Akap8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Akap8
Entrez Gene ID	116633
Full Name	A-kinase anchoring protein 8
Synonyms	Akap95
General protein information	Preferred Names A-kinase anchoring protein 8 Names A-kinase anchor protein 8 A kinase (PRKA) anchor protein 8 A-kinase anchor protein 95 kDa AKAP 95 AKAP-8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11
Summary	scaffolding protein that is involved in targeting type II cAMP-dependent protein kinase for cAMP-responsive nuclear events [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594573.1	XP_017450062.1	A-kinase anchor protein 8 isoform X2
XM_006241047.3	XP_006241109.1	A-kinase anchor protein 8 isoform X1
XM_017594574.1	XP_017450063.1	A-kinase anchor protein 8 isoform X2
NM_053855.1	NP_446307.1	A-kinase anchor protein 8

Pathways from BioSystems

Homologies

Bos taurus (cattle)	AKAP8	NP_001095542.1
Pan troglodytes (chimpanzee)	AKAP8	XP_001159349.2
Canis lupus familiaris (dog)	AKAP8	NP_001007124.1
Mus musculus (house mouse)	Akap8	NP_062748.2
Homo sapiens (human)	AKAP8	NP_005849.1
	AKAP8	XP_001112993.2
Rattus norvegicus (Norway rat)	Akap8	NP_446307.1
Gallus gallus (chicken)	AKAP8	XP_004950089.1

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Suppression of LPS-induced TNF-alpha production in macrophages by cAMP is mediated by PKA-AKAP95-p105.
Wall EA, Zavzavadjian JR, Chang MS, Randhawa B, Zhu X, Hsueh RC, Liu J, Driver A, Bao XR, Sternweis PC, Simon MI, Fraser ID
Science signaling2(75)ra28(2009 Jun)

Cyclic AMP and AKAP-mediated targeting of protein kinase A regulates lactate dehydrogenase subunit A mRNA stability.
Jungmann RA, Kiryukhina O
The Journal of biological chemistry280(26)25170-7(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

new insights into how AKAP95 orchestrates cell cycle progression.
Title: Localization and retention of p90 ribosomal S6 kinase 1 in the nucleus: implications for its function.

Used cell permeable peptide to disrupt endogenous protein kinase A R2alpha (pkar2a)-AKAP95 complexes.
Title: Cell-permeable peptide-based disruption of endogenous PKA-AKAP complexes: a tool for studying the molecular roles of AKAP-mediated PKA subcellular anchoring.

lactate dehydrogenase subunit A mRNA stability is regulated by Cyclic AMP and AKAP-mediated targeting of protein kinase A
Title: Cyclic AMP and AKAP-mediated targeting of protein kinase A regulates lactate dehydrogenase subunit A mRNA stability.

The following Akap8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Akap8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa29944	XM_006241047.3 Latest version!	Rattus norvegicus A-kinase anchoring protein 8 (Akap8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa10870	NM_053855.1 Latest version!	Rattus norvegicus A-kinase anchoring protein 8 (Akap8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa61836	XM_017594573.1 Latest version!	Rattus norvegicus A-kinase anchoring protein 8 (Akap8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa61836	XM_017594574.1 Latest version!	Rattus norvegicus A-kinase anchoring protein 8 (Akap8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Akap8 ( XM_006241047.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241109.1 Rattus norvegicus A-kinase anchoring protein 8 (Akap8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa29944
Clone ID Related Accession (Same CDS sequence)	XM_006241047.3
Accession Version	XM_006241047.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241047.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGTGG CCTCTTTTGC CCCAGAGCCA TGCACCGACA ACTCTGACTC GCTCATTGCC 
AAGATCAATC AACGTTTGGA CATGTTGTCC AAGGAAGGAG GCAGGGGTGG GATCAGCAGC 
GGTGGGGAGG GCATGCAGGA CCGAGACAGC TCCTTCCGCT TCCAGCCATA TGAGTCCTAC 
GACTCCAGGC CCTGTATGCC TGAGCATACT CCCTACCGCC CCAGCTACAG TTACGATTAT 
GACTTTGACT TGGGAACTGA CCGCAATGGT AGTTTTGGCG GGACATTCAA CGACTGTCGG 
GACCCAACCC CAGAGCGAGG CGCCCTTGAT GGTTTCTTAA GGGGCCGGGG CCAGGGCCGC 
TTCCAGGACC GGAGCAACTC GAGCACCTTC ATACGTAGTG ACCCCTTCAT GCCACCCTCA 
GCCTCCTCAG AGCCCTTATC CACCACCTGG AGTGAGCTGA ACTACATGGG TGGACGTGGT 
CTAGGTGGGC CCTCCACCAA CAGGCCGCCT CCTTCCCTCT TCTCCCAGTC CATGGCCCCT 
GACTACAGCA TGATGGGCAT GCAGGGGGTG GGCGGTTTTG GTGGCACCAT GCCTTATGGA 
TGTGGCCGGT CCCAGACTCG GATACGGGAT TGGCCCAGAA GGAGGGGGTT TGAACGCTTT 
GGACCAGACA ACATGGGCAG GAAGCGGAAG CCGTTTCCAT TGTATGAAGA ACCTGATGCC 
AAGCTGGCCC GTGCTGACAG TGAAGGAGAC CTCTCTGAAA ACGATGATGG AGCTGGTGAC 
TTACGGTCAG GAGATGAAGA ATTTAGGGGG GAGGACGACC TCTGTGACTC CCGGAAGCAG 
AGAGGAGAAA AGGAGGACGA GGATGAGGAT GTGAAGAAGA GACGGGAGAA GCAAAGGAGG 
AGAGATCGGA TGCGGGACCG AGCAGCTGAC AGGATTCAGT TTGCCTGTTC TGTGTGCAAA 
TTTCGTAGCT TTGAAGATGA AGAAATCCAA AAGCATCTGC AAAGTAAATT TCATAAAGAG 
ACCTTGCGGT TTATAAGTAC CAAACTACCT GACAAGACAG TAGAATTTCT CCAGGAGTAT 
ATCATAAACA GGAATAAGAA AATTGAGAAA CGGCGTCAGG AGTTGTTGGA GAAGGAAAGC 
CCTAAACCCA AACCAGATCC ATTCAAAGGG ATTGGCCAGG AGCATTTCTT CAAAAGGATT 
GAAGCCGCAC ACTGCCTGGC CTGTGACATG CTGATTCCTG CACAGCACCA GCTCCTGCAG 
CGGCATCTGC ACTCTGTGGA CCATAACCAT AATCGAAGGT TGGCTGCTGA ACAATTCAAG 
AAAACAAGTC TCCATGTGGC TAAGAGTGTT CTGAACAACA AGCATATAGT GAAGATGTTA 
GAAAAATACC TCAAGGGCGA GGATCCTTTT GTCAATGAAA CTGCTGATCT TGAGACAGAA 
GGAGATGAGA ACTTAGGAGA GGAGAAGGAG ACACCAGAGG AGGTAGCTGC GGAAGTCTTA 
GCAGAGGTGA TCACAGCAGC GGTGAAGGCT GTAGAGGGGG ATGGAGAACC AGCTGCAGAG 
CATAGTGACG TCCTAGCTGA AGTGGAAGGG CCTGTGGACA CCGCCGAGGC TGGTAGTGAC 
TCCCACACTG GAAAGCTGCT AGAAGAACAG ACCTGTGAAA CAGCATCTGA AACCAGGAAC 
ATGGAAGACA TGGCCAGAGG TGAGGCTGCT GAGGCCAGAA ATGAAGCAGC TGTGCCAGCA 
GCAGCCGCCG GAAGCCCAGT ACCTGTCATA GCCATCCCAG GAATCCTGGA AGATGAGCTG 
GAACAAACTG ATGCAGAGGC CAAAGATACT CCCACAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241109.1
CDS	472..2313
Translation	MHVASFAPEPCTDNSDSLIAKINQRLDMLSKEGGRGGISSGGEGMQDRDSSFRFQPYESYDSRPCMPEHTPYRPSYSYDYDFDLGTDRNGSFGGTFNDCRDPTPERGALDGFLRGRGQGRFQDRSNSSTFIRSDPFMPPSASSEPLSTTWSELNYMGGRGLGGPSTNRPPPSLFSQSMAPDYSMMGMQGVGGFGGTMPYGCGRSQTRIRDWPRRRGFERFGPDNMGRKRKPFPLYEEPDAKLARADSEGDLSENDDGAGDLRSGDEEFRGEDDLCDSRKQRGEKEDEDEDVKKRREKQRRRDRMRDRAADRIQFACSVCKFRSFEDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIINRNKKIEKRRQELLEKESPKPKPDPFKGIGQEHFFKRIEAAHCLACDMLIPAQHQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNKHIVKMLEKYLKGEDPFVNETADLETEGDENLGEEKETPEEVAAEVLAEVITAAVKAVEGDGEPAAEHSDVLAEVEGPVDTAEAGSDSHTGKLLEEQTCETASETRNMEDMARGEAAEARNEAAVPAAAAGSPVPVIAIPGILEDELEQTDAEAKDTPTE

Target ORF information:

RefSeq Version	XM_006241047.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus A-kinase anchoring protein 8 (Akap8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241047.3

ORF Insert Sequence:

ATGCATGTGG CCTCTTTTGC CCCAGAGCCA TGCACCGACA ACTCTGACTC GCTCATTGCC 
AAGATCAATC AACGTTTGGA CATGTTGTCC AAGGAAGGAG GCAGGGGTGG GATCAGCAGC 
GGTGGGGAGG GCATGCAGGA CCGAGACAGC TCCTTCCGCT TCCAGCCATA TGAGTCCTAC 
GACTCCAGGC CCTGTATGCC TGAGCATACT CCCTACCGCC CCAGCTACAG TTACGATTAT 
GACTTTGACT TGGGAACTGA CCGCAATGGT AGTTTTGGCG GGACATTCAA CGACTGTCGG 
GACCCAACCC CAGAGCGAGG CGCCCTTGAT GGTTTCTTAA GGGGCCGGGG CCAGGGCCGC 
TTCCAGGACC GGAGCAACTC GAGCACCTTC ATACGTAGTG ACCCCTTCAT GCCACCCTCA 
GCCTCCTCAG AGCCCTTATC CACCACCTGG AGTGAGCTGA ACTACATGGG TGGACGTGGT 
CTAGGTGGGC CCTCCACCAA CAGGCCGCCT CCTTCCCTCT TCTCCCAGTC CATGGCCCCT 
GACTACAGCA TGATGGGCAT GCAGGGGGTG GGCGGTTTTG GTGGCACCAT GCCTTATGGA 
TGTGGCCGGT CCCAGACTCG GATACGGGAT TGGCCCAGAA GGAGGGGGTT TGAACGCTTT 
GGACCAGACA ACATGGGCAG GAAGCGGAAG CCGTTTCCAT TGTATGAAGA ACCTGATGCC 
AAGCTGGCCC GTGCTGACAG TGAAGGAGAC CTCTCTGAAA ACGATGATGG AGCTGGTGAC 
TTACGGTCAG GAGATGAAGA ATTTAGGGGG GAGGACGACC TCTGTGACTC CCGGAAGCAG 
AGAGGAGAAA AGGAGGACGA GGATGAGGAT GTGAAGAAGA GACGGGAGAA GCAAAGGAGG 
AGAGATCGGA TGCGGGACCG AGCAGCTGAC AGGATTCAGT TTGCCTGTTC TGTGTGCAAA 
TTTCGTAGCT TTGAAGATGA AGAAATCCAA AAGCATCTGC AAAGTAAATT TCATAAAGAG 
ACCTTGCGGT TTATAAGTAC CAAACTACCT GACAAGACAG TAGAATTTCT CCAGGAGTAT 
ATCATAAACA GGAATAAGAA AATTGAGAAA CGGCGTCAGG AGTTGTTGGA GAAGGAAAGC 
CCTAAACCCA AACCAGATCC ATTCAAAGGG ATTGGCCAGG AGCATTTCTT CAAAAGGATT 
GAAGCCGCAC ACTGCCTGGC CTGTGACATG CTGATTCCTG CACAGCACCA GCTCCTGCAG 
CGGCATCTGC ACTCTGTGGA CCATAACCAT AATCGAAGGT TGGCTGCTGA ACAATTCAAG 
AAAACAAGTC TCCATGTGGC TAAGAGTGTT CTGAACAACA AGCATATAGT GAAGATGTTA 
GAAAAATACC TCAAGGGCGA GGATCCTTTT GTCAATGAAA CTGCTGATCT TGAGACAGAA 
GGAGATGAGA ACTTAGGAGA GGAGAAGGAG ACACCAGAGG AGGTAGCTGC GGAAGTCTTA 
GCAGAGGTGA TCACAGCAGC GGTGAAGGCT GTAGAGGGGG ATGGAGAACC AGCTGCAGAG 
CATAGTGACG TCCTAGCTGA AGTGGAAGGG CCTGTGGACA CCGCCGAGGC TGGTAGTGAC 
TCCCACACTG GAAAGCTGCT AGAAGAACAG ACCTGTGAAA CAGCATCTGA AACCAGGAAC 
ATGGAAGACA TGGCCAGAGG TGAGGCTGCT GAGGCCAGAA ATGAAGCAGC TGTGCCAGCA 
GCAGCCGCCG GAAGCCCAGT ACCTGTCATA GCCATCCCAG GAATCCTGGA AGATGAGCTG 
GAACAAACTG ATGCAGAGGC CAAAGATACT CCCACAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akap8 ( NM_053855.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446307.1 Rattus norvegicus A-kinase anchoring protein 8 (Akap8), mRNA.

Price & Availability↑

CloneID	ORa10870
Clone ID Related Accession (Same CDS sequence)	NM_053855.1
Accession Version	NM_053855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein 8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166580.1. On or before Sep 7, 2008 this sequence version replaced XM_343176.3, XM_001074708.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC166580.1, U01914.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCAAA GCTACGGAGG TTATGGGGCA TGGAGTGCTG GACCTGCCAA CACCCAGGGT 
ACATATGGAA GTGGTGTGGC CAGCTGGCAA GGTTATGAAA ACTACAGCTA CTACAATGCC 
CAGAACACCA GTGTCCCTAC AGGAACACCC TATAGTTATG GCCCAGCCTC GTGGGAGGCC 
ACCAAGGCCA GTGATGGTGG CCTGGCAGCT GGGAGTTCTG CTATGCATGT GGCCTCTTTT 
GCCCCAGAGC CATGCACCGA CAACTCTGAC TCGCTCATTG CCAAGATCAA TCAACGTTTG 
GACATGTTGT CCAAGGAAGG AGGCAGGGGT GGGATCAGCA GCGGTGGGGA GGGCATGCAG 
GACCGAGACA GCTCCTTCCG CTTCCAGCCA TATGAGTCCT ACGACTCCAG GCCCTGTATG 
CCTGAGCATA CTCCCTACCG CCCCAGCTAC AGTTACGATT ATGACTTTGA CTTGGGAACT 
GACCGCAATG GTAGTTTTGG CGGGACATTC AACGACTGTC GGGACCCAAC CCCAGAGCGA 
GGCGCCCTTG ATGGTTTCTT AAGGGGCCGG GGCCAGGGCC GCTTCCAGGA CCGGAGCAAC 
TCGAGCACCT TCATACGTAG TGACCCCTTC ATGCCACCCT CAGCCTCCTC AGAGCCCTTA 
TCCACCACCT GGAGTGAGCT GAACTACATG GGTGGACGTG GTCTAGGTGG GCCCTCCACC 
AACAGGCCGC CTCCTTCCCT CTTCTCCCAG TCCATGGCCC CTGACTACAG CATGATGGGC 
ATGCAGGGGG TGGGCGGTTT TGGTGGCACC ATGCCTTATG GATGTGGCCG GTCCCAGACT 
CGGATACGGG ATTGGCCCAG AAGGAGGGGG TTTGAACGCT TTGGACCAGA CAACATGGGC 
AGGAAGCGGA AGCCGTTTCC ATTGTATGAA GAACCTGATG CCAAGCTGGC CCGTGCTGAC 
AGTGAAGGAG ACCTCTCTGA AAACGATGAT GGAGCTGGTG ACTTACGGTC AGGAGATGAA 
GAATTTAGGG GGGAGGACGA CCTCTGTGAC TCCCGGAAGC AGAGAGGAGA AAAGGAGGAC 
GAGGATGAGG ATGTGAAGAA GAGACGGGAG AAGCAAAGGA GGAGAGATCG GATGCGGGAC 
CGAGCAGCTG ACAGGATTCA GTTTGCCTGT TCTGTGTGCA AATTTCGTAG CTTTGAAGAT 
GAAGAAATCC AAAAGCATCT GCAAAGTAAA TTTCATAAAG AGACCTTGCG GTTTATAAGT 
ACCAAACTAC CTGACAAGAC AGTAGAATTT CTCCAGGAGT ATATCATAAA CAGGAATAAG 
AAAATTGAGA AACGGCGTCA GGAGTTGTTG GAGAAGGAAA GCCCTAAACC CAAACCAGAT 
CCATTCAAAG GGATTGGCCA GGAGCATTTC TTCAAAAGGA TTGAAGCCGC ACACTGCCTG 
GCCTGTGACA TGCTGATTCC TGCACAGCAC CAGCTCCTGC AGCGGCATCT GCACTCTGTG 
GACCATAACC ATAATCGAAG GTTGGCTGCT GAACAATTCA AGAAAACAAG TCTCCATGTG 
GCTAAGAGTG TTCTGAACAA CAAGCATATA GTGAAGATGT TAGAAAAATA CCTCAAGGGC 
GAGGATCCTT TTGTCAATGA AACTGCTGAT CTTGAGACAG AAGGAGATGA GAACTTAGGA 
GAGGAGAAGG AGACACCAGA GGAGGTAGCT GCGGAAGTCT TAGCAGAGGT GATCACAGCA 
GCGGTGAAGG CTGTAGAGGG GGATGGAGAA CCAGCTGCAG AGCATAGTGA CGTCCTAGCT 
GAAGTGGAAG GGCCTGTGGA CACCGCCGAG GCTGGTAGTG ACTCCCACAC TGGAAAGCTG 
CTAGAAGAAC AGACCTGTGA AACAGCATCT GAAACCAGGA ACATGGAAGA CATGGCCAGA 
GGTGAGGCTG CTGAGGCCAG AAATGAAGCA GCTGTGCCAG CAGCAGCCGC CGGAAGCCCA 
GTACCTGTCA TAGCCATCCC AGGAATCCTG GAAGATGAGC TGGAACAAAC TGATGCAGAG 
GCCAAAGATA CTCCCACAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446307.1
CDS	26..2089
Translation	MEQSYGGYGAWSAGPANTQGTYGSGVASWQGYENYSYYNAQNTSVPTGTPYSYGPASWEATKASDGGLAAGSSAMHVASFAPEPCTDNSDSLIAKINQRLDMLSKEGGRGGISSGGEGMQDRDSSFRFQPYESYDSRPCMPEHTPYRPSYSYDYDFDLGTDRNGSFGGTFNDCRDPTPERGALDGFLRGRGQGRFQDRSNSSTFIRSDPFMPPSASSEPLSTTWSELNYMGGRGLGGPSTNRPPPSLFSQSMAPDYSMMGMQGVGGFGGTMPYGCGRSQTRIRDWPRRRGFERFGPDNMGRKRKPFPLYEEPDAKLARADSEGDLSENDDGAGDLRSGDEEFRGEDDLCDSRKQRGEKEDEDEDVKKRREKQRRRDRMRDRAADRIQFACSVCKFRSFEDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIINRNKKIEKRRQELLEKESPKPKPDPFKGIGQEHFFKRIEAAHCLACDMLIPAQHQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNKHIVKMLEKYLKGEDPFVNETADLETEGDENLGEEKETPEEVAAEVLAEVITAAVKAVEGDGEPAAEHSDVLAEVEGPVDTAEAGSDSHTGKLLEEQTCETASETRNMEDMARGEAAEARNEAAVPAAAAGSPVPVIAIPGILEDELEQTDAEAKDTPTE

Target ORF information:

RefSeq Version	NM_053855.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus A-kinase anchoring protein 8 (Akap8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053855.1

ORF Insert Sequence:

ATGGAGCAAA GCTACGGAGG TTATGGGGCA TGGAGTGCTG GACCTGCCAA CACCCAGGGT 
ACATATGGAA GTGGTGTGGC CAGCTGGCAA GGTTATGAAA ACTACAGCTA CTACAATGCC 
CAGAACACCA GTGTCCCTAC AGGAACACCC TATAGTTATG GCCCAGCCTC GTGGGAGGCC 
ACCAAGGCCA GTGATGGTGG CCTGGCAGCT GGGAGTTCTG CTATGCATGT GGCCTCTTTT 
GCCCCAGAGC CATGCACCGA CAACTCTGAC TCGCTCATTG CCAAGATCAA TCAACGTTTG 
GACATGTTGT CCAAGGAAGG AGGCAGGGGT GGGATCAGCA GCGGTGGGGA GGGCATGCAG 
GACCGAGACA GCTCCTTCCG CTTCCAGCCA TATGAGTCCT ACGACTCCAG GCCCTGTATG 
CCTGAGCATA CTCCCTACCG CCCCAGCTAC AGTTACGATT ATGACTTTGA CTTGGGAACT 
GACCGCAATG GTAGTTTTGG CGGGACATTC AACGACTGTC GGGACCCAAC CCCAGAGCGA 
GGCGCCCTTG ATGGTTTCTT AAGGGGCCGG GGCCAGGGCC GCTTCCAGGA CCGGAGCAAC 
TCGAGCACCT TCATACGTAG TGACCCCTTC ATGCCACCCT CAGCCTCCTC AGAGCCCTTA 
TCCACCACCT GGAGTGAGCT GAACTACATG GGTGGACGTG GTCTAGGTGG GCCCTCCACC 
AACAGGCCGC CTCCTTCCCT CTTCTCCCAG TCCATGGCCC CTGACTACAG CATGATGGGC 
ATGCAGGGGG TGGGCGGTTT TGGTGGCACC ATGCCTTATG GATGTGGCCG GTCCCAGACT 
CGGATACGGG ATTGGCCCAG AAGGAGGGGG TTTGAACGCT TTGGACCAGA CAACATGGGC 
AGGAAGCGGA AGCCGTTTCC ATTGTATGAA GAACCTGATG CCAAGCTGGC CCGTGCTGAC 
AGTGAAGGAG ACCTCTCTGA AAACGATGAT GGAGCTGGTG ACTTACGGTC AGGAGATGAA 
GAATTTAGGG GGGAGGACGA CCTCTGTGAC TCCCGGAAGC AGAGAGGAGA AAAGGAGGAC 
GAGGATGAGG ATGTGAAGAA GAGACGGGAG AAGCAAAGGA GGAGAGATCG GATGCGGGAC 
CGAGCAGCTG ACAGGATTCA GTTTGCCTGT TCTGTGTGCA AATTTCGTAG CTTTGAAGAT 
GAAGAAATCC AAAAGCATCT GCAAAGTAAA TTTCATAAAG AGACCTTGCG GTTTATAAGT 
ACCAAACTAC CTGACAAGAC AGTAGAATTT CTCCAGGAGT ATATCATAAA CAGGAATAAG 
AAAATTGAGA AACGGCGTCA GGAGTTGTTG GAGAAGGAAA GCCCTAAACC CAAACCAGAT 
CCATTCAAAG GGATTGGCCA GGAGCATTTC TTCAAAAGGA TTGAAGCCGC ACACTGCCTG 
GCCTGTGACA TGCTGATTCC TGCACAGCAC CAGCTCCTGC AGCGGCATCT GCACTCTGTG 
GACCATAACC ATAATCGAAG GTTGGCTGCT GAACAATTCA AGAAAACAAG TCTCCATGTG 
GCTAAGAGTG TTCTGAACAA CAAGCATATA GTGAAGATGT TAGAAAAATA CCTCAAGGGC 
GAGGATCCTT TTGTCAATGA AACTGCTGAT CTTGAGACAG AAGGAGATGA GAACTTAGGA 
GAGGAGAAGG AGACACCAGA GGAGGTAGCT GCGGAAGTCT TAGCAGAGGT GATCACAGCA 
GCGGTGAAGG CTGTAGAGGG GGATGGAGAA CCAGCTGCAG AGCATAGTGA CGTCCTAGCT 
GAAGTGGAAG GGCCTGTGGA CACCGCCGAG GCTGGTAGTG ACTCCCACAC TGGAAAGCTG 
CTAGAAGAAC AGACCTGTGA AACAGCATCT GAAACCAGGA ACATGGAAGA CATGGCCAGA 
GGTGAGGCTG CTGAGGCCAG AAATGAAGCA GCTGTGCCAG CAGCAGCCGC CGGAAGCCCA 
GTACCTGTCA TAGCCATCCC AGGAATCCTG GAAGATGAGC TGGAACAAAC TGATGCAGAG 
GCCAAAGATA CTCCCACAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akap8 ( XM_017594573.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450062.1 Rattus norvegicus A-kinase anchoring protein 8 (Akap8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa61836
Clone ID Related Accession (Same CDS sequence)	XM_017594573.1 , XM_017594574.1
Accession Version	XM_017594573.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGCT GGACCTGCCA ACACCCAGGC TCCTTCCGCT TCCAGCCATA TGAGTCCTAC 
GACTCCAGGC CCTGTATGCC TGAGCATACT CCCTACCGCC CCAGCTACAG TTACGATTAT 
GACTTTGACT TGGGAACTGA CCGCAATGGT AGTTTTGGCG GGACATTCAA CGACTGTCGG 
GACCCAACCC CAGAGCGAGG CGCCCTTGAT GGTTTCTTAA GGGGCCGGGG CCAGGGCCGC 
TTCCAGGACC GGAGCAACTC GAGCACCTTC ATACGTAGTG ACCCCTTCAT GCCACCCTCA 
GCCTCCTCAG AGCCCTTATC CACCACCTGG AGTGAGCTGA ACTACATGGG TGGACGTGGT 
CTAGGTGGGC CCTCCACCAA CAGGCCGCCT CCTTCCCTCT TCTCCCAGTC CATGGCCCCT 
GACTACAGCA TGATGGGCAT GCAGGGGGTG GGCGGTTTTG GTGGCACCAT GCCTTATGGA 
TGTGGCCGGT CCCAGACTCG GATACGGGAT TGGCCCAGAA GGAGGGGGTT TGAACGCTTT 
GGACCAGACA ACATGGGCAG GAAGCGGAAG CCGTTTCCAT TGTATGAAGA ACCTGATGCC 
AAGCTGGCCC GTGCTGACAG TGAAGGAGAC CTCTCTGAAA ACGATGATGG AGCTGGTGAC 
TTACGGTCAG GAGATGAAGA ATTTAGGGGG GAGGACGACC TCTGTGACTC CCGGAAGCAG 
AGAGGAGAAA AGGAGGACGA GGATGAGGAT GTGAAGAAGA GACGGGAGAA GCAAAGGAGG 
AGAGATCGGA TGCGGGACCG AGCAGCTGAC AGGATTCAGT TTGCCTGTTC TGTGTGCAAA 
TTTCGTAGCT TTGAAGATGA AGAAATCCAA AAGCATCTGC AAAGTAAATT TCATAAAGAG 
ACCTTGCGGT TTATAAGTAC CAAACTACCT GACAAGACAG TAGAATTTCT CCAGGAGTAT 
ATCATAAACA GGAATAAGAA AATTGAGAAA CGGCGTCAGG AGTTGTTGGA GAAGGAAAGC 
CCTAAACCCA AACCAGATCC ATTCAAAGGG ATTGGCCAGG AGCATTTCTT CAAAAGGATT 
GAAGCCGCAC ACTGCCTGGC CTGTGACATG CTGATTCCTG CACAGCACCA GCTCCTGCAG 
CGGCATCTGC ACTCTGTGGA CCATAACCAT AATCGAAGGT TGGCTGCTGA ACAATTCAAG 
AAAACAAGTC TCCATGTGGC TAAGAGTGTT CTGAACAACA AGCATATAGT GAAGATGTTA 
GAAAAATACC TCAAGGGCGA GGATCCTTTT GTCAATGAAA CTGCTGATCT TGAGACAGAA 
GGAGATGAGA ACTTAGGAGA GGAGAAGGAG ACACCAGAGG AGGTAGCTGC GGAAGTCTTA 
GCAGAGGTGA TCACAGCAGC GGTGAAGGCT GTAGAGGGGG ATGGAGAACC AGCTGCAGAG 
CATAGTGACG TCCTAGCTGA AGTGGAAGGG CCTGTGGACA CCGCCGAGGC TGGTAGTGAC 
TCCCACACTG GAAAGCTGCT AGAAGAACAG ACCTGTGAAA CAGCATCTGA AACCAGGAAC 
ATGGAAGACA TGGCCAGAGG TGAGGCTGCT GAGGCCAGAA ATGAAGCAGC TGTGCCAGCA 
GCAGCCGCCG GAAGCCCAGT ACCTGTCATA GCCATCCCAG GAATCCTGGA AGATGAGCTG 
GAACAAACTG ATGCAGAGGC CAAAGATACT CCCACAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450062.1
CDS	155..1876
Translation	MECWTCQHPGSFRFQPYESYDSRPCMPEHTPYRPSYSYDYDFDLGTDRNGSFGGTFNDCRDPTPERGALDGFLRGRGQGRFQDRSNSSTFIRSDPFMPPSASSEPLSTTWSELNYMGGRGLGGPSTNRPPPSLFSQSMAPDYSMMGMQGVGGFGGTMPYGCGRSQTRIRDWPRRRGFERFGPDNMGRKRKPFPLYEEPDAKLARADSEGDLSENDDGAGDLRSGDEEFRGEDDLCDSRKQRGEKEDEDEDVKKRREKQRRRDRMRDRAADRIQFACSVCKFRSFEDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIINRNKKIEKRRQELLEKESPKPKPDPFKGIGQEHFFKRIEAAHCLACDMLIPAQHQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNKHIVKMLEKYLKGEDPFVNETADLETEGDENLGEEKETPEEVAAEVLAEVITAAVKAVEGDGEPAAEHSDVLAEVEGPVDTAEAGSDSHTGKLLEEQTCETASETRNMEDMARGEAAEARNEAAVPAAAAGSPVPVIAIPGILEDELEQTDAEAKDTPTE

Target ORF information:

RefSeq Version	XM_017594573.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus A-kinase anchoring protein 8 (Akap8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594573.1

ORF Insert Sequence:

ATGGAGTGCT GGACCTGCCA ACACCCAGGC TCCTTCCGCT TCCAGCCATA TGAGTCCTAC 
GACTCCAGGC CCTGTATGCC TGAGCATACT CCCTACCGCC CCAGCTACAG TTACGATTAT 
GACTTTGACT TGGGAACTGA CCGCAATGGT AGTTTTGGCG GGACATTCAA CGACTGTCGG 
GACCCAACCC CAGAGCGAGG CGCCCTTGAT GGTTTCTTAA GGGGCCGGGG CCAGGGCCGC 
TTCCAGGACC GGAGCAACTC GAGCACCTTC ATACGTAGTG ACCCCTTCAT GCCACCCTCA 
GCCTCCTCAG AGCCCTTATC CACCACCTGG AGTGAGCTGA ACTACATGGG TGGACGTGGT 
CTAGGTGGGC CCTCCACCAA CAGGCCGCCT CCTTCCCTCT TCTCCCAGTC CATGGCCCCT 
GACTACAGCA TGATGGGCAT GCAGGGGGTG GGCGGTTTTG GTGGCACCAT GCCTTATGGA 
TGTGGCCGGT CCCAGACTCG GATACGGGAT TGGCCCAGAA GGAGGGGGTT TGAACGCTTT 
GGACCAGACA ACATGGGCAG GAAGCGGAAG CCGTTTCCAT TGTATGAAGA ACCTGATGCC 
AAGCTGGCCC GTGCTGACAG TGAAGGAGAC CTCTCTGAAA ACGATGATGG AGCTGGTGAC 
TTACGGTCAG GAGATGAAGA ATTTAGGGGG GAGGACGACC TCTGTGACTC CCGGAAGCAG 
AGAGGAGAAA AGGAGGACGA GGATGAGGAT GTGAAGAAGA GACGGGAGAA GCAAAGGAGG 
AGAGATCGGA TGCGGGACCG AGCAGCTGAC AGGATTCAGT TTGCCTGTTC TGTGTGCAAA 
TTTCGTAGCT TTGAAGATGA AGAAATCCAA AAGCATCTGC AAAGTAAATT TCATAAAGAG 
ACCTTGCGGT TTATAAGTAC CAAACTACCT GACAAGACAG TAGAATTTCT CCAGGAGTAT 
ATCATAAACA GGAATAAGAA AATTGAGAAA CGGCGTCAGG AGTTGTTGGA GAAGGAAAGC 
CCTAAACCCA AACCAGATCC ATTCAAAGGG ATTGGCCAGG AGCATTTCTT CAAAAGGATT 
GAAGCCGCAC ACTGCCTGGC CTGTGACATG CTGATTCCTG CACAGCACCA GCTCCTGCAG 
CGGCATCTGC ACTCTGTGGA CCATAACCAT AATCGAAGGT TGGCTGCTGA ACAATTCAAG 
AAAACAAGTC TCCATGTGGC TAAGAGTGTT CTGAACAACA AGCATATAGT GAAGATGTTA 
GAAAAATACC TCAAGGGCGA GGATCCTTTT GTCAATGAAA CTGCTGATCT TGAGACAGAA 
GGAGATGAGA ACTTAGGAGA GGAGAAGGAG ACACCAGAGG AGGTAGCTGC GGAAGTCTTA 
GCAGAGGTGA TCACAGCAGC GGTGAAGGCT GTAGAGGGGG ATGGAGAACC AGCTGCAGAG 
CATAGTGACG TCCTAGCTGA AGTGGAAGGG CCTGTGGACA CCGCCGAGGC TGGTAGTGAC 
TCCCACACTG GAAAGCTGCT AGAAGAACAG ACCTGTGAAA CAGCATCTGA AACCAGGAAC 
ATGGAAGACA TGGCCAGAGG TGAGGCTGCT GAGGCCAGAA ATGAAGCAGC TGTGCCAGCA 
GCAGCCGCCG GAAGCCCAGT ACCTGTCATA GCCATCCCAG GAATCCTGGA AGATGAGCTG 
GAACAAACTG ATGCAGAGGC CAAAGATACT CCCACAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akap8 ( XM_017594574.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450063.1 Rattus norvegicus A-kinase anchoring protein 8 (Akap8), transcript variant X3, mRNA.

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CloneID	ORa61836
Clone ID Related Accession (Same CDS sequence)	XM_017594573.1 , XM_017594574.1
Accession Version	XM_017594574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGCT GGACCTGCCA ACACCCAGGC TCCTTCCGCT TCCAGCCATA TGAGTCCTAC 
GACTCCAGGC CCTGTATGCC TGAGCATACT CCCTACCGCC CCAGCTACAG TTACGATTAT 
GACTTTGACT TGGGAACTGA CCGCAATGGT AGTTTTGGCG GGACATTCAA CGACTGTCGG 
GACCCAACCC CAGAGCGAGG CGCCCTTGAT GGTTTCTTAA GGGGCCGGGG CCAGGGCCGC 
TTCCAGGACC GGAGCAACTC GAGCACCTTC ATACGTAGTG ACCCCTTCAT GCCACCCTCA 
GCCTCCTCAG AGCCCTTATC CACCACCTGG AGTGAGCTGA ACTACATGGG TGGACGTGGT 
CTAGGTGGGC CCTCCACCAA CAGGCCGCCT CCTTCCCTCT TCTCCCAGTC CATGGCCCCT 
GACTACAGCA TGATGGGCAT GCAGGGGGTG GGCGGTTTTG GTGGCACCAT GCCTTATGGA 
TGTGGCCGGT CCCAGACTCG GATACGGGAT TGGCCCAGAA GGAGGGGGTT TGAACGCTTT 
GGACCAGACA ACATGGGCAG GAAGCGGAAG CCGTTTCCAT TGTATGAAGA ACCTGATGCC 
AAGCTGGCCC GTGCTGACAG TGAAGGAGAC CTCTCTGAAA ACGATGATGG AGCTGGTGAC 
TTACGGTCAG GAGATGAAGA ATTTAGGGGG GAGGACGACC TCTGTGACTC CCGGAAGCAG 
AGAGGAGAAA AGGAGGACGA GGATGAGGAT GTGAAGAAGA GACGGGAGAA GCAAAGGAGG 
AGAGATCGGA TGCGGGACCG AGCAGCTGAC AGGATTCAGT TTGCCTGTTC TGTGTGCAAA 
TTTCGTAGCT TTGAAGATGA AGAAATCCAA AAGCATCTGC AAAGTAAATT TCATAAAGAG 
ACCTTGCGGT TTATAAGTAC CAAACTACCT GACAAGACAG TAGAATTTCT CCAGGAGTAT 
ATCATAAACA GGAATAAGAA AATTGAGAAA CGGCGTCAGG AGTTGTTGGA GAAGGAAAGC 
CCTAAACCCA AACCAGATCC ATTCAAAGGG ATTGGCCAGG AGCATTTCTT CAAAAGGATT 
GAAGCCGCAC ACTGCCTGGC CTGTGACATG CTGATTCCTG CACAGCACCA GCTCCTGCAG 
CGGCATCTGC ACTCTGTGGA CCATAACCAT AATCGAAGGT TGGCTGCTGA ACAATTCAAG 
AAAACAAGTC TCCATGTGGC TAAGAGTGTT CTGAACAACA AGCATATAGT GAAGATGTTA 
GAAAAATACC TCAAGGGCGA GGATCCTTTT GTCAATGAAA CTGCTGATCT TGAGACAGAA 
GGAGATGAGA ACTTAGGAGA GGAGAAGGAG ACACCAGAGG AGGTAGCTGC GGAAGTCTTA 
GCAGAGGTGA TCACAGCAGC GGTGAAGGCT GTAGAGGGGG ATGGAGAACC AGCTGCAGAG 
CATAGTGACG TCCTAGCTGA AGTGGAAGGG CCTGTGGACA CCGCCGAGGC TGGTAGTGAC 
TCCCACACTG GAAAGCTGCT AGAAGAACAG ACCTGTGAAA CAGCATCTGA AACCAGGAAC 
ATGGAAGACA TGGCCAGAGG TGAGGCTGCT GAGGCCAGAA ATGAAGCAGC TGTGCCAGCA 
GCAGCCGCCG GAAGCCCAGT ACCTGTCATA GCCATCCCAG GAATCCTGGA AGATGAGCTG 
GAACAAACTG ATGCAGAGGC CAAAGATACT CCCACAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450063.1
CDS	279..2000
Translation	MECWTCQHPGSFRFQPYESYDSRPCMPEHTPYRPSYSYDYDFDLGTDRNGSFGGTFNDCRDPTPERGALDGFLRGRGQGRFQDRSNSSTFIRSDPFMPPSASSEPLSTTWSELNYMGGRGLGGPSTNRPPPSLFSQSMAPDYSMMGMQGVGGFGGTMPYGCGRSQTRIRDWPRRRGFERFGPDNMGRKRKPFPLYEEPDAKLARADSEGDLSENDDGAGDLRSGDEEFRGEDDLCDSRKQRGEKEDEDEDVKKRREKQRRRDRMRDRAADRIQFACSVCKFRSFEDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIINRNKKIEKRRQELLEKESPKPKPDPFKGIGQEHFFKRIEAAHCLACDMLIPAQHQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNKHIVKMLEKYLKGEDPFVNETADLETEGDENLGEEKETPEEVAAEVLAEVITAAVKAVEGDGEPAAEHSDVLAEVEGPVDTAEAGSDSHTGKLLEEQTCETASETRNMEDMARGEAAEARNEAAVPAAAAGSPVPVIAIPGILEDELEQTDAEAKDTPTE

Target ORF information:

RefSeq Version	XM_017594574.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus A-kinase anchoring protein 8 (Akap8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594574.1

ORF Insert Sequence:

ATGGAGTGCT GGACCTGCCA ACACCCAGGC TCCTTCCGCT TCCAGCCATA TGAGTCCTAC 
GACTCCAGGC CCTGTATGCC TGAGCATACT CCCTACCGCC CCAGCTACAG TTACGATTAT 
GACTTTGACT TGGGAACTGA CCGCAATGGT AGTTTTGGCG GGACATTCAA CGACTGTCGG 
GACCCAACCC CAGAGCGAGG CGCCCTTGAT GGTTTCTTAA GGGGCCGGGG CCAGGGCCGC 
TTCCAGGACC GGAGCAACTC GAGCACCTTC ATACGTAGTG ACCCCTTCAT GCCACCCTCA 
GCCTCCTCAG AGCCCTTATC CACCACCTGG AGTGAGCTGA ACTACATGGG TGGACGTGGT 
CTAGGTGGGC CCTCCACCAA CAGGCCGCCT CCTTCCCTCT TCTCCCAGTC CATGGCCCCT 
GACTACAGCA TGATGGGCAT GCAGGGGGTG GGCGGTTTTG GTGGCACCAT GCCTTATGGA 
TGTGGCCGGT CCCAGACTCG GATACGGGAT TGGCCCAGAA GGAGGGGGTT TGAACGCTTT 
GGACCAGACA ACATGGGCAG GAAGCGGAAG CCGTTTCCAT TGTATGAAGA ACCTGATGCC 
AAGCTGGCCC GTGCTGACAG TGAAGGAGAC CTCTCTGAAA ACGATGATGG AGCTGGTGAC 
TTACGGTCAG GAGATGAAGA ATTTAGGGGG GAGGACGACC TCTGTGACTC CCGGAAGCAG 
AGAGGAGAAA AGGAGGACGA GGATGAGGAT GTGAAGAAGA GACGGGAGAA GCAAAGGAGG 
AGAGATCGGA TGCGGGACCG AGCAGCTGAC AGGATTCAGT TTGCCTGTTC TGTGTGCAAA 
TTTCGTAGCT TTGAAGATGA AGAAATCCAA AAGCATCTGC AAAGTAAATT TCATAAAGAG 
ACCTTGCGGT TTATAAGTAC CAAACTACCT GACAAGACAG TAGAATTTCT CCAGGAGTAT 
ATCATAAACA GGAATAAGAA AATTGAGAAA CGGCGTCAGG AGTTGTTGGA GAAGGAAAGC 
CCTAAACCCA AACCAGATCC ATTCAAAGGG ATTGGCCAGG AGCATTTCTT CAAAAGGATT 
GAAGCCGCAC ACTGCCTGGC CTGTGACATG CTGATTCCTG CACAGCACCA GCTCCTGCAG 
CGGCATCTGC ACTCTGTGGA CCATAACCAT AATCGAAGGT TGGCTGCTGA ACAATTCAAG 
AAAACAAGTC TCCATGTGGC TAAGAGTGTT CTGAACAACA AGCATATAGT GAAGATGTTA 
GAAAAATACC TCAAGGGCGA GGATCCTTTT GTCAATGAAA CTGCTGATCT TGAGACAGAA 
GGAGATGAGA ACTTAGGAGA GGAGAAGGAG ACACCAGAGG AGGTAGCTGC GGAAGTCTTA 
GCAGAGGTGA TCACAGCAGC GGTGAAGGCT GTAGAGGGGG ATGGAGAACC AGCTGCAGAG 
CATAGTGACG TCCTAGCTGA AGTGGAAGGG CCTGTGGACA CCGCCGAGGC TGGTAGTGAC 
TCCCACACTG GAAAGCTGCT AGAAGAACAG ACCTGTGAAA CAGCATCTGA AACCAGGAAC 
ATGGAAGACA TGGCCAGAGG TGAGGCTGCT GAGGCCAGAA ATGAAGCAGC TGTGCCAGCA 
GCAGCCGCCG GAAGCCCAGT ACCTGTCATA GCCATCCCAG GAATCCTGGA AGATGAGCTG 
GAACAAACTG ATGCAGAGGC CAAAGATACT CCCACAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Suppression of LPS-induced TNF-alpha production in macrophages by cAMP is mediated by PKA-AKAP95-p105.
Science signaling2(75)ra28(2009 Jun)
Wall EA,Zavzavadjian JR,Chang MS,Randhawa B,Zhu X,Hsueh RC,Liu J,Driver A,Bao XR,Sternweis PC,Simon MI,Fraser ID

Cyclic AMP and AKAP-mediated targeting of protein kinase A regulates lactate dehydrogenase subunit A mRNA stability.
The Journal of biological chemistry280(26)25170-7(2005 Jul)
Jungmann RA,Kiryukhina O

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Akap8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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