SAP18 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SAP18
Entrez Gene ID	10284
Full Name	Sin3A associated protein 18
Synonyms	2HOR0202,SAP18P
General protein information	Preferred Names Sin3A associated protein 18 Names histone deacetylase complex subunit SAP18 18 kDa Sin3-associated polypeptide Sin3A-associated protein, 18kDa cell growth inhibiting protein 38 cell growth-inhibiting gene 38 protein histone deacetlyase complex subunit SAP18 sin3-associated polypeptide, 18 kDa sin3-associated polypeptide, p18
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q12.11
Summary	Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2008].
Disorder MIM:	602949

mRNA and Protein(s)

mRNA	Protein	Name
XM_011534868.2	XP_011533170.1	histone deacetylase complex subunit SAP18 isoform X1
NM_005870.4	NP_005861.2	histone deacetylase complex subunit SAP18 isoform 1
NM_001366643.1	NP_001353572.1	histone deacetylase complex subunit SAP18 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gm10094	NP_001135913.1
Arabidopsis thaliana (thale cress)	SAP18	NP_566050.1
Rattus norvegicus (Norway rat)	Sap18	NP_001028857.2
Gallus gallus (chicken)	SAP18	NP_989643.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003036	XP_311841.2
Homo sapiens (human)	SAP18	NP_005861.2
Pan troglodytes (chimpanzee)	SAP18	XP_509567.3
Mus musculus (house mouse)	Sap18	NP_033145.2
Drosophila melanogaster (fruit fly)	Bin1	NP_524377.1
Oryza sativa (rice)	Os02g0122000	NP_001045723.1
Canis lupus familiaris (dog)	SAP18	NP_001239090.1
Rattus norvegicus (Norway rat)	RGD1561590	XP_002727857.1
Danio rerio (zebrafish)	sap18	NP_957014.1
Xenopus tropicalis (tropical clawed frog)	sap18	NP_001016166.1
	SAP18	XP_001085342.2
Bos taurus (cattle)	SAP18	NP_001030544.2
Caenorhabditis elegans (roundworm)	CELE_C16C10.4	NP_497833.1

Gene Ontology
Bibliography

Related articles in PubMed

Sin3A-associated protein, 18 kDa, a novel binding partner of TRIB1, regulates MTTP expression.
Makishima S, Boonvisut S, Ishizuka Y, Watanabe K, Nakayama K, Iwamoto S
Journal of lipid research56(6)1145-52(2015 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

The structure of the ASAP core complex reveals the existence of a Pinin-containing PSAP complex.
Murachelli AG, Ebert J, Basquin C, Le Hir H, Conti E
Nature structural & molecular biology19(4)378-86(2012 Mar)

Proteins associated with the exon junction complex also control the alternative splicing of apoptotic regulators.
Michelle L, Cloutier A, Toutant J, Shkreta L, Thibault P, Durand M, Garneau D, Gendron D, Lapointe E, Couture S, Le Hir H, Klinck R, Elela SA, Prinos P, Chabot B
Molecular and cellular biology32(5)954-67(2012 Mar)

Dynamic protein-protein interaction wiring of the human spliceosome.
Hegele A, Kamburov A, Grossmann A, Sourlis C, Wowro S, Weimann M, Will CL, Pena V, L?hrmann R, Stelzl U
Molecular cell45(4)567-80(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These studies indicated that SAP18 expression enhanced the recruitment of mSin3A in coordination with TRIB1 to MTTP regulatory elements and increased MTTP expression.
Title: Sin3A-associated protein, 18 kDa, a novel binding partner of TRIB1, regulates MTTP expression.

A novel function of SAP18 in splicing regulation.
Title: Human SAP18 mediates assembly of a splicing regulatory multiprotein complex via its ubiquitin-like fold.

study indicates HIV-1 integrase & INI1/hSNF5 bind SAP18 & recruit components of Sin3a-HDAC1 complex into HIV-1 virions; HIV-1 virion-associated HDAC1 is required for efficient early post-entry events, indicating novel role for HDAC1 in HIV-1 replication
Title: Recruitment of a SAP18-HDAC1 complex into HIV-1 virions and its requirement for viral replication.

The following SAP18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SAP18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11719	NM_005870.4 Latest version!	Homo sapiens Sin3A associated protein 18 (SAP18), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu63842	XM_011534868.2 Latest version!	Homo sapiens Sin3A associated protein 18kDa (SAP18), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu107037	NM_001366643.1 Latest version!	Homo sapiens Sin3A associated protein 18 (SAP18), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SAP18 ( NM_005870.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005861.2 Homo sapiens Sin3A associated protein 18 (SAP18), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu11719
Clone ID Related Accession (Same CDS sequence)	NM_005870.4
Accession Version	NM_005870.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	histone deacetylase complex subunit SAP18 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB171768.1, BC030836.1, AL158032.32 and BU618473.1. On Dec 3, 2008 this sequence version replaced NM_005870.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.155039.1, SRR1660807.125574.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTCGCTG CAGGGGTCGG AGGTCAGGGC GAGCGTCTCG CAGGCCGTAG GAGGAAGATG 
GCGGTGGAGT CGCGCGTTAC CCAGGAGGAA ATTAAGAAGG AGCCAGAGAA ACCGATCGAC 
CGCGAGAAGA CATGCCCACT GTTGCTACGG GTCTTCACCA CCAATAACGG CCGCCACCAC 
CGAATGGACG AGTTCTCCCG GGGAAATGTA CCGTCCAGCG AGTTGCAGAT CTACACTTGG 
ATGGATGCAA CCTTGAAAGA ACTGACAAGC TTAGTAAAAG AAGTCTACCC AGAAGCTAGA 
AAGAAGGGCA CTCACTTCAA TTTTGCAATC GTTTTTACAG ATGTTAAAAG ACCTGGCTAT 
CGAGTTAAGG AGATTGGCAG CACCATGTCT GGCAGAAAGG GGACTGATGA TTCCATGACC 
CTGCAGTCGC AGAAGTTCCA GATAGGAGAT TACTTGGACA TAGCAATTAC CCCTCCAAAT 
CGGGCACCAC CTCCTTCAGG GCGCATGAGA CCATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005861.2
CDS	40..558
Translation	MLAAGVGGQGERLAGRRRKMAVESRVTQEEIKKEPEKPIDREKTCPLLLRVFTTNNGRHHRMDEFSRGNVPSSELQIYTWMDATLKELTSLVKEVYPEARKKGTHFNFAIVFTDVKRPGYRVKEIGSTMSGRKGTDDSMTLQSQKFQIGDYLDIAITPPNRAPPPSGRMRPY

Target ORF information:

RefSeq Version	NM_005870.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Sin3A associated protein 18 (SAP18), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005870.4

ORF Insert Sequence:

ATGCTCGCTG CAGGGGTCGG AGGTCAGGGC GAGCGTCTCG CAGGCCGTAG GAGGAAGATG 
GCGGTGGAGT CGCGCGTTAC CCAGGAGGAA ATTAAGAAGG AGCCAGAGAA ACCGATCGAC 
CGCGAGAAGA CATGCCCACT GTTGCTACGG GTCTTCACCA CCAATAACGG CCGCCACCAC 
CGAATGGACG AGTTCTCCCG GGGAAATGTA CCGTCCAGCG AGTTGCAGAT CTACACTTGG 
ATGGATGCAA CCTTGAAAGA ACTGACAAGC TTAGTAAAAG AAGTCTACCC AGAAGCTAGA 
AAGAAGGGCA CTCACTTCAA TTTTGCAATC GTTTTTACAG ATGTTAAAAG ACCTGGCTAT 
CGAGTTAAGG AGATTGGCAG CACCATGTCT GGCAGAAAGG GGACTGATGA TTCCATGACC 
CTGCAGTCGC AGAAGTTCCA GATAGGAGAT TACTTGGACA TAGCAATTAC CCCTCCAAAT 
CGGGCACCAC CTCCTTCAGG GCGCATGAGA CCATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SAP18 ( XM_011534868.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533170.1 Homo sapiens Sin3A associated protein 18kDa (SAP18), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu63842
Clone ID Related Accession (Same CDS sequence)	XM_011534868.2
Accession Version	XM_011534868.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	histone deacetylase complex subunit SAP18 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534868.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTATA AAATTGTATT CCAGCTGTGT ATAAGGATGG ATGCAACCTT GAAAGAACTG 
ACAAGCTTAG TAAAAGAAGT CTACCCAGAA GCTAGAAAGA AGGGCACTCA CTTCAATTTT 
GCAATCGTTT TTACAGATGT TAAAAGACCT GGCTATCGAG TTAAGGAGAT TGGCAGCACC 
ATGTCTGGCA GAAAGGGGAC TGATGATTCC ATGACCCTGC AGTCGCAGAA GTTCCAGATA 
GGAGATTACT TGGACATAGC AATTACCCCT CCAAATCGGG CACCACCTCC TTCAGGGCGC 
ATGAGACCAT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533170.1
CDS	124..438
Translation	MMYKIVFQLCIRMDATLKELTSLVKEVYPEARKKGTHFNFAIVFTDVKRPGYRVKEIGSTMSGRKGTDDSMTLQSQKFQIGDYLDIAITPPNRAPPPSGRMRPY

Target ORF information:

RefSeq Version	XM_011534868.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Sin3A associated protein 18kDa (SAP18), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534868.2

ORF Insert Sequence:

ATGATGTATA AAATTGTATT CCAGCTGTGT ATAAGGATGG ATGCAACCTT GAAAGAACTG 
ACAAGCTTAG TAAAAGAAGT CTACCCAGAA GCTAGAAAGA AGGGCACTCA CTTCAATTTT 
GCAATCGTTT TTACAGATGT TAAAAGACCT GGCTATCGAG TTAAGGAGAT TGGCAGCACC 
ATGTCTGGCA GAAAGGGGAC TGATGATTCC ATGACCCTGC AGTCGCAGAA GTTCCAGATA 
GGAGATTACT TGGACATAGC AATTACCCCT CCAAATCGGG CACCACCTCC TTCAGGGCGC 
ATGAGACCAT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SAP18 ( NM_001366643.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353572.1 Homo sapiens Sin3A associated protein 18 (SAP18), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu107037
Clone ID Related Accession (Same CDS sequence)	NM_001366643.1
Accession Version	NM_001366643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	histone deacetylase complex subunit SAP18 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL158032.32. On Oct 14, 2018 this sequence version replaced XM_011534868.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.12891.1, SRR1660805.225831.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END##

ATGTACCGTC CAGCGAGTTG CAGATCTACA CTTGAGTCTT GCTCTGTCAT CCAGGCTGGA 
GTGCAGTGGC GCGATCTCGG CTCACTGCAA CCTCTGCCTC CCAGGTATAA GCGATTCTCC 
TGCCTCAGCC TCCTGAGTAG CTGGGATTAC AGGCGTGCAC CAAACTTCCT TTCATGCACA 
CAATTACTTA AAATGATGTA TAAAATTGTA TTCCAGCTGT GTATAAGGAT GGATGCAACC 
TTGAAAGAAC TGACAAGCTT AGTAAAAGAA GTCTACCCAG AAGCTAGAAA GAAGGGCACT 
CACTTCAATT TTGCAATCGT TTTTACAGAT GTTAAAAGAC CTGGCTATCG AGTTAAGGAG 
ATTGGCAGCA CCATGTCTGG CAGAAAGGGG ACTGATGATT CCATGACCCT GCAGTCGCAG 
AAGTTCCAGA TAGGAGATTA CTTGGACATA GCAATTACCC CTCCAAATCG GGCACCACCT 
CCTTCAGGGC GCATGAGACC ATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353572.1
CDS	213..719
Translation	MYRPASCRSTLESCSVIQAGVQWRDLGSLQPLPPRYKRFSCLSLLSSWDYRRAPNFLSCTQLLKMMYKIVFQLCIRMDATLKELTSLVKEVYPEARKKGTHFNFAIVFTDVKRPGYRVKEIGSTMSGRKGTDDSMTLQSQKFQIGDYLDIAITPPNRAPPPSGRMRPY

Target ORF information:

RefSeq Version	NM_001366643.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Sin3A associated protein 18 (SAP18), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366643.1

ORF Insert Sequence:

ATGTACCGTC CAGCGAGTTG CAGATCTACA CTTGAGTCTT GCTCTGTCAT CCAGGCTGGA 
GTGCAGTGGC GCGATCTCGG CTCACTGCAA CCTCTGCCTC CCAGGTATAA GCGATTCTCC 
TGCCTCAGCC TCCTGAGTAG CTGGGATTAC AGGCGTGCAC CAAACTTCCT TTCATGCACA 
CAATTACTTA AAATGATGTA TAAAATTGTA TTCCAGCTGT GTATAAGGAT GGATGCAACC 
TTGAAAGAAC TGACAAGCTT AGTAAAAGAA GTCTACCCAG AAGCTAGAAA GAAGGGCACT 
CACTTCAATT TTGCAATCGT TTTTACAGAT GTTAAAAGAC CTGGCTATCG AGTTAAGGAG 
ATTGGCAGCA CCATGTCTGG CAGAAAGGGG ACTGATGATT CCATGACCCT GCAGTCGCAG 
AAGTTCCAGA TAGGAGATTA CTTGGACATA GCAATTACCC CTCCAAATCG GGCACCACCT 
CCTTCAGGGC GCATGAGACC ATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sin3A-associated protein, 18 kDa, a novel binding partner of TRIB1, regulates MTTP expression.
Journal of lipid research56(6)1145-52(2015 Jun)
Makishima S,Boonvisut S,Ishizuka Y,Watanabe K,Nakayama K,Iwamoto S

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

The structure of the ASAP core complex reveals the existence of a Pinin-containing PSAP complex.
Nature structural & molecular biology19(4)378-86(2012 Mar)
Murachelli AG,Ebert J,Basquin C,Le Hir H,Conti E

Proteins associated with the exon junction complex also control the alternative splicing of apoptotic regulators.
Molecular and cellular biology32(5)954-67(2012 Mar)
Michelle L,Cloutier A,Toutant J,Shkreta L,Thibault P,Durand M,Garneau D,Gendron D,Lapointe E,Couture S,Le Hir H,Klinck R,Elela SA,Prinos P,Chabot B

Dynamic protein-protein interaction wiring of the human spliceosome.
Molecular cell45(4)567-80(2012 Feb)
Hegele A,Kamburov A,Grossmann A,Sourlis C,Wowro S,Weimann M,Will CL,Pena V,L?hrmann R,Stelzl U

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SAP18 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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