Cma1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cma1
Entrez Gene ID	17228
Full Name	chymase 1, mast cell
Synonyms	MMCP-5,Mcp-5,Mcp5,Mcpt5
General protein information	Preferred Names chymase 1, mast cell Names chymase alpha-chymase mast cell chymase 1 mast cell protease 5 mast cell protease I
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 C3\|14 28.19 cM
Summary	This gene encodes a serine protease that belongs to the peptidase family S1. It is expressed in mast cells and is thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. The encoded preproprotein undergoes proteolytic processing to generate a functional enzyme with elastase-like activity. Mice lacking the encoded protein exhibit significant attenuation of ischemia-reperfusion injury of the skeletal muscle. This gene is located in a cluster of related mast cell protease genes on chromosome 14. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_010780.3	NP_034910.2	chymase preproprotein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC540321	XP_002696732.1
Rattus norvegicus (Norway rat)	Cma1	NP_037224.1
Bos taurus (cattle)	LOC100139881	XP_002696686.1
Homo sapiens (human)	CMA1	NP_001827.1
Pan troglodytes (chimpanzee)	CMA1	XP_001170224.1
	CMA1	XP_001114321.1
Canis lupus familiaris (dog)	CMA1	NP_001013442.1
Mus musculus (house mouse)	Cma1	NP_034910.1

Gene Ontology
Bibliography

Related articles in PubMed

Experimental Arthritis Is Dependent on Mouse Mast Cell Protease-5.
Stevens RL, McNeil HP, Wensing LA, Shin K, Wong GW, Hansbro PM, Krilis SA
The Journal of biological chemistry292(13)5392-5404(2017 03)

Proteome analysis of mast cell releasates reveals a role for chymase in the regulation of coagulation factor XIIIA levels via proteolytic degradation.
Shubin NJ, Glukhova VA, Clauson M, Truong P, Abrink M, Pejler G, White NJ, Deutsch GH, Reeves SR, Vaisar T, James RG, Piliponsky AM
The Journal of allergy and clinical immunology139(1)323-334(2017 Jan)

The role of mouse mast cell proteases in the proliferative phase of wound healing in microdeformational wound therapy.
Succar J, Douaiher J, Lancerotto L, Li Q, Yamaguchi R, Younan G, Pejler G, Orgill DP
Plastic and reconstructive surgery134(3)459-67(2014 Sep)

Mouse mast cell proteases 4 and 5 mediate epidermal injury through disruption of tight junctions.
Bankova LG, Lezcano C, Pejler G, Stevens RL, Murphy GF, Austen KF, Gurish MF
Journal of immunology (Baltimore, Md. : 1950)192(6)2812-20(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Fibronectin was identified as a target of mMCP-5, and the exocytosis of mMCP-5 from the MCs in the mouse's peritoneal cavity resulted in the expression of metalloproteinase protease-9, which has been implicated in arthritis.
Title: Experimental Arthritis Is Dependent on Mouse Mast Cell Protease-5.

Mast cells have the ability to produce and degrade FXIIIA depending on their chymase expression profile: mast cells expressing chymase degrade FXIIIA, whereas mast cells that do not express chymase mainly produce FXIIIA
Title: Proteome analysis of mast cell releasates reveals a role for chymase in the regulation of coagulation factor XIIIA levels via proteolytic degradation.

Results suggest that mouse mast cell proteases 4, 5, and 6 are mediators of the critical role mast cells play in microdeformational wound therapy in the proliferative phase of healing.
Title: The role of mouse mast cell proteases in the proliferative phase of wound healing in microdeformational wound therapy.

Data indicate that a second-degree burn injury can initiate an immediate novel zonal degranulation of mast cell throughout all skin layers and a disruption of the epidermal tight junctions dependent on the nonredundant presence of mMCP4 and mMCP5.
Title: Mouse mast cell proteases 4 and 5 mediate epidermal injury through disruption of tight junctions.

mast cell/chymase-mediated intestinal epithelial barrier function is mediated by proteinase-activated receptor 2/MMP-2-dependent
Title: Chymase-mediated intestinal epithelial permeability is regulated by a protease-activating receptor/matrix metalloproteinase-2-dependent mechanism.

The following Cma1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cma1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu83646	NM_010780.3 Latest version!	Mus musculus chymase 1, mast cell (Cma1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cma1 ( NM_010780.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034910.2 Mus musculus chymase 1, mast cell (Cma1), mRNA.

Price & Availability↑

CloneID	OMu83646
Clone ID Related Accession (Same CDS sequence)	NM_010780.3
Accession Version	NM_010780.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	chymase preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC163646.2 and BC139457.1. On Nov 13, 2015 this sequence version replaced NM_010780.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X68805.1, M73759.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCATCTTC TTACTCTTCA TCTGCTGCTC CTTCTCCTGG GTTCCAGCAC CAAAGCTGGA 
GAGATCATTG GAGGCACGGA GTGCATACCA CACTCCCGCC CCTACATGGC CTATCTGGAA 
ATTGTCACTT CTGAGAACTA CCTGTCGGCC TGCAGTGGCT TCCTGATAAG AAGAAACTTT 
GTGCTGACTG CAGCTCACTG TGCGGGAAGG TCTATAACAG TCCTCCTAGG AGCCCATAAC 
AAAACATCTA AAGAAGACAC GTGGCAGAAG CTTGAGGTGG AAAAGCAATT CCTTCATCCA 
AAATATGATG AGAATTTGGT TGTCCACGAC ATCATGCTAC TGAAGTTGAA GGAGAAAGCC 
AAGCTAACCC TAGGTGTGGG AACCCTCCCA CTCTCTGCCA ACTTCAACTT TATCCCACCC 
GGGAGAATGT GCAGGGCAGT TGGCTGGGGC AGAACAAACG TGAATGAGCC AGCCTCCGAC 
ACACTGCAGG AAGTAAAGAT GAGACTCCAG GAGCCCCAAG CCTGCAAACA CTTCACCAGT 
TTTCGACACA ATTCCCAGCT GTGTGTGGGC AACCCCAAGA AGATGCAAAA TGTATACAAG 
GGAGACTCTG GAGGACCTCT GCTGTGTGCT GGGATAGCCC AAGGCATTGC ATCCTATGTA 
CATCGGAATG CAAAGCCCCC TGCTGTCTTT ACCAGAATCT CCCATTACAG GCCCTGGATC 
AATAAGATCT TGAGGGAGAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034910.2
CDS	144..887
Translation	MHLLTLHLLLLLLGSSTKAGEIIGGTECIPHSRPYMAYLEIVTSENYLSACSGFLIRRNFVLTAAHCAGRSITVLLGAHNKTSKEDTWQKLEVEKQFLHPKYDENLVVHDIMLLKLKEKAKLTLGVGTLPLSANFNFIPPGRMCRAVGWGRTNVNEPASDTLQEVKMRLQEPQACKHFTSFRHNSQLCVGNPKKMQNVYKGDSGGPLLCAGIAQGIASYVHRNAKPPAVFTRISHYRPWINKILREN

Target ORF information:

RefSeq Version	NM_010780.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus chymase 1, mast cell (Cma1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010780.3

ORF Insert Sequence:

ATGCATCTTC TTACTCTTCA TCTGCTGCTC CTTCTCCTGG GTTCCAGCAC CAAAGCTGGA 
GAGATCATTG GAGGCACGGA GTGCATACCA CACTCCCGCC CCTACATGGC CTATCTGGAA 
ATTGTCACTT CTGAGAACTA CCTGTCGGCC TGCAGTGGCT TCCTGATAAG AAGAAACTTT 
GTGCTGACTG CAGCTCACTG TGCGGGAAGG TCTATAACAG TCCTCCTAGG AGCCCATAAC 
AAAACATCTA AAGAAGACAC GTGGCAGAAG CTTGAGGTGG AAAAGCAATT CCTTCATCCA 
AAATATGATG AGAATTTGGT TGTCCACGAC ATCATGCTAC TGAAGTTGAA GGAGAAAGCC 
AAGCTAACCC TAGGTGTGGG AACCCTCCCA CTCTCTGCCA ACTTCAACTT TATCCCACCC 
GGGAGAATGT GCAGGGCAGT TGGCTGGGGC AGAACAAACG TGAATGAGCC AGCCTCCGAC 
ACACTGCAGG AAGTAAAGAT GAGACTCCAG GAGCCCCAAG CCTGCAAACA CTTCACCAGT 
TTTCGACACA ATTCCCAGCT GTGTGTGGGC AACCCCAAGA AGATGCAAAA TGTATACAAG 
GGAGACTCTG GAGGACCTCT GCTGTGTGCT GGGATAGCCC AAGGCATTGC ATCCTATGTA 
CATCGGAATG CAAAGCCCCC TGCTGTCTTT ACCAGAATCT CCCATTACAG GCCCTGGATC 
AATAAGATCT TGAGGGAGAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Experimental Arthritis Is Dependent on Mouse Mast Cell Protease-5.
The Journal of biological chemistry292(13)5392-5404(2017 03)
Stevens RL,McNeil HP,Wensing LA,Shin K,Wong GW,Hansbro PM,Krilis SA

Proteome analysis of mast cell releasates reveals a role for chymase in the regulation of coagulation factor XIIIA levels via proteolytic degradation.
The Journal of allergy and clinical immunology139(1)323-334(2017 Jan)
Shubin NJ,Glukhova VA,Clauson M,Truong P,Abrink M,Pejler G,White NJ,Deutsch GH,Reeves SR,Vaisar T,James RG,Piliponsky AM

The role of mouse mast cell proteases in the proliferative phase of wound healing in microdeformational wound therapy.
Plastic and reconstructive surgery134(3)459-67(2014 Sep)
Succar J,Douaiher J,Lancerotto L,Li Q,Yamaguchi R,Younan G,Pejler G,Orgill DP

Mouse mast cell proteases 4 and 5 mediate epidermal injury through disruption of tight junctions.
Journal of immunology (Baltimore, Md. : 1950)192(6)2812-20(2014 Mar)
Bankova LG,Lezcano C,Pejler G,Stevens RL,Murphy GF,Austen KF,Gurish MF

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Cma1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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