CETN3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CETN3
Entrez Gene ID	1070
Full Name	centrin 3
Synonyms	CDC31,CEN3
General protein information	Preferred Names centrin 3 Names centrin-3 CDC31 yeast homolog EF-hand superfamily member centrin, EF-hand protein, 3 (CDC31 homolog, yeast)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q14.3
Summary	The protein encoded by this gene contains four EF-hand calcium binding domains, and is a member of the centrin protein family. Centrins are evolutionarily conserved proteins similar to the CDC31 protein of S. cerevisiae. Yeast CDC31 is located at the centrosome of interphase and mitotic cells, where it plays a fundamental role in centrosome duplication and separation. Multiple forms of the proteins similar to the yeast centrin have been identified in human and other mammalian cells, some of which have been shown to be associated with centrosome fractions. This protein appears to be one of the most abundant centrins associated with centrosome, which suggests a similar function to its yeast counterpart. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014].
Disorder MIM:	602907

mRNA and Protein(s)

mRNA	Protein	Name
NM_001297768.1	NP_001284697.1	centrin-3 isoform 3
NM_004365.3	NP_004356.2	centrin-3 isoform 2
NM_001297765.1	NP_001284694.1	centrin-3 isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CETN3	XP_003950560.1
Canis lupus familiaris (dog)	CETN3	XP_546032.2
Kluyveromyces lactis	KLLA0C09669g	XP_452629.1
Schizosaccharomyces pombe (fission yeast)	cdc31	NP_587797.1
Homo sapiens (human)	CETN3	NP_004356.2
Bos taurus (cattle)	CETN3	NP_001069444.1
Eremothecium gossypii	AGOS_AAL110C	NP_982432.1
Gallus gallus (chicken)	CETN3	XP_424696.2
Saccharomyces cerevisiae (baker's yeast)	CDC31	NP_014900.3
Xenopus tropicalis (tropical clawed frog)	cetn3	NP_001016387.1
	CETN3	XP_001088516.1
Mus musculus (house mouse)	Cetn3	NP_031710.1
Danio rerio (zebrafish)	cetn3	NP_001018335.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface.
Gupta GD, Coyaud ?, Gon?alves J, Mojarad BA, Liu Y, Wu Q, Gheiratmand L, Comartin D, Tkach JM, Cheung SW, Bashkurov M, Hasegan M, Knight JD, Lin ZY, Schueler M, Hildebrandt F, Moffat J, Gingras AC, Raught B, Pelletier L
Cell163(6)1484-99(2015 Dec)

MDM1 is a microtubule-binding protein that negatively regulates centriole duplication.
Van de Mark D, Kong D, Loncarek J, Stearns T
Molecular biology of the cell26(21)3788-802(2015 Nov)

Centrin 3 is an inhibitor of centrosomal Mps1 and antagonizes centrin 2 function.
Sawant DB, Majumder S, Perkins JL, Yang CH, Eyers PA, Fisk HA
Molecular biology of the cell26(21)3741-53(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cetn3 inhibits Mps1 autophosphorylation at Thr-676, a known site of T-loop autoactivation, and interferes with Mps1-dependent phosphorylation of Cetn2. The cellular overexpression of Cetn3 attenuates the incorporation of Cetn2 into centrioles and centrosome reduplication, whereas depletion of Cetn3 generates extra centrioles.
Title: Centrin 3 is an inhibitor of centrosomal Mps1 and antagonizes centrin 2 function.

Observational study of gene-disease association. (HuGE Navigator)
Title: Centrosome-related genes, genetic variation, and risk of breast cancer.

could be classified as a Ca(2+) sensor protein
Title: Calcium and magnesium binding to human centrin 3 and interaction with target peptides.

The following CETN3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CETN3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04264	NM_004365.3 Latest version!	Homo sapiens centrin 3 (CETN3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu39408	NM_001297765.1 Latest version!	Homo sapiens centrin 3 (CETN3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu56825	NM_001297768.1 Latest version!	Homo sapiens centrin 3 (CETN3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CETN3 ( NM_004365.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004356.2 Homo sapiens centrin 3 (CETN3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04264
Clone ID Related Accession (Same CDS sequence)	NM_004365.3
Accession Version	NM_004365.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	centrin-3 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB998764.1, BC005383.1 and BQ000279.1. On Jul 10, 2014 this sequence version replaced NM_004365.2. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.234342.1, SRR1660805.250364.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMN03267752 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGTTTAG CTCTGAGAAG TGAGCTTGTA GTGGACAAAA CAAAGAGGAA AAAAAGAAGA 
GAACTGTCTG AGGAACAGAA ACAAGAAATT AAAGATGCTT TTGAACTATT TGATACAGAC 
AAAGATGAAG CAATAGATTA TCATGAATTA AAGGTGGCAA TGAGAGCCTT GGGGTTTGAT 
GTAAAAAAAG CTGATGTACT GAAGATTCTT AAAGATTATG ACAGAGAAGC CACAGGGAAA 
ATCACCTTTG AAGATTTTAA TGAAGTTGTG ACAGACTGGA TATTGGAAAG AGATCCCCAT 
GAAGAAATAC TCAAGGCATT TAAACTATTT GATGATGATG ATTCAGGTAA AATAAGCTTG 
AGGAATTTGC GACGTGTTGC TAGAGAATTG GGTGAAAACA TGAGTGATGA AGAACTTCGA 
GCTATGATAG AAGAATTTGA CAAAGATGGT GATGGAGAAA TAAACCAAGA GGAGTTCATT 
GCTATTATGA CTGGTGACAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004356.2
CDS	126..629
Translation	MSLALRSELVVDKTKRKKRRELSEEQKQEIKDAFELFDTDKDEAIDYHELKVAMRALGFDVKKADVLKILKDYDREATGKITFEDFNEVVTDWILERDPHEEILKAFKLFDDDDSGKISLRNLRRVARELGENMSDEELRAMIEEFDKDGDGEINQEEFIAIMTGDI

Target ORF information:

RefSeq Version	NM_004365.3
Organism	Homo sapiens(human)
Definition	Homo sapiens centrin 3 (CETN3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004365.3

ORF Insert Sequence:

ATGAGTTTAG CTCTGAGAAG TGAGCTTGTA GTGGACAAAA CAAAGAGGAA AAAAAGAAGA 
GAACTGTCTG AGGAACAGAA ACAAGAAATT AAAGATGCTT TTGAACTATT TGATACAGAC 
AAAGATGAAG CAATAGATTA TCATGAATTA AAGGTGGCAA TGAGAGCCTT GGGGTTTGAT 
GTAAAAAAAG CTGATGTACT GAAGATTCTT AAAGATTATG ACAGAGAAGC CACAGGGAAA 
ATCACCTTTG AAGATTTTAA TGAAGTTGTG ACAGACTGGA TATTGGAAAG AGATCCCCAT 
GAAGAAATAC TCAAGGCATT TAAACTATTT GATGATGATG ATTCAGGTAA AATAAGCTTG 
AGGAATTTGC GACGTGTTGC TAGAGAATTG GGTGAAAACA TGAGTGATGA AGAACTTCGA 
GCTATGATAG AAGAATTTGA CAAAGATGGT GATGGAGAAA TAAACCAAGA GGAGTTCATT 
GCTATTATGA CTGGTGACAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CETN3 ( NM_001297765.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001284694.1 Homo sapiens centrin 3 (CETN3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu39408
Clone ID Related Accession (Same CDS sequence)	NM_001297765.1
Accession Version	NM_001297765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	centrin-3 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB998764.1, BM553126.1 and BQ000279.1. On Jul 10, 2014 this sequence version replaced XM_006714521.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM553126.1, SRR1163655.88989.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAGTTTAG CTCTGAGAAG TGAGCTTGTA GTGGACAAAA CAAAGAGGAA AAAAAGAAGA 
GAACTGTCTG AGGAACAGAA ACAAGAAATT AAAGATGCTT TTGAACTATT TGATACAGAC 
AAAGATGAAG CAATAGATTA TCATGAATTA AAGGTGGCAA TGAGAGCCTT GGGGTTTGAT 
GTAAAAAAAG CTGATGTACT GAAGATTCTT AAAGATTATG ACAGAGAAGC CACAGGGAAA 
ATCACCTTTG AAGATTTTAA TGAAGTTGTG ACAGACTGGA TATTGGAAAG AGATCCCCAT 
GAAGAAATAC TCAAGGCATT TAAACTATTT GATGATGATG ATTCAGGTAA AATAAGCTTG 
AGGAATTTGC GACGTGTTGC TAGAGAATTG GGTGAAAACA TGAGTGATGA AGAACTTCGA 
GCTATGATAG AAGAATTTGA CAAAGATGGT GATGGAGAAA TTCTTAAGAA CATACTCTTG 
CTTCCTATTT GGTCTAGATG CCTCAGTCTA AATCGGGAGT TCTTCAGTGA AGTAAACCAA 
GAGGAGTTCA TTGCTATTAT GACTGGTGAC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284694.1
CDS	126..701
Translation	MSLALRSELVVDKTKRKKRRELSEEQKQEIKDAFELFDTDKDEAIDYHELKVAMRALGFDVKKADVLKILKDYDREATGKITFEDFNEVVTDWILERDPHEEILKAFKLFDDDDSGKISLRNLRRVARELGENMSDEELRAMIEEFDKDGDGEILKNILLLPIWSRCLSLNREFFSEVNQEEFIAIMTGDI

Target ORF information:

RefSeq Version	NM_001297765.1
Organism	Homo sapiens(human)
Definition	Homo sapiens centrin 3 (CETN3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297765.1

ORF Insert Sequence:

ATGAGTTTAG CTCTGAGAAG TGAGCTTGTA GTGGACAAAA CAAAGAGGAA AAAAAGAAGA 
GAACTGTCTG AGGAACAGAA ACAAGAAATT AAAGATGCTT TTGAACTATT TGATACAGAC 
AAAGATGAAG CAATAGATTA TCATGAATTA AAGGTGGCAA TGAGAGCCTT GGGGTTTGAT 
GTAAAAAAAG CTGATGTACT GAAGATTCTT AAAGATTATG ACAGAGAAGC CACAGGGAAA 
ATCACCTTTG AAGATTTTAA TGAAGTTGTG ACAGACTGGA TATTGGAAAG AGATCCCCAT 
GAAGAAATAC TCAAGGCATT TAAACTATTT GATGATGATG ATTCAGGTAA AATAAGCTTG 
AGGAATTTGC GACGTGTTGC TAGAGAATTG GGTGAAAACA TGAGTGATGA AGAACTTCGA 
GCTATGATAG AAGAATTTGA CAAAGATGGT GATGGAGAAA TTCTTAAGAA CATACTCTTG 
CTTCCTATTT GGTCTAGATG CCTCAGTCTA AATCGGGAGT TCTTCAGTGA AGTAAACCAA 
GAGGAGTTCA TTGCTATTAT GACTGGTGAC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CETN3 ( NM_001297768.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001284697.1 Homo sapiens centrin 3 (CETN3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu56825
Clone ID Related Accession (Same CDS sequence)	NM_001297768.1
Accession Version	NM_001297768.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	centrin-3 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB998764.1, BU569574.1, BC093793.1 and BQ000279.1. Transcript Variant: This variant (3) has an alternate splice site in the 3' coding region but maintains the reading frame, compared to variant 1. The resulting isoform (3) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU569574.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968 [ECO:0000348] ##Evidence-Data-END##

ATGAGTTTAG CTCTGAGAAG TGAGCTTGTA GTGGACAAAA CAAAGAGGAA AAAAAGAAGA 
GAACTGTCTG AGGAACAGAA ACAAGAAATT AAAGATGCTT TTGAACTATT TGATACAGAC 
AAAGATGAAG CAATAGATTA TCATGAATTA AAGGTGGCAA TGAGAGCCTT GGGGTTTGAT 
GTAAAAAAAG CTGATGTACT GAAGATTCTT AAAGATTATG ACAGAGAAGC CACAGGGAAA 
ATCACCTTTG AAGATTTTAA TGAAGTTGTG ACAGACTGGA TATTGGAAAG AGATCCCCAT 
GAAGAAATAC TCAAGGCATT TAAACTATTT GATGATGATG ATTCAGTTCT TAAGAACATA 
CTCTTGCTTC CTATTTGGTC TAGATGCCTC AGTCTAAATC GGGAGTTCTT CAGTGAAGTA 
AACCAAGAGG AGTTCATTGC TATTATGACT GGTGACATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284697.1
CDS	126..587
Translation	MSLALRSELVVDKTKRKKRRELSEEQKQEIKDAFELFDTDKDEAIDYHELKVAMRALGFDVKKADVLKILKDYDREATGKITFEDFNEVVTDWILERDPHEEILKAFKLFDDDDSVLKNILLLPIWSRCLSLNREFFSEVNQEEFIAIMTGDI

Target ORF information:

RefSeq Version	NM_001297768.1
Organism	Homo sapiens(human)
Definition	Homo sapiens centrin 3 (CETN3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297768.1

ORF Insert Sequence:

ATGAGTTTAG CTCTGAGAAG TGAGCTTGTA GTGGACAAAA CAAAGAGGAA AAAAAGAAGA 
GAACTGTCTG AGGAACAGAA ACAAGAAATT AAAGATGCTT TTGAACTATT TGATACAGAC 
AAAGATGAAG CAATAGATTA TCATGAATTA AAGGTGGCAA TGAGAGCCTT GGGGTTTGAT 
GTAAAAAAAG CTGATGTACT GAAGATTCTT AAAGATTATG ACAGAGAAGC CACAGGGAAA 
ATCACCTTTG AAGATTTTAA TGAAGTTGTG ACAGACTGGA TATTGGAAAG AGATCCCCAT 
GAAGAAATAC TCAAGGCATT TAAACTATTT GATGATGATG ATTCAGTTCT TAAGAACATA 
CTCTTGCTTC CTATTTGGTC TAGATGCCTC AGTCTAAATC GGGAGTTCTT CAGTGAAGTA 
AACCAAGAGG AGTTCATTGC TATTATGACT GGTGACATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface.
Cell163(6)1484-99(2015 Dec)
Gupta GD,Coyaud ?,Gon?alves J,Mojarad BA,Liu Y,Wu Q,Gheiratmand L,Comartin D,Tkach JM,Cheung SW,Bashkurov M,Hasegan M,Knight JD,Lin ZY,Schueler M,Hildebrandt F,Moffat J,Gingras AC,Raught B,Pelletier L

MDM1 is a microtubule-binding protein that negatively regulates centriole duplication.
Molecular biology of the cell26(21)3788-802(2015 Nov)
Van de Mark D,Kong D,Loncarek J,Stearns T

Centrin 3 is an inhibitor of centrosomal Mps1 and antagonizes centrin 2 function.
Molecular biology of the cell26(21)3741-53(2015 Nov)
Sawant DB,Majumder S,Perkins JL,Yang CH,Eyers PA,Fisk HA

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CETN3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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