COL8A2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL8A2
Entrez Gene ID	1296
Full Name	collagen type VIII alpha 2 chain
Synonyms	FECD,FECD1,PPCD,PPCD2
General protein information	Preferred Names collagen type VIII alpha 2 chain Names collagen alpha-2(VIII) chain collagen VIII, alpha-2 polypeptide collagen, type VIII, alpha 2 endothelial collagen
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p34.3
Summary	This gene encodes the alpha 2 chain of type VIII collagen. This protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014].
Disorder MIM:	120252
Disorder Html:	Corneal dystrophy, Fuchs endothelial, 1, 136800 (3); Corneal dystrophy, posterior polymorphous 2, 609140 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_005202.3	NP_005193.1	collagen alpha-2(VIII) chain isoform 1 precursor
NM_001294347.1	NP_001281276.1	collagen alpha-2(VIII) chain isoform 2
XM_005270477.3	XP_005270534.1	collagen alpha-2(VIII) chain isoform X1
NM_001294347.2	NP_001281276.1	collagen alpha-2(VIII) chain isoform 2
NM_005202.4	NP_005193.1	collagen alpha-2(VIII) chain isoform 1 precursor

Pathways from BioSystems

Homologies

	COL8A2	XP_001102808.2
Bos taurus (cattle)	COL8A2	NP_001192594.1
Danio rerio (zebrafish)	col8a2	NP_001076370.1
Pan troglodytes (chimpanzee)	COL8A2	XP_001158595.2
Mus musculus (house mouse)	Col8a2	NP_955767.1
Homo sapiens (human)	COL8A2	NP_005193.1
Canis lupus familiaris (dog)	COL8A2	XP_003639551.1
Rattus norvegicus (Norway rat)	Col8a2	XP_006238974.1
Gallus gallus (chicken)	COL8A2	XP_425780.3
Xenopus tropicalis (tropical clawed frog)	col8a2	XP_002936271.2

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Naba A, Pearce OMT, Del Rosario A, Ma D, Ding H, Rajeeve V, Cutillas PR, Balkwill FR, Hynes RO
Journal of proteome research16(8)3083-3091(2017 Aug)

Analysis of SLC4A11, ZEB1, LOXHD1, COL8A2 and TCF4 gene sequences in a multi-generational family with late-onset Fuchs corneal dystrophy.
Tang H, Zhang W, Yan XM, Wang LP, Dong H, Shou T, Lei H, Guo Q
International journal of molecular medicine37(6)1487-500(2016 Jun)

Distinct Clinical Phenotype of Corneal Dystrophy Predicts the p.(Leu450Trp) Substitution in COL8A2.
Eghrari AO, Riazuddin SA, Gottsch JD
Cornea35(5)587-91(2016 May)

Biosynthetic and functional defects in newly identified SLC4A11 mutants and absence of COL8A2 mutations in Fuchs endothelial corneal dystrophy.
Soumittra N, Loganathan SK, Madhavan D, Ramprasad VL, Arokiasamy T, Sumathi S, Karthiyayini T, Rachapalli SR, Kumaramanickavel G, Casey JR, Rajagopal R
Journal of human genetics59(8)444-53(2014 Aug)

Extracellular matrix signatures of human primary metastatic colon cancers and their metastases to liver.
Naba A, Clauser KR, Whittaker CA, Carr SA, Tanabe KK, Hynes RO
BMC cancer14518(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Analysis of SLC4A11, ZEB1, LOXHD1, COL8A2 and TCF4 gene sequences in a multi-generational family with late-onset Fuchs corneal dystrophy found no evidence for found polymorophisms causing the disease in this specific pedigree.
Title: Analysis of SLC4A11, ZEB1, LOXHD1, COL8A2 and TCF4 gene sequences in a multi-generational family with late-onset Fuchs corneal dystrophy.

Peripheral, anterior microcystic corneal edema represents a characteristic aspect of the phenotype associated with the p.(Leu450Trp) substitution in COL8A2, in at least 2 of 3 known affected families worldwide.
Title: Distinct Clinical Phenotype of Corneal Dystrophy Predicts the p.(Leu450Trp) Substitution in COL8A2.

No mutations were identified in COL8A2, in neither the late-onset cohort nor the early-onset family, suggesting genetic heterogeneity in this Late-onset Fuchs endothelial corneal dystrophy (FECD) family.
Title: Biosynthetic and functional defects in newly identified SLC4A11 mutants and absence of COL8A2 mutations in Fuchs endothelial corneal dystrophy.

Esophageal transcript profiling identified a distinct subset of genes, including COL8A2, in patients with Eosionophilic esophagitis and inherited connective tissue disorders.
Title: High prevalence of eosinophilic esophagitis in patients with inherited connective tissue disorders.

Variation in the COL8A2, SLC4A11, and ZEB1 genes is present in only a small fraction of African American cases and as such does not appear to significantly contribute to the genetic risk of Fuchs endothelial corneal dystrophy.
Title: Genetic screen of African Americans with Fuchs endothelial corneal dystrophy.

The following COL8A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL8A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28708	NM_001294347.2 Latest version!	Homo sapiens collagen type VIII alpha 2 chain (COL8A2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu28719	NM_005202.4 Latest version!	Homo sapiens collagen type VIII alpha 2 chain (COL8A2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$496.30 $709.00
OHu33026	XM_005270477.3 Latest version!	Homo sapiens collagen type VIII alpha 2 chain (COL8A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OHu28708	NM_001294347.1	Homo sapiens collagen type VIII alpha 2 chain (COL8A2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu28719	NM_005202.3	Homo sapiens collagen type VIII alpha 2 chain (COL8A2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$496.30 $709.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL8A2 ( NM_001294347.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001281276.1 Homo sapiens collagen type VIII alpha 2 chain (COL8A2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28708
Clone ID Related Accession (Same CDS sequence)	NM_001294347.1 , NM_001294347.2
Accession Version	NM_001294347.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Homo sapiens(human)
Product	collagen alpha-2(VIII) chain isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY025990.1, AK074129.1, AL138787.11 and AI084614.1. On Jul 1, 2014 this sequence version replaced XM_005270479.2. Transcript Variant: This variant (2) uses an alternate splice site in the 5' region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY025990.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA2142348 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCTAC CGCTGCTGCC GATGGACCTG AAGGGAGAGC CCGGCCCCCC TGGGAAGCCC 
GGGCCTCGGG GTCCCCCTGG CCCCCCTGGC TTCCCAGGAA AACCAGGCAT GGGAAAGCCA 
GGACTCCATG GGCAGCCTGG CCCTGCTGGG CCCCCTGGCT TCTCCCGGAT GGGCAAGGCT 
GGTCCCCCAG GGCTCCCTGG CAAGGTCGGG CCACCAGGGC AGCCGGGGCT TCGGGGGGAG 
CCAGGAATAC GAGGGGACCA GGGCCTCCGG GGACCCCCAG GACCCCCTGG CCTCCCGGGC 
CCCTCAGGCA TTACTATCCC TGGAAAACCA GGTGCCCAAG GGGTGCCAGG GCCCCCAGGA 
TTCCAGGGGG AACCAGGGCC CCAGGGGGAG CCTGGGCCCC CAGGTGATCG AGGCCTCAAG 
GGGGATAATG GAGTGGGCCA GCCCGGGCTG CCTGGGGCCC CAGGGCAGGG GGGTGCCCCC 
GGCCCCCCCG GCCTCCCTGG TCCAGCTGGC TTAGGCAAAC CTGGTTTGGA TGGGCTTCCT 
GGGGCCCCAG GAGACAAGGG TGAGTCTGGG CCTCCTGGAG TTCCAGGCCC CAGGGGGGAG 
CCAGGAGCTG TGGGCCCAAA AGGACCTCCT GGAGTAGACG GTGTGGGAGT CCCAGGGGCA 
GCAGGGTTGC CAGGACCACA GGGCCCATCA GGGGCCAAAG GGGAGCCAGG GACCCGGGGC 
CCCCCTGGGC TGATAGGCCC CACTGGCTAT GGGATGCCAG GACTGCCAGG CCCCAAGGGG 
GACAGGGGCC CAGCTGGGGT CCCAGGACTC TTGGGGGACA GGGGTGAGCC AGGGGAGGAT 
GGGGAGCCAG GGGAGCAGGG CCCACAGGGT CTTGGGGGTC CCCCTGGACT TCCTGGGTCT 
GCAGGGCTTC CTGGCAGACG TGGGCCCCCT GGGCCTAAGG GTGAGGCAGG GCCTGGAGGA 
CCCCCAGGAG TGCCTGGCAT TCGAGGTGAC CAGGGGCCTA GTGGCCTGGC TGGGAAACCA 
GGGGTCCCAG GTGAGAGGGG ACTTCCTGGG GCCCATGGAC CCCCTGGACC AACTGGGCCC 
AAGGGTGAGC CGGGTTTCAC GGGTCGCCCT GGAGGACCAG GGGTGGCAGG AGCCCTGGGG 
CAGAAAGGTG ACTTGGGGCT CCCTGGGCAG CCTGGCCTGA GGGGTCCCTC AGGAATCCCA 
GGACTCCAGG GTCCAGCTGG CCCTATTGGG CCCCAAGGCC TGCCGGGCCT GAAGGGGGAA 
CCAGGCCTGC CAGGGCCCCC TGGAGAGGGG AGAGCAGGGG AACCTGGCAC GGCTGGGCCC 
ACGGGGCCCC CAGGGGTCCC TGGCTCCCCT GGAATCACGG GCCCTCCGGG GCCTCCCGGG 
CCCCCGGGAC CCCCTGGTGC CCCTGGGGCC TTCGATGAGA CTGGCATCGC AGGCTTGCAC 
CTGCCCAACG GCGGTGTGGA GGGTGCCGTG CTGGGCAAGG GGGGCAAGCC ACAGTTTGGG 
CTGGGCGAGC TGTCTGCCCA TGCCACACCG GCCTTCACTG CGGTGCTCAC CTCGCCCTTC 
CCCGCCTCGG GCATGCCCGT GAAATTTGAC CGGACTCTCT ACAATGGCCA CAGCGGCTAC 
AACCCAGCCA CTGGCATCTT CACCTGCCCT GTGGGCGGCG TCTACTACTT TGCTTACCAT 
GTGCACGTCA AGGGCACCAA CGTGTGGGTG GCCCTGTACA AGAACAACGT GCCGGCCACC 
TATACCTACG ATGAGTACAA GAAGGGCTAC CTGGACCAGG CATCTGGTGG GGCCGTGCTC 
CAGCTGCGGC CCAACGACCA GGTCTGGGTG CAGATGCCGT CGGACCAGGC CAACGGCCTC 
TACTCCACGG AGTACATCCA CTCCTCCTTT TCAGGATTCT TGCTCTGCCC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001281276.1
CDS	140..2056
Translation	MPLPLLPMDLKGEPGPPGKPGPRGPPGPPGFPGKPGMGKPGLHGQPGPAGPPGFSRMGKAGPPGLPGKVGPPGQPGLRGEPGIRGDQGLRGPPGPPGLPGPSGITIPGKPGAQGVPGPPGFQGEPGPQGEPGPPGDRGLKGDNGVGQPGLPGAPGQGGAPGPPGLPGPAGLGKPGLDGLPGAPGDKGESGPPGVPGPRGEPGAVGPKGPPGVDGVGVPGAAGLPGPQGPSGAKGEPGTRGPPGLIGPTGYGMPGLPGPKGDRGPAGVPGLLGDRGEPGEDGEPGEQGPQGLGGPPGLPGSAGLPGRRGPPGPKGEAGPGGPPGVPGIRGDQGPSGLAGKPGVPGERGLPGAHGPPGPTGPKGEPGFTGRPGGPGVAGALGQKGDLGLPGQPGLRGPSGIPGLQGPAGPIGPQGLPGLKGEPGLPGPPGEGRAGEPGTAGPTGPPGVPGSPGITGPPGPPGPPGPPGAPGAFDETGIAGLHLPNGGVEGAVLGKGGKPQFGLGELSAHATPAFTAVLTSPFPASGMPVKFDRTLYNGHSGYNPATGIFTCPVGGVYYFAYHVHVKGTNVWVALYKNNVPATYTYDEYKKGYLDQASGGAVLQLRPNDQVWVQMPSDQANGLYSTEYIHSSFSGFLLCPT

Target ORF information:

RefSeq Version	NM_001294347.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VIII alpha 2 chain (COL8A2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001294347.1

ORF Insert Sequence:

ATGCCTCTAC CGCTGCTGCC GATGGACCTG AAGGGAGAGC CCGGCCCCCC TGGGAAGCCC 
GGGCCTCGGG GTCCCCCTGG CCCCCCTGGC TTCCCAGGAA AACCAGGCAT GGGAAAGCCA 
GGACTCCATG GGCAGCCTGG CCCTGCTGGG CCCCCTGGCT TCTCCCGGAT GGGCAAGGCT 
GGTCCCCCAG GGCTCCCTGG CAAGGTCGGG CCACCAGGGC AGCCGGGGCT TCGGGGGGAG 
CCAGGAATAC GAGGGGACCA GGGCCTCCGG GGACCCCCAG GACCCCCTGG CCTCCCGGGC 
CCCTCAGGCA TTACTATCCC TGGAAAACCA GGTGCCCAAG GGGTGCCAGG GCCCCCAGGA 
TTCCAGGGGG AACCAGGGCC CCAGGGGGAG CCTGGGCCCC CAGGTGATCG AGGCCTCAAG 
GGGGATAATG GAGTGGGCCA GCCCGGGCTG CCTGGGGCCC CAGGGCAGGG GGGTGCCCCC 
GGCCCCCCCG GCCTCCCTGG TCCAGCTGGC TTAGGCAAAC CTGGTTTGGA TGGGCTTCCT 
GGGGCCCCAG GAGACAAGGG TGAGTCTGGG CCTCCTGGAG TTCCAGGCCC CAGGGGGGAG 
CCAGGAGCTG TGGGCCCAAA AGGACCTCCT GGAGTAGACG GTGTGGGAGT CCCAGGGGCA 
GCAGGGTTGC CAGGACCACA GGGCCCATCA GGGGCCAAAG GGGAGCCAGG GACCCGGGGC 
CCCCCTGGGC TGATAGGCCC CACTGGCTAT GGGATGCCAG GACTGCCAGG CCCCAAGGGG 
GACAGGGGCC CAGCTGGGGT CCCAGGACTC TTGGGGGACA GGGGTGAGCC AGGGGAGGAT 
GGGGAGCCAG GGGAGCAGGG CCCACAGGGT CTTGGGGGTC CCCCTGGACT TCCTGGGTCT 
GCAGGGCTTC CTGGCAGACG TGGGCCCCCT GGGCCTAAGG GTGAGGCAGG GCCTGGAGGA 
CCCCCAGGAG TGCCTGGCAT TCGAGGTGAC CAGGGGCCTA GTGGCCTGGC TGGGAAACCA 
GGGGTCCCAG GTGAGAGGGG ACTTCCTGGG GCCCATGGAC CCCCTGGACC AACTGGGCCC 
AAGGGTGAGC CGGGTTTCAC GGGTCGCCCT GGAGGACCAG GGGTGGCAGG AGCCCTGGGG 
CAGAAAGGTG ACTTGGGGCT CCCTGGGCAG CCTGGCCTGA GGGGTCCCTC AGGAATCCCA 
GGACTCCAGG GTCCAGCTGG CCCTATTGGG CCCCAAGGCC TGCCGGGCCT GAAGGGGGAA 
CCAGGCCTGC CAGGGCCCCC TGGAGAGGGG AGAGCAGGGG AACCTGGCAC GGCTGGGCCC 
ACGGGGCCCC CAGGGGTCCC TGGCTCCCCT GGAATCACGG GCCCTCCGGG GCCTCCCGGG 
CCCCCGGGAC CCCCTGGTGC CCCTGGGGCC TTCGATGAGA CTGGCATCGC AGGCTTGCAC 
CTGCCCAACG GCGGTGTGGA GGGTGCCGTG CTGGGCAAGG GGGGCAAGCC ACAGTTTGGG 
CTGGGCGAGC TGTCTGCCCA TGCCACACCG GCCTTCACTG CGGTGCTCAC CTCGCCCTTC 
CCCGCCTCGG GCATGCCCGT GAAATTTGAC CGGACTCTCT ACAATGGCCA CAGCGGCTAC 
AACCCAGCCA CTGGCATCTT CACCTGCCCT GTGGGCGGCG TCTACTACTT TGCTTACCAT 
GTGCACGTCA AGGGCACCAA CGTGTGGGTG GCCCTGTACA AGAACAACGT GCCGGCCACC 
TATACCTACG ATGAGTACAA GAAGGGCTAC CTGGACCAGG CATCTGGTGG GGCCGTGCTC 
CAGCTGCGGC CCAACGACCA GGTCTGGGTG CAGATGCCGT CGGACCAGGC CAACGGCCTC 
TACTCCACGG AGTACATCCA CTCCTCCTTT TCAGGATTCT TGCTCTGCCC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL8A2 ( NM_001294347.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001281276.1 Homo sapiens collagen type VIII alpha 2 chain (COL8A2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28708
Clone ID Related Accession (Same CDS sequence)	NM_001294347.1 , NM_001294347.2
Accession Version	NM_001294347.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	collagen alpha-2(VIII) chain isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL138787.11, HY025990.1, AK074129.1 and AI084614.1. On May 31, 2019 this sequence version replaced NM_001294347.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY025990.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA2142348 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCTAC CGCTGCTGCC GATGGACCTG AAGGGAGAGC CCGGCCCCCC TGGGAAGCCC 
GGGCCTCGGG GTCCCCCTGG CCCCCCTGGC TTCCCAGGAA AACCAGGCAT GGGAAAGCCA 
GGACTCCATG GGCAGCCTGG CCCTGCTGGG CCCCCTGGCT TCTCCCGGAT GGGCAAGGCT 
GGTCCCCCAG GGCTCCCTGG CAAGGTCGGG CCACCAGGGC AGCCGGGGCT TCGGGGGGAG 
CCAGGAATAC GAGGGGACCA GGGCCTCCGG GGACCCCCAG GACCCCCTGG CCTCCCGGGC 
CCCTCAGGCA TTACTATCCC TGGAAAACCA GGTGCCCAAG GGGTGCCAGG GCCCCCAGGA 
TTCCAGGGGG AACCAGGGCC CCAGGGGGAG CCTGGGCCCC CAGGTGATCG AGGCCTCAAG 
GGGGATAATG GAGTGGGCCA GCCCGGGCTG CCTGGGGCCC CAGGGCAGGG GGGTGCCCCC 
GGCCCCCCCG GCCTCCCTGG TCCAGCTGGC TTAGGCAAAC CTGGTTTGGA TGGGCTTCCT 
GGGGCCCCAG GAGACAAGGG TGAGTCTGGG CCTCCTGGAG TTCCAGGCCC CAGGGGGGAG 
CCAGGAGCTG TGGGCCCAAA AGGACCTCCT GGAGTAGACG GTGTGGGAGT CCCAGGGGCA 
GCAGGGTTGC CAGGACCACA GGGCCCATCA GGGGCCAAAG GGGAGCCAGG GACCCGGGGC 
CCCCCTGGGC TGATAGGCCC CACTGGCTAT GGGATGCCAG GACTGCCAGG CCCCAAGGGG 
GACAGGGGCC CAGCTGGGGT CCCAGGACTC TTGGGGGACA GGGGTGAGCC AGGGGAGGAT 
GGGGAGCCAG GGGAGCAGGG CCCACAGGGT CTTGGGGGTC CCCCTGGACT TCCTGGGTCT 
GCAGGGCTTC CTGGCAGACG TGGGCCCCCT GGGCCTAAGG GTGAGGCAGG GCCTGGAGGA 
CCCCCAGGAG TGCCTGGCAT TCGAGGTGAC CAGGGGCCTA GTGGCCTGGC TGGGAAACCA 
GGGGTCCCAG GTGAGAGGGG ACTTCCTGGG GCCCATGGAC CCCCTGGACC AACTGGGCCC 
AAGGGTGAGC CGGGTTTCAC GGGTCGCCCT GGAGGACCAG GGGTGGCAGG AGCCCTGGGG 
CAGAAAGGTG ACTTGGGGCT CCCTGGGCAG CCTGGCCTGA GGGGTCCCTC AGGAATCCCA 
GGACTCCAGG GTCCAGCTGG CCCTATTGGG CCCCAAGGCC TGCCGGGCCT GAAGGGGGAA 
CCAGGCCTGC CAGGGCCCCC TGGAGAGGGG AGAGCAGGGG AACCTGGCAC GGCTGGGCCC 
ACGGGGCCCC CAGGGGTCCC TGGCTCCCCT GGAATCACGG GCCCTCCGGG GCCTCCCGGG 
CCCCCGGGAC CCCCTGGTGC CCCTGGGGCC TTCGATGAGA CTGGCATCGC AGGCTTGCAC 
CTGCCCAACG GCGGTGTGGA GGGTGCCGTG CTGGGCAAGG GGGGCAAGCC ACAGTTTGGG 
CTGGGCGAGC TGTCTGCCCA TGCCACACCG GCCTTCACTG CGGTGCTCAC CTCGCCCTTC 
CCCGCCTCGG GCATGCCCGT GAAATTTGAC CGGACTCTCT ACAATGGCCA CAGCGGCTAC 
AACCCAGCCA CTGGCATCTT CACCTGCCCT GTGGGCGGCG TCTACTACTT TGCTTACCAT 
GTGCACGTCA AGGGCACCAA CGTGTGGGTG GCCCTGTACA AGAACAACGT GCCGGCCACC 
TATACCTACG ATGAGTACAA GAAGGGCTAC CTGGACCAGG CATCTGGTGG GGCCGTGCTC 
CAGCTGCGGC CCAACGACCA GGTCTGGGTG CAGATGCCGT CGGACCAGGC CAACGGCCTC 
TACTCCACGG AGTACATCCA CTCCTCCTTT TCAGGATTCT TGCTCTGCCC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001281276.1
CDS	278..2194
Translation	MPLPLLPMDLKGEPGPPGKPGPRGPPGPPGFPGKPGMGKPGLHGQPGPAGPPGFSRMGKAGPPGLPGKVGPPGQPGLRGEPGIRGDQGLRGPPGPPGLPGPSGITIPGKPGAQGVPGPPGFQGEPGPQGEPGPPGDRGLKGDNGVGQPGLPGAPGQGGAPGPPGLPGPAGLGKPGLDGLPGAPGDKGESGPPGVPGPRGEPGAVGPKGPPGVDGVGVPGAAGLPGPQGPSGAKGEPGTRGPPGLIGPTGYGMPGLPGPKGDRGPAGVPGLLGDRGEPGEDGEPGEQGPQGLGGPPGLPGSAGLPGRRGPPGPKGEAGPGGPPGVPGIRGDQGPSGLAGKPGVPGERGLPGAHGPPGPTGPKGEPGFTGRPGGPGVAGALGQKGDLGLPGQPGLRGPSGIPGLQGPAGPIGPQGLPGLKGEPGLPGPPGEGRAGEPGTAGPTGPPGVPGSPGITGPPGPPGPPGPPGAPGAFDETGIAGLHLPNGGVEGAVLGKGGKPQFGLGELSAHATPAFTAVLTSPFPASGMPVKFDRTLYNGHSGYNPATGIFTCPVGGVYYFAYHVHVKGTNVWVALYKNNVPATYTYDEYKKGYLDQASGGAVLQLRPNDQVWVQMPSDQANGLYSTEYIHSSFSGFLLCPT

Target ORF information:

RefSeq Version	NM_001294347.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VIII alpha 2 chain (COL8A2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001294347.2

ORF Insert Sequence:

ATGCCTCTAC CGCTGCTGCC GATGGACCTG AAGGGAGAGC CCGGCCCCCC TGGGAAGCCC 
GGGCCTCGGG GTCCCCCTGG CCCCCCTGGC TTCCCAGGAA AACCAGGCAT GGGAAAGCCA 
GGACTCCATG GGCAGCCTGG CCCTGCTGGG CCCCCTGGCT TCTCCCGGAT GGGCAAGGCT 
GGTCCCCCAG GGCTCCCTGG CAAGGTCGGG CCACCAGGGC AGCCGGGGCT TCGGGGGGAG 
CCAGGAATAC GAGGGGACCA GGGCCTCCGG GGACCCCCAG GACCCCCTGG CCTCCCGGGC 
CCCTCAGGCA TTACTATCCC TGGAAAACCA GGTGCCCAAG GGGTGCCAGG GCCCCCAGGA 
TTCCAGGGGG AACCAGGGCC CCAGGGGGAG CCTGGGCCCC CAGGTGATCG AGGCCTCAAG 
GGGGATAATG GAGTGGGCCA GCCCGGGCTG CCTGGGGCCC CAGGGCAGGG GGGTGCCCCC 
GGCCCCCCCG GCCTCCCTGG TCCAGCTGGC TTAGGCAAAC CTGGTTTGGA TGGGCTTCCT 
GGGGCCCCAG GAGACAAGGG TGAGTCTGGG CCTCCTGGAG TTCCAGGCCC CAGGGGGGAG 
CCAGGAGCTG TGGGCCCAAA AGGACCTCCT GGAGTAGACG GTGTGGGAGT CCCAGGGGCA 
GCAGGGTTGC CAGGACCACA GGGCCCATCA GGGGCCAAAG GGGAGCCAGG GACCCGGGGC 
CCCCCTGGGC TGATAGGCCC CACTGGCTAT GGGATGCCAG GACTGCCAGG CCCCAAGGGG 
GACAGGGGCC CAGCTGGGGT CCCAGGACTC TTGGGGGACA GGGGTGAGCC AGGGGAGGAT 
GGGGAGCCAG GGGAGCAGGG CCCACAGGGT CTTGGGGGTC CCCCTGGACT TCCTGGGTCT 
GCAGGGCTTC CTGGCAGACG TGGGCCCCCT GGGCCTAAGG GTGAGGCAGG GCCTGGAGGA 
CCCCCAGGAG TGCCTGGCAT TCGAGGTGAC CAGGGGCCTA GTGGCCTGGC TGGGAAACCA 
GGGGTCCCAG GTGAGAGGGG ACTTCCTGGG GCCCATGGAC CCCCTGGACC AACTGGGCCC 
AAGGGTGAGC CGGGTTTCAC GGGTCGCCCT GGAGGACCAG GGGTGGCAGG AGCCCTGGGG 
CAGAAAGGTG ACTTGGGGCT CCCTGGGCAG CCTGGCCTGA GGGGTCCCTC AGGAATCCCA 
GGACTCCAGG GTCCAGCTGG CCCTATTGGG CCCCAAGGCC TGCCGGGCCT GAAGGGGGAA 
CCAGGCCTGC CAGGGCCCCC TGGAGAGGGG AGAGCAGGGG AACCTGGCAC GGCTGGGCCC 
ACGGGGCCCC CAGGGGTCCC TGGCTCCCCT GGAATCACGG GCCCTCCGGG GCCTCCCGGG 
CCCCCGGGAC CCCCTGGTGC CCCTGGGGCC TTCGATGAGA CTGGCATCGC AGGCTTGCAC 
CTGCCCAACG GCGGTGTGGA GGGTGCCGTG CTGGGCAAGG GGGGCAAGCC ACAGTTTGGG 
CTGGGCGAGC TGTCTGCCCA TGCCACACCG GCCTTCACTG CGGTGCTCAC CTCGCCCTTC 
CCCGCCTCGG GCATGCCCGT GAAATTTGAC CGGACTCTCT ACAATGGCCA CAGCGGCTAC 
AACCCAGCCA CTGGCATCTT CACCTGCCCT GTGGGCGGCG TCTACTACTT TGCTTACCAT 
GTGCACGTCA AGGGCACCAA CGTGTGGGTG GCCCTGTACA AGAACAACGT GCCGGCCACC 
TATACCTACG ATGAGTACAA GAAGGGCTAC CTGGACCAGG CATCTGGTGG GGCCGTGCTC 
CAGCTGCGGC CCAACGACCA GGTCTGGGTG CAGATGCCGT CGGACCAGGC CAACGGCCTC 
TACTCCACGG AGTACATCCA CTCCTCCTTT TCAGGATTCT TGCTCTGCCC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL8A2 ( NM_005202.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005193.1 Homo sapiens collagen type VIII alpha 2 chain (COL8A2), transcript variant 1, mRNA.

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CloneID	OHu28719
Clone ID Related Accession (Same CDS sequence)	NM_005202.3 , NM_005202.4
Accession Version	NM_005202.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-25
Organism	Homo sapiens(human)
Product	collagen alpha-2(VIII) chain isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY025990.1, AK074129.1, AL138787.11 and AI084614.1. This sequence is a reference standard in the RefSeqGene project. On or before Jul 1, 2014 this sequence version replaced XM_005270478.2, NM_005202.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK074129.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA2142348 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGGGGA CTCTGACACC CCTGTCTTCG CTGCTGCTGC TGCTACTGGT GCTGGTGCTG 
GGGTGTGGGC CGCGGGCGTC CTCTGGTGGC GGGGCCGGTG GGGCGGCGGG CTATGCCCCA 
GTGAAGTACA TCCAGCCCAT GCAGAAAGGA CCTGTGGGAC CGCCCTTCCG TGAGGGCAAA 
GGCCAGTACC TGGAAATGCC TCTACCGCTG CTGCCGATGG ACCTGAAGGG AGAGCCCGGC 
CCCCCTGGGA AGCCCGGGCC TCGGGGTCCC CCTGGCCCCC CTGGCTTCCC AGGAAAACCA 
GGCATGGGAA AGCCAGGACT CCATGGGCAG CCTGGCCCTG CTGGGCCCCC TGGCTTCTCC 
CGGATGGGCA AGGCTGGTCC CCCAGGGCTC CCTGGCAAGG TCGGGCCACC AGGGCAGCCG 
GGGCTTCGGG GGGAGCCAGG AATACGAGGG GACCAGGGCC TCCGGGGACC CCCAGGACCC 
CCTGGCCTCC CGGGCCCCTC AGGCATTACT ATCCCTGGAA AACCAGGTGC CCAAGGGGTG 
CCAGGGCCCC CAGGATTCCA GGGGGAACCA GGGCCCCAGG GGGAGCCTGG GCCCCCAGGT 
GATCGAGGCC TCAAGGGGGA TAATGGAGTG GGCCAGCCCG GGCTGCCTGG GGCCCCAGGG 
CAGGGGGGTG CCCCCGGCCC CCCCGGCCTC CCTGGTCCAG CTGGCTTAGG CAAACCTGGT 
TTGGATGGGC TTCCTGGGGC CCCAGGAGAC AAGGGTGAGT CTGGGCCTCC TGGAGTTCCA 
GGCCCCAGGG GGGAGCCAGG AGCTGTGGGC CCAAAAGGAC CTCCTGGAGT AGACGGTGTG 
GGAGTCCCAG GGGCAGCAGG GTTGCCAGGA CCACAGGGCC CATCAGGGGC CAAAGGGGAG 
CCAGGGACCC GGGGCCCCCC TGGGCTGATA GGCCCCACTG GCTATGGGAT GCCAGGACTG 
CCAGGCCCCA AGGGGGACAG GGGCCCAGCT GGGGTCCCAG GACTCTTGGG GGACAGGGGT 
GAGCCAGGGG AGGATGGGGA GCCAGGGGAG CAGGGCCCAC AGGGTCTTGG GGGTCCCCCT 
GGACTTCCTG GGTCTGCAGG GCTTCCTGGC AGACGTGGGC CCCCTGGGCC TAAGGGTGAG 
GCAGGGCCTG GAGGACCCCC AGGAGTGCCT GGCATTCGAG GTGACCAGGG GCCTAGTGGC 
CTGGCTGGGA AACCAGGGGT CCCAGGTGAG AGGGGACTTC CTGGGGCCCA TGGACCCCCT 
GGACCAACTG GGCCCAAGGG TGAGCCGGGT TTCACGGGTC GCCCTGGAGG ACCAGGGGTG 
GCAGGAGCCC TGGGGCAGAA AGGTGACTTG GGGCTCCCTG GGCAGCCTGG CCTGAGGGGT 
CCCTCAGGAA TCCCAGGACT CCAGGGTCCA GCTGGCCCTA TTGGGCCCCA AGGCCTGCCG 
GGCCTGAAGG GGGAACCAGG CCTGCCAGGG CCCCCTGGAG AGGGGAGAGC AGGGGAACCT 
GGCACGGCTG GGCCCACGGG GCCCCCAGGG GTCCCTGGCT CCCCTGGAAT CACGGGCCCT 
CCGGGGCCTC CCGGGCCCCC GGGACCCCCT GGTGCCCCTG GGGCCTTCGA TGAGACTGGC 
ATCGCAGGCT TGCACCTGCC CAACGGCGGT GTGGAGGGTG CCGTGCTGGG CAAGGGGGGC 
AAGCCACAGT TTGGGCTGGG CGAGCTGTCT GCCCATGCCA CACCGGCCTT CACTGCGGTG 
CTCACCTCGC CCTTCCCCGC CTCGGGCATG CCCGTGAAAT TTGACCGGAC TCTCTACAAT 
GGCCACAGCG GCTACAACCC AGCCACTGGC ATCTTCACCT GCCCTGTGGG CGGCGTCTAC 
TACTTTGCTT ACCATGTGCA CGTCAAGGGC ACCAACGTGT GGGTGGCCCT GTACAAGAAC 
AACGTGCCGG CCACCTATAC CTACGATGAG TACAAGAAGG GCTACCTGGA CCAGGCATCT 
GGTGGGGCCG TGCTCCAGCT GCGGCCCAAC GACCAGGTCT GGGTGCAGAT GCCGTCGGAC 
CAGGCCAACG GCCTCTACTC CACGGAGTAC ATCCACTCCT CCTTTTCAGG ATTCTTGCTC 
TGCCCCACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005193.1
CDS	90..2201
Translation	MLGTLTPLSSLLLLLLVLVLGCGPRASSGGGAGGAAGYAPVKYIQPMQKGPVGPPFREGKGQYLEMPLPLLPMDLKGEPGPPGKPGPRGPPGPPGFPGKPGMGKPGLHGQPGPAGPPGFSRMGKAGPPGLPGKVGPPGQPGLRGEPGIRGDQGLRGPPGPPGLPGPSGITIPGKPGAQGVPGPPGFQGEPGPQGEPGPPGDRGLKGDNGVGQPGLPGAPGQGGAPGPPGLPGPAGLGKPGLDGLPGAPGDKGESGPPGVPGPRGEPGAVGPKGPPGVDGVGVPGAAGLPGPQGPSGAKGEPGTRGPPGLIGPTGYGMPGLPGPKGDRGPAGVPGLLGDRGEPGEDGEPGEQGPQGLGGPPGLPGSAGLPGRRGPPGPKGEAGPGGPPGVPGIRGDQGPSGLAGKPGVPGERGLPGAHGPPGPTGPKGEPGFTGRPGGPGVAGALGQKGDLGLPGQPGLRGPSGIPGLQGPAGPIGPQGLPGLKGEPGLPGPPGEGRAGEPGTAGPTGPPGVPGSPGITGPPGPPGPPGPPGAPGAFDETGIAGLHLPNGGVEGAVLGKGGKPQFGLGELSAHATPAFTAVLTSPFPASGMPVKFDRTLYNGHSGYNPATGIFTCPVGGVYYFAYHVHVKGTNVWVALYKNNVPATYTYDEYKKGYLDQASGGAVLQLRPNDQVWVQMPSDQANGLYSTEYIHSSFSGFLLCPT

Target ORF information:

RefSeq Version	NM_005202.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VIII alpha 2 chain (COL8A2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005202.3

ORF Insert Sequence:

ATGCTGGGGA CTCTGACACC CCTGTCTTCG CTGCTGCTGC TGCTACTGGT GCTGGTGCTG 
GGGTGTGGGC CGCGGGCGTC CTCTGGTGGC GGGGCCGGTG GGGCGGCGGG CTATGCCCCA 
GTGAAGTACA TCCAGCCCAT GCAGAAAGGA CCTGTGGGAC CGCCCTTCCG TGAGGGCAAA 
GGCCAGTACC TGGAAATGCC TCTACCGCTG CTGCCGATGG ACCTGAAGGG AGAGCCCGGC 
CCCCCTGGGA AGCCCGGGCC TCGGGGTCCC CCTGGCCCCC CTGGCTTCCC AGGAAAACCA 
GGCATGGGAA AGCCAGGACT CCATGGGCAG CCTGGCCCTG CTGGGCCCCC TGGCTTCTCC 
CGGATGGGCA AGGCTGGTCC CCCAGGGCTC CCTGGCAAGG TCGGGCCACC AGGGCAGCCG 
GGGCTTCGGG GGGAGCCAGG AATACGAGGG GACCAGGGCC TCCGGGGACC CCCAGGACCC 
CCTGGCCTCC CGGGCCCCTC AGGCATTACT ATCCCTGGAA AACCAGGTGC CCAAGGGGTG 
CCAGGGCCCC CAGGATTCCA GGGGGAACCA GGGCCCCAGG GGGAGCCTGG GCCCCCAGGT 
GATCGAGGCC TCAAGGGGGA TAATGGAGTG GGCCAGCCCG GGCTGCCTGG GGCCCCAGGG 
CAGGGGGGTG CCCCCGGCCC CCCCGGCCTC CCTGGTCCAG CTGGCTTAGG CAAACCTGGT 
TTGGATGGGC TTCCTGGGGC CCCAGGAGAC AAGGGTGAGT CTGGGCCTCC TGGAGTTCCA 
GGCCCCAGGG GGGAGCCAGG AGCTGTGGGC CCAAAAGGAC CTCCTGGAGT AGACGGTGTG 
GGAGTCCCAG GGGCAGCAGG GTTGCCAGGA CCACAGGGCC CATCAGGGGC CAAAGGGGAG 
CCAGGGACCC GGGGCCCCCC TGGGCTGATA GGCCCCACTG GCTATGGGAT GCCAGGACTG 
CCAGGCCCCA AGGGGGACAG GGGCCCAGCT GGGGTCCCAG GACTCTTGGG GGACAGGGGT 
GAGCCAGGGG AGGATGGGGA GCCAGGGGAG CAGGGCCCAC AGGGTCTTGG GGGTCCCCCT 
GGACTTCCTG GGTCTGCAGG GCTTCCTGGC AGACGTGGGC CCCCTGGGCC TAAGGGTGAG 
GCAGGGCCTG GAGGACCCCC AGGAGTGCCT GGCATTCGAG GTGACCAGGG GCCTAGTGGC 
CTGGCTGGGA AACCAGGGGT CCCAGGTGAG AGGGGACTTC CTGGGGCCCA TGGACCCCCT 
GGACCAACTG GGCCCAAGGG TGAGCCGGGT TTCACGGGTC GCCCTGGAGG ACCAGGGGTG 
GCAGGAGCCC TGGGGCAGAA AGGTGACTTG GGGCTCCCTG GGCAGCCTGG CCTGAGGGGT 
CCCTCAGGAA TCCCAGGACT CCAGGGTCCA GCTGGCCCTA TTGGGCCCCA AGGCCTGCCG 
GGCCTGAAGG GGGAACCAGG CCTGCCAGGG CCCCCTGGAG AGGGGAGAGC AGGGGAACCT 
GGCACGGCTG GGCCCACGGG GCCCCCAGGG GTCCCTGGCT CCCCTGGAAT CACGGGCCCT 
CCGGGGCCTC CCGGGCCCCC GGGACCCCCT GGTGCCCCTG GGGCCTTCGA TGAGACTGGC 
ATCGCAGGCT TGCACCTGCC CAACGGCGGT GTGGAGGGTG CCGTGCTGGG CAAGGGGGGC 
AAGCCACAGT TTGGGCTGGG CGAGCTGTCT GCCCATGCCA CACCGGCCTT CACTGCGGTG 
CTCACCTCGC CCTTCCCCGC CTCGGGCATG CCCGTGAAAT TTGACCGGAC TCTCTACAAT 
GGCCACAGCG GCTACAACCC AGCCACTGGC ATCTTCACCT GCCCTGTGGG CGGCGTCTAC 
TACTTTGCTT ACCATGTGCA CGTCAAGGGC ACCAACGTGT GGGTGGCCCT GTACAAGAAC 
AACGTGCCGG CCACCTATAC CTACGATGAG TACAAGAAGG GCTACCTGGA CCAGGCATCT 
GGTGGGGCCG TGCTCCAGCT GCGGCCCAAC GACCAGGTCT GGGTGCAGAT GCCGTCGGAC 
CAGGCCAACG GCCTCTACTC CACGGAGTAC ATCCACTCCT CCTTTTCAGG ATTCTTGCTC 
TGCCCCACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL8A2 ( NM_005202.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005193.1 Homo sapiens collagen type VIII alpha 2 chain (COL8A2), transcript variant 1, mRNA.

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CloneID	OHu28719
Clone ID Related Accession (Same CDS sequence)	NM_005202.3 , NM_005202.4
Accession Version	NM_005202.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	collagen alpha-2(VIII) chain isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL138787.11, HY025990.1, AK074129.1 and AI084614.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_005202.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK074129.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA2142348 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000397799.2/ ENSP00000380901.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGGGGA CTCTGACACC CCTGTCTTCG CTGCTGCTGC TGCTACTGGT GCTGGTGCTG 
GGGTGTGGGC CGCGGGCGTC CTCTGGTGGC GGGGCCGGTG GGGCGGCGGG CTATGCCCCA 
GTGAAGTACA TCCAGCCCAT GCAGAAAGGA CCTGTGGGAC CGCCCTTCCG TGAGGGCAAA 
GGCCAGTACC TGGAAATGCC TCTACCGCTG CTGCCGATGG ACCTGAAGGG AGAGCCCGGC 
CCCCCTGGGA AGCCCGGGCC TCGGGGTCCC CCTGGCCCCC CTGGCTTCCC AGGAAAACCA 
GGCATGGGAA AGCCAGGACT CCATGGGCAG CCTGGCCCTG CTGGGCCCCC TGGCTTCTCC 
CGGATGGGCA AGGCTGGTCC CCCAGGGCTC CCTGGCAAGG TCGGGCCACC AGGGCAGCCG 
GGGCTTCGGG GGGAGCCAGG AATACGAGGG GACCAGGGCC TCCGGGGACC CCCAGGACCC 
CCTGGCCTCC CGGGCCCCTC AGGCATTACT ATCCCTGGAA AACCAGGTGC CCAAGGGGTG 
CCAGGGCCCC CAGGATTCCA GGGGGAACCA GGGCCCCAGG GGGAGCCTGG GCCCCCAGGT 
GATCGAGGCC TCAAGGGGGA TAATGGAGTG GGCCAGCCCG GGCTGCCTGG GGCCCCAGGG 
CAGGGGGGTG CCCCCGGCCC CCCCGGCCTC CCTGGTCCAG CTGGCTTAGG CAAACCTGGT 
TTGGATGGGC TTCCTGGGGC CCCAGGAGAC AAGGGTGAGT CTGGGCCTCC TGGAGTTCCA 
GGCCCCAGGG GGGAGCCAGG AGCTGTGGGC CCAAAAGGAC CTCCTGGAGT AGACGGTGTG 
GGAGTCCCAG GGGCAGCAGG GTTGCCAGGA CCACAGGGCC CATCAGGGGC CAAAGGGGAG 
CCAGGGACCC GGGGCCCCCC TGGGCTGATA GGCCCCACTG GCTATGGGAT GCCAGGACTG 
CCAGGCCCCA AGGGGGACAG GGGCCCAGCT GGGGTCCCAG GACTCTTGGG GGACAGGGGT 
GAGCCAGGGG AGGATGGGGA GCCAGGGGAG CAGGGCCCAC AGGGTCTTGG GGGTCCCCCT 
GGACTTCCTG GGTCTGCAGG GCTTCCTGGC AGACGTGGGC CCCCTGGGCC TAAGGGTGAG 
GCAGGGCCTG GAGGACCCCC AGGAGTGCCT GGCATTCGAG GTGACCAGGG GCCTAGTGGC 
CTGGCTGGGA AACCAGGGGT CCCAGGTGAG AGGGGACTTC CTGGGGCCCA TGGACCCCCT 
GGACCAACTG GGCCCAAGGG TGAGCCGGGT TTCACGGGTC GCCCTGGAGG ACCAGGGGTG 
GCAGGAGCCC TGGGGCAGAA AGGTGACTTG GGGCTCCCTG GGCAGCCTGG CCTGAGGGGT 
CCCTCAGGAA TCCCAGGACT CCAGGGTCCA GCTGGCCCTA TTGGGCCCCA AGGCCTGCCG 
GGCCTGAAGG GGGAACCAGG CCTGCCAGGG CCCCCTGGAG AGGGGAGAGC AGGGGAACCT 
GGCACGGCTG GGCCCACGGG GCCCCCAGGG GTCCCTGGCT CCCCTGGAAT CACGGGCCCT 
CCGGGGCCTC CCGGGCCCCC GGGACCCCCT GGTGCCCCTG GGGCCTTCGA TGAGACTGGC 
ATCGCAGGCT TGCACCTGCC CAACGGCGGT GTGGAGGGTG CCGTGCTGGG CAAGGGGGGC 
AAGCCACAGT TTGGGCTGGG CGAGCTGTCT GCCCATGCCA CACCGGCCTT CACTGCGGTG 
CTCACCTCGC CCTTCCCCGC CTCGGGCATG CCCGTGAAAT TTGACCGGAC TCTCTACAAT 
GGCCACAGCG GCTACAACCC AGCCACTGGC ATCTTCACCT GCCCTGTGGG CGGCGTCTAC 
TACTTTGCTT ACCATGTGCA CGTCAAGGGC ACCAACGTGT GGGTGGCCCT GTACAAGAAC 
AACGTGCCGG CCACCTATAC CTACGATGAG TACAAGAAGG GCTACCTGGA CCAGGCATCT 
GGTGGGGCCG TGCTCCAGCT GCGGCCCAAC GACCAGGTCT GGGTGCAGAT GCCGTCGGAC 
CAGGCCAACG GCCTCTACTC CACGGAGTAC ATCCACTCCT CCTTTTCAGG ATTCTTGCTC 
TGCCCCACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005193.1
CDS	228..2339
Translation	MLGTLTPLSSLLLLLLVLVLGCGPRASSGGGAGGAAGYAPVKYIQPMQKGPVGPPFREGKGQYLEMPLPLLPMDLKGEPGPPGKPGPRGPPGPPGFPGKPGMGKPGLHGQPGPAGPPGFSRMGKAGPPGLPGKVGPPGQPGLRGEPGIRGDQGLRGPPGPPGLPGPSGITIPGKPGAQGVPGPPGFQGEPGPQGEPGPPGDRGLKGDNGVGQPGLPGAPGQGGAPGPPGLPGPAGLGKPGLDGLPGAPGDKGESGPPGVPGPRGEPGAVGPKGPPGVDGVGVPGAAGLPGPQGPSGAKGEPGTRGPPGLIGPTGYGMPGLPGPKGDRGPAGVPGLLGDRGEPGEDGEPGEQGPQGLGGPPGLPGSAGLPGRRGPPGPKGEAGPGGPPGVPGIRGDQGPSGLAGKPGVPGERGLPGAHGPPGPTGPKGEPGFTGRPGGPGVAGALGQKGDLGLPGQPGLRGPSGIPGLQGPAGPIGPQGLPGLKGEPGLPGPPGEGRAGEPGTAGPTGPPGVPGSPGITGPPGPPGPPGPPGAPGAFDETGIAGLHLPNGGVEGAVLGKGGKPQFGLGELSAHATPAFTAVLTSPFPASGMPVKFDRTLYNGHSGYNPATGIFTCPVGGVYYFAYHVHVKGTNVWVALYKNNVPATYTYDEYKKGYLDQASGGAVLQLRPNDQVWVQMPSDQANGLYSTEYIHSSFSGFLLCPT

Target ORF information:

RefSeq Version	NM_005202.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VIII alpha 2 chain (COL8A2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005202.4

ORF Insert Sequence:

ATGCTGGGGA CTCTGACACC CCTGTCTTCG CTGCTGCTGC TGCTACTGGT GCTGGTGCTG 
GGGTGTGGGC CGCGGGCGTC CTCTGGTGGC GGGGCCGGTG GGGCGGCGGG CTATGCCCCA 
GTGAAGTACA TCCAGCCCAT GCAGAAAGGA CCTGTGGGAC CGCCCTTCCG TGAGGGCAAA 
GGCCAGTACC TGGAAATGCC TCTACCGCTG CTGCCGATGG ACCTGAAGGG AGAGCCCGGC 
CCCCCTGGGA AGCCCGGGCC TCGGGGTCCC CCTGGCCCCC CTGGCTTCCC AGGAAAACCA 
GGCATGGGAA AGCCAGGACT CCATGGGCAG CCTGGCCCTG CTGGGCCCCC TGGCTTCTCC 
CGGATGGGCA AGGCTGGTCC CCCAGGGCTC CCTGGCAAGG TCGGGCCACC AGGGCAGCCG 
GGGCTTCGGG GGGAGCCAGG AATACGAGGG GACCAGGGCC TCCGGGGACC CCCAGGACCC 
CCTGGCCTCC CGGGCCCCTC AGGCATTACT ATCCCTGGAA AACCAGGTGC CCAAGGGGTG 
CCAGGGCCCC CAGGATTCCA GGGGGAACCA GGGCCCCAGG GGGAGCCTGG GCCCCCAGGT 
GATCGAGGCC TCAAGGGGGA TAATGGAGTG GGCCAGCCCG GGCTGCCTGG GGCCCCAGGG 
CAGGGGGGTG CCCCCGGCCC CCCCGGCCTC CCTGGTCCAG CTGGCTTAGG CAAACCTGGT 
TTGGATGGGC TTCCTGGGGC CCCAGGAGAC AAGGGTGAGT CTGGGCCTCC TGGAGTTCCA 
GGCCCCAGGG GGGAGCCAGG AGCTGTGGGC CCAAAAGGAC CTCCTGGAGT AGACGGTGTG 
GGAGTCCCAG GGGCAGCAGG GTTGCCAGGA CCACAGGGCC CATCAGGGGC CAAAGGGGAG 
CCAGGGACCC GGGGCCCCCC TGGGCTGATA GGCCCCACTG GCTATGGGAT GCCAGGACTG 
CCAGGCCCCA AGGGGGACAG GGGCCCAGCT GGGGTCCCAG GACTCTTGGG GGACAGGGGT 
GAGCCAGGGG AGGATGGGGA GCCAGGGGAG CAGGGCCCAC AGGGTCTTGG GGGTCCCCCT 
GGACTTCCTG GGTCTGCAGG GCTTCCTGGC AGACGTGGGC CCCCTGGGCC TAAGGGTGAG 
GCAGGGCCTG GAGGACCCCC AGGAGTGCCT GGCATTCGAG GTGACCAGGG GCCTAGTGGC 
CTGGCTGGGA AACCAGGGGT CCCAGGTGAG AGGGGACTTC CTGGGGCCCA TGGACCCCCT 
GGACCAACTG GGCCCAAGGG TGAGCCGGGT TTCACGGGTC GCCCTGGAGG ACCAGGGGTG 
GCAGGAGCCC TGGGGCAGAA AGGTGACTTG GGGCTCCCTG GGCAGCCTGG CCTGAGGGGT 
CCCTCAGGAA TCCCAGGACT CCAGGGTCCA GCTGGCCCTA TTGGGCCCCA AGGCCTGCCG 
GGCCTGAAGG GGGAACCAGG CCTGCCAGGG CCCCCTGGAG AGGGGAGAGC AGGGGAACCT 
GGCACGGCTG GGCCCACGGG GCCCCCAGGG GTCCCTGGCT CCCCTGGAAT CACGGGCCCT 
CCGGGGCCTC CCGGGCCCCC GGGACCCCCT GGTGCCCCTG GGGCCTTCGA TGAGACTGGC 
ATCGCAGGCT TGCACCTGCC CAACGGCGGT GTGGAGGGTG CCGTGCTGGG CAAGGGGGGC 
AAGCCACAGT TTGGGCTGGG CGAGCTGTCT GCCCATGCCA CACCGGCCTT CACTGCGGTG 
CTCACCTCGC CCTTCCCCGC CTCGGGCATG CCCGTGAAAT TTGACCGGAC TCTCTACAAT 
GGCCACAGCG GCTACAACCC AGCCACTGGC ATCTTCACCT GCCCTGTGGG CGGCGTCTAC 
TACTTTGCTT ACCATGTGCA CGTCAAGGGC ACCAACGTGT GGGTGGCCCT GTACAAGAAC 
AACGTGCCGG CCACCTATAC CTACGATGAG TACAAGAAGG GCTACCTGGA CCAGGCATCT 
GGTGGGGCCG TGCTCCAGCT GCGGCCCAAC GACCAGGTCT GGGTGCAGAT GCCGTCGGAC 
CAGGCCAACG GCCTCTACTC CACGGAGTAC ATCCACTCCT CCTTTTCAGG ATTCTTGCTC 
TGCCCCACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL8A2 ( XM_005270477.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270534.1 Homo sapiens collagen type VIII alpha 2 chain (COL8A2), transcript variant X1, mRNA.

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CloneID	OHu33026
Clone ID Related Accession (Same CDS sequence)	XM_005270477.3
Accession Version	XM_005270477.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-2(VIII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005270477.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCGGGT CACGACTGGC CCCTGTGAGT CCTCCGGGCT CAGCCTACCG AGGTCACGTC 
TGCAGGCAGC CTTCTGGGGA CTCCTCACTG GGGGCCACCA GCCCGAGCTG CCCCTTCCCT 
GCAGGACCAC GCCTGGCCGT CCTTCTCAGC AGCCGCGTCC GTCCCCACAG GCCCTGCCTC 
TGCTGGAACC TTCCCAGGCC CCTCCGACCT GAAAGCACGT CCACGGACGC CATGCTGGGG 
ACTCTGACAC CCCTGTCTTC GCTGCTGCTG CTGCTACTGG TGCTGGTGCT GGGGTGTGGG 
CCGCGGGCGT CCTCTGGTGG CGGGGCCGGT GGGGCGGCGG GCTATGCCCC AGTGAAGTAC 
ATCCAGCCCA TGCAGAAAGG ACCTGTGGGA CCGCCCTTCC GTGAGGGCAA AGGCCAGTAC 
CTGGAAATGC CTCTACCGCT GCTGCCGATG GACCTGAAGG GAGAGCCCGG CCCCCCTGGG 
AAGCCCGGGC CTCGGGGTCC CCCTGGCCCC CCTGGCTTCC CAGGAAAACC AGGCATGGGA 
AAGCCAGGAC TCCATGGGCA GCCTGGCCCT GCTGGGCCCC CTGGCTTCTC CCGGATGGGC 
AAGGCTGGTC CCCCAGGGCT CCCTGGCAAG GTCGGGCCAC CAGGGCAGCC GGGGCTTCGG 
GGGGAGCCAG GAATACGAGG GGACCAGGGC CTCCGGGGAC CCCCAGGACC CCCTGGCCTC 
CCGGGCCCCT CAGGCATTAC TATCCCTGGA AAACCAGGTG CCCAAGGGGT GCCAGGGCCC 
CCAGGATTCC AGGGGGAACC AGGGCCCCAG GGGGAGCCTG GGCCCCCAGG TGATCGAGGC 
CTCAAGGGGG ATAATGGAGT GGGCCAGCCC GGGCTGCCTG GGGCCCCAGG GCAGGGGGGT 
GCCCCCGGCC CCCCCGGCCT CCCTGGTCCA GCTGGCTTAG GCAAACCTGG TTTGGATGGG 
CTTCCTGGGG CCCCAGGAGA CAAGGGTGAG TCTGGGCCTC CTGGAGTTCC AGGCCCCAGG 
GGGGAGCCAG GAGCTGTGGG CCCAAAAGGA CCTCCTGGAG TAGACGGTGT GGGAGTCCCA 
GGGGCAGCAG GGTTGCCAGG ACCACAGGGC CCATCAGGGG CCAAAGGGGA GCCAGGGACC 
CGGGGCCCCC CTGGGCTGAT AGGCCCCACT GGCTATGGGA TGCCAGGACT GCCAGGCCCC 
AAGGGGGACA GGGGCCCAGC TGGGGTCCCA GGACTCTTGG GGGACAGGGG TGAGCCAGGG 
GAGGATGGGG AGCCAGGGGA GCAGGGCCCA CAGGGTCTTG GGGGTCCCCC TGGACTTCCT 
GGGTCTGCAG GGCTTCCTGG CAGACGTGGG CCCCCTGGGC CTAAGGGTGA GGCAGGGCCT 
GGAGGACCCC CAGGAGTGCC TGGCATTCGA GGTGACCAGG GGCCTAGTGG CCTGGCTGGG 
AAACCAGGGG TCCCAGGTGA GAGGGGACTT CCTGGGGCCC ATGGACCCCC TGGACCAACT 
GGGCCCAAGG GTGAGCCGGG TTTCACGGGT CGCCCTGGAG GACCAGGGGT GGCAGGAGCC 
CTGGGGCAGA AAGGTGACTT GGGGCTCCCT GGGCAGCCTG GCCTGAGGGG TCCCTCAGGA 
ATCCCAGGAC TCCAGGGTCC AGCTGGCCCT ATTGGGCCCC AAGGCCTGCC GGGCCTGAAG 
GGGGAACCAG GCCTGCCAGG GCCCCCTGGA GAGGGGAGAG CAGGGGAACC TGGCACGGCT 
GGGCCCACGG GGCCCCCAGG GGTCCCTGGC TCCCCTGGAA TCACGGGCCC TCCGGGGCCT 
CCCGGGCCCC CGGGACCCCC TGGTGCCCCT GGGGCCTTCG ATGAGACTGG CATCGCAGGC 
TTGCACCTGC CCAACGGCGG TGTGGAGGGT GCCGTGCTGG GCAAGGGGGG CAAGCCACAG 
TTTGGGCTGG GCGAGCTGTC TGCCCATGCC ACACCGGCCT TCACTGCGGT GCTCACCTCG 
CCCTTCCCCG CCTCGGGCAT GCCCGTGAAA TTTGACCGGA CTCTCTACAA TGGCCACAGC 
GGCTACAACC CAGCCACTGG CATCTTCACC TGCCCTGTGG GCGGCGTCTA CTACTTTGCT 
TACCATGTGC ACGTCAAGGG CACCAACGTG TGGGTGGCCC TGTACAAGAA CAACGTGCCG 
GCCACCTATA CCTACGATGA GTACAAGAAG GGCTACCTGG ACCAGGCATC TGGTGGGGCC 
GTGCTCCAGC TGCGGCCCAA CGACCAGGTC TGGGTGCAGA TGCCGTCGGA CCAGGCCAAC 
GGCCTCTACT CCACGGAGTA CATCCACTCC TCCTTTTCAG GATTCTTGCT CTGCCCCACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005270534.1
CDS	559..2901
Translation	MCGSRLAPVSPPGSAYRGHVCRQPSGDSSLGATSPSCPFPAGPRLAVLLSSRVRPHRPCLCWNLPRPLRPESTSTDAMLGTLTPLSSLLLLLLVLVLGCGPRASSGGGAGGAAGYAPVKYIQPMQKGPVGPPFREGKGQYLEMPLPLLPMDLKGEPGPPGKPGPRGPPGPPGFPGKPGMGKPGLHGQPGPAGPPGFSRMGKAGPPGLPGKVGPPGQPGLRGEPGIRGDQGLRGPPGPPGLPGPSGITIPGKPGAQGVPGPPGFQGEPGPQGEPGPPGDRGLKGDNGVGQPGLPGAPGQGGAPGPPGLPGPAGLGKPGLDGLPGAPGDKGESGPPGVPGPRGEPGAVGPKGPPGVDGVGVPGAAGLPGPQGPSGAKGEPGTRGPPGLIGPTGYGMPGLPGPKGDRGPAGVPGLLGDRGEPGEDGEPGEQGPQGLGGPPGLPGSAGLPGRRGPPGPKGEAGPGGPPGVPGIRGDQGPSGLAGKPGVPGERGLPGAHGPPGPTGPKGEPGFTGRPGGPGVAGALGQKGDLGLPGQPGLRGPSGIPGLQGPAGPIGPQGLPGLKGEPGLPGPPGEGRAGEPGTAGPTGPPGVPGSPGITGPPGPPGPPGPPGAPGAFDETGIAGLHLPNGGVEGAVLGKGGKPQFGLGELSAHATPAFTAVLTSPFPASGMPVKFDRTLYNGHSGYNPATGIFTCPVGGVYYFAYHVHVKGTNVWVALYKNNVPATYTYDEYKKGYLDQASGGAVLQLRPNDQVWVQMPSDQANGLYSTEYIHSSFSGFLLCPT

Target ORF information:

RefSeq Version	XM_005270477.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VIII alpha 2 chain (COL8A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270477.3

ORF Insert Sequence:

ATGTGCGGGT CACGACTGGC CCCTGTGAGT CCTCCGGGCT CAGCCTACCG AGGTCACGTC 
TGCAGGCAGC CTTCTGGGGA CTCCTCACTG GGGGCCACCA GCCCGAGCTG CCCCTTCCCT 
GCAGGACCAC GCCTGGCCGT CCTTCTCAGC AGCCGCGTCC GTCCCCACAG GCCCTGCCTC 
TGCTGGAACC TTCCCAGGCC CCTCCGACCT GAAAGCACGT CCACGGACGC CATGCTGGGG 
ACTCTGACAC CCCTGTCTTC GCTGCTGCTG CTGCTACTGG TGCTGGTGCT GGGGTGTGGG 
CCGCGGGCGT CCTCTGGTGG CGGGGCCGGT GGGGCGGCGG GCTATGCCCC AGTGAAGTAC 
ATCCAGCCCA TGCAGAAAGG ACCTGTGGGA CCGCCCTTCC GTGAGGGCAA AGGCCAGTAC 
CTGGAAATGC CTCTACCGCT GCTGCCGATG GACCTGAAGG GAGAGCCCGG CCCCCCTGGG 
AAGCCCGGGC CTCGGGGTCC CCCTGGCCCC CCTGGCTTCC CAGGAAAACC AGGCATGGGA 
AAGCCAGGAC TCCATGGGCA GCCTGGCCCT GCTGGGCCCC CTGGCTTCTC CCGGATGGGC 
AAGGCTGGTC CCCCAGGGCT CCCTGGCAAG GTCGGGCCAC CAGGGCAGCC GGGGCTTCGG 
GGGGAGCCAG GAATACGAGG GGACCAGGGC CTCCGGGGAC CCCCAGGACC CCCTGGCCTC 
CCGGGCCCCT CAGGCATTAC TATCCCTGGA AAACCAGGTG CCCAAGGGGT GCCAGGGCCC 
CCAGGATTCC AGGGGGAACC AGGGCCCCAG GGGGAGCCTG GGCCCCCAGG TGATCGAGGC 
CTCAAGGGGG ATAATGGAGT GGGCCAGCCC GGGCTGCCTG GGGCCCCAGG GCAGGGGGGT 
GCCCCCGGCC CCCCCGGCCT CCCTGGTCCA GCTGGCTTAG GCAAACCTGG TTTGGATGGG 
CTTCCTGGGG CCCCAGGAGA CAAGGGTGAG TCTGGGCCTC CTGGAGTTCC AGGCCCCAGG 
GGGGAGCCAG GAGCTGTGGG CCCAAAAGGA CCTCCTGGAG TAGACGGTGT GGGAGTCCCA 
GGGGCAGCAG GGTTGCCAGG ACCACAGGGC CCATCAGGGG CCAAAGGGGA GCCAGGGACC 
CGGGGCCCCC CTGGGCTGAT AGGCCCCACT GGCTATGGGA TGCCAGGACT GCCAGGCCCC 
AAGGGGGACA GGGGCCCAGC TGGGGTCCCA GGACTCTTGG GGGACAGGGG TGAGCCAGGG 
GAGGATGGGG AGCCAGGGGA GCAGGGCCCA CAGGGTCTTG GGGGTCCCCC TGGACTTCCT 
GGGTCTGCAG GGCTTCCTGG CAGACGTGGG CCCCCTGGGC CTAAGGGTGA GGCAGGGCCT 
GGAGGACCCC CAGGAGTGCC TGGCATTCGA GGTGACCAGG GGCCTAGTGG CCTGGCTGGG 
AAACCAGGGG TCCCAGGTGA GAGGGGACTT CCTGGGGCCC ATGGACCCCC TGGACCAACT 
GGGCCCAAGG GTGAGCCGGG TTTCACGGGT CGCCCTGGAG GACCAGGGGT GGCAGGAGCC 
CTGGGGCAGA AAGGTGACTT GGGGCTCCCT GGGCAGCCTG GCCTGAGGGG TCCCTCAGGA 
ATCCCAGGAC TCCAGGGTCC AGCTGGCCCT ATTGGGCCCC AAGGCCTGCC GGGCCTGAAG 
GGGGAACCAG GCCTGCCAGG GCCCCCTGGA GAGGGGAGAG CAGGGGAACC TGGCACGGCT 
GGGCCCACGG GGCCCCCAGG GGTCCCTGGC TCCCCTGGAA TCACGGGCCC TCCGGGGCCT 
CCCGGGCCCC CGGGACCCCC TGGTGCCCCT GGGGCCTTCG ATGAGACTGG CATCGCAGGC 
TTGCACCTGC CCAACGGCGG TGTGGAGGGT GCCGTGCTGG GCAAGGGGGG CAAGCCACAG 
TTTGGGCTGG GCGAGCTGTC TGCCCATGCC ACACCGGCCT TCACTGCGGT GCTCACCTCG 
CCCTTCCCCG CCTCGGGCAT GCCCGTGAAA TTTGACCGGA CTCTCTACAA TGGCCACAGC 
GGCTACAACC CAGCCACTGG CATCTTCACC TGCCCTGTGG GCGGCGTCTA CTACTTTGCT 
TACCATGTGC ACGTCAAGGG CACCAACGTG TGGGTGGCCC TGTACAAGAA CAACGTGCCG 
GCCACCTATA CCTACGATGA GTACAAGAAG GGCTACCTGG ACCAGGCATC TGGTGGGGCC 
GTGCTCCAGC TGCGGCCCAA CGACCAGGTC TGGGTGCAGA TGCCGTCGGA CCAGGCCAAC 
GGCCTCTACT CCACGGAGTA CATCCACTCC TCCTTTTCAG GATTCTTGCT CTGCCCCACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Journal of proteome research16(8)3083-3091(2017 Aug)
Naba A,Pearce OMT,Del Rosario A,Ma D,Ding H,Rajeeve V,Cutillas PR,Balkwill FR,Hynes RO

Analysis of SLC4A11, ZEB1, LOXHD1, COL8A2 and TCF4 gene sequences in a multi-generational family with late-onset Fuchs corneal dystrophy.
International journal of molecular medicine37(6)1487-500(2016 Jun)
Tang H,Zhang W,Yan XM,Wang LP,Dong H,Shou T,Lei H,Guo Q

Distinct Clinical Phenotype of Corneal Dystrophy Predicts the p.(Leu450Trp) Substitution in COL8A2.
Cornea35(5)587-91(2016 May)
Eghrari AO,Riazuddin SA,Gottsch JD

Biosynthetic and functional defects in newly identified SLC4A11 mutants and absence of COL8A2 mutations in Fuchs endothelial corneal dystrophy.
Journal of human genetics59(8)444-53(2014 Aug)
Soumittra N,Loganathan SK,Madhavan D,Ramprasad VL,Arokiasamy T,Sumathi S,Karthiyayini T,Rachapalli SR,Kumaramanickavel G,Casey JR,Rajagopal R

Extracellular matrix signatures of human primary metastatic colon cancers and their metastases to liver.
BMC cancer14518(2014 Jul)
Naba A,Clauser KR,Whittaker CA,Carr SA,Tanabe KK,Hynes RO

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COL8A2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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