HINT3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HINT3
Entrez Gene ID	135114
Full Name	histidine triad nucleotide binding protein 3
Synonyms	HINT4
General protein information	Preferred Names histidine triad nucleotide binding protein 3 Names histidine triad nucleotide-binding protein 3 HINT-3 HIT-like protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q22.32
Summary	Histidine triad proteins, such as HINT3, are nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides (Brenner, 2002 [PubMed 12119013]).[supplied by OMIM, Mar 2008].
Disorder MIM:	609998

mRNA and Protein(s)

mRNA	Protein	Name
NM_138571.5	NP_612638.3	histidine triad nucleotide-binding protein 3
NM_138571.4	NP_612638.3	histidine triad nucleotide-binding protein 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HINT3	XP_001165850.1
Canis lupus familiaris (dog)	HINT3	XP_533489.2
	HINT3	XP_001107408.1
Mus musculus (house mouse)	Hint3	NP_080074.1
Gallus gallus (chicken)	HINT3	XP_001232866.1
Danio rerio (zebrafish)	hint3	XP_001334761.2
Xenopus tropicalis (tropical clawed frog)	hint3	NP_001039146.1
Drosophila melanogaster (fruit fly)	CG15362	NP_608634.1
Homo sapiens (human)	HINT3	NP_612638.3
Bos taurus (cattle)	HINT3	NP_776766.1
Rattus norvegicus (Norway rat)	Hint3	NP_001094295.1
Drosophila melanogaster (fruit fly)	CG34015	NP_001033848.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007577	XP_308297.3

Gene Ontology
Bibliography

Related articles in PubMed

Evidence that human histidine triad nucleotide binding protein 3 (Hint3) is a distinct branch of the histidine triad (HIT) superfamily.
Chou TF, Cheng J, Tikh IB, Wagner CR
Journal of molecular biology373(4)978-89(2007 Nov)

Hint, Fhit, and GalT: function, structure, evolution, and mechanism of three branches of the histidine triad superfamily of nucleotide hydrolases and transferases.
Brenner C
Biochemistry41(29)9003-14(2002 Jul)

Adenosine monophosphoramidase activity of Hint and Hnt1 supports function of Kin28, Ccl1, and Tfb3.
Bieganowski P, Garrison PN, Hodawadekar SC, Faye G, Barnes LD, Brenner C
The Journal of biological chemistry277(13)10852-60(2002 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that significant differences in phosphoramidase activity, oligomeric state, and cellular localization suggest that Hint3s should be placed in a distinct branch of the histidine triad superfamily.
Title: Evidence that human histidine triad nucleotide binding protein 3 (Hint3) is a distinct branch of the histidine triad (HIT) superfamily.

The following HINT3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HINT3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24324	NM_138571.5 Latest version!	Homo sapiens histidine triad nucleotide binding protein 3 (HINT3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu24324	NM_138571.4	Homo sapiens histidine triad nucleotide binding protein 3 (HINT3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HINT3 ( NM_138571.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_612638.3 Homo sapiens histidine triad nucleotide binding protein 3 (HINT3), mRNA.

Price & Availability↑

CloneID	OHu24324
Clone ID Related Accession (Same CDS sequence)	NM_138571.5 , NM_138571.4
Accession Version	NM_138571.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	histidine triad nucleotide-binding protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA393891.1, AY035387.1, BC015732.1, DA997963.1, CX755653.1, AL035689.26 and BX647228.1. On Nov 22, 2018 this sequence version replaced NM_138571.4. ##Evidence-Data-START## Transcript exon combination :: BX647228.1, SRR1660807.176584.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000229633.7/ ENSP00000229633.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGAGG AACAGGTGAA CCGCAGCGCC GGCCTGGCCC CCGACTGTGA GGCCTCGGCG 
ACTGCAGAAA CTACGGTTTC CTCAGTGGGG ACCTGTGAAG CCGCTGGCAA GTCACCAGAG 
CCCAAGGACT ACGACAGCAC CTGCGTGTTC TGCCGGATCG CGGGGCGGCA GGACCCGGGC 
ACCGAACTCC TGCACTGCGA GAATGAGGAC CTAATTTGCT TCAAAGATAT CAAACCAGCA 
GCAACTCATC ATTATCTTGT GGTGCCAAAG AAGCATATTG GAAACTGCAG AACTCTAAGG 
AAAGATCAAG TAGAACTGGT TGAGAACATG GTAACTGTTG GAAAAACCAT TCTTGAAAGA 
AATAATTTCA CTGACTTCAC GAATGTGAGG ATGGGTTTTC ATATGCCACC ATTCTGTTCC 
ATTTCCCACT TGCACCTTCA TGTTCTGGCA CCAGTGGATC AGCTTGGCTT CTTATCCAAG 
TTGGTTTATA GAGTCAATTC CTATTGGTTT ATCACAGCTG ATCACTTGAT TGAAAAACTA 
AGAACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612638.3
CDS	209..757
Translation	MAEEQVNRSAGLAPDCEASATAETTVSSVGTCEAAGKSPEPKDYDSTCVFCRIAGRQDPGTELLHCENEDLICFKDIKPAATHHYLVVPKKHIGNCRTLRKDQVELVENMVTVGKTILERNNFTDFTNVRMGFHMPPFCSISHLHLHVLAPVDQLGFLSKLVYRVNSYWFITADHLIEKLRT

Target ORF information:

RefSeq Version	NM_138571.5
Organism	Homo sapiens(human)
Definition	Homo sapiens histidine triad nucleotide binding protein 3 (HINT3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138571.5

ORF Insert Sequence:

ATGGCGGAGG AACAGGTGAA CCGCAGCGCC GGCCTGGCCC CCGACTGTGA GGCCTCGGCG 
ACTGCAGAAA CTACGGTTTC CTCAGTGGGG ACCTGTGAAG CCGCTGGCAA GTCACCAGAG 
CCCAAGGACT ACGACAGCAC CTGCGTGTTC TGCCGGATCG CGGGGCGGCA GGACCCGGGC 
ACCGAACTCC TGCACTGCGA GAATGAGGAC CTAATTTGCT TCAAAGATAT CAAACCAGCA 
GCAACTCATC ATTATCTTGT GGTGCCAAAG AAGCATATTG GAAACTGCAG AACTCTAAGG 
AAAGATCAAG TAGAACTGGT TGAGAACATG GTAACTGTTG GAAAAACCAT TCTTGAAAGA 
AATAATTTCA CTGACTTCAC GAATGTGAGG ATGGGTTTTC ATATGCCACC ATTCTGTTCC 
ATTTCCCACT TGCACCTTCA TGTTCTGGCA CCAGTGGATC AGCTTGGCTT CTTATCCAAG 
TTGGTTTATA GAGTCAATTC CTATTGGTTT ATCACAGCTG ATCACTTGAT TGAAAAACTA 
AGAACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HINT3 ( NM_138571.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_612638.3 Homo sapiens histidine triad nucleotide binding protein 3 (HINT3), mRNA.

Price & Availability↑

CloneID	OHu24324
Clone ID Related Accession (Same CDS sequence)	NM_138571.5 , NM_138571.4
Accession Version	NM_138571.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	histidine triad nucleotide-binding protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA393891.1, AY035387.1, BC015732.1, DA997963.1, CX755653.1, AL035689.26 and BX647228.1. On May 31, 2008 this sequence version replaced NM_138571.3. ##Evidence-Data-START## Transcript exon combination :: BX647228.1, SRR1660805.241430.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGG AACAGGTGAA CCGCAGCGCC GGCCTGGCCC CCGACTGTGA GGCCTCGGCG 
ACTGCAGAAA CTACGGTTTC CTCAGTGGGG ACCTGTGAAG CCGCTGGCAA GTCACCAGAG 
CCCAAGGACT ACGACAGCAC CTGCGTGTTC TGCCGGATCG CGGGGCGGCA GGACCCGGGC 
ACCGAACTCC TGCACTGCGA GAATGAGGAC CTAATTTGCT TCAAAGATAT CAAACCAGCA 
GCAACTCATC ATTATCTTGT GGTGCCAAAG AAGCATATTG GAAACTGCAG AACTCTAAGG 
AAAGATCAAG TAGAACTGGT TGAGAACATG GTAACTGTTG GAAAAACCAT TCTTGAAAGA 
AATAATTTCA CTGACTTCAC GAATGTGAGG ATGGGTTTTC ATATGCCACC ATTCTGTTCC 
ATTTCCCACT TGCACCTTCA TGTTCTGGCA CCAGTGGATC AGCTTGGCTT CTTATCCAAG 
TTGGTTTATA GAGTCAATTC CTATTGGTTT ATCACAGCTG ATCACTTGAT TGAAAAACTA 
AGAACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612638.3
CDS	264..812
Translation	MAEEQVNRSAGLAPDCEASATAETTVSSVGTCEAAGKSPEPKDYDSTCVFCRIAGRQDPGTELLHCENEDLICFKDIKPAATHHYLVVPKKHIGNCRTLRKDQVELVENMVTVGKTILERNNFTDFTNVRMGFHMPPFCSISHLHLHVLAPVDQLGFLSKLVYRVNSYWFITADHLIEKLRT

Target ORF information:

RefSeq Version	NM_138571.4
Organism	Homo sapiens(human)
Definition	Homo sapiens histidine triad nucleotide binding protein 3 (HINT3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138571.4

ORF Insert Sequence:

ATGGCGGAGG AACAGGTGAA CCGCAGCGCC GGCCTGGCCC CCGACTGTGA GGCCTCGGCG 
ACTGCAGAAA CTACGGTTTC CTCAGTGGGG ACCTGTGAAG CCGCTGGCAA GTCACCAGAG 
CCCAAGGACT ACGACAGCAC CTGCGTGTTC TGCCGGATCG CGGGGCGGCA GGACCCGGGC 
ACCGAACTCC TGCACTGCGA GAATGAGGAC CTAATTTGCT TCAAAGATAT CAAACCAGCA 
GCAACTCATC ATTATCTTGT GGTGCCAAAG AAGCATATTG GAAACTGCAG AACTCTAAGG 
AAAGATCAAG TAGAACTGGT TGAGAACATG GTAACTGTTG GAAAAACCAT TCTTGAAAGA 
AATAATTTCA CTGACTTCAC GAATGTGAGG ATGGGTTTTC ATATGCCACC ATTCTGTTCC 
ATTTCCCACT TGCACCTTCA TGTTCTGGCA CCAGTGGATC AGCTTGGCTT CTTATCCAAG 
TTGGTTTATA GAGTCAATTC CTATTGGTTT ATCACAGCTG ATCACTTGAT TGAAAAACTA 
AGAACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evidence that human histidine triad nucleotide binding protein 3 (Hint3) is a distinct branch of the histidine triad (HIT) superfamily.
Journal of molecular biology373(4)978-89(2007 Nov)
Chou TF,Cheng J,Tikh IB,Wagner CR

Hint, Fhit, and GalT: function, structure, evolution, and mechanism of three branches of the histidine triad superfamily of nucleotide hydrolases and transferases.
Biochemistry41(29)9003-14(2002 Jul)
Brenner C

Adenosine monophosphoramidase activity of Hint and Hnt1 supports function of Kin28, Ccl1, and Tfb3.
The Journal of biological chemistry277(13)10852-60(2002 Mar)
Bieganowski P,Garrison PN,Hodawadekar SC,Faye G,Barnes LD,Brenner C