BUD23 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BUD23
Entrez Gene ID	114049
Full Name	BUD23, rRNA methyltransferase and ribosome maturation factor
Synonyms	HASJ4442,HUSSY-3,MERM1,PP3381,WBMT,WBSCR22
General protein information	Preferred Names BUD23, rRNA methyltransferase and ribosome maturation factor Names probable 18S rRNA (guanine-N(7))-methyltransferase Williams-Beuren candidate region putative methyltransferase Williams-Beuren syndrome chromosomal region 22 protein Williams-Beuren syndrome chromosome region 22 bud site selection protein 23 homolog metastasis-related methyltransferase 1 rRNA methyltransferase and ribosome maturation factor ribosome biogenesis methyltransferase WBSCR22
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q11.23
Summary	This gene encodes a protein containing a nuclear localization signal and an S-adenosyl-L-methionine binding motif typical of methyltransferases, suggesting that the encoded protein may act on DNA methylation. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternatively spliced transcript variants have been found. [provided by RefSeq, Feb 2011].
Disorder MIM:	615733

mRNA and Protein(s)

mRNA	Protein	Name
XM_006715847.1	XP_006715910.1	probable 18S rRNA (guanine-N(7))-methyltransferase isoform X1
NM_001202560.2	NP_001189489.1	probable 18S rRNA (guanine-N(7))-methyltransferase isoform 1
XM_011515779.1	XP_011514081.1	probable 18S rRNA (guanine-N(7))-methyltransferase isoform X3
NM_017528.4	NP_059998.2	probable 18S rRNA (guanine-N(7))-methyltransferase isoform 2
NM_017528.5	NP_059998.2	probable 18S rRNA (guanine-N(7))-methyltransferase isoform 2
XM_011515778.1	XP_011514080.1	probable 18S rRNA (guanine-N(7))-methyltransferase isoform X2
XM_011515779.2	XP_011514081.1	probable 18S rRNA (guanine-N(7))-methyltransferase isoform X3
NM_001202560.3	NP_001189489.1	probable 18S rRNA (guanine-N(7))-methyltransferase isoform 1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG10903	NP_649762.1
Schizosaccharomyces pombe (fission yeast)	SPAC26A3.06	NP_594149.1
Neurospora crassa	NCU00777	XP_964933.1
Arabidopsis thaliana (thale cress)	AT5G57280	NP_200538.1
Homo sapiens (human)	WBSCR22	NP_001189489.1
Bos taurus (cattle)	WBSCR22	NP_001029629.2
Mus musculus (house mouse)	Wbscr22	NP_079651.2
Caenorhabditis elegans (roundworm)	C27F2.4	NP_498051.1
Eremothecium gossypii	AGOS_AAR066W	NP_982607.1
Oryza sativa (rice)	Os02g0804300	NP_001048432.1
Pan troglodytes (chimpanzee)	WBSCR22	XP_003318696.1
	WBSCR22	XP_001115175.2
Gallus gallus (chicken)	WBSCR22	NP_001034421.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003728	XP_310259.2
Kluyveromyces lactis	KLLA0F05962g	XP_455350.1
Magnaporthe oryzae (rice blast fungus)	MGG_08705	XP_003719275.1
Canis lupus familiaris (dog)	WBSCR22	XP_857405.2
Xenopus tropicalis (tropical clawed frog)	wbscr22	NP_001011475.1
Rattus norvegicus (Norway rat)	Wbscr22	NP_001129215.1
Danio rerio (zebrafish)	wbscr22	NP_001076348.1
Saccharomyces cerevisiae (baker's yeast)	BUD23	NP_009976.1

Gene Ontology
Bibliography

Related articles in PubMed

WBSCR22 confers oxaliplatin resistance in human colorectal cancer.
Yan D, Tu L, Yuan H, Fang J, Cheng L, Zheng X, Wang X
Scientific reports7(1)15443(2017 Nov)

The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis.
Zorbas C, Nicolas E, Wacheul L, Huvelle E, Heurgu?-Hamard V, Lafontaine DL
Molecular biology of the cell26(11)2080-95(2015 Jun)

WBSCR22/Merm1 is required for late nuclear pre-ribosomal RNA processing and mediates N7-methylation of G1639 in human 18S rRNA.
Haag S, Kretschmer J, Bohnsack MT
RNA (New York, N.Y.)21(2)180-7(2015 Feb)

Knockdown of Merm1/Wbscr22 attenuates sensitivity of H460 non-small cell lung cancer cells to SN-38 and 5-FU without alteration to p53 expression levels.
Yan D, Zheng X, Tu L, Jia J, Li Q, Cheng L, Wang X
Molecular medicine reports11(1)295-302(2015 Jan)

The Stability of Ribosome Biogenesis Factor WBSCR22 Is Regulated by Interaction with TRMT112 via Ubiquitin-Proteasome Pathway.
?unap K, Leetsi L, Matsoo M, Kurg R
PloS one10(7)e0133841(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DIMT1L and WBSCR22-TRMT112 are required for distinct pre-rRNA processing reactions leading to synthesis of 18S rRNA. Ribosome biogenesis requires the presence of the modification enzyme rather than its RNA-modifying catalytic activity.
Title: The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis.

The key role of WBSCR22 in 40S subunit biogenesis is independent of its function as an RNA methyltransferase.
Title: WBSCR22/Merm1 is required for late nuclear pre-ribosomal RNA processing and mediates N7-methylation of G1639 in human 18S rRNA.

Knockdown of Merm1/Wbscr22 attenuates sensitivity of H460 non-small cell lung cancer cells to SN-38 and 5-FU without alteration to p53 expression levels.
Title: Knockdown of Merm1/Wbscr22 attenuates sensitivity of H460 non-small cell lung cancer cells to SN-38 and 5-FU without alteration to p53 expression levels.

The localization of the TRMT112 protein is determined by WBSCR22, and the WBSCR22-TRMT112 complex is localized in the cell nucleus.
Title: The Stability of Ribosome Biogenesis Factor WBSCR22 Is Regulated by Interaction with TRMT112 via Ubiquitin-Proteasome Pathway.

Merm1 is a novel regulator of chromatin structure affecting GR recruitment and function
Title: The methyltransferase WBSCR22/Merm1 enhances glucocorticoid receptor function and is regulated in lung inflammation and cancer.

The following BUD23 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUD23 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26908	NM_017528.5 Latest version!	Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu27350	NM_001202560.3 Latest version!	Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu41353	XM_006715847.1 Latest version!	Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu74728	XM_011515778.1 Latest version!	Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu74729	XM_011515779.2 Latest version!	Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu26908	NM_017528.4	Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu74729	XM_011515779.1	Homo sapiens Williams-Beuren syndrome chromosome region 22 (WBSCR22), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu27350	NM_001202560.2	Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BUD23 ( NM_017528.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_059998.2 Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26908
Clone ID Related Accession (Same CDS sequence)	NM_017528.4 , NM_017528.5
Accession Version	NM_017528.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	probable 18S rRNA (guanine-N(7))-methyltransferase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF412034.1, AA508840.1 and BM671506.1. On Nov 11, 2011 this sequence version replaced NM_017528.3. Transcript Variant: This variant (2) lacks an exon in the 3' coding region but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.535606.1, SRR1163655.85310.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCCC GCGGCCGGCG TCCGGAGCAT GGCGGACCCC CAGAGCTGTT TTATGACGAG 
ACAGAAGCCC GGAAATACGT TCGCAACTCA CGGATGATTG ATATCCAGAC CAGGATGGCT 
GGGCGAGCAT TGGAGCTTCT TTATCTGCCA GAGAATAAGC CCTGTTACCT GCTGGATATT 
GGCTGTGGCA CTGGGCTGAG TGGAAGTTAT CTGTCAGATG AAGGGCACTA TTGGGTGGGC 
CTGGATATCA GCCCTGCCAT GCTGGATGAG GCTGTGGACC GAGAGATAGA GGGAGACCTG 
CTGCTGGGGG ATATGGGCCA GGGCATCCCA TTCAAGCCAG GCACATTTGA TGGTTGCATC 
AGCATTTCTG CTGTGCAGTG GCTCTGTAAT GCTAACAAGA AGTCTGAAAA CCCTGCCAAG 
CGCCTGTACT GCTTTTTTGC TTCTCTTTTT TCTGTTCTCG TCCGGGGATC CCGAGCTGTC 
CTGCAGCTGT ACCCTGAGAA CTCAGAGCAG TTGGAGCTGA TCACAACCCA GGCCACAAAG 
GCAGGCTTCT CCGGTGGCAT GGTGGTAGAC TACCCTAACA GTGCCAAAGC AAAGAAATTC 
TACCTCTGCT TGTTTTCTGG GCCTTCGACC TTTATACCAG AGGGGCTGAG TGAAAATCAG 
GATGAAGTTG AACCCAGGGA GTCTGTGTTC ACCAATGAGA GGTTCCCATT AAGGATGTCG 
AGGCGGGGAA TGGTGAGGAA GAGTCGGGCA TGGGTGCTGG AGAAGAAGGA GCGGCACAGG 
CGCCAGGGCA GGGAAGTCAG ACCTGACACC CAGTACACCG GCCGCAAGCG CAAGCCCCGC 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059998.2
CDS	59..904
Translation	MASRGRRPEHGGPPELFYDETEARKYVRNSRMIDIQTRMAGRALELLYLPENKPCYLLDIGCGTGLSGSYLSDEGHYWVGLDISPAMLDEAVDREIEGDLLLGDMGQGIPFKPGTFDGCISISAVQWLCNANKKSENPAKRLYCFFASLFSVLVRGSRAVLQLYPENSEQLELITTQATKAGFSGGMVVDYPNSAKAKKFYLCLFSGPSTFIPEGLSENQDEVEPRESVFTNERFPLRMSRRGMVRKSRAWVLEKKERHRRQGREVRPDTQYTGRKRKPRF

Target ORF information:

RefSeq Version	NM_017528.4
Organism	Homo sapiens(human)
Definition	Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017528.4

ORF Insert Sequence:

ATGGCGTCCC GCGGCCGGCG TCCGGAGCAT GGCGGACCCC CAGAGCTGTT TTATGACGAG 
ACAGAAGCCC GGAAATACGT TCGCAACTCA CGGATGATTG ATATCCAGAC CAGGATGGCT 
GGGCGAGCAT TGGAGCTTCT TTATCTGCCA GAGAATAAGC CCTGTTACCT GCTGGATATT 
GGCTGTGGCA CTGGGCTGAG TGGAAGTTAT CTGTCAGATG AAGGGCACTA TTGGGTGGGC 
CTGGATATCA GCCCTGCCAT GCTGGATGAG GCTGTGGACC GAGAGATAGA GGGAGACCTG 
CTGCTGGGGG ATATGGGCCA GGGCATCCCA TTCAAGCCAG GCACATTTGA TGGTTGCATC 
AGCATTTCTG CTGTGCAGTG GCTCTGTAAT GCTAACAAGA AGTCTGAAAA CCCTGCCAAG 
CGCCTGTACT GCTTTTTTGC TTCTCTTTTT TCTGTTCTCG TCCGGGGATC CCGAGCTGTC 
CTGCAGCTGT ACCCTGAGAA CTCAGAGCAG TTGGAGCTGA TCACAACCCA GGCCACAAAG 
GCAGGCTTCT CCGGTGGCAT GGTGGTAGAC TACCCTAACA GTGCCAAAGC AAAGAAATTC 
TACCTCTGCT TGTTTTCTGG GCCTTCGACC TTTATACCAG AGGGGCTGAG TGAAAATCAG 
GATGAAGTTG AACCCAGGGA GTCTGTGTTC ACCAATGAGA GGTTCCCATT AAGGATGTCG 
AGGCGGGGAA TGGTGAGGAA GAGTCGGGCA TGGGTGCTGG AGAAGAAGGA GCGGCACAGG 
CGCCAGGGCA GGGAAGTCAG ACCTGACACC CAGTACACCG GCCGCAAGCG CAAGCCCCGC 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUD23 ( NM_017528.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_059998.2 Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26908
Clone ID Related Accession (Same CDS sequence)	NM_017528.4 , NM_017528.5
Accession Version	NM_017528.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	probable 18S rRNA (guanine-N(7))-methyltransferase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF412034.1 and AA508840.1. On Jun 2, 2019 this sequence version replaced NM_017528.4. Transcript Variant: This variant (2) lacks an exon in the 3' coding region but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DRR138512.636920.1, BC011696.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCCC GCGGCCGGCG TCCGGAGCAT GGCGGACCCC CAGAGCTGTT TTATGACGAG 
ACAGAAGCCC GGAAATACGT TCGCAACTCA CGGATGATTG ATATCCAGAC CAGGATGGCT 
GGGCGAGCAT TGGAGCTTCT TTATCTGCCA GAGAATAAGC CCTGTTACCT GCTGGATATT 
GGCTGTGGCA CTGGGCTGAG TGGAAGTTAT CTGTCAGATG AAGGGCACTA TTGGGTGGGC 
CTGGATATCA GCCCTGCCAT GCTGGATGAG GCTGTGGACC GAGAGATAGA GGGAGACCTG 
CTGCTGGGGG ATATGGGCCA GGGCATCCCA TTCAAGCCAG GCACATTTGA TGGTTGCATC 
AGCATTTCTG CTGTGCAGTG GCTCTGTAAT GCTAACAAGA AGTCTGAAAA CCCTGCCAAG 
CGCCTGTACT GCTTTTTTGC TTCTCTTTTT TCTGTTCTCG TCCGGGGATC CCGAGCTGTC 
CTGCAGCTGT ACCCTGAGAA CTCAGAGCAG TTGGAGCTGA TCACAACCCA GGCCACAAAG 
GCAGGCTTCT CCGGTGGCAT GGTGGTAGAC TACCCTAACA GTGCCAAAGC AAAGAAATTC 
TACCTCTGCT TGTTTTCTGG GCCTTCGACC TTTATACCAG AGGGGCTGAG TGAAAATCAG 
GATGAAGTTG AACCCAGGGA GTCTGTGTTC ACCAATGAGA GGTTCCCATT AAGGATGTCG 
AGGCGGGGAA TGGTGAGGAA GAGTCGGGCA TGGGTGCTGG AGAAGAAGGA GCGGCACAGG 
CGCCAGGGCA GGGAAGTCAG ACCTGACACC CAGTACACCG GCCGCAAGCG CAAGCCCCGC 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059998.2
CDS	30..875
Translation	MASRGRRPEHGGPPELFYDETEARKYVRNSRMIDIQTRMAGRALELLYLPENKPCYLLDIGCGTGLSGSYLSDEGHYWVGLDISPAMLDEAVDREIEGDLLLGDMGQGIPFKPGTFDGCISISAVQWLCNANKKSENPAKRLYCFFASLFSVLVRGSRAVLQLYPENSEQLELITTQATKAGFSGGMVVDYPNSAKAKKFYLCLFSGPSTFIPEGLSENQDEVEPRESVFTNERFPLRMSRRGMVRKSRAWVLEKKERHRRQGREVRPDTQYTGRKRKPRF

Target ORF information:

RefSeq Version	NM_017528.5
Organism	Homo sapiens(human)
Definition	Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017528.5

ORF Insert Sequence:

ATGGCGTCCC GCGGCCGGCG TCCGGAGCAT GGCGGACCCC CAGAGCTGTT TTATGACGAG 
ACAGAAGCCC GGAAATACGT TCGCAACTCA CGGATGATTG ATATCCAGAC CAGGATGGCT 
GGGCGAGCAT TGGAGCTTCT TTATCTGCCA GAGAATAAGC CCTGTTACCT GCTGGATATT 
GGCTGTGGCA CTGGGCTGAG TGGAAGTTAT CTGTCAGATG AAGGGCACTA TTGGGTGGGC 
CTGGATATCA GCCCTGCCAT GCTGGATGAG GCTGTGGACC GAGAGATAGA GGGAGACCTG 
CTGCTGGGGG ATATGGGCCA GGGCATCCCA TTCAAGCCAG GCACATTTGA TGGTTGCATC 
AGCATTTCTG CTGTGCAGTG GCTCTGTAAT GCTAACAAGA AGTCTGAAAA CCCTGCCAAG 
CGCCTGTACT GCTTTTTTGC TTCTCTTTTT TCTGTTCTCG TCCGGGGATC CCGAGCTGTC 
CTGCAGCTGT ACCCTGAGAA CTCAGAGCAG TTGGAGCTGA TCACAACCCA GGCCACAAAG 
GCAGGCTTCT CCGGTGGCAT GGTGGTAGAC TACCCTAACA GTGCCAAAGC AAAGAAATTC 
TACCTCTGCT TGTTTTCTGG GCCTTCGACC TTTATACCAG AGGGGCTGAG TGAAAATCAG 
GATGAAGTTG AACCCAGGGA GTCTGTGTTC ACCAATGAGA GGTTCCCATT AAGGATGTCG 
AGGCGGGGAA TGGTGAGGAA GAGTCGGGCA TGGGTGCTGG AGAAGAAGGA GCGGCACAGG 
CGCCAGGGCA GGGAAGTCAG ACCTGACACC CAGTACACCG GCCGCAAGCG CAAGCCCCGC 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUD23 ( NM_001202560.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001189489.1 Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27350
Clone ID Related Accession (Same CDS sequence)	NM_001202560.2 , NM_001202560.3
Accession Version	NM_001202560.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-20
Organism	Homo sapiens(human)
Product	probable 18S rRNA (guanine-N(7))-methyltransferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF412034.1, BQ927478.1, AA099700.1, AA508840.1 and BM671506.1. On Nov 11, 2011 this sequence version replaced NM_001202560.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.257967.1, BQ927478.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCCC GCGGCCGGCG TCCGGAGCAT GGCGGACCCC CAGAGCTGTT TTATGACGAG 
ACAGAAGCCC GGAAATACGT TCGCAACTCA CGGATGATTG ATATCCAGAC CAGGATGGCT 
GGGCGAGCAT TGGAGCTTCT TTATCTGCCA GAGAATAAGC CCTGTTACCT GCTGGATATT 
GGCTGTGGCA CTGGGCTGAG TGGAAGTTAT CTGTCAGATG AAGGGCACTA TTGGGTGGGC 
CTGGATATCA GCCCTGCCAT GCTGGATGAG GCTGTGGACC GAGAGATAGA GGGAGACCTG 
CTGCTGGGGG ATATGGGCCA GGGCATCCCA TTCAAGCCAG GCACATTTGA TGGTTGCATC 
AGCATTTCTG CTGTGCAGTG GCTCTGTAAT GCTAACAAGA AGTCTGAAAA CCCTGCCAAG 
CGCCTGTACT GCTTTTTTGC TTCTCTTTTT TCTGTTCTCG TCCGGGGATC CCGAGCTGTC 
CTGCAGCTGT ACCCTGAGAA CTCAGAGCAG TTGGAGCTGA TCACAACCCA GGCCACAAAG 
GCAGGCTTCT CCGGTGGCAT GGTGGTAGAC TACCCTAACA GTGCCAAAGC AAAGAAATTC 
TACCTCTGCT TGTTTTCTGG GCCTTCGACC TTTATACCAG AGGGGCTGAG TGAAAATCAG 
GATGAAGTTG AACCCAGGGA GTCTGTGTTC ACCAATGAGA GGGAAGGTGG AGCATTTGAG 
AGAAGGGGCA TCCGAGGCCA CCAGACTCGG AGGTTCCCAT TAAGGATGTC GAGGCGGGGA 
ATGGTGAGGA AGAGTCGGGC ATGGGTGCTG GAGAAGAAGG AGCGGCACAG GCGCCAGGGC 
AGGGAAGTCA GACCTGACAC CCAGTACACC GGCCGCAAGC GCAAGCCCCG CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189489.1
CDS	59..955
Translation	MASRGRRPEHGGPPELFYDETEARKYVRNSRMIDIQTRMAGRALELLYLPENKPCYLLDIGCGTGLSGSYLSDEGHYWVGLDISPAMLDEAVDREIEGDLLLGDMGQGIPFKPGTFDGCISISAVQWLCNANKKSENPAKRLYCFFASLFSVLVRGSRAVLQLYPENSEQLELITTQATKAGFSGGMVVDYPNSAKAKKFYLCLFSGPSTFIPEGLSENQDEVEPRESVFTNEREGGAFERRGIRGHQTRRFPLRMSRRGMVRKSRAWVLEKKERHRRQGREVRPDTQYTGRKRKPRF

Target ORF information:

RefSeq Version	NM_001202560.2
Organism	Homo sapiens(human)
Definition	Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202560.2

ORF Insert Sequence:

ATGGCGTCCC GCGGCCGGCG TCCGGAGCAT GGCGGACCCC CAGAGCTGTT TTATGACGAG 
ACAGAAGCCC GGAAATACGT TCGCAACTCA CGGATGATTG ATATCCAGAC CAGGATGGCT 
GGGCGAGCAT TGGAGCTTCT TTATCTGCCA GAGAATAAGC CCTGTTACCT GCTGGATATT 
GGCTGTGGCA CTGGGCTGAG TGGAAGTTAT CTGTCAGATG AAGGGCACTA TTGGGTGGGC 
CTGGATATCA GCCCTGCCAT GCTGGATGAG GCTGTGGACC GAGAGATAGA GGGAGACCTG 
CTGCTGGGGG ATATGGGCCA GGGCATCCCA TTCAAGCCAG GCACATTTGA TGGTTGCATC 
AGCATTTCTG CTGTGCAGTG GCTCTGTAAT GCTAACAAGA AGTCTGAAAA CCCTGCCAAG 
CGCCTGTACT GCTTTTTTGC TTCTCTTTTT TCTGTTCTCG TCCGGGGATC CCGAGCTGTC 
CTGCAGCTGT ACCCTGAGAA CTCAGAGCAG TTGGAGCTGA TCACAACCCA GGCCACAAAG 
GCAGGCTTCT CCGGTGGCAT GGTGGTAGAC TACCCTAACA GTGCCAAAGC AAAGAAATTC 
TACCTCTGCT TGTTTTCTGG GCCTTCGACC TTTATACCAG AGGGGCTGAG TGAAAATCAG 
GATGAAGTTG AACCCAGGGA GTCTGTGTTC ACCAATGAGA GGGAAGGTGG AGCATTTGAG 
AGAAGGGGCA TCCGAGGCCA CCAGACTCGG AGGTTCCCAT TAAGGATGTC GAGGCGGGGA 
ATGGTGAGGA AGAGTCGGGC ATGGGTGCTG GAGAAGAAGG AGCGGCACAG GCGCCAGGGC 
AGGGAAGTCA GACCTGACAC CCAGTACACC GGCCGCAAGC GCAAGCCCCG CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUD23 ( NM_001202560.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001189489.1 Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant 1, mRNA.

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CloneID	OHu27350
Clone ID Related Accession (Same CDS sequence)	NM_001202560.2 , NM_001202560.3
Accession Version	NM_001202560.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-14
Organism	Homo sapiens(human)
Product	probable 18S rRNA (guanine-N(7))-methyltransferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF412034.1, BQ927478.1, AA099700.1 and AA508840.1. On Jun 2, 2019 this sequence version replaced NM_001202560.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.195882.1, SRR1163655.257967.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCCC GCGGCCGGCG TCCGGAGCAT GGCGGACCCC CAGAGCTGTT TTATGACGAG 
ACAGAAGCCC GGAAATACGT TCGCAACTCA CGGATGATTG ATATCCAGAC CAGGATGGCT 
GGGCGAGCAT TGGAGCTTCT TTATCTGCCA GAGAATAAGC CCTGTTACCT GCTGGATATT 
GGCTGTGGCA CTGGGCTGAG TGGAAGTTAT CTGTCAGATG AAGGGCACTA TTGGGTGGGC 
CTGGATATCA GCCCTGCCAT GCTGGATGAG GCTGTGGACC GAGAGATAGA GGGAGACCTG 
CTGCTGGGGG ATATGGGCCA GGGCATCCCA TTCAAGCCAG GCACATTTGA TGGTTGCATC 
AGCATTTCTG CTGTGCAGTG GCTCTGTAAT GCTAACAAGA AGTCTGAAAA CCCTGCCAAG 
CGCCTGTACT GCTTTTTTGC TTCTCTTTTT TCTGTTCTCG TCCGGGGATC CCGAGCTGTC 
CTGCAGCTGT ACCCTGAGAA CTCAGAGCAG TTGGAGCTGA TCACAACCCA GGCCACAAAG 
GCAGGCTTCT CCGGTGGCAT GGTGGTAGAC TACCCTAACA GTGCCAAAGC AAAGAAATTC 
TACCTCTGCT TGTTTTCTGG GCCTTCGACC TTTATACCAG AGGGGCTGAG TGAAAATCAG 
GATGAAGTTG AACCCAGGGA GTCTGTGTTC ACCAATGAGA GGGAAGGTGG AGCATTTGAG 
AGAAGGGGCA TCCGAGGCCA CCAGACTCGG AGGTTCCCAT TAAGGATGTC GAGGCGGGGA 
ATGGTGAGGA AGAGTCGGGC ATGGGTGCTG GAGAAGAAGG AGCGGCACAG GCGCCAGGGC 
AGGGAAGTCA GACCTGACAC CCAGTACACC GGCCGCAAGC GCAAGCCCCG CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189489.1
CDS	30..926
Translation	MASRGRRPEHGGPPELFYDETEARKYVRNSRMIDIQTRMAGRALELLYLPENKPCYLLDIGCGTGLSGSYLSDEGHYWVGLDISPAMLDEAVDREIEGDLLLGDMGQGIPFKPGTFDGCISISAVQWLCNANKKSENPAKRLYCFFASLFSVLVRGSRAVLQLYPENSEQLELITTQATKAGFSGGMVVDYPNSAKAKKFYLCLFSGPSTFIPEGLSENQDEVEPRESVFTNEREGGAFERRGIRGHQTRRFPLRMSRRGMVRKSRAWVLEKKERHRRQGREVRPDTQYTGRKRKPRF

Target ORF information:

RefSeq Version	NM_001202560.3
Organism	Homo sapiens(human)
Definition	Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202560.3

ORF Insert Sequence:

ATGGCGTCCC GCGGCCGGCG TCCGGAGCAT GGCGGACCCC CAGAGCTGTT TTATGACGAG 
ACAGAAGCCC GGAAATACGT TCGCAACTCA CGGATGATTG ATATCCAGAC CAGGATGGCT 
GGGCGAGCAT TGGAGCTTCT TTATCTGCCA GAGAATAAGC CCTGTTACCT GCTGGATATT 
GGCTGTGGCA CTGGGCTGAG TGGAAGTTAT CTGTCAGATG AAGGGCACTA TTGGGTGGGC 
CTGGATATCA GCCCTGCCAT GCTGGATGAG GCTGTGGACC GAGAGATAGA GGGAGACCTG 
CTGCTGGGGG ATATGGGCCA GGGCATCCCA TTCAAGCCAG GCACATTTGA TGGTTGCATC 
AGCATTTCTG CTGTGCAGTG GCTCTGTAAT GCTAACAAGA AGTCTGAAAA CCCTGCCAAG 
CGCCTGTACT GCTTTTTTGC TTCTCTTTTT TCTGTTCTCG TCCGGGGATC CCGAGCTGTC 
CTGCAGCTGT ACCCTGAGAA CTCAGAGCAG TTGGAGCTGA TCACAACCCA GGCCACAAAG 
GCAGGCTTCT CCGGTGGCAT GGTGGTAGAC TACCCTAACA GTGCCAAAGC AAAGAAATTC 
TACCTCTGCT TGTTTTCTGG GCCTTCGACC TTTATACCAG AGGGGCTGAG TGAAAATCAG 
GATGAAGTTG AACCCAGGGA GTCTGTGTTC ACCAATGAGA GGGAAGGTGG AGCATTTGAG 
AGAAGGGGCA TCCGAGGCCA CCAGACTCGG AGGTTCCCAT TAAGGATGTC GAGGCGGGGA 
ATGGTGAGGA AGAGTCGGGC ATGGGTGCTG GAGAAGAAGG AGCGGCACAG GCGCCAGGGC 
AGGGAAGTCA GACCTGACAC CCAGTACACC GGCCGCAAGC GCAAGCCCCG CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUD23 ( XM_006715847.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006715910.1 Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant X2, mRNA.

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CloneID	OHu41353
Clone ID Related Accession (Same CDS sequence)	XM_006715847.1
Accession Version	XM_006715847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable 18S rRNA (guanine-N(7))-methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACC CCCAGAGCTG TTTTATGACG AGACAGAAGC CCGGAAATAC GTTCGCAAGT 
GAGGGGAGCC TGAATACTGC GGGGCGTCCG GGGGTCGGGA AGCGGCAAGT TGCCCCTGTT 
GCTGGCGTTG CCCGGAAAGG CCGTAGAATT TGGGGCTCAC GGATGATTGA TATCCAGACC 
AGGATGGCTG GGCGAGCATT GGAGCTTCTT TATCTGCCAG AGAATAAGCC CTGTTACCTG 
CTGGATATTG GCTGTGGCAC TGGGCTGAGT GGAAGTTATC TGTCAGATGA AGGGCACTAT 
TGGGTGGGCC TGGATATCAG CCCTGCCATG CTGGATGAGG CTGTGGACCG AGAGATAGAG 
GGAGACCTGC TGCTGGGGGA TATGGGCCAG GGCATCCCAT TCAAGCCAGG CACATTTGAT 
GGTTGCATCA GCATTTCTGC TGTGCAGTGG CTCTGTAATG CTAACAAGAA GTCTGAAAAC 
CCTGCCAAGC GCCTGTACTG CTTTTTTGCT TCTCTTTTTT CTGTTCTCGT CCGGGGATCC 
CGAGCTGTCC TGCAGCTGTA CCCTGAGAAC TCAGAGCAGT TGGAGCTGAT CACAACCCAG 
GCCACAAAGG CAGGCTTCTC CGGTGGCATG GTGGTAGACT ACCCTAACAG TGCCAAAGCA 
AAGAAATTCT ACCTCTGCTT GTTTTCTGGG CCTTCGACCT TTATACCAGA GGGGCTGAGT 
GAAAATCAGG ATGAAGTTGA ACCCAGGGAG TCTGTGTTCA CCAATGAGAG GTTCCCATTA 
AGGATGTCGA GGCGGGGAAT GGTGAGGAAG AGTCGGGCAT GGGTGCTGGA GAAGAAGGAG 
CGGCACAGGC GCCAGGGCAG GGAAGTCAGA CCTGACACCC AGTACACCGG CCGCAAGCGC 
AAGCCCCGCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006715910.1
CDS	83..997
Translation	MADPQSCFMTRQKPGNTFASEGSLNTAGRPGVGKRQVAPVAGVARKGRRIWGSRMIDIQTRMAGRALELLYLPENKPCYLLDIGCGTGLSGSYLSDEGHYWVGLDISPAMLDEAVDREIEGDLLLGDMGQGIPFKPGTFDGCISISAVQWLCNANKKSENPAKRLYCFFASLFSVLVRGSRAVLQLYPENSEQLELITTQATKAGFSGGMVVDYPNSAKAKKFYLCLFSGPSTFIPEGLSENQDEVEPRESVFTNERFPLRMSRRGMVRKSRAWVLEKKERHRRQGREVRPDTQYTGRKRKPRF

Target ORF information:

RefSeq Version	XM_006715847.1
Organism	Homo sapiens(human)
Definition	Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006715847.1

ORF Insert Sequence:

ATGGCGGACC CCCAGAGCTG TTTTATGACG AGACAGAAGC CCGGAAATAC GTTCGCAAGT 
GAGGGGAGCC TGAATACTGC GGGGCGTCCG GGGGTCGGGA AGCGGCAAGT TGCCCCTGTT 
GCTGGCGTTG CCCGGAAAGG CCGTAGAATT TGGGGCTCAC GGATGATTGA TATCCAGACC 
AGGATGGCTG GGCGAGCATT GGAGCTTCTT TATCTGCCAG AGAATAAGCC CTGTTACCTG 
CTGGATATTG GCTGTGGCAC TGGGCTGAGT GGAAGTTATC TGTCAGATGA AGGGCACTAT 
TGGGTGGGCC TGGATATCAG CCCTGCCATG CTGGATGAGG CTGTGGACCG AGAGATAGAG 
GGAGACCTGC TGCTGGGGGA TATGGGCCAG GGCATCCCAT TCAAGCCAGG CACATTTGAT 
GGTTGCATCA GCATTTCTGC TGTGCAGTGG CTCTGTAATG CTAACAAGAA GTCTGAAAAC 
CCTGCCAAGC GCCTGTACTG CTTTTTTGCT TCTCTTTTTT CTGTTCTCGT CCGGGGATCC 
CGAGCTGTCC TGCAGCTGTA CCCTGAGAAC TCAGAGCAGT TGGAGCTGAT CACAACCCAG 
GCCACAAAGG CAGGCTTCTC CGGTGGCATG GTGGTAGACT ACCCTAACAG TGCCAAAGCA 
AAGAAATTCT ACCTCTGCTT GTTTTCTGGG CCTTCGACCT TTATACCAGA GGGGCTGAGT 
GAAAATCAGG ATGAAGTTGA ACCCAGGGAG TCTGTGTTCA CCAATGAGAG GTTCCCATTA 
AGGATGTCGA GGCGGGGAAT GGTGAGGAAG AGTCGGGCAT GGGTGCTGGA GAAGAAGGAG 
CGGCACAGGC GCCAGGGCAG GGAAGTCAGA CCTGACACCC AGTACACCGG CCGCAAGCGC 
AAGCCCCGCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUD23 ( XM_011515778.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514080.1 Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant X3, mRNA.

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CloneID	OHu74728
Clone ID Related Accession (Same CDS sequence)	XM_011515778.1
Accession Version	XM_011515778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable 18S rRNA (guanine-N(7))-methyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACC CCCAGAGCTG TTTTATGACG AGACAGAAGC CCGGAAATAC GTTCGCAAGT 
GAGGGGAGCC TGAATACTGC GGGGCGTCCG GGGGTCGGGA AGCGGCAAGT TGCCCCTGTT 
GCTGGCGTTG CCCGGAAAGG CCGTAGAATT TGGGGCTCAC GGATGATTGA TATCCAGACC 
AGGATGGCTG GGCGAGCATT GGAGCTTCTT TATCTGCCAG AGAATAAGCC CTGTTACCTG 
CTGGATATTG GCTGTGGCAC TGGGCTGAGT GGAAGTTATC TGTCAGATGA AGGGCACTAT 
TGGGTGGGCC TGGATATCAG CCCTGCCATG CTGGATGAGG CTGTGGACCG AGAGATAGAG 
GGAGACCTGC TGCTGGGGGA TATGGGCCAG GGCATCCCAT TCAAGCCAGG CACATTTGAT 
GGTTGCATCA GCATTTCTGC TGTGCAGTGG CTCTGTAATG CTAACAAGAA GTCTGAAAAC 
CCTGCCAAGC GCCTGTACTG CTTTTTTGCT TCTCTTTTTT CTGTTCTCGT CCGGGGATCC 
CGAGCTGTCC TGCAGCTGTA CCCTGAGAAC TCAGAGCAGT TGGAGCTGAT CACAACCCAG 
GCCACAAAGG CAGGCTTCTC CGGTGGCATG GTGGTAGACT ACCCTAACAG TGCCAAAGCA 
AAGAAATTCT ACCTCTGCTT GTTTTCTGGG CCTTCGACCT TTATACCAGA GGTTCCCATT 
AAGGATGTCG AGGCGGGGAA TGGTGAGGAA GAGTCGGGCA TGGGTGCTGG AGAAGAAGGA 
GCGGCACAGG CGCCAGGGCA GGGAAGTCAG ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514080.1
CDS	83..898
Translation	MADPQSCFMTRQKPGNTFASEGSLNTAGRPGVGKRQVAPVAGVARKGRRIWGSRMIDIQTRMAGRALELLYLPENKPCYLLDIGCGTGLSGSYLSDEGHYWVGLDISPAMLDEAVDREIEGDLLLGDMGQGIPFKPGTFDGCISISAVQWLCNANKKSENPAKRLYCFFASLFSVLVRGSRAVLQLYPENSEQLELITTQATKAGFSGGMVVDYPNSAKAKKFYLCLFSGPSTFIPEVPIKDVEAGNGEEESGMGAGEEGAAQAPGQGSQT

Target ORF information:

RefSeq Version	XM_011515778.1
Organism	Homo sapiens(human)
Definition	Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515778.1

ORF Insert Sequence:

ATGGCGGACC CCCAGAGCTG TTTTATGACG AGACAGAAGC CCGGAAATAC GTTCGCAAGT 
GAGGGGAGCC TGAATACTGC GGGGCGTCCG GGGGTCGGGA AGCGGCAAGT TGCCCCTGTT 
GCTGGCGTTG CCCGGAAAGG CCGTAGAATT TGGGGCTCAC GGATGATTGA TATCCAGACC 
AGGATGGCTG GGCGAGCATT GGAGCTTCTT TATCTGCCAG AGAATAAGCC CTGTTACCTG 
CTGGATATTG GCTGTGGCAC TGGGCTGAGT GGAAGTTATC TGTCAGATGA AGGGCACTAT 
TGGGTGGGCC TGGATATCAG CCCTGCCATG CTGGATGAGG CTGTGGACCG AGAGATAGAG 
GGAGACCTGC TGCTGGGGGA TATGGGCCAG GGCATCCCAT TCAAGCCAGG CACATTTGAT 
GGTTGCATCA GCATTTCTGC TGTGCAGTGG CTCTGTAATG CTAACAAGAA GTCTGAAAAC 
CCTGCCAAGC GCCTGTACTG CTTTTTTGCT TCTCTTTTTT CTGTTCTCGT CCGGGGATCC 
CGAGCTGTCC TGCAGCTGTA CCCTGAGAAC TCAGAGCAGT TGGAGCTGAT CACAACCCAG 
GCCACAAAGG CAGGCTTCTC CGGTGGCATG GTGGTAGACT ACCCTAACAG TGCCAAAGCA 
AAGAAATTCT ACCTCTGCTT GTTTTCTGGG CCTTCGACCT TTATACCAGA GGTTCCCATT 
AAGGATGTCG AGGCGGGGAA TGGTGAGGAA GAGTCGGGCA TGGGTGCTGG AGAAGAAGGA 
GCGGCACAGG CGCCAGGGCA GGGAAGTCAG ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUD23 ( XM_011515779.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514081.1 Homo sapiens Williams-Beuren syndrome chromosome region 22 (WBSCR22), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu74729
Clone ID Related Accession (Same CDS sequence)	XM_011515779.1 , XM_011515779.2
Accession Version	XM_011515779.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	probable 18S rRNA (guanine-N(7))-methyltransferase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCC GCGGCCGGCG TCCGGAGCAT GGCGGACCCC CAGAGCTGTT TTATGACGAG 
ACAGAAGCCC GGAAATACGT TCGCAACTCA CGGATGATTG ATATCCAGAC CAGGATGGCT 
GGGCGAGCAT TGGAGCTTCT TTATCTGCCA GAGAATAAGC CCTGTTACCT GCTGGATATT 
GGCTGTGGCA CTGGGCTGAG TGGAAGTTAT CTGTCAGATG AAGGGCACTA TTGGGTGGGC 
CTGGATATCA GCCCTGCCAT GCTGGATGAG GCTGTGGACC GAGAGATAGA GGGAGACCTG 
CTGCTGGGGG ATATGGGCCA GGGCATCCCA TTCAAGCCAG GCACATTTGA TGGTTGCATC 
AGCATTTCTG CTGTGCAGTG GCTCTGTAAT GCTAACAAGA AGTCTGAAAA CCCTGCCAAG 
CGCCTGTACT GCTTTTTTGC TTCTCTTTTT TCTGTTCTCG TCCGGGGATC CCGAGCTGTC 
CTGCAGCTGT ACCCTGAGAA CTCAGAGCAG TTGGAGCTGA TCACAACCCA GGCCACAAAG 
GCAGGCTTCT CCGGTGGCAT GGTGGTAGAC TACCCTAACA GTGCCAAAGC AAAGAAATTC 
TACCTCTGCT TGTTTTCTGG GCCTTCGACC TTTATACCAG AGGTTCCCAT TAAGGATGTC 
GAGGCGGGGA ATGGTGAGGA AGAGTCGGGC ATGGGTGCTG GAGAAGAAGG AGCGGCACAG 
GCGCCAGGGC AGGGAAGTCA GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514081.1
CDS	56..802
Translation	MASRGRRPEHGGPPELFYDETEARKYVRNSRMIDIQTRMAGRALELLYLPENKPCYLLDIGCGTGLSGSYLSDEGHYWVGLDISPAMLDEAVDREIEGDLLLGDMGQGIPFKPGTFDGCISISAVQWLCNANKKSENPAKRLYCFFASLFSVLVRGSRAVLQLYPENSEQLELITTQATKAGFSGGMVVDYPNSAKAKKFYLCLFSGPSTFIPEVPIKDVEAGNGEEESGMGAGEEGAAQAPGQGSQT

Target ORF information:

RefSeq Version	XM_011515779.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Williams-Beuren syndrome chromosome region 22 (WBSCR22), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515779.1

ORF Insert Sequence:

ATGGCGTCCC GCGGCCGGCG TCCGGAGCAT GGCGGACCCC CAGAGCTGTT TTATGACGAG 
ACAGAAGCCC GGAAATACGT TCGCAACTCA CGGATGATTG ATATCCAGAC CAGGATGGCT 
GGGCGAGCAT TGGAGCTTCT TTATCTGCCA GAGAATAAGC CCTGTTACCT GCTGGATATT 
GGCTGTGGCA CTGGGCTGAG TGGAAGTTAT CTGTCAGATG AAGGGCACTA TTGGGTGGGC 
CTGGATATCA GCCCTGCCAT GCTGGATGAG GCTGTGGACC GAGAGATAGA GGGAGACCTG 
CTGCTGGGGG ATATGGGCCA GGGCATCCCA TTCAAGCCAG GCACATTTGA TGGTTGCATC 
AGCATTTCTG CTGTGCAGTG GCTCTGTAAT GCTAACAAGA AGTCTGAAAA CCCTGCCAAG 
CGCCTGTACT GCTTTTTTGC TTCTCTTTTT TCTGTTCTCG TCCGGGGATC CCGAGCTGTC 
CTGCAGCTGT ACCCTGAGAA CTCAGAGCAG TTGGAGCTGA TCACAACCCA GGCCACAAAG 
GCAGGCTTCT CCGGTGGCAT GGTGGTAGAC TACCCTAACA GTGCCAAAGC AAAGAAATTC 
TACCTCTGCT TGTTTTCTGG GCCTTCGACC TTTATACCAG AGGTTCCCAT TAAGGATGTC 
GAGGCGGGGA ATGGTGAGGA AGAGTCGGGC ATGGGTGCTG GAGAAGAAGG AGCGGCACAG 
GCGCCAGGGC AGGGAAGTCA GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUD23 ( XM_011515779.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514081.1 Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu74729
Clone ID Related Accession (Same CDS sequence)	XM_011515779.1 , XM_011515779.2
Accession Version	XM_011515779.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable 18S rRNA (guanine-N(7))-methyltransferase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011515779.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCC GCGGCCGGCG TCCGGAGCAT GGCGGACCCC CAGAGCTGTT TTATGACGAG 
ACAGAAGCCC GGAAATACGT TCGCAACTCA CGGATGATTG ATATCCAGAC CAGGATGGCT 
GGGCGAGCAT TGGAGCTTCT TTATCTGCCA GAGAATAAGC CCTGTTACCT GCTGGATATT 
GGCTGTGGCA CTGGGCTGAG TGGAAGTTAT CTGTCAGATG AAGGGCACTA TTGGGTGGGC 
CTGGATATCA GCCCTGCCAT GCTGGATGAG GCTGTGGACC GAGAGATAGA GGGAGACCTG 
CTGCTGGGGG ATATGGGCCA GGGCATCCCA TTCAAGCCAG GCACATTTGA TGGTTGCATC 
AGCATTTCTG CTGTGCAGTG GCTCTGTAAT GCTAACAAGA AGTCTGAAAA CCCTGCCAAG 
CGCCTGTACT GCTTTTTTGC TTCTCTTTTT TCTGTTCTCG TCCGGGGATC CCGAGCTGTC 
CTGCAGCTGT ACCCTGAGAA CTCAGAGCAG TTGGAGCTGA TCACAACCCA GGCCACAAAG 
GCAGGCTTCT CCGGTGGCAT GGTGGTAGAC TACCCTAACA GTGCCAAAGC AAAGAAATTC 
TACCTCTGCT TGTTTTCTGG GCCTTCGACC TTTATACCAG AGGTTCCCAT TAAGGATGTC 
GAGGCGGGGA ATGGTGAGGA AGAGTCGGGC ATGGGTGCTG GAGAAGAAGG AGCGGCACAG 
GCGCCAGGGC AGGGAAGTCA GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514081.1
CDS	56..802
Translation	MASRGRRPEHGGPPELFYDETEARKYVRNSRMIDIQTRMAGRALELLYLPENKPCYLLDIGCGTGLSGSYLSDEGHYWVGLDISPAMLDEAVDREIEGDLLLGDMGQGIPFKPGTFDGCISISAVQWLCNANKKSENPAKRLYCFFASLFSVLVRGSRAVLQLYPENSEQLELITTQATKAGFSGGMVVDYPNSAKAKKFYLCLFSGPSTFIPEVPIKDVEAGNGEEESGMGAGEEGAAQAPGQGSQT

Target ORF information:

RefSeq Version	XM_011515779.2
Organism	Homo sapiens(human)
Definition	Homo sapiens BUD23 rRNA methyltransferase and ribosome maturation factor (BUD23), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515779.2

ORF Insert Sequence:

ATGGCGTCCC GCGGCCGGCG TCCGGAGCAT GGCGGACCCC CAGAGCTGTT TTATGACGAG 
ACAGAAGCCC GGAAATACGT TCGCAACTCA CGGATGATTG ATATCCAGAC CAGGATGGCT 
GGGCGAGCAT TGGAGCTTCT TTATCTGCCA GAGAATAAGC CCTGTTACCT GCTGGATATT 
GGCTGTGGCA CTGGGCTGAG TGGAAGTTAT CTGTCAGATG AAGGGCACTA TTGGGTGGGC 
CTGGATATCA GCCCTGCCAT GCTGGATGAG GCTGTGGACC GAGAGATAGA GGGAGACCTG 
CTGCTGGGGG ATATGGGCCA GGGCATCCCA TTCAAGCCAG GCACATTTGA TGGTTGCATC 
AGCATTTCTG CTGTGCAGTG GCTCTGTAAT GCTAACAAGA AGTCTGAAAA CCCTGCCAAG 
CGCCTGTACT GCTTTTTTGC TTCTCTTTTT TCTGTTCTCG TCCGGGGATC CCGAGCTGTC 
CTGCAGCTGT ACCCTGAGAA CTCAGAGCAG TTGGAGCTGA TCACAACCCA GGCCACAAAG 
GCAGGCTTCT CCGGTGGCAT GGTGGTAGAC TACCCTAACA GTGCCAAAGC AAAGAAATTC 
TACCTCTGCT TGTTTTCTGG GCCTTCGACC TTTATACCAG AGGTTCCCAT TAAGGATGTC 
GAGGCGGGGA ATGGTGAGGA AGAGTCGGGC ATGGGTGCTG GAGAAGAAGG AGCGGCACAG 
GCGCCAGGGC AGGGAAGTCA GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

WBSCR22 confers oxaliplatin resistance in human colorectal cancer.
Scientific reports7(1)15443(2017 Nov)
Yan D,Tu L,Yuan H,Fang J,Cheng L,Zheng X,Wang X

The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis.
Molecular biology of the cell26(11)2080-95(2015 Jun)
Zorbas C,Nicolas E,Wacheul L,Huvelle E,Heurgu?-Hamard V,Lafontaine DL

WBSCR22/Merm1 is required for late nuclear pre-ribosomal RNA processing and mediates N7-methylation of G1639 in human 18S rRNA.
RNA (New York, N.Y.)21(2)180-7(2015 Feb)
Haag S,Kretschmer J,Bohnsack MT

Knockdown of Merm1/Wbscr22 attenuates sensitivity of H460 non-small cell lung cancer cells to SN-38 and 5-FU without alteration to p53 expression levels.
Molecular medicine reports11(1)295-302(2015 Jan)
Yan D,Zheng X,Tu L,Jia J,Li Q,Cheng L,Wang X

The Stability of Ribosome Biogenesis Factor WBSCR22 Is Regulated by Interaction with TRMT112 via Ubiquitin-Proteasome Pathway.
PloS one10(7)e0133841(2015)
?unap K,Leetsi L,Matsoo M,Kurg R

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オンライン注文

BUD23 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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