POLG2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLG2
Entrez Gene ID	11232
Full Name	DNA polymerase gamma 2, accessory subunit
Synonyms	HP55,MTPOLB,PEOA4,POLB,POLG-BETA,POLGB
General protein information	Preferred Names DNA polymerase gamma 2, accessory subunit Names DNA polymerase subunit gamma-2, mitochondrial DNA polymerase gamma accessory 55 kDa subunit mitochondrial DNA polymerase subunit gamma-2 mitochondrial DNA polymerase, accessory subunit p55 polymerase (DNA directed), gamma 2, accessory subunit polymerase (DNA) gamma 2, accessory subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q23.3
Summary	This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions.[provided by RefSeq, Sep 2009].
Disorder MIM:	604983
Disorder Html:	Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal dominant 4, 610131 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006721651.2	XP_006721714.1	DNA polymerase subunit gamma-2, mitochondrial isoform X2
XM_017024072.1	XP_016879561.1	DNA polymerase subunit gamma-2, mitochondrial isoform X1
NM_007215.3	NP_009146.2	DNA polymerase subunit gamma-2, mitochondrial
NM_007215.4	NP_009146.2	DNA polymerase subunit gamma-2, mitochondrial

Pathways from BioSystems

Homologies

Bos taurus (cattle)	POLG2	NP_001068659.1
Canis lupus familiaris (dog)	POLG2	XP_548032.1
Danio rerio (zebrafish)	polg2	XP_001922359.2
Homo sapiens (human)	POLG2	NP_009146.2
Rattus norvegicus (Norway rat)	Polg2	NP_001100530.1
Gallus gallus (chicken)	POLG2	XP_415670.2
Xenopus tropicalis (tropical clawed frog)	polg2	XP_004916373.1
Pan troglodytes (chimpanzee)	POLG2	XP_001162210.1
Mus musculus (house mouse)	Polg2	NP_056625.2

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Whole exome sequencing identifies a homozygous POLG2 missense variant in an infant with fulminant hepatic failure and mitochondrial DNA depletion.
Varma H, Faust PL, Iglesias AD, Lagana SM, Wou K, Hirano M, DiMauro S, Mansukani MM, Hoff KE, Nagy PL, Copeland WC, Naini AB
European journal of medical genetics59(10)540-5(2016 Oct)

Association of DNA sequence variation in mitochondrial DNA polymerase with mitochondrial DNA synthesis and risk of oral cancer.
Datta S, Ray A, Roy R, Roy B
Gene575(2 Pt 3)650-4(2016 Jan)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This is the first report of a patient with a homozygous mutation in POLG2 and with a clinical presentation of severe hepatic failure and mitochondrial depletion.
Title: Whole exome sequencing identifies a homozygous POLG2 missense variant in an infant with fulminant hepatic failure and mitochondrial DNA depletion.

Results show that polymorphisms at POLG2 and POLRMT increased risk of oral cancer and leukoplakia, respectively, probably modulating synthesis and activity of the enzymes.
Title: Association of DNA sequence variation in mitochondrial DNA polymerase with mitochondrial DNA synthesis and risk of oral cancer.

Mitochondrial DNA (mtDNA) content plays an important role in energy production and sustaining normal physiological function.
Title: Mitochondrial DNA content contributes to healthy aging in Chinese: a study from nonagenarians and centenarians.

The authors describe the molecular characterization of a potential dominant POLG2 mutation (p.R369G) in a patient with autosomal dominant progressive external ophthalmoplegia and multiple mtDNA deletions.
Title: A p.R369G POLG2 mutation associated with adPEO and multiple mtDNA deletions causes decreased affinity between polymerase ? subunits.

Data show that rs17650301 in POLG2 is a good candidate marker for UBC invasiveness in Japanese males.
Title: A polymorphism of the POLG2 gene is genetically associated with the invasiveness of urinary bladder cancer in Japanese males.

The following POLG2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLG2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17869	NM_007215.4 Latest version!	Homo sapiens DNA polymerase gamma 2, accessory subunit (POLG2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu50046	XM_006721651.2 Latest version!	Homo sapiens polymerase (DNA) gamma 2, accessory subunit (POLG2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu86510	XM_017024072.1 Latest version!	Homo sapiens polymerase (DNA) gamma 2, accessory subunit (POLG2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu17869	NM_007215.3	Homo sapiens DNA polymerase gamma 2, accessory subunit (POLG2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLG2 ( NM_007215.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_009146.2 Homo sapiens DNA polymerase gamma 2, accessory subunit (POLG2), mRNA.

Price & Availability↑

CloneID	OHu17869
Clone ID Related Accession (Same CDS sequence)	NM_007215.3 , NM_007215.4
Accession Version	NM_007215.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	DNA polymerase subunit gamma-2, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI826587.1, BC009194.2 and BC000913.2. This sequence is a reference standard in the RefSeqGene project. On Sep 30, 2009 this sequence version replaced NM_007215.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009194.2, SRR1803616.68948.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGCTCTC GTGTAGCCGT CAGGGCCTGC CATAAGGTCT GCAGGTGCCT GTTGTCTGGG 
TTTGGGGGTC GAGTAGATGC GGGGCAGCCG GAGCTGTTGA CGGAAAGGAG TAGCCCCAAA 
GGAGGGCATG TGAAGTCGCA CGCGGAGCTC GAGGGGAACG GCGAGCACCC AGAAGCCCCC 
GGGTCTGGAG AGGGAAGCGA GGCGCTGTTA GAGATCTGTC AGAGAAGGCA TTTCCTAAGT 
GGAAGCAAGC AGCAGCTTAG CCGGGATTCT CTTCTGAGTG GGTGCCACCC CGGCTTCGGA 
CCCTTGGGCG TAGAGTTGCG GAAGAACCTG GCCGCAGAAT GGTGGACCTC GGTGGTGGTG 
TTCAGGGAGC AGGTATTCCC GGTGGACGCC CTCCACCACA AACCAGGCCC TTTGCTACCC 
GGGGACAGTG CCTTCAGGTT AGTTTCTGCA GAAACTCTAC GCGAAATCTT GCAAGACAAA 
GAGCTGAGTA AGGAACAGCT AGTAGCATTT CTTGAGAACG TATTAAAAAC TTCTGGGAAA 
CTACGGGAGA ACCTTCTTCA CGGTGCCTTG GAACACTATG TTAATTGCCT GGATCTGGTA 
AACAAGAGGC TACCTTATGG CCTTGCTCAG ATTGGAGTGT GTTTTCATCC TGTTTTTGAC 
ACTAAGCAGA TACGAAATGG TGTTAAAAGT ATTGGTGAGA AGACTGAAGC TTCGTTAGTA 
TGGTTTACTC CTCCGAGAAC TTCAAACCAG TGGCTTGATT TCTGGTTACG TCATCGACTC 
CAGTGGTGGA GAAAGTTTGC CATGAGTCCA TCTAACTTCA GCAGCAGTGA CTGTCAGGAT 
GAAGAAGGCC GGAAAGGAAA CAAACTTTAC TACAATTTTC CCTGGGGAAA GGAGTTAATA 
GAAACCCTGT GGAACCTAGG AGATCACGAA CTTTTACACA TGTATCCTGG CAATGTGTCT 
AAATTACATG GCCGAGATGG ACGAAAAAAT GTGGTTCCTT GTGTTCTCTC TGTAAATGGG 
GACCTAGACC GAGGCATGCT GGCCTACCTC TATGATTCTT TCCAGCTGAC AGAGAACTCC 
TTTACAAGAA AGAAAAATCT TCATAGAAAG GTACTTAAAC TTCACCCTTG TTTAGCCCCT 
ATTAAGGTTG CTTTGGATGT AGGAAGAGGC CCCACATTGG AACTAAGACA GGTTTGTCAA 
GGGCTATTTA ATGAGTTACT AGAAAATGGG ATTTCTGTGT GGCCTGGTTA TTTGGAAACT 
ATGCAGTCCT CATTGGAACA ACTTTATTCG AAGTATGATG AAATGAGTAT TCTCTTCACA 
GTTTTGGTTA CTGAAACTAC TTTGGAGAAT GGATTAATAC ATCTGAGAAG CAGAGACACC 
ACAATGAAGG AAATGATGCA TATATCCAAA TTAAAAGACT TTTTGATTAA GTATATATCA 
TCAGCTAAGA ATGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009146.2
CDS	99..1556
Translation	MRSRVAVRACHKVCRCLLSGFGGRVDAGQPELLTERSSPKGGHVKSHAELEGNGEHPEAPGSGEGSEALLEICQRRHFLSGSKQQLSRDSLLSGCHPGFGPLGVELRKNLAAEWWTSVVVFREQVFPVDALHHKPGPLLPGDSAFRLVSAETLREILQDKELSKEQLVAFLENVLKTSGKLRENLLHGALEHYVNCLDLVNKRLPYGLAQIGVCFHPVFDTKQIRNGVKSIGEKTEASLVWFTPPRTSNQWLDFWLRHRLQWWRKFAMSPSNFSSSDCQDEEGRKGNKLYYNFPWGKELIETLWNLGDHELLHMYPGNVSKLHGRDGRKNVVPCVLSVNGDLDRGMLAYLYDSFQLTENSFTRKKNLHRKVLKLHPCLAPIKVALDVGRGPTLELRQVCQGLFNELLENGISVWPGYLETMQSSLEQLYSKYDEMSILFTVLVTETTLENGLIHLRSRDTTMKEMMHISKLKDFLIKYISSAKNV

Target ORF information:

RefSeq Version	NM_007215.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase gamma 2, accessory subunit (POLG2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007215.3

ORF Insert Sequence:

ATGCGCTCTC GTGTAGCCGT CAGGGCCTGC CATAAGGTCT GCAGGTGCCT GTTGTCTGGG 
TTTGGGGGTC GAGTAGATGC GGGGCAGCCG GAGCTGTTGA CGGAAAGGAG TAGCCCCAAA 
GGAGGGCATG TGAAGTCGCA CGCGGAGCTC GAGGGGAACG GCGAGCACCC AGAAGCCCCC 
GGGTCTGGAG AGGGAAGCGA GGCGCTGTTA GAGATCTGTC AGAGAAGGCA TTTCCTAAGT 
GGAAGCAAGC AGCAGCTTAG CCGGGATTCT CTTCTGAGTG GGTGCCACCC CGGCTTCGGA 
CCCTTGGGCG TAGAGTTGCG GAAGAACCTG GCCGCAGAAT GGTGGACCTC GGTGGTGGTG 
TTCAGGGAGC AGGTATTCCC GGTGGACGCC CTCCACCACA AACCAGGCCC TTTGCTACCC 
GGGGACAGTG CCTTCAGGTT AGTTTCTGCA GAAACTCTAC GCGAAATCTT GCAAGACAAA 
GAGCTGAGTA AGGAACAGCT AGTAGCATTT CTTGAGAACG TATTAAAAAC TTCTGGGAAA 
CTACGGGAGA ACCTTCTTCA CGGTGCCTTG GAACACTATG TTAATTGCCT GGATCTGGTA 
AACAAGAGGC TACCTTATGG CCTTGCTCAG ATTGGAGTGT GTTTTCATCC TGTTTTTGAC 
ACTAAGCAGA TACGAAATGG TGTTAAAAGT ATTGGTGAGA AGACTGAAGC TTCGTTAGTA 
TGGTTTACTC CTCCGAGAAC TTCAAACCAG TGGCTTGATT TCTGGTTACG TCATCGACTC 
CAGTGGTGGA GAAAGTTTGC CATGAGTCCA TCTAACTTCA GCAGCAGTGA CTGTCAGGAT 
GAAGAAGGCC GGAAAGGAAA CAAACTTTAC TACAATTTTC CCTGGGGAAA GGAGTTAATA 
GAAACCCTGT GGAACCTAGG AGATCACGAA CTTTTACACA TGTATCCTGG CAATGTGTCT 
AAATTACATG GCCGAGATGG ACGAAAAAAT GTGGTTCCTT GTGTTCTCTC TGTAAATGGG 
GACCTAGACC GAGGCATGCT GGCCTACCTC TATGATTCTT TCCAGCTGAC AGAGAACTCC 
TTTACAAGAA AGAAAAATCT TCATAGAAAG GTACTTAAAC TTCACCCTTG TTTAGCCCCT 
ATTAAGGTTG CTTTGGATGT AGGAAGAGGC CCCACATTGG AACTAAGACA GGTTTGTCAA 
GGGCTATTTA ATGAGTTACT AGAAAATGGG ATTTCTGTGT GGCCTGGTTA TTTGGAAACT 
ATGCAGTCCT CATTGGAACA ACTTTATTCG AAGTATGATG AAATGAGTAT TCTCTTCACA 
GTTTTGGTTA CTGAAACTAC TTTGGAGAAT GGATTAATAC ATCTGAGAAG CAGAGACACC 
ACAATGAAGG AAATGATGCA TATATCCAAA TTAAAAGACT TTTTGATTAA GTATATATCA 
TCAGCTAAGA ATGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLG2 ( NM_007215.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_009146.2 Homo sapiens DNA polymerase gamma 2, accessory subunit (POLG2), mRNA.

Price & Availability↑

CloneID	OHu17869
Clone ID Related Accession (Same CDS sequence)	NM_007215.3 , NM_007215.4
Accession Version	NM_007215.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	DNA polymerase subunit gamma-2, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI826587.1, BC009194.2 and BC000913.2. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_007215.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.643997.1, SRR1803616.68948.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000539111.7/ ENSP00000442563.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGCTCTC GTGTAGCCGT CAGGGCCTGC CATAAGGTCT GCAGGTGCCT GTTGTCTGGG 
TTTGGGGGTC GAGTAGATGC GGGGCAGCCG GAGCTGTTGA CGGAAAGGAG TAGCCCCAAA 
GGAGGGCATG TGAAGTCGCA CGCGGAGCTC GAGGGGAACG GCGAGCACCC AGAAGCCCCC 
GGGTCTGGAG AGGGAAGCGA GGCGCTGTTA GAGATCTGTC AGAGAAGGCA TTTCCTAAGT 
GGAAGCAAGC AGCAGCTTAG CCGGGATTCT CTTCTGAGTG GGTGCCACCC CGGCTTCGGA 
CCCTTGGGCG TAGAGTTGCG GAAGAACCTG GCCGCAGAAT GGTGGACCTC GGTGGTGGTG 
TTCAGGGAGC AGGTATTCCC GGTGGACGCC CTCCACCACA AACCAGGCCC TTTGCTACCC 
GGGGACAGTG CCTTCAGGTT AGTTTCTGCA GAAACTCTAC GCGAAATCTT GCAAGACAAA 
GAGCTGAGTA AGGAACAGCT AGTAGCATTT CTTGAGAACG TATTAAAAAC TTCTGGGAAA 
CTACGGGAGA ACCTTCTTCA CGGTGCCTTG GAACACTATG TTAATTGCCT GGATCTGGTA 
AACAAGAGGC TACCTTATGG CCTTGCTCAG ATTGGAGTGT GTTTTCATCC TGTTTTTGAC 
ACTAAGCAGA TACGAAATGG TGTTAAAAGT ATTGGTGAGA AGACTGAAGC TTCGTTAGTA 
TGGTTTACTC CTCCGAGAAC TTCAAACCAG TGGCTTGATT TCTGGTTACG TCATCGACTC 
CAGTGGTGGA GAAAGTTTGC CATGAGTCCA TCTAACTTCA GCAGCAGTGA CTGTCAGGAT 
GAAGAAGGCC GGAAAGGAAA CAAACTTTAC TACAATTTTC CCTGGGGAAA GGAGTTAATA 
GAAACCCTGT GGAACCTAGG AGATCACGAA CTTTTACACA TGTATCCTGG CAATGTGTCT 
AAATTACATG GCCGAGATGG ACGAAAAAAT GTGGTTCCTT GTGTTCTCTC TGTAAATGGG 
GACCTAGACC GAGGCATGCT GGCCTACCTC TATGATTCTT TCCAGCTGAC AGAGAACTCC 
TTTACAAGAA AGAAAAATCT TCATAGAAAG GTACTTAAAC TTCACCCTTG TTTAGCCCCT 
ATTAAGGTTG CTTTGGATGT AGGAAGAGGC CCCACATTGG AACTAAGACA GGTTTGTCAA 
GGGCTATTTA ATGAGTTACT AGAAAATGGG ATTTCTGTGT GGCCTGGTTA TTTGGAAACT 
ATGCAGTCCT CATTGGAACA ACTTTATTCG AAGTATGATG AAATGAGTAT TCTCTTCACA 
GTTTTGGTTA CTGAAACTAC TTTGGAGAAT GGATTAATAC ATCTGAGAAG CAGAGACACC 
ACAATGAAGG AAATGATGCA TATATCCAAA TTAAAAGACT TTTTGATTAA GTATATATCA 
TCAGCTAAGA ATGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009146.2
CDS	87..1544
Translation	MRSRVAVRACHKVCRCLLSGFGGRVDAGQPELLTERSSPKGGHVKSHAELEGNGEHPEAPGSGEGSEALLEICQRRHFLSGSKQQLSRDSLLSGCHPGFGPLGVELRKNLAAEWWTSVVVFREQVFPVDALHHKPGPLLPGDSAFRLVSAETLREILQDKELSKEQLVAFLENVLKTSGKLRENLLHGALEHYVNCLDLVNKRLPYGLAQIGVCFHPVFDTKQIRNGVKSIGEKTEASLVWFTPPRTSNQWLDFWLRHRLQWWRKFAMSPSNFSSSDCQDEEGRKGNKLYYNFPWGKELIETLWNLGDHELLHMYPGNVSKLHGRDGRKNVVPCVLSVNGDLDRGMLAYLYDSFQLTENSFTRKKNLHRKVLKLHPCLAPIKVALDVGRGPTLELRQVCQGLFNELLENGISVWPGYLETMQSSLEQLYSKYDEMSILFTVLVTETTLENGLIHLRSRDTTMKEMMHISKLKDFLIKYISSAKNV

Target ORF information:

RefSeq Version	NM_007215.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase gamma 2, accessory subunit (POLG2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007215.4

ORF Insert Sequence:

ATGCGCTCTC GTGTAGCCGT CAGGGCCTGC CATAAGGTCT GCAGGTGCCT GTTGTCTGGG 
TTTGGGGGTC GAGTAGATGC GGGGCAGCCG GAGCTGTTGA CGGAAAGGAG TAGCCCCAAA 
GGAGGGCATG TGAAGTCGCA CGCGGAGCTC GAGGGGAACG GCGAGCACCC AGAAGCCCCC 
GGGTCTGGAG AGGGAAGCGA GGCGCTGTTA GAGATCTGTC AGAGAAGGCA TTTCCTAAGT 
GGAAGCAAGC AGCAGCTTAG CCGGGATTCT CTTCTGAGTG GGTGCCACCC CGGCTTCGGA 
CCCTTGGGCG TAGAGTTGCG GAAGAACCTG GCCGCAGAAT GGTGGACCTC GGTGGTGGTG 
TTCAGGGAGC AGGTATTCCC GGTGGACGCC CTCCACCACA AACCAGGCCC TTTGCTACCC 
GGGGACAGTG CCTTCAGGTT AGTTTCTGCA GAAACTCTAC GCGAAATCTT GCAAGACAAA 
GAGCTGAGTA AGGAACAGCT AGTAGCATTT CTTGAGAACG TATTAAAAAC TTCTGGGAAA 
CTACGGGAGA ACCTTCTTCA CGGTGCCTTG GAACACTATG TTAATTGCCT GGATCTGGTA 
AACAAGAGGC TACCTTATGG CCTTGCTCAG ATTGGAGTGT GTTTTCATCC TGTTTTTGAC 
ACTAAGCAGA TACGAAATGG TGTTAAAAGT ATTGGTGAGA AGACTGAAGC TTCGTTAGTA 
TGGTTTACTC CTCCGAGAAC TTCAAACCAG TGGCTTGATT TCTGGTTACG TCATCGACTC 
CAGTGGTGGA GAAAGTTTGC CATGAGTCCA TCTAACTTCA GCAGCAGTGA CTGTCAGGAT 
GAAGAAGGCC GGAAAGGAAA CAAACTTTAC TACAATTTTC CCTGGGGAAA GGAGTTAATA 
GAAACCCTGT GGAACCTAGG AGATCACGAA CTTTTACACA TGTATCCTGG CAATGTGTCT 
AAATTACATG GCCGAGATGG ACGAAAAAAT GTGGTTCCTT GTGTTCTCTC TGTAAATGGG 
GACCTAGACC GAGGCATGCT GGCCTACCTC TATGATTCTT TCCAGCTGAC AGAGAACTCC 
TTTACAAGAA AGAAAAATCT TCATAGAAAG GTACTTAAAC TTCACCCTTG TTTAGCCCCT 
ATTAAGGTTG CTTTGGATGT AGGAAGAGGC CCCACATTGG AACTAAGACA GGTTTGTCAA 
GGGCTATTTA ATGAGTTACT AGAAAATGGG ATTTCTGTGT GGCCTGGTTA TTTGGAAACT 
ATGCAGTCCT CATTGGAACA ACTTTATTCG AAGTATGATG AAATGAGTAT TCTCTTCACA 
GTTTTGGTTA CTGAAACTAC TTTGGAGAAT GGATTAATAC ATCTGAGAAG CAGAGACACC 
ACAATGAAGG AAATGATGCA TATATCCAAA TTAAAAGACT TTTTGATTAA GTATATATCA 
TCAGCTAAGA ATGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLG2 ( XM_006721651.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006721714.1 Homo sapiens polymerase (DNA) gamma 2, accessory subunit (POLG2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu50046
Clone ID Related Accession (Same CDS sequence)	XM_006721651.2
Accession Version	XM_006721651.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DNA polymerase subunit gamma-2, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006721651.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTCTC GTGTAGCCGT CAGGGCCTGC CATAAGGTCT GCAGGTGCCT GTTGTCTGGG 
TTTGGGGGTC GAGTAGATGC GGGGCAGCCG GAGCTGTTGA CGGAAAGGAG TAGCCCCAAA 
GGAGGGCATG TGAAGTCGCA CGCGGAGCTC GAGGGGAACG GCGAGCACCC AGAAGCCCCC 
GGGTCTGGAG AGGGAAGCGA GGCGCTGTTA GAGATCTGTC AGAGAAGGCA TTTCCTAAGT 
GGAAGCAAGC AGCAGCTTAG CCGGGATTCT CTTCTGAGTG GGTGCCACCC CGGCTTCGGA 
CCCTTGGGCG TAGAGTTGCG GAAGAACCTG GCCGCAGAAT GGTGGACCTC GGTGGTGGTG 
TTCAGGGAGC AGGTATTCCC GGTGGACGCC CTCCACCACA AACCAGGCCC TTTGCTACCC 
GGGGACAGTG CCTTCAGGTT AGTTTCTGCA GAAACTCTAC GCGAAATCTT GCAAGACAAA 
GAGCTGAGTA AGGAACAGCT AGTAGCATTT CTTGAGAACG TATTAAAAAC TTCTGGGAAA 
CTACGGGAGA ACCTTCTTCA CGGTGCCTTG GAACACTATG TTAATTGCCT GGATCTGGTA 
AACAAGAGGC TACCTTATGG CCTTGCTCAG ATTGGAGTGT GTTTTCATCC TGTTTTTGAC 
ACTAAGCAGA TACGAAATGG TGTTAAAAGT ATTGGTGAGA AGACTGAAGC TTCGTTAGTA 
TGGTTTACTC CTCCGAGAAC TTCAAACCAG TGGCTTGATT TCTGGTTACG TCATCGACTC 
CAGTGGTGGA GAAAGTTTGC CATGAGTCCA TCTAACTTCA GCAGCAGTGA CTGTCAGGAT 
GAAGAAGGCC GGAAAGGAAA CAAACTTTAC TACAATTTTC CCTGGGGAAA GGAGTTAATA 
GAAACCCTGT GGAACCTAGG AGATCACGAA CTTTTACACA TGTATCCTGG CAATGTGTCT 
AAATTACATA TGCAGAGACA CTCACCTACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006721714.1
CDS	52..1044
Translation	MRSRVAVRACHKVCRCLLSGFGGRVDAGQPELLTERSSPKGGHVKSHAELEGNGEHPEAPGSGEGSEALLEICQRRHFLSGSKQQLSRDSLLSGCHPGFGPLGVELRKNLAAEWWTSVVVFREQVFPVDALHHKPGPLLPGDSAFRLVSAETLREILQDKELSKEQLVAFLENVLKTSGKLRENLLHGALEHYVNCLDLVNKRLPYGLAQIGVCFHPVFDTKQIRNGVKSIGEKTEASLVWFTPPRTSNQWLDFWLRHRLQWWRKFAMSPSNFSSSDCQDEEGRKGNKLYYNFPWGKELIETLWNLGDHELLHMYPGNVSKLHMQRHSPT

Target ORF information:

RefSeq Version	XM_006721651.2
Organism	Homo sapiens(human)
Definition	Homo sapiens polymerase (DNA) gamma 2, accessory subunit (POLG2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006721651.2

ORF Insert Sequence:

ATGCGCTCTC GTGTAGCCGT CAGGGCCTGC CATAAGGTCT GCAGGTGCCT GTTGTCTGGG 
TTTGGGGGTC GAGTAGATGC GGGGCAGCCG GAGCTGTTGA CGGAAAGGAG TAGCCCCAAA 
GGAGGGCATG TGAAGTCGCA CGCGGAGCTC GAGGGGAACG GCGAGCACCC AGAAGCCCCC 
GGGTCTGGAG AGGGAAGCGA GGCGCTGTTA GAGATCTGTC AGAGAAGGCA TTTCCTAAGT 
GGAAGCAAGC AGCAGCTTAG CCGGGATTCT CTTCTGAGTG GGTGCCACCC CGGCTTCGGA 
CCCTTGGGCG TAGAGTTGCG GAAGAACCTG GCCGCAGAAT GGTGGACCTC GGTGGTGGTG 
TTCAGGGAGC AGGTATTCCC GGTGGACGCC CTCCACCACA AACCAGGCCC TTTGCTACCC 
GGGGACAGTG CCTTCAGGTT AGTTTCTGCA GAAACTCTAC GCGAAATCTT GCAAGACAAA 
GAGCTGAGTA AGGAACAGCT AGTAGCATTT CTTGAGAACG TATTAAAAAC TTCTGGGAAA 
CTACGGGAGA ACCTTCTTCA CGGTGCCTTG GAACACTATG TTAATTGCCT GGATCTGGTA 
AACAAGAGGC TACCTTATGG CCTTGCTCAG ATTGGAGTGT GTTTTCATCC TGTTTTTGAC 
ACTAAGCAGA TACGAAATGG TGTTAAAAGT ATTGGTGAGA AGACTGAAGC TTCGTTAGTA 
TGGTTTACTC CTCCGAGAAC TTCAAACCAG TGGCTTGATT TCTGGTTACG TCATCGACTC 
CAGTGGTGGA GAAAGTTTGC CATGAGTCCA TCTAACTTCA GCAGCAGTGA CTGTCAGGAT 
GAAGAAGGCC GGAAAGGAAA CAAACTTTAC TACAATTTTC CCTGGGGAAA GGAGTTAATA 
GAAACCCTGT GGAACCTAGG AGATCACGAA CTTTTACACA TGTATCCTGG CAATGTGTCT 
AAATTACATA TGCAGAGACA CTCACCTACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLG2 ( XM_017024072.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879561.1 Homo sapiens polymerase (DNA) gamma 2, accessory subunit (POLG2), transcript variant X3, mRNA.

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CloneID	OHu86510
Clone ID Related Accession (Same CDS sequence)	XM_017024072.1
Accession Version	XM_017024072.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DNA polymerase subunit gamma-2, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTCTC GTGTAGCCGT CAGGGCCTGC CATAAGGTCT GCAGGTGCCT GTTGTCTGGG 
TTTGGGGGTC GAGTAGATGC GGGGCAGCCG GAGCTGTTGA CGGAAAGGAG TAGCCCCAAA 
GGAGGGCATG TGAAGTCGCA CGCGGAGCTC GAGGGGAACG GCGAGCACCC AGAAGCCCCC 
GGGTCTGGAG AGGGAAGCGA GGCGCTGTTA GAGATCTGTC AGAGAAGGCA TTTCCTAAGT 
GGAAGCAAGC AGCAGCTTAG CCGGGATTCT CTTCTGAGTG GGTGCCACCC CGGCTTCGGA 
CCCTTGGGCG TAGAGTTGCG GAAGAACCTG GCCGCAGAAT GGTGGACCTC GGTGGTGGTG 
TTCAGGGAGC AGGTATTCCC GGTGGACGCC CTCCACCACA AACCAGGCCC TTTGCTACCC 
GGGGACAGTG CCTTCAGGTT AGTTTCTGCA GAAACTCTAC GCGAAATCTT GCAAGACAAA 
GAGCTGAGTA AGGAACAGCT AGTAGCATTT CTTGAGAACG TATTAAAAAC TTCTGGGAAA 
CTACGGGAGA ACCTTCTTCA CGGTGCCTTG GAACACTATG TTAATTGCCT GGATCTGGTA 
AACAAGAGGC TACCTTATGG CCTTGCTCAG ATTGGAGTGT GTTTTCATCC TGTTTTTGAC 
ACTAAGCAGA TACGAAATGG TGTTAAAAGT ATTGGTGAGA AGACTGAAGC TTCGTTAGTA 
TGGTTTACTC CTCCGAGAAC TTCAAACCAG TGGCTTGATT TCTGGTTACG TCATCGACTC 
CAGTGGTGGA GAAAGTTTGC CATGAGTCCA TCTAACTTCA GCAGCAGTGA CTGTCAGGAT 
GAAGAAGGCC GGAAAGGAAA CAAACTTTAC TACAATTTTC CCTGGGGAAA GGAGTTAATA 
GAAACCCTGT GGAACCTAGG AGATCACGAA CTTTTACACA TGTATCCTGG CAATGTGTCT 
AAATTACATG GCCGAGATGG ACGAAAAAAT GTGGTTCCTT GTGTTCTCTC TGTAAATGGG 
GACCTAGACC GAGGCATGCT GGCCTACCTC TATGATTCTT TCCAGCTGAC AGAGAACTCC 
TTTACAAGAA AGAAAAATCT TCATAGAAAG GTACTTAAAC TTCACCCTTG TTTAGCCCCT 
ATTAAGGTTG CTTTGGATGT AGGAAGAGGC CCCACATTGG AACTAAGACA GGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879561.1
CDS	52..1248
Translation	MRSRVAVRACHKVCRCLLSGFGGRVDAGQPELLTERSSPKGGHVKSHAELEGNGEHPEAPGSGEGSEALLEICQRRHFLSGSKQQLSRDSLLSGCHPGFGPLGVELRKNLAAEWWTSVVVFREQVFPVDALHHKPGPLLPGDSAFRLVSAETLREILQDKELSKEQLVAFLENVLKTSGKLRENLLHGALEHYVNCLDLVNKRLPYGLAQIGVCFHPVFDTKQIRNGVKSIGEKTEASLVWFTPPRTSNQWLDFWLRHRLQWWRKFAMSPSNFSSSDCQDEEGRKGNKLYYNFPWGKELIETLWNLGDHELLHMYPGNVSKLHGRDGRKNVVPCVLSVNGDLDRGMLAYLYDSFQLTENSFTRKKNLHRKVLKLHPCLAPIKVALDVGRGPTLELRQV

Target ORF information:

RefSeq Version	XM_017024072.1
Organism	Homo sapiens(human)
Definition	Homo sapiens polymerase (DNA) gamma 2, accessory subunit (POLG2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024072.1

ORF Insert Sequence:

ATGCGCTCTC GTGTAGCCGT CAGGGCCTGC CATAAGGTCT GCAGGTGCCT GTTGTCTGGG 
TTTGGGGGTC GAGTAGATGC GGGGCAGCCG GAGCTGTTGA CGGAAAGGAG TAGCCCCAAA 
GGAGGGCATG TGAAGTCGCA CGCGGAGCTC GAGGGGAACG GCGAGCACCC AGAAGCCCCC 
GGGTCTGGAG AGGGAAGCGA GGCGCTGTTA GAGATCTGTC AGAGAAGGCA TTTCCTAAGT 
GGAAGCAAGC AGCAGCTTAG CCGGGATTCT CTTCTGAGTG GGTGCCACCC CGGCTTCGGA 
CCCTTGGGCG TAGAGTTGCG GAAGAACCTG GCCGCAGAAT GGTGGACCTC GGTGGTGGTG 
TTCAGGGAGC AGGTATTCCC GGTGGACGCC CTCCACCACA AACCAGGCCC TTTGCTACCC 
GGGGACAGTG CCTTCAGGTT AGTTTCTGCA GAAACTCTAC GCGAAATCTT GCAAGACAAA 
GAGCTGAGTA AGGAACAGCT AGTAGCATTT CTTGAGAACG TATTAAAAAC TTCTGGGAAA 
CTACGGGAGA ACCTTCTTCA CGGTGCCTTG GAACACTATG TTAATTGCCT GGATCTGGTA 
AACAAGAGGC TACCTTATGG CCTTGCTCAG ATTGGAGTGT GTTTTCATCC TGTTTTTGAC 
ACTAAGCAGA TACGAAATGG TGTTAAAAGT ATTGGTGAGA AGACTGAAGC TTCGTTAGTA 
TGGTTTACTC CTCCGAGAAC TTCAAACCAG TGGCTTGATT TCTGGTTACG TCATCGACTC 
CAGTGGTGGA GAAAGTTTGC CATGAGTCCA TCTAACTTCA GCAGCAGTGA CTGTCAGGAT 
GAAGAAGGCC GGAAAGGAAA CAAACTTTAC TACAATTTTC CCTGGGGAAA GGAGTTAATA 
GAAACCCTGT GGAACCTAGG AGATCACGAA CTTTTACACA TGTATCCTGG CAATGTGTCT 
AAATTACATG GCCGAGATGG ACGAAAAAAT GTGGTTCCTT GTGTTCTCTC TGTAAATGGG 
GACCTAGACC GAGGCATGCT GGCCTACCTC TATGATTCTT TCCAGCTGAC AGAGAACTCC 
TTTACAAGAA AGAAAAATCT TCATAGAAAG GTACTTAAAC TTCACCCTTG TTTAGCCCCT 
ATTAAGGTTG CTTTGGATGT AGGAAGAGGC CCCACATTGG AACTAAGACA GGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Whole exome sequencing identifies a homozygous POLG2 missense variant in an infant with fulminant hepatic failure and mitochondrial DNA depletion.
European journal of medical genetics59(10)540-5(2016 Oct)
Varma H,Faust PL,Iglesias AD,Lagana SM,Wou K,Hirano M,DiMauro S,Mansukani MM,Hoff KE,Nagy PL,Copeland WC,Naini AB

Association of DNA sequence variation in mitochondrial DNA polymerase with mitochondrial DNA synthesis and risk of oral cancer.
Gene575(2 Pt 3)650-4(2016 Jan)
Datta S,Ray A,Roy R,Roy B

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

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POLG2 cDNA ORF clone, Homo sapiens(human)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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