KLRF2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	KLRF2
Entrez Gene ID	100431172
Full Name	killer cell lectin like receptor F2
Synonyms	NKp65
General protein information	Preferred Names killer cell lectin like receptor F2 Names killer cell lectin-like receptor subfamily F member 2 activating coreceptor NKp65 killer cell lectin-like receptor subfamily F, member 2 lectin-like receptor F2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p13.31

mRNA and Protein(s)

mRNA	Protein	Name
NM_001190765.1	NP_001177694.1	killer cell lectin-like receptor subfamily F member 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	KLRF2	XP_003433582.1
Pan troglodytes (chimpanzee)	KLRF2	XP_003313526.1
Homo sapiens (human)	KLRF2	NP_001177694.1
	LOC100428621	XP_002798522.1
Bos taurus (cattle)	KLRF2	XP_003586173.2

Gene Ontology
Bibliography

Related articles in PubMed

The Activating C-type Lectin-like Receptor NKp65 Signals through a Hemi-immunoreceptor Tyrosine-based Activation Motif (hemITAM) and Spleen Tyrosine Kinase (Syk).
Bauer B, Wotapek T, Z?ller T, Rutkowski E, Steinle A
The Journal of biological chemistry292(8)3213-3223(2017 02)

Key residues at the membrane-distal surface of KACL, but not glycosylation, determine the functional interaction of the keratinocyte-specific C-type lectin-like receptor KACL with its high-affinity receptor NKp65.
Bauer B, Spreu J, Rohe C, Vogler I, Steinle A
Immunology145(1)114-23(2015 May)

Structure of NKp65 bound to its keratinocyte ligand reveals basis for genetically linked recognition in natural killer gene complex.
Li Y, Wang Q, Chen S, Brown PH, Mariuzza RA
Proceedings of the National Academy of Sciences of the United States of America110(28)11505-10(2013 Jul)

Interaction of C-type lectin-like receptors NKp65 and KACL facilitates dedicated immune recognition of human keratinocytes.
Spreu J, Kuttruff S, Stejfova V, Dennehy KM, Schittek B, Steinle A
Proceedings of the National Academy of Sciences of the United States of America107(11)5100-5(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NKp65 utilizes a hemi-immunoreceptor tyrosine-based activation motif -like motif for cellular activation that requires Syk, although Syk appears not to be recruited to NKp65.
Title: The Activating C-type Lectin-like Receptor NKp65 Signals through a Hemi-immunoreceptor Tyrosine-based Activation Motif (hemITAM) and Spleen Tyrosine Kinase (Syk).

Key residues at the membrane-distal surface of KACL, but not glycosylation, determine the functional interaction of the keratinocyte-specific C-type lectin-like receptor KACL with its high-affinity receptor NKp65
Title: Key residues at the membrane-distal surface of KACL, but not glycosylation, determine the functional interaction of the keratinocyte-specific C-type lectin-like receptor KACL with its high-affinity receptor NKp65.

structure explains the exceptionally high affinity of the NKp65-KACL interaction compared with other cell-cell interaction pairs, which may compensate for the monomeric nature of NKp65 to achieve cell activation
Title: Structure of NKp65 bound to its keratinocyte ligand reveals basis for genetically linked recognition in natural killer gene complex.

Keratinocytes express KACL and are capable of stimulating NKp65-expressing cells in a KACL-dependent manner.
Title: Interaction of C-type lectin-like receptors NKp65 and KACL facilitates dedicated immune recognition of human keratinocytes.

The following KLRF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KLRF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu32349	NM_001190765.1 Latest version!	Homo sapiens killer cell lectin like receptor F2 (KLRF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KLRF2 ( NM_001190765.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177694.1 Homo sapiens killer cell lectin like receptor F2 (KLRF2), mRNA.

Price & Availability↑

CloneID	OHu32349
Clone ID Related Accession (Same CDS sequence)	NM_001190765.1
Accession Version	NM_001190765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-29
Organism	Homo sapiens(human)
Product	killer cell lectin-like receptor subfamily F member 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from GQ398770.1. ##Evidence-Data-START## Transcript exon combination :: GQ398770.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2163459 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000535540.1/ ENSP00000438244.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGAGAATG AAGATGGGTA TATGACGCTG AGTTTCAAGA ATCGTTGTAA ATCGAAGCAG 
AAATCTAAAG ATTTCTCCCT ATATCCACAA TATTATTGTC TTCTGCTCAT ATTTGGATGC 
ATTGTGATCC TTATATTCAT TATGACAGGG ATTGACCTGA AGTTCTGGCA TAAAAAAATG 
GATTTCTCCC AGAATGTAAA CGTCAGCAGT CTATCAGGAC ACAATTACTT GTGCCCAAAT 
GACTGGCTGT TGAACGAAGG GAAATGTTAC TGGTTTTCAA CTTCTTTTAA AACGTGGAAA 
GAGAGTCAAC GTGATTGTAC ACAGCTACAG GCACATTTAC TGGTGATTCA AAATTTGGAT 
GAGCTGGAGT TCATACAGAA CAGTTTAAAA CCTGGACATT TTGGTTGGAT TGGACTATAT 
GTTACATTCC AAGGGAACCT ATGGATGTGG ATAGATGAAC ACTTTTTAGT TCCAGAATTG 
TTTTCAGTGA TTGGACCAAC TGATGACAGG AGCTGTGCCG TTATCACAGG AAACTGGGTG 
TATTCTGAAG ACTGTAGCTC CACATTTAAG GGCATTTGCC AGAGAGATGC GATCTTGACG 
CACAATGGAA CCAGTGGTGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177694.1
CDS	108..731
Translation	MENEDGYMTLSFKNRCKSKQKSKDFSLYPQYYCLLLIFGCIVILIFIMTGIDLKFWHKKMDFSQNVNVSSLSGHNYLCPNDWLLNEGKCYWFSTSFKTWKESQRDCTQLQAHLLVIQNLDELEFIQNSLKPGHFGWIGLYVTFQGNLWMWIDEHFLVPELFSVIGPTDDRSCAVITGNWVYSEDCSSTFKGICQRDAILTHNGTSGV

Target ORF information:

RefSeq Version	NM_001190765.1
Organism	Homo sapiens(human)
Definition	Homo sapiens killer cell lectin like receptor F2 (KLRF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190765.1

ORF Insert Sequence:

ATGGAGAATG AAGATGGGTA TATGACGCTG AGTTTCAAGA ATCGTTGTAA ATCGAAGCAG 
AAATCTAAAG ATTTCTCCCT ATATCCACAA TATTATTGTC TTCTGCTCAT ATTTGGATGC 
ATTGTGATCC TTATATTCAT TATGACAGGG ATTGACCTGA AGTTCTGGCA TAAAAAAATG 
GATTTCTCCC AGAATGTAAA CGTCAGCAGT CTATCAGGAC ACAATTACTT GTGCCCAAAT 
GACTGGCTGT TGAACGAAGG GAAATGTTAC TGGTTTTCAA CTTCTTTTAA AACGTGGAAA 
GAGAGTCAAC GTGATTGTAC ACAGCTACAG GCACATTTAC TGGTGATTCA AAATTTGGAT 
GAGCTGGAGT TCATACAGAA CAGTTTAAAA CCTGGACATT TTGGTTGGAT TGGACTATAT 
GTTACATTCC AAGGGAACCT ATGGATGTGG ATAGATGAAC ACTTTTTAGT TCCAGAATTG 
TTTTCAGTGA TTGGACCAAC TGATGACAGG AGCTGTGCCG TTATCACAGG AAACTGGGTG 
TATTCTGAAG ACTGTAGCTC CACATTTAAG GGCATTTGCC AGAGAGATGC GATCTTGACG 
CACAATGGAA CCAGTGGTGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Activating C-type Lectin-like Receptor NKp65 Signals through a Hemi-immunoreceptor Tyrosine-based Activation Motif (hemITAM) and Spleen Tyrosine Kinase (Syk).
The Journal of biological chemistry292(8)3213-3223(2017 02)
Bauer B,Wotapek T,Z?ller T,Rutkowski E,Steinle A

Key residues at the membrane-distal surface of KACL, but not glycosylation, determine the functional interaction of the keratinocyte-specific C-type lectin-like receptor KACL with its high-affinity receptor NKp65.
Immunology145(1)114-23(2015 May)
Bauer B,Spreu J,Rohe C,Vogler I,Steinle A

Structure of NKp65 bound to its keratinocyte ligand reveals basis for genetically linked recognition in natural killer gene complex.
Proceedings of the National Academy of Sciences of the United States of America110(28)11505-10(2013 Jul)
Li Y,Wang Q,Chen S,Brown PH,Mariuzza RA

Interaction of C-type lectin-like receptors NKp65 and KACL facilitates dedicated immune recognition of human keratinocytes.
Proceedings of the National Academy of Sciences of the United States of America107(11)5100-5(2010 Mar)
Spreu J,Kuttruff S,Stejfova V,Dennehy KM,Schittek B,Steinle A

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KLRF2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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