nefm cDNA ORF clone, Hippocampus comes(tiger tail seahorse)

Gene
Clones
gRNAs

Summary

Gene Symbol	nefm
Entrez Gene ID	109508091
Full Name	neurofilament medium
Gene Type	protein-coding
Organism	Hippocampus comes(tiger tail seahorse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019857932.1	XP_019713491.1	neurofilament medium polypeptide isoform X1
XM_019857933.1	XP_019713492.1	neurofilament medium polypeptide isoform X2

Pathways from BioSystems
Gene Ontology

The following nefm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nefm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHi67897	XM_019857932.1 Latest version!	Hippocampus comes neurofilament medium (nefm), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OHi67898	XM_019857933.1 Latest version!	Hippocampus comes neurofilament medium (nefm), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nefm ( XM_019857932.1 ) cDNA ORF clone, Hippocampus comes(tiger tail seahorse) -> XP_019713491.1 Hippocampus comes neurofilament medium (nefm), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHi67897
Clone ID Related Accession (Same CDS sequence)	XM_019857932.1
Accession Version	XM_019857932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Hippocampus comes(tiger tail seahorse)
Product	neurofilament medium polypeptide isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017806279.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Hippocampus comes Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGATAC TCATCATATA TAAGGTGCAC AAGGAGAAAG TTGCAGTCGC TGTGGAAGCC 
ATCCACCCCT CACACCTGCA CCCACACGCA TACATACACT CCCCGTCGCC ACCCCCTATC 
ACAGCCACAT ACTGTAGAGC ACAGCCACAG AGCCAGTTTC CTCAAATTAG TTCCCGCACA 
CTTGGCCGGA TGGACGCCAT AGGGAGCCCC TTTCGGAGAG TTACGGACAG CAGGGCGAGC 
AGTTACAGCT ACAGCCGCTC CGTAGCCGGC CCCTCCGCCG GCGGATACCG CTCCCAGACG 
TGGTCTCGGA CCAGTCCGGC TTCAGGCGTG AGCGTGTCGT ACAAGAGGGG CGCCAACAAG 
CCCCGCTTGG ACACCTTCGA GCTGAGCCAA AGTTCCCTCC TGAGCGCGGA GTCCTCCCGG 
CGCTCCAACG AAAAGGAGCA ACTCCAAGGG CTCAACGACC GCTTCGCCGT GTACATCGAC 
AAGGTGCACT ACCTGGAGCA GCAAAACAAA CAGATCGAGG ACGAGATCCA GGCCCTGAGG 
CACCAGCAGG TGTCCCGCTC GCAGCTGGGT GACCTGTTCG ACCAGGAGCT GCAAGAGCTG 
CGCGCCACAC TGGAGCAGAT CCACCGCGAC AAGGCGCAGA TCCAGCTCGA CAGCGACCAC 
CTGGAGGACG ACATCCAGAG GCTTAGGGAG CGCCTGGACG AGGAAGCCCG CATCCGGGAG 
GACACCGAGG CCATCATCCG CGTTCTCAAG AAAGAGGCCG GAGACTCGGA CATGCTCAAA 
TCCGAGCTGG AAAAGAAAGT GCAATCGCTG CAGGACGAGA TCGCTTTCAT CCGCAACAAC 
CACGACGAGG AAGTGAACGA GCTTCTGGGC CACATCCAGG CGGCACAGGT GACCGTGGAG 
AGGAGGGACC CGCAGCGGGC CGACATCACG GAGGCTCTGC GCGAGATCCG CAGCGAGCTG 
GAGGGCCACT CGGACAAGAA CCTGGAGCAG GTGGAGAGCT GGTTCATGTG CCACTACGCC 
AAGTTGAACG AGGCGTCCGA ACAAAACAAA GACGCCATCA AGAGCGCTCG CGACGAGATC 
AGCGATTACC GGCGCCAGCT GCAGTCCAAG ACTGTGGAGT TGGAATCGGT GCGCGGCACC 
AGAGACTCCC TGGAGAGGCA GCTGGGCGAC ATCGAGGACC GCCACAACAG CGACCTGGCC 
AGCCTGCAGG AGACCATCCA CCAGCTGGAC AATGAGCTCA AGAGCACCAA ATGGGAGATG 
GCGCGTCATT TACGCGAGTA CCAGGATCTG CTCAATGTCA AGATGGCCCT GGACATTGAG 
ATCGCTGCAT ACAGGAAACT CCTGGAGGGC GAGGAGACCC ACTTCAGCAC CTTCGCCCAT 
CGGCCAGCCG TTTCATCCAC CAGAATCTCA ATTTCCAAAT CAGAACCTCC CAGGATGAAG 
GTGCAACACA AGTTTGTGGA GGAGATCATC GAGGAAACCA GGGTGGAGGA TGACAAGGCC 
GACATGGAGG ACGCCCTGGA TCAAATAACC CAGAAGCTCT CGGCCACACT CGAAGGCAGC 
GGAGGCGAGG AGGACGAAGA GGAAGGTGAA GAGGATGGCG AGGTGGAGGA AGAGGAAGTC 
GTAACTGCAA CTGCTGCGAA AGTGAGTTTG AAGGCGCCGG CGAAGGAGGA AGAGGGGGAT 
GATGAAGAGG ATGGGGAGGA GGAAGAAGAG GCAGGGGAAG GTGATGAGGA GCAAAATGAA 
GAAGATGAGG ATGAGGACAA TAAAGAAGAG GAGGAGGCGG GCGATGAGGA GGCGGTAGTA 
GAGGAAACTG TCCTGTGCTC CAAATCTCAA GAGTCCAACA CCATTCCTGG GAAAGTGAAG 
GCCGGAGAGG AGGAAGAGGC CAGTGAAGAG GAAGCTGATG CCACTGAGGA TGAAGACAAA 
GCATCCAAAG ATGGAGACGA CGAAGAAGAA AAGGCAGATG CAGACCAGGG GAAGAAAGAG 
GATGAGAAGG TTGCTGTTGA AGACATTGTG ACAGAAACAA TTGTAGCCAA AGTACACGTT 
CAGAAAACAG AAGCTGCAAA ACCTGAAGAA CTGACAAAAC CAGATTCTCT TAAACCAGAA 
TCTCCAAAAG GTGGATCCCC AAAGTCTGAA TCCCCAAAAG CTGAATCATT AAAAGCTGCT 
TCCCCCAAAT CTGAATCCCC AAAAGCTGGC TCCCCAAAAT CTGAATCCCC AAAAGCTGCT 
TCCCCAAAAT CTGAATCCCC AAAAGCTGGC TCCCCCAAAA CAGAATCCCC CAAAGCTACT 
TCCCCCAAGC CAGAATCACC TAAGGCAGAG TCCCCTAAAT CAGAATCTCC CAAGCCTGGA 
TCCCCCAAAG AAACCTCTCC GAAAACTACT CCCCCAAAAG CAGAGTCCCC AAAACCGGAA 
GCCACCAAGC CTGCATCCCC GACAAAAGAC TCACCGAAGG CTGGCTCCCC AAAAGAGGAG 
TCCCCTAAAT CTGAATTCCC CAAATCTGTT TCCCCCAAAG AAGGCTCACC GAAGGCTGGC 
TCCCCAAAAG AGGAGTCCCC TAAATCTGAA TCTCCCAAGC CCGGATCCCC CAAGGAAGAC 
TCACCAAAGG CTGGCTCCCC AAACGCAGAG TCCCCTAAGT CTGAATCAGC AAAACCGGAC 
TCGCCAAAGA CTGAATCGAC TAAACCTGAT ACCTCCAAAC CTGCCGAAGA CATAAAGAGT 
GACACCACAG ACGACAAGAA GACGAAAGAC GCCGCCATGA ATGGTGACCT GGACAAGAGC 
AGCCCAAAGG AAGAGGAGAA AAAGCGGGGC GAGGAGACCG ACGTGATCAT CAACGGTGTG 
GACGAGAGCC CCGTCAAGGA CGACGGCAAC CAGAAGGTGG TGATCACCAA AACGGTAGAG 
ACCATCACCA CGGGGGGCGA CGGCTTGCAG CAGGTCACTA AGTCCGTCAC GGTTACCGAG 
ACGGTGAAGG AAGTTGAGGA CGTGCTGCAG GAGAAGTTCG TCTCCACCAT CAAGACGGAG 
AAGCACTCCA GCCAGTCCAT CAAGCAGGTG ACGGAAGGCG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019713491.1
CDS	27..3071
Translation	MPILIIYKVHKEKVAVAVEAIHPSHLHPHAYIHSPSPPPITATYCRAQPQSQFPQISSRTLGRMDAIGSPFRRVTDSRASSYSYSRSVAGPSAGGYRSQTWSRTSPASGVSVSYKRGANKPRLDTFELSQSSLLSAESSRRSNEKEQLQGLNDRFAVYIDKVHYLEQQNKQIEDEIQALRHQQVSRSQLGDLFDQELQELRATLEQIHRDKAQIQLDSDHLEDDIQRLRERLDEEARIREDTEAIIRVLKKEAGDSDMLKSELEKKVQSLQDEIAFIRNNHDEEVNELLGHIQAAQVTVERRDPQRADITEALREIRSELEGHSDKNLEQVESWFMCHYAKLNEASEQNKDAIKSARDEISDYRRQLQSKTVELESVRGTRDSLERQLGDIEDRHNSDLASLQETIHQLDNELKSTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETHFSTFAHRPAVSSTRISISKSEPPRMKVQHKFVEEIIEETRVEDDKADMEDALDQITQKLSATLEGSGGEEDEEEGEEDGEVEEEEVVTATAAKVSLKAPAKEEEGDDEEDGEEEEEAGEGDEEQNEEDEDEDNKEEEEAGDEEAVVEETVLCSKSQESNTIPGKVKAGEEEEASEEEADATEDEDKASKDGDDEEEKADADQGKKEDEKVAVEDIVTETIVAKVHVQKTEAAKPEELTKPDSLKPESPKGGSPKSESPKAESLKAASPKSESPKAGSPKSESPKAASPKSESPKAGSPKTESPKATSPKPESPKAESPKSESPKPGSPKETSPKTTPPKAESPKPEATKPASPTKDSPKAGSPKEESPKSEFPKSVSPKEGSPKAGSPKEESPKSESPKPGSPKEDSPKAGSPNAESPKSESAKPDSPKTESTKPDTSKPAEDIKSDTTDDKKTKDAAMNGDLDKSSPKEEEKKRGEETDVIINGVDESPVKDDGNQKVVITKTVETITTGGDGLQQVTKSVTVTETVKEVEDVLQEKFVSTIKTEKHSSQSIKQVTEGD

Target ORF information:

RefSeq Version	XM_019857932.1
Organism	Hippocampus comes(tiger tail seahorse)
Definition	Hippocampus comes neurofilament medium (nefm), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019857932.1

ORF Insert Sequence:

ATGCCGATAC TCATCATATA TAAGGTGCAC AAGGAGAAAG TTGCAGTCGC TGTGGAAGCC 
ATCCACCCCT CACACCTGCA CCCACACGCA TACATACACT CCCCGTCGCC ACCCCCTATC 
ACAGCCACAT ACTGTAGAGC ACAGCCACAG AGCCAGTTTC CTCAAATTAG TTCCCGCACA 
CTTGGCCGGA TGGACGCCAT AGGGAGCCCC TTTCGGAGAG TTACGGACAG CAGGGCGAGC 
AGTTACAGCT ACAGCCGCTC CGTAGCCGGC CCCTCCGCCG GCGGATACCG CTCCCAGACG 
TGGTCTCGGA CCAGTCCGGC TTCAGGCGTG AGCGTGTCGT ACAAGAGGGG CGCCAACAAG 
CCCCGCTTGG ACACCTTCGA GCTGAGCCAA AGTTCCCTCC TGAGCGCGGA GTCCTCCCGG 
CGCTCCAACG AAAAGGAGCA ACTCCAAGGG CTCAACGACC GCTTCGCCGT GTACATCGAC 
AAGGTGCACT ACCTGGAGCA GCAAAACAAA CAGATCGAGG ACGAGATCCA GGCCCTGAGG 
CACCAGCAGG TGTCCCGCTC GCAGCTGGGT GACCTGTTCG ACCAGGAGCT GCAAGAGCTG 
CGCGCCACAC TGGAGCAGAT CCACCGCGAC AAGGCGCAGA TCCAGCTCGA CAGCGACCAC 
CTGGAGGACG ACATCCAGAG GCTTAGGGAG CGCCTGGACG AGGAAGCCCG CATCCGGGAG 
GACACCGAGG CCATCATCCG CGTTCTCAAG AAAGAGGCCG GAGACTCGGA CATGCTCAAA 
TCCGAGCTGG AAAAGAAAGT GCAATCGCTG CAGGACGAGA TCGCTTTCAT CCGCAACAAC 
CACGACGAGG AAGTGAACGA GCTTCTGGGC CACATCCAGG CGGCACAGGT GACCGTGGAG 
AGGAGGGACC CGCAGCGGGC CGACATCACG GAGGCTCTGC GCGAGATCCG CAGCGAGCTG 
GAGGGCCACT CGGACAAGAA CCTGGAGCAG GTGGAGAGCT GGTTCATGTG CCACTACGCC 
AAGTTGAACG AGGCGTCCGA ACAAAACAAA GACGCCATCA AGAGCGCTCG CGACGAGATC 
AGCGATTACC GGCGCCAGCT GCAGTCCAAG ACTGTGGAGT TGGAATCGGT GCGCGGCACC 
AGAGACTCCC TGGAGAGGCA GCTGGGCGAC ATCGAGGACC GCCACAACAG CGACCTGGCC 
AGCCTGCAGG AGACCATCCA CCAGCTGGAC AATGAGCTCA AGAGCACCAA ATGGGAGATG 
GCGCGTCATT TACGCGAGTA CCAGGATCTG CTCAATGTCA AGATGGCCCT GGACATTGAG 
ATCGCTGCAT ACAGGAAACT CCTGGAGGGC GAGGAGACCC ACTTCAGCAC CTTCGCCCAT 
CGGCCAGCCG TTTCATCCAC CAGAATCTCA ATTTCCAAAT CAGAACCTCC CAGGATGAAG 
GTGCAACACA AGTTTGTGGA GGAGATCATC GAGGAAACCA GGGTGGAGGA TGACAAGGCC 
GACATGGAGG ACGCCCTGGA TCAAATAACC CAGAAGCTCT CGGCCACACT CGAAGGCAGC 
GGAGGCGAGG AGGACGAAGA GGAAGGTGAA GAGGATGGCG AGGTGGAGGA AGAGGAAGTC 
GTAACTGCAA CTGCTGCGAA AGTGAGTTTG AAGGCGCCGG CGAAGGAGGA AGAGGGGGAT 
GATGAAGAGG ATGGGGAGGA GGAAGAAGAG GCAGGGGAAG GTGATGAGGA GCAAAATGAA 
GAAGATGAGG ATGAGGACAA TAAAGAAGAG GAGGAGGCGG GCGATGAGGA GGCGGTAGTA 
GAGGAAACTG TCCTGTGCTC CAAATCTCAA GAGTCCAACA CCATTCCTGG GAAAGTGAAG 
GCCGGAGAGG AGGAAGAGGC CAGTGAAGAG GAAGCTGATG CCACTGAGGA TGAAGACAAA 
GCATCCAAAG ATGGAGACGA CGAAGAAGAA AAGGCAGATG CAGACCAGGG GAAGAAAGAG 
GATGAGAAGG TTGCTGTTGA AGACATTGTG ACAGAAACAA TTGTAGCCAA AGTACACGTT 
CAGAAAACAG AAGCTGCAAA ACCTGAAGAA CTGACAAAAC CAGATTCTCT TAAACCAGAA 
TCTCCAAAAG GTGGATCCCC AAAGTCTGAA TCCCCAAAAG CTGAATCATT AAAAGCTGCT 
TCCCCCAAAT CTGAATCCCC AAAAGCTGGC TCCCCAAAAT CTGAATCCCC AAAAGCTGCT 
TCCCCAAAAT CTGAATCCCC AAAAGCTGGC TCCCCCAAAA CAGAATCCCC CAAAGCTACT 
TCCCCCAAGC CAGAATCACC TAAGGCAGAG TCCCCTAAAT CAGAATCTCC CAAGCCTGGA 
TCCCCCAAAG AAACCTCTCC GAAAACTACT CCCCCAAAAG CAGAGTCCCC AAAACCGGAA 
GCCACCAAGC CTGCATCCCC GACAAAAGAC TCACCGAAGG CTGGCTCCCC AAAAGAGGAG 
TCCCCTAAAT CTGAATTCCC CAAATCTGTT TCCCCCAAAG AAGGCTCACC GAAGGCTGGC 
TCCCCAAAAG AGGAGTCCCC TAAATCTGAA TCTCCCAAGC CCGGATCCCC CAAGGAAGAC 
TCACCAAAGG CTGGCTCCCC AAACGCAGAG TCCCCTAAGT CTGAATCAGC AAAACCGGAC 
TCGCCAAAGA CTGAATCGAC TAAACCTGAT ACCTCCAAAC CTGCCGAAGA CATAAAGAGT 
GACACCACAG ACGACAAGAA GACGAAAGAC GCCGCCATGA ATGGTGACCT GGACAAGAGC 
AGCCCAAAGG AAGAGGAGAA AAAGCGGGGC GAGGAGACCG ACGTGATCAT CAACGGTGTG 
GACGAGAGCC CCGTCAAGGA CGACGGCAAC CAGAAGGTGG TGATCACCAA AACGGTAGAG 
ACCATCACCA CGGGGGGCGA CGGCTTGCAG CAGGTCACTA AGTCCGTCAC GGTTACCGAG 
ACGGTGAAGG AAGTTGAGGA CGTGCTGCAG GAGAAGTTCG TCTCCACCAT CAAGACGGAG 
AAGCACTCCA GCCAGTCCAT CAAGCAGGTG ACGGAAGGCG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nefm ( XM_019857933.1 ) cDNA ORF clone, Hippocampus comes(tiger tail seahorse) -> XP_019713492.1 Hippocampus comes neurofilament medium (nefm), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHi67898
Clone ID Related Accession (Same CDS sequence)	XM_019857933.1
Accession Version	XM_019857933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Hippocampus comes(tiger tail seahorse)
Product	neurofilament medium polypeptide isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017806279.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Hippocampus comes Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGATAC TCATCATATA TAAGGTGCAC AAGGAGAAAG TTGCAGTCGC TGTGGAAGCC 
ATCCACCCCT CACACCTGCA CCCACACGCA TACATACACT CCCCGTCGCC ACCCCCTATC 
ACAGCCACAT ACTGTAGAGC ACAGCCACAG AGCCAGTTTC CTCAAATTAG TTCCCGCACA 
CTTGGCCGGA TGGACGCCAT AGGGAGCCCC TTTCGGAGAG TTACGGACAG CAGGGCGAGC 
AGTTACAGCT ACAGCCGCTC CGTAGCCGGC CCCTCCGCCG GCGGATACCG CTCCCAGACG 
TGGTCTCGGA CCAGTCCGGC TTCAGGCGTG AGCGTGTCGT ACAAGAGGGG CGCCAACAAG 
CCCCGCTTGG ACACCTTCGA GCTGAGCCAA AGTTCCCTCC TGAGCGCGGA GTCCTCCCGG 
CGCTCCAACG AAAAGGAGCA ACTCCAAGGG CTCAACGACC GCTTCGCCGT GTACATCGAC 
AAGGTGCACT ACCTGGAGCA GCAAAACAAA CAGATCGAGG ACGAGATCCA GGCCCTGAGG 
CACCAGCAGG TGTCCCGCTC GCAGCTGGGT GACCTGTTCG ACCAGGAGCT GCAAGAGCTG 
CGCGCCACAC TGGAGCAGAT CCACCGCGAC AAGGCGCAGA TCCAGCTCGA CAGCGACCAC 
CTGGAGGACG ACATCCAGAG GCTTAGGGAG CGCCTGGACG AGGAAGCCCG CATCCGGGAG 
GACACCGAGG CCATCATCCG CGTTCTCAAG AAAGAGGCCG GAGACTCGGA CATGCTCAAA 
TCCGAGCTGG AAAAGAAAGT GCAATCGCTG CAGGACGAGA TCGCTTTCAT CCGCAACAAC 
CACGACGAGG AAGTGAACGA GCTTCTGGGC CACATCCAGG CGGCACAGGT GACCGTGGAG 
AGGAGGGACC CGCAGCGGGC CGACATCACG GAGGCTCTGC GCGAGATCCG CAGCGAGCTG 
GAGGGCCACT CGGACAAGAA CCTGGAGCAG GTGGAGAGCT GGTTCATGTG CCACTACGCC 
AAGTTGAACG AGGCGTCCGA ACAAAACAAA GACGCCATCA AGAGCGCTCG CGACGAGATC 
AGCGATTACC GGCGCCAGCT GCAGTCCAAG ACTGTGGAGT TGGAATCGGT GCGCGGCACC 
AGAGACTCCC TGGAGAGGCA GCTGGGCGAC ATCGAGGACC GCCACAACAG CGACCTGGCC 
AGCCTGCAGG AGACCATCCA CCAGCTGGAC AATGAGCTCA AGAGCACCAA ATGGGAGATG 
GCGCGTCATT TACGCGAGTA CCAGGATCTG CTCAATGTCA AGATGGCCCT GGACATTGAG 
ATCGCTGCAT ACAGGAAACT CCTGGAGGGC GAGGAGACCC ACTTCAGCAC CTTCGCCCAT 
CGGCCAGCCG TTTCATCCAC CAGAATCTCA ATTTCCAAAT CAGAACCTCC CAGGATGAAG 
GTGCAACACA AGTTTGTGGA GGAGATCATC GAGGAAACCA GGGTGGAGGA TGACAAGGCC 
GACATGGAGG ACGCCCTGGA TCAAATAACC CAGAAGCTCT CGGCCACACT CGAAGGCAGC 
GGAGGCGAGG AGGACGAAGA GGAAGGTGAA GAGGATGGCG AGGTGGAGGA AGAGGAAGTC 
GTAACTGCAA CTGCTGCGAA AGTGAGTTTG AAGGCGCCGG CGAAGGAGGA AGAGGGGGAT 
GATGAAGAGG ATGGGGAGGA GGAAGAAGAG GCAGGGGAAG GTGATGAGGA GCAAAATGAA 
GAAGATGAGG ATGAGGACAA TAAAGAAGAG GAGGAGGCGG GCGATGAGGA GGCGGTAGTA 
GAGGAAACTG TCCTGTGCTC CAAATCTCAA GAGTCCAACA CCATTCCTGG GAAAGTGAAG 
GCCGGAGAGG AGGAAGAGGC CAGTGAAGAG GAAGCTGATG CCACTGAGGA TGAAGACAAA 
GCATCCAAAG ATGGAGACGA CGAAGAAGAA AAGGCAGATG CAGACCAGGG GAAGAAAGAG 
GATGAGAAGG TTGCTGTTGA AGACATTGTG ACAGAAACAA TTGTAGCCAA AGTACACGTT 
CAGAAAACAG AAGCTGCAAA ACCTGAAGAA CTGACAAAAC CAGATTCTCT TAAACCAGAA 
TCTCCAAAAG GTGGATCCCC AAAGTCTGAA TCCCCAAAAG CTGGCTCCCC AAAATCTGAA 
TCCCCAAAAG CTGCTTCCCC AAAATCTGAA TCCCCAAAAG CTGGCTCCCC CAAAACAGAA 
TCCCCCAAAG CTACTTCCCC CAAGCCAGAA TCACCTAAGG CAGAGTCCCC TAAATCAGAA 
TCTCCCAAGC CTGGATCCCC CAAAGAAACC TCTCCGAAAA CTACTCCCCC AAAAGCAGAG 
TCCCCAAAAC CGGAAGCCAC CAAGCCTGCA TCCCCGACAA AAGACTCACC GAAGGCTGGC 
TCCCCAAAAG AGGAGTCCCC TAAATCTGAA TTCCCCAAAT CTGTTTCCCC CAAAGAAGGC 
TCACCGAAGG CTGGCTCCCC AAAAGAGGAG TCCCCTAAAT CTGAATCTCC CAAGCCCGGA 
TCCCCCAAGG AAGACTCACC AAAGGCTGGC TCCCCAAACG CAGAGTCCCC TAAGTCTGAA 
TCAGCAAAAC CGGACTCGCC AAAGACTGAA TCGACTAAAC CTGATACCTC CAAACCTGCC 
GAAGACATAA AGAGTGACAC CACAGACGAC AAGAAGACGA AAGACGCCGC CATGAATGGT 
GACCTGGACA AGAGCAGCCC AAAGGAAGAG GAGAAAAAGC GGGGCGAGGA GACCGACGTG 
ATCATCAACG GTGTGGACGA GAGCCCCGTC AAGGACGACG GCAACCAGAA GGTGGTGATC 
ACCAAAACGG TAGAGACCAT CACCACGGGG GGCGACGGCT TGCAGCAGGT CACTAAGTCC 
GTCACGGTTA CCGAGACGGT GAAGGAAGTT GAGGACGTGC TGCAGGAGAA GTTCGTCTCC 
ACCATCAAGA CGGAGAAGCA CTCCAGCCAG TCCATCAAGC AGGTGACGGA AGGCGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019713492.1
CDS	30..3029
Translation	MPILIIYKVHKEKVAVAVEAIHPSHLHPHAYIHSPSPPPITATYCRAQPQSQFPQISSRTLGRMDAIGSPFRRVTDSRASSYSYSRSVAGPSAGGYRSQTWSRTSPASGVSVSYKRGANKPRLDTFELSQSSLLSAESSRRSNEKEQLQGLNDRFAVYIDKVHYLEQQNKQIEDEIQALRHQQVSRSQLGDLFDQELQELRATLEQIHRDKAQIQLDSDHLEDDIQRLRERLDEEARIREDTEAIIRVLKKEAGDSDMLKSELEKKVQSLQDEIAFIRNNHDEEVNELLGHIQAAQVTVERRDPQRADITEALREIRSELEGHSDKNLEQVESWFMCHYAKLNEASEQNKDAIKSARDEISDYRRQLQSKTVELESVRGTRDSLERQLGDIEDRHNSDLASLQETIHQLDNELKSTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETHFSTFAHRPAVSSTRISISKSEPPRMKVQHKFVEEIIEETRVEDDKADMEDALDQITQKLSATLEGSGGEEDEEEGEEDGEVEEEEVVTATAAKVSLKAPAKEEEGDDEEDGEEEEEAGEGDEEQNEEDEDEDNKEEEEAGDEEAVVEETVLCSKSQESNTIPGKVKAGEEEEASEEEADATEDEDKASKDGDDEEEKADADQGKKEDEKVAVEDIVTETIVAKVHVQKTEAAKPEELTKPDSLKPESPKGGSPKSESPKAGSPKSESPKAASPKSESPKAGSPKTESPKATSPKPESPKAESPKSESPKPGSPKETSPKTTPPKAESPKPEATKPASPTKDSPKAGSPKEESPKSEFPKSVSPKEGSPKAGSPKEESPKSESPKPGSPKEDSPKAGSPNAESPKSESAKPDSPKTESTKPDTSKPAEDIKSDTTDDKKTKDAAMNGDLDKSSPKEEEKKRGEETDVIINGVDESPVKDDGNQKVVITKTVETITTGGDGLQQVTKSVTVTETVKEVEDVLQEKFVSTIKTEKHSSQSIKQVTEGD

Target ORF information:

RefSeq Version	XM_019857933.1
Organism	Hippocampus comes(tiger tail seahorse)
Definition	Hippocampus comes neurofilament medium (nefm), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019857933.1

ORF Insert Sequence:

ATGCCGATAC TCATCATATA TAAGGTGCAC AAGGAGAAAG TTGCAGTCGC TGTGGAAGCC 
ATCCACCCCT CACACCTGCA CCCACACGCA TACATACACT CCCCGTCGCC ACCCCCTATC 
ACAGCCACAT ACTGTAGAGC ACAGCCACAG AGCCAGTTTC CTCAAATTAG TTCCCGCACA 
CTTGGCCGGA TGGACGCCAT AGGGAGCCCC TTTCGGAGAG TTACGGACAG CAGGGCGAGC 
AGTTACAGCT ACAGCCGCTC CGTAGCCGGC CCCTCCGCCG GCGGATACCG CTCCCAGACG 
TGGTCTCGGA CCAGTCCGGC TTCAGGCGTG AGCGTGTCGT ACAAGAGGGG CGCCAACAAG 
CCCCGCTTGG ACACCTTCGA GCTGAGCCAA AGTTCCCTCC TGAGCGCGGA GTCCTCCCGG 
CGCTCCAACG AAAAGGAGCA ACTCCAAGGG CTCAACGACC GCTTCGCCGT GTACATCGAC 
AAGGTGCACT ACCTGGAGCA GCAAAACAAA CAGATCGAGG ACGAGATCCA GGCCCTGAGG 
CACCAGCAGG TGTCCCGCTC GCAGCTGGGT GACCTGTTCG ACCAGGAGCT GCAAGAGCTG 
CGCGCCACAC TGGAGCAGAT CCACCGCGAC AAGGCGCAGA TCCAGCTCGA CAGCGACCAC 
CTGGAGGACG ACATCCAGAG GCTTAGGGAG CGCCTGGACG AGGAAGCCCG CATCCGGGAG 
GACACCGAGG CCATCATCCG CGTTCTCAAG AAAGAGGCCG GAGACTCGGA CATGCTCAAA 
TCCGAGCTGG AAAAGAAAGT GCAATCGCTG CAGGACGAGA TCGCTTTCAT CCGCAACAAC 
CACGACGAGG AAGTGAACGA GCTTCTGGGC CACATCCAGG CGGCACAGGT GACCGTGGAG 
AGGAGGGACC CGCAGCGGGC CGACATCACG GAGGCTCTGC GCGAGATCCG CAGCGAGCTG 
GAGGGCCACT CGGACAAGAA CCTGGAGCAG GTGGAGAGCT GGTTCATGTG CCACTACGCC 
AAGTTGAACG AGGCGTCCGA ACAAAACAAA GACGCCATCA AGAGCGCTCG CGACGAGATC 
AGCGATTACC GGCGCCAGCT GCAGTCCAAG ACTGTGGAGT TGGAATCGGT GCGCGGCACC 
AGAGACTCCC TGGAGAGGCA GCTGGGCGAC ATCGAGGACC GCCACAACAG CGACCTGGCC 
AGCCTGCAGG AGACCATCCA CCAGCTGGAC AATGAGCTCA AGAGCACCAA ATGGGAGATG 
GCGCGTCATT TACGCGAGTA CCAGGATCTG CTCAATGTCA AGATGGCCCT GGACATTGAG 
ATCGCTGCAT ACAGGAAACT CCTGGAGGGC GAGGAGACCC ACTTCAGCAC CTTCGCCCAT 
CGGCCAGCCG TTTCATCCAC CAGAATCTCA ATTTCCAAAT CAGAACCTCC CAGGATGAAG 
GTGCAACACA AGTTTGTGGA GGAGATCATC GAGGAAACCA GGGTGGAGGA TGACAAGGCC 
GACATGGAGG ACGCCCTGGA TCAAATAACC CAGAAGCTCT CGGCCACACT CGAAGGCAGC 
GGAGGCGAGG AGGACGAAGA GGAAGGTGAA GAGGATGGCG AGGTGGAGGA AGAGGAAGTC 
GTAACTGCAA CTGCTGCGAA AGTGAGTTTG AAGGCGCCGG CGAAGGAGGA AGAGGGGGAT 
GATGAAGAGG ATGGGGAGGA GGAAGAAGAG GCAGGGGAAG GTGATGAGGA GCAAAATGAA 
GAAGATGAGG ATGAGGACAA TAAAGAAGAG GAGGAGGCGG GCGATGAGGA GGCGGTAGTA 
GAGGAAACTG TCCTGTGCTC CAAATCTCAA GAGTCCAACA CCATTCCTGG GAAAGTGAAG 
GCCGGAGAGG AGGAAGAGGC CAGTGAAGAG GAAGCTGATG CCACTGAGGA TGAAGACAAA 
GCATCCAAAG ATGGAGACGA CGAAGAAGAA AAGGCAGATG CAGACCAGGG GAAGAAAGAG 
GATGAGAAGG TTGCTGTTGA AGACATTGTG ACAGAAACAA TTGTAGCCAA AGTACACGTT 
CAGAAAACAG AAGCTGCAAA ACCTGAAGAA CTGACAAAAC CAGATTCTCT TAAACCAGAA 
TCTCCAAAAG GTGGATCCCC AAAGTCTGAA TCCCCAAAAG CTGGCTCCCC AAAATCTGAA 
TCCCCAAAAG CTGCTTCCCC AAAATCTGAA TCCCCAAAAG CTGGCTCCCC CAAAACAGAA 
TCCCCCAAAG CTACTTCCCC CAAGCCAGAA TCACCTAAGG CAGAGTCCCC TAAATCAGAA 
TCTCCCAAGC CTGGATCCCC CAAAGAAACC TCTCCGAAAA CTACTCCCCC AAAAGCAGAG 
TCCCCAAAAC CGGAAGCCAC CAAGCCTGCA TCCCCGACAA AAGACTCACC GAAGGCTGGC 
TCCCCAAAAG AGGAGTCCCC TAAATCTGAA TTCCCCAAAT CTGTTTCCCC CAAAGAAGGC 
TCACCGAAGG CTGGCTCCCC AAAAGAGGAG TCCCCTAAAT CTGAATCTCC CAAGCCCGGA 
TCCCCCAAGG AAGACTCACC AAAGGCTGGC TCCCCAAACG CAGAGTCCCC TAAGTCTGAA 
TCAGCAAAAC CGGACTCGCC AAAGACTGAA TCGACTAAAC CTGATACCTC CAAACCTGCC 
GAAGACATAA AGAGTGACAC CACAGACGAC AAGAAGACGA AAGACGCCGC CATGAATGGT 
GACCTGGACA AGAGCAGCCC AAAGGAAGAG GAGAAAAAGC GGGGCGAGGA GACCGACGTG 
ATCATCAACG GTGTGGACGA GAGCCCCGTC AAGGACGACG GCAACCAGAA GGTGGTGATC 
ACCAAAACGG TAGAGACCAT CACCACGGGG GGCGACGGCT TGCAGCAGGT CACTAAGTCC 
GTCACGGTTA CCGAGACGGT GAAGGAAGTT GAGGACGTGC TGCAGGAGAA GTTCGTCTCC 
ACCATCAAGA CGGAGAAGCA CTCCAGCCAG TCCATCAAGC AGGTGACGGA AGGCGATTGA