gRNAs
The following Col8a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col8a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu74842 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017320266.2 , XM_017320266.1 | |
| Accession Version | XM_017320266.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | collagen alpha-2(VIII) chain isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320266.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGGGCAA GACACCACTT TGTATCCGGA AGCCCATCCA CAGACGCCAT GCAGGGGGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017175755.1 |
| CDS | 784..2931 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_017320266.2 |
1 | ATGGGGGCAA GACACCACTT TGTATCCGGA AGCCCATCCA CAGACGCCAT GCAGGGGGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu16006 | |
| Clone ID Related Accession (Same CDS sequence) | NM_199473.2 | |
| Accession Version | NM_199473.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-03 | |
| Organism | Mus musculus(house mouse) | |
| Product | collagen alpha-2(VIII) chain precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL606976.25. On Nov 17, 2006 this sequence version replaced NM_199473.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065148.1, BB611994.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGCAGGGGG CTCTGATGCC CCTGCCTTCG CTGCTGCTGT TGCTGTTGGG GTGTGGGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_955767.1 |
| CDS | 200..2299 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_199473.2 |
1 | ATGCAGGGGG CTCTGATGCC CCTGCCTTCG CTGCTGCTGT TGCTGTTGGG GTGTGGGCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu74842 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017320266.2 , XM_017320266.1 | |
| Accession Version | XM_017320266.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | collagen alpha-2(VIII) chain isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGGGCAA GACACCACTT TGTATCCGGA AGCCCATCCA CAGACGCCAT GCAGGGGGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017175755.1 |
| CDS | 857..3004 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017320266.1 |
1 | ATGGGGGCAA GACACCACTT TGTATCCGGA AGCCCATCCA CAGACGCCAT GCAGGGGGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Lack of collagen VIII reduces fibrosis and promotes early mortality and cardiac dilatation in pressure overload in mice. |
N-Acetylcysteine increases corneal endothelial cell survival in a mouse model of Fuchs endothelial corneal dystrophy. |
Type VIII collagen signals via ?1 integrin and RhoA to regulate MMP-2 expression and smooth muscle cell migration. |
Type VIII collagen mediates vessel wall remodeling after arterial injury and fibrous cap formation in atherosclerosis. |
Scleral structural alterations associated with chronic experimental intraocular pressure elevation in mice. |
