ENO1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001428.4	NP_001419.1	alpha-enolase isoform 1
NM_001428.3	NP_001419.1	alpha-enolase isoform 1
NM_001201483.1	NP_001188412.1	c-myc promoter-binding protein-1 isoform MBP-1
NM_001353346.2	NP_001340275.1	alpha-enolase isoform 3
NM_001201483.3	NP_001188412.1	c-myc promoter-binding protein-1 isoform MBP-1
NM_001353346.3	NP_001340275.1	alpha-enolase isoform 3
NM_001201483.4	NP_001188412.1	c-myc promoter-binding protein-1 isoform MBP-1
XM_006710433.2	XP_006710496.1	alpha-enolase isoform X1
NM_001428.5	NP_001419.1	alpha-enolase isoform 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17630	NM_001201483.4 Latest version!	Homo sapiens enolase 1 (ENO1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu19186	NM_001428.5 Latest version!	Homo sapiens enolase 1 (ENO1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu33139	NM_001353346.3 Latest version!	Homo sapiens enolase 1 (ENO1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu33139	XM_006710433.2 Latest version!	Homo sapiens enolase 1 (ENO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu19186	NM_001428.3	Homo sapiens enolase 1 (ENO1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu17630	NM_001201483.1	Homo sapiens enolase 1 (ENO1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu33139	NM_001353346.2	Homo sapiens enolase 1 (ENO1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu17630	NM_001201483.3	Homo sapiens enolase 1 (ENO1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu19186	NM_001428.4	Homo sapiens enolase 1 (ENO1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

ENO1 ( NM_001201483.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001188412.1 Homo sapiens enolase 1 (ENO1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17630
Clone ID Related Accession (Same CDS sequence)	NM_001201483.1 , NM_001201483.3 , NM_001201483.4
Accession Version	NM_001201483.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-05
Organism	Homo sapiens(Human)
Product	c-myc promoter-binding protein-1 isoform MBP-1
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from GU170215.1 and BC021166.2. Transcript Variant: This variant (2) differs at the 5' end compared to variant 1, and initiates translation from an in-frame downstream start codon, resulting in a shorter isoform (MBP-1). This isoform is localized to the nucleus, and functions as a transcriptional repressor of c-myc protooncogene by binding to its promoter (PMID:20849415). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GU170215.1, SRR1803613.39602.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCGAGA TGGATGGAAC AGAAAATAAA TCTAAGTTTG GTGCGAACGC CATTCTGGGG 
GTGTCCCTTG CCGTCTGCAA AGCTGGTGCC GTTGAGAAGG GGGTCCCCCT GTACCGCCAC 
ATCGCTGACT TGGCTGGCAA CTCTGAAGTC ATCCTGCCAG TCCCGGCGTT CAATGTCATC 
AATGGCGGTT CTCATGCTGG CAACAAGCTG GCCATGCAGG AGTTCATGAT CCTCCCAGTC 
GGTGCAGCAA ACTTCAGGGA AGCCATGCGC ATTGGAGCAG AGGTTTACCA CAACCTGAAG 
AATGTCATCA AGGAGAAATA TGGGAAAGAT GCCACCAATG TGGGGGATGA AGGCGGGTTT 
GCTCCCAACA TCCTGGAGAA TAAAGAAGGC CTGGAGCTGC TGAAGACTGC TATTGGGAAA 
GCTGGCTACA CTGATAAGGT GGTCATCGGC ATGGACGTAG CGGCCTCCGA GTTCTTCAGG 
TCTGGGAAGT ATGACCTGGA CTTCAAGTCT CCCGATGACC CCAGCAGGTA CATCTCGCCT 
GACCAGCTGG CTGACCTGTA CAAGTCCTTC ATCAAGGACT ACCCAGTGGT GTCTATCGAA 
GATCCCTTTG ACCAGGATGA CTGGGGAGCT TGGCAGAAGT TCACAGCCAG TGCAGGAATC 
CAGGTAGTGG GGGATGATCT CACAGTGACC AACCCAAAGA GGATCGCCAA GGCCGTGAAC 
GAGAAGTCCT GCAACTGCCT CCTGCTCAAA GTCAACCAGA TTGGCTCCGT GACCGAGTCT 
CTTCAGGCGT GCAAGCTGGC CCAGGCCAAT GGTTGGGGCG TCATGGTGTC TCATCGTTCG 
GGGGAGACTG AAGATACCTT CATCGCTGAC CTGGTTGTGG GGCTGTGCAC TGGGCAGATC 
AAGACTGGTG CCCCTTGCCG ATCTGAGCGC TTGGCCAAGT ACAACCAGCT CCTCAGAATT 
GAAGAGGAGC TGGGCAGCAA GGCTAAGTTT GCCGGCAGGA ACTTCAGAAA CCCCTTGGCC 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188412.1
CDS	1165..2190
Translation	MIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK

Target ORF information:

RefSeq Version	NM_001201483.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens enolase 1 (ENO1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201483.1

ORF Insert Sequence:

ATGATCGAGA TGGATGGAAC AGAAAATAAA TCTAAGTTTG GTGCGAACGC CATTCTGGGG 
GTGTCCCTTG CCGTCTGCAA AGCTGGTGCC GTTGAGAAGG GGGTCCCCCT GTACCGCCAC 
ATCGCTGACT TGGCTGGCAA CTCTGAAGTC ATCCTGCCAG TCCCGGCGTT CAATGTCATC 
AATGGCGGTT CTCATGCTGG CAACAAGCTG GCCATGCAGG AGTTCATGAT CCTCCCAGTC 
GGTGCAGCAA ACTTCAGGGA AGCCATGCGC ATTGGAGCAG AGGTTTACCA CAACCTGAAG 
AATGTCATCA AGGAGAAATA TGGGAAAGAT GCCACCAATG TGGGGGATGA AGGCGGGTTT 
GCTCCCAACA TCCTGGAGAA TAAAGAAGGC CTGGAGCTGC TGAAGACTGC TATTGGGAAA 
GCTGGCTACA CTGATAAGGT GGTCATCGGC ATGGACGTAG CGGCCTCCGA GTTCTTCAGG 
TCTGGGAAGT ATGACCTGGA CTTCAAGTCT CCCGATGACC CCAGCAGGTA CATCTCGCCT 
GACCAGCTGG CTGACCTGTA CAAGTCCTTC ATCAAGGACT ACCCAGTGGT GTCTATCGAA 
GATCCCTTTG ACCAGGATGA CTGGGGAGCT TGGCAGAAGT TCACAGCCAG TGCAGGAATC 
CAGGTAGTGG GGGATGATCT CACAGTGACC AACCCAAAGA GGATCGCCAA GGCCGTGAAC 
GAGAAGTCCT GCAACTGCCT CCTGCTCAAA GTCAACCAGA TTGGCTCCGT GACCGAGTCT 
CTTCAGGCGT GCAAGCTGGC CCAGGCCAAT GGTTGGGGCG TCATGGTGTC TCATCGTTCG 
GGGGAGACTG AAGATACCTT CATCGCTGAC CTGGTTGTGG GGCTGTGCAC TGGGCAGATC 
AAGACTGGTG CCCCTTGCCG ATCTGAGCGC TTGGCCAAGT ACAACCAGCT CCTCAGAATT 
GAAGAGGAGC TGGGCAGCAA GGCTAAGTTT GCCGGCAGGA ACTTCAGAAA CCCCTTGGCC 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENO1 ( NM_001201483.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001188412.1 Homo sapiens enolase 1 (ENO1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17630
Clone ID Related Accession (Same CDS sequence)	NM_001201483.1 , NM_001201483.3 , NM_001201483.4
Accession Version	NM_001201483.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Homo sapiens(human)
Product	c-myc promoter-binding protein-1 isoform MBP-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139415.10, GU170215.1 and BC001810.2. On Dec 20, 2017 this sequence version replaced NM_001201483.2. Transcript Variant: This variant (2) differs at the 5' end compared to variant 1, and initiates translation from a downstream in-frame start codon, resulting in a shorter isoform (MBP-1). This isoform is localized to the nucleus, and functions as a transcriptional repressor of c-myc protooncogene by binding to its promoter (PMID:20849415). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.241947.1, SRR1803615.200695.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCGAGA TGGATGGAAC AGAAAATAAA TCTAAGTTTG GTGCGAACGC CATTCTGGGG 
GTGTCCCTTG CCGTCTGCAA AGCTGGTGCC GTTGAGAAGG GGGTCCCCCT GTACCGCCAC 
ATCGCTGACT TGGCTGGCAA CTCTGAAGTC ATCCTGCCAG TCCCGGCGTT CAATGTCATC 
AATGGCGGTT CTCATGCTGG CAACAAGCTG GCCATGCAGG AGTTCATGAT CCTCCCAGTC 
GGTGCAGCAA ACTTCAGGGA AGCCATGCGC ATTGGAGCAG AGGTTTACCA CAACCTGAAG 
AATGTCATCA AGGAGAAATA TGGGAAAGAT GCCACCAATG TGGGGGATGA AGGCGGGTTT 
GCTCCCAACA TCCTGGAGAA TAAAGAAGGC CTGGAGCTGC TGAAGACTGC TATTGGGAAA 
GCTGGCTACA CTGATAAGGT GGTCATCGGC ATGGACGTAG CGGCCTCCGA GTTCTTCAGG 
TCTGGGAAGT ATGACCTGGA CTTCAAGTCT CCCGATGACC CCAGCAGGTA CATCTCGCCT 
GACCAGCTGG CTGACCTGTA CAAGTCCTTC ATCAAGGACT ACCCAGTGGT GTCTATCGAA 
GATCCCTTTG ACCAGGATGA CTGGGGAGCT TGGCAGAAGT TCACAGCCAG TGCAGGAATC 
CAGGTAGTGG GGGATGATCT CACAGTGACC AACCCAAAGA GGATCGCCAA GGCCGTGAAC 
GAGAAGTCCT GCAACTGCCT CCTGCTCAAA GTCAACCAGA TTGGCTCCGT GACCGAGTCT 
CTTCAGGCGT GCAAGCTGGC CCAGGCCAAT GGTTGGGGCG TCATGGTGTC TCATCGTTCG 
GGGGAGACTG AAGATACCTT CATCGCTGAC CTGGTTGTGG GGCTGTGCAC TGGGCAGATC 
AAGACTGGTG CCCCTTGCCG ATCTGAGCGC TTGGCCAAGT ACAACCAGCT CCTCAGAATT 
GAAGAGGAGC TGGGCAGCAA GGCTAAGTTT GCCGGCAGGA ACTTCAGAAA CCCCTTGGCC 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188412.1
CDS	1776..2801
Translation	MIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK

Target ORF information:

RefSeq Version	NM_001201483.3
Organism	Homo sapiens(human)
Definition	Homo sapiens enolase 1 (ENO1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201483.3

ORF Insert Sequence:

ATGATCGAGA TGGATGGAAC AGAAAATAAA TCTAAGTTTG GTGCGAACGC CATTCTGGGG 
GTGTCCCTTG CCGTCTGCAA AGCTGGTGCC GTTGAGAAGG GGGTCCCCCT GTACCGCCAC 
ATCGCTGACT TGGCTGGCAA CTCTGAAGTC ATCCTGCCAG TCCCGGCGTT CAATGTCATC 
AATGGCGGTT CTCATGCTGG CAACAAGCTG GCCATGCAGG AGTTCATGAT CCTCCCAGTC 
GGTGCAGCAA ACTTCAGGGA AGCCATGCGC ATTGGAGCAG AGGTTTACCA CAACCTGAAG 
AATGTCATCA AGGAGAAATA TGGGAAAGAT GCCACCAATG TGGGGGATGA AGGCGGGTTT 
GCTCCCAACA TCCTGGAGAA TAAAGAAGGC CTGGAGCTGC TGAAGACTGC TATTGGGAAA 
GCTGGCTACA CTGATAAGGT GGTCATCGGC ATGGACGTAG CGGCCTCCGA GTTCTTCAGG 
TCTGGGAAGT ATGACCTGGA CTTCAAGTCT CCCGATGACC CCAGCAGGTA CATCTCGCCT 
GACCAGCTGG CTGACCTGTA CAAGTCCTTC ATCAAGGACT ACCCAGTGGT GTCTATCGAA 
GATCCCTTTG ACCAGGATGA CTGGGGAGCT TGGCAGAAGT TCACAGCCAG TGCAGGAATC 
CAGGTAGTGG GGGATGATCT CACAGTGACC AACCCAAAGA GGATCGCCAA GGCCGTGAAC 
GAGAAGTCCT GCAACTGCCT CCTGCTCAAA GTCAACCAGA TTGGCTCCGT GACCGAGTCT 
CTTCAGGCGT GCAAGCTGGC CCAGGCCAAT GGTTGGGGCG TCATGGTGTC TCATCGTTCG 
GGGGAGACTG AAGATACCTT CATCGCTGAC CTGGTTGTGG GGCTGTGCAC TGGGCAGATC 
AAGACTGGTG CCCCTTGCCG ATCTGAGCGC TTGGCCAAGT ACAACCAGCT CCTCAGAATT 
GAAGAGGAGC TGGGCAGCAA GGCTAAGTTT GCCGGCAGGA ACTTCAGAAA CCCCTTGGCC 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENO1 ( NM_001201483.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001188412.1 Homo sapiens enolase 1 (ENO1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17630
Clone ID Related Accession (Same CDS sequence)	NM_001201483.1 , NM_001201483.3 , NM_001201483.4
Accession Version	NM_001201483.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	c-myc promoter-binding protein-1 isoform MBP-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139415.10, GU170215.1 and BC001810.2. On May 31, 2019 this sequence version replaced NM_001201483.3. Transcript Variant: This variant (2) differs at the 5' end compared to variant 1, and initiates translation from a downstream in-frame start codon, resulting in a shorter isoform (MBP-1). This isoform is localized to the nucleus, and functions as a transcriptional repressor of c-myc protooncogene by binding to its promoter (PMID:20849415). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.241947.1, SRR1803613.75258.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCGAGA TGGATGGAAC AGAAAATAAA TCTAAGTTTG GTGCGAACGC CATTCTGGGG 
GTGTCCCTTG CCGTCTGCAA AGCTGGTGCC GTTGAGAAGG GGGTCCCCCT GTACCGCCAC 
ATCGCTGACT TGGCTGGCAA CTCTGAAGTC ATCCTGCCAG TCCCGGCGTT CAATGTCATC 
AATGGCGGTT CTCATGCTGG CAACAAGCTG GCCATGCAGG AGTTCATGAT CCTCCCAGTC 
GGTGCAGCAA ACTTCAGGGA AGCCATGCGC ATTGGAGCAG AGGTTTACCA CAACCTGAAG 
AATGTCATCA AGGAGAAATA TGGGAAAGAT GCCACCAATG TGGGGGATGA AGGCGGGTTT 
GCTCCCAACA TCCTGGAGAA TAAAGAAGGC CTGGAGCTGC TGAAGACTGC TATTGGGAAA 
GCTGGCTACA CTGATAAGGT GGTCATCGGC ATGGACGTAG CGGCCTCCGA GTTCTTCAGG 
TCTGGGAAGT ATGACCTGGA CTTCAAGTCT CCCGATGACC CCAGCAGGTA CATCTCGCCT 
GACCAGCTGG CTGACCTGTA CAAGTCCTTC ATCAAGGACT ACCCAGTGGT GTCTATCGAA 
GATCCCTTTG ACCAGGATGA CTGGGGAGCT TGGCAGAAGT TCACAGCCAG TGCAGGAATC 
CAGGTAGTGG GGGATGATCT CACAGTGACC AACCCAAAGA GGATCGCCAA GGCCGTGAAC 
GAGAAGTCCT GCAACTGCCT CCTGCTCAAA GTCAACCAGA TTGGCTCCGT GACCGAGTCT 
CTTCAGGCGT GCAAGCTGGC CCAGGCCAAT GGTTGGGGCG TCATGGTGTC TCATCGTTCG 
GGGGAGACTG AAGATACCTT CATCGCTGAC CTGGTTGTGG GGCTGTGCAC TGGGCAGATC 
AAGACTGGTG CCCCTTGCCG ATCTGAGCGC TTGGCCAAGT ACAACCAGCT CCTCAGAATT 
GAAGAGGAGC TGGGCAGCAA GGCTAAGTTT GCCGGCAGGA ACTTCAGAAA CCCCTTGGCC 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188412.1
CDS	1776..2801
Translation	MIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK

Target ORF information:

RefSeq Version	NM_001201483.4
Organism	Homo sapiens(human)
Definition	Homo sapiens enolase 1 (ENO1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201483.4

ORF Insert Sequence:

ATGATCGAGA TGGATGGAAC AGAAAATAAA TCTAAGTTTG GTGCGAACGC CATTCTGGGG 
GTGTCCCTTG CCGTCTGCAA AGCTGGTGCC GTTGAGAAGG GGGTCCCCCT GTACCGCCAC 
ATCGCTGACT TGGCTGGCAA CTCTGAAGTC ATCCTGCCAG TCCCGGCGTT CAATGTCATC 
AATGGCGGTT CTCATGCTGG CAACAAGCTG GCCATGCAGG AGTTCATGAT CCTCCCAGTC 
GGTGCAGCAA ACTTCAGGGA AGCCATGCGC ATTGGAGCAG AGGTTTACCA CAACCTGAAG 
AATGTCATCA AGGAGAAATA TGGGAAAGAT GCCACCAATG TGGGGGATGA AGGCGGGTTT 
GCTCCCAACA TCCTGGAGAA TAAAGAAGGC CTGGAGCTGC TGAAGACTGC TATTGGGAAA 
GCTGGCTACA CTGATAAGGT GGTCATCGGC ATGGACGTAG CGGCCTCCGA GTTCTTCAGG 
TCTGGGAAGT ATGACCTGGA CTTCAAGTCT CCCGATGACC CCAGCAGGTA CATCTCGCCT 
GACCAGCTGG CTGACCTGTA CAAGTCCTTC ATCAAGGACT ACCCAGTGGT GTCTATCGAA 
GATCCCTTTG ACCAGGATGA CTGGGGAGCT TGGCAGAAGT TCACAGCCAG TGCAGGAATC 
CAGGTAGTGG GGGATGATCT CACAGTGACC AACCCAAAGA GGATCGCCAA GGCCGTGAAC 
GAGAAGTCCT GCAACTGCCT CCTGCTCAAA GTCAACCAGA TTGGCTCCGT GACCGAGTCT 
CTTCAGGCGT GCAAGCTGGC CCAGGCCAAT GGTTGGGGCG TCATGGTGTC TCATCGTTCG 
GGGGAGACTG AAGATACCTT CATCGCTGAC CTGGTTGTGG GGCTGTGCAC TGGGCAGATC 
AAGACTGGTG CCCCTTGCCG ATCTGAGCGC TTGGCCAAGT ACAACCAGCT CCTCAGAATT 
GAAGAGGAGC TGGGCAGCAA GGCTAAGTTT GCCGGCAGGA ACTTCAGAAA CCCCTTGGCC 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENO1 ( NM_001428.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001419.1 Homo sapiens enolase 1 (ENO1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19186
Clone ID Related Accession (Same CDS sequence)	NM_001428.4 , NM_001428.3 , NM_001428.5
Accession Version	NM_001428.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	alpha-enolase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK309177.1, BC021166.2 and BC001810.2. On Dec 20, 2017 this sequence version replaced NM_001428.3. Transcript Variant: This variant (1) encodes isoform (1), which is localized to the cytosol, and has alpha-enolase activity. It has been reported that the monomeric form of this isoform functions as a structural lens protein (tau-crystallin), and the dimeric form as an enolase (PMID:2462567). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022545.1, BX537400.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTATTC TCAAGATCCA TGCCAGGGAG ATCTTTGACT CTCGCGGGAA TCCCACTGTT 
GAGGTTGATC TCTTCACCTC AAAAGGTCTC TTCAGAGCTG CTGTGCCCAG TGGTGCTTCA 
ACTGGTATCT ATGAGGCCCT AGAGCTCCGG GACAATGATA AGACTCGCTA TATGGGGAAG 
GGTGTCTCAA AGGCTGTTGA GCACATCAAT AAAACTATTG CGCCTGCCCT GGTTAGCAAG 
AAACTGAACG TCACAGAACA AGAGAAGATT GACAAACTGA TGATCGAGAT GGATGGAACA 
GAAAATAAAT CTAAGTTTGG TGCGAACGCC ATTCTGGGGG TGTCCCTTGC CGTCTGCAAA 
GCTGGTGCCG TTGAGAAGGG GGTCCCCCTG TACCGCCACA TCGCTGACTT GGCTGGCAAC 
TCTGAAGTCA TCCTGCCAGT CCCGGCGTTC AATGTCATCA ATGGCGGTTC TCATGCTGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTCCCAGTCG GTGCAGCAAA CTTCAGGGAA 
GCCATGCGCA TTGGAGCAGA GGTTTACCAC AACCTGAAGA ATGTCATCAA GGAGAAATAT 
GGGAAAGATG CCACCAATGT GGGGGATGAA GGCGGGTTTG CTCCCAACAT CCTGGAGAAT 
AAAGAAGGCC TGGAGCTGCT GAAGACTGCT ATTGGGAAAG CTGGCTACAC TGATAAGGTG 
GTCATCGGCA TGGACGTAGC GGCCTCCGAG TTCTTCAGGT CTGGGAAGTA TGACCTGGAC 
TTCAAGTCTC CCGATGACCC CAGCAGGTAC ATCTCGCCTG ACCAGCTGGC TGACCTGTAC 
AAGTCCTTCA TCAAGGACTA CCCAGTGGTG TCTATCGAAG ATCCCTTTGA CCAGGATGAC 
TGGGGAGCTT GGCAGAAGTT CACAGCCAGT GCAGGAATCC AGGTAGTGGG GGATGATCTC 
ACAGTGACCA ACCCAAAGAG GATCGCCAAG GCCGTGAACG AGAAGTCCTG CAACTGCCTC 
CTGCTCAAAG TCAACCAGAT TGGCTCCGTG ACCGAGTCTC TTCAGGCGTG CAAGCTGGCC 
CAGGCCAATG GTTGGGGCGT CATGGTGTCT CATCGTTCGG GGGAGACTGA AGATACCTTC 
ATCGCTGACC TGGTTGTGGG GCTGTGCACT GGGCAGATCA AGACTGGTGC CCCTTGCCGA 
TCTGAGCGCT TGGCCAAGTA CAACCAGCTC CTCAGAATTG AAGAGGAGCT GGGCAGCAAG 
GCTAAGTTTG CCGGCAGGAA CTTCAGAAAC CCCTTGGCCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001419.1
CDS	117..1421
Translation	MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK

Target ORF information:

RefSeq Version	NM_001428.4
Organism	Homo sapiens(human)
Definition	Homo sapiens enolase 1 (ENO1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001428.4

ORF Insert Sequence:

ATGTCTATTC TCAAGATCCA TGCCAGGGAG ATCTTTGACT CTCGCGGGAA TCCCACTGTT 
GAGGTTGATC TCTTCACCTC AAAAGGTCTC TTCAGAGCTG CTGTGCCCAG TGGTGCTTCA 
ACTGGTATCT ATGAGGCCCT AGAGCTCCGG GACAATGATA AGACTCGCTA TATGGGGAAG 
GGTGTCTCAA AGGCTGTTGA GCACATCAAT AAAACTATTG CGCCTGCCCT GGTTAGCAAG 
AAACTGAACG TCACAGAACA AGAGAAGATT GACAAACTGA TGATCGAGAT GGATGGAACA 
GAAAATAAAT CTAAGTTTGG TGCGAACGCC ATTCTGGGGG TGTCCCTTGC CGTCTGCAAA 
GCTGGTGCCG TTGAGAAGGG GGTCCCCCTG TACCGCCACA TCGCTGACTT GGCTGGCAAC 
TCTGAAGTCA TCCTGCCAGT CCCGGCGTTC AATGTCATCA ATGGCGGTTC TCATGCTGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTCCCAGTCG GTGCAGCAAA CTTCAGGGAA 
GCCATGCGCA TTGGAGCAGA GGTTTACCAC AACCTGAAGA ATGTCATCAA GGAGAAATAT 
GGGAAAGATG CCACCAATGT GGGGGATGAA GGCGGGTTTG CTCCCAACAT CCTGGAGAAT 
AAAGAAGGCC TGGAGCTGCT GAAGACTGCT ATTGGGAAAG CTGGCTACAC TGATAAGGTG 
GTCATCGGCA TGGACGTAGC GGCCTCCGAG TTCTTCAGGT CTGGGAAGTA TGACCTGGAC 
TTCAAGTCTC CCGATGACCC CAGCAGGTAC ATCTCGCCTG ACCAGCTGGC TGACCTGTAC 
AAGTCCTTCA TCAAGGACTA CCCAGTGGTG TCTATCGAAG ATCCCTTTGA CCAGGATGAC 
TGGGGAGCTT GGCAGAAGTT CACAGCCAGT GCAGGAATCC AGGTAGTGGG GGATGATCTC 
ACAGTGACCA ACCCAAAGAG GATCGCCAAG GCCGTGAACG AGAAGTCCTG CAACTGCCTC 
CTGCTCAAAG TCAACCAGAT TGGCTCCGTG ACCGAGTCTC TTCAGGCGTG CAAGCTGGCC 
CAGGCCAATG GTTGGGGCGT CATGGTGTCT CATCGTTCGG GGGAGACTGA AGATACCTTC 
ATCGCTGACC TGGTTGTGGG GCTGTGCACT GGGCAGATCA AGACTGGTGC CCCTTGCCGA 
TCTGAGCGCT TGGCCAAGTA CAACCAGCTC CTCAGAATTG AAGAGGAGCT GGGCAGCAAG 
GCTAAGTTTG CCGGCAGGAA CTTCAGAAAC CCCTTGGCCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENO1 ( NM_001428.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001419.1 Homo sapiens enolase 1 (ENO1), transcript variant 1, mRNA.

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CloneID	OHu19186
Clone ID Related Accession (Same CDS sequence)	NM_001428.4 , NM_001428.3 , NM_001428.5
Accession Version	NM_001428.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-05
Organism	Homo sapiens(Human)
Product	alpha-enolase isoform 1
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK309177.1 and BC021166.2. This sequence is a reference standard in the RefSeqGene project. On Jan 25, 2011 this sequence version replaced NM_001428.2. Transcript Variant: This variant (1) encodes the longer isoform (1), which is localized to the cytosol, and has alpha-enolase activity. It has been reported that the monomeric form of this isoform functions as a structural lens protein (tau-crystallin), and the dimeric form as an enolase (PMID:2462567). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.104940.1, SRR1660807.136492.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142670, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTATTC TCAAGATCCA TGCCAGGGAG ATCTTTGACT CTCGCGGGAA TCCCACTGTT 
GAGGTTGATC TCTTCACCTC AAAAGGTCTC TTCAGAGCTG CTGTGCCCAG TGGTGCTTCA 
ACTGGTATCT ATGAGGCCCT AGAGCTCCGG GACAATGATA AGACTCGCTA TATGGGGAAG 
GGTGTCTCAA AGGCTGTTGA GCACATCAAT AAAACTATTG CGCCTGCCCT GGTTAGCAAG 
AAACTGAACG TCACAGAACA AGAGAAGATT GACAAACTGA TGATCGAGAT GGATGGAACA 
GAAAATAAAT CTAAGTTTGG TGCGAACGCC ATTCTGGGGG TGTCCCTTGC CGTCTGCAAA 
GCTGGTGCCG TTGAGAAGGG GGTCCCCCTG TACCGCCACA TCGCTGACTT GGCTGGCAAC 
TCTGAAGTCA TCCTGCCAGT CCCGGCGTTC AATGTCATCA ATGGCGGTTC TCATGCTGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTCCCAGTCG GTGCAGCAAA CTTCAGGGAA 
GCCATGCGCA TTGGAGCAGA GGTTTACCAC AACCTGAAGA ATGTCATCAA GGAGAAATAT 
GGGAAAGATG CCACCAATGT GGGGGATGAA GGCGGGTTTG CTCCCAACAT CCTGGAGAAT 
AAAGAAGGCC TGGAGCTGCT GAAGACTGCT ATTGGGAAAG CTGGCTACAC TGATAAGGTG 
GTCATCGGCA TGGACGTAGC GGCCTCCGAG TTCTTCAGGT CTGGGAAGTA TGACCTGGAC 
TTCAAGTCTC CCGATGACCC CAGCAGGTAC ATCTCGCCTG ACCAGCTGGC TGACCTGTAC 
AAGTCCTTCA TCAAGGACTA CCCAGTGGTG TCTATCGAAG ATCCCTTTGA CCAGGATGAC 
TGGGGAGCTT GGCAGAAGTT CACAGCCAGT GCAGGAATCC AGGTAGTGGG GGATGATCTC 
ACAGTGACCA ACCCAAAGAG GATCGCCAAG GCCGTGAACG AGAAGTCCTG CAACTGCCTC 
CTGCTCAAAG TCAACCAGAT TGGCTCCGTG ACCGAGTCTC TTCAGGCGTG CAAGCTGGCC 
CAGGCCAATG GTTGGGGCGT CATGGTGTCT CATCGTTCGG GGGAGACTGA AGATACCTTC 
ATCGCTGACC TGGTTGTGGG GCTGTGCACT GGGCAGATCA AGACTGGTGC CCCTTGCCGA 
TCTGAGCGCT TGGCCAAGTA CAACCAGCTC CTCAGAATTG AAGAGGAGCT GGGCAGCAAG 
GCTAAGTTTG CCGGCAGGAA CTTCAGAAAC CCCTTGGCCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001419.1
CDS	523..1827
Translation	MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK

Target ORF information:

RefSeq Version	NM_001428.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens enolase 1 (ENO1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001428.3

ORF Insert Sequence:

ATGTCTATTC TCAAGATCCA TGCCAGGGAG ATCTTTGACT CTCGCGGGAA TCCCACTGTT 
GAGGTTGATC TCTTCACCTC AAAAGGTCTC TTCAGAGCTG CTGTGCCCAG TGGTGCTTCA 
ACTGGTATCT ATGAGGCCCT AGAGCTCCGG GACAATGATA AGACTCGCTA TATGGGGAAG 
GGTGTCTCAA AGGCTGTTGA GCACATCAAT AAAACTATTG CGCCTGCCCT GGTTAGCAAG 
AAACTGAACG TCACAGAACA AGAGAAGATT GACAAACTGA TGATCGAGAT GGATGGAACA 
GAAAATAAAT CTAAGTTTGG TGCGAACGCC ATTCTGGGGG TGTCCCTTGC CGTCTGCAAA 
GCTGGTGCCG TTGAGAAGGG GGTCCCCCTG TACCGCCACA TCGCTGACTT GGCTGGCAAC 
TCTGAAGTCA TCCTGCCAGT CCCGGCGTTC AATGTCATCA ATGGCGGTTC TCATGCTGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTCCCAGTCG GTGCAGCAAA CTTCAGGGAA 
GCCATGCGCA TTGGAGCAGA GGTTTACCAC AACCTGAAGA ATGTCATCAA GGAGAAATAT 
GGGAAAGATG CCACCAATGT GGGGGATGAA GGCGGGTTTG CTCCCAACAT CCTGGAGAAT 
AAAGAAGGCC TGGAGCTGCT GAAGACTGCT ATTGGGAAAG CTGGCTACAC TGATAAGGTG 
GTCATCGGCA TGGACGTAGC GGCCTCCGAG TTCTTCAGGT CTGGGAAGTA TGACCTGGAC 
TTCAAGTCTC CCGATGACCC CAGCAGGTAC ATCTCGCCTG ACCAGCTGGC TGACCTGTAC 
AAGTCCTTCA TCAAGGACTA CCCAGTGGTG TCTATCGAAG ATCCCTTTGA CCAGGATGAC 
TGGGGAGCTT GGCAGAAGTT CACAGCCAGT GCAGGAATCC AGGTAGTGGG GGATGATCTC 
ACAGTGACCA ACCCAAAGAG GATCGCCAAG GCCGTGAACG AGAAGTCCTG CAACTGCCTC 
CTGCTCAAAG TCAACCAGAT TGGCTCCGTG ACCGAGTCTC TTCAGGCGTG CAAGCTGGCC 
CAGGCCAATG GTTGGGGCGT CATGGTGTCT CATCGTTCGG GGGAGACTGA AGATACCTTC 
ATCGCTGACC TGGTTGTGGG GCTGTGCACT GGGCAGATCA AGACTGGTGC CCCTTGCCGA 
TCTGAGCGCT TGGCCAAGTA CAACCAGCTC CTCAGAATTG AAGAGGAGCT GGGCAGCAAG 
GCTAAGTTTG CCGGCAGGAA CTTCAGAAAC CCCTTGGCCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENO1 ( NM_001428.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001419.1 Homo sapiens enolase 1 (ENO1), transcript variant 1, mRNA.

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CloneID	OHu19186
Clone ID Related Accession (Same CDS sequence)	NM_001428.4 , NM_001428.3 , NM_001428.5
Accession Version	NM_001428.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	alpha-enolase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK309177.1 and BC021166.2. This sequence is a reference standard in the RefSeqGene project. On May 10, 2018 this sequence version replaced NM_001428.4. Transcript Variant: This variant (1) encodes isoform (1), which is localized to the cytosol, and has alpha-enolase activity. It has been reported that the monomeric form of this isoform functions as a structural lens protein (tau-crystallin), and the dimeric form as an enolase (PMID:2462567). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.75182.1, SRR5189658.211206.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000234590.10/ ENSP00000234590.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTATTC TCAAGATCCA TGCCAGGGAG ATCTTTGACT CTCGCGGGAA TCCCACTGTT 
GAGGTTGATC TCTTCACCTC AAAAGGTCTC TTCAGAGCTG CTGTGCCCAG TGGTGCTTCA 
ACTGGTATCT ATGAGGCCCT AGAGCTCCGG GACAATGATA AGACTCGCTA TATGGGGAAG 
GGTGTCTCAA AGGCTGTTGA GCACATCAAT AAAACTATTG CGCCTGCCCT GGTTAGCAAG 
AAACTGAACG TCACAGAACA AGAGAAGATT GACAAACTGA TGATCGAGAT GGATGGAACA 
GAAAATAAAT CTAAGTTTGG TGCGAACGCC ATTCTGGGGG TGTCCCTTGC CGTCTGCAAA 
GCTGGTGCCG TTGAGAAGGG GGTCCCCCTG TACCGCCACA TCGCTGACTT GGCTGGCAAC 
TCTGAAGTCA TCCTGCCAGT CCCGGCGTTC AATGTCATCA ATGGCGGTTC TCATGCTGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTCCCAGTCG GTGCAGCAAA CTTCAGGGAA 
GCCATGCGCA TTGGAGCAGA GGTTTACCAC AACCTGAAGA ATGTCATCAA GGAGAAATAT 
GGGAAAGATG CCACCAATGT GGGGGATGAA GGCGGGTTTG CTCCCAACAT CCTGGAGAAT 
AAAGAAGGCC TGGAGCTGCT GAAGACTGCT ATTGGGAAAG CTGGCTACAC TGATAAGGTG 
GTCATCGGCA TGGACGTAGC GGCCTCCGAG TTCTTCAGGT CTGGGAAGTA TGACCTGGAC 
TTCAAGTCTC CCGATGACCC CAGCAGGTAC ATCTCGCCTG ACCAGCTGGC TGACCTGTAC 
AAGTCCTTCA TCAAGGACTA CCCAGTGGTG TCTATCGAAG ATCCCTTTGA CCAGGATGAC 
TGGGGAGCTT GGCAGAAGTT CACAGCCAGT GCAGGAATCC AGGTAGTGGG GGATGATCTC 
ACAGTGACCA ACCCAAAGAG GATCGCCAAG GCCGTGAACG AGAAGTCCTG CAACTGCCTC 
CTGCTCAAAG TCAACCAGAT TGGCTCCGTG ACCGAGTCTC TTCAGGCGTG CAAGCTGGCC 
CAGGCCAATG GTTGGGGCGT CATGGTGTCT CATCGTTCGG GGGAGACTGA AGATACCTTC 
ATCGCTGACC TGGTTGTGGG GCTGTGCACT GGGCAGATCA AGACTGGTGC CCCTTGCCGA 
TCTGAGCGCT TGGCCAAGTA CAACCAGCTC CTCAGAATTG AAGAGGAGCT GGGCAGCAAG 
GCTAAGTTTG CCGGCAGGAA CTTCAGAAAC CCCTTGGCCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001419.1
CDS	117..1421
Translation	MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK

Target ORF information:

RefSeq Version	NM_001428.5
Organism	Homo sapiens(human)
Definition	Homo sapiens enolase 1 (ENO1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001428.5

ORF Insert Sequence:

ATGTCTATTC TCAAGATCCA TGCCAGGGAG ATCTTTGACT CTCGCGGGAA TCCCACTGTT 
GAGGTTGATC TCTTCACCTC AAAAGGTCTC TTCAGAGCTG CTGTGCCCAG TGGTGCTTCA 
ACTGGTATCT ATGAGGCCCT AGAGCTCCGG GACAATGATA AGACTCGCTA TATGGGGAAG 
GGTGTCTCAA AGGCTGTTGA GCACATCAAT AAAACTATTG CGCCTGCCCT GGTTAGCAAG 
AAACTGAACG TCACAGAACA AGAGAAGATT GACAAACTGA TGATCGAGAT GGATGGAACA 
GAAAATAAAT CTAAGTTTGG TGCGAACGCC ATTCTGGGGG TGTCCCTTGC CGTCTGCAAA 
GCTGGTGCCG TTGAGAAGGG GGTCCCCCTG TACCGCCACA TCGCTGACTT GGCTGGCAAC 
TCTGAAGTCA TCCTGCCAGT CCCGGCGTTC AATGTCATCA ATGGCGGTTC TCATGCTGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTCCCAGTCG GTGCAGCAAA CTTCAGGGAA 
GCCATGCGCA TTGGAGCAGA GGTTTACCAC AACCTGAAGA ATGTCATCAA GGAGAAATAT 
GGGAAAGATG CCACCAATGT GGGGGATGAA GGCGGGTTTG CTCCCAACAT CCTGGAGAAT 
AAAGAAGGCC TGGAGCTGCT GAAGACTGCT ATTGGGAAAG CTGGCTACAC TGATAAGGTG 
GTCATCGGCA TGGACGTAGC GGCCTCCGAG TTCTTCAGGT CTGGGAAGTA TGACCTGGAC 
TTCAAGTCTC CCGATGACCC CAGCAGGTAC ATCTCGCCTG ACCAGCTGGC TGACCTGTAC 
AAGTCCTTCA TCAAGGACTA CCCAGTGGTG TCTATCGAAG ATCCCTTTGA CCAGGATGAC 
TGGGGAGCTT GGCAGAAGTT CACAGCCAGT GCAGGAATCC AGGTAGTGGG GGATGATCTC 
ACAGTGACCA ACCCAAAGAG GATCGCCAAG GCCGTGAACG AGAAGTCCTG CAACTGCCTC 
CTGCTCAAAG TCAACCAGAT TGGCTCCGTG ACCGAGTCTC TTCAGGCGTG CAAGCTGGCC 
CAGGCCAATG GTTGGGGCGT CATGGTGTCT CATCGTTCGG GGGAGACTGA AGATACCTTC 
ATCGCTGACC TGGTTGTGGG GCTGTGCACT GGGCAGATCA AGACTGGTGC CCCTTGCCGA 
TCTGAGCGCT TGGCCAAGTA CAACCAGCTC CTCAGAATTG AAGAGGAGCT GGGCAGCAAG 
GCTAAGTTTG CCGGCAGGAA CTTCAGAAAC CCCTTGGCCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENO1 ( NM_001353346.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340275.1 Homo sapiens enolase 1 (ENO1), transcript variant 3, mRNA.

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CloneID	OHu33139
Clone ID Related Accession (Same CDS sequence)	NM_001353346.2 , NM_001353346.3 , XM_006710433.2
Accession Version	NM_001353346.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Homo sapiens(human)
Product	alpha-enolase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA820724.1, GU170215.1 and BC001810.2. On Dec 20, 2017 this sequence version replaced NM_001353346.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.142779.1, SRR5189655.89985.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTATTC TCAAGATCCA TGCCAGGGAG ATCTTTGACT CTCGCGGGAA TCCCACTGTT 
GAGGTTGATC TCTTCACCTC AAAAGGTCTC TTCAGAGCTG CTGTGCCCAG TGGTGCTTCA 
ACTGGTATCT ATGAGGCCCT AGAGCTCCGG GACAATGATA AGACTCGCTA TATGGGGAAG 
GGCGTCTCCA GACCCATTAA GTATATTAAT GAGTTCCTGG CACCAGCCCT GTGCACTCAG 
AAACTGAACG TCACAGAACA AGAGAAGATT GACAAACTGA TGATCGAGAT GGATGGAACA 
GAAAATAAAT CTAAGTTTGG TGCGAACGCC ATTCTGGGGG TGTCCCTTGC CGTCTGCAAA 
GCTGGTGCCG TTGAGAAGGG GGTCCCCCTG TACCGCCACA TCGCTGACTT GGCTGGCAAC 
TCTGAAGTCA TCCTGCCAGT CCCGGCGTTC AATGTCATCA ATGGCGGTTC TCATGCTGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTCCCAGTCG GTGCAGCAAA CTTCAGGGAA 
GCCATGCGCA TTGGAGCAGA GGTTTACCAC AACCTGAAGA ATGTCATCAA GGAGAAATAT 
GGGAAAGATG CCACCAATGT GGGGGATGAA GGCGGGTTTG CTCCCAACAT CCTGGAGAAT 
AAAGAAGGCC TGGAGCTGCT GAAGACTGCT ATTGGGAAAG CTGGCTACAC TGATAAGGTG 
GTCATCGGCA TGGACGTAGC GGCCTCCGAG TTCTTCAGGT CTGGGAAGTA TGACCTGGAC 
TTCAAGTCTC CCGATGACCC CAGCAGGTAC ATCTCGCCTG ACCAGCTGGC TGACCTGTAC 
AAGTCCTTCA TCAAGGACTA CCCAGTGGTG TCTATCGAAG ATCCCTTTGA CCAGGATGAC 
TGGGGAGCTT GGCAGAAGTT CACAGCCAGT GCAGGAATCC AGGTAGTGGG GGATGATCTC 
ACAGTGACCA ACCCAAAGAG GATCGCCAAG GCCGTGAACG AGAAGTCCTG CAACTGCCTC 
CTGCTCAAAG TCAACCAGAT TGGCTCCGTG ACCGAGTCTC TTCAGGCGTG CAAGCTGGCC 
CAGGCCAATG GTTGGGGCGT CATGGTGTCT CATCGTTCGG GGGAGACTGA AGATACCTTC 
ATCGCTGACC TGGTTGTGGG GCTGTGCACT GGGCAGATCA AGACTGGTGC CCCTTGCCGA 
TCTGAGCGCT TGGCCAAGTA CAACCAGCTC CTCAGAATTG AAGAGGAGCT GGGCAGCAAG 
GCTAAGTTTG CCGGCAGGAA CTTCAGAAAC CCCTTGGCCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340275.1
CDS	117..1421
Translation	MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSRPIKYINEFLAPALCTQKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK

Target ORF information:

RefSeq Version	NM_001353346.2
Organism	Homo sapiens(human)
Definition	Homo sapiens enolase 1 (ENO1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353346.2

ORF Insert Sequence:

ATGTCTATTC TCAAGATCCA TGCCAGGGAG ATCTTTGACT CTCGCGGGAA TCCCACTGTT 
GAGGTTGATC TCTTCACCTC AAAAGGTCTC TTCAGAGCTG CTGTGCCCAG TGGTGCTTCA 
ACTGGTATCT ATGAGGCCCT AGAGCTCCGG GACAATGATA AGACTCGCTA TATGGGGAAG 
GGCGTCTCCA GACCCATTAA GTATATTAAT GAGTTCCTGG CACCAGCCCT GTGCACTCAG 
AAACTGAACG TCACAGAACA AGAGAAGATT GACAAACTGA TGATCGAGAT GGATGGAACA 
GAAAATAAAT CTAAGTTTGG TGCGAACGCC ATTCTGGGGG TGTCCCTTGC CGTCTGCAAA 
GCTGGTGCCG TTGAGAAGGG GGTCCCCCTG TACCGCCACA TCGCTGACTT GGCTGGCAAC 
TCTGAAGTCA TCCTGCCAGT CCCGGCGTTC AATGTCATCA ATGGCGGTTC TCATGCTGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTCCCAGTCG GTGCAGCAAA CTTCAGGGAA 
GCCATGCGCA TTGGAGCAGA GGTTTACCAC AACCTGAAGA ATGTCATCAA GGAGAAATAT 
GGGAAAGATG CCACCAATGT GGGGGATGAA GGCGGGTTTG CTCCCAACAT CCTGGAGAAT 
AAAGAAGGCC TGGAGCTGCT GAAGACTGCT ATTGGGAAAG CTGGCTACAC TGATAAGGTG 
GTCATCGGCA TGGACGTAGC GGCCTCCGAG TTCTTCAGGT CTGGGAAGTA TGACCTGGAC 
TTCAAGTCTC CCGATGACCC CAGCAGGTAC ATCTCGCCTG ACCAGCTGGC TGACCTGTAC 
AAGTCCTTCA TCAAGGACTA CCCAGTGGTG TCTATCGAAG ATCCCTTTGA CCAGGATGAC 
TGGGGAGCTT GGCAGAAGTT CACAGCCAGT GCAGGAATCC AGGTAGTGGG GGATGATCTC 
ACAGTGACCA ACCCAAAGAG GATCGCCAAG GCCGTGAACG AGAAGTCCTG CAACTGCCTC 
CTGCTCAAAG TCAACCAGAT TGGCTCCGTG ACCGAGTCTC TTCAGGCGTG CAAGCTGGCC 
CAGGCCAATG GTTGGGGCGT CATGGTGTCT CATCGTTCGG GGGAGACTGA AGATACCTTC 
ATCGCTGACC TGGTTGTGGG GCTGTGCACT GGGCAGATCA AGACTGGTGC CCCTTGCCGA 
TCTGAGCGCT TGGCCAAGTA CAACCAGCTC CTCAGAATTG AAGAGGAGCT GGGCAGCAAG 
GCTAAGTTTG CCGGCAGGAA CTTCAGAAAC CCCTTGGCCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENO1 ( NM_001353346.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340275.1 Homo sapiens enolase 1 (ENO1), transcript variant 3, mRNA.

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CloneID	OHu33139
Clone ID Related Accession (Same CDS sequence)	NM_001353346.2 , NM_001353346.3 , XM_006710433.2
Accession Version	NM_001353346.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	alpha-enolase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA820724.1, GU170215.1, BC001810.2 and AL139415.10. On May 31, 2019 this sequence version replaced NM_001353346.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.142779.1, SRR5189655.89985.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTATTC TCAAGATCCA TGCCAGGGAG ATCTTTGACT CTCGCGGGAA TCCCACTGTT 
GAGGTTGATC TCTTCACCTC AAAAGGTCTC TTCAGAGCTG CTGTGCCCAG TGGTGCTTCA 
ACTGGTATCT ATGAGGCCCT AGAGCTCCGG GACAATGATA AGACTCGCTA TATGGGGAAG 
GGCGTCTCCA GACCCATTAA GTATATTAAT GAGTTCCTGG CACCAGCCCT GTGCACTCAG 
AAACTGAACG TCACAGAACA AGAGAAGATT GACAAACTGA TGATCGAGAT GGATGGAACA 
GAAAATAAAT CTAAGTTTGG TGCGAACGCC ATTCTGGGGG TGTCCCTTGC CGTCTGCAAA 
GCTGGTGCCG TTGAGAAGGG GGTCCCCCTG TACCGCCACA TCGCTGACTT GGCTGGCAAC 
TCTGAAGTCA TCCTGCCAGT CCCGGCGTTC AATGTCATCA ATGGCGGTTC TCATGCTGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTCCCAGTCG GTGCAGCAAA CTTCAGGGAA 
GCCATGCGCA TTGGAGCAGA GGTTTACCAC AACCTGAAGA ATGTCATCAA GGAGAAATAT 
GGGAAAGATG CCACCAATGT GGGGGATGAA GGCGGGTTTG CTCCCAACAT CCTGGAGAAT 
AAAGAAGGCC TGGAGCTGCT GAAGACTGCT ATTGGGAAAG CTGGCTACAC TGATAAGGTG 
GTCATCGGCA TGGACGTAGC GGCCTCCGAG TTCTTCAGGT CTGGGAAGTA TGACCTGGAC 
TTCAAGTCTC CCGATGACCC CAGCAGGTAC ATCTCGCCTG ACCAGCTGGC TGACCTGTAC 
AAGTCCTTCA TCAAGGACTA CCCAGTGGTG TCTATCGAAG ATCCCTTTGA CCAGGATGAC 
TGGGGAGCTT GGCAGAAGTT CACAGCCAGT GCAGGAATCC AGGTAGTGGG GGATGATCTC 
ACAGTGACCA ACCCAAAGAG GATCGCCAAG GCCGTGAACG AGAAGTCCTG CAACTGCCTC 
CTGCTCAAAG TCAACCAGAT TGGCTCCGTG ACCGAGTCTC TTCAGGCGTG CAAGCTGGCC 
CAGGCCAATG GTTGGGGCGT CATGGTGTCT CATCGTTCGG GGGAGACTGA AGATACCTTC 
ATCGCTGACC TGGTTGTGGG GCTGTGCACT GGGCAGATCA AGACTGGTGC CCCTTGCCGA 
TCTGAGCGCT TGGCCAAGTA CAACCAGCTC CTCAGAATTG AAGAGGAGCT GGGCAGCAAG 
GCTAAGTTTG CCGGCAGGAA CTTCAGAAAC CCCTTGGCCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340275.1
CDS	117..1421
Translation	MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSRPIKYINEFLAPALCTQKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK

Target ORF information:

RefSeq Version	NM_001353346.3
Organism	Homo sapiens(human)
Definition	Homo sapiens enolase 1 (ENO1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353346.3

ORF Insert Sequence:

ATGTCTATTC TCAAGATCCA TGCCAGGGAG ATCTTTGACT CTCGCGGGAA TCCCACTGTT 
GAGGTTGATC TCTTCACCTC AAAAGGTCTC TTCAGAGCTG CTGTGCCCAG TGGTGCTTCA 
ACTGGTATCT ATGAGGCCCT AGAGCTCCGG GACAATGATA AGACTCGCTA TATGGGGAAG 
GGCGTCTCCA GACCCATTAA GTATATTAAT GAGTTCCTGG CACCAGCCCT GTGCACTCAG 
AAACTGAACG TCACAGAACA AGAGAAGATT GACAAACTGA TGATCGAGAT GGATGGAACA 
GAAAATAAAT CTAAGTTTGG TGCGAACGCC ATTCTGGGGG TGTCCCTTGC CGTCTGCAAA 
GCTGGTGCCG TTGAGAAGGG GGTCCCCCTG TACCGCCACA TCGCTGACTT GGCTGGCAAC 
TCTGAAGTCA TCCTGCCAGT CCCGGCGTTC AATGTCATCA ATGGCGGTTC TCATGCTGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTCCCAGTCG GTGCAGCAAA CTTCAGGGAA 
GCCATGCGCA TTGGAGCAGA GGTTTACCAC AACCTGAAGA ATGTCATCAA GGAGAAATAT 
GGGAAAGATG CCACCAATGT GGGGGATGAA GGCGGGTTTG CTCCCAACAT CCTGGAGAAT 
AAAGAAGGCC TGGAGCTGCT GAAGACTGCT ATTGGGAAAG CTGGCTACAC TGATAAGGTG 
GTCATCGGCA TGGACGTAGC GGCCTCCGAG TTCTTCAGGT CTGGGAAGTA TGACCTGGAC 
TTCAAGTCTC CCGATGACCC CAGCAGGTAC ATCTCGCCTG ACCAGCTGGC TGACCTGTAC 
AAGTCCTTCA TCAAGGACTA CCCAGTGGTG TCTATCGAAG ATCCCTTTGA CCAGGATGAC 
TGGGGAGCTT GGCAGAAGTT CACAGCCAGT GCAGGAATCC AGGTAGTGGG GGATGATCTC 
ACAGTGACCA ACCCAAAGAG GATCGCCAAG GCCGTGAACG AGAAGTCCTG CAACTGCCTC 
CTGCTCAAAG TCAACCAGAT TGGCTCCGTG ACCGAGTCTC TTCAGGCGTG CAAGCTGGCC 
CAGGCCAATG GTTGGGGCGT CATGGTGTCT CATCGTTCGG GGGAGACTGA AGATACCTTC 
ATCGCTGACC TGGTTGTGGG GCTGTGCACT GGGCAGATCA AGACTGGTGC CCCTTGCCGA 
TCTGAGCGCT TGGCCAAGTA CAACCAGCTC CTCAGAATTG AAGAGGAGCT GGGCAGCAAG 
GCTAAGTTTG CCGGCAGGAA CTTCAGAAAC CCCTTGGCCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENO1 ( XM_006710433.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006710496.1 Homo sapiens enolase 1 (ENO1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu33139
Clone ID Related Accession (Same CDS sequence)	NM_001353346.2 , NM_001353346.3 , XM_006710433.2
Accession Version	XM_006710433.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	alpha-enolase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006710433.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTATTC TCAAGATCCA TGCCAGGGAG ATCTTTGACT CTCGCGGGAA TCCCACTGTT 
GAGGTTGATC TCTTCACCTC AAAAGGTCTC TTCAGAGCTG CTGTGCCCAG TGGTGCTTCA 
ACTGGTATCT ATGAGGCCCT AGAGCTCCGG GACAATGATA AGACTCGCTA TATGGGGAAG 
GGCGTCTCCA GACCCATTAA GTATATTAAT GAGTTCCTGG CACCAGCCCT GTGCACTCAG 
AAACTGAACG TCACAGAACA AGAGAAGATT GACAAACTGA TGATCGAGAT GGATGGAACA 
GAAAATAAAT CTAAGTTTGG TGCGAACGCC ATTCTGGGGG TGTCCCTTGC CGTCTGCAAA 
GCTGGTGCCG TTGAGAAGGG GGTCCCCCTG TACCGCCACA TCGCTGACTT GGCTGGCAAC 
TCTGAAGTCA TCCTGCCAGT CCCGGCGTTC AATGTCATCA ATGGCGGTTC TCATGCTGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTCCCAGTCG GTGCAGCAAA CTTCAGGGAA 
GCCATGCGCA TTGGAGCAGA GGTTTACCAC AACCTGAAGA ATGTCATCAA GGAGAAATAT 
GGGAAAGATG CCACCAATGT GGGGGATGAA GGCGGGTTTG CTCCCAACAT CCTGGAGAAT 
AAAGAAGGCC TGGAGCTGCT GAAGACTGCT ATTGGGAAAG CTGGCTACAC TGATAAGGTG 
GTCATCGGCA TGGACGTAGC GGCCTCCGAG TTCTTCAGGT CTGGGAAGTA TGACCTGGAC 
TTCAAGTCTC CCGATGACCC CAGCAGGTAC ATCTCGCCTG ACCAGCTGGC TGACCTGTAC 
AAGTCCTTCA TCAAGGACTA CCCAGTGGTG TCTATCGAAG ATCCCTTTGA CCAGGATGAC 
TGGGGAGCTT GGCAGAAGTT CACAGCCAGT GCAGGAATCC AGGTAGTGGG GGATGATCTC 
ACAGTGACCA ACCCAAAGAG GATCGCCAAG GCCGTGAACG AGAAGTCCTG CAACTGCCTC 
CTGCTCAAAG TCAACCAGAT TGGCTCCGTG ACCGAGTCTC TTCAGGCGTG CAAGCTGGCC 
CAGGCCAATG GTTGGGGCGT CATGGTGTCT CATCGTTCGG GGGAGACTGA AGATACCTTC 
ATCGCTGACC TGGTTGTGGG GCTGTGCACT GGGCAGATCA AGACTGGTGC CCCTTGCCGA 
TCTGAGCGCT TGGCCAAGTA CAACCAGCTC CTCAGAATTG AAGAGGAGCT GGGCAGCAAG 
GCTAAGTTTG CCGGCAGGAA CTTCAGAAAC CCCTTGGCCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006710496.1
CDS	120..1424
Translation	MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSRPIKYINEFLAPALCTQKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK

Target ORF information:

RefSeq Version	XM_006710433.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens enolase 1 (ENO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006710433.2

ORF Insert Sequence:

ATGTCTATTC TCAAGATCCA TGCCAGGGAG ATCTTTGACT CTCGCGGGAA TCCCACTGTT 
GAGGTTGATC TCTTCACCTC AAAAGGTCTC TTCAGAGCTG CTGTGCCCAG TGGTGCTTCA 
ACTGGTATCT ATGAGGCCCT AGAGCTCCGG GACAATGATA AGACTCGCTA TATGGGGAAG 
GGCGTCTCCA GACCCATTAA GTATATTAAT GAGTTCCTGG CACCAGCCCT GTGCACTCAG 
AAACTGAACG TCACAGAACA AGAGAAGATT GACAAACTGA TGATCGAGAT GGATGGAACA 
GAAAATAAAT CTAAGTTTGG TGCGAACGCC ATTCTGGGGG TGTCCCTTGC CGTCTGCAAA 
GCTGGTGCCG TTGAGAAGGG GGTCCCCCTG TACCGCCACA TCGCTGACTT GGCTGGCAAC 
TCTGAAGTCA TCCTGCCAGT CCCGGCGTTC AATGTCATCA ATGGCGGTTC TCATGCTGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTCCCAGTCG GTGCAGCAAA CTTCAGGGAA 
GCCATGCGCA TTGGAGCAGA GGTTTACCAC AACCTGAAGA ATGTCATCAA GGAGAAATAT 
GGGAAAGATG CCACCAATGT GGGGGATGAA GGCGGGTTTG CTCCCAACAT CCTGGAGAAT 
AAAGAAGGCC TGGAGCTGCT GAAGACTGCT ATTGGGAAAG CTGGCTACAC TGATAAGGTG 
GTCATCGGCA TGGACGTAGC GGCCTCCGAG TTCTTCAGGT CTGGGAAGTA TGACCTGGAC 
TTCAAGTCTC CCGATGACCC CAGCAGGTAC ATCTCGCCTG ACCAGCTGGC TGACCTGTAC 
AAGTCCTTCA TCAAGGACTA CCCAGTGGTG TCTATCGAAG ATCCCTTTGA CCAGGATGAC 
TGGGGAGCTT GGCAGAAGTT CACAGCCAGT GCAGGAATCC AGGTAGTGGG GGATGATCTC 
ACAGTGACCA ACCCAAAGAG GATCGCCAAG GCCGTGAACG AGAAGTCCTG CAACTGCCTC 
CTGCTCAAAG TCAACCAGAT TGGCTCCGTG ACCGAGTCTC TTCAGGCGTG CAAGCTGGCC 
CAGGCCAATG GTTGGGGCGT CATGGTGTCT CATCGTTCGG GGGAGACTGA AGATACCTTC 
ATCGCTGACC TGGTTGTGGG GCTGTGCACT GGGCAGATCA AGACTGGTGC CCCTTGCCGA 
TCTGAGCGCT TGGCCAAGTA CAACCAGCTC CTCAGAATTG AAGAGGAGCT GGGCAGCAAG 
GCTAAGTTTG CCGGCAGGAA CTTCAGAAAC CCCTTGGCCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ENO1
Entrez Gene ID	2023
Full Name	enolase 1
Synonyms	ENO1L1,HEL-S-17,MPB1,NNE,PPH
General protein information	Preferred Names enolase 1 Names alpha-enolase c-myc promoter-binding protein-1 2-phospho-D-glycerate hydro-lyase MYC promoter-binding protein 1 alpha enolase like 1 enolase 1, (alpha) enolase-alpha epididymis secretory protein Li 17 non-neural enolase phosphopyruvate hydratase plasminogen-binding protein tau-crystallin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.23
Summary	This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011].
Disorder MIM:	172430
Disorder Html:	Enolase deficiency (1)

	LOC694593	XP_001083147.1
Bos taurus (cattle)	ENO1	NP_776474.2
Gallus gallus (chicken)	ENO1	NP_990451.1
Danio rerio (zebrafish)	eno1a	NP_997887.1
Caenorhabditis elegans (roundworm)	enol-1	NP_001022349.1
Saccharomyces cerevisiae (baker's yeast)	ERR1	NP_015038.1
Pan troglodytes (chimpanzee)	ENO1	NP_001207708.1
Mus musculus (house mouse)	Gm5506	NP_001020559.1
Homo sapiens (human)	ENO1	NP_001419.1
Mus musculus (house mouse)	Eno1	NP_075608.2
Saccharomyces cerevisiae (baker's yeast)	ERR3	NP_014056.3
Saccharomyces cerevisiae (baker's yeast)	ERR2	NP_015042.1
Arabidopsis thaliana (thale cress)	ENO1	NP_177543.1
Xenopus tropicalis (tropical clawed frog)	eno1	NP_989144.1
Rattus norvegicus (Norway rat)	Eno1	NP_036686.2
Drosophila melanogaster (fruit fly)	Eno	NP_722722.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007827	XP_317672.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ENO1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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