DDX17 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX17
Entrez Gene ID	10521
Full Name	DEAD-box helicase 17
Synonyms	P72,RH70
General protein information	Preferred Names DEAD-box helicase 17 Names probable ATP-dependent RNA helicase DDX17 DEAD (Asp-Glu-Ala-Asp) box helicase 17 DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 DEAD box protein p72 DEAD box protein p82 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 17 (72kD) RNA-dependent helicase p72
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.1
Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each other than to any other member of the DEAD box family. This gene can encode multiple isoforms due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) start codon. [provided by RefSeq, Apr 2011].
Disorder MIM:	608469

mRNA and Protein(s)

mRNA	Protein	Name
NM_006386.4	NP_006377.2	probable ATP-dependent RNA helicase DDX17 isoform 1
NM_001098504.2	NP_001091974.1	probable ATP-dependent RNA helicase DDX17 isoform 3
NM_006386.5	NP_006377.2	probable ATP-dependent RNA helicase DDX17 isoform 1
NM_001098504.1	NP_001091974.1	probable ATP-dependent RNA helicase DDX17 isoform 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DDX17	XP_003953938.1
	DDX17	XP_001092491.2
Canis lupus familiaris (dog)	DDX17	XP_860668.3
Bos taurus (cattle)	DDX17	NP_001095463.1
Xenopus tropicalis (tropical clawed frog)	ddx17	NP_001016781.1
Homo sapiens (human)	DDX17	NP_006377.2
Caenorhabditis elegans (roundworm)	F58E10.3	NP_001041134.1
Mus musculus (house mouse)	Ddx17	NP_001035277.1
Gallus gallus (chicken)	DDX17	XP_416260.2
Danio rerio (zebrafish)	LOC556764	XP_001923830.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005652	XP_315671.4

Gene Ontology
Bibliography

Related articles in PubMed

Exome sequencing identifies targets in the treatment-resistant ophthalmoplegic subphenotype of myasthenia gravis.
Nel M, Jalali Sefid Dashti M, Gamieldien J, Heckmann JM
Neuromuscular disorders : NMD27(9)816-825(2017 Sep)

DDX17 nucleocytoplasmic shuttling promotes acquired gefitinib resistance in non-small cell lung cancer cells via activation of ?-catenin.
Li K, Mo C, Gong D, Chen Y, Huang Z, Li Y, Zhang J, Huang L, Li Y, Fuller-Pace FV, Lin P, Wei Y
Cancer letters400194-202(2017 Aug)

The miRNA biogenesis factors, p72/DDX17 and KHSRP regulate the protein level of Ago2 in human cells.
Connerty P, Bajan S, Remenyi J, Fuller-Pace FV, Hutvagner G
Biochimica et biophysica acta1859(10)1299-305(2016 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DDX17 contributes to acquired gefitinib resistance through exportin/importin-dependent cytoplasmic shuttling and activation of beta-catenin in non-small lung cancer cells.
Title: DDX17 nucleocytoplasmic shuttling promotes acquired gefitinib resistance in non-small cell lung cancer cells via activation of ?-catenin.

The miRNA biogenesis factors, DDX17 and KHSRP, regulate the protein level of Ago2 in human cells.
Title: The miRNA biogenesis factors, p72/DDX17 and KHSRP regulate the protein level of Ago2 in human cells.

Mutant p53 protein (Mutp53) binds and sequesters RNA helicases p72/82 from microprocessor causing an attenuation of microRNAs (miRNAs) maturation.
Title: Mutant p53 inhibits miRNA biogenesis by interfering with the microprocessor complex.

Overexpression of p72 decreased Beclin1 expression partially by increasing miR-34-5p and miR-5195-3p expression in glioma cells.
Title: Upregulation of p72 Enhances Malignant Migration and Invasion of Glioma Cells by Repressing Beclin1 Expression.

DDX17 is a Sox2-binding protein in estrogen receptor-positive breast cancer; in reporter responsive (RR) cells but not reporter unresponsive (RU) cells, DDX17 enhances the tumorigenic and stem-like features of Sox2 by promoting its binding to its target genes
Title: DDX17 (P72), a Sox2 binding partner, promotes stem-like features conferred by Sox2 in a small cell population in estrogen receptor-positive breast cancer.

The following DDX17 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX17 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22091	NM_001098504.2 Latest version!	Homo sapiens DEAD-box helicase 17 (DDX17), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu12607	NM_006386.5 Latest version!	Homo sapiens DEAD-box helicase 17 (DDX17), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OHu12607	NM_006386.4	Homo sapiens DEAD-box helicase 17 (DDX17), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu22091	NM_001098504.1	Homo sapiens DEAD-box helicase 17 (DDX17), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX17 ( NM_001098504.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001091974.1 Homo sapiens DEAD-box helicase 17 (DDX17), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22091
Clone ID Related Accession (Same CDS sequence)	NM_001098504.2 , NM_001098504.1
Accession Version	NM_001098504.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX17 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP353248.1, AB209595.1, AK024985.1, CB215934.1, Z97056.1 and AL080113.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001098504.1. Transcript Variant: This variant (3) uses a different donor splice site (6 nt downstream) in the penultimate coding exon, but initiates translation from the same non-AUG (CUG) codon as transcript variant 1, resulting in an isoform (3) that is 2 aa longer than isoform 1. Alternate translation from an in-frame, downstream AUG yields a shorter isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.142447.1, SRR1660809.25723.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467150 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11675387 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGCCCACCG GCTTTGTAGC CCCGATTCTC TGTGTTTTGC TCCCGTCTCC GACGAGAGAG 
GCGGCGACGG TGGCGTCTGC GACGGGAGAC AGCGCGTCGG AGCGAGAGAG CGCTGCGCCT 
GCCGCCGCCC CAACAGCGGA GGCGCCGCCG CCATCGGTCG TCACCAGACC GGAGCCGCAG 
GCCCTCCCGA GCCCGGCCAT CCGTGCCCCG CTCCCAGATC TCTATCCTTT TGGGACCATG 
CGCGGAGGAG GCTTTGGGGA CCGGGACCGG GATCGTGACC GTGGAGGATT TGGAGCAAGA 
GGTGGTGGTG GCCTTCCCCC GAAGAAATTT GGTAATCCTG GGGAGCGTTT GCGTAAAAAA 
AAGTGGGATT TGAGTGAGCT CCCCAAGTTT GAGAAAAATT TTTATGTGGA ACATCCGGAA 
GTAGCAAGGC TGACACCATA TGAGGTTGAT GAGCTACGCC GAAAGAAGGA GATTACAGTG 
AGGGGGGGAG ATGTTTGTCC TAAACCCGTG TTTGCCTTCC ATCATGCTAA CTTCCCACAA 
TATGTAATGG ATGTGTTGAT GGATCAGCAC TTTACAGAAC CAACTCCAAT TCAGTGCCAG 
GGATTTCCGT TGGCTCTTAG TGGCCGGGAT ATGGTGGGCA TTGCTCAGAC TGGCTCTGGG 
AAGACGTTGG CGTATCTCCT GCCTGCAATT GTTCATATTA ACCACCAGCC ATACTTGGAA 
AGGGGAGATG GCCCAATCTG TCTAGTTCTG GCTCCTACCA GAGAGCTTGC CCAGCAAGTA 
CAGCAGGTGG CCGATGACTA TGGCAAATGT TCTAGATTGA AGAGTACTTG TATTTATGGA 
GGTGCTCCTA AAGGTCCCCA GATTCGAGAC TTGGAAAGAG GTGTTGAGAT CTGCATAGCC 
ACTCCTGGAC GTCTGATAGA TTTCCTGGAG TCAGGAAAGA CAAATCTTCG CCGATGTACT 
TACCTTGTAT TGGACGAAGC TGACAGAATG CTTGATATGG GGTTTGAACC CCAGATCCGT 
AAAATTGTTG ACCAAATCAG GCCTGATAGG CAGACACTGA TGTGGAGTGC AACCTGGCCA 
AAAGAAGTAA GACAGCTTGC AGAGGATTTC CTTCGTGATT ACACCCAGAT CAACGTAGGC 
AATCTGGAGT TGAGTGCCAA CCACAACATC CTCCAGATAG TGGATGTCTG CATGGAAAGT 
GAAAAAGACC ACAAGTTGAT CCAACTAATG GAAGAAATAA TGGCTGAAAA GGAAAACAAA 
ACAATAATAT TTGTGGAGAC AAAGAGACGC TGTGATGATC TGACTCGAAG GATGCGCAGA 
GATGGTTGGC CAGCTATGTG TATCCATGGA GACAAGAGTC AACCAGAAAG AGATTGGGTA 
CTTAATGAGT TCCGTTCTGG AAAGGCACCC ATCCTTATTG CTACAGATGT AGCCTCCCGT 
GGGCTAGATG TGGAAGATGT CAAGTTTGTG ATCAACTATG ACTATCCAAA CAGCTCAGAG 
GATTATGTGC ACCGTATTGG CCGAACAGCC CGTAGCACCA ACAAGGGTAC CGCCTATACC 
TTCTTCACCC CAGGGAACCT AAAACAGGCC AGAGAGCTTA TCAAAGTGCT GGAAGAGGCC 
AATCAGGCTA TCAATCCAAA ACTGATGCAG CTTGTGGACC ACAGAGGAGG CGGCGGAGGC 
GGGGGTAAGG GTGGTCGTTC TCGTTACCGG ACCACTTCTT CAGCCAACAA TCCCAATCTG 
ATGTATCAGG ATGAGTGTGA CCGAAGGCTT CGAGGAGTCA AGGATGGTGG CCGGAGAGAC 
TCTGCAAGCT ATCGGGATCG TAGTGAAACC GATAGAGCTG GTTATGCTAA TGGCAGTGGC 
TATGGAAGTC CAAATTCTGC CTTTGGAGCA CAAGCAGGCC AATACACCTA TGGTCAAGGC 
ACCTATGGGG CAGCTGCTTA TGGCACCAGT AGCTATACAG CTCAAGAATA TGGTGCTGGC 
ACTTATGGAG CTAGTAGCAC CACCTCAACT GGGAGAAGTT CACAGAGCTC TAGCCAGCAG 
TTTAGTGGGA TAGGCCGGTC TGGGCAGCAG CCACAGCCAC TGATGTCACA ACAGTTTGCA 
CAGCCTCCGG GAGCTACCAA TATGATAGGT TACATGGGGC AGACTGCCTA CCAATACCCT 
CCTCCTCCTC CCCCTCCTCC TCCTTCACGT AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091974.1
CDS	75..2270
Translation	LPTGFVAPILCVLLPSPTREAATVASATGDSASERESAAPAAAPTAEAPPPSVVTRPEPQALPSPAIRAPLPDLYPFGTMRGGGFGDRDRDRDRGGFGARGGGGLPPKKFGNPGERLRKKKWDLSELPKFEKNFYVEHPEVARLTPYEVDELRRKKEITVRGGDVCPKPVFAFHHANFPQYVMDVLMDQHFTEPTPIQCQGFPLALSGRDMVGIAQTGSGKTLAYLLPAIVHINHQPYLERGDGPICLVLAPTRELAQQVQQVADDYGKCSRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLESGKTNLRRCTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLRDYTQINVGNLELSANHNILQIVDVCMESEKDHKLIQLMEEIMAEKENKTIIFVETKRRCDDLTRRMRRDGWPAMCIHGDKSQPERDWVLNEFRSGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYVHRIGRTARSTNKGTAYTFFTPGNLKQARELIKVLEEANQAINPKLMQLVDHRGGGGGGGKGGRSRYRTTSSANNPNLMYQDECDRRLRGVKDGGRRDSASYRDRSETDRAGYANGSGYGSPNSAFGAQAGQYTYGQGTYGAAAYGTSSYTAQEYGAGTYGASSTTSTGRSSQSSSQQFSGIGRSGQQPQPLMSQQFAQPPGATNMIGYMGQTAYQYPPPPPPPPPSRK

Target ORF information:

RefSeq Version	NM_001098504.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 17 (DDX17), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098504.2

ORF Insert Sequence:

CTGCCCACCG GCTTTGTAGC CCCGATTCTC TGTGTTTTGC TCCCGTCTCC GACGAGAGAG 
GCGGCGACGG TGGCGTCTGC GACGGGAGAC AGCGCGTCGG AGCGAGAGAG CGCTGCGCCT 
GCCGCCGCCC CAACAGCGGA GGCGCCGCCG CCATCGGTCG TCACCAGACC GGAGCCGCAG 
GCCCTCCCGA GCCCGGCCAT CCGTGCCCCG CTCCCAGATC TCTATCCTTT TGGGACCATG 
CGCGGAGGAG GCTTTGGGGA CCGGGACCGG GATCGTGACC GTGGAGGATT TGGAGCAAGA 
GGTGGTGGTG GCCTTCCCCC GAAGAAATTT GGTAATCCTG GGGAGCGTTT GCGTAAAAAA 
AAGTGGGATT TGAGTGAGCT CCCCAAGTTT GAGAAAAATT TTTATGTGGA ACATCCGGAA 
GTAGCAAGGC TGACACCATA TGAGGTTGAT GAGCTACGCC GAAAGAAGGA GATTACAGTG 
AGGGGGGGAG ATGTTTGTCC TAAACCCGTG TTTGCCTTCC ATCATGCTAA CTTCCCACAA 
TATGTAATGG ATGTGTTGAT GGATCAGCAC TTTACAGAAC CAACTCCAAT TCAGTGCCAG 
GGATTTCCGT TGGCTCTTAG TGGCCGGGAT ATGGTGGGCA TTGCTCAGAC TGGCTCTGGG 
AAGACGTTGG CGTATCTCCT GCCTGCAATT GTTCATATTA ACCACCAGCC ATACTTGGAA 
AGGGGAGATG GCCCAATCTG TCTAGTTCTG GCTCCTACCA GAGAGCTTGC CCAGCAAGTA 
CAGCAGGTGG CCGATGACTA TGGCAAATGT TCTAGATTGA AGAGTACTTG TATTTATGGA 
GGTGCTCCTA AAGGTCCCCA GATTCGAGAC TTGGAAAGAG GTGTTGAGAT CTGCATAGCC 
ACTCCTGGAC GTCTGATAGA TTTCCTGGAG TCAGGAAAGA CAAATCTTCG CCGATGTACT 
TACCTTGTAT TGGACGAAGC TGACAGAATG CTTGATATGG GGTTTGAACC CCAGATCCGT 
AAAATTGTTG ACCAAATCAG GCCTGATAGG CAGACACTGA TGTGGAGTGC AACCTGGCCA 
AAAGAAGTAA GACAGCTTGC AGAGGATTTC CTTCGTGATT ACACCCAGAT CAACGTAGGC 
AATCTGGAGT TGAGTGCCAA CCACAACATC CTCCAGATAG TGGATGTCTG CATGGAAAGT 
GAAAAAGACC ACAAGTTGAT CCAACTAATG GAAGAAATAA TGGCTGAAAA GGAAAACAAA 
ACAATAATAT TTGTGGAGAC AAAGAGACGC TGTGATGATC TGACTCGAAG GATGCGCAGA 
GATGGTTGGC CAGCTATGTG TATCCATGGA GACAAGAGTC AACCAGAAAG AGATTGGGTA 
CTTAATGAGT TCCGTTCTGG AAAGGCACCC ATCCTTATTG CTACAGATGT AGCCTCCCGT 
GGGCTAGATG TGGAAGATGT CAAGTTTGTG ATCAACTATG ACTATCCAAA CAGCTCAGAG 
GATTATGTGC ACCGTATTGG CCGAACAGCC CGTAGCACCA ACAAGGGTAC CGCCTATACC 
TTCTTCACCC CAGGGAACCT AAAACAGGCC AGAGAGCTTA TCAAAGTGCT GGAAGAGGCC 
AATCAGGCTA TCAATCCAAA ACTGATGCAG CTTGTGGACC ACAGAGGAGG CGGCGGAGGC 
GGGGGTAAGG GTGGTCGTTC TCGTTACCGG ACCACTTCTT CAGCCAACAA TCCCAATCTG 
ATGTATCAGG ATGAGTGTGA CCGAAGGCTT CGAGGAGTCA AGGATGGTGG CCGGAGAGAC 
TCTGCAAGCT ATCGGGATCG TAGTGAAACC GATAGAGCTG GTTATGCTAA TGGCAGTGGC 
TATGGAAGTC CAAATTCTGC CTTTGGAGCA CAAGCAGGCC AATACACCTA TGGTCAAGGC 
ACCTATGGGG CAGCTGCTTA TGGCACCAGT AGCTATACAG CTCAAGAATA TGGTGCTGGC 
ACTTATGGAG CTAGTAGCAC CACCTCAACT GGGAGAAGTT CACAGAGCTC TAGCCAGCAG 
TTTAGTGGGA TAGGCCGGTC TGGGCAGCAG CCACAGCCAC TGATGTCACA ACAGTTTGCA 
CAGCCTCCGG GAGCTACCAA TATGATAGGT TACATGGGGC AGACTGCCTA CCAATACCCT 
CCTCCTCCTC CCCCTCCTCC TCCTTCACGT AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX17 ( NM_001098504.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001091974.1 Homo sapiens DEAD-box helicase 17 (DDX17), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22091
Clone ID Related Accession (Same CDS sequence)	NM_001098504.2 , NM_001098504.1
Accession Version	NM_001098504.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX17 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP353248.1, AB209595.1, AK024985.1, CB215934.1, Z97056.1 and AL080113.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) uses a different donor splice site (6 nt downstream) in the penultimate coding exon, but initiates translation from the same non-AUG (CUG) codon as transcript variant 1, resulting in an isoform (3) that is 2 aa longer than isoform 1. Alternate translation from an in-frame, downstream AUG yields a shorter isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.169295.1, SRR1163657.156766.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467150 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11675387 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGCCCACCG GCTTTGTAGC CCCGATTCTC TGTGTTTTGC TCCCGTCTCC GACGAGAGAG 
GCGGCGACGG TGGCGTCTGC GACGGGAGAC AGCGCGTCGG AGCGAGAGAG CGCTGCGCCT 
GCCGCCGCCC CAACAGCGGA GGCGCCGCCG CCATCGGTCG TCACCAGACC GGAGCCGCAG 
GCCCTCCCGA GCCCGGCCAT CCGTGCCCCG CTCCCAGATC TCTATCCTTT TGGGACCATG 
CGCGGAGGAG GCTTTGGGGA CCGGGACCGG GATCGTGACC GTGGAGGATT TGGAGCAAGA 
GGTGGTGGTG GCCTTCCCCC GAAGAAATTT GGTAATCCTG GGGAGCGTTT GCGTAAAAAA 
AAGTGGGATT TGAGTGAGCT CCCCAAGTTT GAGAAAAATT TTTATGTGGA ACATCCGGAA 
GTAGCAAGGC TGACACCATA TGAGGTTGAT GAGCTACGCC GAAAGAAGGA GATTACAGTG 
AGGGGGGGAG ATGTTTGTCC TAAACCCGTG TTTGCCTTCC ATCATGCTAA CTTCCCACAA 
TATGTAATGG ATGTGTTGAT GGATCAGCAC TTTACAGAAC CAACTCCAAT TCAGTGCCAG 
GGATTTCCGT TGGCTCTTAG TGGCCGGGAT ATGGTGGGCA TTGCTCAGAC TGGCTCTGGG 
AAGACGTTGG CGTATCTCCT GCCTGCAATT GTTCATATTA ACCACCAGCC ATACTTGGAA 
AGGGGAGATG GCCCAATCTG TCTAGTTCTG GCTCCTACCA GAGAGCTTGC CCAGCAAGTA 
CAGCAGGTGG CCGATGACTA TGGCAAATGT TCTAGATTGA AGAGTACTTG TATTTATGGA 
GGTGCTCCTA AAGGTCCCCA GATTCGAGAC TTGGAAAGAG GTGTTGAGAT CTGCATAGCC 
ACTCCTGGAC GTCTGATAGA TTTCCTGGAG TCAGGAAAGA CAAATCTTCG CCGATGTACT 
TACCTTGTAT TGGACGAAGC TGACAGAATG CTTGATATGG GGTTTGAACC CCAGATCCGT 
AAAATTGTTG ACCAAATCAG GCCTGATAGG CAGACACTGA TGTGGAGTGC AACCTGGCCA 
AAAGAAGTAA GACAGCTTGC AGAGGATTTC CTTCGTGATT ACACCCAGAT CAACGTAGGC 
AATCTGGAGT TGAGTGCCAA CCACAACATC CTCCAGATAG TGGATGTCTG CATGGAAAGT 
GAAAAAGACC ACAAGTTGAT CCAACTAATG GAAGAAATAA TGGCTGAAAA GGAAAACAAA 
ACAATAATAT TTGTGGAGAC AAAGAGACGC TGTGATGATC TGACTCGAAG GATGCGCAGA 
GATGGTTGGC CAGCTATGTG TATCCATGGA GACAAGAGTC AACCAGAAAG AGATTGGGTA 
CTTAATGAGT TCCGTTCTGG AAAGGCACCC ATCCTTATTG CTACAGATGT AGCCTCCCGT 
GGGCTAGATG TGGAAGATGT CAAGTTTGTG ATCAACTATG ACTATCCAAA CAGCTCAGAG 
GATTATGTGC ACCGTATTGG CCGAACAGCC CGTAGCACCA ACAAGGGTAC CGCCTATACC 
TTCTTCACCC CAGGGAACCT AAAACAGGCC AGAGAGCTTA TCAAAGTGCT GGAAGAGGCC 
AATCAGGCTA TCAATCCAAA ACTGATGCAG CTTGTGGACC ACAGAGGAGG CGGCGGAGGC 
GGGGGTAAGG GTGGTCGTTC TCGTTACCGG ACCACTTCTT CAGCCAACAA TCCCAATCTG 
ATGTATCAGG ATGAGTGTGA CCGAAGGCTT CGAGGAGTCA AGGATGGTGG CCGGAGAGAC 
TCTGCAAGCT ATCGGGATCG TAGTGAAACC GATAGAGCTG GTTATGCTAA TGGCAGTGGC 
TATGGAAGTC CAAATTCTGC CTTTGGAGCA CAAGCAGGCC AATACACCTA TGGTCAAGGC 
ACCTATGGGG CAGCTGCTTA TGGCACCAGT AGCTATACAG CTCAAGAATA TGGTGCTGGC 
ACTTATGGAG CTAGTAGCAC CACCTCAACT GGGAGAAGTT CACAGAGCTC TAGCCAGCAG 
TTTAGTGGGA TAGGCCGGTC TGGGCAGCAG CCACAGCCAC TGATGTCACA ACAGTTTGCA 
CAGCCTCCGG GAGCTACCAA TATGATAGGT TACATGGGGC AGACTGCCTA CCAATACCCT 
CCTCCTCCTC CCCCTCCTCC TCCTTCACGT AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091974.1
CDS	104..2299
Translation	LPTGFVAPILCVLLPSPTREAATVASATGDSASERESAAPAAAPTAEAPPPSVVTRPEPQALPSPAIRAPLPDLYPFGTMRGGGFGDRDRDRDRGGFGARGGGGLPPKKFGNPGERLRKKKWDLSELPKFEKNFYVEHPEVARLTPYEVDELRRKKEITVRGGDVCPKPVFAFHHANFPQYVMDVLMDQHFTEPTPIQCQGFPLALSGRDMVGIAQTGSGKTLAYLLPAIVHINHQPYLERGDGPICLVLAPTRELAQQVQQVADDYGKCSRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLESGKTNLRRCTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLRDYTQINVGNLELSANHNILQIVDVCMESEKDHKLIQLMEEIMAEKENKTIIFVETKRRCDDLTRRMRRDGWPAMCIHGDKSQPERDWVLNEFRSGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYVHRIGRTARSTNKGTAYTFFTPGNLKQARELIKVLEEANQAINPKLMQLVDHRGGGGGGGKGGRSRYRTTSSANNPNLMYQDECDRRLRGVKDGGRRDSASYRDRSETDRAGYANGSGYGSPNSAFGAQAGQYTYGQGTYGAAAYGTSSYTAQEYGAGTYGASSTTSTGRSSQSSSQQFSGIGRSGQQPQPLMSQQFAQPPGATNMIGYMGQTAYQYPPPPPPPPPSRK

Target ORF information:

RefSeq Version	NM_001098504.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 17 (DDX17), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098504.1

ORF Insert Sequence:

CTGCCCACCG GCTTTGTAGC CCCGATTCTC TGTGTTTTGC TCCCGTCTCC GACGAGAGAG 
GCGGCGACGG TGGCGTCTGC GACGGGAGAC AGCGCGTCGG AGCGAGAGAG CGCTGCGCCT 
GCCGCCGCCC CAACAGCGGA GGCGCCGCCG CCATCGGTCG TCACCAGACC GGAGCCGCAG 
GCCCTCCCGA GCCCGGCCAT CCGTGCCCCG CTCCCAGATC TCTATCCTTT TGGGACCATG 
CGCGGAGGAG GCTTTGGGGA CCGGGACCGG GATCGTGACC GTGGAGGATT TGGAGCAAGA 
GGTGGTGGTG GCCTTCCCCC GAAGAAATTT GGTAATCCTG GGGAGCGTTT GCGTAAAAAA 
AAGTGGGATT TGAGTGAGCT CCCCAAGTTT GAGAAAAATT TTTATGTGGA ACATCCGGAA 
GTAGCAAGGC TGACACCATA TGAGGTTGAT GAGCTACGCC GAAAGAAGGA GATTACAGTG 
AGGGGGGGAG ATGTTTGTCC TAAACCCGTG TTTGCCTTCC ATCATGCTAA CTTCCCACAA 
TATGTAATGG ATGTGTTGAT GGATCAGCAC TTTACAGAAC CAACTCCAAT TCAGTGCCAG 
GGATTTCCGT TGGCTCTTAG TGGCCGGGAT ATGGTGGGCA TTGCTCAGAC TGGCTCTGGG 
AAGACGTTGG CGTATCTCCT GCCTGCAATT GTTCATATTA ACCACCAGCC ATACTTGGAA 
AGGGGAGATG GCCCAATCTG TCTAGTTCTG GCTCCTACCA GAGAGCTTGC CCAGCAAGTA 
CAGCAGGTGG CCGATGACTA TGGCAAATGT TCTAGATTGA AGAGTACTTG TATTTATGGA 
GGTGCTCCTA AAGGTCCCCA GATTCGAGAC TTGGAAAGAG GTGTTGAGAT CTGCATAGCC 
ACTCCTGGAC GTCTGATAGA TTTCCTGGAG TCAGGAAAGA CAAATCTTCG CCGATGTACT 
TACCTTGTAT TGGACGAAGC TGACAGAATG CTTGATATGG GGTTTGAACC CCAGATCCGT 
AAAATTGTTG ACCAAATCAG GCCTGATAGG CAGACACTGA TGTGGAGTGC AACCTGGCCA 
AAAGAAGTAA GACAGCTTGC AGAGGATTTC CTTCGTGATT ACACCCAGAT CAACGTAGGC 
AATCTGGAGT TGAGTGCCAA CCACAACATC CTCCAGATAG TGGATGTCTG CATGGAAAGT 
GAAAAAGACC ACAAGTTGAT CCAACTAATG GAAGAAATAA TGGCTGAAAA GGAAAACAAA 
ACAATAATAT TTGTGGAGAC AAAGAGACGC TGTGATGATC TGACTCGAAG GATGCGCAGA 
GATGGTTGGC CAGCTATGTG TATCCATGGA GACAAGAGTC AACCAGAAAG AGATTGGGTA 
CTTAATGAGT TCCGTTCTGG AAAGGCACCC ATCCTTATTG CTACAGATGT AGCCTCCCGT 
GGGCTAGATG TGGAAGATGT CAAGTTTGTG ATCAACTATG ACTATCCAAA CAGCTCAGAG 
GATTATGTGC ACCGTATTGG CCGAACAGCC CGTAGCACCA ACAAGGGTAC CGCCTATACC 
TTCTTCACCC CAGGGAACCT AAAACAGGCC AGAGAGCTTA TCAAAGTGCT GGAAGAGGCC 
AATCAGGCTA TCAATCCAAA ACTGATGCAG CTTGTGGACC ACAGAGGAGG CGGCGGAGGC 
GGGGGTAAGG GTGGTCGTTC TCGTTACCGG ACCACTTCTT CAGCCAACAA TCCCAATCTG 
ATGTATCAGG ATGAGTGTGA CCGAAGGCTT CGAGGAGTCA AGGATGGTGG CCGGAGAGAC 
TCTGCAAGCT ATCGGGATCG TAGTGAAACC GATAGAGCTG GTTATGCTAA TGGCAGTGGC 
TATGGAAGTC CAAATTCTGC CTTTGGAGCA CAAGCAGGCC AATACACCTA TGGTCAAGGC 
ACCTATGGGG CAGCTGCTTA TGGCACCAGT AGCTATACAG CTCAAGAATA TGGTGCTGGC 
ACTTATGGAG CTAGTAGCAC CACCTCAACT GGGAGAAGTT CACAGAGCTC TAGCCAGCAG 
TTTAGTGGGA TAGGCCGGTC TGGGCAGCAG CCACAGCCAC TGATGTCACA ACAGTTTGCA 
CAGCCTCCGG GAGCTACCAA TATGATAGGT TACATGGGGC AGACTGCCTA CCAATACCCT 
CCTCCTCCTC CCCCTCCTCC TCCTTCACGT AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX17 ( NM_006386.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006377.2 Homo sapiens DEAD-box helicase 17 (DDX17), transcript variant 1, mRNA.

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CloneID	OHu12607
Clone ID Related Accession (Same CDS sequence)	NM_006386.4 , NM_006386.5
Accession Version	NM_006386.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-26
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX17 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP353248.1, AK024985.1, CB215934.1, Z97056.1 and AL080113.1. On Jun 6, 2007 this sequence version replaced NM_006386.3. Transcript Variant: This variant (1) encodes isoform 1 (also known as p82) through the use of a non-AUG (CUG) translation initiation codon. Alternate translation from an in-frame, downstream AUG results in a shorter isoform (known as p72). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK024985.1, AK074224.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467150 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11675387 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGCCCACCG GCTTTGTAGC CCCGATTCTC TGTGTTTTGC TCCCGTCTCC GACGAGAGAG 
GCGGCGACGG TGGCGTCTGC GACGGGAGAC AGCGCGTCGG AGCGAGAGAG CGCTGCGCCT 
GCCGCCGCCC CAACAGCGGA GGCGCCGCCG CCATCGGTCG TCACCAGACC GGAGCCGCAG 
GCCCTCCCGA GCCCGGCCAT CCGTGCCCCG CTCCCAGATC TCTATCCTTT TGGGACCATG 
CGCGGAGGAG GCTTTGGGGA CCGGGACCGG GATCGTGACC GTGGAGGATT TGGAGCAAGA 
GGTGGTGGTG GCCTTCCCCC GAAGAAATTT GGTAATCCTG GGGAGCGTTT GCGTAAAAAA 
AAGTGGGATT TGAGTGAGCT CCCCAAGTTT GAGAAAAATT TTTATGTGGA ACATCCGGAA 
GTAGCAAGGC TGACACCATA TGAGGTTGAT GAGCTACGCC GAAAGAAGGA GATTACAGTG 
AGGGGGGGAG ATGTTTGTCC TAAACCCGTG TTTGCCTTCC ATCATGCTAA CTTCCCACAA 
TATGTAATGG ATGTGTTGAT GGATCAGCAC TTTACAGAAC CAACTCCAAT TCAGTGCCAG 
GGATTTCCGT TGGCTCTTAG TGGCCGGGAT ATGGTGGGCA TTGCTCAGAC TGGCTCTGGG 
AAGACGTTGG CGTATCTCCT GCCTGCAATT GTTCATATTA ACCACCAGCC ATACTTGGAA 
AGGGGAGATG GCCCAATCTG TCTAGTTCTG GCTCCTACCA GAGAGCTTGC CCAGCAAGTA 
CAGCAGGTGG CCGATGACTA TGGCAAATGT TCTAGATTGA AGAGTACTTG TATTTATGGA 
GGTGCTCCTA AAGGTCCCCA GATTCGAGAC TTGGAAAGAG GTGTTGAGAT CTGCATAGCC 
ACTCCTGGAC GTCTGATAGA TTTCCTGGAG TCAGGAAAGA CAAATCTTCG CCGATGTACT 
TACCTTGTAT TGGACGAAGC TGACAGAATG CTTGATATGG GGTTTGAACC CCAGATCCGT 
AAAATTGTTG ACCAAATCAG GCCTGATAGG CAGACACTGA TGTGGAGTGC AACCTGGCCA 
AAAGAAGTAA GACAGCTTGC AGAGGATTTC CTTCGTGATT ACACCCAGAT CAACGTAGGC 
AATCTGGAGT TGAGTGCCAA CCACAACATC CTCCAGATAG TGGATGTCTG CATGGAAAGT 
GAAAAAGACC ACAAGTTGAT CCAACTAATG GAAGAAATAA TGGCTGAAAA GGAAAACAAA 
ACAATAATAT TTGTGGAGAC AAAGAGACGC TGTGATGATC TGACTCGAAG GATGCGCAGA 
GATGGTTGGC CAGCTATGTG TATCCATGGA GACAAGAGTC AACCAGAAAG AGATTGGGTA 
CTTAATGAGT TCCGTTCTGG AAAGGCACCC ATCCTTATTG CTACAGATGT AGCCTCCCGT 
GGGCTAGATG TGGAAGATGT CAAGTTTGTG ATCAACTATG ACTATCCAAA CAGCTCAGAG 
GATTATGTGC ACCGTATTGG CCGAACAGCC CGTAGCACCA ACAAGGGTAC CGCCTATACC 
TTCTTCACCC CAGGGAACCT AAAACAGGCC AGAGAGCTTA TCAAAGTGCT GGAAGAGGCC 
AATCAGGCTA TCAATCCAAA ACTGATGCAG CTTGTGGACC ACAGAGGAGG CGGCGGAGGC 
GGGGGTGGTC GTTCTCGTTA CCGGACCACT TCTTCAGCCA ACAATCCCAA TCTGATGTAT 
CAGGATGAGT GTGACCGAAG GCTTCGAGGA GTCAAGGATG GTGGCCGGAG AGACTCTGCA 
AGCTATCGGG ATCGTAGTGA AACCGATAGA GCTGGTTATG CTAATGGCAG TGGCTATGGA 
AGTCCAAATT CTGCCTTTGG AGCACAAGCA GGCCAATACA CCTATGGTCA AGGCACCTAT 
GGGGCAGCTG CTTATGGCAC CAGTAGCTAT ACAGCTCAAG AATATGGTGC TGGCACTTAT 
GGAGCTAGTA GCACCACCTC AACTGGGAGA AGTTCACAGA GCTCTAGCCA GCAGTTTAGT 
GGGATAGGCC GGTCTGGGCA GCAGCCACAG CCACTGATGT CACAACAGTT TGCACAGCCT 
CCGGGAGCTA CCAATATGAT AGGTTACATG GGGCAGACTG CCTACCAATA CCCTCCTCCT 
CCTCCCCCTC CTCCTCCTTC ACGTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006377.2
CDS	104..2293
Translation	LPTGFVAPILCVLLPSPTREAATVASATGDSASERESAAPAAAPTAEAPPPSVVTRPEPQALPSPAIRAPLPDLYPFGTMRGGGFGDRDRDRDRGGFGARGGGGLPPKKFGNPGERLRKKKWDLSELPKFEKNFYVEHPEVARLTPYEVDELRRKKEITVRGGDVCPKPVFAFHHANFPQYVMDVLMDQHFTEPTPIQCQGFPLALSGRDMVGIAQTGSGKTLAYLLPAIVHINHQPYLERGDGPICLVLAPTRELAQQVQQVADDYGKCSRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLESGKTNLRRCTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLRDYTQINVGNLELSANHNILQIVDVCMESEKDHKLIQLMEEIMAEKENKTIIFVETKRRCDDLTRRMRRDGWPAMCIHGDKSQPERDWVLNEFRSGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYVHRIGRTARSTNKGTAYTFFTPGNLKQARELIKVLEEANQAINPKLMQLVDHRGGGGGGGGRSRYRTTSSANNPNLMYQDECDRRLRGVKDGGRRDSASYRDRSETDRAGYANGSGYGSPNSAFGAQAGQYTYGQGTYGAAAYGTSSYTAQEYGAGTYGASSTTSTGRSSQSSSQQFSGIGRSGQQPQPLMSQQFAQPPGATNMIGYMGQTAYQYPPPPPPPPPSRK

Target ORF information:

RefSeq Version	NM_006386.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 17 (DDX17), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006386.4

ORF Insert Sequence:

CTGCCCACCG GCTTTGTAGC CCCGATTCTC TGTGTTTTGC TCCCGTCTCC GACGAGAGAG 
GCGGCGACGG TGGCGTCTGC GACGGGAGAC AGCGCGTCGG AGCGAGAGAG CGCTGCGCCT 
GCCGCCGCCC CAACAGCGGA GGCGCCGCCG CCATCGGTCG TCACCAGACC GGAGCCGCAG 
GCCCTCCCGA GCCCGGCCAT CCGTGCCCCG CTCCCAGATC TCTATCCTTT TGGGACCATG 
CGCGGAGGAG GCTTTGGGGA CCGGGACCGG GATCGTGACC GTGGAGGATT TGGAGCAAGA 
GGTGGTGGTG GCCTTCCCCC GAAGAAATTT GGTAATCCTG GGGAGCGTTT GCGTAAAAAA 
AAGTGGGATT TGAGTGAGCT CCCCAAGTTT GAGAAAAATT TTTATGTGGA ACATCCGGAA 
GTAGCAAGGC TGACACCATA TGAGGTTGAT GAGCTACGCC GAAAGAAGGA GATTACAGTG 
AGGGGGGGAG ATGTTTGTCC TAAACCCGTG TTTGCCTTCC ATCATGCTAA CTTCCCACAA 
TATGTAATGG ATGTGTTGAT GGATCAGCAC TTTACAGAAC CAACTCCAAT TCAGTGCCAG 
GGATTTCCGT TGGCTCTTAG TGGCCGGGAT ATGGTGGGCA TTGCTCAGAC TGGCTCTGGG 
AAGACGTTGG CGTATCTCCT GCCTGCAATT GTTCATATTA ACCACCAGCC ATACTTGGAA 
AGGGGAGATG GCCCAATCTG TCTAGTTCTG GCTCCTACCA GAGAGCTTGC CCAGCAAGTA 
CAGCAGGTGG CCGATGACTA TGGCAAATGT TCTAGATTGA AGAGTACTTG TATTTATGGA 
GGTGCTCCTA AAGGTCCCCA GATTCGAGAC TTGGAAAGAG GTGTTGAGAT CTGCATAGCC 
ACTCCTGGAC GTCTGATAGA TTTCCTGGAG TCAGGAAAGA CAAATCTTCG CCGATGTACT 
TACCTTGTAT TGGACGAAGC TGACAGAATG CTTGATATGG GGTTTGAACC CCAGATCCGT 
AAAATTGTTG ACCAAATCAG GCCTGATAGG CAGACACTGA TGTGGAGTGC AACCTGGCCA 
AAAGAAGTAA GACAGCTTGC AGAGGATTTC CTTCGTGATT ACACCCAGAT CAACGTAGGC 
AATCTGGAGT TGAGTGCCAA CCACAACATC CTCCAGATAG TGGATGTCTG CATGGAAAGT 
GAAAAAGACC ACAAGTTGAT CCAACTAATG GAAGAAATAA TGGCTGAAAA GGAAAACAAA 
ACAATAATAT TTGTGGAGAC AAAGAGACGC TGTGATGATC TGACTCGAAG GATGCGCAGA 
GATGGTTGGC CAGCTATGTG TATCCATGGA GACAAGAGTC AACCAGAAAG AGATTGGGTA 
CTTAATGAGT TCCGTTCTGG AAAGGCACCC ATCCTTATTG CTACAGATGT AGCCTCCCGT 
GGGCTAGATG TGGAAGATGT CAAGTTTGTG ATCAACTATG ACTATCCAAA CAGCTCAGAG 
GATTATGTGC ACCGTATTGG CCGAACAGCC CGTAGCACCA ACAAGGGTAC CGCCTATACC 
TTCTTCACCC CAGGGAACCT AAAACAGGCC AGAGAGCTTA TCAAAGTGCT GGAAGAGGCC 
AATCAGGCTA TCAATCCAAA ACTGATGCAG CTTGTGGACC ACAGAGGAGG CGGCGGAGGC 
GGGGGTGGTC GTTCTCGTTA CCGGACCACT TCTTCAGCCA ACAATCCCAA TCTGATGTAT 
CAGGATGAGT GTGACCGAAG GCTTCGAGGA GTCAAGGATG GTGGCCGGAG AGACTCTGCA 
AGCTATCGGG ATCGTAGTGA AACCGATAGA GCTGGTTATG CTAATGGCAG TGGCTATGGA 
AGTCCAAATT CTGCCTTTGG AGCACAAGCA GGCCAATACA CCTATGGTCA AGGCACCTAT 
GGGGCAGCTG CTTATGGCAC CAGTAGCTAT ACAGCTCAAG AATATGGTGC TGGCACTTAT 
GGAGCTAGTA GCACCACCTC AACTGGGAGA AGTTCACAGA GCTCTAGCCA GCAGTTTAGT 
GGGATAGGCC GGTCTGGGCA GCAGCCACAG CCACTGATGT CACAACAGTT TGCACAGCCT 
CCGGGAGCTA CCAATATGAT AGGTTACATG GGGCAGACTG CCTACCAATA CCCTCCTCCT 
CCTCCCCCTC CTCCTCCTTC ACGTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX17 ( NM_006386.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_006377.2 Homo sapiens DEAD-box helicase 17 (DDX17), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu12607
Clone ID Related Accession (Same CDS sequence)	NM_006386.4 , NM_006386.5
Accession Version	NM_006386.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX17 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP353248.1, AK024985.1, CB215934.1, Z97056.1 and AL080113.1. On Nov 22, 2018 this sequence version replaced NM_006386.4. Transcript Variant: This variant (1) encodes isoform 1 (also known as p82) through the use of a non-AUG (CUG) translation initiation codon. Alternate translation from an in-frame, downstream AUG results in a shorter isoform (known as p72). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.25535.1, AK024985.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467150 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 11675387 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGCCCACCG GCTTTGTAGC CCCGATTCTC TGTGTTTTGC TCCCGTCTCC GACGAGAGAG 
GCGGCGACGG TGGCGTCTGC GACGGGAGAC AGCGCGTCGG AGCGAGAGAG CGCTGCGCCT 
GCCGCCGCCC CAACAGCGGA GGCGCCGCCG CCATCGGTCG TCACCAGACC GGAGCCGCAG 
GCCCTCCCGA GCCCGGCCAT CCGTGCCCCG CTCCCAGATC TCTATCCTTT TGGGACCATG 
CGCGGAGGAG GCTTTGGGGA CCGGGACCGG GATCGTGACC GTGGAGGATT TGGAGCAAGA 
GGTGGTGGTG GCCTTCCCCC GAAGAAATTT GGTAATCCTG GGGAGCGTTT GCGTAAAAAA 
AAGTGGGATT TGAGTGAGCT CCCCAAGTTT GAGAAAAATT TTTATGTGGA ACATCCGGAA 
GTAGCAAGGC TGACACCATA TGAGGTTGAT GAGCTACGCC GAAAGAAGGA GATTACAGTG 
AGGGGGGGAG ATGTTTGTCC TAAACCCGTG TTTGCCTTCC ATCATGCTAA CTTCCCACAA 
TATGTAATGG ATGTGTTGAT GGATCAGCAC TTTACAGAAC CAACTCCAAT TCAGTGCCAG 
GGATTTCCGT TGGCTCTTAG TGGCCGGGAT ATGGTGGGCA TTGCTCAGAC TGGCTCTGGG 
AAGACGTTGG CGTATCTCCT GCCTGCAATT GTTCATATTA ACCACCAGCC ATACTTGGAA 
AGGGGAGATG GCCCAATCTG TCTAGTTCTG GCTCCTACCA GAGAGCTTGC CCAGCAAGTA 
CAGCAGGTGG CCGATGACTA TGGCAAATGT TCTAGATTGA AGAGTACTTG TATTTATGGA 
GGTGCTCCTA AAGGTCCCCA GATTCGAGAC TTGGAAAGAG GTGTTGAGAT CTGCATAGCC 
ACTCCTGGAC GTCTGATAGA TTTCCTGGAG TCAGGAAAGA CAAATCTTCG CCGATGTACT 
TACCTTGTAT TGGACGAAGC TGACAGAATG CTTGATATGG GGTTTGAACC CCAGATCCGT 
AAAATTGTTG ACCAAATCAG GCCTGATAGG CAGACACTGA TGTGGAGTGC AACCTGGCCA 
AAAGAAGTAA GACAGCTTGC AGAGGATTTC CTTCGTGATT ACACCCAGAT CAACGTAGGC 
AATCTGGAGT TGAGTGCCAA CCACAACATC CTCCAGATAG TGGATGTCTG CATGGAAAGT 
GAAAAAGACC ACAAGTTGAT CCAACTAATG GAAGAAATAA TGGCTGAAAA GGAAAACAAA 
ACAATAATAT TTGTGGAGAC AAAGAGACGC TGTGATGATC TGACTCGAAG GATGCGCAGA 
GATGGTTGGC CAGCTATGTG TATCCATGGA GACAAGAGTC AACCAGAAAG AGATTGGGTA 
CTTAATGAGT TCCGTTCTGG AAAGGCACCC ATCCTTATTG CTACAGATGT AGCCTCCCGT 
GGGCTAGATG TGGAAGATGT CAAGTTTGTG ATCAACTATG ACTATCCAAA CAGCTCAGAG 
GATTATGTGC ACCGTATTGG CCGAACAGCC CGTAGCACCA ACAAGGGTAC CGCCTATACC 
TTCTTCACCC CAGGGAACCT AAAACAGGCC AGAGAGCTTA TCAAAGTGCT GGAAGAGGCC 
AATCAGGCTA TCAATCCAAA ACTGATGCAG CTTGTGGACC ACAGAGGAGG CGGCGGAGGC 
GGGGGTGGTC GTTCTCGTTA CCGGACCACT TCTTCAGCCA ACAATCCCAA TCTGATGTAT 
CAGGATGAGT GTGACCGAAG GCTTCGAGGA GTCAAGGATG GTGGCCGGAG AGACTCTGCA 
AGCTATCGGG ATCGTAGTGA AACCGATAGA GCTGGTTATG CTAATGGCAG TGGCTATGGA 
AGTCCAAATT CTGCCTTTGG AGCACAAGCA GGCCAATACA CCTATGGTCA AGGCACCTAT 
GGGGCAGCTG CTTATGGCAC CAGTAGCTAT ACAGCTCAAG AATATGGTGC TGGCACTTAT 
GGAGCTAGTA GCACCACCTC AACTGGGAGA AGTTCACAGA GCTCTAGCCA GCAGTTTAGT 
GGGATAGGCC GGTCTGGGCA GCAGCCACAG CCACTGATGT CACAACAGTT TGCACAGCCT 
CCGGGAGCTA CCAATATGAT AGGTTACATG GGGCAGACTG CCTACCAATA CCCTCCTCCT 
CCTCCCCCTC CTCCTCCTTC ACGTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006377.2
CDS	75..2264
Translation	LPTGFVAPILCVLLPSPTREAATVASATGDSASERESAAPAAAPTAEAPPPSVVTRPEPQALPSPAIRAPLPDLYPFGTMRGGGFGDRDRDRDRGGFGARGGGGLPPKKFGNPGERLRKKKWDLSELPKFEKNFYVEHPEVARLTPYEVDELRRKKEITVRGGDVCPKPVFAFHHANFPQYVMDVLMDQHFTEPTPIQCQGFPLALSGRDMVGIAQTGSGKTLAYLLPAIVHINHQPYLERGDGPICLVLAPTRELAQQVQQVADDYGKCSRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLESGKTNLRRCTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLRDYTQINVGNLELSANHNILQIVDVCMESEKDHKLIQLMEEIMAEKENKTIIFVETKRRCDDLTRRMRRDGWPAMCIHGDKSQPERDWVLNEFRSGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYVHRIGRTARSTNKGTAYTFFTPGNLKQARELIKVLEEANQAINPKLMQLVDHRGGGGGGGGRSRYRTTSSANNPNLMYQDECDRRLRGVKDGGRRDSASYRDRSETDRAGYANGSGYGSPNSAFGAQAGQYTYGQGTYGAAAYGTSSYTAQEYGAGTYGASSTTSTGRSSQSSSQQFSGIGRSGQQPQPLMSQQFAQPPGATNMIGYMGQTAYQYPPPPPPPPPSRK

Target ORF information:

RefSeq Version	NM_006386.5
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 17 (DDX17), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006386.5

ORF Insert Sequence:

CTGCCCACCG GCTTTGTAGC CCCGATTCTC TGTGTTTTGC TCCCGTCTCC GACGAGAGAG 
GCGGCGACGG TGGCGTCTGC GACGGGAGAC AGCGCGTCGG AGCGAGAGAG CGCTGCGCCT 
GCCGCCGCCC CAACAGCGGA GGCGCCGCCG CCATCGGTCG TCACCAGACC GGAGCCGCAG 
GCCCTCCCGA GCCCGGCCAT CCGTGCCCCG CTCCCAGATC TCTATCCTTT TGGGACCATG 
CGCGGAGGAG GCTTTGGGGA CCGGGACCGG GATCGTGACC GTGGAGGATT TGGAGCAAGA 
GGTGGTGGTG GCCTTCCCCC GAAGAAATTT GGTAATCCTG GGGAGCGTTT GCGTAAAAAA 
AAGTGGGATT TGAGTGAGCT CCCCAAGTTT GAGAAAAATT TTTATGTGGA ACATCCGGAA 
GTAGCAAGGC TGACACCATA TGAGGTTGAT GAGCTACGCC GAAAGAAGGA GATTACAGTG 
AGGGGGGGAG ATGTTTGTCC TAAACCCGTG TTTGCCTTCC ATCATGCTAA CTTCCCACAA 
TATGTAATGG ATGTGTTGAT GGATCAGCAC TTTACAGAAC CAACTCCAAT TCAGTGCCAG 
GGATTTCCGT TGGCTCTTAG TGGCCGGGAT ATGGTGGGCA TTGCTCAGAC TGGCTCTGGG 
AAGACGTTGG CGTATCTCCT GCCTGCAATT GTTCATATTA ACCACCAGCC ATACTTGGAA 
AGGGGAGATG GCCCAATCTG TCTAGTTCTG GCTCCTACCA GAGAGCTTGC CCAGCAAGTA 
CAGCAGGTGG CCGATGACTA TGGCAAATGT TCTAGATTGA AGAGTACTTG TATTTATGGA 
GGTGCTCCTA AAGGTCCCCA GATTCGAGAC TTGGAAAGAG GTGTTGAGAT CTGCATAGCC 
ACTCCTGGAC GTCTGATAGA TTTCCTGGAG TCAGGAAAGA CAAATCTTCG CCGATGTACT 
TACCTTGTAT TGGACGAAGC TGACAGAATG CTTGATATGG GGTTTGAACC CCAGATCCGT 
AAAATTGTTG ACCAAATCAG GCCTGATAGG CAGACACTGA TGTGGAGTGC AACCTGGCCA 
AAAGAAGTAA GACAGCTTGC AGAGGATTTC CTTCGTGATT ACACCCAGAT CAACGTAGGC 
AATCTGGAGT TGAGTGCCAA CCACAACATC CTCCAGATAG TGGATGTCTG CATGGAAAGT 
GAAAAAGACC ACAAGTTGAT CCAACTAATG GAAGAAATAA TGGCTGAAAA GGAAAACAAA 
ACAATAATAT TTGTGGAGAC AAAGAGACGC TGTGATGATC TGACTCGAAG GATGCGCAGA 
GATGGTTGGC CAGCTATGTG TATCCATGGA GACAAGAGTC AACCAGAAAG AGATTGGGTA 
CTTAATGAGT TCCGTTCTGG AAAGGCACCC ATCCTTATTG CTACAGATGT AGCCTCCCGT 
GGGCTAGATG TGGAAGATGT CAAGTTTGTG ATCAACTATG ACTATCCAAA CAGCTCAGAG 
GATTATGTGC ACCGTATTGG CCGAACAGCC CGTAGCACCA ACAAGGGTAC CGCCTATACC 
TTCTTCACCC CAGGGAACCT AAAACAGGCC AGAGAGCTTA TCAAAGTGCT GGAAGAGGCC 
AATCAGGCTA TCAATCCAAA ACTGATGCAG CTTGTGGACC ACAGAGGAGG CGGCGGAGGC 
GGGGGTGGTC GTTCTCGTTA CCGGACCACT TCTTCAGCCA ACAATCCCAA TCTGATGTAT 
CAGGATGAGT GTGACCGAAG GCTTCGAGGA GTCAAGGATG GTGGCCGGAG AGACTCTGCA 
AGCTATCGGG ATCGTAGTGA AACCGATAGA GCTGGTTATG CTAATGGCAG TGGCTATGGA 
AGTCCAAATT CTGCCTTTGG AGCACAAGCA GGCCAATACA CCTATGGTCA AGGCACCTAT 
GGGGCAGCTG CTTATGGCAC CAGTAGCTAT ACAGCTCAAG AATATGGTGC TGGCACTTAT 
GGAGCTAGTA GCACCACCTC AACTGGGAGA AGTTCACAGA GCTCTAGCCA GCAGTTTAGT 
GGGATAGGCC GGTCTGGGCA GCAGCCACAG CCACTGATGT CACAACAGTT TGCACAGCCT 
CCGGGAGCTA CCAATATGAT AGGTTACATG GGGCAGACTG CCTACCAATA CCCTCCTCCT 
CCTCCCCCTC CTCCTCCTTC ACGTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Exome sequencing identifies targets in the treatment-resistant ophthalmoplegic subphenotype of myasthenia gravis.
Neuromuscular disorders : NMD27(9)816-825(2017 Sep)
Nel M,Jalali Sefid Dashti M,Gamieldien J,Heckmann JM

DDX17 nucleocytoplasmic shuttling promotes acquired gefitinib resistance in non-small cell lung cancer cells via activation of ?-catenin.
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Li K,Mo C,Gong D,Chen Y,Huang Z,Li Y,Zhang J,Huang L,Li Y,Fuller-Pace FV,Lin P,Wei Y

The miRNA biogenesis factors, p72/DDX17 and KHSRP regulate the protein level of Ago2 in human cells.
Biochimica et biophysica acta1859(10)1299-305(2016 10)
Connerty P,Bajan S,Remenyi J,Fuller-Pace FV,Hutvagner G

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DDX17 cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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