gRNAs
The following MMP24-AS1-EDEM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MMP24-AS1-EDEM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu103424 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001355008.1 , NM_001355008.2 | |
| Accession Version | NM_001355008.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-12-21 | |
| Organism | Homo sapiens(human) | |
| Product | ER degradation-enhancing alpha-mannosidase-like protein 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL121753.32, AL356652.19 and AL135844.9. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.22626.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## readthrough transcript :: includes exons from GeneID 55741, 101410538 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTTCTACC ACGCCTACGA CAGCTACCTG GAGAATGCCT TTCCCTTCGA TGAGCTGCGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001341937.1 |
| CDS | 737..2350 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001355008.1 |
1 | ATGTTCTACC ACGCCTACGA CAGCTACCTG GAGAATGCCT TTCCCTTCGA TGAGCTGCGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu103424 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001355008.1 , NM_001355008.2 | |
| Accession Version | NM_001355008.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-06-01 | |
| Organism | Homo sapiens(human) | |
| Product | ER degradation-enhancing alpha-mannosidase-like protein 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL121753.32, AL356652.19 and AL135844.9. On Jun 2, 2019 this sequence version replaced NM_001355008.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.22626.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## readthrough transcript :: includes exons from GeneID 55741, 101410538 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTTCTACC ACGCCTACGA CAGCTACCTG GAGAATGCCT TTCCCTTCGA TGAGCTGCGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001341937.1 |
| CDS | 681..2294 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001355008.2 |
1 | ATGTTCTACC ACGCCTACGA CAGCTACCTG GAGAATGCCT TTCCCTTCGA TGAGCTGCGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step. |
The role of EDEM2 compared with EDEM1?in ricin transport from the endoplasmic reticulum to the cytosol. |
Human EDEM2, a novel homolog of family 47 glycosidases, is involved in ER-associated degradation of glycoproteins. |
A novel stress-induced EDEM variant regulating endoplasmic reticulum-associated glycoprotein degradation. |
The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. |
