CMA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CMA1
Entrez Gene ID	1215
Full Name	chymase 1
Synonyms	CYH,MCT1,chymase
General protein information	Preferred Names chymase 1 Names chymase alpha-chymase chymase 1 preproprotein transcript E chymase 1 preproprotein transcript I chymase 1, mast cell chymase, heart chymase, mast cell mast cell protease I
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q12
Summary	This gene encodes a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and is thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Alternative splicing results in multiple variants. [provided by RefSeq, Apr 2015].
Disorder MIM:	118938

mRNA and Protein(s)

mRNA	Protein	Name
NM_001308083.2	NP_001295012.1	chymase isoform 2
NM_001836.5	NP_001827.1	chymase isoform 1 preproprotein
NM_001836.4	NP_001827.1	chymase isoform 1 preproprotein
NM_001308083.1	NP_001295012.1	chymase isoform 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC540321	XP_002696732.1
Rattus norvegicus (Norway rat)	Cma1	NP_037224.1
Bos taurus (cattle)	LOC100139881	XP_002696686.1
Homo sapiens (human)	CMA1	NP_001827.1
Pan troglodytes (chimpanzee)	CMA1	XP_001170224.1
	CMA1	XP_001114321.1
Canis lupus familiaris (dog)	CMA1	NP_001013442.1
Mus musculus (house mouse)	Cma1	NP_034910.1

Gene Ontology
Bibliography

Related articles in PubMed

Upregulation of cathepsin C expression contributes to endothelial chymase activation in preeclampsia.
Gu Y, Lewis DF, Alexander JS, Wang Y
Hypertension research : official journal of the Japanese Society of Hypertension40(12)976-981(2017 Dec)

Hypoxia-induced angiotensin II by the lactate-chymase-dependent mechanism mediates radioresistance of hypoxic tumor cells.
Xie G, Liu Y, Yao Q, Zheng R, Zhang L, Lin J, Guo Z, Du S, Ren C, Yuan Q, Yuan Y
Scientific reports742396(2017 Feb)

Highly Selective Cleavage of Cytokines and Chemokines by the Human Mast Cell Chymase and Neutrophil Cathepsin G.
Fu Z, Thorpe M, Alemayehu R, Roy A, Kervinen J, de Garavilla L, ?brink M, Hellman L
Journal of immunology (Baltimore, Md. : 1950)198(4)1474-1483(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cleavage of the alarmins by Human mast cell chymase and human neutrophil cathepsin G suggests a function in regulating excessive inflammation.
Title: Highly Selective Cleavage of Cytokines and Chemokines by the Human Mast Cell Chymase and Neutrophil Cathepsin G.

promoter single nucleotide polymorphism is not associated with Dengue Hemorrhagic Fever and Dengue Shock Syndrome in Vietnam and Philippines
Title: Alpha tryptase allele of Tryptase 1 (TPSAB1) gene associated with Dengue Hemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS) in Vietnam and Philippines.

CMA1 gene single nucleotide polymorphisms is associated with essential hypertension.
Title: [Association of CMA1 gene tag single nucleotide polymorphisms with essential hypertension in Yi population from Yunnan].

Our data demonstrated that mast cell chymase plays an important role in keloid formation through TGF-beta1/Smad signaling pathway.
Title: Mast cell chymase in keloid induces profibrotic response via transforming growth factor-?1/Smad activation in keloid fibroblasts.

Significant association between the AG genotype of CMA1 A polymorphism and Angina Pectoris and Ventricular Extrasystoles was observed.
Title: [Renin-angiotensin-aldosterone system in hypertrophic cardiomyopathy].

The following CMA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CMA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27031	NM_001836.5 Latest version!	Homo sapiens chymase 1 (CMA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu56952	NM_001308083.2 Latest version!	Homo sapiens chymase 1 (CMA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OHu27031	NM_001836.4	Homo sapiens chymase 1 (CMA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu56952	NM_001308083.1	Homo sapiens chymase 1 (CMA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CMA1 ( NM_001836.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001827.1 Homo sapiens chymase 1 (CMA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27031
Clone ID Related Accession (Same CDS sequence)	NM_001836.5 , NM_001836.4
Accession Version	NM_001836.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	chymase isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC069110.1, DQ082729.1, HY275608.1 and AL132800.4. On Nov 23, 2018 this sequence version replaced NM_001836.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.145526.1, M69136.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000250378.7/ ENSP00000250378.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGCTTC TTCCTCTCCC CCTGCTGCTC TTTCTCTTGT GCTCCAGAGC TGAAGCTGGG 
GAGATCATCG GGGGCACAGA ATGCAAGCCA CATTCCCGCC CCTACATGGC CTACCTGGAA 
ATTGTAACTT CCAACGGTCC CTCAAAATTT TGTGGTGGTT TCCTTATAAG ACGGAACTTT 
GTGCTGACGG CTGCTCATTG TGCAGGAAGG TCTATAACAG TCACCCTTGG AGCCCATAAC 
ATAACAGAGG AAGAAGACAC ATGGCAGAAG CTTGAGGTTA TAAAGCAATT CCGTCATCCA 
AAATATAACA CTTCTACTCT TCACCACGAT ATCATGTTAC TAAAGTTGAA GGAGAAAGCC 
AGCCTGACCC TGGCTGTGGG GACACTCCCC TTCCCATCCC AATTCAACTT TGTCCCACCT 
GGGAGAATGT GCCGGGTGGC TGGCTGGGGA AGAACAGGTG TGTTGAAGCC GGGCTCAGAC 
ACTCTGCAAG AGGTGAAGCT GAGACTCATG GATCCCCAGG CCTGCAGCCA CTTCAGAGAC 
TTTGACCACA ATCTTCAGCT GTGTGTGGGC AATCCCAGGA AGACAAAATC TGCATTTAAG 
GGAGACTCTG GGGGCCCTCT TCTGTGTGCT GGGGTGGCCC AGGGCATCGT ATCCTATGGA 
CGGTCGGATG CAAAGCCCCC TGCTGTCTTC ACCCGAATCT CCCATTACCG GCCCTGGATC 
AACCAGATCC TGCAGGCAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001827.1
CDS	31..774
Translation	MLLLPLPLLLFLLCSRAEAGEIIGGTECKPHSRPYMAYLEIVTSNGPSKFCGGFLIRRNFVLTAAHCAGRSITVTLGAHNITEEEDTWQKLEVIKQFRHPKYNTSTLHHDIMLLKLKEKASLTLAVGTLPFPSQFNFVPPGRMCRVAGWGRTGVLKPGSDTLQEVKLRLMDPQACSHFRDFDHNLQLCVGNPRKTKSAFKGDSGGPLLCAGVAQGIVSYGRSDAKPPAVFTRISHYRPWINQILQAN

Target ORF information:

RefSeq Version	NM_001836.5
Organism	Homo sapiens(human)
Definition	Homo sapiens chymase 1 (CMA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001836.5

ORF Insert Sequence:

ATGCTGCTTC TTCCTCTCCC CCTGCTGCTC TTTCTCTTGT GCTCCAGAGC TGAAGCTGGG 
GAGATCATCG GGGGCACAGA ATGCAAGCCA CATTCCCGCC CCTACATGGC CTACCTGGAA 
ATTGTAACTT CCAACGGTCC CTCAAAATTT TGTGGTGGTT TCCTTATAAG ACGGAACTTT 
GTGCTGACGG CTGCTCATTG TGCAGGAAGG TCTATAACAG TCACCCTTGG AGCCCATAAC 
ATAACAGAGG AAGAAGACAC ATGGCAGAAG CTTGAGGTTA TAAAGCAATT CCGTCATCCA 
AAATATAACA CTTCTACTCT TCACCACGAT ATCATGTTAC TAAAGTTGAA GGAGAAAGCC 
AGCCTGACCC TGGCTGTGGG GACACTCCCC TTCCCATCCC AATTCAACTT TGTCCCACCT 
GGGAGAATGT GCCGGGTGGC TGGCTGGGGA AGAACAGGTG TGTTGAAGCC GGGCTCAGAC 
ACTCTGCAAG AGGTGAAGCT GAGACTCATG GATCCCCAGG CCTGCAGCCA CTTCAGAGAC 
TTTGACCACA ATCTTCAGCT GTGTGTGGGC AATCCCAGGA AGACAAAATC TGCATTTAAG 
GGAGACTCTG GGGGCCCTCT TCTGTGTGCT GGGGTGGCCC AGGGCATCGT ATCCTATGGA 
CGGTCGGATG CAAAGCCCCC TGCTGTCTTC ACCCGAATCT CCCATTACCG GCCCTGGATC 
AACCAGATCC TGCAGGCAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CMA1 ( NM_001836.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001827.1 Homo sapiens chymase 1 (CMA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27031
Clone ID Related Accession (Same CDS sequence)	NM_001836.5 , NM_001836.4
Accession Version	NM_001836.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	chymase isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC069110.1, DQ082729.1 and HY275608.1. On Apr 28, 2015 this sequence version replaced NM_001836.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC069110.1, SRR1163655.145526.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTTC TTCCTCTCCC CCTGCTGCTC TTTCTCTTGT GCTCCAGAGC TGAAGCTGGG 
GAGATCATCG GGGGCACAGA ATGCAAGCCA CATTCCCGCC CCTACATGGC CTACCTGGAA 
ATTGTAACTT CCAACGGTCC CTCAAAATTT TGTGGTGGTT TCCTTATAAG ACGGAACTTT 
GTGCTGACGG CTGCTCATTG TGCAGGAAGG TCTATAACAG TCACCCTTGG AGCCCATAAC 
ATAACAGAGG AAGAAGACAC ATGGCAGAAG CTTGAGGTTA TAAAGCAATT CCGTCATCCA 
AAATATAACA CTTCTACTCT TCACCACGAT ATCATGTTAC TAAAGTTGAA GGAGAAAGCC 
AGCCTGACCC TGGCTGTGGG GACACTCCCC TTCCCATCCC AATTCAACTT TGTCCCACCT 
GGGAGAATGT GCCGGGTGGC TGGCTGGGGA AGAACAGGTG TGTTGAAGCC GGGCTCAGAC 
ACTCTGCAAG AGGTGAAGCT GAGACTCATG GATCCCCAGG CCTGCAGCCA CTTCAGAGAC 
TTTGACCACA ATCTTCAGCT GTGTGTGGGC AATCCCAGGA AGACAAAATC TGCATTTAAG 
GGAGACTCTG GGGGCCCTCT TCTGTGTGCT GGGGTGGCCC AGGGCATCGT ATCCTATGGA 
CGGTCGGATG CAAAGCCCCC TGCTGTCTTC ACCCGAATCT CCCATTACCG GCCCTGGATC 
AACCAGATCC TGCAGGCAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001827.1
CDS	31..774
Translation	MLLLPLPLLLFLLCSRAEAGEIIGGTECKPHSRPYMAYLEIVTSNGPSKFCGGFLIRRNFVLTAAHCAGRSITVTLGAHNITEEEDTWQKLEVIKQFRHPKYNTSTLHHDIMLLKLKEKASLTLAVGTLPFPSQFNFVPPGRMCRVAGWGRTGVLKPGSDTLQEVKLRLMDPQACSHFRDFDHNLQLCVGNPRKTKSAFKGDSGGPLLCAGVAQGIVSYGRSDAKPPAVFTRISHYRPWINQILQAN

Target ORF information:

RefSeq Version	NM_001836.4
Organism	Homo sapiens(human)
Definition	Homo sapiens chymase 1 (CMA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001836.4

ORF Insert Sequence:

ATGCTGCTTC TTCCTCTCCC CCTGCTGCTC TTTCTCTTGT GCTCCAGAGC TGAAGCTGGG 
GAGATCATCG GGGGCACAGA ATGCAAGCCA CATTCCCGCC CCTACATGGC CTACCTGGAA 
ATTGTAACTT CCAACGGTCC CTCAAAATTT TGTGGTGGTT TCCTTATAAG ACGGAACTTT 
GTGCTGACGG CTGCTCATTG TGCAGGAAGG TCTATAACAG TCACCCTTGG AGCCCATAAC 
ATAACAGAGG AAGAAGACAC ATGGCAGAAG CTTGAGGTTA TAAAGCAATT CCGTCATCCA 
AAATATAACA CTTCTACTCT TCACCACGAT ATCATGTTAC TAAAGTTGAA GGAGAAAGCC 
AGCCTGACCC TGGCTGTGGG GACACTCCCC TTCCCATCCC AATTCAACTT TGTCCCACCT 
GGGAGAATGT GCCGGGTGGC TGGCTGGGGA AGAACAGGTG TGTTGAAGCC GGGCTCAGAC 
ACTCTGCAAG AGGTGAAGCT GAGACTCATG GATCCCCAGG CCTGCAGCCA CTTCAGAGAC 
TTTGACCACA ATCTTCAGCT GTGTGTGGGC AATCCCAGGA AGACAAAATC TGCATTTAAG 
GGAGACTCTG GGGGCCCTCT TCTGTGTGCT GGGGTGGCCC AGGGCATCGT ATCCTATGGA 
CGGTCGGATG CAAAGCCCCC TGCTGTCTTC ACCCGAATCT CCCATTACCG GCCCTGGATC 
AACCAGATCC TGCAGGCAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CMA1 ( NM_001308083.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001295012.1 Homo sapiens chymase 1 (CMA1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu56952
Clone ID Related Accession (Same CDS sequence)	NM_001308083.2 , NM_001308083.1
Accession Version	NM_001308083.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	chymase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC103974.1, DQ082729.1, HY275608.1 and AL132800.4. On May 31, 2019 this sequence version replaced NM_001308083.1. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an exon in the 5' coding region, which results in use of a downstream start codon compared to variant 1. It encodes isoform 2, which has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC103974.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2147975 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTACTAA AGTTGAAGGA GAAAGCCAGC CTGACCCTGG CTGTGGGGAC ACTCCCCTTC 
CCATCCCAAT TCAACTTTGT CCCACCTGGG AGAATGTGCC GGGTGGCTGG CTGGGGAAGA 
ACAGGTGTGT TGAAGCCGGG CTCAGACACT CTGCAAGAGG TGAAGCTGAG ACTCATGGAT 
CCCCAGGCCT GCAGCCACTT CAGAGACTTT GACCACAATC TTCAGCTGTG TGTGGGCAAT 
CCCAGGAAGA CAAAATCTGC ATTTAAGGGA GACTCTGGGG GCCCTCTTCT GTGTGCTGGG 
GTGGCCCAGG GCATCGTATC CTATGGACGG TCGGATGCAA AGCCCCCTGC TGTCTTCACC 
CGAATCTCCC ATTACCGGCC CTGGATCAAC CAGATCCTGC AGGCAAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295012.1
CDS	213..623
Translation	MLLKLKEKASLTLAVGTLPFPSQFNFVPPGRMCRVAGWGRTGVLKPGSDTLQEVKLRLMDPQACSHFRDFDHNLQLCVGNPRKTKSAFKGDSGGPLLCAGVAQGIVSYGRSDAKPPAVFTRISHYRPWINQILQAN

Target ORF information:

RefSeq Version	NM_001308083.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chymase 1 (CMA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308083.2

ORF Insert Sequence:

ATGTTACTAA AGTTGAAGGA GAAAGCCAGC CTGACCCTGG CTGTGGGGAC ACTCCCCTTC 
CCATCCCAAT TCAACTTTGT CCCACCTGGG AGAATGTGCC GGGTGGCTGG CTGGGGAAGA 
ACAGGTGTGT TGAAGCCGGG CTCAGACACT CTGCAAGAGG TGAAGCTGAG ACTCATGGAT 
CCCCAGGCCT GCAGCCACTT CAGAGACTTT GACCACAATC TTCAGCTGTG TGTGGGCAAT 
CCCAGGAAGA CAAAATCTGC ATTTAAGGGA GACTCTGGGG GCCCTCTTCT GTGTGCTGGG 
GTGGCCCAGG GCATCGTATC CTATGGACGG TCGGATGCAA AGCCCCCTGC TGTCTTCACC 
CGAATCTCCC ATTACCGGCC CTGGATCAAC CAGATCCTGC AGGCAAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CMA1 ( NM_001308083.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001295012.1 Homo sapiens chymase 1 (CMA1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu56952
Clone ID Related Accession (Same CDS sequence)	NM_001308083.2 , NM_001308083.1
Accession Version	NM_001308083.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	chymase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC103974.1, DQ082729.1 and HY275608.1. On Apr 28, 2015 this sequence version replaced XM_011536394.1. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an exon in the 5' coding region, which results in use of a downstream start codon compared to variant 1. It encodes isoform 2, which has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC103974.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2147975 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTACTAA AGTTGAAGGA GAAAGCCAGC CTGACCCTGG CTGTGGGGAC ACTCCCCTTC 
CCATCCCAAT TCAACTTTGT CCCACCTGGG AGAATGTGCC GGGTGGCTGG CTGGGGAAGA 
ACAGGTGTGT TGAAGCCGGG CTCAGACACT CTGCAAGAGG TGAAGCTGAG ACTCATGGAT 
CCCCAGGCCT GCAGCCACTT CAGAGACTTT GACCACAATC TTCAGCTGTG TGTGGGCAAT 
CCCAGGAAGA CAAAATCTGC ATTTAAGGGA GACTCTGGGG GCCCTCTTCT GTGTGCTGGG 
GTGGCCCAGG GCATCGTATC CTATGGACGG TCGGATGCAA AGCCCCCTGC TGTCTTCACC 
CGAATCTCCC ATTACCGGCC CTGGATCAAC CAGATCCTGC AGGCAAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295012.1
CDS	213..623
Translation	MLLKLKEKASLTLAVGTLPFPSQFNFVPPGRMCRVAGWGRTGVLKPGSDTLQEVKLRLMDPQACSHFRDFDHNLQLCVGNPRKTKSAFKGDSGGPLLCAGVAQGIVSYGRSDAKPPAVFTRISHYRPWINQILQAN

Target ORF information:

RefSeq Version	NM_001308083.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chymase 1 (CMA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308083.1

ORF Insert Sequence:

ATGTTACTAA AGTTGAAGGA GAAAGCCAGC CTGACCCTGG CTGTGGGGAC ACTCCCCTTC 
CCATCCCAAT TCAACTTTGT CCCACCTGGG AGAATGTGCC GGGTGGCTGG CTGGGGAAGA 
ACAGGTGTGT TGAAGCCGGG CTCAGACACT CTGCAAGAGG TGAAGCTGAG ACTCATGGAT 
CCCCAGGCCT GCAGCCACTT CAGAGACTTT GACCACAATC TTCAGCTGTG TGTGGGCAAT 
CCCAGGAAGA CAAAATCTGC ATTTAAGGGA GACTCTGGGG GCCCTCTTCT GTGTGCTGGG 
GTGGCCCAGG GCATCGTATC CTATGGACGG TCGGATGCAA AGCCCCCTGC TGTCTTCACC 
CGAATCTCCC ATTACCGGCC CTGGATCAAC CAGATCCTGC AGGCAAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Upregulation of cathepsin C expression contributes to endothelial chymase activation in preeclampsia.
Hypertension research : official journal of the Japanese Society of Hypertension40(12)976-981(2017 Dec)
Gu Y,Lewis DF,Alexander JS,Wang Y

Hypoxia-induced angiotensin II by the lactate-chymase-dependent mechanism mediates radioresistance of hypoxic tumor cells.
Scientific reports742396(2017 Feb)
Xie G,Liu Y,Yao Q,Zheng R,Zhang L,Lin J,Guo Z,Du S,Ren C,Yuan Q,Yuan Y

Highly Selective Cleavage of Cytokines and Chemokines by the Human Mast Cell Chymase and Neutrophil Cathepsin G.
Journal of immunology (Baltimore, Md. : 1950)198(4)1474-1483(2017 Feb)
Fu Z,Thorpe M,Alemayehu R,Roy A,Kervinen J,de Garavilla L,?brink M,Hellman L

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03-6811-6573
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CMA1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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オンラインフォーム

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