AKAP8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AKAP8
Entrez Gene ID	10270
Full Name	A-kinase anchoring protein 8
Synonyms	AKAP 95,AKAP-8,AKAP-95,AKAP95
General protein information	Preferred Names A-kinase anchoring protein 8 Names A-kinase anchor protein 8 A kinase (PRKA) anchor protein 8 A-kinase anchor protein, 95kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.12
Summary	This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011].
Disorder MIM:	604692

mRNA and Protein(s)

mRNA	Protein	Name
XM_024451313.1	XP_024307081.1	A-kinase anchor protein 8 isoform X3
XM_017026141.1	XP_016881630.1	A-kinase anchor protein 8 isoform X1
XM_011527624.2	XP_011525926.1	A-kinase anchor protein 8 isoform X2
NM_005858.3	NP_005849.1	A-kinase anchor protein 8
XM_017026141.2	XP_016881630.1	A-kinase anchor protein 8 isoform X1
XM_011527624.3	XP_011525926.1	A-kinase anchor protein 8 isoform X2
NM_005858.4	NP_005849.1	A-kinase anchor protein 8

Pathways from BioSystems

Homologies

Bos taurus (cattle)	AKAP8	NP_001095542.1
Pan troglodytes (chimpanzee)	AKAP8	XP_001159349.2
Canis lupus familiaris (dog)	AKAP8	NP_001007124.1
Mus musculus (house mouse)	Akap8	NP_062748.2
Homo sapiens (human)	AKAP8	NP_005849.1
	AKAP8	XP_001112993.2
Rattus norvegicus (Norway rat)	Akap8	NP_446307.1
Gallus gallus (chicken)	AKAP8	XP_004950089.1

Gene Ontology
Bibliography

Related articles in PubMed

PKA-binding domain of AKAP8 is essential for direct interaction with DPY30 protein.
Bieluszewska A, Weglewska M, Bieluszewski T, Lesniewicz K, Poreba E
The FEBS journal(2017 Dec)

AKAP95 interacts with nucleoporin TPR in mitosis and is important for the spindle assembly checkpoint.
L?pez-Soop G, R?nningen T, Rogala A, Richartz N, Blomhoff HK, Thiede B, Collas P, K?ntziger T
Cell cycle (Georgetown, Tex.)16(10)947-956(2017 May)

The Truncated Isoform of the Receptor Tyrosine Kinase ALK Generated by Alternative Transcription Initiation (ALK
Takakura Y, Yamaguchi N, Honda T, Morii M, Yuki R, Nakayama Y, Yamaguchi N
Biological & pharmaceutical bulletin40(11)1968-1975(2017)

AKAP95 regulates splicing through scaffolding RNAs and RNA processing factors.
Hu J, Khodadadi-Jamayran A, Mao M, Shah K, Yang Z, Nasim MT, Wang Z, Jiang H
Nature communications713347(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data indicate that AKAP95 is a novel nucleolus-associated protein with a regulatory role on rRNA production.
Title: A-kinase anchoring protein AKAP95 is a novel regulator of ribosomal RNA synthesis.

These results suggest that AKAP8 is involved in the regulation of chromatin structural changes through nuclear tyrosine phosphorylation.
Title: Role for Tyrosine Phosphorylation of A-kinase Anchoring Protein 8 (AKAP8) in Its Dissociation from Chromatin and the Nuclear Matrix.

Report expression of connexin 43 in ovarian cancer cells in G1/S phase.
Title: Roles of Cx43 and AKAP95 in ovarian cancer tissues in G1/S phase.

Gene dosage at 19p13.12, and AKAP8 and/or AKAP8L in particular, play an important role in modulation of head size and may contribute to autism risk.
Title: Reciprocal Relationship between Head Size, an Autism Endophenotype, and Gene Dosage at 19p13.12 Points to AKAP8 and AKAP8L.

Ectopic AKAP95 stimulates expression of a chromosomal reporter gene in synergy with MLL1 or MLL2, whereas AKAP95 depletion impairs retinoic acid-mediated gene induction in embryonic stem cells
Title: Regulation of transcription by the MLL2 complex and MLL complex-associated AKAP95.

The following AKAP8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AKAP8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu63829	XM_011527624.3 Latest version!	Homo sapiens A-kinase anchoring protein 8 (AKAP8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu104597	XM_024451313.1 Latest version!	Homo sapiens A-kinase anchoring protein 8 (AKAP8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu88115	XM_017026141.2 Latest version!	Homo sapiens A-kinase anchoring protein 8 (AKAP8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OHu16747	NM_005858.4 Latest version!	Homo sapiens A-kinase anchoring protein 8 (AKAP8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
View Old Accession Versions >>
OHu88115	XM_017026141.1	Homo sapiens A-kinase anchoring protein 8 (AKAP8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OHu63829	XM_011527624.2	Homo sapiens A-kinase anchoring protein 8 (AKAP8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu16747	NM_005858.3	Homo sapiens A-kinase anchoring protein 8 (AKAP8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AKAP8 ( XM_011527624.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525926.1 Homo sapiens A-kinase anchoring protein 8 (AKAP8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu63829
Clone ID Related Accession (Same CDS sequence)	XM_011527624.2 , XM_011527624.3
Accession Version	XM_011527624.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	A-kinase anchor protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527624.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCGAC AATTCCGACT CCCTCATTGC CAAGATCAAC CAGCGTTTGG ACATGATGTC 
CAAGGAAGGA GCTCCTTCCG CTTCCAGCCG TTCGAGTCCT ATGACTCCAG GCCCTGCCTG 
CCGGAGCACA ACCCCTACCG CCCCAGCTAC AGCTACGACT ATGAGTTCGA CCTGGGGTCC 
GACCGCAATG GCAGCTTTGG GGGGCAGTAC AGTGAATGCC GAGACCCAGC CCGGGAGCGG 
GGCTCCCTTG ATGGCTTCAT GCGGGGCCGG GGCCAGGGCC GCTTCCAGGA CCGGAGCAAC 
CCTGGCACCT TCATGCGCAG CGACCCCTTC GTGCCCCCCG CTGCGTCCTC TGAGCCCCTG 
TCCACGCCCT GGAACGAGCT GAACTACGTG GGTGGACGGG GCCTGGGAGG GCCCTCCCCC 
AGCCGGCCAC CTCCGTCCCT CTTCTCCCAG TCCATGGCTC CCGACTACGG CGTGATGGGC 
ATGCAGGGGG CGGGCGGCTA TGACAGCACC ATGCCCTACG GATGTGGCCG CTCGCAGCCT 
CGGATGCGGG ATCGGGATCG GCCCAAGAGG AGAGGGTTTG ACCGCTTCGG ACCAGATGGC 
ACGGGCAGGA AACGGAAGCA GTTCCAACTT TACGAGGAGC CAGACACCAA ACTGGCCCGG 
GTTGACAGTG AAGGAGATTT CTCCGAAAAT GATGACGCAG CTGGTGACTT CCGCTCAGGA 
GATGAAGAAT TCAAGGGTGA GGATGAACTC TGCGACTCTG GGAGGCAAAG AGGAGAGAAG 
GAGGACGAGG ACGAGGATGT GAAGAAGAGA AGGGAAAAGC AAAGGAGAAG AGACAGGACG 
CGGGACCGTG CAGCCGACAG AATTCAGTTT GCCTGTTCTG TATGCAAGTT CCGTAGCTTT 
GATGACGAAG AGATCCAGAA GCATCTGCAA AGCAAATTTC ACAAAGAGAC CCTGCGGTTC 
ATAAGCACCA AGCTGCCCGA CAAGACCGTG GAGTTCCTCC AGGAATACAT TGTAAACAGA 
AATAAGAAAA TTGAGAAGCG GCGTCAGGAA TTGATGGAGA AAGAAACCGC AAAACCAAAA 
CCAGATCCTT TCAAAGGGAT TGGCCAGGAG CACTTCTTCA AGAAGATCGA GGCTGCTCAC 
TGCCTGGCCT GCGACATGCT AATTCCTGCA CAGCCGCAGC TCCTCCAGCG GCACCTGCAC 
TCCGTGGACC ACAATCACAA CCGCAGGTTG GCTGCTGAAC AGTTCAAGAA AACCAGTCTC 
CATGTGGCTA AGAGTGTTTT GAACAACAGA CATATAGTGA AGATGCTGGA AAAATACCTC 
AAGGGTGAGG ACCCTTTCAC CAGTGAAACT GTTGATCCAG AAATGGAAGG AGATGACAAT 
TTAGGAGGTG AGGATAAGAA AGAGACACCT GAGGAGGTGG CCGCGGACGT CTTAGCAGAG 
GTGATTACAG CAGCAGTGAG GGCCGTAGAT GGGGAAGGAG CGCCCGCTCC AGAGAGCAGC 
GGGGAGCCGG CTGAGGACGA AGGCCCCACG GACACAGCGG AGGCCGGTAG TGATCCTCAA 
GCCGAACAGC TGCTGGAAGA GCAGGTGCCC TGTGGAACGG CACATGAGAA GGGCGTCCCC 
AAGGCCAGAA GTGAGGCTGC AGAGGCTGGA AATGGCGCCG AGACAATGGC AGCAGAGGCA 
GAAAGTGCCC AAACCAGAGT TGCTCCTGCC CCAGCTGCCG CGGATGCTGA AGTGGAACAA 
ACTGATGCAG AGTCTAAAGA CGCTGTTCCC ACAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525926.1
CDS	464..2242
Translation	MHRQFRLPHCQDQPAFGHDVQGRSSFRFQPFESYDSRPCLPEHNPYRPSYSYDYEFDLGSDRNGSFGGQYSECRDPARERGSLDGFMRGRGQGRFQDRSNPGTFMRSDPFVPPAASSEPLSTPWNELNYVGGRGLGGPSPSRPPPSLFSQSMAPDYGVMGMQGAGGYDSTMPYGCGRSQPRMRDRDRPKRRGFDRFGPDGTGRKRKQFQLYEEPDTKLARVDSEGDFSENDDAAGDFRSGDEEFKGEDELCDSGRQRGEKEDEDEDVKKRREKQRRRDRTRDRAADRIQFACSVCKFRSFDDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIVNRNKKIEKRRQELMEKETAKPKPDPFKGIGQEHFFKKIEAAHCLACDMLIPAQPQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNRHIVKMLEKYLKGEDPFTSETVDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAESAQTRVAPAPAAADAEVEQTDAESKDAVPTE

Target ORF information:

RefSeq Version	XM_011527624.2
Organism	Homo sapiens(human)
Definition	Homo sapiens A-kinase anchoring protein 8 (AKAP8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527624.2

ORF Insert Sequence:

ATGCACCGAC AATTCCGACT CCCTCATTGC CAAGATCAAC CAGCGTTTGG ACATGATGTC 
CAAGGAAGGA GCTCCTTCCG CTTCCAGCCG TTCGAGTCCT ATGACTCCAG GCCCTGCCTG 
CCGGAGCACA ACCCCTACCG CCCCAGCTAC AGCTACGACT ATGAGTTCGA CCTGGGGTCC 
GACCGCAATG GCAGCTTTGG GGGGCAGTAC AGTGAATGCC GAGACCCAGC CCGGGAGCGG 
GGCTCCCTTG ATGGCTTCAT GCGGGGCCGG GGCCAGGGCC GCTTCCAGGA CCGGAGCAAC 
CCTGGCACCT TCATGCGCAG CGACCCCTTC GTGCCCCCCG CTGCGTCCTC TGAGCCCCTG 
TCCACGCCCT GGAACGAGCT GAACTACGTG GGTGGACGGG GCCTGGGAGG GCCCTCCCCC 
AGCCGGCCAC CTCCGTCCCT CTTCTCCCAG TCCATGGCTC CCGACTACGG CGTGATGGGC 
ATGCAGGGGG CGGGCGGCTA TGACAGCACC ATGCCCTACG GATGTGGCCG CTCGCAGCCT 
CGGATGCGGG ATCGGGATCG GCCCAAGAGG AGAGGGTTTG ACCGCTTCGG ACCAGATGGC 
ACGGGCAGGA AACGGAAGCA GTTCCAACTT TACGAGGAGC CAGACACCAA ACTGGCCCGG 
GTTGACAGTG AAGGAGATTT CTCCGAAAAT GATGACGCAG CTGGTGACTT CCGCTCAGGA 
GATGAAGAAT TCAAGGGTGA GGATGAACTC TGCGACTCTG GGAGGCAAAG AGGAGAGAAG 
GAGGACGAGG ACGAGGATGT GAAGAAGAGA AGGGAAAAGC AAAGGAGAAG AGACAGGACG 
CGGGACCGTG CAGCCGACAG AATTCAGTTT GCCTGTTCTG TATGCAAGTT CCGTAGCTTT 
GATGACGAAG AGATCCAGAA GCATCTGCAA AGCAAATTTC ACAAAGAGAC CCTGCGGTTC 
ATAAGCACCA AGCTGCCCGA CAAGACCGTG GAGTTCCTCC AGGAATACAT TGTAAACAGA 
AATAAGAAAA TTGAGAAGCG GCGTCAGGAA TTGATGGAGA AAGAAACCGC AAAACCAAAA 
CCAGATCCTT TCAAAGGGAT TGGCCAGGAG CACTTCTTCA AGAAGATCGA GGCTGCTCAC 
TGCCTGGCCT GCGACATGCT AATTCCTGCA CAGCCGCAGC TCCTCCAGCG GCACCTGCAC 
TCCGTGGACC ACAATCACAA CCGCAGGTTG GCTGCTGAAC AGTTCAAGAA AACCAGTCTC 
CATGTGGCTA AGAGTGTTTT GAACAACAGA CATATAGTGA AGATGCTGGA AAAATACCTC 
AAGGGTGAGG ACCCTTTCAC CAGTGAAACT GTTGATCCAG AAATGGAAGG AGATGACAAT 
TTAGGAGGTG AGGATAAGAA AGAGACACCT GAGGAGGTGG CCGCGGACGT CTTAGCAGAG 
GTGATTACAG CAGCAGTGAG GGCCGTAGAT GGGGAAGGAG CGCCCGCTCC AGAGAGCAGC 
GGGGAGCCGG CTGAGGACGA AGGCCCCACG GACACAGCGG AGGCCGGTAG TGATCCTCAA 
GCCGAACAGC TGCTGGAAGA GCAGGTGCCC TGTGGAACGG CACATGAGAA GGGCGTCCCC 
AAGGCCAGAA GTGAGGCTGC AGAGGCTGGA AATGGCGCCG AGACAATGGC AGCAGAGGCA 
GAAAGTGCCC AAACCAGAGT TGCTCCTGCC CCAGCTGCCG CGGATGCTGA AGTGGAACAA 
ACTGATGCAG AGTCTAAAGA CGCTGTTCCC ACAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP8 ( XM_011527624.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525926.1 Homo sapiens A-kinase anchoring protein 8 (AKAP8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu63829
Clone ID Related Accession (Same CDS sequence)	XM_011527624.2 , XM_011527624.3
Accession Version	XM_011527624.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	A-kinase anchor protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011527624.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCGAC AATTCCGACT CCCTCATTGC CAAGATCAAC CAGCGTTTGG ACATGATGTC 
CAAGGAAGGA GCTCCTTCCG CTTCCAGCCG TTCGAGTCCT ATGACTCCAG GCCCTGCCTG 
CCGGAGCACA ACCCCTACCG CCCCAGCTAC AGCTACGACT ATGAGTTCGA CCTGGGGTCC 
GACCGCAATG GCAGCTTTGG GGGGCAGTAC AGTGAATGCC GAGACCCAGC CCGGGAGCGG 
GGCTCCCTTG ATGGCTTCAT GCGGGGCCGG GGCCAGGGCC GCTTCCAGGA CCGGAGCAAC 
CCTGGCACCT TCATGCGCAG CGACCCCTTC GTGCCCCCCG CTGCGTCCTC TGAGCCCCTG 
TCCACGCCCT GGAACGAGCT GAACTACGTG GGTGGACGGG GCCTGGGAGG GCCCTCCCCC 
AGCCGGCCAC CTCCGTCCCT CTTCTCCCAG TCCATGGCTC CCGACTACGG CGTGATGGGC 
ATGCAGGGGG CGGGCGGCTA TGACAGCACC ATGCCCTACG GATGTGGCCG CTCGCAGCCT 
CGGATGCGGG ATCGGGATCG GCCCAAGAGG AGAGGGTTTG ACCGCTTCGG ACCAGATGGC 
ACGGGCAGGA AACGGAAGCA GTTCCAACTT TACGAGGAGC CAGACACCAA ACTGGCCCGG 
GTTGACAGTG AAGGAGATTT CTCCGAAAAT GATGACGCAG CTGGTGACTT CCGCTCAGGA 
GATGAAGAAT TCAAGGGTGA GGATGAACTC TGCGACTCTG GGAGGCAAAG AGGAGAGAAG 
GAGGACGAGG ACGAGGATGT GAAGAAGAGA AGGGAAAAGC AAAGGAGAAG AGACAGGACG 
CGGGACCGTG CAGCCGACAG AATTCAGTTT GCCTGTTCTG TATGCAAGTT CCGTAGCTTT 
GATGACGAAG AGATCCAGAA GCATCTGCAA AGCAAATTTC ACAAAGAGAC CCTGCGGTTC 
ATAAGCACCA AGCTGCCCGA CAAGACCGTG GAGTTCCTCC AGGAATACAT TGTAAACAGA 
AATAAGAAAA TTGAGAAGCG GCGTCAGGAA TTGATGGAGA AAGAAACCGC AAAACCAAAA 
CCAGATCCTT TCAAAGGGAT TGGCCAGGAG CACTTCTTCA AGAAGATCGA GGCTGCTCAC 
TGCCTGGCCT GCGACATGCT AATTCCTGCA CAGCCGCAGC TCCTCCAGCG GCACCTGCAC 
TCCGTGGACC ACAATCACAA CCGCAGGTTG GCTGCTGAAC AGTTCAAGAA AACCAGTCTC 
CATGTGGCTA AGAGTGTTTT GAACAACAGA CATATAGTGA AGATGCTGGA AAAATACCTC 
AAGGGTGAGG ACCCTTTCAC CAGTGAAACT GTTGATCCAG AAATGGAAGG AGATGACAAT 
TTAGGAGGTG AGGATAAGAA AGAGACACCT GAGGAGGTGG CCGCGGACGT CTTAGCAGAG 
GTGATTACAG CAGCAGTGAG GGCCGTAGAT GGGGAAGGAG CGCCCGCTCC AGAGAGCAGC 
GGGGAGCCGG CTGAGGACGA AGGCCCCACG GACACAGCGG AGGCCGGTAG TGATCCTCAA 
GCCGAACAGC TGCTGGAAGA GCAGGTGCCC TGTGGAACGG CACATGAGAA GGGCGTCCCC 
AAGGCCAGAA GTGAGGCTGC AGAGGCTGGA AATGGCGCCG AGACAATGGC AGCAGAGGCA 
GAAAGTGCCC AAACCAGAGT TGCTCCTGCC CCAGCTGCCG CGGATGCTGA AGTGGAACAA 
ACTGATGCAG AGTCTAAAGA CGCTGTTCCC ACAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525926.1
CDS	641..2419
Translation	MHRQFRLPHCQDQPAFGHDVQGRSSFRFQPFESYDSRPCLPEHNPYRPSYSYDYEFDLGSDRNGSFGGQYSECRDPARERGSLDGFMRGRGQGRFQDRSNPGTFMRSDPFVPPAASSEPLSTPWNELNYVGGRGLGGPSPSRPPPSLFSQSMAPDYGVMGMQGAGGYDSTMPYGCGRSQPRMRDRDRPKRRGFDRFGPDGTGRKRKQFQLYEEPDTKLARVDSEGDFSENDDAAGDFRSGDEEFKGEDELCDSGRQRGEKEDEDEDVKKRREKQRRRDRTRDRAADRIQFACSVCKFRSFDDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIVNRNKKIEKRRQELMEKETAKPKPDPFKGIGQEHFFKKIEAAHCLACDMLIPAQPQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNRHIVKMLEKYLKGEDPFTSETVDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAESAQTRVAPAPAAADAEVEQTDAESKDAVPTE

Target ORF information:

RefSeq Version	XM_011527624.3
Organism	Homo sapiens(human)
Definition	Homo sapiens A-kinase anchoring protein 8 (AKAP8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527624.3

ORF Insert Sequence:

ATGCACCGAC AATTCCGACT CCCTCATTGC CAAGATCAAC CAGCGTTTGG ACATGATGTC 
CAAGGAAGGA GCTCCTTCCG CTTCCAGCCG TTCGAGTCCT ATGACTCCAG GCCCTGCCTG 
CCGGAGCACA ACCCCTACCG CCCCAGCTAC AGCTACGACT ATGAGTTCGA CCTGGGGTCC 
GACCGCAATG GCAGCTTTGG GGGGCAGTAC AGTGAATGCC GAGACCCAGC CCGGGAGCGG 
GGCTCCCTTG ATGGCTTCAT GCGGGGCCGG GGCCAGGGCC GCTTCCAGGA CCGGAGCAAC 
CCTGGCACCT TCATGCGCAG CGACCCCTTC GTGCCCCCCG CTGCGTCCTC TGAGCCCCTG 
TCCACGCCCT GGAACGAGCT GAACTACGTG GGTGGACGGG GCCTGGGAGG GCCCTCCCCC 
AGCCGGCCAC CTCCGTCCCT CTTCTCCCAG TCCATGGCTC CCGACTACGG CGTGATGGGC 
ATGCAGGGGG CGGGCGGCTA TGACAGCACC ATGCCCTACG GATGTGGCCG CTCGCAGCCT 
CGGATGCGGG ATCGGGATCG GCCCAAGAGG AGAGGGTTTG ACCGCTTCGG ACCAGATGGC 
ACGGGCAGGA AACGGAAGCA GTTCCAACTT TACGAGGAGC CAGACACCAA ACTGGCCCGG 
GTTGACAGTG AAGGAGATTT CTCCGAAAAT GATGACGCAG CTGGTGACTT CCGCTCAGGA 
GATGAAGAAT TCAAGGGTGA GGATGAACTC TGCGACTCTG GGAGGCAAAG AGGAGAGAAG 
GAGGACGAGG ACGAGGATGT GAAGAAGAGA AGGGAAAAGC AAAGGAGAAG AGACAGGACG 
CGGGACCGTG CAGCCGACAG AATTCAGTTT GCCTGTTCTG TATGCAAGTT CCGTAGCTTT 
GATGACGAAG AGATCCAGAA GCATCTGCAA AGCAAATTTC ACAAAGAGAC CCTGCGGTTC 
ATAAGCACCA AGCTGCCCGA CAAGACCGTG GAGTTCCTCC AGGAATACAT TGTAAACAGA 
AATAAGAAAA TTGAGAAGCG GCGTCAGGAA TTGATGGAGA AAGAAACCGC AAAACCAAAA 
CCAGATCCTT TCAAAGGGAT TGGCCAGGAG CACTTCTTCA AGAAGATCGA GGCTGCTCAC 
TGCCTGGCCT GCGACATGCT AATTCCTGCA CAGCCGCAGC TCCTCCAGCG GCACCTGCAC 
TCCGTGGACC ACAATCACAA CCGCAGGTTG GCTGCTGAAC AGTTCAAGAA AACCAGTCTC 
CATGTGGCTA AGAGTGTTTT GAACAACAGA CATATAGTGA AGATGCTGGA AAAATACCTC 
AAGGGTGAGG ACCCTTTCAC CAGTGAAACT GTTGATCCAG AAATGGAAGG AGATGACAAT 
TTAGGAGGTG AGGATAAGAA AGAGACACCT GAGGAGGTGG CCGCGGACGT CTTAGCAGAG 
GTGATTACAG CAGCAGTGAG GGCCGTAGAT GGGGAAGGAG CGCCCGCTCC AGAGAGCAGC 
GGGGAGCCGG CTGAGGACGA AGGCCCCACG GACACAGCGG AGGCCGGTAG TGATCCTCAA 
GCCGAACAGC TGCTGGAAGA GCAGGTGCCC TGTGGAACGG CACATGAGAA GGGCGTCCCC 
AAGGCCAGAA GTGAGGCTGC AGAGGCTGGA AATGGCGCCG AGACAATGGC AGCAGAGGCA 
GAAAGTGCCC AAACCAGAGT TGCTCCTGCC CCAGCTGCCG CGGATGCTGA AGTGGAACAA 
ACTGATGCAG AGTCTAAAGA CGCTGTTCCC ACAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP8 ( XM_024451313.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307081.1 Homo sapiens A-kinase anchoring protein 8 (AKAP8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu104597
Clone ID Related Accession (Same CDS sequence)	XM_024451313.1
Accession Version	XM_024451313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	A-kinase anchor protein 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTGG ACAAGCAGAA TGGGGAGGAC AGAGGGAGCA GGGGGCTGTG GTGCACATGG 
GGTGGGCAGG CTGTGTCCCT AGCCCATCCT GGCCGCCTCA GGCCATGTGG GGAGTTTGGT 
GACTACTCCG GGCTCTCACT CCAGGCCCGT GTCCACCAGG AGTCTCTGAC AGTGCCTTGG 
GGCATCCTGG ACCCTGCCGC GTGGTGCAGG CAGTGGCAGG GCACAGGGTG CGGTGGCCAA 
GCTGCAGCCC CCCGACCAGG CCCTGGAGCT CAGCTCCTTT GCAGGCCACT CAGAGCGTCT 
CGAAGGGACT TGTCTGTTAC AGGAGAGAAG GAGGACGAGG ACGAGGATGT GAAGAAGAGA 
AGGGAAAAGC AAAGGAGAAG AGACAGGACG CGGGACCGTG CAGCCGACAG AATTCAGTTT 
GCCTGTTCTG TATGCAAGTT CCGTAGCTTT GATGACGAAG AGATCCAGAA GCATCTGCAA 
AGCAAATTTC ACAAAGAGAC CCTGCGGTTC ATAAGCACCA AGCTGCCCGA CAAGACCGTG 
GAGTTCCTCC AGGAATACAT TGTAAACAGA AATAAGAAAA TTGAGAAGCG GCGTCAGGAA 
TTGATGGAGA AAGAAACCGC AAAACCAAAA CCAGATCCTT TCAAAGGGAT TGGCCAGGAG 
CACTTCTTCA AGAAGATCGA GGCTGCTCAC TGCCTGGCCT GCGACATGCT AATTCCTGCA 
CAGCCGCAGC TCCTCCAGCG GCACCTGCAC TCCGTGGACC ACAATCACAA CCGCAGGTTG 
GCTGCTGAAC AGTTCAAGAA AACCAGTCTC CATGTGGCTA AGAGTGTTTT GAACAACAGA 
CATATAGTGA AGATGCTGGA AAAATACCTC AAGGGTGAGG ACCCTTTCAC CAGTGAAACT 
GTTGATCCAG AAATGGAAGG AGATGACAAT TTAGGAGGTG AGGATAAGAA AGAGACACCT 
GAGGAGGTGG CCGCGGACGT CTTAGCAGAG GTGATTACAG CAGCAGTGAG GGCCGTAGAT 
GGGGAAGGAG CGCCCGCTCC AGAGAGCAGC GGGGAGCCGG CTGAGGACGA AGGCCCCACG 
GACACAGCGG AGGCCGGTAG TGATCCTCAA GCCGAACAGC TGCTGGAAGA GCAGGTGCCC 
TGTGGAACGG CACATGAGAA GGGCGTCCCC AAGGCCAGAA GTGAGGCTGC AGAGGCTGGA 
AATGGCGCCG AGACAATGGC AGCAGAGGCA GAAAGTGCCC AAACCAGAGT TGCTCCTGCC 
CCAGCTGCCG CGGATGCTGA AGTGGAACAA ACTGATGCAG AGTCTAAAGA CGCTGTTCCC 
ACAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307081.1
CDS	615..1943
Translation	MRLDKQNGEDRGSRGLWCTWGGQAVSLAHPGRLRPCGEFGDYSGLSLQARVHQESLTVPWGILDPAAWCRQWQGTGCGGQAAAPRPGPGAQLLCRPLRASRRDLSVTGEKEDEDEDVKKRREKQRRRDRTRDRAADRIQFACSVCKFRSFDDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIVNRNKKIEKRRQELMEKETAKPKPDPFKGIGQEHFFKKIEAAHCLACDMLIPAQPQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNRHIVKMLEKYLKGEDPFTSETVDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAESAQTRVAPAPAAADAEVEQTDAESKDAVPTE

Target ORF information:

RefSeq Version	XM_024451313.1
Organism	Homo sapiens(human)
Definition	Homo sapiens A-kinase anchoring protein 8 (AKAP8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451313.1

ORF Insert Sequence:

ATGAGATTGG ACAAGCAGAA TGGGGAGGAC AGAGGGAGCA GGGGGCTGTG GTGCACATGG 
GGTGGGCAGG CTGTGTCCCT AGCCCATCCT GGCCGCCTCA GGCCATGTGG GGAGTTTGGT 
GACTACTCCG GGCTCTCACT CCAGGCCCGT GTCCACCAGG AGTCTCTGAC AGTGCCTTGG 
GGCATCCTGG ACCCTGCCGC GTGGTGCAGG CAGTGGCAGG GCACAGGGTG CGGTGGCCAA 
GCTGCAGCCC CCCGACCAGG CCCTGGAGCT CAGCTCCTTT GCAGGCCACT CAGAGCGTCT 
CGAAGGGACT TGTCTGTTAC AGGAGAGAAG GAGGACGAGG ACGAGGATGT GAAGAAGAGA 
AGGGAAAAGC AAAGGAGAAG AGACAGGACG CGGGACCGTG CAGCCGACAG AATTCAGTTT 
GCCTGTTCTG TATGCAAGTT CCGTAGCTTT GATGACGAAG AGATCCAGAA GCATCTGCAA 
AGCAAATTTC ACAAAGAGAC CCTGCGGTTC ATAAGCACCA AGCTGCCCGA CAAGACCGTG 
GAGTTCCTCC AGGAATACAT TGTAAACAGA AATAAGAAAA TTGAGAAGCG GCGTCAGGAA 
TTGATGGAGA AAGAAACCGC AAAACCAAAA CCAGATCCTT TCAAAGGGAT TGGCCAGGAG 
CACTTCTTCA AGAAGATCGA GGCTGCTCAC TGCCTGGCCT GCGACATGCT AATTCCTGCA 
CAGCCGCAGC TCCTCCAGCG GCACCTGCAC TCCGTGGACC ACAATCACAA CCGCAGGTTG 
GCTGCTGAAC AGTTCAAGAA AACCAGTCTC CATGTGGCTA AGAGTGTTTT GAACAACAGA 
CATATAGTGA AGATGCTGGA AAAATACCTC AAGGGTGAGG ACCCTTTCAC CAGTGAAACT 
GTTGATCCAG AAATGGAAGG AGATGACAAT TTAGGAGGTG AGGATAAGAA AGAGACACCT 
GAGGAGGTGG CCGCGGACGT CTTAGCAGAG GTGATTACAG CAGCAGTGAG GGCCGTAGAT 
GGGGAAGGAG CGCCCGCTCC AGAGAGCAGC GGGGAGCCGG CTGAGGACGA AGGCCCCACG 
GACACAGCGG AGGCCGGTAG TGATCCTCAA GCCGAACAGC TGCTGGAAGA GCAGGTGCCC 
TGTGGAACGG CACATGAGAA GGGCGTCCCC AAGGCCAGAA GTGAGGCTGC AGAGGCTGGA 
AATGGCGCCG AGACAATGGC AGCAGAGGCA GAAAGTGCCC AAACCAGAGT TGCTCCTGCC 
CCAGCTGCCG CGGATGCTGA AGTGGAACAA ACTGATGCAG AGTCTAAAGA CGCTGTTCCC 
ACAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP8 ( XM_017026141.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881630.1 Homo sapiens A-kinase anchoring protein 8 (AKAP8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu88115
Clone ID Related Accession (Same CDS sequence)	XM_017026141.1 , XM_017026141.2
Accession Version	XM_017026141.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	A-kinase anchor protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACATGG CCTCTTACGG CCCAGAGCCA TGCACCGACA ATTCCGACTC CCTCATTGCC 
AAGATCAACC AGCGTTTGGA CATGATGTCC AAGGAAGGAG GCAGGGGCGG GAGCGGCGGC 
GGTGGGGAGG GCATACAGGA CCGGGAGAGC TCCTTCCGCT TCCAGCCGTT CGAGTCCTAT 
GACTCCAGGC CCTGCCTGCC GGAGCACAAC CCCTACCGCC CCAGCTACAG CTACGACTAT 
GAGTTCGACC TGGGGTCCGA CCGCAATGGC AGCTTTGGGG GGCAGTACAG TGAATGCCGA 
GACCCAGCCC GGGAGCGGGG CTCCCTTGAT GGCTTCATGC GGGGCCGGGG CCAGGGCCGC 
TTCCAGGACC GGAGCAACCC TGGCACCTTC ATGCGCAGCG ACCCCTTCGT GCCCCCCGCT 
GCGTCCTCTG AGCCCCTGTC CACGCCCTGG AACGAGCTGA ACTACGTGGG TGGACGGGGC 
CTGGGAGGGC CCTCCCCCAG CCGGCCACCT CCGTCCCTCT TCTCCCAGTC CATGGCTCCC 
GACTACGGCG TGATGGGCAT GCAGGGGGCG GGCGGCTATG ACAGCACCAT GCCCTACGGA 
TGTGGCCGCT CGCAGCCTCG GATGCGGGAT CGGGATCGGC CCAAGAGGAG AGGGTTTGAC 
CGCTTCGGAC CAGATGGCAC GGGCAGGAAA CGGAAGCAGT TCCAACTTTA CGAGGAGCCA 
GACACCAAAC TGGCCCGGGT TGACAGTGAA GGAGATTTCT CCGAAAATGA TGACGCAGCT 
GGTGACTTCC GCTCAGGAGA TGAAGAATTC AAGGGTGAGG ATGAACTCTG CGACTCTGGG 
AGGCAAAGAG GAGAGAAGGA GGACGAGGAC GAGGATGTGA AGAAGAGAAG GGAAAAGCAA 
AGGAGAAGAG ACAGGACGCG GGACCGTGCA GCCGACAGAA TTCAGTTTGC CTGTTCTGTA 
TGCAAGTTCC GTAGCTTTGA TGACGAAGAG ATCCAGAAGC ATCTGCAAAG CAAATTTCAC 
AAAGAGACCC TGCGGTTCAT AAGCACCAAG CTGCCCGACA AGACCGTGGA GTTCCTCCAG 
GAATACATTG TAAACAGAAA TAAGAAAATT GAGAAGCGGC GTCAGGAATT GATGGAGAAA 
GAAACCGCAA AACCAAAACC AGATCCTTTC AAAGGGATTG GCCAGGAGCA CTTCTTCAAG 
AAGATCGAGG CTGCTCACTG CCTGGCCTGC GACATGCTAA TTCCTGCACA GCCGCAGCTC 
CTCCAGCGGC ACCTGCACTC CGTGGACCAC AATCACAACC GCAGGTTGGC TGCTGAACAG 
TTCAAGAAAA CCAGTCTCCA TGTGGCTAAG AGTGTTTTGA ACAACAGACA TATAGTGAAG 
ATGCTGGAAA AATACCTCAA GGGTGAGGAC CCTTTCACCA GTGAAACTGT TGATCCAGAA 
ATGGAAGGAG ATGACAATTT AGGAGGTGAG GATAAGAAAG AGACACCTGA GGAGGTGGCC 
GCGGACGTCT TAGCAGAGGT GATTACAGCA GCAGTGAGGG CCGTAGATGG GGAAGGAGCG 
CCCGCTCCAG AGAGCAGCGG GGAGCCGGCT GAGGACGAAG GCCCCACGGA CACAGCGGAG 
GCCGGTAGTG ATCCTCAAGC CGAACAGCTG CTGGAAGAGC AGGTGCCCTG TGGAACGGCA 
CATGAGAAGG GCGTCCCCAA GGCCAGAAGT GAGGCTGCAG AGGCTGGAAA TGGCGCCGAG 
ACAATGGCAG CAGAGGCAGA AAGTGCCCAA ACCAGAGTTG CTCCTGCCCC AGCTGCCGCG 
GATGCTGAAG TGGAACAAAC TGATGCAGAG TCTAAAGACG CTGTTCCCAC AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881630.1
CDS	438..2294
Translation	MHMASYGPEPCTDNSDSLIAKINQRLDMMSKEGGRGGSGGGGEGIQDRESSFRFQPFESYDSRPCLPEHNPYRPSYSYDYEFDLGSDRNGSFGGQYSECRDPARERGSLDGFMRGRGQGRFQDRSNPGTFMRSDPFVPPAASSEPLSTPWNELNYVGGRGLGGPSPSRPPPSLFSQSMAPDYGVMGMQGAGGYDSTMPYGCGRSQPRMRDRDRPKRRGFDRFGPDGTGRKRKQFQLYEEPDTKLARVDSEGDFSENDDAAGDFRSGDEEFKGEDELCDSGRQRGEKEDEDEDVKKRREKQRRRDRTRDRAADRIQFACSVCKFRSFDDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIVNRNKKIEKRRQELMEKETAKPKPDPFKGIGQEHFFKKIEAAHCLACDMLIPAQPQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNRHIVKMLEKYLKGEDPFTSETVDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAESAQTRVAPAPAAADAEVEQTDAESKDAVPTE

Target ORF information:

RefSeq Version	XM_017026141.1
Organism	Homo sapiens(human)
Definition	Homo sapiens A-kinase anchoring protein 8 (AKAP8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026141.1

ORF Insert Sequence:

ATGCACATGG CCTCTTACGG CCCAGAGCCA TGCACCGACA ATTCCGACTC CCTCATTGCC 
AAGATCAACC AGCGTTTGGA CATGATGTCC AAGGAAGGAG GCAGGGGCGG GAGCGGCGGC 
GGTGGGGAGG GCATACAGGA CCGGGAGAGC TCCTTCCGCT TCCAGCCGTT CGAGTCCTAT 
GACTCCAGGC CCTGCCTGCC GGAGCACAAC CCCTACCGCC CCAGCTACAG CTACGACTAT 
GAGTTCGACC TGGGGTCCGA CCGCAATGGC AGCTTTGGGG GGCAGTACAG TGAATGCCGA 
GACCCAGCCC GGGAGCGGGG CTCCCTTGAT GGCTTCATGC GGGGCCGGGG CCAGGGCCGC 
TTCCAGGACC GGAGCAACCC TGGCACCTTC ATGCGCAGCG ACCCCTTCGT GCCCCCCGCT 
GCGTCCTCTG AGCCCCTGTC CACGCCCTGG AACGAGCTGA ACTACGTGGG TGGACGGGGC 
CTGGGAGGGC CCTCCCCCAG CCGGCCACCT CCGTCCCTCT TCTCCCAGTC CATGGCTCCC 
GACTACGGCG TGATGGGCAT GCAGGGGGCG GGCGGCTATG ACAGCACCAT GCCCTACGGA 
TGTGGCCGCT CGCAGCCTCG GATGCGGGAT CGGGATCGGC CCAAGAGGAG AGGGTTTGAC 
CGCTTCGGAC CAGATGGCAC GGGCAGGAAA CGGAAGCAGT TCCAACTTTA CGAGGAGCCA 
GACACCAAAC TGGCCCGGGT TGACAGTGAA GGAGATTTCT CCGAAAATGA TGACGCAGCT 
GGTGACTTCC GCTCAGGAGA TGAAGAATTC AAGGGTGAGG ATGAACTCTG CGACTCTGGG 
AGGCAAAGAG GAGAGAAGGA GGACGAGGAC GAGGATGTGA AGAAGAGAAG GGAAAAGCAA 
AGGAGAAGAG ACAGGACGCG GGACCGTGCA GCCGACAGAA TTCAGTTTGC CTGTTCTGTA 
TGCAAGTTCC GTAGCTTTGA TGACGAAGAG ATCCAGAAGC ATCTGCAAAG CAAATTTCAC 
AAAGAGACCC TGCGGTTCAT AAGCACCAAG CTGCCCGACA AGACCGTGGA GTTCCTCCAG 
GAATACATTG TAAACAGAAA TAAGAAAATT GAGAAGCGGC GTCAGGAATT GATGGAGAAA 
GAAACCGCAA AACCAAAACC AGATCCTTTC AAAGGGATTG GCCAGGAGCA CTTCTTCAAG 
AAGATCGAGG CTGCTCACTG CCTGGCCTGC GACATGCTAA TTCCTGCACA GCCGCAGCTC 
CTCCAGCGGC ACCTGCACTC CGTGGACCAC AATCACAACC GCAGGTTGGC TGCTGAACAG 
TTCAAGAAAA CCAGTCTCCA TGTGGCTAAG AGTGTTTTGA ACAACAGACA TATAGTGAAG 
ATGCTGGAAA AATACCTCAA GGGTGAGGAC CCTTTCACCA GTGAAACTGT TGATCCAGAA 
ATGGAAGGAG ATGACAATTT AGGAGGTGAG GATAAGAAAG AGACACCTGA GGAGGTGGCC 
GCGGACGTCT TAGCAGAGGT GATTACAGCA GCAGTGAGGG CCGTAGATGG GGAAGGAGCG 
CCCGCTCCAG AGAGCAGCGG GGAGCCGGCT GAGGACGAAG GCCCCACGGA CACAGCGGAG 
GCCGGTAGTG ATCCTCAAGC CGAACAGCTG CTGGAAGAGC AGGTGCCCTG TGGAACGGCA 
CATGAGAAGG GCGTCCCCAA GGCCAGAAGT GAGGCTGCAG AGGCTGGAAA TGGCGCCGAG 
ACAATGGCAG CAGAGGCAGA AAGTGCCCAA ACCAGAGTTG CTCCTGCCCC AGCTGCCGCG 
GATGCTGAAG TGGAACAAAC TGATGCAGAG TCTAAAGACG CTGTTCCCAC AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP8 ( XM_017026141.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881630.1 Homo sapiens A-kinase anchoring protein 8 (AKAP8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu88115
Clone ID Related Accession (Same CDS sequence)	XM_017026141.1 , XM_017026141.2
Accession Version	XM_017026141.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	A-kinase anchor protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017026141.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACATGG CCTCTTACGG CCCAGAGCCA TGCACCGACA ATTCCGACTC CCTCATTGCC 
AAGATCAACC AGCGTTTGGA CATGATGTCC AAGGAAGGAG GCAGGGGCGG GAGCGGCGGC 
GGTGGGGAGG GCATACAGGA CCGGGAGAGC TCCTTCCGCT TCCAGCCGTT CGAGTCCTAT 
GACTCCAGGC CCTGCCTGCC GGAGCACAAC CCCTACCGCC CCAGCTACAG CTACGACTAT 
GAGTTCGACC TGGGGTCCGA CCGCAATGGC AGCTTTGGGG GGCAGTACAG TGAATGCCGA 
GACCCAGCCC GGGAGCGGGG CTCCCTTGAT GGCTTCATGC GGGGCCGGGG CCAGGGCCGC 
TTCCAGGACC GGAGCAACCC TGGCACCTTC ATGCGCAGCG ACCCCTTCGT GCCCCCCGCT 
GCGTCCTCTG AGCCCCTGTC CACGCCCTGG AACGAGCTGA ACTACGTGGG TGGACGGGGC 
CTGGGAGGGC CCTCCCCCAG CCGGCCACCT CCGTCCCTCT TCTCCCAGTC CATGGCTCCC 
GACTACGGCG TGATGGGCAT GCAGGGGGCG GGCGGCTATG ACAGCACCAT GCCCTACGGA 
TGTGGCCGCT CGCAGCCTCG GATGCGGGAT CGGGATCGGC CCAAGAGGAG AGGGTTTGAC 
CGCTTCGGAC CAGATGGCAC GGGCAGGAAA CGGAAGCAGT TCCAACTTTA CGAGGAGCCA 
GACACCAAAC TGGCCCGGGT TGACAGTGAA GGAGATTTCT CCGAAAATGA TGACGCAGCT 
GGTGACTTCC GCTCAGGAGA TGAAGAATTC AAGGGTGAGG ATGAACTCTG CGACTCTGGG 
AGGCAAAGAG GAGAGAAGGA GGACGAGGAC GAGGATGTGA AGAAGAGAAG GGAAAAGCAA 
AGGAGAAGAG ACAGGACGCG GGACCGTGCA GCCGACAGAA TTCAGTTTGC CTGTTCTGTA 
TGCAAGTTCC GTAGCTTTGA TGACGAAGAG ATCCAGAAGC ATCTGCAAAG CAAATTTCAC 
AAAGAGACCC TGCGGTTCAT AAGCACCAAG CTGCCCGACA AGACCGTGGA GTTCCTCCAG 
GAATACATTG TAAACAGAAA TAAGAAAATT GAGAAGCGGC GTCAGGAATT GATGGAGAAA 
GAAACCGCAA AACCAAAACC AGATCCTTTC AAAGGGATTG GCCAGGAGCA CTTCTTCAAG 
AAGATCGAGG CTGCTCACTG CCTGGCCTGC GACATGCTAA TTCCTGCACA GCCGCAGCTC 
CTCCAGCGGC ACCTGCACTC CGTGGACCAC AATCACAACC GCAGGTTGGC TGCTGAACAG 
TTCAAGAAAA CCAGTCTCCA TGTGGCTAAG AGTGTTTTGA ACAACAGACA TATAGTGAAG 
ATGCTGGAAA AATACCTCAA GGGTGAGGAC CCTTTCACCA GTGAAACTGT TGATCCAGAA 
ATGGAAGGAG ATGACAATTT AGGAGGTGAG GATAAGAAAG AGACACCTGA GGAGGTGGCC 
GCGGACGTCT TAGCAGAGGT GATTACAGCA GCAGTGAGGG CCGTAGATGG GGAAGGAGCG 
CCCGCTCCAG AGAGCAGCGG GGAGCCGGCT GAGGACGAAG GCCCCACGGA CACAGCGGAG 
GCCGGTAGTG ATCCTCAAGC CGAACAGCTG CTGGAAGAGC AGGTGCCCTG TGGAACGGCA 
CATGAGAAGG GCGTCCCCAA GGCCAGAAGT GAGGCTGCAG AGGCTGGAAA TGGCGCCGAG 
ACAATGGCAG CAGAGGCAGA AAGTGCCCAA ACCAGAGTTG CTCCTGCCCC AGCTGCCGCG 
GATGCTGAAG TGGAACAAAC TGATGCAGAG TCTAAAGACG CTGTTCCCAC AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881630.1
CDS	614..2470
Translation	MHMASYGPEPCTDNSDSLIAKINQRLDMMSKEGGRGGSGGGGEGIQDRESSFRFQPFESYDSRPCLPEHNPYRPSYSYDYEFDLGSDRNGSFGGQYSECRDPARERGSLDGFMRGRGQGRFQDRSNPGTFMRSDPFVPPAASSEPLSTPWNELNYVGGRGLGGPSPSRPPPSLFSQSMAPDYGVMGMQGAGGYDSTMPYGCGRSQPRMRDRDRPKRRGFDRFGPDGTGRKRKQFQLYEEPDTKLARVDSEGDFSENDDAAGDFRSGDEEFKGEDELCDSGRQRGEKEDEDEDVKKRREKQRRRDRTRDRAADRIQFACSVCKFRSFDDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIVNRNKKIEKRRQELMEKETAKPKPDPFKGIGQEHFFKKIEAAHCLACDMLIPAQPQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNRHIVKMLEKYLKGEDPFTSETVDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAESAQTRVAPAPAAADAEVEQTDAESKDAVPTE

Target ORF information:

RefSeq Version	XM_017026141.2
Organism	Homo sapiens(human)
Definition	Homo sapiens A-kinase anchoring protein 8 (AKAP8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026141.2

ORF Insert Sequence:

ATGCACATGG CCTCTTACGG CCCAGAGCCA TGCACCGACA ATTCCGACTC CCTCATTGCC 
AAGATCAACC AGCGTTTGGA CATGATGTCC AAGGAAGGAG GCAGGGGCGG GAGCGGCGGC 
GGTGGGGAGG GCATACAGGA CCGGGAGAGC TCCTTCCGCT TCCAGCCGTT CGAGTCCTAT 
GACTCCAGGC CCTGCCTGCC GGAGCACAAC CCCTACCGCC CCAGCTACAG CTACGACTAT 
GAGTTCGACC TGGGGTCCGA CCGCAATGGC AGCTTTGGGG GGCAGTACAG TGAATGCCGA 
GACCCAGCCC GGGAGCGGGG CTCCCTTGAT GGCTTCATGC GGGGCCGGGG CCAGGGCCGC 
TTCCAGGACC GGAGCAACCC TGGCACCTTC ATGCGCAGCG ACCCCTTCGT GCCCCCCGCT 
GCGTCCTCTG AGCCCCTGTC CACGCCCTGG AACGAGCTGA ACTACGTGGG TGGACGGGGC 
CTGGGAGGGC CCTCCCCCAG CCGGCCACCT CCGTCCCTCT TCTCCCAGTC CATGGCTCCC 
GACTACGGCG TGATGGGCAT GCAGGGGGCG GGCGGCTATG ACAGCACCAT GCCCTACGGA 
TGTGGCCGCT CGCAGCCTCG GATGCGGGAT CGGGATCGGC CCAAGAGGAG AGGGTTTGAC 
CGCTTCGGAC CAGATGGCAC GGGCAGGAAA CGGAAGCAGT TCCAACTTTA CGAGGAGCCA 
GACACCAAAC TGGCCCGGGT TGACAGTGAA GGAGATTTCT CCGAAAATGA TGACGCAGCT 
GGTGACTTCC GCTCAGGAGA TGAAGAATTC AAGGGTGAGG ATGAACTCTG CGACTCTGGG 
AGGCAAAGAG GAGAGAAGGA GGACGAGGAC GAGGATGTGA AGAAGAGAAG GGAAAAGCAA 
AGGAGAAGAG ACAGGACGCG GGACCGTGCA GCCGACAGAA TTCAGTTTGC CTGTTCTGTA 
TGCAAGTTCC GTAGCTTTGA TGACGAAGAG ATCCAGAAGC ATCTGCAAAG CAAATTTCAC 
AAAGAGACCC TGCGGTTCAT AAGCACCAAG CTGCCCGACA AGACCGTGGA GTTCCTCCAG 
GAATACATTG TAAACAGAAA TAAGAAAATT GAGAAGCGGC GTCAGGAATT GATGGAGAAA 
GAAACCGCAA AACCAAAACC AGATCCTTTC AAAGGGATTG GCCAGGAGCA CTTCTTCAAG 
AAGATCGAGG CTGCTCACTG CCTGGCCTGC GACATGCTAA TTCCTGCACA GCCGCAGCTC 
CTCCAGCGGC ACCTGCACTC CGTGGACCAC AATCACAACC GCAGGTTGGC TGCTGAACAG 
TTCAAGAAAA CCAGTCTCCA TGTGGCTAAG AGTGTTTTGA ACAACAGACA TATAGTGAAG 
ATGCTGGAAA AATACCTCAA GGGTGAGGAC CCTTTCACCA GTGAAACTGT TGATCCAGAA 
ATGGAAGGAG ATGACAATTT AGGAGGTGAG GATAAGAAAG AGACACCTGA GGAGGTGGCC 
GCGGACGTCT TAGCAGAGGT GATTACAGCA GCAGTGAGGG CCGTAGATGG GGAAGGAGCG 
CCCGCTCCAG AGAGCAGCGG GGAGCCGGCT GAGGACGAAG GCCCCACGGA CACAGCGGAG 
GCCGGTAGTG ATCCTCAAGC CGAACAGCTG CTGGAAGAGC AGGTGCCCTG TGGAACGGCA 
CATGAGAAGG GCGTCCCCAA GGCCAGAAGT GAGGCTGCAG AGGCTGGAAA TGGCGCCGAG 
ACAATGGCAG CAGAGGCAGA AAGTGCCCAA ACCAGAGTTG CTCCTGCCCC AGCTGCCGCG 
GATGCTGAAG TGGAACAAAC TGATGCAGAG TCTAAAGACG CTGTTCCCAC AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP8 ( NM_005858.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005849.1 Homo sapiens A-kinase anchoring protein 8 (AKAP8), mRNA.

Price & Availability↑

CloneID	OHu16747
Clone ID Related Accession (Same CDS sequence)	NM_005858.3 , NM_005858.4
Accession Version	NM_005858.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	A-kinase anchor protein 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR005936.1, Y11997.1, CA429585.1 and AI581319.1. This sequence is a reference standard in the RefSeqGene project. On May 13, 2011 this sequence version replaced NM_005858.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.161666.1, SRR1660807.43556.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCAGG GCTACGGAGG CTACGGGGCG TGGAGTGCTG GACCTGCCAA CACCCAGGGT 
GCATATGGAA CTGGTGTGGC CAGCTGGCAA GGTTATGAAA ACTACAATTA CTATGGCGCC 
CAGAACACCA GTGTCACCAC AGGCGCAACC TACAGCTACG GCCCAGCCTC GTGGGAGGCC 
GCCAAGGCCA ATGATGGCGG CCTGGCGGCC GGGGCCCCTG CCATGCACAT GGCCTCTTAC 
GGCCCAGAGC CATGCACCGA CAATTCCGAC TCCCTCATTG CCAAGATCAA CCAGCGTTTG 
GACATGATGT CCAAGGAAGG AGGCAGGGGC GGGAGCGGCG GCGGTGGGGA GGGCATACAG 
GACCGGGAGA GCTCCTTCCG CTTCCAGCCG TTCGAGTCCT ATGACTCCAG GCCCTGCCTG 
CCGGAGCACA ACCCCTACCG CCCCAGCTAC AGCTACGACT ATGAGTTCGA CCTGGGGTCC 
GACCGCAATG GCAGCTTTGG GGGGCAGTAC AGTGAATGCC GAGACCCAGC CCGGGAGCGG 
GGCTCCCTTG ATGGCTTCAT GCGGGGCCGG GGCCAGGGCC GCTTCCAGGA CCGGAGCAAC 
CCTGGCACCT TCATGCGCAG CGACCCCTTC GTGCCCCCCG CTGCGTCCTC TGAGCCCCTG 
TCCACGCCCT GGAACGAGCT GAACTACGTG GGTGGACGGG GCCTGGGAGG GCCCTCCCCC 
AGCCGGCCAC CTCCGTCCCT CTTCTCCCAG TCCATGGCTC CCGACTACGG CGTGATGGGC 
ATGCAGGGGG CGGGCGGCTA TGACAGCACC ATGCCCTACG GATGTGGCCG CTCGCAGCCT 
CGGATGCGGG ATCGGGATCG GCCCAAGAGG AGAGGGTTTG ACCGCTTCGG ACCAGATGGC 
ACGGGCAGGA AACGGAAGCA GTTCCAACTT TACGAGGAGC CAGACACCAA ACTGGCCCGG 
GTTGACAGTG AAGGAGATTT CTCCGAAAAT GATGACGCAG CTGGTGACTT CCGCTCAGGA 
GATGAAGAAT TCAAGGGTGA GGATGAACTC TGCGACTCTG GGAGGCAAAG AGGAGAGAAG 
GAGGACGAGG ACGAGGATGT GAAGAAGAGA AGGGAAAAGC AAAGGAGAAG AGACAGGACG 
CGGGACCGTG CAGCCGACAG AATTCAGTTT GCCTGTTCTG TATGCAAGTT CCGTAGCTTT 
GATGACGAAG AGATCCAGAA GCATCTGCAA AGCAAATTTC ACAAAGAGAC CCTGCGGTTC 
ATAAGCACCA AGCTGCCCGA CAAGACCGTG GAGTTCCTCC AGGAATACAT TGTAAACAGA 
AATAAGAAAA TTGAGAAGCG GCGTCAGGAA TTGATGGAGA AAGAAACCGC AAAACCAAAA 
CCAGATCCTT TCAAAGGGAT TGGCCAGGAG CACTTCTTCA AGAAGATCGA GGCTGCTCAC 
TGCCTGGCCT GCGACATGCT AATTCCTGCA CAGCCGCAGC TCCTCCAGCG GCACCTGCAC 
TCCGTGGACC ACAATCACAA CCGCAGGTTG GCTGCTGAAC AGTTCAAGAA AACCAGTCTC 
CATGTGGCTA AGAGTGTTTT GAACAACAGA CATATAGTGA AGATGCTGGA AAAATACCTC 
AAGGGTGAGG ACCCTTTCAC CAGTGAAACT GTTGATCCAG AAATGGAAGG AGATGACAAT 
TTAGGAGGTG AGGATAAGAA AGAGACACCT GAGGAGGTGG CCGCGGACGT CTTAGCAGAG 
GTGATTACAG CAGCAGTGAG GGCCGTAGAT GGGGAAGGAG CGCCCGCTCC AGAGAGCAGC 
GGGGAGCCGG CTGAGGACGA AGGCCCCACG GACACAGCGG AGGCCGGTAG TGATCCTCAA 
GCCGAACAGC TGCTGGAAGA GCAGGTGCCC TGTGGAACGG CACATGAGAA GGGCGTCCCC 
AAGGCCAGAA GTGAGGCTGC AGAGGCTGGA AATGGCGCCG AGACAATGGC AGCAGAGGCA 
GAAAGTGCCC AAACCAGAGT TGCTCCTGCC CCAGCTGCCG CGGATGCTGA AGTGGAACAA 
ACTGATGCAG AGTCTAAAGA CGCTGTTCCC ACAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005849.1
CDS	71..2149
Translation	MDQGYGGYGAWSAGPANTQGAYGTGVASWQGYENYNYYGAQNTSVTTGATYSYGPASWEAAKANDGGLAAGAPAMHMASYGPEPCTDNSDSLIAKINQRLDMMSKEGGRGGSGGGGEGIQDRESSFRFQPFESYDSRPCLPEHNPYRPSYSYDYEFDLGSDRNGSFGGQYSECRDPARERGSLDGFMRGRGQGRFQDRSNPGTFMRSDPFVPPAASSEPLSTPWNELNYVGGRGLGGPSPSRPPPSLFSQSMAPDYGVMGMQGAGGYDSTMPYGCGRSQPRMRDRDRPKRRGFDRFGPDGTGRKRKQFQLYEEPDTKLARVDSEGDFSENDDAAGDFRSGDEEFKGEDELCDSGRQRGEKEDEDEDVKKRREKQRRRDRTRDRAADRIQFACSVCKFRSFDDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIVNRNKKIEKRRQELMEKETAKPKPDPFKGIGQEHFFKKIEAAHCLACDMLIPAQPQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNRHIVKMLEKYLKGEDPFTSETVDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAESAQTRVAPAPAAADAEVEQTDAESKDAVPTE

Target ORF information:

RefSeq Version	NM_005858.3
Organism	Homo sapiens(human)
Definition	Homo sapiens A-kinase anchoring protein 8 (AKAP8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005858.3

ORF Insert Sequence:

ATGGACCAGG GCTACGGAGG CTACGGGGCG TGGAGTGCTG GACCTGCCAA CACCCAGGGT 
GCATATGGAA CTGGTGTGGC CAGCTGGCAA GGTTATGAAA ACTACAATTA CTATGGCGCC 
CAGAACACCA GTGTCACCAC AGGCGCAACC TACAGCTACG GCCCAGCCTC GTGGGAGGCC 
GCCAAGGCCA ATGATGGCGG CCTGGCGGCC GGGGCCCCTG CCATGCACAT GGCCTCTTAC 
GGCCCAGAGC CATGCACCGA CAATTCCGAC TCCCTCATTG CCAAGATCAA CCAGCGTTTG 
GACATGATGT CCAAGGAAGG AGGCAGGGGC GGGAGCGGCG GCGGTGGGGA GGGCATACAG 
GACCGGGAGA GCTCCTTCCG CTTCCAGCCG TTCGAGTCCT ATGACTCCAG GCCCTGCCTG 
CCGGAGCACA ACCCCTACCG CCCCAGCTAC AGCTACGACT ATGAGTTCGA CCTGGGGTCC 
GACCGCAATG GCAGCTTTGG GGGGCAGTAC AGTGAATGCC GAGACCCAGC CCGGGAGCGG 
GGCTCCCTTG ATGGCTTCAT GCGGGGCCGG GGCCAGGGCC GCTTCCAGGA CCGGAGCAAC 
CCTGGCACCT TCATGCGCAG CGACCCCTTC GTGCCCCCCG CTGCGTCCTC TGAGCCCCTG 
TCCACGCCCT GGAACGAGCT GAACTACGTG GGTGGACGGG GCCTGGGAGG GCCCTCCCCC 
AGCCGGCCAC CTCCGTCCCT CTTCTCCCAG TCCATGGCTC CCGACTACGG CGTGATGGGC 
ATGCAGGGGG CGGGCGGCTA TGACAGCACC ATGCCCTACG GATGTGGCCG CTCGCAGCCT 
CGGATGCGGG ATCGGGATCG GCCCAAGAGG AGAGGGTTTG ACCGCTTCGG ACCAGATGGC 
ACGGGCAGGA AACGGAAGCA GTTCCAACTT TACGAGGAGC CAGACACCAA ACTGGCCCGG 
GTTGACAGTG AAGGAGATTT CTCCGAAAAT GATGACGCAG CTGGTGACTT CCGCTCAGGA 
GATGAAGAAT TCAAGGGTGA GGATGAACTC TGCGACTCTG GGAGGCAAAG AGGAGAGAAG 
GAGGACGAGG ACGAGGATGT GAAGAAGAGA AGGGAAAAGC AAAGGAGAAG AGACAGGACG 
CGGGACCGTG CAGCCGACAG AATTCAGTTT GCCTGTTCTG TATGCAAGTT CCGTAGCTTT 
GATGACGAAG AGATCCAGAA GCATCTGCAA AGCAAATTTC ACAAAGAGAC CCTGCGGTTC 
ATAAGCACCA AGCTGCCCGA CAAGACCGTG GAGTTCCTCC AGGAATACAT TGTAAACAGA 
AATAAGAAAA TTGAGAAGCG GCGTCAGGAA TTGATGGAGA AAGAAACCGC AAAACCAAAA 
CCAGATCCTT TCAAAGGGAT TGGCCAGGAG CACTTCTTCA AGAAGATCGA GGCTGCTCAC 
TGCCTGGCCT GCGACATGCT AATTCCTGCA CAGCCGCAGC TCCTCCAGCG GCACCTGCAC 
TCCGTGGACC ACAATCACAA CCGCAGGTTG GCTGCTGAAC AGTTCAAGAA AACCAGTCTC 
CATGTGGCTA AGAGTGTTTT GAACAACAGA CATATAGTGA AGATGCTGGA AAAATACCTC 
AAGGGTGAGG ACCCTTTCAC CAGTGAAACT GTTGATCCAG AAATGGAAGG AGATGACAAT 
TTAGGAGGTG AGGATAAGAA AGAGACACCT GAGGAGGTGG CCGCGGACGT CTTAGCAGAG 
GTGATTACAG CAGCAGTGAG GGCCGTAGAT GGGGAAGGAG CGCCCGCTCC AGAGAGCAGC 
GGGGAGCCGG CTGAGGACGA AGGCCCCACG GACACAGCGG AGGCCGGTAG TGATCCTCAA 
GCCGAACAGC TGCTGGAAGA GCAGGTGCCC TGTGGAACGG CACATGAGAA GGGCGTCCCC 
AAGGCCAGAA GTGAGGCTGC AGAGGCTGGA AATGGCGCCG AGACAATGGC AGCAGAGGCA 
GAAAGTGCCC AAACCAGAGT TGCTCCTGCC CCAGCTGCCG CGGATGCTGA AGTGGAACAA 
ACTGATGCAG AGTCTAAAGA CGCTGTTCCC ACAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP8 ( NM_005858.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005849.1 Homo sapiens A-kinase anchoring protein 8 (AKAP8), mRNA.

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CloneID	OHu16747
Clone ID Related Accession (Same CDS sequence)	NM_005858.3 , NM_005858.4
Accession Version	NM_005858.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Homo sapiens(human)
Product	A-kinase anchor protein 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Y11997.1, CA429585.1, AI581319.1 and AC093060.2. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_005858.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.161666.1, SRR1803614.116134.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000269701.7/ ENSP00000269701.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACCAGG GCTACGGAGG CTACGGGGCG TGGAGTGCTG GACCTGCCAA CACCCAGGGT 
GCATATGGAA CTGGTGTGGC CAGCTGGCAA GGTTATGAAA ACTACAATTA CTATGGCGCC 
CAGAACACCA GTGTCACCAC AGGCGCAACC TACAGCTACG GCCCAGCCTC GTGGGAGGCC 
GCCAAGGCCA ATGATGGCGG CCTGGCGGCC GGGGCCCCTG CCATGCACAT GGCCTCTTAC 
GGCCCAGAGC CATGCACCGA CAATTCCGAC TCCCTCATTG CCAAGATCAA CCAGCGTTTG 
GACATGATGT CCAAGGAAGG AGGCAGGGGC GGGAGCGGCG GCGGTGGGGA GGGCATACAG 
GACCGGGAGA GCTCCTTCCG CTTCCAGCCG TTCGAGTCCT ATGACTCCAG GCCCTGCCTG 
CCGGAGCACA ACCCCTACCG CCCCAGCTAC AGCTACGACT ATGAGTTCGA CCTGGGGTCC 
GACCGCAATG GCAGCTTTGG GGGGCAGTAC AGTGAATGCC GAGACCCAGC CCGGGAGCGG 
GGCTCCCTTG ATGGCTTCAT GCGGGGCCGG GGCCAGGGCC GCTTCCAGGA CCGGAGCAAC 
CCTGGCACCT TCATGCGCAG CGACCCCTTC GTGCCCCCCG CTGCGTCCTC TGAGCCCCTG 
TCCACGCCCT GGAACGAGCT GAACTACGTG GGTGGACGGG GCCTGGGAGG GCCCTCCCCC 
AGCCGGCCAC CTCCGTCCCT CTTCTCCCAG TCCATGGCTC CCGACTACGG CGTGATGGGC 
ATGCAGGGGG CGGGCGGCTA TGACAGCACC ATGCCCTACG GATGTGGCCG CTCGCAGCCT 
CGGATGCGGG ATCGGGATCG GCCCAAGAGG AGAGGGTTTG ACCGCTTCGG ACCAGATGGC 
ACGGGCAGGA AACGGAAGCA GTTCCAACTT TACGAGGAGC CAGACACCAA ACTGGCCCGG 
GTTGACAGTG AAGGAGATTT CTCCGAAAAT GATGACGCAG CTGGTGACTT CCGCTCAGGA 
GATGAAGAAT TCAAGGGTGA GGATGAACTC TGCGACTCTG GGAGGCAAAG AGGAGAGAAG 
GAGGACGAGG ACGAGGATGT GAAGAAGAGA AGGGAAAAGC AAAGGAGAAG AGACAGGACG 
CGGGACCGTG CAGCCGACAG AATTCAGTTT GCCTGTTCTG TATGCAAGTT CCGTAGCTTT 
GATGACGAAG AGATCCAGAA GCATCTGCAA AGCAAATTTC ACAAAGAGAC CCTGCGGTTC 
ATAAGCACCA AGCTGCCCGA CAAGACCGTG GAGTTCCTCC AGGAATACAT TGTAAACAGA 
AATAAGAAAA TTGAGAAGCG GCGTCAGGAA TTGATGGAGA AAGAAACCGC AAAACCAAAA 
CCAGATCCTT TCAAAGGGAT TGGCCAGGAG CACTTCTTCA AGAAGATCGA GGCTGCTCAC 
TGCCTGGCCT GCGACATGCT AATTCCTGCA CAGCCGCAGC TCCTCCAGCG GCACCTGCAC 
TCCGTGGACC ACAATCACAA CCGCAGGTTG GCTGCTGAAC AGTTCAAGAA AACCAGTCTC 
CATGTGGCTA AGAGTGTTTT GAACAACAGA CATATAGTGA AGATGCTGGA AAAATACCTC 
AAGGGTGAGG ACCCTTTCAC CAGTGAAACT GTTGATCCAG AAATGGAAGG AGATGACAAT 
TTAGGAGGTG AGGATAAGAA AGAGACACCT GAGGAGGTGG CCGCGGACGT CTTAGCAGAG 
GTGATTACAG CAGCAGTGAG GGCCGTAGAT GGGGAAGGAG CGCCCGCTCC AGAGAGCAGC 
GGGGAGCCGG CTGAGGACGA AGGCCCCACG GACACAGCGG AGGCCGGTAG TGATCCTCAA 
GCCGAACAGC TGCTGGAAGA GCAGGTGCCC TGTGGAACGG CACATGAGAA GGGCGTCCCC 
AAGGCCAGAA GTGAGGCTGC AGAGGCTGGA AATGGCGCCG AGACAATGGC AGCAGAGGCA 
GAAAGTGCCC AAACCAGAGT TGCTCCTGCC CCAGCTGCCG CGGATGCTGA AGTGGAACAA 
ACTGATGCAG AGTCTAAAGA CGCTGTTCCC ACAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005849.1
CDS	57..2135
Translation	MDQGYGGYGAWSAGPANTQGAYGTGVASWQGYENYNYYGAQNTSVTTGATYSYGPASWEAAKANDGGLAAGAPAMHMASYGPEPCTDNSDSLIAKINQRLDMMSKEGGRGGSGGGGEGIQDRESSFRFQPFESYDSRPCLPEHNPYRPSYSYDYEFDLGSDRNGSFGGQYSECRDPARERGSLDGFMRGRGQGRFQDRSNPGTFMRSDPFVPPAASSEPLSTPWNELNYVGGRGLGGPSPSRPPPSLFSQSMAPDYGVMGMQGAGGYDSTMPYGCGRSQPRMRDRDRPKRRGFDRFGPDGTGRKRKQFQLYEEPDTKLARVDSEGDFSENDDAAGDFRSGDEEFKGEDELCDSGRQRGEKEDEDEDVKKRREKQRRRDRTRDRAADRIQFACSVCKFRSFDDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIVNRNKKIEKRRQELMEKETAKPKPDPFKGIGQEHFFKKIEAAHCLACDMLIPAQPQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNRHIVKMLEKYLKGEDPFTSETVDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAESAQTRVAPAPAAADAEVEQTDAESKDAVPTE

Target ORF information:

RefSeq Version	NM_005858.4
Organism	Homo sapiens(human)
Definition	Homo sapiens A-kinase anchoring protein 8 (AKAP8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005858.4

ORF Insert Sequence:

ATGGACCAGG GCTACGGAGG CTACGGGGCG TGGAGTGCTG GACCTGCCAA CACCCAGGGT 
GCATATGGAA CTGGTGTGGC CAGCTGGCAA GGTTATGAAA ACTACAATTA CTATGGCGCC 
CAGAACACCA GTGTCACCAC AGGCGCAACC TACAGCTACG GCCCAGCCTC GTGGGAGGCC 
GCCAAGGCCA ATGATGGCGG CCTGGCGGCC GGGGCCCCTG CCATGCACAT GGCCTCTTAC 
GGCCCAGAGC CATGCACCGA CAATTCCGAC TCCCTCATTG CCAAGATCAA CCAGCGTTTG 
GACATGATGT CCAAGGAAGG AGGCAGGGGC GGGAGCGGCG GCGGTGGGGA GGGCATACAG 
GACCGGGAGA GCTCCTTCCG CTTCCAGCCG TTCGAGTCCT ATGACTCCAG GCCCTGCCTG 
CCGGAGCACA ACCCCTACCG CCCCAGCTAC AGCTACGACT ATGAGTTCGA CCTGGGGTCC 
GACCGCAATG GCAGCTTTGG GGGGCAGTAC AGTGAATGCC GAGACCCAGC CCGGGAGCGG 
GGCTCCCTTG ATGGCTTCAT GCGGGGCCGG GGCCAGGGCC GCTTCCAGGA CCGGAGCAAC 
CCTGGCACCT TCATGCGCAG CGACCCCTTC GTGCCCCCCG CTGCGTCCTC TGAGCCCCTG 
TCCACGCCCT GGAACGAGCT GAACTACGTG GGTGGACGGG GCCTGGGAGG GCCCTCCCCC 
AGCCGGCCAC CTCCGTCCCT CTTCTCCCAG TCCATGGCTC CCGACTACGG CGTGATGGGC 
ATGCAGGGGG CGGGCGGCTA TGACAGCACC ATGCCCTACG GATGTGGCCG CTCGCAGCCT 
CGGATGCGGG ATCGGGATCG GCCCAAGAGG AGAGGGTTTG ACCGCTTCGG ACCAGATGGC 
ACGGGCAGGA AACGGAAGCA GTTCCAACTT TACGAGGAGC CAGACACCAA ACTGGCCCGG 
GTTGACAGTG AAGGAGATTT CTCCGAAAAT GATGACGCAG CTGGTGACTT CCGCTCAGGA 
GATGAAGAAT TCAAGGGTGA GGATGAACTC TGCGACTCTG GGAGGCAAAG AGGAGAGAAG 
GAGGACGAGG ACGAGGATGT GAAGAAGAGA AGGGAAAAGC AAAGGAGAAG AGACAGGACG 
CGGGACCGTG CAGCCGACAG AATTCAGTTT GCCTGTTCTG TATGCAAGTT CCGTAGCTTT 
GATGACGAAG AGATCCAGAA GCATCTGCAA AGCAAATTTC ACAAAGAGAC CCTGCGGTTC 
ATAAGCACCA AGCTGCCCGA CAAGACCGTG GAGTTCCTCC AGGAATACAT TGTAAACAGA 
AATAAGAAAA TTGAGAAGCG GCGTCAGGAA TTGATGGAGA AAGAAACCGC AAAACCAAAA 
CCAGATCCTT TCAAAGGGAT TGGCCAGGAG CACTTCTTCA AGAAGATCGA GGCTGCTCAC 
TGCCTGGCCT GCGACATGCT AATTCCTGCA CAGCCGCAGC TCCTCCAGCG GCACCTGCAC 
TCCGTGGACC ACAATCACAA CCGCAGGTTG GCTGCTGAAC AGTTCAAGAA AACCAGTCTC 
CATGTGGCTA AGAGTGTTTT GAACAACAGA CATATAGTGA AGATGCTGGA AAAATACCTC 
AAGGGTGAGG ACCCTTTCAC CAGTGAAACT GTTGATCCAG AAATGGAAGG AGATGACAAT 
TTAGGAGGTG AGGATAAGAA AGAGACACCT GAGGAGGTGG CCGCGGACGT CTTAGCAGAG 
GTGATTACAG CAGCAGTGAG GGCCGTAGAT GGGGAAGGAG CGCCCGCTCC AGAGAGCAGC 
GGGGAGCCGG CTGAGGACGA AGGCCCCACG GACACAGCGG AGGCCGGTAG TGATCCTCAA 
GCCGAACAGC TGCTGGAAGA GCAGGTGCCC TGTGGAACGG CACATGAGAA GGGCGTCCCC 
AAGGCCAGAA GTGAGGCTGC AGAGGCTGGA AATGGCGCCG AGACAATGGC AGCAGAGGCA 
GAAAGTGCCC AAACCAGAGT TGCTCCTGCC CCAGCTGCCG CGGATGCTGA AGTGGAACAA 
ACTGATGCAG AGTCTAAAGA CGCTGTTCCC ACAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PKA-binding domain of AKAP8 is essential for direct interaction with DPY30 protein.
The FEBS journal(2017 Dec)
Bieluszewska A,Weglewska M,Bieluszewski T,Lesniewicz K,Poreba E

AKAP95 interacts with nucleoporin TPR in mitosis and is important for the spindle assembly checkpoint.
Cell cycle (Georgetown, Tex.)16(10)947-956(2017 May)
L?pez-Soop G,R?nningen T,Rogala A,Richartz N,Blomhoff HK,Thiede B,Collas P,K?ntziger T

The Truncated Isoform of the Receptor Tyrosine Kinase ALK Generated by Alternative Transcription Initiation (ALK
Biological & pharmaceutical bulletin40(11)1968-1975(2017)
Takakura Y,Yamaguchi N,Honda T,Morii M,Yuki R,Nakayama Y,Yamaguchi N

AKAP95 regulates splicing through scaffolding RNAs and RNA processing factors.
Nature communications713347(2016 Nov)
Hu J,Khodadadi-Jamayran A,Mao M,Shah K,Yang Z,Nasim MT,Wang Z,Jiang H

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AKAP8 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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