452
Clone ID Related Accession (Same CDS sequence)	XM_017313613.1 , XM_006512301.3 , XM_006512301.4 , XM_011243029.2 , XM_006512300.1 , XM_011243029.3 , XM_006512300.2 , XM_017313614.1 , XM_006512299.2 , XM_006512298.4 , NM_133347.2 , XM_006512299.1 , XM_006512298.3
Accession Version	XM_017313613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	putative ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169102.1
CDS	716..4369
Translation	MVTPVCNSSTWQPKDSSFLSWPEMFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_017313613.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313613.1

ORF Insert Sequence:

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_006512301.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512364.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X7, mRNA.

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CloneID	OMu13452
Clone ID Related Accession (Same CDS sequence)	XM_017313613.1 , XM_006512301.3 , XM_006512301.4 , XM_011243029.2 , XM_006512300.1 , XM_011243029.3 , XM_006512300.2 , XM_017313614.1 , XM_006512299.2 , XM_006512298.4 , NM_133347.2 , XM_006512299.1 , XM_006512298.3
Accession Version	XM_006512301.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	putative ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512301.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512364.1
CDS	423..4076
Translation	MVTPVCNSSTWQPKDSSFLSWPEMFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_006512301.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512301.3

ORF Insert Sequence:

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_011243029.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241331.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X4, mRNA.

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CloneID	OMu13452
Clone ID Related Accession (Same CDS sequence)	XM_017313613.1 , XM_006512301.3 , XM_006512301.4 , XM_011243029.2 , XM_006512300.1 , XM_011243029.3 , XM_006512300.2 , XM_017313614.1 , XM_006512299.2 , XM_006512298.4 , NM_133347.2 , XM_006512299.1 , XM_006512298.3
Accession Version	XM_011243029.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	putative ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243029.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241331.1
CDS	502..4155
Translation	MVTPVCNSSTWQPKDSSFLSWPEMFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_011243029.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243029.2

ORF Insert Sequence:

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_006512300.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512363.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X6, mRNA.

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CloneID	OMu13452
Clone ID Related Accession (Same CDS sequence)	XM_017313613.1 , XM_006512301.3 , XM_006512301.4 , XM_011243029.2 , XM_006512300.1 , XM_011243029.3 , XM_006512300.2 , XM_017313614.1 , XM_006512299.2 , XM_006512298.4 , NM_133347.2 , XM_006512299.1 , XM_006512298.3
Accession Version	XM_006512300.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	putative ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512363.1
CDS	680..4333
Translation	MVTPVCNSSTWQPKDSSFLSWPEMFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_006512300.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512300.1

ORF Insert Sequence:

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_017313614.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169103.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X5, mRNA.

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CloneID	OMu13452
Clone ID Related Accession (Same CDS sequence)	XM_017313613.1 , XM_006512301.3 , XM_006512301.4 , XM_011243029.2 , XM_006512300.1 , XM_011243029.3 , XM_006512300.2 , XM_017313614.1 , XM_006512299.2 , XM_006512298.4 , NM_133347.2 , XM_006512299.1 , XM_006512298.3
Accession Version	XM_017313614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169103.1
CDS	835..4488
Translation	MVTPVCNSSTWQPKDSSFLSWPEMFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_017313614.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313614.1

ORF Insert Sequence:

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_006512299.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512362.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X1, mRNA.

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CloneID	OMu13452
Clone ID Related Accession (Same CDS sequence)	XM_017313613.1 , XM_006512301.3 , XM_006512301.4 , XM_011243029.2 , XM_006512300.1 , XM_011243029.3 , XM_006512300.2 , XM_017313614.1 , XM_006512299.2 , XM_006512298.4 , NM_133347.2 , XM_006512299.1 , XM_006512298.3
Accession Version	XM_006512299.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	putative ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512362.1
CDS	841..4494
Translation	MVTPVCNSSTWQPKDSSFLSWPEMFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_006512299.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512299.1

ORF Insert Sequence:

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_006512298.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512361.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X2, mRNA.

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CloneID	OMu13452
Clone ID Related Accession (Same CDS sequence)	XM_017313613.1 , XM_006512301.3 , XM_006512301.4 , XM_011243029.2 , XM_006512300.1 , XM_011243029.3 , XM_006512300.2 , XM_017313614.1 , XM_006512299.2 , XM_006512298.4 , NM_133347.2 , XM_006512299.1 , XM_006512298.3
Accession Version	XM_006512298.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	putative ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512298.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512361.1
CDS	387..4040
Translation	MVTPVCNSSTWQPKDSSFLSWPEMFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_006512298.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512298.3

ORF Insert Sequence:

ATGGTGACTC CTGTCTGTAA TTCCAGCACT TGGCAGCCCA AGGATTCCAG CTTTCTCTCC 
TGGCCAGAAA TGTTCAGCTT GGACTCATTC AGAAAAGATC GGACCCAGCA CAGGCAGCGT 
CAATGCAAGC TTCCCCCACC CCGTCTTCCA CCCATGTGTG TCAACCCTGC CCCTGGAGGG 
ACCATCACTC GAGCTTCTAG GGACCTATTA AAAGAGTTTC CGCAGCCTAA AAACCTTCTC 
AACAGCGTGA TTGGAAGAGC CCTTGGCATC TCACATGCAA AAGACAAGTT AGTCTATGTG 
CACACGAATG GACCGAAGAA AAAGAAAGTC ACCCTGCACA TAAAGTGGCC CAAGAGCGTG 
GAGGTGGAAG GCTATGGCAG CAAGAAGATT GATGCTGAGC GTCAGGCTGC AGCAGCTGCC 
TGCCAACTCT TCAAGGGCTG GGGTCTGCTG GGACCACGGA ATGAGCTGTT TGATGCAGCT 
AAATACCGAG TGCTAGCTGA TCGTTTTGGG TCTCCAGCTG ACAGCTGGTG GCGCCCAGAA 
CCCACCATGC CTCCAACTTC CTGGCGGCAG CTGAATCCTG AGAACATTCG GCCAGGGGGT 
CCTGCAGGAC TATCCCGATC CTTAGGCCGA GAGGAAGAGG AAGATGAGGA GGAAGAGCTA 
GAAGAGGGGA CCATTGATGT GACAGAGTTT CTGTCTATGA CCCAGCAAGA CTCCCACAAC 
CCACTCAGGG ACTCAAGGGG GGGCTCCTTT GAAATGACAG ATGATGACAG TGCTATCAGA 
GCTCTGACCC AGTTTCCACT TCCCAAGAAC CTCCTGGCCA AAGTGATTCA GATTGCAACC 
TCCTCCTCCA CAGCTAAGAA TCTCATGCAG TTCCATACTG TGGGTACCAA GACCAAGCTG 
GCTACACTCA CTCTGCTCTG GCCCTGTCCC ATGACCTTTG TTGCCAAAGG GCGACGCAAA 
GCTGAGGCTG AGAATAAGGC AGCAGCCTTG GCTTGCAAGA AACTGAAGAG CCTTGGCCTT 
GTGGACAGGA ACAATGAGCC GCTTACCCAT GCCATGTACA ACCTGGCCTC CCTGCGTGAG 
TTGGGTGAGA CCCAGCGCCG GCCATGTACC ATCCAGGTGC CTGAGCCCAT CCTTCGCAAG 
ATAGAGGCCT TCCTGAGTCA TTACCCGGTG GACAGCTCAT GGATTTCCCC AGAACTCCGA 
CTGCAGAGTG ATGACATCTT GCCCTTAGGC AAGGACTCAG GGCCCTTGAG TGACCCTATC 
ACAGGCAAGC CATACATGCC CCTGTCAGAA GCAGAGGAGG TGCGCTTAAG CCAGAGCCTG 
CTAGAGCTGT GGCGGAGGAG AGGGCCGATC TGGCAGGAGG CCCCGCAGCT ACCTGTAGAC 
CCTCATCGGG ACACTATCCT CAGTGCCATT GAACAACACC CAGTGGTAGT CATCTCTGGG 
GACACAGGCT GTGGGAAGAC CACACGTATC CCTCAGCTGC TATTGGAGCG CTATGTGACT 
GAGGGTCGAG GTGCCCGCTG CAATGTGATC ATCACACAAC CTCGCCGGAT CTCAGCTGTG 
TCTGTGGCAC AGCGGGTCAG CCATGAACTG GGCCCCTCCT TGCGCCGGAA TGTGGGCTTC 
CAGGTACGCT TGGAAAGTAA GCCCCCAGCC CGAGGTGGGG CGCTGCTCTT CTGCACTGTG 
GGTATCCTGC TTCGGAAGCT GCAGAGCAAC CCCAGCCTGG AGGGTGTGAG CCATGTCATT 
GTGGATGAGG TCCATGAGCG GGATGTGAAC ACAGACTTCC TGCTGATTCT GCTCAAGGGC 
CTGCAGCGGC TCAACCCAGC TCTTCGGCTG GTGCTCATGA GTGCTACAGG AGATAATGAG 
CGCTTTTCCC GATACTTTGG GGGCTGCCCT GTTATCAAGG TACCTGGCTT CATGTATCCC 
GTCAAGGAAC ACTACCTGGA GGACATCTTG GCCAAGCTGG GTAAACATCA GTACCCACAC 
CGGCATCGGC ACCATGAGTC GGAAGATGAA TGTGCACTTG ATTTGGACCT TGTGACTGAC 
CTGGTTCTGC ATATCGATGC CCGTGGGGAA CCAGGTGGGA TCCTGTGCTT TCTACCTGGC 
TGGCAAGAAA TCAAAGGAGT GCAACAACGG CTCCAGGAGG CCCTGGGCAT GCATGAAAGC 
AAGTACCTCA TCTTACCAGT GCACTCCAAT ATCCCCATGA TGGACCAGAA GGCCATATTC 
CAACAGCCTC CACTTGGGGT ACGCAAGATT GTATTGGCCA CCAACATTGC TGAAACCTCC 
ATCACAGTTA ACGACATTGT ACATGTCGTG GACAGCGGTC TGCACAAGGA GGAACGCTAT 
GACCTGAAGA CCAAGGTGTC CTGCCTGGAG ACTGTGTGGG TGTCGAGAGC AAATGTCATT 
CAGCGCCGGG GCAGGGCAGG CCGCTGCCAG TCAGGTTTTG CCTACCACTT GTTCCCGAGG 
AGCCGGCTGG AGAAAATGGT TCCTTTCCAA GTGCCAGAGA TCCTGCGCAC ACCTCTTGAG 
AACCTGGTGC TGCAAGCCAA AATCCATATG CCTGAGAAGA CGGCAGTGGA GTTCCTCTCT 
AAGGCTGTGG ACAGTCCAAA TATCAAGGCA GTGGATGAGG CCGTGATCCT GCTCCAGGAG 
ATTGGGGTGC TGGACCAGCG GGAGTATCTG ACCACCTTGG GACAGCGCCT GGCCCACATC 
TCTACTGACC CCCGACTGGC CAAGGCCATA GTGTTGGCTG CCATCTTCCG TTGCCTGCAC 
CCACTGCTGG TGGTTGTTTC CTGCCTTACC CGGGACCCCT TCAGCAGCAG TTTGCAGAAC 
CGGGCAGAAG TAGACAAGGT GAAGGCATTG CTGAGCCATG ACAGTGGCAG TGACCATTTG 
GCCTTCGTGC GGGCTGTGGC TGGCTGGGAG GAGGTACTGC GCTGGCAGGA CCGTACCTCC 
AGGGAAAACT ACCTGGAAGA AAACCTTCTG TATGCCCCCA GCTTGCGCTT CATCCACGGG 
CTCATCAAGC AGTTCTCAGA GAACATTTAT GAGGCTTTCC TAGTGGGGAA GCCCTCTGAC 
TGCACACTGC CCTCTGCTCA GTGCAATGAG TACAGCGAGG AAGAGGAGCT GGTGAAGGGT 
GTGCTGATGG CTGGCCTCTA CCCCAACCTC ATCCAGGTGA GACAAGGTAA GGTTACTCGG 
CAAGGCAAGT TCAAACCCAA CAGTGTCACT TACAGGACCA AATCTGGCAA CATCTTGCTG 
CATAAGTCAA CCATTAACAG GGAGGCTACC CGGTTACGGA GCCGATGGCT GACATATTTC 
ATGGCCGTCA AGTCCAATGG TAGCGTCTTT GTTCGAGATT CCTCCCAGGT GCACCCACTA 
GCTGTGTTGC TCCTAACAGA TGGGGACGTG CACATCCGAG ATGATGGGCG TCGGGCCACC 
ATCTCATTGA GTGACAGCGA CCTGCTTCGG CTGGAAGGTG ATTCACGAAC TGTGCGGTTG 
CTAAGGGAGT TTCGTCGAGC CCTAGGACGG ATGGTGGAGC GGAGCCTCCG CAGCGAGTTA 
GCTGCACTTC CTCTTAGTGT GCAGCAAGAA CACGGGCAGC TGCTTGCACT GCTGGCAGAG 
TTGCTTCGAG GACCTTGTGG CAGCTTTGAT ATGCGCAAGA CAGCTGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_006512310.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512373.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X12, mRNA.

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CloneID	OMu43563
Clone ID Related Accession (Same CDS sequence)	XM_006512310.3 , XM_030244646.1 , XM_030244645.1 , XM_017313615.1 , XM_006512310.4 , XM_017313616.1 , XM_030244642.1 , XM_030244643.1 , XM_030244644.1 , XM_030244647.1
Accession Version	XM_006512310.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	putative ATP-dependent RNA helicase DHX30 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512310.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512373.1
CDS	509..4069
Translation	MQASPTPSSTHVCQPCPWRDHHSRLNVNISNMAASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_006512310.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512310.3

ORF Insert Sequence:

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_017313615.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169104.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu43563
Clone ID Related Accession (Same CDS sequence)	XM_006512310.3 , XM_030244646.1 , XM_030244645.1 , XM_017313615.1 , XM_006512310.4 , XM_017313616.1 , XM_030244642.1 , XM_030244643.1 , XM_030244644.1 , XM_030244647.1
Accession Version	XM_017313615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169104.1
CDS	1028..4588
Translation	MQASPTPSSTHVCQPCPWRDHHSRLNVNISNMAASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_017313615.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313615.1

ORF Insert Sequence:

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx30 ( XM_017313616.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169105.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X20, mRNA.

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CloneID	OMu43563
Clone ID Related Accession (Same CDS sequence)	XM_006512310.3 , XM_030244646.1 , XM_030244645.1 , XM_017313615.1 , XM_006512310.4 , XM_017313616.1 , XM_030244642.1 , XM_030244643.1 , XM_030244644.1 , XM_030244647.1
Accession Version	XM_017313616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX30 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169105.1
CDS	957..4517
Translation	MQASPTPSSTHVCQPCPWRDHHSRLNVNISNMAASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD

Target ORF information:

RefSeq Version	XM_017313616.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313616.1

ORF Insert Sequence:

ATGCAAGCTT CCCCCACCCC GTCTTCCACC CATGTGTGTC AACCCTGCCC CTGGAGGGAC 
CATCACTCGA GGCTGAACGT TAACATTTCC AACATGGCAG CTTCTAGGGA CCTATTAAAA 
GAGTTTCCGC AGCCTAAAAA CCTTCTCAAC AGCGTGATTG GAAGAGCCCT TGGCATCTCA 
CATGCAAAAG ACAAGTTAGT CTATGTGCAC ACGAATGGAC CGAAGAAAAA GAAAGTCACC 
CTGCACATAA AGTGGCCCAA GAGCGTGGAG GTGGAAGGCT ATGGCAGCAA GAAGATTGAT 
GCTGAGCGTC AGGCTGCAGC AGCTGCCTGC CAACTCTTCA AGGGCTGGGG TCTGCTGGGA 
CCACGGAATG AGCTGTTTGA TGCAGCTAAA TACCGAGTGC TAGCTGATCG TTTTGGGTCT 
CCAGCTGACA GCTGGTGGCG CCCAGAACCC ACCATGCCTC CAACTTCCTG GCGGCAGCTG 
AATCCTGAGA ACATTCGGCC AGGGGGTCCT GCAGGACTAT CCCGATCCTT AGGCCGAGAG 
GAAGAGGAAG ATGAGGAGGA AGAGCTAGAA GAGGGGACCA TTGATGTGAC AGAGTTTCTG 
TCTATGACCC AGCAAGACTC CCACAACCCA CTCAGGGACT CAAGGGGGGG CTCCTTTGAA 
ATGACAGATG ATGACAGTGC TATCAGAGCT CTGACCCAGT TTCCACTTCC CAAGAACCTC 
CTGGCCAAAG TGATTCAGAT TGCAACCTCC TCCTCCACAG CTAAGAATCT CATGCAGTTC 
CATACTGTGG GTACCAAGAC CAAGCTGGCT ACACTCACTC TGCTCTGGCC CTGTCCCATG 
ACCTTTGTTG CCAAAGGGCG ACGCAAAGCT GAGGCTGAGA ATAAGGCAGC AGCCTTGGCT 
TGCAAGAAAC TGAAGAGCCT TGGCCTTGTG GACAGGAACA ATGAGCCGCT TACCCATGCC 
ATGTACAACC TGGCCTCCCT GCGTGAGTTG GGTGAGACCC AGCGCCGGCC ATGTACCATC 
CAGGTGCCTG AGCCCATCCT TCGCAAGATA GAGGCCTTCC TGAGTCATTA CCCGGTGGAC 
AGCTCATGGA TTTCCCCAGA ACTCCGACTG CAGAGTGATG ACATCTTGCC CTTAGGCAAG 
GACTCAGGGC CCTTGAGTGA CCCTATCACA GGCAAGCCAT ACATGCCCCT GTCAGAAGCA 
GAGGAGGTGC GCTTAAGCCA GAGCCTGCTA GAGCTGTGGC GGAGGAGAGG GCCGATCTGG 
CAGGAGGCCC CGCAGCTACC TGTAGACCCT CATCGGGACA CTATCCTCAG TGCCATTGAA 
CAACACCCAG TGGTAGTCAT CTCTGGGGAC ACAGGCTGTG GGAAGACCAC ACGTATCCCT 
CAGCTGCTAT TGGAGCGCTA TGTGACTGAG GGTCGAGGTG CCCGCTGCAA TGTGATCATC 
ACACAACCTC GCCGGATCTC AGCTGTGTCT GTGGCACAGC GGGTCAGCCA TGAACTGGGC 
CCCTCCTTGC GCCGGAATGT GGGCTTCCAG GTACGCTTGG AAAGTAAGCC CCCAGCCCGA 
GGTGGGGCGC TGCTCTTCTG CACTGTGGGT ATCCTGCTTC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GTGTGAGCCA TGTCATTGTG GATGAGGTCC ATGAGCGGGA TGTGAACACA 
GACTTCCTGC TGATTCTGCT CAAGGGCCTG CAGCGGCTCA ACCCAGCTCT TCGGCTGGTG 
CTCATGAGTG CTACAGGAGA TAATGAGCGC TTTTCCCGAT ACTTTGGGGG CTGCCCTGTT 
ATCAAGGTAC CTGGCTTCAT GTATCCCGTC AAGGAACACT ACCTGGAGGA CATCTTGGCC 
AAGCTGGGTA AACATCAGTA CCCACACCGG CATCGGCACC ATGAGTCGGA AGATGAATGT 
GCACTTGATT TGGACCTTGT GACTGACCTG GTTCTGCATA TCGATGCCCG TGGGGAACCA 
GGTGGGATCC TGTGCTTTCT ACCTGGCTGG CAAGAAATCA AAGGAGTGCA ACAACGGCTC 
CAGGAGGCCC TGGGCATGCA TGAAAGCAAG TACCTCATCT TACCAGTGCA CTCCAATATC 
CCCATGATGG ACCAGAAGGC CATATTCCAA CAGCCTCCAC TTGGGGTACG CAAGATTGTA 
TTGGCCACCA ACATTGCTGA AACCTCCATC ACAGTTAACG ACATTGTACA TGTCGTGGAC 
AGCGGTCTGC ACAAGGAGGA ACGCTATGAC CTGAAGACCA AGGTGTCCTG CCTGGAGACT 
GTGTGGGTGT CGAGAGCAAA TGTCATTCAG CGCCGGGGCA GGGCAGGCCG CTGCCAGTCA 
GGTTTTGCCT ACCACTTGTT CCCGAGGAGC CGGCTGGAGA AAATGGTTCC TTTCCAAGTG 
CCAGAGATCC TGCGCACACC TCTTGAGAAC CTGGTGCTGC AAGCCAAAAT CCATATGCCT 
GAGAAGACGG CAGTGGAGTT CCTCTCTAAG GCTGTGGACA GTCCAAATAT CAAGGCAGTG 
GATGAGGCCG TGATCCTGCT CCAGGAGATT GGGGTGCTGG ACCAGCGGGA GTATCTGACC 
ACCTTGGGAC AGCGCCTGGC CCACATCTCT ACTGACCCCC GACTGGCCAA GGCCATAGTG 
TTGGCTGCCA TCTTCCGTTG CCTGCACCCA CTGCTGGTGG TTGTTTCCTG CCTTACCCGG 
GACCCCTTCA GCAGCAGTTT GCAGAACCGG GCAGAAGTAG ACAAGGTGAA GGCATTGCTG 
AGCCATGACA GTGGCAGTGA CCATTTGGCC TTCGTGCGGG CTGTGGCTGG CTGGGAGGAG 
GTACTGCGCT GGCAGGACCG TACCTCCAGG GAAAACTACC TGGAAGAAAA CCTTCTGTAT 
GCCCCCAGCT TGCGCTTCAT CCACGGGCTC ATCAAGCAGT TCTCAGAGAA CATTTATGAG 
GCTTTCCTAG TGGGGAAGCC CTCTGACTGC ACACTGCCCT CTGCTCAGTG CAATGAGTAC 
AGCGAGGAAG AGGAGCTGGT GAAGGGTGTG CTGATGGCTG GCCTCTACCC CAACCTCATC 
CAGGTGAGAC AAGGTAAGGT TACTCGGCAA GGCAAGTTCA AACCCAACAG TGTCACTTAC 
AGGACCAAAT CTGGCAACAT CTTGCTGCAT AAGTCAACCA TTAACAGGGA GGCTACCCGG 
TTACGGAGCC GATGGCTGAC ATATTTCATG GCCGTCAAGT CCAATGGTAG CGTCTTTGTT 
CGAGATTCCT CCCAGGTGCA CCCACTAGCT GTGTTGCTCC TAACAGATGG GGACGTGCAC 
ATCCGAGATG ATGGGCGTCG GGCCACCATC TCATTGAGTG ACAGCGACCT GCTTCGGCTG 
GAAGGTGATT CACGAACTGT GCGGTTGCTA AGGGAGTTTC GTCGAGCCCT AGGACGGATG 
GTGGAGCGGA GCCTCCGCAG CGAGTTAGCT GCACTTCCTC TTAGTGTGCA GCAAGAACAC 
GGGCAGCTGC TTGCACTGCT GGCAGAGTTG CTTCGAGGAC CTTGTGGCAG CTTTGATATG 
CGCAAGACAG CTGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The novel helicase helG (DHX30) is expressed during gastrulation in mice and has a structure similar to a human DExH box helicase.
Stem cells and development24(3)372-83(2015 Feb)
Zheng HJ,Tsukahara M,Liu E,Ye L,Xiong H,Noguchi S,Suzuki K,Ji ZS

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Raftlin is involved in the nucleocapture complex to induce poly(I:C)-mediated TLR3 activation.
The Journal of biological chemistry286(12)10702-11(2011 Mar)
Watanabe A,Tatematsu M,Saeki K,Shibata S,Shime H,Yoshimura A,Obuse C,Seya T,Matsumoto M

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Dhx30 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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