Eno cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eno
Entrez Gene ID	33351
Full Name	Enolase
Synonyms	BEST:LD15491,CG17654,CT32526,Dmel\CG17654,ENOA,Eno2,T18,anon-WO0153538.76,eno
General protein information	Preferred Names Enolase Names CG17654 gene product from transcript CG17654-RE CG17654-PA CG17654-PB CG17654-PC CG17654-PD CG17654-PE CG17654-PF Eno-PA Eno-PB Eno-PC Eno-PD Eno-PE Eno-PF enolase phosphopyruvate hydratase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 22B1-22B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_164434.3	NP_722724.1	enolase, isoform E
NM_164432.3	NP_722722.1	enolase, isoform C
NM_164433.3	NP_722723.1	enolase, isoform D
NM_058073.5	NP_477421.1	enolase, isoform A
NM_001169382.2	NP_001162853.1	enolase, isoform F
NM_164431.3	NP_722721.1	enolase, isoform B

Pathways from BioSystems

Homologies

	LOC694593	XP_001083147.1
Bos taurus (cattle)	ENO1	NP_776474.2
Gallus gallus (chicken)	ENO1	NP_990451.1
Danio rerio (zebrafish)	eno1a	NP_997887.1
Caenorhabditis elegans (roundworm)	enol-1	NP_001022349.1
Saccharomyces cerevisiae (baker's yeast)	ERR1	NP_015038.1
Pan troglodytes (chimpanzee)	ENO1	NP_001207708.1
Mus musculus (house mouse)	Gm5506	NP_001020559.1
Homo sapiens (human)	ENO1	NP_001419.1
Mus musculus (house mouse)	Eno1	NP_075608.2
Saccharomyces cerevisiae (baker's yeast)	ERR3	NP_014056.3
Saccharomyces cerevisiae (baker's yeast)	ERR2	NP_015042.1
Arabidopsis thaliana (thale cress)	ENO1	NP_177543.1
Xenopus tropicalis (tropical clawed frog)	eno1	NP_989144.1
Rattus norvegicus (Norway rat)	Eno1	NP_036686.2
Drosophila melanogaster (fruit fly)	Eno	NP_722722.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007827	XP_317672.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Crystal structure of enolase from Drosophila melanogaster has been resolved.
Title: Crystal structure of enolase from Drosophila melanogaster.

Systems biology approach reveals possible evolutionarily conserved moonlighting functions for enolase
Title: Systems biology approach reveals possible evolutionarily conserved moonlighting functions for enolase.

The following Eno gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eno cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13462	NM_058073.5 Latest version!	Drosophila melanogaster enolase, transcript variant A (Eno), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13462	NM_001169382.2 Latest version!	Drosophila melanogaster enolase, transcript variant F (Eno), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa16083	NM_164432.3 Latest version!	Drosophila melanogaster enolase, transcript variant C (Eno), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16083	NM_164431.3 Latest version!	Drosophila melanogaster enolase, transcript variant B (Eno), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16083	NM_164433.3 Latest version!	Drosophila melanogaster enolase, transcript variant D (Eno), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16083	NM_164434.3 Latest version!	Drosophila melanogaster enolase, transcript variant E (Eno), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eno ( NM_058073.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477421.1 Drosophila melanogaster enolase, transcript variant A (Eno), mRNA.

Price & Availability↑

CloneID	OFa13462
Clone ID Related Accession (Same CDS sequence)	NM_058073.5 , NM_001169382.2
Accession Version	NM_058073.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enolase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_058073.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCATCA AAGCGATCAA GGCCCGTCAA ATCTACGACT CCCGTGGCAA CCCCACCGTG 
GAGGTCGACC TGACCACCGA GCTGGGACTC TTCCGAGCCG CCGTGCCCTC CGGCGCCTCC 
ACTGGCGTCC ACGAGGCCCT GGAGCTGCGC GACAACGACA AGGCCAACTA CCATGGCAAG 
TCGGTGCTGA AGGCCGTGGG CCATGTGAAC GACACCCTGG GACCCGAGCT GATCAAGGCC 
AATCTGGATG TGGTTGACCA GGCGTCCATT GACAACTTCA TGATCAAGTT GGACGGCACC 
GAGAACAAGA GCAAGTTCGG AGCCAACGCC ATCCTGGGCG TTTCCCTGGC CGTGGCCAAG 
GCTGGAGCCG CCAAGAAGGG CGTGCCGCTG TACAAACACA TTGCCGATCT GGCTGGCAAC 
AAGGAGATCA TCCTGCCGGT GCCCGCTTTC AACGTGATCA ACGGCGGCAG CCACGCCGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTGCCCACCG GCGCCACCAG CTTCACAGAG 
GCCATGAAGA TGGGCTCCGA GGTGTACCAC CACCTGAAGA ATGTGATCAA GGCCAAGTTC 
GGTCTGGACG CCACCGCTGT GGGCGATGAG GGCGGCTTCG CCCCCAACAT CCAGTCCAAC 
AAGGAGGCTC TGAACCTGAT CAGCGATGCC ATCGCCAAGG CCGGATACAC CGGCAAGATC 
GAGATCGGCA TGGACGTGGC CGCCTCCGAG TTCTACAAGG ATGGCCAGTA CGATCTGGAC 
TTCAAGAACG AGAAGAGCGA CAAGTCGCAG TGGCTGCCCG CCGACAAGCT GGCCAACCTG 
TACCAGGAGT TTATTAAGGA CTTCCCCATC GTCTCGATTG AGGATCCCTT TGACCAGGAC 
CACTGGGAGG CCTGGTCCAA CCTGACCGGC TGCACCGACA TCCAGATCGT GGGCGATGAT 
CTGACCGTGA CCAACCCCAA GCGTATTGCC ACCGCCGTGG AGAAGAAGGC CTGCAACTGC 
CTGCTCCTGA AGGTCAACCA GATCGGCACC GTCACCGAGT CCATTGCCGC CCATCTGCTG 
GCCAAGAAGA ACGGCTGGGG CACCATGGTC TCCCACCGTT CCGGTGAGAC CGAGGACTCG 
TTCATCGGTG ACCTGGTCGT CGGTCTGTCC ACCGGCCAGA TCAAGACCGG AGCTCCATGC 
CGCTCGGAGC GTCTGGCCAA GTACAACCAG ATCCTGCGCA TCGAGGAGGA GATCGGCGCT 
GGCGTCAAGT TCGCCGGCAA GTCGTTCAGG AAGCCGCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477421.1
CDS	86..1387
Translation	MTIKAIKARQIYDSRGNPTVEVDLTTELGLFRAAVPSGASTGVHEALELRDNDKANYHGKSVLKAVGHVNDTLGPELIKANLDVVDQASIDNFMIKLDGTENKSKFGANAILGVSLAVAKAGAAKKGVPLYKHIADLAGNKEIILPVPAFNVINGGSHAGNKLAMQEFMILPTGATSFTEAMKMGSEVYHHLKNVIKAKFGLDATAVGDEGGFAPNIQSNKEALNLISDAIAKAGYTGKIEIGMDVAASEFYKDGQYDLDFKNEKSDKSQWLPADKLANLYQEFIKDFPIVSIEDPFDQDHWEAWSNLTGCTDIQIVGDDLTVTNPKRIATAVEKKACNCLLLKVNQIGTVTESIAAHLLAKKNGWGTMVSHRSGETEDSFIGDLVVGLSTGQIKTGAPCRSERLAKYNQILRIEEEIGAGVKFAGKSFRKPQ

Target ORF information:

RefSeq Version	NM_058073.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enolase, transcript variant A (Eno), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058073.5

ORF Insert Sequence:

ATGACCATCA AAGCGATCAA GGCCCGTCAA ATCTACGACT CCCGTGGCAA CCCCACCGTG 
GAGGTCGACC TGACCACCGA GCTGGGACTC TTCCGAGCCG CCGTGCCCTC CGGCGCCTCC 
ACTGGCGTCC ACGAGGCCCT GGAGCTGCGC GACAACGACA AGGCCAACTA CCATGGCAAG 
TCGGTGCTGA AGGCCGTGGG CCATGTGAAC GACACCCTGG GACCCGAGCT GATCAAGGCC 
AATCTGGATG TGGTTGACCA GGCGTCCATT GACAACTTCA TGATCAAGTT GGACGGCACC 
GAGAACAAGA GCAAGTTCGG AGCCAACGCC ATCCTGGGCG TTTCCCTGGC CGTGGCCAAG 
GCTGGAGCCG CCAAGAAGGG CGTGCCGCTG TACAAACACA TTGCCGATCT GGCTGGCAAC 
AAGGAGATCA TCCTGCCGGT GCCCGCTTTC AACGTGATCA ACGGCGGCAG CCACGCCGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTGCCCACCG GCGCCACCAG CTTCACAGAG 
GCCATGAAGA TGGGCTCCGA GGTGTACCAC CACCTGAAGA ATGTGATCAA GGCCAAGTTC 
GGTCTGGACG CCACCGCTGT GGGCGATGAG GGCGGCTTCG CCCCCAACAT CCAGTCCAAC 
AAGGAGGCTC TGAACCTGAT CAGCGATGCC ATCGCCAAGG CCGGATACAC CGGCAAGATC 
GAGATCGGCA TGGACGTGGC CGCCTCCGAG TTCTACAAGG ATGGCCAGTA CGATCTGGAC 
TTCAAGAACG AGAAGAGCGA CAAGTCGCAG TGGCTGCCCG CCGACAAGCT GGCCAACCTG 
TACCAGGAGT TTATTAAGGA CTTCCCCATC GTCTCGATTG AGGATCCCTT TGACCAGGAC 
CACTGGGAGG CCTGGTCCAA CCTGACCGGC TGCACCGACA TCCAGATCGT GGGCGATGAT 
CTGACCGTGA CCAACCCCAA GCGTATTGCC ACCGCCGTGG AGAAGAAGGC CTGCAACTGC 
CTGCTCCTGA AGGTCAACCA GATCGGCACC GTCACCGAGT CCATTGCCGC CCATCTGCTG 
GCCAAGAAGA ACGGCTGGGG CACCATGGTC TCCCACCGTT CCGGTGAGAC CGAGGACTCG 
TTCATCGGTG ACCTGGTCGT CGGTCTGTCC ACCGGCCAGA TCAAGACCGG AGCTCCATGC 
CGCTCGGAGC GTCTGGCCAA GTACAACCAG ATCCTGCGCA TCGAGGAGGA GATCGGCGCT 
GGCGTCAAGT TCGCCGGCAA GTCGTTCAGG AAGCCGCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eno ( NM_001169382.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162853.1 Drosophila melanogaster enolase, transcript variant F (Eno), mRNA.

Price & Availability↑

CloneID	OFa13462
Clone ID Related Accession (Same CDS sequence)	NM_058073.5 , NM_001169382.2
Accession Version	NM_001169382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enolase, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001169382.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCATCA AAGCGATCAA GGCCCGTCAA ATCTACGACT CCCGTGGCAA CCCCACCGTG 
GAGGTCGACC TGACCACCGA GCTGGGACTC TTCCGAGCCG CCGTGCCCTC CGGCGCCTCC 
ACTGGCGTCC ACGAGGCCCT GGAGCTGCGC GACAACGACA AGGCCAACTA CCATGGCAAG 
TCGGTGCTGA AGGCCGTGGG CCATGTGAAC GACACCCTGG GACCCGAGCT GATCAAGGCC 
AATCTGGATG TGGTTGACCA GGCGTCCATT GACAACTTCA TGATCAAGTT GGACGGCACC 
GAGAACAAGA GCAAGTTCGG AGCCAACGCC ATCCTGGGCG TTTCCCTGGC CGTGGCCAAG 
GCTGGAGCCG CCAAGAAGGG CGTGCCGCTG TACAAACACA TTGCCGATCT GGCTGGCAAC 
AAGGAGATCA TCCTGCCGGT GCCCGCTTTC AACGTGATCA ACGGCGGCAG CCACGCCGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTGCCCACCG GCGCCACCAG CTTCACAGAG 
GCCATGAAGA TGGGCTCCGA GGTGTACCAC CACCTGAAGA ATGTGATCAA GGCCAAGTTC 
GGTCTGGACG CCACCGCTGT GGGCGATGAG GGCGGCTTCG CCCCCAACAT CCAGTCCAAC 
AAGGAGGCTC TGAACCTGAT CAGCGATGCC ATCGCCAAGG CCGGATACAC CGGCAAGATC 
GAGATCGGCA TGGACGTGGC CGCCTCCGAG TTCTACAAGG ATGGCCAGTA CGATCTGGAC 
TTCAAGAACG AGAAGAGCGA CAAGTCGCAG TGGCTGCCCG CCGACAAGCT GGCCAACCTG 
TACCAGGAGT TTATTAAGGA CTTCCCCATC GTCTCGATTG AGGATCCCTT TGACCAGGAC 
CACTGGGAGG CCTGGTCCAA CCTGACCGGC TGCACCGACA TCCAGATCGT GGGCGATGAT 
CTGACCGTGA CCAACCCCAA GCGTATTGCC ACCGCCGTGG AGAAGAAGGC CTGCAACTGC 
CTGCTCCTGA AGGTCAACCA GATCGGCACC GTCACCGAGT CCATTGCCGC CCATCTGCTG 
GCCAAGAAGA ACGGCTGGGG CACCATGGTC TCCCACCGTT CCGGTGAGAC CGAGGACTCG 
TTCATCGGTG ACCTGGTCGT CGGTCTGTCC ACCGGCCAGA TCAAGACCGG AGCTCCATGC 
CGCTCGGAGC GTCTGGCCAA GTACAACCAG ATCCTGCGCA TCGAGGAGGA GATCGGCGCT 
GGCGTCAAGT TCGCCGGCAA GTCGTTCAGG AAGCCGCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162853.1
CDS	979..2280
Translation	MTIKAIKARQIYDSRGNPTVEVDLTTELGLFRAAVPSGASTGVHEALELRDNDKANYHGKSVLKAVGHVNDTLGPELIKANLDVVDQASIDNFMIKLDGTENKSKFGANAILGVSLAVAKAGAAKKGVPLYKHIADLAGNKEIILPVPAFNVINGGSHAGNKLAMQEFMILPTGATSFTEAMKMGSEVYHHLKNVIKAKFGLDATAVGDEGGFAPNIQSNKEALNLISDAIAKAGYTGKIEIGMDVAASEFYKDGQYDLDFKNEKSDKSQWLPADKLANLYQEFIKDFPIVSIEDPFDQDHWEAWSNLTGCTDIQIVGDDLTVTNPKRIATAVEKKACNCLLLKVNQIGTVTESIAAHLLAKKNGWGTMVSHRSGETEDSFIGDLVVGLSTGQIKTGAPCRSERLAKYNQILRIEEEIGAGVKFAGKSFRKPQ

Target ORF information:

RefSeq Version	NM_001169382.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enolase, transcript variant F (Eno), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169382.2

ORF Insert Sequence:

ATGACCATCA AAGCGATCAA GGCCCGTCAA ATCTACGACT CCCGTGGCAA CCCCACCGTG 
GAGGTCGACC TGACCACCGA GCTGGGACTC TTCCGAGCCG CCGTGCCCTC CGGCGCCTCC 
ACTGGCGTCC ACGAGGCCCT GGAGCTGCGC GACAACGACA AGGCCAACTA CCATGGCAAG 
TCGGTGCTGA AGGCCGTGGG CCATGTGAAC GACACCCTGG GACCCGAGCT GATCAAGGCC 
AATCTGGATG TGGTTGACCA GGCGTCCATT GACAACTTCA TGATCAAGTT GGACGGCACC 
GAGAACAAGA GCAAGTTCGG AGCCAACGCC ATCCTGGGCG TTTCCCTGGC CGTGGCCAAG 
GCTGGAGCCG CCAAGAAGGG CGTGCCGCTG TACAAACACA TTGCCGATCT GGCTGGCAAC 
AAGGAGATCA TCCTGCCGGT GCCCGCTTTC AACGTGATCA ACGGCGGCAG CCACGCCGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTGCCCACCG GCGCCACCAG CTTCACAGAG 
GCCATGAAGA TGGGCTCCGA GGTGTACCAC CACCTGAAGA ATGTGATCAA GGCCAAGTTC 
GGTCTGGACG CCACCGCTGT GGGCGATGAG GGCGGCTTCG CCCCCAACAT CCAGTCCAAC 
AAGGAGGCTC TGAACCTGAT CAGCGATGCC ATCGCCAAGG CCGGATACAC CGGCAAGATC 
GAGATCGGCA TGGACGTGGC CGCCTCCGAG TTCTACAAGG ATGGCCAGTA CGATCTGGAC 
TTCAAGAACG AGAAGAGCGA CAAGTCGCAG TGGCTGCCCG CCGACAAGCT GGCCAACCTG 
TACCAGGAGT TTATTAAGGA CTTCCCCATC GTCTCGATTG AGGATCCCTT TGACCAGGAC 
CACTGGGAGG CCTGGTCCAA CCTGACCGGC TGCACCGACA TCCAGATCGT GGGCGATGAT 
CTGACCGTGA CCAACCCCAA GCGTATTGCC ACCGCCGTGG AGAAGAAGGC CTGCAACTGC 
CTGCTCCTGA AGGTCAACCA GATCGGCACC GTCACCGAGT CCATTGCCGC CCATCTGCTG 
GCCAAGAAGA ACGGCTGGGG CACCATGGTC TCCCACCGTT CCGGTGAGAC CGAGGACTCG 
TTCATCGGTG ACCTGGTCGT CGGTCTGTCC ACCGGCCAGA TCAAGACCGG AGCTCCATGC 
CGCTCGGAGC GTCTGGCCAA GTACAACCAG ATCCTGCGCA TCGAGGAGGA GATCGGCGCT 
GGCGTCAAGT TCGCCGGCAA GTCGTTCAGG AAGCCGCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eno ( NM_164434.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722724.1 Drosophila melanogaster enolase, transcript variant E (Eno), mRNA.

Price & Availability↑

CloneID	OFa16083
Clone ID Related Accession (Same CDS sequence)	NM_164434.3 , NM_164432.3 , NM_164433.3 , NM_164431.3
Accession Version	NM_164434.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enolase, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164434.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTGGA CCGCTTCAGT TTTCTTGAGG ACTTCCACCA CTAGCATGAA GTTTCTGCGT 
CTGCGCTGGC CTCTCCCCAG AATTCCCCAG AATAAATCGG CAAACGTTGC GCCGCGTTTC 
AGAAGCAAAT CAGCAGTCAG CCAGCTCTCA TCTGGATTTA AATTTGTGCA GATCCGCAAA 
AGTACTTGTG ACAGCAACGA GATGACCATC AAAGCGATCA AGGCCCGTCA AATCTACGAC 
TCCCGTGGCA ACCCCACCGT GGAGGTCGAC CTGACCACCG AGCTGGGACT CTTCCGAGCC 
GCCGTGCCCT CCGGCGCCTC CACTGGCGTC CACGAGGCCC TGGAGCTGCG CGACAACGAC 
AAGGCCAACT ACCATGGCAA GTCGGTGCTG AAGGCCGTGG GCCATGTGAA CGACACCCTG 
GGACCCGAGC TGATCAAGGC CAATCTGGAT GTGGTTGACC AGGCGTCCAT TGACAACTTC 
ATGATCAAGT TGGACGGCAC CGAGAACAAG AGCAAGTTCG GAGCCAACGC CATCCTGGGC 
GTTTCCCTGG CCGTGGCCAA GGCTGGAGCC GCCAAGAAGG GCGTGCCGCT GTACAAACAC 
ATTGCCGATC TGGCTGGCAA CAAGGAGATC ATCCTGCCGG TGCCCGCTTT CAACGTGATC 
AACGGCGGCA GCCACGCCGG CAACAAGCTG GCCATGCAGG AGTTCATGAT CCTGCCCACC 
GGCGCCACCA GCTTCACAGA GGCCATGAAG ATGGGCTCCG AGGTGTACCA CCACCTGAAG 
AATGTGATCA AGGCCAAGTT CGGTCTGGAC GCCACCGCTG TGGGCGATGA GGGCGGCTTC 
GCCCCCAACA TCCAGTCCAA CAAGGAGGCT CTGAACCTGA TCAGCGATGC CATCGCCAAG 
GCCGGATACA CCGGCAAGAT CGAGATCGGC ATGGACGTGG CCGCCTCCGA GTTCTACAAG 
GATGGCCAGT ACGATCTGGA CTTCAAGAAC GAGAAGAGCG ACAAGTCGCA GTGGCTGCCC 
GCCGACAAGC TGGCCAACCT GTACCAGGAG TTTATTAAGG ACTTCCCCAT CGTCTCGATT 
GAGGATCCCT TTGACCAGGA CCACTGGGAG GCCTGGTCCA ACCTGACCGG CTGCACCGAC 
ATCCAGATCG TGGGCGATGA TCTGACCGTG ACCAACCCCA AGCGTATTGC CACCGCCGTG 
GAGAAGAAGG CCTGCAACTG CCTGCTCCTG AAGGTCAACC AGATCGGCAC CGTCACCGAG 
TCCATTGCCG CCCATCTGCT GGCCAAGAAG AACGGCTGGG GCACCATGGT CTCCCACCGT 
TCCGGTGAGA CCGAGGACTC GTTCATCGGT GACCTGGTCG TCGGTCTGTC CACCGGCCAG 
ATCAAGACCG GAGCTCCATG CCGCTCGGAG CGTCTGGCCA AGTACAACCA GATCCTGCGC 
ATCGAGGAGG AGATCGGCGC TGGCGTCAAG TTCGCCGGCA AGTCGTTCAG GAAGCCGCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722724.1
CDS	589..2091
Translation	MSWTASVFLRTSTTSMKFLRLRWPLPRIPQNKSANVAPRFRSKSAVSQLSSGFKFVQIRKSTCDSNEMTIKAIKARQIYDSRGNPTVEVDLTTELGLFRAAVPSGASTGVHEALELRDNDKANYHGKSVLKAVGHVNDTLGPELIKANLDVVDQASIDNFMIKLDGTENKSKFGANAILGVSLAVAKAGAAKKGVPLYKHIADLAGNKEIILPVPAFNVINGGSHAGNKLAMQEFMILPTGATSFTEAMKMGSEVYHHLKNVIKAKFGLDATAVGDEGGFAPNIQSNKEALNLISDAIAKAGYTGKIEIGMDVAASEFYKDGQYDLDFKNEKSDKSQWLPADKLANLYQEFIKDFPIVSIEDPFDQDHWEAWSNLTGCTDIQIVGDDLTVTNPKRIATAVEKKACNCLLLKVNQIGTVTESIAAHLLAKKNGWGTMVSHRSGETEDSFIGDLVVGLSTGQIKTGAPCRSERLAKYNQILRIEEEIGAGVKFAGKSFRKPQ

Target ORF information:

RefSeq Version	NM_164434.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enolase, transcript variant E (Eno), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164434.3

ORF Insert Sequence:

ATGTCTTGGA CCGCTTCAGT TTTCTTGAGG ACTTCCACCA CTAGCATGAA GTTTCTGCGT 
CTGCGCTGGC CTCTCCCCAG AATTCCCCAG AATAAATCGG CAAACGTTGC GCCGCGTTTC 
AGAAGCAAAT CAGCAGTCAG CCAGCTCTCA TCTGGATTTA AATTTGTGCA GATCCGCAAA 
AGTACTTGTG ACAGCAACGA GATGACCATC AAAGCGATCA AGGCCCGTCA AATCTACGAC 
TCCCGTGGCA ACCCCACCGT GGAGGTCGAC CTGACCACCG AGCTGGGACT CTTCCGAGCC 
GCCGTGCCCT CCGGCGCCTC CACTGGCGTC CACGAGGCCC TGGAGCTGCG CGACAACGAC 
AAGGCCAACT ACCATGGCAA GTCGGTGCTG AAGGCCGTGG GCCATGTGAA CGACACCCTG 
GGACCCGAGC TGATCAAGGC CAATCTGGAT GTGGTTGACC AGGCGTCCAT TGACAACTTC 
ATGATCAAGT TGGACGGCAC CGAGAACAAG AGCAAGTTCG GAGCCAACGC CATCCTGGGC 
GTTTCCCTGG CCGTGGCCAA GGCTGGAGCC GCCAAGAAGG GCGTGCCGCT GTACAAACAC 
ATTGCCGATC TGGCTGGCAA CAAGGAGATC ATCCTGCCGG TGCCCGCTTT CAACGTGATC 
AACGGCGGCA GCCACGCCGG CAACAAGCTG GCCATGCAGG AGTTCATGAT CCTGCCCACC 
GGCGCCACCA GCTTCACAGA GGCCATGAAG ATGGGCTCCG AGGTGTACCA CCACCTGAAG 
AATGTGATCA AGGCCAAGTT CGGTCTGGAC GCCACCGCTG TGGGCGATGA GGGCGGCTTC 
GCCCCCAACA TCCAGTCCAA CAAGGAGGCT CTGAACCTGA TCAGCGATGC CATCGCCAAG 
GCCGGATACA CCGGCAAGAT CGAGATCGGC ATGGACGTGG CCGCCTCCGA GTTCTACAAG 
GATGGCCAGT ACGATCTGGA CTTCAAGAAC GAGAAGAGCG ACAAGTCGCA GTGGCTGCCC 
GCCGACAAGC TGGCCAACCT GTACCAGGAG TTTATTAAGG ACTTCCCCAT CGTCTCGATT 
GAGGATCCCT TTGACCAGGA CCACTGGGAG GCCTGGTCCA ACCTGACCGG CTGCACCGAC 
ATCCAGATCG TGGGCGATGA TCTGACCGTG ACCAACCCCA AGCGTATTGC CACCGCCGTG 
GAGAAGAAGG CCTGCAACTG CCTGCTCCTG AAGGTCAACC AGATCGGCAC CGTCACCGAG 
TCCATTGCCG CCCATCTGCT GGCCAAGAAG AACGGCTGGG GCACCATGGT CTCCCACCGT 
TCCGGTGAGA CCGAGGACTC GTTCATCGGT GACCTGGTCG TCGGTCTGTC CACCGGCCAG 
ATCAAGACCG GAGCTCCATG CCGCTCGGAG CGTCTGGCCA AGTACAACCA GATCCTGCGC 
ATCGAGGAGG AGATCGGCGC TGGCGTCAAG TTCGCCGGCA AGTCGTTCAG GAAGCCGCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eno ( NM_164432.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722722.1 Drosophila melanogaster enolase, transcript variant C (Eno), mRNA.

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CloneID	OFa16083
Clone ID Related Accession (Same CDS sequence)	NM_164434.3 , NM_164432.3 , NM_164433.3 , NM_164431.3
Accession Version	NM_164432.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enolase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164432.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTGGA CCGCTTCAGT TTTCTTGAGG ACTTCCACCA CTAGCATGAA GTTTCTGCGT 
CTGCGCTGGC CTCTCCCCAG AATTCCCCAG AATAAATCGG CAAACGTTGC GCCGCGTTTC 
AGAAGCAAAT CAGCAGTCAG CCAGCTCTCA TCTGGATTTA AATTTGTGCA GATCCGCAAA 
AGTACTTGTG ACAGCAACGA GATGACCATC AAAGCGATCA AGGCCCGTCA AATCTACGAC 
TCCCGTGGCA ACCCCACCGT GGAGGTCGAC CTGACCACCG AGCTGGGACT CTTCCGAGCC 
GCCGTGCCCT CCGGCGCCTC CACTGGCGTC CACGAGGCCC TGGAGCTGCG CGACAACGAC 
AAGGCCAACT ACCATGGCAA GTCGGTGCTG AAGGCCGTGG GCCATGTGAA CGACACCCTG 
GGACCCGAGC TGATCAAGGC CAATCTGGAT GTGGTTGACC AGGCGTCCAT TGACAACTTC 
ATGATCAAGT TGGACGGCAC CGAGAACAAG AGCAAGTTCG GAGCCAACGC CATCCTGGGC 
GTTTCCCTGG CCGTGGCCAA GGCTGGAGCC GCCAAGAAGG GCGTGCCGCT GTACAAACAC 
ATTGCCGATC TGGCTGGCAA CAAGGAGATC ATCCTGCCGG TGCCCGCTTT CAACGTGATC 
AACGGCGGCA GCCACGCCGG CAACAAGCTG GCCATGCAGG AGTTCATGAT CCTGCCCACC 
GGCGCCACCA GCTTCACAGA GGCCATGAAG ATGGGCTCCG AGGTGTACCA CCACCTGAAG 
AATGTGATCA AGGCCAAGTT CGGTCTGGAC GCCACCGCTG TGGGCGATGA GGGCGGCTTC 
GCCCCCAACA TCCAGTCCAA CAAGGAGGCT CTGAACCTGA TCAGCGATGC CATCGCCAAG 
GCCGGATACA CCGGCAAGAT CGAGATCGGC ATGGACGTGG CCGCCTCCGA GTTCTACAAG 
GATGGCCAGT ACGATCTGGA CTTCAAGAAC GAGAAGAGCG ACAAGTCGCA GTGGCTGCCC 
GCCGACAAGC TGGCCAACCT GTACCAGGAG TTTATTAAGG ACTTCCCCAT CGTCTCGATT 
GAGGATCCCT TTGACCAGGA CCACTGGGAG GCCTGGTCCA ACCTGACCGG CTGCACCGAC 
ATCCAGATCG TGGGCGATGA TCTGACCGTG ACCAACCCCA AGCGTATTGC CACCGCCGTG 
GAGAAGAAGG CCTGCAACTG CCTGCTCCTG AAGGTCAACC AGATCGGCAC CGTCACCGAG 
TCCATTGCCG CCCATCTGCT GGCCAAGAAG AACGGCTGGG GCACCATGGT CTCCCACCGT 
TCCGGTGAGA CCGAGGACTC GTTCATCGGT GACCTGGTCG TCGGTCTGTC CACCGGCCAG 
ATCAAGACCG GAGCTCCATG CCGCTCGGAG CGTCTGGCCA AGTACAACCA GATCCTGCGC 
ATCGAGGAGG AGATCGGCGC TGGCGTCAAG TTCGCCGGCA AGTCGTTCAG GAAGCCGCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722722.1
CDS	388..1890
Translation	MSWTASVFLRTSTTSMKFLRLRWPLPRIPQNKSANVAPRFRSKSAVSQLSSGFKFVQIRKSTCDSNEMTIKAIKARQIYDSRGNPTVEVDLTTELGLFRAAVPSGASTGVHEALELRDNDKANYHGKSVLKAVGHVNDTLGPELIKANLDVVDQASIDNFMIKLDGTENKSKFGANAILGVSLAVAKAGAAKKGVPLYKHIADLAGNKEIILPVPAFNVINGGSHAGNKLAMQEFMILPTGATSFTEAMKMGSEVYHHLKNVIKAKFGLDATAVGDEGGFAPNIQSNKEALNLISDAIAKAGYTGKIEIGMDVAASEFYKDGQYDLDFKNEKSDKSQWLPADKLANLYQEFIKDFPIVSIEDPFDQDHWEAWSNLTGCTDIQIVGDDLTVTNPKRIATAVEKKACNCLLLKVNQIGTVTESIAAHLLAKKNGWGTMVSHRSGETEDSFIGDLVVGLSTGQIKTGAPCRSERLAKYNQILRIEEEIGAGVKFAGKSFRKPQ

Target ORF information:

RefSeq Version	NM_164432.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enolase, transcript variant C (Eno), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164432.3

ORF Insert Sequence:

ATGTCTTGGA CCGCTTCAGT TTTCTTGAGG ACTTCCACCA CTAGCATGAA GTTTCTGCGT 
CTGCGCTGGC CTCTCCCCAG AATTCCCCAG AATAAATCGG CAAACGTTGC GCCGCGTTTC 
AGAAGCAAAT CAGCAGTCAG CCAGCTCTCA TCTGGATTTA AATTTGTGCA GATCCGCAAA 
AGTACTTGTG ACAGCAACGA GATGACCATC AAAGCGATCA AGGCCCGTCA AATCTACGAC 
TCCCGTGGCA ACCCCACCGT GGAGGTCGAC CTGACCACCG AGCTGGGACT CTTCCGAGCC 
GCCGTGCCCT CCGGCGCCTC CACTGGCGTC CACGAGGCCC TGGAGCTGCG CGACAACGAC 
AAGGCCAACT ACCATGGCAA GTCGGTGCTG AAGGCCGTGG GCCATGTGAA CGACACCCTG 
GGACCCGAGC TGATCAAGGC CAATCTGGAT GTGGTTGACC AGGCGTCCAT TGACAACTTC 
ATGATCAAGT TGGACGGCAC CGAGAACAAG AGCAAGTTCG GAGCCAACGC CATCCTGGGC 
GTTTCCCTGG CCGTGGCCAA GGCTGGAGCC GCCAAGAAGG GCGTGCCGCT GTACAAACAC 
ATTGCCGATC TGGCTGGCAA CAAGGAGATC ATCCTGCCGG TGCCCGCTTT CAACGTGATC 
AACGGCGGCA GCCACGCCGG CAACAAGCTG GCCATGCAGG AGTTCATGAT CCTGCCCACC 
GGCGCCACCA GCTTCACAGA GGCCATGAAG ATGGGCTCCG AGGTGTACCA CCACCTGAAG 
AATGTGATCA AGGCCAAGTT CGGTCTGGAC GCCACCGCTG TGGGCGATGA GGGCGGCTTC 
GCCCCCAACA TCCAGTCCAA CAAGGAGGCT CTGAACCTGA TCAGCGATGC CATCGCCAAG 
GCCGGATACA CCGGCAAGAT CGAGATCGGC ATGGACGTGG CCGCCTCCGA GTTCTACAAG 
GATGGCCAGT ACGATCTGGA CTTCAAGAAC GAGAAGAGCG ACAAGTCGCA GTGGCTGCCC 
GCCGACAAGC TGGCCAACCT GTACCAGGAG TTTATTAAGG ACTTCCCCAT CGTCTCGATT 
GAGGATCCCT TTGACCAGGA CCACTGGGAG GCCTGGTCCA ACCTGACCGG CTGCACCGAC 
ATCCAGATCG TGGGCGATGA TCTGACCGTG ACCAACCCCA AGCGTATTGC CACCGCCGTG 
GAGAAGAAGG CCTGCAACTG CCTGCTCCTG AAGGTCAACC AGATCGGCAC CGTCACCGAG 
TCCATTGCCG CCCATCTGCT GGCCAAGAAG AACGGCTGGG GCACCATGGT CTCCCACCGT 
TCCGGTGAGA CCGAGGACTC GTTCATCGGT GACCTGGTCG TCGGTCTGTC CACCGGCCAG 
ATCAAGACCG GAGCTCCATG CCGCTCGGAG CGTCTGGCCA AGTACAACCA GATCCTGCGC 
ATCGAGGAGG AGATCGGCGC TGGCGTCAAG TTCGCCGGCA AGTCGTTCAG GAAGCCGCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eno ( NM_164433.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722723.1 Drosophila melanogaster enolase, transcript variant D (Eno), mRNA.

Price & Availability↑

CloneID	OFa16083
Clone ID Related Accession (Same CDS sequence)	NM_164434.3 , NM_164432.3 , NM_164433.3 , NM_164431.3
Accession Version	NM_164433.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enolase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164433.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTGGA CCGCTTCAGT TTTCTTGAGG ACTTCCACCA CTAGCATGAA GTTTCTGCGT 
CTGCGCTGGC CTCTCCCCAG AATTCCCCAG AATAAATCGG CAAACGTTGC GCCGCGTTTC 
AGAAGCAAAT CAGCAGTCAG CCAGCTCTCA TCTGGATTTA AATTTGTGCA GATCCGCAAA 
AGTACTTGTG ACAGCAACGA GATGACCATC AAAGCGATCA AGGCCCGTCA AATCTACGAC 
TCCCGTGGCA ACCCCACCGT GGAGGTCGAC CTGACCACCG AGCTGGGACT CTTCCGAGCC 
GCCGTGCCCT CCGGCGCCTC CACTGGCGTC CACGAGGCCC TGGAGCTGCG CGACAACGAC 
AAGGCCAACT ACCATGGCAA GTCGGTGCTG AAGGCCGTGG GCCATGTGAA CGACACCCTG 
GGACCCGAGC TGATCAAGGC CAATCTGGAT GTGGTTGACC AGGCGTCCAT TGACAACTTC 
ATGATCAAGT TGGACGGCAC CGAGAACAAG AGCAAGTTCG GAGCCAACGC CATCCTGGGC 
GTTTCCCTGG CCGTGGCCAA GGCTGGAGCC GCCAAGAAGG GCGTGCCGCT GTACAAACAC 
ATTGCCGATC TGGCTGGCAA CAAGGAGATC ATCCTGCCGG TGCCCGCTTT CAACGTGATC 
AACGGCGGCA GCCACGCCGG CAACAAGCTG GCCATGCAGG AGTTCATGAT CCTGCCCACC 
GGCGCCACCA GCTTCACAGA GGCCATGAAG ATGGGCTCCG AGGTGTACCA CCACCTGAAG 
AATGTGATCA AGGCCAAGTT CGGTCTGGAC GCCACCGCTG TGGGCGATGA GGGCGGCTTC 
GCCCCCAACA TCCAGTCCAA CAAGGAGGCT CTGAACCTGA TCAGCGATGC CATCGCCAAG 
GCCGGATACA CCGGCAAGAT CGAGATCGGC ATGGACGTGG CCGCCTCCGA GTTCTACAAG 
GATGGCCAGT ACGATCTGGA CTTCAAGAAC GAGAAGAGCG ACAAGTCGCA GTGGCTGCCC 
GCCGACAAGC TGGCCAACCT GTACCAGGAG TTTATTAAGG ACTTCCCCAT CGTCTCGATT 
GAGGATCCCT TTGACCAGGA CCACTGGGAG GCCTGGTCCA ACCTGACCGG CTGCACCGAC 
ATCCAGATCG TGGGCGATGA TCTGACCGTG ACCAACCCCA AGCGTATTGC CACCGCCGTG 
GAGAAGAAGG CCTGCAACTG CCTGCTCCTG AAGGTCAACC AGATCGGCAC CGTCACCGAG 
TCCATTGCCG CCCATCTGCT GGCCAAGAAG AACGGCTGGG GCACCATGGT CTCCCACCGT 
TCCGGTGAGA CCGAGGACTC GTTCATCGGT GACCTGGTCG TCGGTCTGTC CACCGGCCAG 
ATCAAGACCG GAGCTCCATG CCGCTCGGAG CGTCTGGCCA AGTACAACCA GATCCTGCGC 
ATCGAGGAGG AGATCGGCGC TGGCGTCAAG TTCGCCGGCA AGTCGTTCAG GAAGCCGCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722723.1
CDS	329..1831
Translation	MSWTASVFLRTSTTSMKFLRLRWPLPRIPQNKSANVAPRFRSKSAVSQLSSGFKFVQIRKSTCDSNEMTIKAIKARQIYDSRGNPTVEVDLTTELGLFRAAVPSGASTGVHEALELRDNDKANYHGKSVLKAVGHVNDTLGPELIKANLDVVDQASIDNFMIKLDGTENKSKFGANAILGVSLAVAKAGAAKKGVPLYKHIADLAGNKEIILPVPAFNVINGGSHAGNKLAMQEFMILPTGATSFTEAMKMGSEVYHHLKNVIKAKFGLDATAVGDEGGFAPNIQSNKEALNLISDAIAKAGYTGKIEIGMDVAASEFYKDGQYDLDFKNEKSDKSQWLPADKLANLYQEFIKDFPIVSIEDPFDQDHWEAWSNLTGCTDIQIVGDDLTVTNPKRIATAVEKKACNCLLLKVNQIGTVTESIAAHLLAKKNGWGTMVSHRSGETEDSFIGDLVVGLSTGQIKTGAPCRSERLAKYNQILRIEEEIGAGVKFAGKSFRKPQ

Target ORF information:

RefSeq Version	NM_164433.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enolase, transcript variant D (Eno), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164433.3

ORF Insert Sequence:

ATGTCTTGGA CCGCTTCAGT TTTCTTGAGG ACTTCCACCA CTAGCATGAA GTTTCTGCGT 
CTGCGCTGGC CTCTCCCCAG AATTCCCCAG AATAAATCGG CAAACGTTGC GCCGCGTTTC 
AGAAGCAAAT CAGCAGTCAG CCAGCTCTCA TCTGGATTTA AATTTGTGCA GATCCGCAAA 
AGTACTTGTG ACAGCAACGA GATGACCATC AAAGCGATCA AGGCCCGTCA AATCTACGAC 
TCCCGTGGCA ACCCCACCGT GGAGGTCGAC CTGACCACCG AGCTGGGACT CTTCCGAGCC 
GCCGTGCCCT CCGGCGCCTC CACTGGCGTC CACGAGGCCC TGGAGCTGCG CGACAACGAC 
AAGGCCAACT ACCATGGCAA GTCGGTGCTG AAGGCCGTGG GCCATGTGAA CGACACCCTG 
GGACCCGAGC TGATCAAGGC CAATCTGGAT GTGGTTGACC AGGCGTCCAT TGACAACTTC 
ATGATCAAGT TGGACGGCAC CGAGAACAAG AGCAAGTTCG GAGCCAACGC CATCCTGGGC 
GTTTCCCTGG CCGTGGCCAA GGCTGGAGCC GCCAAGAAGG GCGTGCCGCT GTACAAACAC 
ATTGCCGATC TGGCTGGCAA CAAGGAGATC ATCCTGCCGG TGCCCGCTTT CAACGTGATC 
AACGGCGGCA GCCACGCCGG CAACAAGCTG GCCATGCAGG AGTTCATGAT CCTGCCCACC 
GGCGCCACCA GCTTCACAGA GGCCATGAAG ATGGGCTCCG AGGTGTACCA CCACCTGAAG 
AATGTGATCA AGGCCAAGTT CGGTCTGGAC GCCACCGCTG TGGGCGATGA GGGCGGCTTC 
GCCCCCAACA TCCAGTCCAA CAAGGAGGCT CTGAACCTGA TCAGCGATGC CATCGCCAAG 
GCCGGATACA CCGGCAAGAT CGAGATCGGC ATGGACGTGG CCGCCTCCGA GTTCTACAAG 
GATGGCCAGT ACGATCTGGA CTTCAAGAAC GAGAAGAGCG ACAAGTCGCA GTGGCTGCCC 
GCCGACAAGC TGGCCAACCT GTACCAGGAG TTTATTAAGG ACTTCCCCAT CGTCTCGATT 
GAGGATCCCT TTGACCAGGA CCACTGGGAG GCCTGGTCCA ACCTGACCGG CTGCACCGAC 
ATCCAGATCG TGGGCGATGA TCTGACCGTG ACCAACCCCA AGCGTATTGC CACCGCCGTG 
GAGAAGAAGG CCTGCAACTG CCTGCTCCTG AAGGTCAACC AGATCGGCAC CGTCACCGAG 
TCCATTGCCG CCCATCTGCT GGCCAAGAAG AACGGCTGGG GCACCATGGT CTCCCACCGT 
TCCGGTGAGA CCGAGGACTC GTTCATCGGT GACCTGGTCG TCGGTCTGTC CACCGGCCAG 
ATCAAGACCG GAGCTCCATG CCGCTCGGAG CGTCTGGCCA AGTACAACCA GATCCTGCGC 
ATCGAGGAGG AGATCGGCGC TGGCGTCAAG TTCGCCGGCA AGTCGTTCAG GAAGCCGCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eno ( NM_164431.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722721.1 Drosophila melanogaster enolase, transcript variant B (Eno), mRNA.

Price & Availability↑

CloneID	OFa16083
Clone ID Related Accession (Same CDS sequence)	NM_164434.3 , NM_164432.3 , NM_164433.3 , NM_164431.3
Accession Version	NM_164431.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enolase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164431.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTTGGA CCGCTTCAGT TTTCTTGAGG ACTTCCACCA CTAGCATGAA GTTTCTGCGT 
CTGCGCTGGC CTCTCCCCAG AATTCCCCAG AATAAATCGG CAAACGTTGC GCCGCGTTTC 
AGAAGCAAAT CAGCAGTCAG CCAGCTCTCA TCTGGATTTA AATTTGTGCA GATCCGCAAA 
AGTACTTGTG ACAGCAACGA GATGACCATC AAAGCGATCA AGGCCCGTCA AATCTACGAC 
TCCCGTGGCA ACCCCACCGT GGAGGTCGAC CTGACCACCG AGCTGGGACT CTTCCGAGCC 
GCCGTGCCCT CCGGCGCCTC CACTGGCGTC CACGAGGCCC TGGAGCTGCG CGACAACGAC 
AAGGCCAACT ACCATGGCAA GTCGGTGCTG AAGGCCGTGG GCCATGTGAA CGACACCCTG 
GGACCCGAGC TGATCAAGGC CAATCTGGAT GTGGTTGACC AGGCGTCCAT TGACAACTTC 
ATGATCAAGT TGGACGGCAC CGAGAACAAG AGCAAGTTCG GAGCCAACGC CATCCTGGGC 
GTTTCCCTGG CCGTGGCCAA GGCTGGAGCC GCCAAGAAGG GCGTGCCGCT GTACAAACAC 
ATTGCCGATC TGGCTGGCAA CAAGGAGATC ATCCTGCCGG TGCCCGCTTT CAACGTGATC 
AACGGCGGCA GCCACGCCGG CAACAAGCTG GCCATGCAGG AGTTCATGAT CCTGCCCACC 
GGCGCCACCA GCTTCACAGA GGCCATGAAG ATGGGCTCCG AGGTGTACCA CCACCTGAAG 
AATGTGATCA AGGCCAAGTT CGGTCTGGAC GCCACCGCTG TGGGCGATGA GGGCGGCTTC 
GCCCCCAACA TCCAGTCCAA CAAGGAGGCT CTGAACCTGA TCAGCGATGC CATCGCCAAG 
GCCGGATACA CCGGCAAGAT CGAGATCGGC ATGGACGTGG CCGCCTCCGA GTTCTACAAG 
GATGGCCAGT ACGATCTGGA CTTCAAGAAC GAGAAGAGCG ACAAGTCGCA GTGGCTGCCC 
GCCGACAAGC TGGCCAACCT GTACCAGGAG TTTATTAAGG ACTTCCCCAT CGTCTCGATT 
GAGGATCCCT TTGACCAGGA CCACTGGGAG GCCTGGTCCA ACCTGACCGG CTGCACCGAC 
ATCCAGATCG TGGGCGATGA TCTGACCGTG ACCAACCCCA AGCGTATTGC CACCGCCGTG 
GAGAAGAAGG CCTGCAACTG CCTGCTCCTG AAGGTCAACC AGATCGGCAC CGTCACCGAG 
TCCATTGCCG CCCATCTGCT GGCCAAGAAG AACGGCTGGG GCACCATGGT CTCCCACCGT 
TCCGGTGAGA CCGAGGACTC GTTCATCGGT GACCTGGTCG TCGGTCTGTC CACCGGCCAG 
ATCAAGACCG GAGCTCCATG CCGCTCGGAG CGTCTGGCCA AGTACAACCA GATCCTGCGC 
ATCGAGGAGG AGATCGGCGC TGGCGTCAAG TTCGCCGGCA AGTCGTTCAG GAAGCCGCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722721.1
CDS	314..1816
Translation	MSWTASVFLRTSTTSMKFLRLRWPLPRIPQNKSANVAPRFRSKSAVSQLSSGFKFVQIRKSTCDSNEMTIKAIKARQIYDSRGNPTVEVDLTTELGLFRAAVPSGASTGVHEALELRDNDKANYHGKSVLKAVGHVNDTLGPELIKANLDVVDQASIDNFMIKLDGTENKSKFGANAILGVSLAVAKAGAAKKGVPLYKHIADLAGNKEIILPVPAFNVINGGSHAGNKLAMQEFMILPTGATSFTEAMKMGSEVYHHLKNVIKAKFGLDATAVGDEGGFAPNIQSNKEALNLISDAIAKAGYTGKIEIGMDVAASEFYKDGQYDLDFKNEKSDKSQWLPADKLANLYQEFIKDFPIVSIEDPFDQDHWEAWSNLTGCTDIQIVGDDLTVTNPKRIATAVEKKACNCLLLKVNQIGTVTESIAAHLLAKKNGWGTMVSHRSGETEDSFIGDLVVGLSTGQIKTGAPCRSERLAKYNQILRIEEEIGAGVKFAGKSFRKPQ

Target ORF information:

RefSeq Version	NM_164431.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enolase, transcript variant B (Eno), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164431.3

ORF Insert Sequence:

ATGTCTTGGA CCGCTTCAGT TTTCTTGAGG ACTTCCACCA CTAGCATGAA GTTTCTGCGT 
CTGCGCTGGC CTCTCCCCAG AATTCCCCAG AATAAATCGG CAAACGTTGC GCCGCGTTTC 
AGAAGCAAAT CAGCAGTCAG CCAGCTCTCA TCTGGATTTA AATTTGTGCA GATCCGCAAA 
AGTACTTGTG ACAGCAACGA GATGACCATC AAAGCGATCA AGGCCCGTCA AATCTACGAC 
TCCCGTGGCA ACCCCACCGT GGAGGTCGAC CTGACCACCG AGCTGGGACT CTTCCGAGCC 
GCCGTGCCCT CCGGCGCCTC CACTGGCGTC CACGAGGCCC TGGAGCTGCG CGACAACGAC 
AAGGCCAACT ACCATGGCAA GTCGGTGCTG AAGGCCGTGG GCCATGTGAA CGACACCCTG 
GGACCCGAGC TGATCAAGGC CAATCTGGAT GTGGTTGACC AGGCGTCCAT TGACAACTTC 
ATGATCAAGT TGGACGGCAC CGAGAACAAG AGCAAGTTCG GAGCCAACGC CATCCTGGGC 
GTTTCCCTGG CCGTGGCCAA GGCTGGAGCC GCCAAGAAGG GCGTGCCGCT GTACAAACAC 
ATTGCCGATC TGGCTGGCAA CAAGGAGATC ATCCTGCCGG TGCCCGCTTT CAACGTGATC 
AACGGCGGCA GCCACGCCGG CAACAAGCTG GCCATGCAGG AGTTCATGAT CCTGCCCACC 
GGCGCCACCA GCTTCACAGA GGCCATGAAG ATGGGCTCCG AGGTGTACCA CCACCTGAAG 
AATGTGATCA AGGCCAAGTT CGGTCTGGAC GCCACCGCTG TGGGCGATGA GGGCGGCTTC 
GCCCCCAACA TCCAGTCCAA CAAGGAGGCT CTGAACCTGA TCAGCGATGC CATCGCCAAG 
GCCGGATACA CCGGCAAGAT CGAGATCGGC ATGGACGTGG CCGCCTCCGA GTTCTACAAG 
GATGGCCAGT ACGATCTGGA CTTCAAGAAC GAGAAGAGCG ACAAGTCGCA GTGGCTGCCC 
GCCGACAAGC TGGCCAACCT GTACCAGGAG TTTATTAAGG ACTTCCCCAT CGTCTCGATT 
GAGGATCCCT TTGACCAGGA CCACTGGGAG GCCTGGTCCA ACCTGACCGG CTGCACCGAC 
ATCCAGATCG TGGGCGATGA TCTGACCGTG ACCAACCCCA AGCGTATTGC CACCGCCGTG 
GAGAAGAAGG CCTGCAACTG CCTGCTCCTG AAGGTCAACC AGATCGGCAC CGTCACCGAG 
TCCATTGCCG CCCATCTGCT GGCCAAGAAG AACGGCTGGG GCACCATGGT CTCCCACCGT 
TCCGGTGAGA CCGAGGACTC GTTCATCGGT GACCTGGTCG TCGGTCTGTC CACCGGCCAG 
ATCAAGACCG GAGCTCCATG CCGCTCGGAG CGTCTGGCCA AGTACAACCA GATCCTGCGC 
ATCGAGGAGG AGATCGGCGC TGGCGTCAAG TTCGCCGGCA AGTCGTTCAG GAAGCCGCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Eno cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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