pnkp cDNA ORF clone, Hippocampus comes(tiger tail seahorse)

Gene
Clones
gRNAs

Summary

Gene Symbol	pnkp
Entrez Gene ID	109529349
Full Name	polynucleotide kinase 3'-phosphatase
Gene Type	protein-coding
Organism	Hippocampus comes(tiger tail seahorse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019892681.1	XP_019748240.1	bifunctional polynucleotide phosphatase/kinase

Pathways from BioSystems
Gene Ontology

The following pnkp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pnkp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHi62401	XM_019892681.1 Latest version!	Hippocampus comes polynucleotide kinase 3'-phosphatase (pnkp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pnkp ( XM_019892681.1 ) cDNA ORF clone, Hippocampus comes(tiger tail seahorse) -> XP_019748240.1 Hippocampus comes polynucleotide kinase 3'-phosphatase (pnkp), mRNA.

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CloneID	OHi62401
Clone ID Related Accession (Same CDS sequence)	XM_019892681.1
Accession Version	XM_019892681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Hippocampus comes(tiger tail seahorse)
Product	bifunctional polynucleotide phosphatase/kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017806030.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Hippocampus comes Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTGCG TTTTGGTTAG TTCATCTGGG GCTCAAATCC TTCTGGAGGA TGGACGAGCC 
GTGTTGTTGG GTCGTGGACC CGATACCGGA GTCGTCGACA AAAAGTGCTC AAGACACCAA 
GTGAAGGTGG TGGCTTGCTT TGCAGACCAG GATGTGGTTG TCACTCAGTT GGGTCCCAAT 
CCCAGTTTCC TGGAGAGTGA GAAGCTTAGC CGTGGCCATT CTGGTAAACT CAGTCATGGT 
GACACCCTCT ACCTGGTCAA CCATGAATAC CCATTCAAAG TGTGCTTCAA CTCGAGCTCT 
GCTGGACCTT CTTTCATTAC GGTACAAGGA AAGCTCAACA ATTCGAACAA AACAAAAAGT 
GGTAAGAATG GGAATGAACA AAGTTCACCG TGGAGTCCAC CTAAGCGCAG TATAAAGGAT 
TTCTTTCCTA CCTCTCCCAT GAAGTCATCA AAAAGGCCGC TTAGCCCAGA GAAAGGGCAT 
CCTGATGCGA AGCGCCAAAG AGGAAATGAG GCCTCGGGTG AATGGGATAG AGAGGAGGTG 
AGCGAAGATG AGAATGAGAA ACGAGCAGAA GAAAAGCTCA GGCAGCTCCA GGAGTTGGCA 
GAGTCAGCCA TGAATGAGAA ACGGAACAGG AGCCCAGCAG AAACCGGAGC CCAACCTACA 
ATTTCTTCTT CCTCGGCCTG TGTAAAGAGC ACTTGGCAGC AAATTGGCAA CCTCATTCTT 
TACACTGCAG CAGGTGTCAG AGGGAGTGAC AAGATTGCTG GGTTTGACAT TGATGGCTGC 
ATTATCACCA CTAAATCAGG CAAAGTCTTT CCCACAGCAC CGGATGACTG GAAGATACTC 
TACCCTGAAA TTCCGCCTCG GCTGGCCAGC TTAACCAAGA AAGGATACAA GGTGGTGTTT 
TTCACCAATC AGATGGGGAT CGCCAGAGGA AAACTGAGAC CAGAAGTCTT CAAGTCGAAA 
GTGGAGGACA TCCTCGCCAC ACTGGAGCTG CCTGTGCAGG TGTTTGTTGC AGCTGGTCCA 
GGAATCTACA GGAAGCCAGC AGTGGGAATG TGGAATCACC TGTGTGAAAA GGCAAATGAT 
GGCGTTCCTG TGGACAAGAC ACAAAGTTTT TATGTTGGAG ATGCTGCAGG TAGACCTGAG 
AACTGGGCTC CAGGGAAGAA GAAGAAGGAC TTCTCCTGCA GTGACCGACT GTTTTCCCTG 
AATCTTAGTC TGCAGTTTCA CACTCCAGAG GAGTACTTCC TGGGCTGGAA GAGCGCCCCT 
TATAGTCTAC CACAATTTGA CCCTAGAAAA CTGGACTCCA GCATCAGGCT ATATGACCCG 
CCGTCTGCGT CTCTCACCTC GAGCACGACA GAAGTCATTG TGGCTGTGGG TTTCCCTGCA 
TCGGGCAAGT CCACATTCTT CCACACCCAT ATCATCCCAA AGGGTTATGC GTACATCAAC 
AGGGACACGC TTGGTTCGTG GCAGAACTGT GTGGCAGCAT GTAAGCGTGC TCTGCAGGAG 
GGCCGCAGCG TCGCCGTAGA CAACACCAAC CCAGACCCCG AGTCTCGCAA ACGTTACGTG 
GATGTGGCCA AGGCCGCAGG TGTATCCTGT CGCTGTTTTC ACTTCTCAGC CACTCTGGAA 
CAAGCCAAAC ACAACAATAG ATTCCGCGAG ATGGCGCCCT CAGACAGCAA ACATGACAAA 
GTCAGTGACA TTGTTTTCCA AAGCTATAAG AAACACTTCG TGGCACCTGC TCTCTCTGAG 
GGATTCTCCG AGATCCTCAG CATCCACTTT GTCCCTGACT TTAAAGACAG CCAATCAGAA 
ATCTTATTCC GACAATTCTC TGAAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019748240.1
CDS	212..2041
Translation	MSCVLVSSSGAQILLEDGRAVLLGRGPDTGVVDKKCSRHQVKVVACFADQDVVVTQLGPNPSFLESEKLSRGHSGKLSHGDTLYLVNHEYPFKVCFNSSSAGPSFITVQGKLNNSNKTKSGKNGNEQSSPWSPPKRSIKDFFPTSPMKSSKRPLSPEKGHPDAKRQRGNEASGEWDREEVSEDENEKRAEEKLRQLQELAESAMNEKRNRSPAETGAQPTISSSSACVKSTWQQIGNLILYTAAGVRGSDKIAGFDIDGCIITTKSGKVFPTAPDDWKILYPEIPPRLASLTKKGYKVVFFTNQMGIARGKLRPEVFKSKVEDILATLELPVQVFVAAGPGIYRKPAVGMWNHLCEKANDGVPVDKTQSFYVGDAAGRPENWAPGKKKKDFSCSDRLFSLNLSLQFHTPEEYFLGWKSAPYSLPQFDPRKLDSSIRLYDPPSASLTSSTTEVIVAVGFPASGKSTFFHTHIIPKGYAYINRDTLGSWQNCVAACKRALQEGRSVAVDNTNPDPESRKRYVDVAKAAGVSCRCFHFSATLEQAKHNNRFREMAPSDSKHDKVSDIVFQSYKKHFVAPALSEGFSEILSIHFVPDFKDSQSEILFRQFSEG

Target ORF information:

RefSeq Version	XM_019892681.1
Organism	Hippocampus comes(tiger tail seahorse)
Definition	Hippocampus comes polynucleotide kinase 3'-phosphatase (pnkp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019892681.1

ORF Insert Sequence:

ATGTCGTGCG TTTTGGTTAG TTCATCTGGG GCTCAAATCC TTCTGGAGGA TGGACGAGCC 
GTGTTGTTGG GTCGTGGACC CGATACCGGA GTCGTCGACA AAAAGTGCTC AAGACACCAA 
GTGAAGGTGG TGGCTTGCTT TGCAGACCAG GATGTGGTTG TCACTCAGTT GGGTCCCAAT 
CCCAGTTTCC TGGAGAGTGA GAAGCTTAGC CGTGGCCATT CTGGTAAACT CAGTCATGGT 
GACACCCTCT ACCTGGTCAA CCATGAATAC CCATTCAAAG TGTGCTTCAA CTCGAGCTCT 
GCTGGACCTT CTTTCATTAC GGTACAAGGA AAGCTCAACA ATTCGAACAA AACAAAAAGT 
GGTAAGAATG GGAATGAACA AAGTTCACCG TGGAGTCCAC CTAAGCGCAG TATAAAGGAT 
TTCTTTCCTA CCTCTCCCAT GAAGTCATCA AAAAGGCCGC TTAGCCCAGA GAAAGGGCAT 
CCTGATGCGA AGCGCCAAAG AGGAAATGAG GCCTCGGGTG AATGGGATAG AGAGGAGGTG 
AGCGAAGATG AGAATGAGAA ACGAGCAGAA GAAAAGCTCA GGCAGCTCCA GGAGTTGGCA 
GAGTCAGCCA TGAATGAGAA ACGGAACAGG AGCCCAGCAG AAACCGGAGC CCAACCTACA 
ATTTCTTCTT CCTCGGCCTG TGTAAAGAGC ACTTGGCAGC AAATTGGCAA CCTCATTCTT 
TACACTGCAG CAGGTGTCAG AGGGAGTGAC AAGATTGCTG GGTTTGACAT TGATGGCTGC 
ATTATCACCA CTAAATCAGG CAAAGTCTTT CCCACAGCAC CGGATGACTG GAAGATACTC 
TACCCTGAAA TTCCGCCTCG GCTGGCCAGC TTAACCAAGA AAGGATACAA GGTGGTGTTT 
TTCACCAATC AGATGGGGAT CGCCAGAGGA AAACTGAGAC CAGAAGTCTT CAAGTCGAAA 
GTGGAGGACA TCCTCGCCAC ACTGGAGCTG CCTGTGCAGG TGTTTGTTGC AGCTGGTCCA 
GGAATCTACA GGAAGCCAGC AGTGGGAATG TGGAATCACC TGTGTGAAAA GGCAAATGAT 
GGCGTTCCTG TGGACAAGAC ACAAAGTTTT TATGTTGGAG ATGCTGCAGG TAGACCTGAG 
AACTGGGCTC CAGGGAAGAA GAAGAAGGAC TTCTCCTGCA GTGACCGACT GTTTTCCCTG 
AATCTTAGTC TGCAGTTTCA CACTCCAGAG GAGTACTTCC TGGGCTGGAA GAGCGCCCCT 
TATAGTCTAC CACAATTTGA CCCTAGAAAA CTGGACTCCA GCATCAGGCT ATATGACCCG 
CCGTCTGCGT CTCTCACCTC GAGCACGACA GAAGTCATTG TGGCTGTGGG TTTCCCTGCA 
TCGGGCAAGT CCACATTCTT CCACACCCAT ATCATCCCAA AGGGTTATGC GTACATCAAC 
AGGGACACGC TTGGTTCGTG GCAGAACTGT GTGGCAGCAT GTAAGCGTGC TCTGCAGGAG 
GGCCGCAGCG TCGCCGTAGA CAACACCAAC CCAGACCCCG AGTCTCGCAA ACGTTACGTG 
GATGTGGCCA AGGCCGCAGG TGTATCCTGT CGCTGTTTTC ACTTCTCAGC CACTCTGGAA 
CAAGCCAAAC ACAACAATAG ATTCCGCGAG ATGGCGCCCT CAGACAGCAA ACATGACAAA 
GTCAGTGACA TTGTTTTCCA AAGCTATAAG AAACACTTCG TGGCACCTGC TCTCTCTGAG 
GGATTCTCCG AGATCCTCAG CATCCACTTT GTCCCTGACT TTAAAGACAG CCAATCAGAA 
ATCTTATTCC GACAATTCTC TGAAGGCTGA