LOC110377785 cDNA ORF clone, Helicoverpa armigera(cotton bollworm)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110377785
Entrez Gene ID	110377785
Full Name	voltage-dependent calcium channel subunit alpha-2/delta-3
Gene Type	protein-coding
Organism	Helicoverpa armigera(cotton bollworm)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021336802.1	XP_021192477.1	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X1
XM_021336803.1	XP_021192478.1	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC110377785 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110377785 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHe209040	XM_021336802.1 Latest version!	Helicoverpa armigera voltage-dependent calcium channel subunit alpha-2/delta-3 (LOC110377785), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OHe209041	XM_021336803.1 Latest version!	Helicoverpa armigera voltage-dependent calcium channel subunit alpha-2/delta-3 (LOC110377785), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110377785 ( XM_021336802.1 ) cDNA ORF clone, Helicoverpa armigera(cotton bollworm) -> XP_021192477.1 Helicoverpa armigera voltage-dependent calcium channel subunit alpha-2/delta-3 (LOC110377785), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHe209040
Clone ID Related Accession (Same CDS sequence)	XM_021336802.1
Accession Version	XM_021336802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Helicoverpa armigera(cotton bollworm)
Product	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395552.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Helicoverpa armigera Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGA TGGCCGGCAT GTGTCCGCGG AGGCCGCTGG GCCTGCTGCT CGCGCTGCTC 
GCCGCCGCCG CGCTCGCCAA CCAGCTCGAA GACATCCCGC ACAATGAAGT CAAGAATTGG 
GCGCTAAAAT TCGGTGTCGA CTTATGGGAG TTTGGCCGGC ATTTCACAAA AATGAATCAA 
ATACAAAACA AATACCACGA GTACAACGTG GATGTGGTGC GCAAGGATGG GCTGCTTCTG 
GTGCGAGAGC TCGCAGCCGA AGTCAAGAAC CACATGGACT TCAAGATCAA CGCTGTCATG 
CGCATCATGG ACAGCGCTGA AGCGGCAGCT CTATCAGCCG GGAACGGCGG CAGCGATGGA 
GCACCAGGCT CGTACTATGA CGCTCGCTGG CTGAACGTGC ACGCTGATGA CGGGACCCTG 
GCTGCGAGAG CGCGACGCCT GGCGCTCACG CCGAGCCGAC ACTTCGACCA TATTGCCGTC 
AACACCAGCT ATAGTGCTGT GCTTATGCCG CCTTATATTA ACACTGATGA TGGCGAGGTA 
CAGAACCAGA TCGCGTGGTC GGAGCACTTG GACCCCCTGT TCGTGAACAA CTATGAGATC 
GACCCCACGC TTTCGTGGCA GTACTACGCC TCGTCTTCAG GATTCATGAG GCGGTACCCA 
GCAATGTCCT GGCCACCAGA AGACGGTTAC TCGCATCACG CGAGAGATTT CTACGACTTC 
AGGTCTTCAA ACTGGTTCGT CGAAGCGGCG ACTTCTCCTA AAGACCTGGT GATCCTCCTG 
GATGACTCCG ATGACCTGAG CTCGTCATAC TGGCGACTTG CCAAGTCCAC TGTATCAGCT 
CTCCTGGACA CCCTCGGGCC CAACGATTTC GTCAACATCT ACAGATACAG TGACACCATC 
TCCGAGCTAC ACTCCTGCTA CACCAAAGTT CTAGCTCAGG CTCTTCCAGA AACGATACGT 
GAACTGAAAA CAGCATTATG GAACGCTGAG CTCTCTGGAG GTGTGGCTAA TCTGACCGGC 
GCTCTCACCA CAGGCTTCGA AATCTTACAT CGGTACAACA GAACAGGTCT AGGATGTCAG 
TGCAATCAAG CGATCGCCGT GATAGGCGCC GGCGGGGGTG CGGGCGGGGT GAAGGGGGTG 
TTTCGCACGT GGAACTGGCC ACATATGCCT GTGCGCATCT TTACTTACCG TGTGGGAGGG 
GATGCGACTG CTGGACATAA TATGAAGGAT ATGGCCTGCA CTAATAAAGG TTTCTACGTG 
ACAATAAACG AGCACTCGGA GATCAAGTCG AAAGTGCTTC ACTTTGTGGA GGTGCTGGCG 
AGACCACTCG TCATGTACCA AACCGTGCAT CCTGTGCACT GGAGCCCCGC GTACGTTGGC 
GGCAGGAGTA GTAGCCTCGC TGATGATGGA ATGGGTCAGC TGATGTCCTC CGTCACCGTA 
CCCATCTTCG ATAGGCGAAA CCACACGCAA AGAGAAGCCA ATTTACTCGG CGTAGTTGGT 
ACCGATGTGC CTATCGATCA AATAAAGAAG CTCGTGCCAC CATATAAGTT GGGTGTGAAC 
GGTTACTCGT TCATGGTGGA TAACAACGGG CATGTATTGT ACCATCCAGA TTTAAGGCCA 
TTGCATACAA ACGAGGAATA CACTGAAACA CTGCGCCCTC TATACTCGAG TGTCGACCTC 
ACGGATGTGG AACTGCTTGT AGACTCGGAT GAGAACTCCA GGGTCAACCT AACATCACTT 
CTACTGGATC TCCGCCACGA CATGATAGAA CAGCGTGAAG GCGAAACCGA GATCGCAGTC 
AAAGTGCAAT ACGACAACAT GAGGCGCGTC GCCACCAGAC GACAGCGGTA CTTCTACAGT 
CCCGTAGACG GCACCCCGTT CTCTTTAGCC GCTGTGCTTC CTGATGGGTA TGGCATGTAT 
GAACTGCAGG CGGAGCAAGA GGTCAAGCAT AGTCCTGTTA ATGTCACGGA ATACTTCAAA 
GGCGAAAACT GGAAAGTCCA TCCTGATTGG GTGTACTGCG AATACGCGTC GACGTCTGAG 
CAGTCGTTCA ACTCAGCGGA GGAGCAGCTG GTGCACTTCC TGTCCCGCGC GGGGCGGCCG 
GGCTGGAAGT GGATGTCGCT GCGCCCGCGA GCACTCACGC TGCATAACGC GCACAAGAGG 
CAGGATAGGG ACTCCTATTA CTGTGACAAA ACTTTAGTGC AATCATTAAT TAGGGACGCA 
ATGGTAATCA AAGAATTGGA CGCTCACACT GGACATGCTA GTCATCACTC ACAACAAGGA 
CGGTTCCACA AGTTCGTGGT GACGCTGTCG TTCATCGCAA CCCGGAGCGG TCTACTGCGG 
TGGACGGAGA ACGTTAACAC CTCCAGAAGT TCTGATTCAT CTGAACCGCA CTTCAGCGAA 
AAATACGCTC GAGCCTACGA CTCGGAATGG TACCGTCGCG CAGTAGAACA CCACGCCATC 
GAACCCGAGA GCTTTGTATT CTCTGTTCCG TTCCGCGATG GCTCCGAAGC CGAGGAGTTG 
AGCGGTCGGC CGCCGCTAGT ACTAGCCACC CATGCGGTCT TCGTGGAGTC TCGCGGTCAC 
CGAGCACCCG CCGCCGTCGT CGGACTACAC TTCCAGCTAG ACTCCCTAGC AAAACACTTC 
TTGAATGTTA CTTCTGCATG TACCGGAGGC ACGGCTTGCA AGAAGACCTG CGCAGGCGAT 
GAATTGGACT GCTACATTTT GGACGACAAT GGCTTTATTA TTCTGTCAGA GGATGTCTCG 
CAAACTGGAA GGTTCTTCGG TCAGGTCGAC GGCACTATTA TGGATTCTTT AGTGCAAGAT 
CGGATATACA AAAAGGTTGT CGTCCACGAT CATCAAGGCC GATGTCCGGA TTCTAGAAAC 
TCGTTTAGTG GAGATGGTCA CAAGGTTTCG CCGCTGAAAC CATTGTCTTG GCTTGGTGGA 
TACCTAACAA GTCTAATCGC AATGTGGTTC CCTCTATGGG AACCAGTACA TGCGCGAGCC 
AGACCTTACG CTGAAGAGGA AGTCGATTAC GAAGACTACG AGAGCGAAGA TGTCGAGAAC 
GAAGACGACT TGGATAGAGA CCGTGGGACC TACGAGATCA TCATCGACGG CCTCGGCGAG 
ACCTCGCGGG ACAGCGGGCG AGCTCATCCC CCGCCCCCGT CCCCCGGCGC CGCCCCTCCC 
CCGCCGCCCC GCGAGGCCGC GCGCTCGGCA GCCGTGCGGC CTTGCGACAC CAGCGCCGAA 
CTGTTCACCC TGCAGTCTTC GAGACTCAAT ACTGCACAGC CGCTCAAGGG CAAGCTCACT 
AATTGTCACA ACTCTGGATG CGAGAGGCCG TTCAGCGTGC AGAAGATCCC GCACAGCAAC 
CTGATCCTGC TGGTGGTGGA CACGCTGTGC CCGTGCGGCG CCAAGCGCCT CGACGTGCGC 
GCGCGCGAGG CGGCGCCGCG AGCTGCCTGC CGCCGCGCGC CCGCGCTGCA CCGCCGCCGC 
CCCGCGCACT GCGGCTCCTA CCATCCCGAG GAAATCGAGA TCCAGTCATG TGGTCGCGGA 
GGACGCTTGC TGCCAGCGCT GGTGGCGGTG GTGACGCCGC TGCTGCTGCG GCTCGTGCTC 
AGCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021192477.1
CDS	828..4436
Translation	MALMAGMCPRRPLGLLLALLAAAALANQLEDIPHNEVKNWALKFGVDLWEFGRHFTKMNQIQNKYHEYNVDVVRKDGLLLVRELAAEVKNHMDFKINAVMRIMDSAEAAALSAGNGGSDGAPGSYYDARWLNVHADDGTLAARARRLALTPSRHFDHIAVNTSYSAVLMPPYINTDDGEVQNQIAWSEHLDPLFVNNYEIDPTLSWQYYASSSGFMRRYPAMSWPPEDGYSHHARDFYDFRSSNWFVEAATSPKDLVILLDDSDDLSSSYWRLAKSTVSALLDTLGPNDFVNIYRYSDTISELHSCYTKVLAQALPETIRELKTALWNAELSGGVANLTGALTTGFEILHRYNRTGLGCQCNQAIAVIGAGGGAGGVKGVFRTWNWPHMPVRIFTYRVGGDATAGHNMKDMACTNKGFYVTINEHSEIKSKVLHFVEVLARPLVMYQTVHPVHWSPAYVGGRSSSLADDGMGQLMSSVTVPIFDRRNHTQREANLLGVVGTDVPIDQIKKLVPPYKLGVNGYSFMVDNNGHVLYHPDLRPLHTNEEYTETLRPLYSSVDLTDVELLVDSDENSRVNLTSLLLDLRHDMIEQREGETEIAVKVQYDNMRRVATRRQRYFYSPVDGTPFSLAAVLPDGYGMYELQAEQEVKHSPVNVTEYFKGENWKVHPDWVYCEYASTSEQSFNSAEEQLVHFLSRAGRPGWKWMSLRPRALTLHNAHKRQDRDSYYCDKTLVQSLIRDAMVIKELDAHTGHASHHSQQGRFHKFVVTLSFIATRSGLLRWTENVNTSRSSDSSEPHFSEKYARAYDSEWYRRAVEHHAIEPESFVFSVPFRDGSEAEELSGRPPLVLATHAVFVESRGHRAPAAVVGLHFQLDSLAKHFLNVTSACTGGTACKKTCAGDELDCYILDDNGFIILSEDVSQTGRFFGQVDGTIMDSLVQDRIYKKVVVHDHQGRCPDSRNSFSGDGHKVSPLKPLSWLGGYLTSLIAMWFPLWEPVHARARPYAEEEVDYEDYESEDVENEDDLDRDRGTYEIIIDGLGETSRDSGRAHPPPPSPGAAPPPPPREAARSAAVRPCDTSAELFTLQSSRLNTAQPLKGKLTNCHNSGCERPFSVQKIPHSNLILLVVDTLCPCGAKRLDVRAREAAPRAACRRAPALHRRRPAHCGSYHPEEIEIQSCGRGGRLLPALVAVVTPLLLRLVLST

Target ORF information:

RefSeq Version	XM_021336802.1
Organism	Helicoverpa armigera(cotton bollworm)
Definition	Helicoverpa armigera voltage-dependent calcium channel subunit alpha-2/delta-3 (LOC110377785), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021336802.1

ORF Insert Sequence:

ATGGCGCTGA TGGCCGGCAT GTGTCCGCGG AGGCCGCTGG GCCTGCTGCT CGCGCTGCTC 
GCCGCCGCCG CGCTCGCCAA CCAGCTCGAA GACATCCCGC ACAATGAAGT CAAGAATTGG 
GCGCTAAAAT TCGGTGTCGA CTTATGGGAG TTTGGCCGGC ATTTCACAAA AATGAATCAA 
ATACAAAACA AATACCACGA GTACAACGTG GATGTGGTGC GCAAGGATGG GCTGCTTCTG 
GTGCGAGAGC TCGCAGCCGA AGTCAAGAAC CACATGGACT TCAAGATCAA CGCTGTCATG 
CGCATCATGG ACAGCGCTGA AGCGGCAGCT CTATCAGCCG GGAACGGCGG CAGCGATGGA 
GCACCAGGCT CGTACTATGA CGCTCGCTGG CTGAACGTGC ACGCTGATGA CGGGACCCTG 
GCTGCGAGAG CGCGACGCCT GGCGCTCACG CCGAGCCGAC ACTTCGACCA TATTGCCGTC 
AACACCAGCT ATAGTGCTGT GCTTATGCCG CCTTATATTA ACACTGATGA TGGCGAGGTA 
CAGAACCAGA TCGCGTGGTC GGAGCACTTG GACCCCCTGT TCGTGAACAA CTATGAGATC 
GACCCCACGC TTTCGTGGCA GTACTACGCC TCGTCTTCAG GATTCATGAG GCGGTACCCA 
GCAATGTCCT GGCCACCAGA AGACGGTTAC TCGCATCACG CGAGAGATTT CTACGACTTC 
AGGTCTTCAA ACTGGTTCGT CGAAGCGGCG ACTTCTCCTA AAGACCTGGT GATCCTCCTG 
GATGACTCCG ATGACCTGAG CTCGTCATAC TGGCGACTTG CCAAGTCCAC TGTATCAGCT 
CTCCTGGACA CCCTCGGGCC CAACGATTTC GTCAACATCT ACAGATACAG TGACACCATC 
TCCGAGCTAC ACTCCTGCTA CACCAAAGTT CTAGCTCAGG CTCTTCCAGA AACGATACGT 
GAACTGAAAA CAGCATTATG GAACGCTGAG CTCTCTGGAG GTGTGGCTAA TCTGACCGGC 
GCTCTCACCA CAGGCTTCGA AATCTTACAT CGGTACAACA GAACAGGTCT AGGATGTCAG 
TGCAATCAAG CGATCGCCGT GATAGGCGCC GGCGGGGGTG CGGGCGGGGT GAAGGGGGTG 
TTTCGCACGT GGAACTGGCC ACATATGCCT GTGCGCATCT TTACTTACCG TGTGGGAGGG 
GATGCGACTG CTGGACATAA TATGAAGGAT ATGGCCTGCA CTAATAAAGG TTTCTACGTG 
ACAATAAACG AGCACTCGGA GATCAAGTCG AAAGTGCTTC ACTTTGTGGA GGTGCTGGCG 
AGACCACTCG TCATGTACCA AACCGTGCAT CCTGTGCACT GGAGCCCCGC GTACGTTGGC 
GGCAGGAGTA GTAGCCTCGC TGATGATGGA ATGGGTCAGC TGATGTCCTC CGTCACCGTA 
CCCATCTTCG ATAGGCGAAA CCACACGCAA AGAGAAGCCA ATTTACTCGG CGTAGTTGGT 
ACCGATGTGC CTATCGATCA AATAAAGAAG CTCGTGCCAC CATATAAGTT GGGTGTGAAC 
GGTTACTCGT TCATGGTGGA TAACAACGGG CATGTATTGT ACCATCCAGA TTTAAGGCCA 
TTGCATACAA ACGAGGAATA CACTGAAACA CTGCGCCCTC TATACTCGAG TGTCGACCTC 
ACGGATGTGG AACTGCTTGT AGACTCGGAT GAGAACTCCA GGGTCAACCT AACATCACTT 
CTACTGGATC TCCGCCACGA CATGATAGAA CAGCGTGAAG GCGAAACCGA GATCGCAGTC 
AAAGTGCAAT ACGACAACAT GAGGCGCGTC GCCACCAGAC GACAGCGGTA CTTCTACAGT 
CCCGTAGACG GCACCCCGTT CTCTTTAGCC GCTGTGCTTC CTGATGGGTA TGGCATGTAT 
GAACTGCAGG CGGAGCAAGA GGTCAAGCAT AGTCCTGTTA ATGTCACGGA ATACTTCAAA 
GGCGAAAACT GGAAAGTCCA TCCTGATTGG GTGTACTGCG AATACGCGTC GACGTCTGAG 
CAGTCGTTCA ACTCAGCGGA GGAGCAGCTG GTGCACTTCC TGTCCCGCGC GGGGCGGCCG 
GGCTGGAAGT GGATGTCGCT GCGCCCGCGA GCACTCACGC TGCATAACGC GCACAAGAGG 
CAGGATAGGG ACTCCTATTA CTGTGACAAA ACTTTAGTGC AATCATTAAT TAGGGACGCA 
ATGGTAATCA AAGAATTGGA CGCTCACACT GGACATGCTA GTCATCACTC ACAACAAGGA 
CGGTTCCACA AGTTCGTGGT GACGCTGTCG TTCATCGCAA CCCGGAGCGG TCTACTGCGG 
TGGACGGAGA ACGTTAACAC CTCCAGAAGT TCTGATTCAT CTGAACCGCA CTTCAGCGAA 
AAATACGCTC GAGCCTACGA CTCGGAATGG TACCGTCGCG CAGTAGAACA CCACGCCATC 
GAACCCGAGA GCTTTGTATT CTCTGTTCCG TTCCGCGATG GCTCCGAAGC CGAGGAGTTG 
AGCGGTCGGC CGCCGCTAGT ACTAGCCACC CATGCGGTCT TCGTGGAGTC TCGCGGTCAC 
CGAGCACCCG CCGCCGTCGT CGGACTACAC TTCCAGCTAG ACTCCCTAGC AAAACACTTC 
TTGAATGTTA CTTCTGCATG TACCGGAGGC ACGGCTTGCA AGAAGACCTG CGCAGGCGAT 
GAATTGGACT GCTACATTTT GGACGACAAT GGCTTTATTA TTCTGTCAGA GGATGTCTCG 
CAAACTGGAA GGTTCTTCGG TCAGGTCGAC GGCACTATTA TGGATTCTTT AGTGCAAGAT 
CGGATATACA AAAAGGTTGT CGTCCACGAT CATCAAGGCC GATGTCCGGA TTCTAGAAAC 
TCGTTTAGTG GAGATGGTCA CAAGGTTTCG CCGCTGAAAC CATTGTCTTG GCTTGGTGGA 
TACCTAACAA GTCTAATCGC AATGTGGTTC CCTCTATGGG AACCAGTACA TGCGCGAGCC 
AGACCTTACG CTGAAGAGGA AGTCGATTAC GAAGACTACG AGAGCGAAGA TGTCGAGAAC 
GAAGACGACT TGGATAGAGA CCGTGGGACC TACGAGATCA TCATCGACGG CCTCGGCGAG 
ACCTCGCGGG ACAGCGGGCG AGCTCATCCC CCGCCCCCGT CCCCCGGCGC CGCCCCTCCC 
CCGCCGCCCC GCGAGGCCGC GCGCTCGGCA GCCGTGCGGC CTTGCGACAC CAGCGCCGAA 
CTGTTCACCC TGCAGTCTTC GAGACTCAAT ACTGCACAGC CGCTCAAGGG CAAGCTCACT 
AATTGTCACA ACTCTGGATG CGAGAGGCCG TTCAGCGTGC AGAAGATCCC GCACAGCAAC 
CTGATCCTGC TGGTGGTGGA CACGCTGTGC CCGTGCGGCG CCAAGCGCCT CGACGTGCGC 
GCGCGCGAGG CGGCGCCGCG AGCTGCCTGC CGCCGCGCGC CCGCGCTGCA CCGCCGCCGC 
CCCGCGCACT GCGGCTCCTA CCATCCCGAG GAAATCGAGA TCCAGTCATG TGGTCGCGGA 
GGACGCTTGC TGCCAGCGCT GGTGGCGGTG GTGACGCCGC TGCTGCTGCG GCTCGTGCTC 
AGCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110377785 ( XM_021336803.1 ) cDNA ORF clone, Helicoverpa armigera(cotton bollworm) -> XP_021192478.1 Helicoverpa armigera voltage-dependent calcium channel subunit alpha-2/delta-3 (LOC110377785), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHe209041
Clone ID Related Accession (Same CDS sequence)	XM_021336803.1
Accession Version	XM_021336803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Helicoverpa armigera(cotton bollworm)
Product	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395552.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Helicoverpa armigera Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGA TGGCCGGCAT GTGTCCGCGG AGGCCGCTGG GCCTGCTGCT CGCGCTGCTC 
GCCGCCGCCG CGCTCGCCAA CCAGCTCGAA GACATCCCGC ACAATGAAGT CAAGAATTGG 
GCGCTAAAAT TCGGTGTCGA CTTATGGGAG TTTGGCCGGC ATTTCACAAA AATGAATCAA 
ATACAAAACA AATACCACGA GTACAACGTG GATGTGGTGC GCAAGGATGG GCTGCTTCTG 
GTGCGAGAGC TCGCAGCCGA AGTCAAGAAC CACATGGACT TCAAGATCAA CGCTGTCATG 
CGCATCATGG ACAGCGCTGA AGCGGCAGCT CTATCAGCCG GGAACGGCGG CAGCGATGGA 
GCACCAGGCT CGTACTATGA CGCTCGCTGG CTGAACGTGC ACGCTGATGA CGGGACCCTG 
GCTGCGAGAG CGCGACGCCT GGCGCTCACG CCGAGCCGAC ACTTCGACCA TATTGCCGTC 
AACACCAGCT ATAGTGCTGT GCTTATGCCG CCTTATATTA ACACTGATGA TGGCGAGGTA 
CAGAACCAGA TCGCGTGGTC GGAGCACTTG GACCCCCTGT TCGTGAACAA CTATGAGATC 
GACCCCACGC TTTCGTGGCA GTACTACGCC TCGTCTTCAG GATTCATGAG GCGGTACCCA 
GCAATGTCCT GGCCACCAGA AGACGGTTAC TCGCATCACG CGAGAGATTT CTACGACTTC 
AGGTCTTCAA ACTGGTTCGT CGAAGCGGCG ACTTCTCCTA AAGACCTGGT GATCCTCCTG 
GATGACTCCG ATGACCTGAG CTCGTCATAC TGGCGACTTG CCAAGTCCAC TGTATCAGCT 
CTCCTGGACA CCCTCGGGCC CAACGATTTC GTCAACATCT ACAGATACAG TGACACCATC 
TCCGAGCTAC ACTCCTGCTA CACCAAAGTT CTAGCTCAGG CTCTTCCAGA AACGATACGT 
GAACTGAAAA CAGCATTATG GAACGCTGAG CTCTCTGGAG GTGTGGCTAA TCTGACCGGC 
GCTCTCACCA CAGGCTTCGA AATCTTACAT CGGTACAACA GAACAGGTCT AGGATGTCAG 
TGCAATCAAG CGATCGCCGT GATAGGCGCC GGCGGGGGTG CGGGCGGGGT GAAGGGGGTG 
TTTCGCACGT GGAACTGGCC ACATATGCCT GTGCGCATCT TTACTTACCG TGTGGGAGGG 
GATGCGACTG CTGGACATAA TATGAAGGAT ATGGCCTGCA CTAATAAAGG TTTCTACGTG 
ACAATAAACG AGCACTCGGA GATCAAGTCG AAAGTGCTTC ACTTTGTGGA GGTGCTGGCG 
AGACCACTCG TCATGTACCA AACCGTGCAT CCTGTGCACT GGAGCCCCGC GTACGTTGGC 
GGCAGGAGTA GTAGCCTCGC TGATGATGGA ATGGGTCAGC TGATGTCCTC CGTCACCGTA 
CCCATCTTCG ATAGGCGAAA CCACACGCAA AGAGAAGCCA ATTTACTCGG CGTAGTTGGT 
ACCGATGTGC CTATCGATCA AATAAAGAAG CTCGTGCCAC CATATAAGTT GGGTGTGAAC 
GGTTACTCGT TCATGGTGGA TAACAACGGG CATGTATTGT ACCATCCAGA TTTAAGGCCA 
TTGCATACAA ACGAGGAATA CACTGAAACA CTGCGCCCTC TATACTCGAG TGTCGACCTC 
ACGGATGTGG AACTGCTTGT AGACTCGGAT GAGAACTCCA GGGTCAACCT AACATCACTT 
CTACTGGATC TCCGCCACGA CATGATAGAA CAGCGTGAAG GCGAAACCGA GATCGCAGTC 
AAAGTGCAAT ACGACAACAT GAGGCGCGTC GCCACCAGAC GACAGCGGTA CTTCTACAGT 
CCCGTAGACG GCACCCCGTT CTCTTTAGCC GCTGTGCTTC CTGATGGGTA TGGCATGTAT 
GAACTGCAGG CGGAGCAAGA GGTCAAGCAT AGTCCTGTTA ATGTCACGGA ATACTTCAAA 
GGCGAAAACT GGAAAGTCCA TCCTGATTGG GTGTACTGCG AATACGCGTC GACGTCTGAG 
CAGTCGTTCA ACTCAGCGGA GGAGCAGCTG GTGCACTTCC TGTCCCGCGC GGGGCGGCCG 
GGCTGGAAGT GGATGTCGCT GCGCCCGCGA GCACTCACGC TGCATAACGC GCACAAGAGG 
CAGGATAGGG ACTCCTATTA CTGTGACAAA ACTTTAGTGC AATCATTAAT TAGGGACGCA 
ATGGTAATCA AAGAATTGGA CGCTCACACT GGACATGCTA GTCATCACTC ACAACAAGGA 
CGGTTCCACA AGTTCGTGGT GACGCTGTCG TTCATCGCAA CCCGGAGCGG TCTACTGCGG 
TGGACGGAGA ACGTTAACAC CTCCAGAAGT TCTGATTCAT CTGAACCGCA CTTCAGCGAA 
AAATACGCTC GAGCCTACGA CTCGGAATGG TACCGTCGCG CAGTAGAACA CCACGCCATC 
GAACCCGAGA GCTTTGTATT CTCTGTTCCG TTCCGCGATG GCTCCGAAGC CGAGGAGTTG 
AGCGGTCGGC CGCCGCTAGT ACTAGCCACC CATGCGGTCT TCGTGGAGTC TCGCGGTCAC 
CGAGCACCCG CCGCCGTCGT CGGACTACAC TTCCAGCTAG ACTCCCTAGC AAAACACTTC 
TTGAATGTTA CTTCTTGTAC CGGAGGCACG GCTTGCAAGA AGACCTGCGC AGGCGATGAA 
TTGGACTGCT ACATTTTGGA CGACAATGGC TTTATTATTC TGTCAGAGGA TGTCTCGCAA 
ACTGGAAGGT TCTTCGGTCA GGTCGACGGC ACTATTATGG ATTCTTTAGT GCAAGATCGG 
ATATACAAAA AGGTTGTCGT CCACGATCAT CAAGGCCGAT GTCCGGATTC TAGAAACTCG 
TTTAGTGGAG ATGGTCACAA GGTTTCGCCG CTGAAACCAT TGTCTTGGCT TGGTGGATAC 
CTAACAAGTC TAATCGCAAT GTGGTTCCCT CTATGGGAAC CAGTACATGC GCGAGCCAGA 
CCTTACGCTG AAGAGGAAGT CGATTACGAA GACTACGAGA GCGAAGATGT CGAGAACGAA 
GACGACTTGG ATAGAGACCG TGGGACCTAC GAGATCATCA TCGACGGCCT CGGCGAGACC 
TCGCGGGACA GCGGGCGAGC TCATCCCCCG CCCCCGTCCC CCGGCGCCGC CCCTCCCCCG 
CCGCCCCGCG AGGCCGCGCG CTCGGCAGCC GTGCGGCCTT GCGACACCAG CGCCGAACTG 
TTCACCCTGC AGTCTTCGAG ACTCAATACT GCACAGCCGC TCAAGGGCAA GCTCACTAAT 
TGTCACAACT CTGGATGCGA GAGGCCGTTC AGCGTGCAGA AGATCCCGCA CAGCAACCTG 
ATCCTGCTGG TGGTGGACAC GCTGTGCCCG TGCGGCGCCA AGCGCCTCGA CGTGCGCGCG 
CGCGAGGCGG CGCCGCGAGC TGCCTGCCGC CGCGCGCCCG CGCTGCACCG CCGCCGCCCC 
GCGCACTGCG GCTCCTACCA TCCCGAGGAA ATCGAGATCC AGTCATGTGG TCGCGGAGGA 
CGCTTGCTGC CAGCGCTGGT GGCGGTGGTG ACGCCGCTGC TGCTGCGGCT CGTGCTCAGC 
ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021192478.1
CDS	829..4434
Translation	MALMAGMCPRRPLGLLLALLAAAALANQLEDIPHNEVKNWALKFGVDLWEFGRHFTKMNQIQNKYHEYNVDVVRKDGLLLVRELAAEVKNHMDFKINAVMRIMDSAEAAALSAGNGGSDGAPGSYYDARWLNVHADDGTLAARARRLALTPSRHFDHIAVNTSYSAVLMPPYINTDDGEVQNQIAWSEHLDPLFVNNYEIDPTLSWQYYASSSGFMRRYPAMSWPPEDGYSHHARDFYDFRSSNWFVEAATSPKDLVILLDDSDDLSSSYWRLAKSTVSALLDTLGPNDFVNIYRYSDTISELHSCYTKVLAQALPETIRELKTALWNAELSGGVANLTGALTTGFEILHRYNRTGLGCQCNQAIAVIGAGGGAGGVKGVFRTWNWPHMPVRIFTYRVGGDATAGHNMKDMACTNKGFYVTINEHSEIKSKVLHFVEVLARPLVMYQTVHPVHWSPAYVGGRSSSLADDGMGQLMSSVTVPIFDRRNHTQREANLLGVVGTDVPIDQIKKLVPPYKLGVNGYSFMVDNNGHVLYHPDLRPLHTNEEYTETLRPLYSSVDLTDVELLVDSDENSRVNLTSLLLDLRHDMIEQREGETEIAVKVQYDNMRRVATRRQRYFYSPVDGTPFSLAAVLPDGYGMYELQAEQEVKHSPVNVTEYFKGENWKVHPDWVYCEYASTSEQSFNSAEEQLVHFLSRAGRPGWKWMSLRPRALTLHNAHKRQDRDSYYCDKTLVQSLIRDAMVIKELDAHTGHASHHSQQGRFHKFVVTLSFIATRSGLLRWTENVNTSRSSDSSEPHFSEKYARAYDSEWYRRAVEHHAIEPESFVFSVPFRDGSEAEELSGRPPLVLATHAVFVESRGHRAPAAVVGLHFQLDSLAKHFLNVTSCTGGTACKKTCAGDELDCYILDDNGFIILSEDVSQTGRFFGQVDGTIMDSLVQDRIYKKVVVHDHQGRCPDSRNSFSGDGHKVSPLKPLSWLGGYLTSLIAMWFPLWEPVHARARPYAEEEVDYEDYESEDVENEDDLDRDRGTYEIIIDGLGETSRDSGRAHPPPPSPGAAPPPPPREAARSAAVRPCDTSAELFTLQSSRLNTAQPLKGKLTNCHNSGCERPFSVQKIPHSNLILLVVDTLCPCGAKRLDVRAREAAPRAACRRAPALHRRRPAHCGSYHPEEIEIQSCGRGGRLLPALVAVVTPLLLRLVLST

Target ORF information:

RefSeq Version	XM_021336803.1
Organism	Helicoverpa armigera(cotton bollworm)
Definition	Helicoverpa armigera voltage-dependent calcium channel subunit alpha-2/delta-3 (LOC110377785), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021336803.1

ORF Insert Sequence:

ATGGCGCTGA TGGCCGGCAT GTGTCCGCGG AGGCCGCTGG GCCTGCTGCT CGCGCTGCTC 
GCCGCCGCCG CGCTCGCCAA CCAGCTCGAA GACATCCCGC ACAATGAAGT CAAGAATTGG 
GCGCTAAAAT TCGGTGTCGA CTTATGGGAG TTTGGCCGGC ATTTCACAAA AATGAATCAA 
ATACAAAACA AATACCACGA GTACAACGTG GATGTGGTGC GCAAGGATGG GCTGCTTCTG 
GTGCGAGAGC TCGCAGCCGA AGTCAAGAAC CACATGGACT TCAAGATCAA CGCTGTCATG 
CGCATCATGG ACAGCGCTGA AGCGGCAGCT CTATCAGCCG GGAACGGCGG CAGCGATGGA 
GCACCAGGCT CGTACTATGA CGCTCGCTGG CTGAACGTGC ACGCTGATGA CGGGACCCTG 
GCTGCGAGAG CGCGACGCCT GGCGCTCACG CCGAGCCGAC ACTTCGACCA TATTGCCGTC 
AACACCAGCT ATAGTGCTGT GCTTATGCCG CCTTATATTA ACACTGATGA TGGCGAGGTA 
CAGAACCAGA TCGCGTGGTC GGAGCACTTG GACCCCCTGT TCGTGAACAA CTATGAGATC 
GACCCCACGC TTTCGTGGCA GTACTACGCC TCGTCTTCAG GATTCATGAG GCGGTACCCA 
GCAATGTCCT GGCCACCAGA AGACGGTTAC TCGCATCACG CGAGAGATTT CTACGACTTC 
AGGTCTTCAA ACTGGTTCGT CGAAGCGGCG ACTTCTCCTA AAGACCTGGT GATCCTCCTG 
GATGACTCCG ATGACCTGAG CTCGTCATAC TGGCGACTTG CCAAGTCCAC TGTATCAGCT 
CTCCTGGACA CCCTCGGGCC CAACGATTTC GTCAACATCT ACAGATACAG TGACACCATC 
TCCGAGCTAC ACTCCTGCTA CACCAAAGTT CTAGCTCAGG CTCTTCCAGA AACGATACGT 
GAACTGAAAA CAGCATTATG GAACGCTGAG CTCTCTGGAG GTGTGGCTAA TCTGACCGGC 
GCTCTCACCA CAGGCTTCGA AATCTTACAT CGGTACAACA GAACAGGTCT AGGATGTCAG 
TGCAATCAAG CGATCGCCGT GATAGGCGCC GGCGGGGGTG CGGGCGGGGT GAAGGGGGTG 
TTTCGCACGT GGAACTGGCC ACATATGCCT GTGCGCATCT TTACTTACCG TGTGGGAGGG 
GATGCGACTG CTGGACATAA TATGAAGGAT ATGGCCTGCA CTAATAAAGG TTTCTACGTG 
ACAATAAACG AGCACTCGGA GATCAAGTCG AAAGTGCTTC ACTTTGTGGA GGTGCTGGCG 
AGACCACTCG TCATGTACCA AACCGTGCAT CCTGTGCACT GGAGCCCCGC GTACGTTGGC 
GGCAGGAGTA GTAGCCTCGC TGATGATGGA ATGGGTCAGC TGATGTCCTC CGTCACCGTA 
CCCATCTTCG ATAGGCGAAA CCACACGCAA AGAGAAGCCA ATTTACTCGG CGTAGTTGGT 
ACCGATGTGC CTATCGATCA AATAAAGAAG CTCGTGCCAC CATATAAGTT GGGTGTGAAC 
GGTTACTCGT TCATGGTGGA TAACAACGGG CATGTATTGT ACCATCCAGA TTTAAGGCCA 
TTGCATACAA ACGAGGAATA CACTGAAACA CTGCGCCCTC TATACTCGAG TGTCGACCTC 
ACGGATGTGG AACTGCTTGT AGACTCGGAT GAGAACTCCA GGGTCAACCT AACATCACTT 
CTACTGGATC TCCGCCACGA CATGATAGAA CAGCGTGAAG GCGAAACCGA GATCGCAGTC 
AAAGTGCAAT ACGACAACAT GAGGCGCGTC GCCACCAGAC GACAGCGGTA CTTCTACAGT 
CCCGTAGACG GCACCCCGTT CTCTTTAGCC GCTGTGCTTC CTGATGGGTA TGGCATGTAT 
GAACTGCAGG CGGAGCAAGA GGTCAAGCAT AGTCCTGTTA ATGTCACGGA ATACTTCAAA 
GGCGAAAACT GGAAAGTCCA TCCTGATTGG GTGTACTGCG AATACGCGTC GACGTCTGAG 
CAGTCGTTCA ACTCAGCGGA GGAGCAGCTG GTGCACTTCC TGTCCCGCGC GGGGCGGCCG 
GGCTGGAAGT GGATGTCGCT GCGCCCGCGA GCACTCACGC TGCATAACGC GCACAAGAGG 
CAGGATAGGG ACTCCTATTA CTGTGACAAA ACTTTAGTGC AATCATTAAT TAGGGACGCA 
ATGGTAATCA AAGAATTGGA CGCTCACACT GGACATGCTA GTCATCACTC ACAACAAGGA 
CGGTTCCACA AGTTCGTGGT GACGCTGTCG TTCATCGCAA CCCGGAGCGG TCTACTGCGG 
TGGACGGAGA ACGTTAACAC CTCCAGAAGT TCTGATTCAT CTGAACCGCA CTTCAGCGAA 
AAATACGCTC GAGCCTACGA CTCGGAATGG TACCGTCGCG CAGTAGAACA CCACGCCATC 
GAACCCGAGA GCTTTGTATT CTCTGTTCCG TTCCGCGATG GCTCCGAAGC CGAGGAGTTG 
AGCGGTCGGC CGCCGCTAGT ACTAGCCACC CATGCGGTCT TCGTGGAGTC TCGCGGTCAC 
CGAGCACCCG CCGCCGTCGT CGGACTACAC TTCCAGCTAG ACTCCCTAGC AAAACACTTC 
TTGAATGTTA CTTCTTGTAC CGGAGGCACG GCTTGCAAGA AGACCTGCGC AGGCGATGAA 
TTGGACTGCT ACATTTTGGA CGACAATGGC TTTATTATTC TGTCAGAGGA TGTCTCGCAA 
ACTGGAAGGT TCTTCGGTCA GGTCGACGGC ACTATTATGG ATTCTTTAGT GCAAGATCGG 
ATATACAAAA AGGTTGTCGT CCACGATCAT CAAGGCCGAT GTCCGGATTC TAGAAACTCG 
TTTAGTGGAG ATGGTCACAA GGTTTCGCCG CTGAAACCAT TGTCTTGGCT TGGTGGATAC 
CTAACAAGTC TAATCGCAAT GTGGTTCCCT CTATGGGAAC CAGTACATGC GCGAGCCAGA 
CCTTACGCTG AAGAGGAAGT CGATTACGAA GACTACGAGA GCGAAGATGT CGAGAACGAA 
GACGACTTGG ATAGAGACCG TGGGACCTAC GAGATCATCA TCGACGGCCT CGGCGAGACC 
TCGCGGGACA GCGGGCGAGC TCATCCCCCG CCCCCGTCCC CCGGCGCCGC CCCTCCCCCG 
CCGCCCCGCG AGGCCGCGCG CTCGGCAGCC GTGCGGCCTT GCGACACCAG CGCCGAACTG 
TTCACCCTGC AGTCTTCGAG ACTCAATACT GCACAGCCGC TCAAGGGCAA GCTCACTAAT 
TGTCACAACT CTGGATGCGA GAGGCCGTTC AGCGTGCAGA AGATCCCGCA CAGCAACCTG 
ATCCTGCTGG TGGTGGACAC GCTGTGCCCG TGCGGCGCCA AGCGCCTCGA CGTGCGCGCG 
CGCGAGGCGG CGCCGCGAGC TGCCTGCCGC CGCGCGCCCG CGCTGCACCG CCGCCGCCCC 
GCGCACTGCG GCTCCTACCA TCCCGAGGAA ATCGAGATCC AGTCATGTGG TCGCGGAGGA 
CGCTTGCTGC CAGCGCTGGT GGCGGTGGTG ACGCCGCTGC TGCTGCGGCT CGTGCTCAGC 
ACGTGA