PLD6 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLD6
Entrez Gene ID	201164
Full Name	phospholipase D family member 6
Synonyms	ZUC
General protein information	Preferred Names phospholipase D family member 6 Names mitochondrial cardiolipin hydrolase PLD 6 choline phosphatase 6 mitoPLD mitochondrial phospholipase phosphatidylcholine-hydrolyzing phospholipase D6 phospholipase D6 protein zucchini homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p11.2
Disorder MIM:	614960

mRNA and Protein(s)

mRNA	Protein	Name
XM_017024310.1	XP_016879799.1	mitochondrial cardiolipin hydrolase isoform X1
NM_178836.3	NP_849158.2	mitochondrial cardiolipin hydrolase
XM_017024310.2	XP_016879799.2	mitochondrial cardiolipin hydrolase isoform X1
NM_178836.4	NP_849158.2	mitochondrial cardiolipin hydrolase

Pathways from BioSystems

Homologies

Homo sapiens (human)	PLD6	NP_849158.2
Rattus norvegicus (Norway rat)	Pld6	XP_220517.1
Pan troglodytes (chimpanzee)	PLD6	NP_001233390.1
	PLD6	XP_001092274.2
Canis lupus familiaris (dog)	PLD6	XP_005620227.1
Bos taurus (cattle)	PLD6	NP_001258919.1
Danio rerio (zebrafish)	pld6	NP_001082883.1
Xenopus tropicalis (tropical clawed frog)	pld6	NP_001016875.2

Gene Ontology
Bibliography

Related articles in PubMed

Measuring Phospholipase D Enzymatic Activity Through Biochemical and Imaging Methods.
Philip F, Ha EE, Seeliger MA, Frohman MA
Methods in enzymology583309-325(2017)

Mitoguardin Regulates Mitochondrial Fusion through MitoPLD and Is Required for Neuronal Homeostasis.
Zhang Y, Liu X, Bai J, Tian X, Zhao X, Liu W, Duan X, Shang W, Fan HY, Tong C
Molecular cell61(1)111-24(2016 Jan)

Phosphatidic acid (PA)-preferring phospholipase A1 regulates mitochondrial dynamics.
Baba T, Kashiwagi Y, Arimitsu N, Kogure T, Edo A, Maruyama T, Nakao K, Nakanishi H, Kinoshita M, Frohman MA, Yamamoto A, Tani K
The Journal of biological chemistry289(16)11497-511(2014 Apr)

Roles for the lipid-signaling enzyme MitoPLD in mitochondrial dynamics, piRNA biogenesis, and spermatogenesis.
Gao Q, Frohman MA
BMB reports45(1)7-13(2012 Jan)

piRNA-associated germline nuage formation and spermatogenesis require MitoPLD profusogenic mitochondrial-surface lipid signaling.
Huang H, Gao Q, Peng X, Choi SY, Sarma K, Ren H, Morris AJ, Frohman MA
Developmental cell20(3)376-87(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Propose that MIGA proteins promote mitochondrial fusion by regulating mitochondrial phospholipid metabolism via MitoPLD.
Title: Mitoguardin Regulates Mitochondrial Fusion through MitoPLD and Is Required for Neuronal Homeostasis.

MitoPLD performs a critical function in a pathway that creates a specialized form of RNAi required by developing spermatocytes to suppress transposon mobilization during meiosis.
Title: Roles for the lipid-signaling enzyme MitoPLD in mitochondrial dynamics, piRNA biogenesis, and spermatogenesis.

Data suggest that mitochondrial-surface phosphatidic acid generated by MitoPLD/Zuc recruits or activates nuage components critical for piRNA production.
Title: piRNA-associated germline nuage formation and spermatogenesis require MitoPLD profusogenic mitochondrial-surface lipid signaling.

PLD6, aka MitoPLD, encodes an N-terminal mitochondrial localization sequence, localizes to the outer mitochondrial surface, generates phosphatidic acid, a signaling lipid, and triggers trans-mitochondrial association; involved in mitochondrial fusion.
Title: A common lipid links Mfn-mediated mitochondrial fusion and SNARE-regulated exocytosis.

The following PLD6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLD6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01452	NM_178836.4 Latest version!	Homo sapiens phospholipase D family member 6 (PLD6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu104273	XM_017024310.2 Latest version!	Homo sapiens phospholipase D family member 6 (PLD6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu86662	XM_017024310.1 Latest version!	Homo sapiens phospholipase D family member 6 (PLD6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu01452	NM_178836.3	Homo sapiens phospholipase D family member 6 (PLD6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLD6 ( NM_178836.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_849158.2 Homo sapiens phospholipase D family member 6 (PLD6), mRNA.

Price & Availability↑

CloneID	OHu01452
Clone ID Related Accession (Same CDS sequence)	NM_178836.3 , NM_178836.4
Accession Version	NM_178836.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	mitochondrial cardiolipin hydrolase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC326979.1, BC031263.1, AK090899.1 and AC055811.20. On Dec 4, 2009 this sequence version replaced NM_178836.2. ##Evidence-Data-START## Transcript exon combination :: BC031263.1, SRR1803616.35603.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGACGGT TGAGTTGGCA GGTGGCGGCC GCGGCGGCTG TGGGCCTGGC TCTGACTCTG 
GAGGCGCTGC CTTGGGTGCT GCGCTGGCTG CGGTCCAGGC GGCGGCGGCC GCGGCGCGAG 
GCGCTGTTCT TCCCGTCTCA GGTGACCTGT ACCGAGGCCC TGCTGCGGGC TCCGGGCGCG 
GAGCTGGCCG AGCTCCCCGA GGGCTGCCCG TGCGGCCTGC CCCACGGCGA GAGCGCGCTA 
AGCCGCCTGC TGCGTGCCCT GCTGGCCGCC CGCGCCAGCC TGGATCTCTG CCTGTTCGCC 
TTCTCCAGCC CGCAGCTGGG CCGCGCCGTG CAGTTGCTGC ACCAGCGTGG GGTGCGAGTG 
CGGGTCGTCA CCGACTGCGA CTACATGGCC CTCAACGGCT CGCAAATCGG TCTGCTGCGC 
AAGGCAGGGA TCCAGGTCCG GCACGATCAA GACCCAGGCT ACATGCATCA CAAGTTTGCC 
ATCGTGGACA AGAGGGTGCT CATCACTGGC TCGCTCAACT GGACCACGCA AGCCATCCAG 
AACAACAGGG AGAATGTTCT CATCACGGAG GACGACGAGT ACGTGCGGCT TTTTCTGGAA 
GAATTTGAGC GCATCTGGGA ACAGTTTAAC CCTACAAAGT ATACCTTTTT CCCACCAAAG 
AAAAGTCACG GAAGCTGTGC CCCACCTGTC TCCAGAGCTG GAGGGAGATT GCTTTCATGG 
CACAGAACTT GCGGCACCTC CAGCGAAAGC CAAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849158.2
CDS	47..805
Translation	MGRLSWQVAAAAAVGLALTLEALPWVLRWLRSRRRRPRREALFFPSQVTCTEALLRAPGAELAELPEGCPCGLPHGESALSRLLRALLAARASLDLCLFAFSSPQLGRAVQLLHQRGVRVRVVTDCDYMALNGSQIGLLRKAGIQVRHDQDPGYMHHKFAIVDKRVLITGSLNWTTQAIQNNRENVLITEDDEYVRLFLEEFERIWEQFNPTKYTFFPPKKSHGSCAPPVSRAGGRLLSWHRTCGTSSESQT

Target ORF information:

RefSeq Version	NM_178836.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase D family member 6 (PLD6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178836.3

ORF Insert Sequence:

ATGGGACGGT TGAGTTGGCA GGTGGCGGCC GCGGCGGCTG TGGGCCTGGC TCTGACTCTG 
GAGGCGCTGC CTTGGGTGCT GCGCTGGCTG CGGTCCAGGC GGCGGCGGCC GCGGCGCGAG 
GCGCTGTTCT TCCCGTCTCA GGTGACCTGT ACCGAGGCCC TGCTGCGGGC TCCGGGCGCG 
GAGCTGGCCG AGCTCCCCGA GGGCTGCCCG TGCGGCCTGC CCCACGGCGA GAGCGCGCTA 
AGCCGCCTGC TGCGTGCCCT GCTGGCCGCC CGCGCCAGCC TGGATCTCTG CCTGTTCGCC 
TTCTCCAGCC CGCAGCTGGG CCGCGCCGTG CAGTTGCTGC ACCAGCGTGG GGTGCGAGTG 
CGGGTCGTCA CCGACTGCGA CTACATGGCC CTCAACGGCT CGCAAATCGG TCTGCTGCGC 
AAGGCAGGGA TCCAGGTCCG GCACGATCAA GACCCAGGCT ACATGCATCA CAAGTTTGCC 
ATCGTGGACA AGAGGGTGCT CATCACTGGC TCGCTCAACT GGACCACGCA AGCCATCCAG 
AACAACAGGG AGAATGTTCT CATCACGGAG GACGACGAGT ACGTGCGGCT TTTTCTGGAA 
GAATTTGAGC GCATCTGGGA ACAGTTTAAC CCTACAAAGT ATACCTTTTT CCCACCAAAG 
AAAAGTCACG GAAGCTGTGC CCCACCTGTC TCCAGAGCTG GAGGGAGATT GCTTTCATGG 
CACAGAACTT GCGGCACCTC CAGCGAAAGC CAAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLD6 ( NM_178836.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_849158.2 Homo sapiens phospholipase D family member 6 (PLD6), mRNA.

Price & Availability↑

CloneID	OHu01452
Clone ID Related Accession (Same CDS sequence)	NM_178836.3 , NM_178836.4
Accession Version	NM_178836.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	mitochondrial cardiolipin hydrolase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC055811.20, DC326979.1, BC031263.1 and AK090899.1. On Nov 22, 2018 this sequence version replaced NM_178836.3. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.92588.1, SRR1803616.63221.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology MANE Ensembl match :: ENST00000321560.4/ ENSP00000317177.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGACGGT TGAGTTGGCA GGTGGCGGCC GCGGCGGCTG TGGGCCTGGC TCTGACTCTG 
GAGGCGCTGC CTTGGGTGCT GCGCTGGCTG CGGTCCAGGC GGCGGCGGCC GCGGCGCGAG 
GCGCTGTTCT TCCCGTCTCA GGTGACCTGT ACCGAGGCCC TGCTGCGGGC TCCGGGCGCG 
GAGCTGGCCG AGCTCCCCGA GGGCTGCCCG TGCGGCCTGC CCCACGGCGA GAGCGCGCTA 
AGCCGCCTGC TGCGTGCCCT GCTGGCCGCC CGCGCCAGCC TGGATCTCTG CCTGTTCGCC 
TTCTCCAGCC CGCAGCTGGG CCGCGCCGTG CAGTTGCTGC ACCAGCGTGG GGTGCGAGTG 
CGGGTCGTCA CCGACTGCGA CTACATGGCC CTCAACGGCT CGCAAATCGG TCTGCTGCGC 
AAGGCAGGGA TCCAGGTCCG GCACGATCAA GACCCAGGCT ACATGCATCA CAAGTTTGCC 
ATCGTGGACA AGAGGGTGCT CATCACTGGC TCGCTCAACT GGACCACGCA AGCCATCCAG 
AACAACAGGG AGAATGTTCT CATCACGGAG GACGACGAGT ACGTGCGGCT TTTTCTGGAA 
GAATTTGAGC GCATCTGGGA ACAGTTTAAC CCTACAAAGT ATACCTTTTT CCCACCAAAG 
AAAAGTCACG GAAGCTGTGC CCCACCTGTC TCCAGAGCTG GAGGGAGATT GCTTTCATGG 
CACAGAACTT GCGGCACCTC CAGCGAAAGC CAAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849158.2
CDS	48..806
Translation	MGRLSWQVAAAAAVGLALTLEALPWVLRWLRSRRRRPRREALFFPSQVTCTEALLRAPGAELAELPEGCPCGLPHGESALSRLLRALLAARASLDLCLFAFSSPQLGRAVQLLHQRGVRVRVVTDCDYMALNGSQIGLLRKAGIQVRHDQDPGYMHHKFAIVDKRVLITGSLNWTTQAIQNNRENVLITEDDEYVRLFLEEFERIWEQFNPTKYTFFPPKKSHGSCAPPVSRAGGRLLSWHRTCGTSSESQT

Target ORF information:

RefSeq Version	NM_178836.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase D family member 6 (PLD6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178836.4

ORF Insert Sequence:

ATGGGACGGT TGAGTTGGCA GGTGGCGGCC GCGGCGGCTG TGGGCCTGGC TCTGACTCTG 
GAGGCGCTGC CTTGGGTGCT GCGCTGGCTG CGGTCCAGGC GGCGGCGGCC GCGGCGCGAG 
GCGCTGTTCT TCCCGTCTCA GGTGACCTGT ACCGAGGCCC TGCTGCGGGC TCCGGGCGCG 
GAGCTGGCCG AGCTCCCCGA GGGCTGCCCG TGCGGCCTGC CCCACGGCGA GAGCGCGCTA 
AGCCGCCTGC TGCGTGCCCT GCTGGCCGCC CGCGCCAGCC TGGATCTCTG CCTGTTCGCC 
TTCTCCAGCC CGCAGCTGGG CCGCGCCGTG CAGTTGCTGC ACCAGCGTGG GGTGCGAGTG 
CGGGTCGTCA CCGACTGCGA CTACATGGCC CTCAACGGCT CGCAAATCGG TCTGCTGCGC 
AAGGCAGGGA TCCAGGTCCG GCACGATCAA GACCCAGGCT ACATGCATCA CAAGTTTGCC 
ATCGTGGACA AGAGGGTGCT CATCACTGGC TCGCTCAACT GGACCACGCA AGCCATCCAG 
AACAACAGGG AGAATGTTCT CATCACGGAG GACGACGAGT ACGTGCGGCT TTTTCTGGAA 
GAATTTGAGC GCATCTGGGA ACAGTTTAAC CCTACAAAGT ATACCTTTTT CCCACCAAAG 
AAAAGTCACG GAAGCTGTGC CCCACCTGTC TCCAGAGCTG GAGGGAGATT GCTTTCATGG 
CACAGAACTT GCGGCACCTC CAGCGAAAGC CAAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLD6 ( XM_017024310.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879799.2 Homo sapiens phospholipase D family member 6 (PLD6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu104273
Clone ID Related Accession (Same CDS sequence)	XM_017024310.2
Accession Version	XM_017024310.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitochondrial cardiolipin hydrolase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017024310.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGGCT GGGCGTGGCC TGGCACATGG CCGCTGGCGG CGCGTGACGG GGGCGTGGCC 
TGGCGGTCTA GGGCTGGGGG CGCGCAGACT CCGCTGCGGC GGCGTGGCTG TGGGTCCCGG 
ATTAGCGGCG GCATGGGACG GTTGAGTTGG CAGGTGGCGG CCGCGGCGGC TGTGGGCCTG 
GCTCTGACTC TGGAGGCGCT GCCTTGGGTG CTGCGCTGGC TGCGGTCCAG GCGGCGGCGG 
CCGCGGCGCG AGGCGCTGTT CTTCCCGTCT CAGGTGACCT GTACCGAGGC CCTGCTGCGG 
GCTCCGGGCG CGGAGCTGGC CGAGCTCCCC GAGGGCTGCC CGTGCGGCCT GCCCCACGGC 
GAGAGCGCGC TAAGCCGCCT GCTGCGTGCC CTGCTGGCCG CCCGCGCCAG CCTGGATCTC 
TGCCTGTTCG CCTTCTCCAG CCCGCAGCTG GGCCGCGCCG TGCAGTTGCT GCACCAGCGT 
GGGGTGCGAG TGCGGGTCGT CACCGACTGC GACTACATGG CCCTCAACGG CTCGCAAATC 
GGTCTGCTGC GCAAGGGATC CAGGTCCGGC ACGATCAAGA CCCAGGCTAC ATGCATCACA 
AGTTTGCCAT CGTGGACAAG AGGGTGCTCA TCACTGGCTC GCTCAACTGG ACCACGCAAG 
CCATCCAGAA CAACAGGGAG AATGTTCTCA TCACGGAGGA CGACGAGTAC GTGCGGCTTT 
TTCTGGAAGA ATTTGAGCGC ATCTGGGAAC AGTTTAACCC TACAAAGTAT ACCTTTTTCC 
CACCAAAGAA AAGTCACGGA AGCTGTGCCC CACCTGTCTC CAGAGCTGGA GGGAGATTGC 
TTTCATGGCA CAGAACTTGC GGCACCTCCA GCGAAAGCCA AACCTAACCA AGAATGGGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879799.2
CDS	488..1390
Translation	MGGWAWPGTWPLAARDGGVAWRSRAGGAQTPLRRRGCGSRISGGMGRLSWQVAAAAAVGLALTLEALPWVLRWLRSRRRRPRREALFFPSQVTCTEALLRAPGAELAELPEGCPCGLPHGESALSRLLRALLAARASLDLCLFAFSSPQLGRAVQLLHQRGVRVRVVTDCDYMALNGSQIGLLRKGSRSGTIKTQATCITSLPSWTRGCSSLARSTGPRKPSRTTGRMFSSRRTTSTCGFFWKNLSASGNSLTLQSIPFSHQRKVTEAVPHLSPELEGDCFHGTELAAPPAKAKPNQEWG

Target ORF information:

RefSeq Version	XM_017024310.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase D family member 6 (PLD6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024310.2

ORF Insert Sequence:

ATGGGGGGCT GGGCGTGGCC TGGCACATGG CCGCTGGCGG CGCGTGACGG GGGCGTGGCC 
TGGCGGTCTA GGGCTGGGGG CGCGCAGACT CCGCTGCGGC GGCGTGGCTG TGGGTCCCGG 
ATTAGCGGCG GCATGGGACG GTTGAGTTGG CAGGTGGCGG CCGCGGCGGC TGTGGGCCTG 
GCTCTGACTC TGGAGGCGCT GCCTTGGGTG CTGCGCTGGC TGCGGTCCAG GCGGCGGCGG 
CCGCGGCGCG AGGCGCTGTT CTTCCCGTCT CAGGTGACCT GTACCGAGGC CCTGCTGCGG 
GCTCCGGGCG CGGAGCTGGC CGAGCTCCCC GAGGGCTGCC CGTGCGGCCT GCCCCACGGC 
GAGAGCGCGC TAAGCCGCCT GCTGCGTGCC CTGCTGGCCG CCCGCGCCAG CCTGGATCTC 
TGCCTGTTCG CCTTCTCCAG CCCGCAGCTG GGCCGCGCCG TGCAGTTGCT GCACCAGCGT 
GGGGTGCGAG TGCGGGTCGT CACCGACTGC GACTACATGG CCCTCAACGG CTCGCAAATC 
GGTCTGCTGC GCAAGGGATC CAGGTCCGGC ACGATCAAGA CCCAGGCTAC ATGCATCACA 
AGTTTGCCAT CGTGGACAAG AGGGTGCTCA TCACTGGCTC GCTCAACTGG ACCACGCAAG 
CCATCCAGAA CAACAGGGAG AATGTTCTCA TCACGGAGGA CGACGAGTAC GTGCGGCTTT 
TTCTGGAAGA ATTTGAGCGC ATCTGGGAAC AGTTTAACCC TACAAAGTAT ACCTTTTTCC 
CACCAAAGAA AAGTCACGGA AGCTGTGCCC CACCTGTCTC CAGAGCTGGA GGGAGATTGC 
TTTCATGGCA CAGAACTTGC GGCACCTCCA GCGAAAGCCA AACCTAACCA AGAATGGGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLD6 ( XM_017024310.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879799.1 Homo sapiens phospholipase D family member 6 (PLD6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu86662
Clone ID Related Accession (Same CDS sequence)	XM_017024310.1
Accession Version	XM_017024310.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mitochondrial cardiolipin hydrolase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACGGT TGAGTTGGCA GGTGGCGGCC GCGGCGGCTG TGGGCCTGGC TCTGACTCTG 
GAGGCGCTGC CTTGGGTGCT GCGCTGGCTG CGGTCCAGGC GGCGGCGGCC GCGGCGCGAG 
GCGCTGTTCT TCCCGTCTCA GGTGACCTGT ACCGAGGCCC TGCTGCGGGC TCCGGGCGCG 
GAGCTGGCCG AGCTCCCCGA GGGCTGCCCG TGCGGCCTGC CCCACGGCGA GAGCGCGCTA 
AGCCGCCTGC TGCGTGCCCT GCTGGCCGCC CGCGCCAGCC TGGATCTCTG CCTGTTCGCC 
TTCTCCAGCC CGCAGCTGGG CCGCGCCGTG CAGTTGCTGC ACCAGCGTGG GGTGCGAGTG 
CGGGTCGTCA CCGACTGCGA CTACATGGCC CTCAACGGCT CGCAAATCGG TCTGCTGCGC 
AAGGGATCCA GGTCCGGCAC GATCAAGACC CAGGCTACAT GCATCACAAG TTTGCCATCG 
TGGACAAGAG GGTGCTCATC ACTGGCTCGC TCAACTGGAC CACGCAAGCC ATCCAGAACA 
ACAGGGAGAA TGTTCTCATC ACGGAGGACG ACGAGTACGT GCGGCTTTTT CTGGAAGAAT 
TTGAGCGCAT CTGGGAACAG TTTAACCCTA CAAAGTATAC CTTTTTCCCA CCAAAGAAAA 
GTCACGGAAG CTGTGCCCCA CCTGTCTCCA GAGCTGGAGG GAGATTGCTT TCATGGCACA 
GAACTTGCGG CACCTCCAGC GAAAGCCAAA CCTAACCAAG AATGGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879799.1
CDS	47..817
Translation	MGRLSWQVAAAAAVGLALTLEALPWVLRWLRSRRRRPRREALFFPSQVTCTEALLRAPGAELAELPEGCPCGLPHGESALSRLLRALLAARASLDLCLFAFSSPQLGRAVQLLHQRGVRVRVVTDCDYMALNGSQIGLLRKGSRSGTIKTQATCITSLPSWTRGCSSLARSTGPRKPSRTTGRMFSSRRTTSTCGFFWKNLSASGNSLTLQSIPFSHQRKVTEAVPHLSPELEGDCFHGTELAAPPAKAKPNQEWG

Target ORF information:

RefSeq Version	XM_017024310.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase D family member 6 (PLD6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024310.1

ORF Insert Sequence:

ATGGGACGGT TGAGTTGGCA GGTGGCGGCC GCGGCGGCTG TGGGCCTGGC TCTGACTCTG 
GAGGCGCTGC CTTGGGTGCT GCGCTGGCTG CGGTCCAGGC GGCGGCGGCC GCGGCGCGAG 
GCGCTGTTCT TCCCGTCTCA GGTGACCTGT ACCGAGGCCC TGCTGCGGGC TCCGGGCGCG 
GAGCTGGCCG AGCTCCCCGA GGGCTGCCCG TGCGGCCTGC CCCACGGCGA GAGCGCGCTA 
AGCCGCCTGC TGCGTGCCCT GCTGGCCGCC CGCGCCAGCC TGGATCTCTG CCTGTTCGCC 
TTCTCCAGCC CGCAGCTGGG CCGCGCCGTG CAGTTGCTGC ACCAGCGTGG GGTGCGAGTG 
CGGGTCGTCA CCGACTGCGA CTACATGGCC CTCAACGGCT CGCAAATCGG TCTGCTGCGC 
AAGGGATCCA GGTCCGGCAC GATCAAGACC CAGGCTACAT GCATCACAAG TTTGCCATCG 
TGGACAAGAG GGTGCTCATC ACTGGCTCGC TCAACTGGAC CACGCAAGCC ATCCAGAACA 
ACAGGGAGAA TGTTCTCATC ACGGAGGACG ACGAGTACGT GCGGCTTTTT CTGGAAGAAT 
TTGAGCGCAT CTGGGAACAG TTTAACCCTA CAAAGTATAC CTTTTTCCCA CCAAAGAAAA 
GTCACGGAAG CTGTGCCCCA CCTGTCTCCA GAGCTGGAGG GAGATTGCTT TCATGGCACA 
GAACTTGCGG CACCTCCAGC GAAAGCCAAA CCTAACCAAG AATGGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Measuring Phospholipase D Enzymatic Activity Through Biochemical and Imaging Methods.
Methods in enzymology583309-325(2017)
Philip F,Ha EE,Seeliger MA,Frohman MA

Mitoguardin Regulates Mitochondrial Fusion through MitoPLD and Is Required for Neuronal Homeostasis.
Molecular cell61(1)111-24(2016 Jan)
Zhang Y,Liu X,Bai J,Tian X,Zhao X,Liu W,Duan X,Shang W,Fan HY,Tong C

Phosphatidic acid (PA)-preferring phospholipase A1 regulates mitochondrial dynamics.
The Journal of biological chemistry289(16)11497-511(2014 Apr)
Baba T,Kashiwagi Y,Arimitsu N,Kogure T,Edo A,Maruyama T,Nakao K,Nakanishi H,Kinoshita M,Frohman MA,Yamamoto A,Tani K

Roles for the lipid-signaling enzyme MitoPLD in mitochondrial dynamics, piRNA biogenesis, and spermatogenesis.
BMB reports45(1)7-13(2012 Jan)
Gao Q,Frohman MA

piRNA-associated germline nuage formation and spermatogenesis require MitoPLD profusogenic mitochondrial-surface lipid signaling.
Developmental cell20(3)376-87(2011 Mar)
Huang H,Gao Q,Peng X,Choi SY,Sarma K,Ren H,Morris AJ,Frohman MA

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PLD6 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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