F12 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	F12
Entrez Gene ID	2161
Full Name	coagulation factor XII
Synonyms	HAE3,HAEX,HAF
General protein information	Preferred Names coagulation factor XII Names coagulation factor XII Hageman factor beta-factor XIIa part 1 beta-factor XIIa part 2 coagulation factor XIIa heavy chain coagulation factor XIIa light chain
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q35.3
Summary	This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq, Jul 2008].
Disorder MIM:	610619
Disorder Html:	Factor XII deficiency, 234000 (3); Angioedema, hereditary, type III, 610618 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000505.3	NP_000496.2	coagulation factor XII preproprotein
XM_011534461.1	XP_011532763.1	coagulation factor XII isoform X1
XM_011534462.2	XP_011532764.1	coagulation factor XII isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	F12	XP_518130.2
Xenopus tropicalis (tropical clawed frog)	f12	XP_002937000.1
Homo sapiens (human)	F12	NP_000496.2
	F12	XP_001089771.1
Canis lupus familiaris (dog)	F12	XP_005619216.1
Rattus norvegicus (Norway rat)	F12	NP_001014028.1
Bos taurus (cattle)	F12	NP_001068587.1
Mus musculus (house mouse)	F12	NP_067464.2

Gene Ontology
Bibliography

Related articles in PubMed

Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans.
Calvo SE, Pagliarini DJ, Mootha VK
Proceedings of the National Academy of Sciences of the United States of America106(18)7507-12(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

beta-amyloid interacts with fibrinogen and factor XII. These interactions can lead to increased clotting, abnormal clot formation, persistent fibrin deposition, and generation of proinflammatory molecules.
Title: Interactions of ?-amyloid peptide with fibrinogen and coagulation factor XII may contribute to Alzheimer's disease.

inhibition of FXI and FXII distinctly alter the biophysical properties of fibrin.
Title: Differential Roles for the Coagulation Factors XI and XII in Regulating the Physical Biology of Fibrin.

results support a model for induction of contact activation in which activity intrinsic to single-chain FXII initiates alphaFXIIa and alpha-kallikrein formation on a surface. alphaFXIIa, with support from alpha-kallikrein, subsequently accelerates contact activation and is responsible for the full procoagulant activity of FXII.
Title: Proteolytic properties of single-chain factor XII: a mechanism for triggering contact activation.

Report an independent association between FXII levels and the risk of hemorrhagic stroke in Swedish population.
Title: Factor XII as a Risk Marker for Hemorrhagic Stroke: A Prospective Cohort Study.

The XPNPEP2 c-2399A and the ACE insertion/deletion polymorphisms analyzed in a population of patients with hereditary angioedema with F12 mutation were not a major determinant of disease expression.
Title: Hereditary angioedema with F12 mutation: Clinical features and enzyme polymorphisms in 9 Southwestern Spanish families.

The following F12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23884	NM_000505.3 Latest version!	Homo sapiens coagulation factor XII (F12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu23884	XM_011534461.1 Latest version!	Homo sapiens coagulation factor XII (Hageman factor) (F12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu57667	XM_011534462.2 Latest version!	Homo sapiens coagulation factor XII (F12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F12 ( NM_000505.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000496.2 Homo sapiens coagulation factor XII (F12), mRNA.

Price & Availability↑

CloneID	OHu23884
Clone ID Related Accession (Same CDS sequence)	NM_000505.3 , XM_011534461.1
Accession Version	NM_000505.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	coagulation factor XII preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC145098.2, AB095845.1, M11723.1 and BE139501.1. This sequence is a reference standard in the RefSeqGene project. On Apr 13, 2007 this sequence version replaced NM_000505.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB095845.1, M31315.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein regulatory uORF :: PMID: 19372376 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGGCTC TGCTGCTCCT GGGGTTCCTG CTGGTGAGCT TGGAGTCAAC ACTTTCGATT 
CCACCTTGGG AAGCCCCCAA GGAGCATAAG TACAAAGCTG AAGAGCACAC AGTCGTTCTC 
ACTGTCACCG GGGAGCCCTG CCACTTCCCC TTCCAGTACC ACCGGCAGCT GTACCACAAA 
TGTACCCACA AGGGCCGGCC AGGCCCTCAG CCCTGGTGTG CTACCACCCC CAACTTTGAT 
CAGGACCAGC GATGGGGATA CTGTTTGGAG CCCAAGAAAG TGAAAGACCA CTGCAGCAAA 
CACAGCCCCT GCCAGAAAGG AGGGACCTGT GTGAACATGC CAAGCGGCCC CCACTGTCTC 
TGTCCACAAC ACCTCACTGG AAACCACTGC CAGAAAGAGA AGTGCTTTGA GCCTCAGCTT 
CTCCGGTTTT TCCACAAGAA TGAGATATGG TATAGAACTG AGCAAGCAGC TGTGGCCAGA 
TGCCAGTGCA AGGGTCCTGA TGCCCACTGC CAGCGGCTGG CCAGCCAGGC CTGCCGCACC 
AACCCGTGCC TCCATGGGGG TCGCTGCCTA GAGGTGGAGG GCCACCGCCT GTGCCACTGC 
CCGGTGGGCT ACACCGGAGC CTTCTGCGAC GTGGACACCA AGGCAAGCTG CTATGATGGC 
CGCGGGCTCA GCTACCGCGG CCTGGCCAGG ACCACGCTCT CGGGTGCGCC CTGTCAGCCG 
TGGGCCTCGG AGGCCACCTA CCGGAACGTG ACTGCCGAGC AAGCGCGGAA CTGGGGACTG 
GGCGGCCACG CCTTCTGCCG GAACCCGGAC AACGACATCC GCCCGTGGTG CTTCGTGCTG 
AACCGCGACC GGCTGAGCTG GGAGTACTGC GACCTGGCAC AGTGCCAGAC CCCAACCCAG 
GCGGCGCCTC CGACCCCGGT GTCCCCTAGG CTTCATGTCC CACTCATGCC CGCGCAGCCG 
GCACCGCCGA AGCCTCAGCC CACGACCCGG ACCCCGCCTC AGTCCCAGAC CCCGGGAGCC 
TTGCCGGCGA AGCGGGAGCA GCCGCCTTCC CTGACCAGGA ACGGCCCACT GAGCTGCGGG 
CAGCGGCTCC GCAAGAGTCT GTCTTCGATG ACCCGCGTCG TTGGCGGGCT GGTGGCGCTA 
CGCGGGGCGC ACCCCTACAT CGCCGCGCTG TACTGGGGCC ACAGTTTCTG CGCCGGCAGC 
CTCATCGCCC CCTGCTGGGT GCTGACGGCC GCTCACTGCC TGCAGGACCG GCCCGCACCC 
GAGGATCTGA CGGTGGTGCT CGGCCAGGAA CGCCGTAACC ACAGCTGTGA GCCGTGCCAG 
ACGTTGGCCG TGCGCTCCTA CCGCTTGCAC GAGGCCTTCT CGCCCGTCAG CTACCAGCAC 
GACCTGGCTC TGTTGCGCCT TCAGGAGGAT GCGGACGGCA GCTGCGCGCT CCTGTCGCCT 
TACGTTCAGC CGGTGTGCCT GCCAAGCGGC GCCGCGCGAC CCTCCGAGAC CACGCTCTGC 
CAGGTGGCCG GCTGGGGCCA CCAGTTCGAG GGGGCGGAGG AATATGCCAG CTTCCTGCAG 
GAGGCGCAGG TACCGTTCCT CTCCCTGGAG CGCTGCTCAG CCCCGGACGT GCACGGATCC 
TCCATCCTCC CCGGCATGCT CTGCGCAGGG TTCCTCGAGG GCGGCACCGA TGCGTGCCAG 
GGTGATTCCG GAGGCCCGCT GGTGTGTGAG GACCAAGCTG CAGAGCGCCG GCTCACCCTG 
CAAGGCATCA TCAGCTGGGG ATCGGGCTGT GGTGACCGCA ACAAGCCAGG CGTCTACACC 
GATGTGGCCT ACTACCTGGC CTGGATCCGG GAGCACACCG TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000496.2
CDS	50..1897
Translation	MRALLLLGFLLVSLESTLSIPPWEAPKEHKYKAEEHTVVLTVTGEPCHFPFQYHRQLYHKCTHKGRPGPQPWCATTPNFDQDQRWGYCLEPKKVKDHCSKHSPCQKGGTCVNMPSGPHCLCPQHLTGNHCQKEKCFEPQLLRFFHKNEIWYRTEQAAVARCQCKGPDAHCQRLASQACRTNPCLHGGRCLEVEGHRLCHCPVGYTGAFCDVDTKASCYDGRGLSYRGLARTTLSGAPCQPWASEATYRNVTAEQARNWGLGGHAFCRNPDNDIRPWCFVLNRDRLSWEYCDLAQCQTPTQAAPPTPVSPRLHVPLMPAQPAPPKPQPTTRTPPQSQTPGALPAKREQPPSLTRNGPLSCGQRLRKSLSSMTRVVGGLVALRGAHPYIAALYWGHSFCAGSLIAPCWVLTAAHCLQDRPAPEDLTVVLGQERRNHSCEPCQTLAVRSYRLHEAFSPVSYQHDLALLRLQEDADGSCALLSPYVQPVCLPSGAARPSETTLCQVAGWGHQFEGAEEYASFLQEAQVPFLSLERCSAPDVHGSSILPGMLCAGFLEGGTDACQGDSGGPLVCEDQAAERRLTLQGIISWGSGCGDRNKPGVYTDVAYYLAWIREHTVS

Target ORF information:

RefSeq Version	NM_000505.3
Organism	Homo sapiens(human)
Definition	Homo sapiens coagulation factor XII (F12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000505.3

ORF Insert Sequence:

ATGAGGGCTC TGCTGCTCCT GGGGTTCCTG CTGGTGAGCT TGGAGTCAAC ACTTTCGATT 
CCACCTTGGG AAGCCCCCAA GGAGCATAAG TACAAAGCTG AAGAGCACAC AGTCGTTCTC 
ACTGTCACCG GGGAGCCCTG CCACTTCCCC TTCCAGTACC ACCGGCAGCT GTACCACAAA 
TGTACCCACA AGGGCCGGCC AGGCCCTCAG CCCTGGTGTG CTACCACCCC CAACTTTGAT 
CAGGACCAGC GATGGGGATA CTGTTTGGAG CCCAAGAAAG TGAAAGACCA CTGCAGCAAA 
CACAGCCCCT GCCAGAAAGG AGGGACCTGT GTGAACATGC CAAGCGGCCC CCACTGTCTC 
TGTCCACAAC ACCTCACTGG AAACCACTGC CAGAAAGAGA AGTGCTTTGA GCCTCAGCTT 
CTCCGGTTTT TCCACAAGAA TGAGATATGG TATAGAACTG AGCAAGCAGC TGTGGCCAGA 
TGCCAGTGCA AGGGTCCTGA TGCCCACTGC CAGCGGCTGG CCAGCCAGGC CTGCCGCACC 
AACCCGTGCC TCCATGGGGG TCGCTGCCTA GAGGTGGAGG GCCACCGCCT GTGCCACTGC 
CCGGTGGGCT ACACCGGAGC CTTCTGCGAC GTGGACACCA AGGCAAGCTG CTATGATGGC 
CGCGGGCTCA GCTACCGCGG CCTGGCCAGG ACCACGCTCT CGGGTGCGCC CTGTCAGCCG 
TGGGCCTCGG AGGCCACCTA CCGGAACGTG ACTGCCGAGC AAGCGCGGAA CTGGGGACTG 
GGCGGCCACG CCTTCTGCCG GAACCCGGAC AACGACATCC GCCCGTGGTG CTTCGTGCTG 
AACCGCGACC GGCTGAGCTG GGAGTACTGC GACCTGGCAC AGTGCCAGAC CCCAACCCAG 
GCGGCGCCTC CGACCCCGGT GTCCCCTAGG CTTCATGTCC CACTCATGCC CGCGCAGCCG 
GCACCGCCGA AGCCTCAGCC CACGACCCGG ACCCCGCCTC AGTCCCAGAC CCCGGGAGCC 
TTGCCGGCGA AGCGGGAGCA GCCGCCTTCC CTGACCAGGA ACGGCCCACT GAGCTGCGGG 
CAGCGGCTCC GCAAGAGTCT GTCTTCGATG ACCCGCGTCG TTGGCGGGCT GGTGGCGCTA 
CGCGGGGCGC ACCCCTACAT CGCCGCGCTG TACTGGGGCC ACAGTTTCTG CGCCGGCAGC 
CTCATCGCCC CCTGCTGGGT GCTGACGGCC GCTCACTGCC TGCAGGACCG GCCCGCACCC 
GAGGATCTGA CGGTGGTGCT CGGCCAGGAA CGCCGTAACC ACAGCTGTGA GCCGTGCCAG 
ACGTTGGCCG TGCGCTCCTA CCGCTTGCAC GAGGCCTTCT CGCCCGTCAG CTACCAGCAC 
GACCTGGCTC TGTTGCGCCT TCAGGAGGAT GCGGACGGCA GCTGCGCGCT CCTGTCGCCT 
TACGTTCAGC CGGTGTGCCT GCCAAGCGGC GCCGCGCGAC CCTCCGAGAC CACGCTCTGC 
CAGGTGGCCG GCTGGGGCCA CCAGTTCGAG GGGGCGGAGG AATATGCCAG CTTCCTGCAG 
GAGGCGCAGG TACCGTTCCT CTCCCTGGAG CGCTGCTCAG CCCCGGACGT GCACGGATCC 
TCCATCCTCC CCGGCATGCT CTGCGCAGGG TTCCTCGAGG GCGGCACCGA TGCGTGCCAG 
GGTGATTCCG GAGGCCCGCT GGTGTGTGAG GACCAAGCTG CAGAGCGCCG GCTCACCCTG 
CAAGGCATCA TCAGCTGGGG ATCGGGCTGT GGTGACCGCA ACAAGCCAGG CGTCTACACC 
GATGTGGCCT ACTACCTGGC CTGGATCCGG GAGCACACCG TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F12 ( XM_011534461.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532763.1 Homo sapiens coagulation factor XII (Hageman factor) (F12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu23884
Clone ID Related Accession (Same CDS sequence)	NM_000505.3 , XM_011534461.1
Accession Version	XM_011534461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	coagulation factor XII isoform X1
Comment

ATGAGGGCTC TGCTGCTCCT GGGGTTCCTG CTGGTGAGCT TGGAGTCAAC ACTTTCGATT 
CCACCTTGGG AAGCCCCCAA GGAGCATAAG TACAAAGCTG AAGAGCACAC AGTCGTTCTC 
ACTGTCACCG GGGAGCCCTG CCACTTCCCC TTCCAGTACC ACCGGCAGCT GTACCACAAA 
TGTACCCACA AGGGCCGGCC AGGCCCTCAG CCCTGGTGTG CTACCACCCC CAACTTTGAT 
CAGGACCAGC GATGGGGATA CTGTTTGGAG CCCAAGAAAG TGAAAGACCA CTGCAGCAAA 
CACAGCCCCT GCCAGAAAGG AGGGACCTGT GTGAACATGC CAAGCGGCCC CCACTGTCTC 
TGTCCACAAC ACCTCACTGG AAACCACTGC CAGAAAGAGA AGTGCTTTGA GCCTCAGCTT 
CTCCGGTTTT TCCACAAGAA TGAGATATGG TATAGAACTG AGCAAGCAGC TGTGGCCAGA 
TGCCAGTGCA AGGGTCCTGA TGCCCACTGC CAGCGGCTGG CCAGCCAGGC CTGCCGCACC 
AACCCGTGCC TCCATGGGGG TCGCTGCCTA GAGGTGGAGG GCCACCGCCT GTGCCACTGC 
CCGGTGGGCT ACACCGGAGC CTTCTGCGAC GTGGACACCA AGGCAAGCTG CTATGATGGC 
CGCGGGCTCA GCTACCGCGG CCTGGCCAGG ACCACGCTCT CGGGTGCGCC CTGTCAGCCG 
TGGGCCTCGG AGGCCACCTA CCGGAACGTG ACTGCCGAGC AAGCGCGGAA CTGGGGACTG 
GGCGGCCACG CCTTCTGCCG GAACCCGGAC AACGACATCC GCCCGTGGTG CTTCGTGCTG 
AACCGCGACC GGCTGAGCTG GGAGTACTGC GACCTGGCAC AGTGCCAGAC CCCAACCCAG 
GCGGCGCCTC CGACCCCGGT GTCCCCTAGG CTTCATGTCC CACTCATGCC CGCGCAGCCG 
GCACCGCCGA AGCCTCAGCC CACGACCCGG ACCCCGCCTC AGTCCCAGAC CCCGGGAGCC 
TTGCCGGCGA AGCGGGAGCA GCCGCCTTCC CTGACCAGGA ACGGCCCACT GAGCTGCGGG 
CAGCGGCTCC GCAAGAGTCT GTCTTCGATG ACCCGCGTCG TTGGCGGGCT GGTGGCGCTA 
CGCGGGGCGC ACCCCTACAT CGCCGCGCTG TACTGGGGCC ACAGTTTCTG CGCCGGCAGC 
CTCATCGCCC CCTGCTGGGT GCTGACGGCC GCTCACTGCC TGCAGGACCG GCCCGCACCC 
GAGGATCTGA CGGTGGTGCT CGGCCAGGAA CGCCGTAACC ACAGCTGTGA GCCGTGCCAG 
ACGTTGGCCG TGCGCTCCTA CCGCTTGCAC GAGGCCTTCT CGCCCGTCAG CTACCAGCAC 
GACCTGGCTC TGTTGCGCCT TCAGGAGGAT GCGGACGGCA GCTGCGCGCT CCTGTCGCCT 
TACGTTCAGC CGGTGTGCCT GCCAAGCGGC GCCGCGCGAC CCTCCGAGAC CACGCTCTGC 
CAGGTGGCCG GCTGGGGCCA CCAGTTCGAG GGGGCGGAGG AATATGCCAG CTTCCTGCAG 
GAGGCGCAGG TACCGTTCCT CTCCCTGGAG CGCTGCTCAG CCCCGGACGT GCACGGATCC 
TCCATCCTCC CCGGCATGCT CTGCGCAGGG TTCCTCGAGG GCGGCACCGA TGCGTGCCAG 
GGTGATTCCG GAGGCCCGCT GGTGTGTGAG GACCAAGCTG CAGAGCGCCG GCTCACCCTG 
CAAGGCATCA TCAGCTGGGG ATCGGGCTGT GGTGACCGCA ACAAGCCAGG CGTCTACACC 
GATGTGGCCT ACTACCTGGC CTGGATCCGG GAGCACACCG TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011532763.1

Translation

MRALLLLGFLLVSLESTLSIPPWEAPKEHKYKAEEHTVVLTVTGEPCHFPFQYHRQLYHKCTHKGRPGPQPWCATTPNFDQDQRWGYCLEPKKVKDHCSKHSPCQKGGTCVNMPSGPHCLCPQHLTGNHCQKEKCFEPQLLRFFHKNEIWYRTEQAAVARCQCKGPDAHCQRLASQACRTNPCLHGGRCLEVEGHRLCHCPVGYTGAFCDVDTKASCYDGRGLSYRGLARTTLSGAPCQPWASEATYRNVTAEQARNWGLGGHAFCRNPDNDIRPWCFVLNRDRLSWEYCDLAQCQTPTQAAPPTPVSPRLHVPLMPAQPAPPKPQPTTRTPPQSQTPGALPAKREQPPSLTRNGPLSCGQRLRKSLSSMTRVVGGLVALRGAHPYIAALYWGHSFCAGSLIAPCWVLTAAHCLQDRPAPEDLTVVLGQERRNHSCEPCQTLAVRSYRLHEAFSPVSYQHDLALLRLQEDADGSCALLSPYVQPVCLPSGAARPSETTLCQVAGWGHQFEGAEEYASFLQEAQVPFLSLERCSAPDVHGSSILPGMLCAGFLEGGTDACQGDSGGPLVCEDQAAERRLTLQGIISWGSGCGDRNKPGVYTDVAYYLAWIREHTVS

Target ORF information:

RefSeq Version	XM_011534461.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens coagulation factor XII (Hageman factor) (F12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534461.1

ORF Insert Sequence:

ATGAGGGCTC TGCTGCTCCT GGGGTTCCTG CTGGTGAGCT TGGAGTCAAC ACTTTCGATT 
CCACCTTGGG AAGCCCCCAA GGAGCATAAG TACAAAGCTG AAGAGCACAC AGTCGTTCTC 
ACTGTCACCG GGGAGCCCTG CCACTTCCCC TTCCAGTACC ACCGGCAGCT GTACCACAAA 
TGTACCCACA AGGGCCGGCC AGGCCCTCAG CCCTGGTGTG CTACCACCCC CAACTTTGAT 
CAGGACCAGC GATGGGGATA CTGTTTGGAG CCCAAGAAAG TGAAAGACCA CTGCAGCAAA 
CACAGCCCCT GCCAGAAAGG AGGGACCTGT GTGAACATGC CAAGCGGCCC CCACTGTCTC 
TGTCCACAAC ACCTCACTGG AAACCACTGC CAGAAAGAGA AGTGCTTTGA GCCTCAGCTT 
CTCCGGTTTT TCCACAAGAA TGAGATATGG TATAGAACTG AGCAAGCAGC TGTGGCCAGA 
TGCCAGTGCA AGGGTCCTGA TGCCCACTGC CAGCGGCTGG CCAGCCAGGC CTGCCGCACC 
AACCCGTGCC TCCATGGGGG TCGCTGCCTA GAGGTGGAGG GCCACCGCCT GTGCCACTGC 
CCGGTGGGCT ACACCGGAGC CTTCTGCGAC GTGGACACCA AGGCAAGCTG CTATGATGGC 
CGCGGGCTCA GCTACCGCGG CCTGGCCAGG ACCACGCTCT CGGGTGCGCC CTGTCAGCCG 
TGGGCCTCGG AGGCCACCTA CCGGAACGTG ACTGCCGAGC AAGCGCGGAA CTGGGGACTG 
GGCGGCCACG CCTTCTGCCG GAACCCGGAC AACGACATCC GCCCGTGGTG CTTCGTGCTG 
AACCGCGACC GGCTGAGCTG GGAGTACTGC GACCTGGCAC AGTGCCAGAC CCCAACCCAG 
GCGGCGCCTC CGACCCCGGT GTCCCCTAGG CTTCATGTCC CACTCATGCC CGCGCAGCCG 
GCACCGCCGA AGCCTCAGCC CACGACCCGG ACCCCGCCTC AGTCCCAGAC CCCGGGAGCC 
TTGCCGGCGA AGCGGGAGCA GCCGCCTTCC CTGACCAGGA ACGGCCCACT GAGCTGCGGG 
CAGCGGCTCC GCAAGAGTCT GTCTTCGATG ACCCGCGTCG TTGGCGGGCT GGTGGCGCTA 
CGCGGGGCGC ACCCCTACAT CGCCGCGCTG TACTGGGGCC ACAGTTTCTG CGCCGGCAGC 
CTCATCGCCC CCTGCTGGGT GCTGACGGCC GCTCACTGCC TGCAGGACCG GCCCGCACCC 
GAGGATCTGA CGGTGGTGCT CGGCCAGGAA CGCCGTAACC ACAGCTGTGA GCCGTGCCAG 
ACGTTGGCCG TGCGCTCCTA CCGCTTGCAC GAGGCCTTCT CGCCCGTCAG CTACCAGCAC 
GACCTGGCTC TGTTGCGCCT TCAGGAGGAT GCGGACGGCA GCTGCGCGCT CCTGTCGCCT 
TACGTTCAGC CGGTGTGCCT GCCAAGCGGC GCCGCGCGAC CCTCCGAGAC CACGCTCTGC 
CAGGTGGCCG GCTGGGGCCA CCAGTTCGAG GGGGCGGAGG AATATGCCAG CTTCCTGCAG 
GAGGCGCAGG TACCGTTCCT CTCCCTGGAG CGCTGCTCAG CCCCGGACGT GCACGGATCC 
TCCATCCTCC CCGGCATGCT CTGCGCAGGG TTCCTCGAGG GCGGCACCGA TGCGTGCCAG 
GGTGATTCCG GAGGCCCGCT GGTGTGTGAG GACCAAGCTG CAGAGCGCCG GCTCACCCTG 
CAAGGCATCA TCAGCTGGGG ATCGGGCTGT GGTGACCGCA ACAAGCCAGG CGTCTACACC 
GATGTGGCCT ACTACCTGGC CTGGATCCGG GAGCACACCG TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F12 ( XM_011534462.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532764.1 Homo sapiens coagulation factor XII (F12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu57667
Clone ID Related Accession (Same CDS sequence)	XM_011534462.2
Accession Version	XM_011534462.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	coagulation factor XII isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534462.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGCG GCCCCCACTG TCTCTGTCCA CAACACCTCA CTGGAAACCA CTGCCAGAAA 
GAGAAGTGCT TTGAGCCTCA GCTTCTCCGG TTTTTCCACA AGAATGAGAT ATGGTATAGA 
ACTGAGCAAG CAGCTGTGGC CAGATGCCAG TGCAAGGGTC CTGATGCCCA CTGCCAGCGG 
CTGGCCAGCC AGGCCTGCCG CACCAACCCG TGCCTCCATG GGGGTCGCTG CCTAGAGGTG 
GAGGGCCACC GCCTGTGCCA CTGCCCGGTG GGCTACACCG GAGCCTTCTG CGACGTGGAC 
ACCAAGGCAA GCTGCTATGA TGGCCGCGGG CTCAGCTACC GCGGCCTGGC CAGGACCACG 
CTCTCGGGTG CGCCCTGTCA GCCGTGGGCC TCGGAGGCCA CCTACCGGAA CGTGACTGCC 
GAGCAAGCGC GGAACTGGGG ACTGGGCGGC CACGCCTTCT GCCGGAACCC GGACAACGAC 
ATCCGCCCGT GGTGCTTCGT GCTGAACCGC GACCGGCTGA GCTGGGAGTA CTGCGACCTG 
GCACAGTGCC AGACCCCAAC CCAGGCGGCG CCTCCGACCC CGGTGTCCCC TAGGCTTCAT 
GTCCCACTCA TGCCCGCGCA GCCGGCACCG CCGAAGCCTC AGCCCACGAC CCGGACCCCG 
CCTCAGTCCC AGACCCCGGG AGCCTTGCCG GCGAAGCGGG AGCAGCCGCC TTCCCTGACC 
AGGAACGGCC CACTGAGCTG CGGGCAGCGG CTCCGCAAGA GTCTGTCTTC GATGACCCGC 
GTCGTTGGCG GGCTGGTGGC GCTACGCGGG GCGCACCCCT ACATCGCCGC GCTGTACTGG 
GGCCACAGTT TCTGCGCCGG CAGCCTCATC GCCCCCTGCT GGGTGCTGAC GGCCGCTCAC 
TGCCTGCAGG ACCGGCCCGC ACCCGAGGAT CTGACGGTGG TGCTCGGCCA GGAACGCCGT 
AACCACAGCT GTGAGCCGTG CCAGACGTTG GCCGTGCGCT CCTACCGCTT GCACGAGGCC 
TTCTCGCCCG TCAGCTACCA GCACGACCTG GCTCTGTTGC GCCTTCAGGA GGATGCGGAC 
GGCAGCTGCG CGCTCCTGTC GCCTTACGTT CAGCCGGTGT GCCTGCCAAG CGGCGCCGCG 
CGACCCTCCG AGACCACGCT CTGCCAGGTG GCCGGCTGGG GCCACCAGTT CGAGGGGGCG 
GAGGAATATG CCAGCTTCCT GCAGGAGGCG CAGGTACCGT TCCTCTCCCT GGAGCGCTGC 
TCAGCCCCGG ACGTGCACGG ATCCTCCATC CTCCCCGGCA TGCTCTGCGC AGGGTTCCTC 
GAGGGCGGCA CCGATGCGTG CCAGGGTGAT TCCGGAGGCC CGCTGGTGTG TGAGGACCAA 
GCTGCAGAGC GCCGGCTCAC CCTGCAAGGC ATCATCAGCT GGGGATCGGG CTGTGGTGAC 
CGCAACAAGC CAGGCGTCTA CACCGATGTG GCCTACTACC TGGCCTGGAT CCGGGAGCAC 
ACCGTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532764.1
CDS	220..1731
Translation	MPSGPHCLCPQHLTGNHCQKEKCFEPQLLRFFHKNEIWYRTEQAAVARCQCKGPDAHCQRLASQACRTNPCLHGGRCLEVEGHRLCHCPVGYTGAFCDVDTKASCYDGRGLSYRGLARTTLSGAPCQPWASEATYRNVTAEQARNWGLGGHAFCRNPDNDIRPWCFVLNRDRLSWEYCDLAQCQTPTQAAPPTPVSPRLHVPLMPAQPAPPKPQPTTRTPPQSQTPGALPAKREQPPSLTRNGPLSCGQRLRKSLSSMTRVVGGLVALRGAHPYIAALYWGHSFCAGSLIAPCWVLTAAHCLQDRPAPEDLTVVLGQERRNHSCEPCQTLAVRSYRLHEAFSPVSYQHDLALLRLQEDADGSCALLSPYVQPVCLPSGAARPSETTLCQVAGWGHQFEGAEEYASFLQEAQVPFLSLERCSAPDVHGSSILPGMLCAGFLEGGTDACQGDSGGPLVCEDQAAERRLTLQGIISWGSGCGDRNKPGVYTDVAYYLAWIREHTVS

Target ORF information:

RefSeq Version	XM_011534462.2
Organism	Homo sapiens(human)
Definition	Homo sapiens coagulation factor XII (F12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534462.2

ORF Insert Sequence:

ATGCCAAGCG GCCCCCACTG TCTCTGTCCA CAACACCTCA CTGGAAACCA CTGCCAGAAA 
GAGAAGTGCT TTGAGCCTCA GCTTCTCCGG TTTTTCCACA AGAATGAGAT ATGGTATAGA 
ACTGAGCAAG CAGCTGTGGC CAGATGCCAG TGCAAGGGTC CTGATGCCCA CTGCCAGCGG 
CTGGCCAGCC AGGCCTGCCG CACCAACCCG TGCCTCCATG GGGGTCGCTG CCTAGAGGTG 
GAGGGCCACC GCCTGTGCCA CTGCCCGGTG GGCTACACCG GAGCCTTCTG CGACGTGGAC 
ACCAAGGCAA GCTGCTATGA TGGCCGCGGG CTCAGCTACC GCGGCCTGGC CAGGACCACG 
CTCTCGGGTG CGCCCTGTCA GCCGTGGGCC TCGGAGGCCA CCTACCGGAA CGTGACTGCC 
GAGCAAGCGC GGAACTGGGG ACTGGGCGGC CACGCCTTCT GCCGGAACCC GGACAACGAC 
ATCCGCCCGT GGTGCTTCGT GCTGAACCGC GACCGGCTGA GCTGGGAGTA CTGCGACCTG 
GCACAGTGCC AGACCCCAAC CCAGGCGGCG CCTCCGACCC CGGTGTCCCC TAGGCTTCAT 
GTCCCACTCA TGCCCGCGCA GCCGGCACCG CCGAAGCCTC AGCCCACGAC CCGGACCCCG 
CCTCAGTCCC AGACCCCGGG AGCCTTGCCG GCGAAGCGGG AGCAGCCGCC TTCCCTGACC 
AGGAACGGCC CACTGAGCTG CGGGCAGCGG CTCCGCAAGA GTCTGTCTTC GATGACCCGC 
GTCGTTGGCG GGCTGGTGGC GCTACGCGGG GCGCACCCCT ACATCGCCGC GCTGTACTGG 
GGCCACAGTT TCTGCGCCGG CAGCCTCATC GCCCCCTGCT GGGTGCTGAC GGCCGCTCAC 
TGCCTGCAGG ACCGGCCCGC ACCCGAGGAT CTGACGGTGG TGCTCGGCCA GGAACGCCGT 
AACCACAGCT GTGAGCCGTG CCAGACGTTG GCCGTGCGCT CCTACCGCTT GCACGAGGCC 
TTCTCGCCCG TCAGCTACCA GCACGACCTG GCTCTGTTGC GCCTTCAGGA GGATGCGGAC 
GGCAGCTGCG CGCTCCTGTC GCCTTACGTT CAGCCGGTGT GCCTGCCAAG CGGCGCCGCG 
CGACCCTCCG AGACCACGCT CTGCCAGGTG GCCGGCTGGG GCCACCAGTT CGAGGGGGCG 
GAGGAATATG CCAGCTTCCT GCAGGAGGCG CAGGTACCGT TCCTCTCCCT GGAGCGCTGC 
TCAGCCCCGG ACGTGCACGG ATCCTCCATC CTCCCCGGCA TGCTCTGCGC AGGGTTCCTC 
GAGGGCGGCA CCGATGCGTG CCAGGGTGAT TCCGGAGGCC CGCTGGTGTG TGAGGACCAA 
GCTGCAGAGC GCCGGCTCAC CCTGCAAGGC ATCATCAGCT GGGGATCGGG CTGTGGTGAC 
CGCAACAAGC CAGGCGTCTA CACCGATGTG GCCTACTACC TGGCCTGGAT CCGGGAGCAC 
ACCGTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans.
Proceedings of the National Academy of Sciences of the United States of America106(18)7507-12(2009 May)
Calvo SE,Pagliarini DJ,Mootha VK

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F12 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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