KRT78 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	KRT78
Entrez Gene ID	196374
Full Name	keratin 78
Synonyms	CK-78,K5B,K78,Kb40
General protein information	Preferred Names keratin 78 Names keratin, type II cytoskeletal 78 cytokeratin-78 keratin 78, type II keratin-5b type-II keratin Kb40
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.13
Summary	This gene is a member of the type II keratin gene family and encodes a protein with an intermediate filament domain. Keratins are the major structural proteins in epithelial cells, forming a cytoplasmic network of 10 to 12 nm wide intermediate filaments and creating a scaffold that gives cells the ability to withstand mechanical and non-mechanical stresses. The genes of the type II keratin family are located as a gene cluster at 12p13.13. Four pseudogenes of this gene family have been identified. [provided by RefSeq, Jul 2008].
Disorder MIM:	611159

mRNA and Protein(s)

mRNA	Protein	Name
NM_173352.4	NP_775487.2	keratin, type II cytoskeletal 78 isoform 1
NM_173352.3	NP_775487.2	keratin, type II cytoskeletal 78 isoform 1
XM_011538010.1	XP_011536312.1	keratin, type II cytoskeletal 78 isoform X1
NM_001300814.1	NP_001287743.1	keratin, type II cytoskeletal 78 isoform 2

Pathways from BioSystems

Homologies

	LOC698145	XP_001086676.2
Canis lupus familiaris (dog)	KRT78	XP_543640.2
Homo sapiens (human)	KRT78	NP_775487.2
Rattus norvegicus (Norway rat)	Krt78	XP_002729895.2
Mus musculus (house mouse)	Krt78	NP_997652.4
Pan troglodytes (chimpanzee)	KRT78	XP_509082.2
Bos taurus (cattle)	KRT78	NP_001095547.1

Gene Ontology
Bibliography

Related articles in PubMed

Localisation of keratin K78 in the basal layer and first suprabasal layers of stratified epithelia completes expression catalogue of type II keratins and provides new insights into sequential keratin expression.
Langbein L, Eckhart L, Fischer H, Rogers MA, Praetzel-Wunder S, Parry DA, Kittstein W, Schweizer J
Cell and tissue research363(3)735-50(2016 Mar)

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
B?hm D, Keller K, Pieter J, Boehm N, Wolters D, Siggelkow W, Lebrecht A, Schmidt M, K?lbl H, Pfeiffer N, Grus FH
Oncology reports28(2)429-38(2012 Aug)

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal of proteome research8(3)1304-14(2009 Mar)

New consensus nomenclature for mammalian keratins.
Schweizer J, Bowden PE, Coulombe PA, Langbein L, Lane EB, Magin TM, Maltais L, Omary MB, Parry DA, Rogers MA, Wright MW
The Journal of cell biology174(2)169-74(2006 Jul)

Characterization of new members of the human type II keratin gene family and a general evaluation of the keratin gene domain on chromosome 12q13.13.
Rogers MA, Edler L, Winter H, Langbein L, Beckmann I, Schweizer J
The Journal of investigative dermatology124(3)536-44(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

K78 is strongly expressed in the basal and parabasal cell layers with decreasing intensity in the lower suprabasal cells of keratinising and non-keratinising squamous epithelia and keratinocyte cultures.
Title: Localisation of keratin K78 in the basal layer and first suprabasal layers of stratified epithelia completes expression catalogue of type II keratins and provides new insights into sequential keratin expression.

The following KRT78 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KRT78 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19538	NM_173352.4 Latest version!	Homo sapiens keratin 78 (KRT78), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
OHu46368	NM_001300814.1 Latest version!	Homo sapiens keratin 78 (KRT78), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu77478	XM_011538010.1 Latest version!	Homo sapiens keratin 78 (KRT78), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu19538	NM_173352.3	Homo sapiens keratin 78 (KRT78), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KRT78 ( NM_173352.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_775487.2 Homo sapiens keratin 78 (KRT78), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19538
Clone ID Related Accession (Same CDS sequence)	NM_173352.4 , NM_173352.3
Accession Version	NM_173352.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	keratin, type II cytoskeletal 78 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290901.1, BX647095.1 and AC107016.20. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_173352.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK290901.1, ERR279862.1360.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2156670 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000304620.5/ ENSP00000306261.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTCT CCCCATGCCG GGCCCAGAGG GGCTTCAGCG CTCGCTCAGC CTGTTCTGCT 
CGCTCAAGGG GCCGCAGCAG GGGAGGCTTC AGCAGCAGGG GCGGCTTCAG CAGCAGGAGC 
CTTAATTCCT TTGGGGGGTG CCTGGAAGGC TCTCGTGGGA GTACCTGGGG GTCAGGGGGT 
AGGCTGGGGG TGCGGTTTGG GGAGTGGAGT GGTGGGCCTG GGCTCTCCCT GTGCCCTCCG 
GGGGGCATCC AAGAAGTGAC CATCAACCAG AATCTGCTGA CCCCACTGAA GATTGAGATC 
GATCCCCAGT TCCAGGTGGT GCGGACGCAG GAGACCCAGG AGATCAGAAC CCTCAACAAC 
CAGTTTGCTT CCTTCATTGA CAAGGTGCGG TTCCTGGAGC AGCAGAACAA GGTCCTGGAG 
ACGAAGTGGC ATCTGCTGCA GCAACAGGGG TTGAGTGGCA GCCAGCAGGG CCTGGAGCCT 
GTCTTTGAGG CCTGCCTGGA TCAGCTCAGG AAGCAGCTGG AGCAGCTCCA GGGAGAACGA 
GGGGCTCTGG ATGCTGAGTT GAAGGCCTGC CGGGACCAGG AGGAGGAGTA TAAGTCCAAG 
TATGAGGAGG AGGCCCACAG GCGTGCCACA CTTGAGAACG ACTTTGTGGT CCTCAAGAAG 
GATGTGGATG GGGTTTTCCT GAGCAAGATG GAGTTGGAGG GCAAGCTGGA GGCTCTGAGA 
GAGTACCTCT ACTTCTTGAA GCATCTGAAT GAAGAAGAGC TGGGCCAGCT CCAGACCCAG 
GCCAGCGACA CGTCTGTGGT GCTGTCCATG GACAACAACC GCTACCTGGA CTTCAGCAGC 
ATCATCACTG AGGTCCGCGC CCGGTACGAG GAGATCGCCC GGAGCAGCAA GGCTGAGGCT 
GAGGCCTTGT ACCAGACCAA GTACCAGGAA CTTCAGGTGT CTGCCCAGCT TCATGGGGAC 
AGGATGCAGG AAACGAAAGT CCAGATCTCT CAGCTACACC AAGAGATTCA GAGGCTGCAG 
AGTCAGACTG AGAACCTCAA GAAGCAGAAC GCCAGCCTGC AGGCCGCCAT CACTGATGCT 
GAGCAGCGTG GGGAGCTGGC CCTCAAGGAC GCTCAGGCCA AGGTGGACGA GCTGGAGGCT 
GCTCTGAGGA TGGCCAAGCA GAACCTGGCC CGGCTGCTGT GCGAGTACCA GGAGCTGACG 
AGCACGAAGC TTTCCCTGGA TGTGGAGATT GCCACTTACC GCAGGCTGCT GGAGGGCGAG 
GAGTGCAGGA TGTCTGGGGA GTGCACCAGC CAGGTCACTA TCTCCTCGGT GGGAGGCAGC 
GCTGTCATGT CTGGAGGAGT TGGTGGAGGC TTGGGGAGCA CTTGTGGACT CGGTAGTGGG 
AAAGGCAGCC CTGGGTCCTG CTGCACCAGC ATTGTGACTG GAGGCTCCAA CATCATTCTG 
GGCTCTGGGA AGGACCCTGT TTTGGATTCC TGCTCTGTGT CTGGCTCCAG CGCTGGCTCC 
AGCTGCCACA CCATCCTGAA GAAGACAGTT GAGTCGAGTC TGAAGACATC CATCACCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775487.2
CDS	65..1627
Translation	MSLSPCRAQRGFSARSACSARSRGRSRGGFSSRGGFSSRSLNSFGGCLEGSRGSTWGSGGRLGVRFGEWSGGPGLSLCPPGGIQEVTINQNLLTPLKIEIDPQFQVVRTQETQEIRTLNNQFASFIDKVRFLEQQNKVLETKWHLLQQQGLSGSQQGLEPVFEACLDQLRKQLEQLQGERGALDAELKACRDQEEEYKSKYEEEAHRRATLENDFVVLKKDVDGVFLSKMELEGKLEALREYLYFLKHLNEEELGQLQTQASDTSVVLSMDNNRYLDFSSIITEVRARYEEIARSSKAEAEALYQTKYQELQVSAQLHGDRMQETKVQISQLHQEIQRLQSQTENLKKQNASLQAAITDAEQRGELALKDAQAKVDELEAALRMAKQNLARLLCEYQELTSTKLSLDVEIATYRRLLEGEECRMSGECTSQVTISSVGGSAVMSGGVGGGLGSTCGLGSGKGSPGSCCTSIVTGGSNIILGSGKDPVLDSCSVSGSSAGSSCHTILKKTVESSLKTSITY

Target ORF information:

RefSeq Version	NM_173352.4
Organism	Homo sapiens(human)
Definition	Homo sapiens keratin 78 (KRT78), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173352.4

ORF Insert Sequence:

ATGTCTCTCT CCCCATGCCG GGCCCAGAGG GGCTTCAGCG CTCGCTCAGC CTGTTCTGCT 
CGCTCAAGGG GCCGCAGCAG GGGAGGCTTC AGCAGCAGGG GCGGCTTCAG CAGCAGGAGC 
CTTAATTCCT TTGGGGGGTG CCTGGAAGGC TCTCGTGGGA GTACCTGGGG GTCAGGGGGT 
AGGCTGGGGG TGCGGTTTGG GGAGTGGAGT GGTGGGCCTG GGCTCTCCCT GTGCCCTCCG 
GGGGGCATCC AAGAAGTGAC CATCAACCAG AATCTGCTGA CCCCACTGAA GATTGAGATC 
GATCCCCAGT TCCAGGTGGT GCGGACGCAG GAGACCCAGG AGATCAGAAC CCTCAACAAC 
CAGTTTGCTT CCTTCATTGA CAAGGTGCGG TTCCTGGAGC AGCAGAACAA GGTCCTGGAG 
ACGAAGTGGC ATCTGCTGCA GCAACAGGGG TTGAGTGGCA GCCAGCAGGG CCTGGAGCCT 
GTCTTTGAGG CCTGCCTGGA TCAGCTCAGG AAGCAGCTGG AGCAGCTCCA GGGAGAACGA 
GGGGCTCTGG ATGCTGAGTT GAAGGCCTGC CGGGACCAGG AGGAGGAGTA TAAGTCCAAG 
TATGAGGAGG AGGCCCACAG GCGTGCCACA CTTGAGAACG ACTTTGTGGT CCTCAAGAAG 
GATGTGGATG GGGTTTTCCT GAGCAAGATG GAGTTGGAGG GCAAGCTGGA GGCTCTGAGA 
GAGTACCTCT ACTTCTTGAA GCATCTGAAT GAAGAAGAGC TGGGCCAGCT CCAGACCCAG 
GCCAGCGACA CGTCTGTGGT GCTGTCCATG GACAACAACC GCTACCTGGA CTTCAGCAGC 
ATCATCACTG AGGTCCGCGC CCGGTACGAG GAGATCGCCC GGAGCAGCAA GGCTGAGGCT 
GAGGCCTTGT ACCAGACCAA GTACCAGGAA CTTCAGGTGT CTGCCCAGCT TCATGGGGAC 
AGGATGCAGG AAACGAAAGT CCAGATCTCT CAGCTACACC AAGAGATTCA GAGGCTGCAG 
AGTCAGACTG AGAACCTCAA GAAGCAGAAC GCCAGCCTGC AGGCCGCCAT CACTGATGCT 
GAGCAGCGTG GGGAGCTGGC CCTCAAGGAC GCTCAGGCCA AGGTGGACGA GCTGGAGGCT 
GCTCTGAGGA TGGCCAAGCA GAACCTGGCC CGGCTGCTGT GCGAGTACCA GGAGCTGACG 
AGCACGAAGC TTTCCCTGGA TGTGGAGATT GCCACTTACC GCAGGCTGCT GGAGGGCGAG 
GAGTGCAGGA TGTCTGGGGA GTGCACCAGC CAGGTCACTA TCTCCTCGGT GGGAGGCAGC 
GCTGTCATGT CTGGAGGAGT TGGTGGAGGC TTGGGGAGCA CTTGTGGACT CGGTAGTGGG 
AAAGGCAGCC CTGGGTCCTG CTGCACCAGC ATTGTGACTG GAGGCTCCAA CATCATTCTG 
GGCTCTGGGA AGGACCCTGT TTTGGATTCC TGCTCTGTGT CTGGCTCCAG CGCTGGCTCC 
AGCTGCCACA CCATCCTGAA GAAGACAGTT GAGTCGAGTC TGAAGACATC CATCACCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KRT78 ( NM_173352.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_775487.2 Homo sapiens keratin 78 (KRT78), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19538
Clone ID Related Accession (Same CDS sequence)	NM_173352.4 , NM_173352.3
Accession Version	NM_173352.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	keratin, type II cytoskeletal 78 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290901.1, BX647095.1 and AC107016.20. This sequence is a reference standard in the RefSeqGene project. On Jul 15, 2014 this sequence version replaced NM_173352.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK096419.1, ERR279862.1360.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2156670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTCT CCCCATGCCG GGCCCAGAGG GGCTTCAGCG CTCGCTCAGC CTGTTCTGCT 
CGCTCAAGGG GCCGCAGCAG GGGAGGCTTC AGCAGCAGGG GCGGCTTCAG CAGCAGGAGC 
CTTAATTCCT TTGGGGGGTG CCTGGAAGGC TCTCGTGGGA GTACCTGGGG GTCAGGGGGT 
AGGCTGGGGG TGCGGTTTGG GGAGTGGAGT GGTGGGCCTG GGCTCTCCCT GTGCCCTCCG 
GGGGGCATCC AAGAAGTGAC CATCAACCAG AATCTGCTGA CCCCACTGAA GATTGAGATC 
GATCCCCAGT TCCAGGTGGT GCGGACGCAG GAGACCCAGG AGATCAGAAC CCTCAACAAC 
CAGTTTGCTT CCTTCATTGA CAAGGTGCGG TTCCTGGAGC AGCAGAACAA GGTCCTGGAG 
ACGAAGTGGC ATCTGCTGCA GCAACAGGGG TTGAGTGGCA GCCAGCAGGG CCTGGAGCCT 
GTCTTTGAGG CCTGCCTGGA TCAGCTCAGG AAGCAGCTGG AGCAGCTCCA GGGAGAACGA 
GGGGCTCTGG ATGCTGAGTT GAAGGCCTGC CGGGACCAGG AGGAGGAGTA TAAGTCCAAG 
TATGAGGAGG AGGCCCACAG GCGTGCCACA CTTGAGAACG ACTTTGTGGT CCTCAAGAAG 
GATGTGGATG GGGTTTTCCT GAGCAAGATG GAGTTGGAGG GCAAGCTGGA GGCTCTGAGA 
GAGTACCTCT ACTTCTTGAA GCATCTGAAT GAAGAAGAGC TGGGCCAGCT CCAGACCCAG 
GCCAGCGACA CGTCTGTGGT GCTGTCCATG GACAACAACC GCTACCTGGA CTTCAGCAGC 
ATCATCACTG AGGTCCGCGC CCGGTACGAG GAGATCGCCC GGAGCAGCAA GGCTGAGGCT 
GAGGCCTTGT ACCAGACCAA GTACCAGGAA CTTCAGGTGT CTGCCCAGCT TCATGGGGAC 
AGGATGCAGG AAACGAAAGT CCAGATCTCT CAGCTACACC AAGAGATTCA GAGGCTGCAG 
AGTCAGACTG AGAACCTCAA GAAGCAGAAC GCCAGCCTGC AGGCCGCCAT CACTGATGCT 
GAGCAGCGTG GGGAGCTGGC CCTCAAGGAC GCTCAGGCCA AGGTGGACGA GCTGGAGGCT 
GCTCTGAGGA TGGCCAAGCA GAACCTGGCC CGGCTGCTGT GCGAGTACCA GGAGCTGACG 
AGCACGAAGC TTTCCCTGGA TGTGGAGATT GCCACTTACC GCAGGCTGCT GGAGGGCGAG 
GAGTGCAGGA TGTCTGGGGA GTGCACCAGC CAGGTCACTA TCTCCTCGGT GGGAGGCAGC 
GCTGTCATGT CTGGAGGAGT TGGTGGAGGC TTGGGGAGCA CTTGTGGACT CGGTAGTGGG 
AAAGGCAGCC CTGGGTCCTG CTGCACCAGC ATTGTGACTG GAGGCTCCAA CATCATTCTG 
GGCTCTGGGA AGGACCCTGT TTTGGATTCC TGCTCTGTGT CTGGCTCCAG CGCTGGCTCC 
AGCTGCCACA CCATCCTGAA GAAGACAGTT GAGTCGAGTC TGAAGACATC CATCACCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775487.2
CDS	65..1627
Translation	MSLSPCRAQRGFSARSACSARSRGRSRGGFSSRGGFSSRSLNSFGGCLEGSRGSTWGSGGRLGVRFGEWSGGPGLSLCPPGGIQEVTINQNLLTPLKIEIDPQFQVVRTQETQEIRTLNNQFASFIDKVRFLEQQNKVLETKWHLLQQQGLSGSQQGLEPVFEACLDQLRKQLEQLQGERGALDAELKACRDQEEEYKSKYEEEAHRRATLENDFVVLKKDVDGVFLSKMELEGKLEALREYLYFLKHLNEEELGQLQTQASDTSVVLSMDNNRYLDFSSIITEVRARYEEIARSSKAEAEALYQTKYQELQVSAQLHGDRMQETKVQISQLHQEIQRLQSQTENLKKQNASLQAAITDAEQRGELALKDAQAKVDELEAALRMAKQNLARLLCEYQELTSTKLSLDVEIATYRRLLEGEECRMSGECTSQVTISSVGGSAVMSGGVGGGLGSTCGLGSGKGSPGSCCTSIVTGGSNIILGSGKDPVLDSCSVSGSSAGSSCHTILKKTVESSLKTSITY

Target ORF information:

RefSeq Version	NM_173352.3
Organism	Homo sapiens(human)
Definition	Homo sapiens keratin 78 (KRT78), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173352.3

ORF Insert Sequence:

ATGTCTCTCT CCCCATGCCG GGCCCAGAGG GGCTTCAGCG CTCGCTCAGC CTGTTCTGCT 
CGCTCAAGGG GCCGCAGCAG GGGAGGCTTC AGCAGCAGGG GCGGCTTCAG CAGCAGGAGC 
CTTAATTCCT TTGGGGGGTG CCTGGAAGGC TCTCGTGGGA GTACCTGGGG GTCAGGGGGT 
AGGCTGGGGG TGCGGTTTGG GGAGTGGAGT GGTGGGCCTG GGCTCTCCCT GTGCCCTCCG 
GGGGGCATCC AAGAAGTGAC CATCAACCAG AATCTGCTGA CCCCACTGAA GATTGAGATC 
GATCCCCAGT TCCAGGTGGT GCGGACGCAG GAGACCCAGG AGATCAGAAC CCTCAACAAC 
CAGTTTGCTT CCTTCATTGA CAAGGTGCGG TTCCTGGAGC AGCAGAACAA GGTCCTGGAG 
ACGAAGTGGC ATCTGCTGCA GCAACAGGGG TTGAGTGGCA GCCAGCAGGG CCTGGAGCCT 
GTCTTTGAGG CCTGCCTGGA TCAGCTCAGG AAGCAGCTGG AGCAGCTCCA GGGAGAACGA 
GGGGCTCTGG ATGCTGAGTT GAAGGCCTGC CGGGACCAGG AGGAGGAGTA TAAGTCCAAG 
TATGAGGAGG AGGCCCACAG GCGTGCCACA CTTGAGAACG ACTTTGTGGT CCTCAAGAAG 
GATGTGGATG GGGTTTTCCT GAGCAAGATG GAGTTGGAGG GCAAGCTGGA GGCTCTGAGA 
GAGTACCTCT ACTTCTTGAA GCATCTGAAT GAAGAAGAGC TGGGCCAGCT CCAGACCCAG 
GCCAGCGACA CGTCTGTGGT GCTGTCCATG GACAACAACC GCTACCTGGA CTTCAGCAGC 
ATCATCACTG AGGTCCGCGC CCGGTACGAG GAGATCGCCC GGAGCAGCAA GGCTGAGGCT 
GAGGCCTTGT ACCAGACCAA GTACCAGGAA CTTCAGGTGT CTGCCCAGCT TCATGGGGAC 
AGGATGCAGG AAACGAAAGT CCAGATCTCT CAGCTACACC AAGAGATTCA GAGGCTGCAG 
AGTCAGACTG AGAACCTCAA GAAGCAGAAC GCCAGCCTGC AGGCCGCCAT CACTGATGCT 
GAGCAGCGTG GGGAGCTGGC CCTCAAGGAC GCTCAGGCCA AGGTGGACGA GCTGGAGGCT 
GCTCTGAGGA TGGCCAAGCA GAACCTGGCC CGGCTGCTGT GCGAGTACCA GGAGCTGACG 
AGCACGAAGC TTTCCCTGGA TGTGGAGATT GCCACTTACC GCAGGCTGCT GGAGGGCGAG 
GAGTGCAGGA TGTCTGGGGA GTGCACCAGC CAGGTCACTA TCTCCTCGGT GGGAGGCAGC 
GCTGTCATGT CTGGAGGAGT TGGTGGAGGC TTGGGGAGCA CTTGTGGACT CGGTAGTGGG 
AAAGGCAGCC CTGGGTCCTG CTGCACCAGC ATTGTGACTG GAGGCTCCAA CATCATTCTG 
GGCTCTGGGA AGGACCCTGT TTTGGATTCC TGCTCTGTGT CTGGCTCCAG CGCTGGCTCC 
AGCTGCCACA CCATCCTGAA GAAGACAGTT GAGTCGAGTC TGAAGACATC CATCACCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KRT78 ( NM_001300814.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287743.1 Homo sapiens keratin 78 (KRT78), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu46368
Clone ID Related Accession (Same CDS sequence)	NM_001300814.1
Accession Version	NM_001300814.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	keratin, type II cytoskeletal 78 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX647095.1 and AC107016.20. On Jul 15, 2014 this sequence version replaced XM_005268695.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 1. The encoded isoform (2) has a distinct N-terminus, and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BX647095.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2156670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGGTC ATGAGGCTTC CCCTGCCCAG GTTGGGCAGG GGGACAGGGG AAAGGTGCGG 
TTCCTGGAGC AGCAGAACAA GGTCCTGGAG ACGAAGTGGC ATCTGCTGCA GCAACAGGGG 
TTGAGTGGCA GCCAGCAGGG CCTGGAGCCT GTCTTTGAGG CCTGCCTGGA TCAGCTCAGG 
AAGCAGCTGG AGCAGCTCCA GGGAGAACGA GGGGCTCTGG ATGCTGAGTT GAAGGCCTGC 
CGGGACCAGG AGGAGGAGTA TAAGTCCAAG TATGAGGAGG AGGCCCACAG GCGTGCCACA 
CTTGAGAACG ACTTTGTGGT CCTCAAGAAG GATGTGGATG GGGTTTTCCT GAGCAAGATG 
GAGTTGGAGG GCAAGCTGGA GGCTCTGAGA GAGTACCTCT ACTTCTTGAA GCATCTGAAT 
GAAGAAGAGC TGGGCCAGCT CCAGACCCAG GCCAGCGACA CGTCTGTGGT GCTGTCCATG 
GACAACAACC GCTACCTGGA CTTCAGCAGC ATCATCACTG AGGTCCGCGC CCGGTACGAG 
GAGATCGCCC GGAGCAGCAA GGCTGAGGCT GAGGCCTTGT ACCAGACCAA GTACCAGGAA 
CTTCAGGTGT CTGCCCAGCT TCATGGGGAC AGGATGCAGG AAACGAAAGT CCAGATCTCT 
CAGCTACACC AAGAGATTCA GAGGCTGCAG AGTCAGACTG AGAACCTCAA GAAGCAGAAC 
GCCAGCCTGC AGGCCGCCAT CACTGATGCT GAGCAGCGTG GGGAGCTGGC CCTCAAGGAC 
GCTCAGGCCA AGGTGGACGA GCTGGAGGCT GCTCTGAGGA TGGCCAAGCA GAACCTGGCC 
CGGCTGCTGT GCGAGTACCA GGAGCTGACG AGCACGAAGC TTTCCCTGGA TGTGGAGATT 
GCCACTTACC GCAGGCTGCT GGAGGGCGAG GAGTGCAGGA TGTCTGGGGA GTGCACCAGC 
CAGGTCACTA TCTCCTCGGT GGGAGGCAGC GCTGTCATGT CTGGAGGAGT TGGTGGAGGC 
TTGGGGAGCA CTTGTGGACT CGGTAGTGGG AAAGGCAGCC CTGGGTCCTG CTGCACCAGC 
ATTGTGACTG GAGGCTCCAA CATCATTCTG GGCTCTGGGA AGGACCCTGT TTTGGATTCC 
TGCTCTGTGT CTGGCTCCAG CGCTGGCTCC AGCTGCCACA CCATCCTGAA GAAGACAGTT 
GAGTCGAGTC TGAAGACATC CATCACCTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287743.1
CDS	13..1245
Translation	MEGHEASPAQVGQGDRGKVRFLEQQNKVLETKWHLLQQQGLSGSQQGLEPVFEACLDQLRKQLEQLQGERGALDAELKACRDQEEEYKSKYEEEAHRRATLENDFVVLKKDVDGVFLSKMELEGKLEALREYLYFLKHLNEEELGQLQTQASDTSVVLSMDNNRYLDFSSIITEVRARYEEIARSSKAEAEALYQTKYQELQVSAQLHGDRMQETKVQISQLHQEIQRLQSQTENLKKQNASLQAAITDAEQRGELALKDAQAKVDELEAALRMAKQNLARLLCEYQELTSTKLSLDVEIATYRRLLEGEECRMSGECTSQVTISSVGGSAVMSGGVGGGLGSTCGLGSGKGSPGSCCTSIVTGGSNIILGSGKDPVLDSCSVSGSSAGSSCHTILKKTVESSLKTSITY

Target ORF information:

RefSeq Version	NM_001300814.1
Organism	Homo sapiens(human)
Definition	Homo sapiens keratin 78 (KRT78), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300814.1

ORF Insert Sequence:

ATGGAAGGTC ATGAGGCTTC CCCTGCCCAG GTTGGGCAGG GGGACAGGGG AAAGGTGCGG 
TTCCTGGAGC AGCAGAACAA GGTCCTGGAG ACGAAGTGGC ATCTGCTGCA GCAACAGGGG 
TTGAGTGGCA GCCAGCAGGG CCTGGAGCCT GTCTTTGAGG CCTGCCTGGA TCAGCTCAGG 
AAGCAGCTGG AGCAGCTCCA GGGAGAACGA GGGGCTCTGG ATGCTGAGTT GAAGGCCTGC 
CGGGACCAGG AGGAGGAGTA TAAGTCCAAG TATGAGGAGG AGGCCCACAG GCGTGCCACA 
CTTGAGAACG ACTTTGTGGT CCTCAAGAAG GATGTGGATG GGGTTTTCCT GAGCAAGATG 
GAGTTGGAGG GCAAGCTGGA GGCTCTGAGA GAGTACCTCT ACTTCTTGAA GCATCTGAAT 
GAAGAAGAGC TGGGCCAGCT CCAGACCCAG GCCAGCGACA CGTCTGTGGT GCTGTCCATG 
GACAACAACC GCTACCTGGA CTTCAGCAGC ATCATCACTG AGGTCCGCGC CCGGTACGAG 
GAGATCGCCC GGAGCAGCAA GGCTGAGGCT GAGGCCTTGT ACCAGACCAA GTACCAGGAA 
CTTCAGGTGT CTGCCCAGCT TCATGGGGAC AGGATGCAGG AAACGAAAGT CCAGATCTCT 
CAGCTACACC AAGAGATTCA GAGGCTGCAG AGTCAGACTG AGAACCTCAA GAAGCAGAAC 
GCCAGCCTGC AGGCCGCCAT CACTGATGCT GAGCAGCGTG GGGAGCTGGC CCTCAAGGAC 
GCTCAGGCCA AGGTGGACGA GCTGGAGGCT GCTCTGAGGA TGGCCAAGCA GAACCTGGCC 
CGGCTGCTGT GCGAGTACCA GGAGCTGACG AGCACGAAGC TTTCCCTGGA TGTGGAGATT 
GCCACTTACC GCAGGCTGCT GGAGGGCGAG GAGTGCAGGA TGTCTGGGGA GTGCACCAGC 
CAGGTCACTA TCTCCTCGGT GGGAGGCAGC GCTGTCATGT CTGGAGGAGT TGGTGGAGGC 
TTGGGGAGCA CTTGTGGACT CGGTAGTGGG AAAGGCAGCC CTGGGTCCTG CTGCACCAGC 
ATTGTGACTG GAGGCTCCAA CATCATTCTG GGCTCTGGGA AGGACCCTGT TTTGGATTCC 
TGCTCTGTGT CTGGCTCCAG CGCTGGCTCC AGCTGCCACA CCATCCTGAA GAAGACAGTT 
GAGTCGAGTC TGAAGACATC CATCACCTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KRT78 ( XM_011538010.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536312.1 Homo sapiens keratin 78 (KRT78), transcript variant X1, mRNA.

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CloneID	OHu77478
Clone ID Related Accession (Same CDS sequence)	XM_011538010.1
Accession Version	XM_011538010.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	keratin, type II cytoskeletal 78 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTCT CCCCATGCCG GGCCCAGAGG GGCTTCAGCG CTCGCTCAGC CTGTTCTGCT 
CGCTCAAGGG GCCGCAGCAG GGGAGGCTTC AGCAGCAGGG GCGGCTTCAG CAGCAGGAGC 
CTTAATTCCT TTGGGGGGTG CCTGGAAGGC TCTCGTGGGA GTACCTGGGG GTCAGGGGGT 
AGGCTGGGGG TGCGGTTTGG GGAGTGGAGT GGTGGGCCTG GGCTCTCCCT GTGCCCTCCG 
GGGGGCATCC AAGAAGTGAC CATCAACCAG AATCTGCTGA CCCCACTGAA GATTGAGATC 
GATCCCCAGT TCCAGGTGGT GCGGACGCAG GAGACCCAGG AGATCAGAAC CCTCAACAAC 
CAGTTTGCTT CCTTCATTGA CAAGGTGCGG TTCCTGGAGC AGCAGAACAA GGTCCTGGAG 
ACGAAGTGGC ATCTGCTGCA GCAACAGGGG TTGAGTGGCA GCCAGCAGGG CCTGGAGCCT 
GTCTTTGAGG CCTGCCTGGA TCAGCTCAGG AAGCAGCTGG AGCAGCTCCA GGGAGAACGA 
GGGGCTCTGG ATGCTGAGTT GAAGGCCTGC CGGGACCAGG AGGAGGAGTA TAAGTCCAAG 
TATGAGGAGG AGGCCCACAG GCGTGCCACA CTTGAGAACG ACTTTGTGGT CCTCAAGAAG 
GAGCTGGGCC AGCTCCAGAC CCAGGCCAGC GACACGTCTG TGGTGCTGTC CATGGACAAC 
AACCGCTACC TGGACTTCAG CAGCATCATC ACTGAGGTCC GCGCCCGGTA CGAGGAGATC 
GCCCGGAGCA GCAAGGCTGA GGCTGAGGCC TTGTACCAGA CCAAGTACCA GGAACTTCAG 
GTGTCTGCCC AGCTTCATGG GGACAGGATG CAGGAAACGA AAGTCCAGAT CTCTCAGCTA 
CACCAAGAGA TTCAGAGGCT GCAGAGTCAG ACTGAGAACC TCAAGAAGCA GAACGCCAGC 
CTGCAGGCCG CCATCACTGA TGCTGAGCAG CGTGGGGAGC TGGCCCTCAA GGACGCTCAG 
GCCAAGGTGG ACGAGCTGGA GGCTGCTCTG AGGATGGCCA AGCAGAACCT GGCCCGGCTG 
CTGTGCGAGT ACCAGGAGCT GACGAGCACG AAGCTTTCCC TGGATGTGGA GATTGCCACT 
TACCGCAGGC TGCTGGAGGG CGAGGAGTGC AGGATGTCTG GGGAGTGCAC CAGCCAGGTC 
ACTATCTCCT CGGTGGGAGG CAGCGCTGTC ATGTCTGGAG GAGTTGGTGG AGGCTTGGGG 
AGCACTTGTG GACTCGGTAG TGGGAAAGGC AGCCCTGGGT CCTGCTGCAC CAGCATTGTG 
ACTGGAGGCT CCAACATCAT TCTGGGCTCT GGGAAGGACC CTGTTTTGGA TTCCTGCTCT 
GTGTCTGGCT CCAGCGCTGG CTCCAGCTGC CACACCATCC TGAAGAAGAC AGTTGAGTCG 
AGTCTGAAGA CATCCATCAC CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536312.1
CDS	65..1531
Translation	MSLSPCRAQRGFSARSACSARSRGRSRGGFSSRGGFSSRSLNSFGGCLEGSRGSTWGSGGRLGVRFGEWSGGPGLSLCPPGGIQEVTINQNLLTPLKIEIDPQFQVVRTQETQEIRTLNNQFASFIDKVRFLEQQNKVLETKWHLLQQQGLSGSQQGLEPVFEACLDQLRKQLEQLQGERGALDAELKACRDQEEEYKSKYEEEAHRRATLENDFVVLKKELGQLQTQASDTSVVLSMDNNRYLDFSSIITEVRARYEEIARSSKAEAEALYQTKYQELQVSAQLHGDRMQETKVQISQLHQEIQRLQSQTENLKKQNASLQAAITDAEQRGELALKDAQAKVDELEAALRMAKQNLARLLCEYQELTSTKLSLDVEIATYRRLLEGEECRMSGECTSQVTISSVGGSAVMSGGVGGGLGSTCGLGSGKGSPGSCCTSIVTGGSNIILGSGKDPVLDSCSVSGSSAGSSCHTILKKTVESSLKTSITY

Target ORF information:

RefSeq Version	XM_011538010.1
Organism	Homo sapiens(human)
Definition	Homo sapiens keratin 78 (KRT78), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538010.1

ORF Insert Sequence:

ATGTCTCTCT CCCCATGCCG GGCCCAGAGG GGCTTCAGCG CTCGCTCAGC CTGTTCTGCT 
CGCTCAAGGG GCCGCAGCAG GGGAGGCTTC AGCAGCAGGG GCGGCTTCAG CAGCAGGAGC 
CTTAATTCCT TTGGGGGGTG CCTGGAAGGC TCTCGTGGGA GTACCTGGGG GTCAGGGGGT 
AGGCTGGGGG TGCGGTTTGG GGAGTGGAGT GGTGGGCCTG GGCTCTCCCT GTGCCCTCCG 
GGGGGCATCC AAGAAGTGAC CATCAACCAG AATCTGCTGA CCCCACTGAA GATTGAGATC 
GATCCCCAGT TCCAGGTGGT GCGGACGCAG GAGACCCAGG AGATCAGAAC CCTCAACAAC 
CAGTTTGCTT CCTTCATTGA CAAGGTGCGG TTCCTGGAGC AGCAGAACAA GGTCCTGGAG 
ACGAAGTGGC ATCTGCTGCA GCAACAGGGG TTGAGTGGCA GCCAGCAGGG CCTGGAGCCT 
GTCTTTGAGG CCTGCCTGGA TCAGCTCAGG AAGCAGCTGG AGCAGCTCCA GGGAGAACGA 
GGGGCTCTGG ATGCTGAGTT GAAGGCCTGC CGGGACCAGG AGGAGGAGTA TAAGTCCAAG 
TATGAGGAGG AGGCCCACAG GCGTGCCACA CTTGAGAACG ACTTTGTGGT CCTCAAGAAG 
GAGCTGGGCC AGCTCCAGAC CCAGGCCAGC GACACGTCTG TGGTGCTGTC CATGGACAAC 
AACCGCTACC TGGACTTCAG CAGCATCATC ACTGAGGTCC GCGCCCGGTA CGAGGAGATC 
GCCCGGAGCA GCAAGGCTGA GGCTGAGGCC TTGTACCAGA CCAAGTACCA GGAACTTCAG 
GTGTCTGCCC AGCTTCATGG GGACAGGATG CAGGAAACGA AAGTCCAGAT CTCTCAGCTA 
CACCAAGAGA TTCAGAGGCT GCAGAGTCAG ACTGAGAACC TCAAGAAGCA GAACGCCAGC 
CTGCAGGCCG CCATCACTGA TGCTGAGCAG CGTGGGGAGC TGGCCCTCAA GGACGCTCAG 
GCCAAGGTGG ACGAGCTGGA GGCTGCTCTG AGGATGGCCA AGCAGAACCT GGCCCGGCTG 
CTGTGCGAGT ACCAGGAGCT GACGAGCACG AAGCTTTCCC TGGATGTGGA GATTGCCACT 
TACCGCAGGC TGCTGGAGGG CGAGGAGTGC AGGATGTCTG GGGAGTGCAC CAGCCAGGTC 
ACTATCTCCT CGGTGGGAGG CAGCGCTGTC ATGTCTGGAG GAGTTGGTGG AGGCTTGGGG 
AGCACTTGTG GACTCGGTAG TGGGAAAGGC AGCCCTGGGT CCTGCTGCAC CAGCATTGTG 
ACTGGAGGCT CCAACATCAT TCTGGGCTCT GGGAAGGACC CTGTTTTGGA TTCCTGCTCT 
GTGTCTGGCT CCAGCGCTGG CTCCAGCTGC CACACCATCC TGAAGAAGAC AGTTGAGTCG 
AGTCTGAAGA CATCCATCAC CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Localisation of keratin K78 in the basal layer and first suprabasal layers of stratified epithelia completes expression catalogue of type II keratins and provides new insights into sequential keratin expression.
Cell and tissue research363(3)735-50(2016 Mar)
Langbein L,Eckhart L,Fischer H,Rogers MA,Praetzel-Wunder S,Parry DA,Kittstein W,Schweizer J

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
Oncology reports28(2)429-38(2012 Aug)
B?hm D,Keller K,Pieter J,Boehm N,Wolters D,Siggelkow W,Lebrecht A,Schmidt M,K?lbl H,Pfeiffer N,Grus FH

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Journal of proteome research8(3)1304-14(2009 Mar)
Gonzalez-Begne M,Lu B,Han X,Hagen FK,Hand AR,Melvin JE,Yates JR

New consensus nomenclature for mammalian keratins.
The Journal of cell biology174(2)169-74(2006 Jul)
Schweizer J,Bowden PE,Coulombe PA,Langbein L,Lane EB,Magin TM,Maltais L,Omary MB,Parry DA,Rogers MA,Wright MW

Characterization of new members of the human type II keratin gene family and a general evaluation of the keratin gene domain on chromosome 12q13.13.
The Journal of investigative dermatology124(3)536-44(2005 Mar)
Rogers MA,Edler L,Winter H,Langbein L,Beckmann I,Schweizer J

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KRT78 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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