tbc1d8b cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder)

Gene
Clones
gRNAs

Summary

Gene Symbol	tbc1d8b
Entrez Gene ID	102294662
Full Name	TBC1 domain family member 8B
Gene Type	protein-coding
Organism	Haplochromis burtoni(Burton's mouthbrooder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005928487.1	XP_005928549.1	TBC1 domain family member 8B isoform X1
XM_005928488.1	XP_005928550.1	TBC1 domain family member 8B isoform X2

Pathways from BioSystems
Gene Ontology

The following tbc1d8b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tbc1d8b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHa14883	XM_005928487.1 Latest version!	Haplochromis burtoni TBC1 domain family, member 8B (with GRAM domain) (tbc1d8b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OHa14884	XM_005928488.1 Latest version!	Haplochromis burtoni TBC1 domain family, member 8B (with GRAM domain) (tbc1d8b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tbc1d8b ( XM_005928487.1 ) cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder) -> XP_005928549.1 Haplochromis burtoni TBC1 domain family, member 8B (with GRAM domain) (tbc1d8b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHa14883
Clone ID Related Accession (Same CDS sequence)	XM_005928487.1
Accession Version	XM_005928487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-28
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Product	TBC1 domain family member 8B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005179497.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Haplochromis burtoni Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTCA AACCCGAAGA GATTCTGCTG AAAAATGCTT TTAAATTGTG GGTCACCGAG 
AAGGATAACG AGTATTTCGT GCTGCAGAGG AGACGAGGGT ACGGAGAAGG TGGTGGCGGT 
TTGACAGGCC TCCTTGTAGG AACCCTGGAT ACTGTGTTGG ACTCCACTTC CAAAGTGGCC 
CCTTATCGCA TTCTGCACCA CACGCCAGAC TCACAAGTGT ACTGGTCAAT AGCATGCGGT 
GTCACCAAAG AGGAGATTAT TCAGCACTGG GATTGGCTGC AGCAGAACAT TATGAGGACG 
CTCTCTGTTT TTGACTCCAG CGAAGACGTC ACCAGCTTTG TACAGGGCAA GATTAGAGGT 
CTTATTGCAG AAGAGGGGAA GACATGCTTA GTGCAGGAGG ATGATCCCGA GAAGTTTCGA 
GAAGCCCACT TGAGGTTTGA GAAGTGGTTT GAGCTGCCAC CAGAGGAAAA GCTGGTCACA 
TATTACTCAT GCAGCTACTG GAAAGGAAAA GTACCCTGCC AGGGCTGGCT CTACCTCAGC 
ACAAACTTCC TGTGCTTCTA TTCATACCTT CTGGGAGCTG AGGTTAAACT AGTCATCTCC 
TGGGATGAGC TTTGGAGGCT GGAGAAAACC TCTAATGTCA TTCTGACTGA GAGCATCCAT 
GTTTTGGCTC ATGGAGAGGA TCACTACTTC TCCATGCTGC TGCACCTGAA CGAAACGTTC 
GTTATCATGG AACAGTTGGC TGACTACTCC ATCAAACGTC TTTTTGATAA AGAGTCCTTC 
CAGAGAGAGC CAGCGCTGTC TGACCCGCTG CAGATTACAA AGAGAGGGCT AGAAGCTAAT 
GCAAAGAGTG AGCAATTCAG GACATTTTTC AGGCTTCCCA AAGAGGAAAA CCTGTTAGAG 
GTGCACGAGA GCTTTTTGTG GGTTCCCTTC AGCCATTTTA ACACGCTTGG CAAGATTTGT 
CTGTCGGAGA ACTACCTGTG TTTTGCCAGT CAGGATGGAA GTCAATGCCA TGTCATTATT 
CCAATGCGAG AGGTTGTTAA CGTTGAGAAA CCAGACTCCA GCAGCAGAGC TTTAACGGTG 
TGCGTGCGCG GCAAGAGGGC GCTGCGTTTC TCTGAGGTGC GGGACTATCA GCGGCTTGCC 
AATACAATCC GTAGTAGGTG TGGGATTAGT GCCAGCCCAC AGCACTCTGC TTCATCAGAG 
GCCATACGGG GAGAATGTCA GTCTCTCATA AACCACTTTG AGGACAATCC AGAGGACGTA 
ACTTTAATGG TGGGGCAGAA AGACAGCAGC AAGGCCGTGA GCACCGAGGC TCTCATGACT 
GTTTTCCACC CCCAGGATGT TGAAAACCTT GATCCCAAAA TGCTTAAGGA GAAGATGAAG 
GAGCAGTCTT GGAACATCCA TTTCTCTGAG TATGGACGTG GTACGAGTAT GTTCTTTACC 
AGGAAGACGC GAGACTTGAT TGTGCGCGGT GTTCCTGAGG CCTTGAGGGG AGAGCTGTGG 
ATGCTTTTCT CAGGGGCTGT AAACGACATG GCCACTCACC CCGGCTATTA CACTGAGCTG 
GTTGAACAGT CTCTGGGCAC AAGCACTCTG GCTACAGATG AGATTGAAAG AGACCTGCAC 
CGCTCTCTCC CTGAGCACCC GGCCTTTCAG AGCGACACAG GAATCTCAGC TCTTCGCAGA 
GTCCTTACTG CCTATGCCTA CAGGAACCCT AAAATTGGAT ACTGCCAGGC TATGAACATT 
CTCACCTCGG TTCTCCTGCT GTATGCAAAA GAAGAGGAGG CTTTCTGGCT GCTGGTAGCC 
GTCTGTGAGA GGATGCTGCC AGATTACTTC AACCGCCGAA TTATTGGTGC TTTGGTTGAC 
CAGGCTGTGT TCGAAGATCT GATTAGAGAA AACCTCCCCC AGCTGGTGGA ACACATGACA 
GACCTGAGCT TCTTCTCCTC CGTGTCTCTG TCCTGGTTTC TCACGCTTTT CATCAGTGTG 
CTGCCTATAG AGAGCGCCGT GAACGTGGTG GATTGTTTTT TTTATGATGG AATCAAAGCC 
ATCCTGCAGC TTGGCCTGGC CGTGCTGGAC TACAACATGG AGGCTCTGAT CAGCTGCCAC 
GACGATGCAG AAGCTGTCAC CATCCTCAAC AAGTTTTTTG ACAGTGTGAC AAACAAAGAC 
AGCCCATTGC CCCCGACAGT GCAGCAAGCT TCAGTAGGCA ATAATGATAA ATCATCTCAC 
ATCAGCGTAG ATATCAGTGA GCTGATCCGA GAGGCCTATG AGAAATATGG AAATATTCGT 
TCAGAGGAAG TTGAGAGTTC ACGGAAAAGA AACAAACTAT ATGTGATTCA AACACTGGAG 
GACACAACCA AGCAAAACGT GATCCGTGTG GTGTCCCAAG ATGTCAGATT CAGTGCCTCA 
CAACTGGACG AGCTTTATAA TTTATTCAAA AGGCAGCATT TCCTCAGCTG CTACTGGACA 
ATGAACAGCC CAGCTCTGCT CCATCATGAC CCCAGTCTGG CCTATCTGGA GCAGTACCAG 
CTGGGTTTCC AACAGTTCAG CATGCTCTTC TCCCTGCTGG AGCCCTGGGC TTTCTGCAGC 
ATCAAGAGCA CCCTCTCCCT CTGGGTTTTC CGCCTGATAG ATGAGAACCA GGATGGTCTC 
ATTAACTTTA AAGAATTCTG CTGTGCCCTT GATACTTTAT ACACCGGATC TTTCACAAAT 
AAGTTGAAAT TTCTCTTCAA GCTCCACCTG CCACCAGCCT TCACAGGTAG TCCTTTACAC 
TTAAAGGAAC AAAGAATCCA GCACTTTATT CCAGTGACTG AAGATTCTTC TCACTTGAGT 
AGACTCACTG CCTTTGATCT TCCAGAAGAT GGTGTGATAA GGAAAAGCCC TGAAAGAGGA 
AGAGGGAAGG TTGACCTGCA GGCCTATCTG AAACAATGGC AGAATGAAAT ATTAAAGAAA 
GAGGAAACCA TCAAAGACCT GCCCAGAATT AATCAGACTC AGTTCATCCA GTTCTCCAAG 
ACCCTGTACA ACATTTTTCA TGGTGATCCC GAGGAGGAGT CACTGTACCG AGCCGTGGCT 
CATGTGACCA GCCTCCTCCT GAGGATGGAA GAGGTGGGGC GGAGGCTGCA GGAGCCGAGC 
AGCCCACCCG AGTCCAAAAA GCCTGCCAAT ACCACTGCTG GCGAGGAGTC TTCAACTGAA 
GAGTCCTCCA CAACCCCAGA GAGCTCTGAC ACTTCCAGCT CCCACAACAG TCAAGACCTC 
TCAGAGACCG AGTGGTCGTT TTCCTTCGAG CAGGTCCTAG CATCTCTGCT CAATGAACCG 
GCCATAGTCA GCTTCTTTGA GAGGCCTGTT GATATTCAGA TGAAGCTGGG ACAAGCTAAG 
GTCGCCCAGC TGAAGTCAAA GACCAATAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005928549.1
CDS	143..3535
Translation	MWLKPEEILLKNAFKLWVTEKDNEYFVLQRRRGYGEGGGGLTGLLVGTLDTVLDSTSKVAPYRILHHTPDSQVYWSIACGVTKEEIIQHWDWLQQNIMRTLSVFDSSEDVTSFVQGKIRGLIAEEGKTCLVQEDDPEKFREAHLRFEKWFELPPEEKLVTYYSCSYWKGKVPCQGWLYLSTNFLCFYSYLLGAEVKLVISWDELWRLEKTSNVILTESIHVLAHGEDHYFSMLLHLNETFVIMEQLADYSIKRLFDKESFQREPALSDPLQITKRGLEANAKSEQFRTFFRLPKEENLLEVHESFLWVPFSHFNTLGKICLSENYLCFASQDGSQCHVIIPMREVVNVEKPDSSSRALTVCVRGKRALRFSEVRDYQRLANTIRSRCGISASPQHSASSEAIRGECQSLINHFEDNPEDVTLMVGQKDSSKAVSTEALMTVFHPQDVENLDPKMLKEKMKEQSWNIHFSEYGRGTSMFFTRKTRDLIVRGVPEALRGELWMLFSGAVNDMATHPGYYTELVEQSLGTSTLATDEIERDLHRSLPEHPAFQSDTGISALRRVLTAYAYRNPKIGYCQAMNILTSVLLLYAKEEEAFWLLVAVCERMLPDYFNRRIIGALVDQAVFEDLIRENLPQLVEHMTDLSFFSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAILQLGLAVLDYNMEALISCHDDAEAVTILNKFFDSVTNKDSPLPPTVQQASVGNNDKSSHISVDISELIREAYEKYGNIRSEEVESSRKRNKLYVIQTLEDTTKQNVIRVVSQDVRFSASQLDELYNLFKRQHFLSCYWTMNSPALLHHDPSLAYLEQYQLGFQQFSMLFSLLEPWAFCSIKSTLSLWVFRLIDENQDGLINFKEFCCALDTLYTGSFTNKLKFLFKLHLPPAFTGSPLHLKEQRIQHFIPVTEDSSHLSRLTAFDLPEDGVIRKSPERGRGKVDLQAYLKQWQNEILKKEETIKDLPRINQTQFIQFSKTLYNIFHGDPEEESLYRAVAHVTSLLLRMEEVGRRLQEPSSPPESKKPANTTAGEESSTEESSTTPESSDTSSSHNSQDLSETEWSFSFEQVLASLLNEPAIVSFFERPVDIQMKLGQAKVAQLKSKTNK

Target ORF information:

RefSeq Version	XM_005928487.1
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Definition	Haplochromis burtoni TBC1 domain family, member 8B (with GRAM domain) (tbc1d8b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005928487.1

ORF Insert Sequence:

ATGTGGCTCA AACCCGAAGA GATTCTGCTG AAAAATGCTT TTAAATTGTG GGTCACCGAG 
AAGGATAACG AGTATTTCGT GCTGCAGAGG AGACGAGGGT ACGGAGAAGG TGGTGGCGGT 
TTGACAGGCC TCCTTGTAGG AACCCTGGAT ACTGTGTTGG ACTCCACTTC CAAAGTGGCC 
CCTTATCGCA TTCTGCACCA CACGCCAGAC TCACAAGTGT ACTGGTCAAT AGCATGCGGT 
GTCACCAAAG AGGAGATTAT TCAGCACTGG GATTGGCTGC AGCAGAACAT TATGAGGACG 
CTCTCTGTTT TTGACTCCAG CGAAGACGTC ACCAGCTTTG TACAGGGCAA GATTAGAGGT 
CTTATTGCAG AAGAGGGGAA GACATGCTTA GTGCAGGAGG ATGATCCCGA GAAGTTTCGA 
GAAGCCCACT TGAGGTTTGA GAAGTGGTTT GAGCTGCCAC CAGAGGAAAA GCTGGTCACA 
TATTACTCAT GCAGCTACTG GAAAGGAAAA GTACCCTGCC AGGGCTGGCT CTACCTCAGC 
ACAAACTTCC TGTGCTTCTA TTCATACCTT CTGGGAGCTG AGGTTAAACT AGTCATCTCC 
TGGGATGAGC TTTGGAGGCT GGAGAAAACC TCTAATGTCA TTCTGACTGA GAGCATCCAT 
GTTTTGGCTC ATGGAGAGGA TCACTACTTC TCCATGCTGC TGCACCTGAA CGAAACGTTC 
GTTATCATGG AACAGTTGGC TGACTACTCC ATCAAACGTC TTTTTGATAA AGAGTCCTTC 
CAGAGAGAGC CAGCGCTGTC TGACCCGCTG CAGATTACAA AGAGAGGGCT AGAAGCTAAT 
GCAAAGAGTG AGCAATTCAG GACATTTTTC AGGCTTCCCA AAGAGGAAAA CCTGTTAGAG 
GTGCACGAGA GCTTTTTGTG GGTTCCCTTC AGCCATTTTA ACACGCTTGG CAAGATTTGT 
CTGTCGGAGA ACTACCTGTG TTTTGCCAGT CAGGATGGAA GTCAATGCCA TGTCATTATT 
CCAATGCGAG AGGTTGTTAA CGTTGAGAAA CCAGACTCCA GCAGCAGAGC TTTAACGGTG 
TGCGTGCGCG GCAAGAGGGC GCTGCGTTTC TCTGAGGTGC GGGACTATCA GCGGCTTGCC 
AATACAATCC GTAGTAGGTG TGGGATTAGT GCCAGCCCAC AGCACTCTGC TTCATCAGAG 
GCCATACGGG GAGAATGTCA GTCTCTCATA AACCACTTTG AGGACAATCC AGAGGACGTA 
ACTTTAATGG TGGGGCAGAA AGACAGCAGC AAGGCCGTGA GCACCGAGGC TCTCATGACT 
GTTTTCCACC CCCAGGATGT TGAAAACCTT GATCCCAAAA TGCTTAAGGA GAAGATGAAG 
GAGCAGTCTT GGAACATCCA TTTCTCTGAG TATGGACGTG GTACGAGTAT GTTCTTTACC 
AGGAAGACGC GAGACTTGAT TGTGCGCGGT GTTCCTGAGG CCTTGAGGGG AGAGCTGTGG 
ATGCTTTTCT CAGGGGCTGT AAACGACATG GCCACTCACC CCGGCTATTA CACTGAGCTG 
GTTGAACAGT CTCTGGGCAC AAGCACTCTG GCTACAGATG AGATTGAAAG AGACCTGCAC 
CGCTCTCTCC CTGAGCACCC GGCCTTTCAG AGCGACACAG GAATCTCAGC TCTTCGCAGA 
GTCCTTACTG CCTATGCCTA CAGGAACCCT AAAATTGGAT ACTGCCAGGC TATGAACATT 
CTCACCTCGG TTCTCCTGCT GTATGCAAAA GAAGAGGAGG CTTTCTGGCT GCTGGTAGCC 
GTCTGTGAGA GGATGCTGCC AGATTACTTC AACCGCCGAA TTATTGGTGC TTTGGTTGAC 
CAGGCTGTGT TCGAAGATCT GATTAGAGAA AACCTCCCCC AGCTGGTGGA ACACATGACA 
GACCTGAGCT TCTTCTCCTC CGTGTCTCTG TCCTGGTTTC TCACGCTTTT CATCAGTGTG 
CTGCCTATAG AGAGCGCCGT GAACGTGGTG GATTGTTTTT TTTATGATGG AATCAAAGCC 
ATCCTGCAGC TTGGCCTGGC CGTGCTGGAC TACAACATGG AGGCTCTGAT CAGCTGCCAC 
GACGATGCAG AAGCTGTCAC CATCCTCAAC AAGTTTTTTG ACAGTGTGAC AAACAAAGAC 
AGCCCATTGC CCCCGACAGT GCAGCAAGCT TCAGTAGGCA ATAATGATAA ATCATCTCAC 
ATCAGCGTAG ATATCAGTGA GCTGATCCGA GAGGCCTATG AGAAATATGG AAATATTCGT 
TCAGAGGAAG TTGAGAGTTC ACGGAAAAGA AACAAACTAT ATGTGATTCA AACACTGGAG 
GACACAACCA AGCAAAACGT GATCCGTGTG GTGTCCCAAG ATGTCAGATT CAGTGCCTCA 
CAACTGGACG AGCTTTATAA TTTATTCAAA AGGCAGCATT TCCTCAGCTG CTACTGGACA 
ATGAACAGCC CAGCTCTGCT CCATCATGAC CCCAGTCTGG CCTATCTGGA GCAGTACCAG 
CTGGGTTTCC AACAGTTCAG CATGCTCTTC TCCCTGCTGG AGCCCTGGGC TTTCTGCAGC 
ATCAAGAGCA CCCTCTCCCT CTGGGTTTTC CGCCTGATAG ATGAGAACCA GGATGGTCTC 
ATTAACTTTA AAGAATTCTG CTGTGCCCTT GATACTTTAT ACACCGGATC TTTCACAAAT 
AAGTTGAAAT TTCTCTTCAA GCTCCACCTG CCACCAGCCT TCACAGGTAG TCCTTTACAC 
TTAAAGGAAC AAAGAATCCA GCACTTTATT CCAGTGACTG AAGATTCTTC TCACTTGAGT 
AGACTCACTG CCTTTGATCT TCCAGAAGAT GGTGTGATAA GGAAAAGCCC TGAAAGAGGA 
AGAGGGAAGG TTGACCTGCA GGCCTATCTG AAACAATGGC AGAATGAAAT ATTAAAGAAA 
GAGGAAACCA TCAAAGACCT GCCCAGAATT AATCAGACTC AGTTCATCCA GTTCTCCAAG 
ACCCTGTACA ACATTTTTCA TGGTGATCCC GAGGAGGAGT CACTGTACCG AGCCGTGGCT 
CATGTGACCA GCCTCCTCCT GAGGATGGAA GAGGTGGGGC GGAGGCTGCA GGAGCCGAGC 
AGCCCACCCG AGTCCAAAAA GCCTGCCAAT ACCACTGCTG GCGAGGAGTC TTCAACTGAA 
GAGTCCTCCA CAACCCCAGA GAGCTCTGAC ACTTCCAGCT CCCACAACAG TCAAGACCTC 
TCAGAGACCG AGTGGTCGTT TTCCTTCGAG CAGGTCCTAG CATCTCTGCT CAATGAACCG 
GCCATAGTCA GCTTCTTTGA GAGGCCTGTT GATATTCAGA TGAAGCTGGG ACAAGCTAAG 
GTCGCCCAGC TGAAGTCAAA GACCAATAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d8b ( XM_005928488.1 ) cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder) -> XP_005928550.1 Haplochromis burtoni TBC1 domain family, member 8B (with GRAM domain) (tbc1d8b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHa14884
Clone ID Related Accession (Same CDS sequence)	XM_005928488.1
Accession Version	XM_005928488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-28
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Product	TBC1 domain family member 8B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005179497.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Haplochromis burtoni Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTCA AACCCGAAGA GATTCTGCTG AAAAATGCTT TTAAATTGTG GGTCACCGAG 
AAGGATAACG AGTATTTCGT GCTGCAGAGG AGACGAGGGT ACGGAGAAGG TGGTGGCGGT 
TTGACAGGCC TCCTTGTAGG AACCCTGGAT ACTGTGTTGG ACTCCACTTC CAAAGTGGCC 
CCTTATCGCA TTCTGCACCA CACGCCAGAC TCACAAGTGT ACTGGTCAAT AGCATGCGGT 
GTCACCAAAG AGGAGATTAT TCAGCACTGG GATTGGCTGC AGCAGAACAT TATGAGGACG 
CTCTCTGTTT TTGACTCCAG CGAAGACGTC ACCAGCTTTG TACAGGGCAA GATTAGAGGT 
CTTATTGCAG AAGAGGGGAA GACATGCTTA GTGCAGGAGG ATGATCCCGA GAAGTTTCGA 
GAAGCCCACT TGAGGTTTGA GAAGTGGTTT GAGCTGCCAC CAGAGGAAAA GCTGGTCACA 
TATTACTCAT GCAGCTACTG GAAAGGAAAA GTACCCTGCC AGGGCTGGCT CTACCTCAGC 
ACAAACTTCC TGTGCTTCTA TTCATACCTT CTGGGAGCTG AGGTTAAACT AGTCATCTCC 
TGGGATGAGC TTTGGAGGCT GGAGAAAACC TCTAATGTCA TTCTGACTGA GAGCATCCAT 
GTTTTGGCTC ATGGAGAGGA TCACTACTTC TCCATGCTGC TGCACCTGAA CGAAACGTTC 
GTTATCATGG AACAGTTGGC TGACTACTCC ATCAAACGTC TTTTTGATAA AGAGTCCTTC 
CAGAGAGAGC CAGCGCTGTC TGACCCGCTG CAGATTACAA AGAGAGGGCT AGAAGCTAAT 
GCAAAGAGTG AGCAATTCAG GACATTTTTC AGGCTTCCCA AAGAGGAAAA CCTGTTAGAG 
GTGCACGAGA GCTTTTTGTG GGTTCCCTTC AGCCATTTTA ACACGCTTGG CAAGATTTGT 
CTGTCGGAGA ACTACCTGTG TTTTGCCAGT CAGGATGGAA GTCAATGCCA TGTCATTATT 
CCAATGCGAG AGGTTGTTAA CGTTGAGAAA CCAGACTCCA GCAGCAGAGC TTTAACGGTG 
TGCGTGCGCG GCAAGAGGGC GCTGCGTTTC TCTGAGGTGC GGGACTATCA GCGGCTTGCC 
AATACAATCC GTAGTAGGTG TGGGATTAGT GCCAGCCCAC AGCACTCTGC TTCATCAGAG 
GCCATACGGG GAGAATGTCA GTCTCTCATA AACCACTTTG AGGACAATCC AGAGGACGTA 
ACTTTAATGG TGGGGCAGAA AGACAGCAGC AAGGCCGTGA GCACCGAGGC TCTCATGACT 
GTTTTCCACC CCCAGGATGT TGAAAACCTT GATCCCAAAA TGCTTAAGGA GAAGATGAAG 
GAGCAGTCTT GGAACATCCA TTTCTCTGAG TATGGACGTG GTACGAGTAT GTTCTTTACC 
AGGAAGACGC GAGACTTGAT TGTGCGCGGT GTTCCTGAGG CCTTGAGGGG AGAGCTGTGG 
ATGCTTTTCT CAGGGGCTGT AAACGACATG GCCACTCACC CCGGCTATTA CACTGAGCTG 
GTTGAACAGT CTCTGGGCAC AAGCACTCTG GCTACAGATG AGATTGAAAG AGACCTGCAC 
CGCTCTCTCC CTGAGCACCC GGCCTTTCAG AGCGACACAG GAATCTCAGC TCTTCGCAGA 
GTCCTTACTG CCTATGCCTA CAGGAACCCT AAAATTGGAT ACTGCCAGGC TATGAACATT 
CTCACCTCGG TTCTCCTGCT GTATGCAAAA GAAGAGGAGG CTTTCTGGCT GCTGGTAGCC 
GTCTGTGAGA GGATGCTGCC AGATTACTTC AACCGCCGAA TTATTGGTGC TTTGGTTGAC 
CAGGCTGTGT TCGAAGATCT GATTAGAGAA AACCTCCCCC AGCTGGTGGA ACACATGACA 
GACCTGAGCT TCTTCTCCTC CGTGTCTCTG TCCTGGTTTC TCACGCTTTT CATCAGTGTG 
CTGCCTATAG AGAGCGCCGT GAACGTGGTG GATTGTTTTT TTTATGATGG AATCAAAGCC 
ATCCTGCAGC TTGGCCTGGC CGTGCTGGAC TACAACATGG AGGCTCTGAT CAGCTGCCAC 
GACGATGCAG AAGCTGTCAC CATCCTCAAC AAGTTTTTTG ACAGTGTGAC AAACAAAGAC 
AGCCCATTGC CCCCGACAGT GCAGCAAGCT TCAGTAGGCA ATAATGATAA ATCATCTCAC 
ATCAGCGTAG ATATCAGTGA GCTGATCCGA GAGGCCTATG AGAAATATGG AAATATTCGT 
TCAGAGGAAG TTGAGAGTTC ACGGAAAAGA AACAAACTAT ATGTGATTCA AACACTGGAG 
GACACAACCA AGCAAAACGT GATCCGTGTG GTGTCCCAAG ATGTCAGATT CAGTGCCTCA 
CAACTGGACG AGCTTTATAA TTTATTCAAA AGGCAGCATT TCCTCAGCTG CTACTGGACA 
ATGAACAGCC CAGCTCTGCT CCATCATGAC CCCAGTCTGG CCTATCTGGA GCAGTACCAG 
CTGGGTTTCC AACAGTTCAG CATGCTCTTC TCCCTGCTGG AGCCCTGGGC TTTCTGCAGC 
ATCAAGAGCA CCCTCTCCCT CTGGGTTTTC CGCCTGATAG ATGAGAACCA GGATGGTCTC 
ATTAACTTTA AAGAATTCTG CTGTGCCCTT GATACTTTAT ACACCGGATC TTTCACAAAT 
AAGTTGAAAT TTCTCTTCAA GCTCCACCTG CCACCAGCCT TTGATCTTCC AGAAGATGGT 
GTGATAAGGA AAAGCCCTGA AAGAGGAAGA GGGAAGGTTG ACCTGCAGGC CTATCTGAAA 
CAATGGCAGA ATGAAATATT AAAGAAAGAG GAAACCATCA AAGACCTGCC CAGAATTAAT 
CAGACTCAGT TCATCCAGTT CTCCAAGACC CTGTACAACA TTTTTCATGG TGATCCCGAG 
GAGGAGTCAC TGTACCGAGC CGTGGCTCAT GTGACCAGCC TCCTCCTGAG GATGGAAGAG 
GTGGGGCGGA GGCTGCAGGA GCCGAGCAGC CCACCCGAGT CCAAAAAGCC TGCCAATACC 
ACTGCTGGCG AGGAGTCTTC AACTGAAGAG TCCTCCACAA CCCCAGAGAG CTCTGACACT 
TCCAGCTCCC ACAACAGTCA AGACCTCTCA GAGACCGAGT GGTCGTTTTC CTTCGAGCAG 
GTCCTAGCAT CTCTGCTCAA TGAACCGGCC ATAGTCAGCT TCTTTGAGAG GCCTGTTGAT 
ATTCAGATGA AGCTGGGACA AGCTAAGGTC GCCCAGCTGA AGTCAAAGAC CAATAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005928550.1
CDS	143..3442
Translation	MWLKPEEILLKNAFKLWVTEKDNEYFVLQRRRGYGEGGGGLTGLLVGTLDTVLDSTSKVAPYRILHHTPDSQVYWSIACGVTKEEIIQHWDWLQQNIMRTLSVFDSSEDVTSFVQGKIRGLIAEEGKTCLVQEDDPEKFREAHLRFEKWFELPPEEKLVTYYSCSYWKGKVPCQGWLYLSTNFLCFYSYLLGAEVKLVISWDELWRLEKTSNVILTESIHVLAHGEDHYFSMLLHLNETFVIMEQLADYSIKRLFDKESFQREPALSDPLQITKRGLEANAKSEQFRTFFRLPKEENLLEVHESFLWVPFSHFNTLGKICLSENYLCFASQDGSQCHVIIPMREVVNVEKPDSSSRALTVCVRGKRALRFSEVRDYQRLANTIRSRCGISASPQHSASSEAIRGECQSLINHFEDNPEDVTLMVGQKDSSKAVSTEALMTVFHPQDVENLDPKMLKEKMKEQSWNIHFSEYGRGTSMFFTRKTRDLIVRGVPEALRGELWMLFSGAVNDMATHPGYYTELVEQSLGTSTLATDEIERDLHRSLPEHPAFQSDTGISALRRVLTAYAYRNPKIGYCQAMNILTSVLLLYAKEEEAFWLLVAVCERMLPDYFNRRIIGALVDQAVFEDLIRENLPQLVEHMTDLSFFSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAILQLGLAVLDYNMEALISCHDDAEAVTILNKFFDSVTNKDSPLPPTVQQASVGNNDKSSHISVDISELIREAYEKYGNIRSEEVESSRKRNKLYVIQTLEDTTKQNVIRVVSQDVRFSASQLDELYNLFKRQHFLSCYWTMNSPALLHHDPSLAYLEQYQLGFQQFSMLFSLLEPWAFCSIKSTLSLWVFRLIDENQDGLINFKEFCCALDTLYTGSFTNKLKFLFKLHLPPAFDLPEDGVIRKSPERGRGKVDLQAYLKQWQNEILKKEETIKDLPRINQTQFIQFSKTLYNIFHGDPEEESLYRAVAHVTSLLLRMEEVGRRLQEPSSPPESKKPANTTAGEESSTEESSTTPESSDTSSSHNSQDLSETEWSFSFEQVLASLLNEPAIVSFFERPVDIQMKLGQAKVAQLKSKTNK

Target ORF information:

RefSeq Version	XM_005928488.1
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Definition	Haplochromis burtoni TBC1 domain family, member 8B (with GRAM domain) (tbc1d8b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005928488.1

ORF Insert Sequence:

ATGTGGCTCA AACCCGAAGA GATTCTGCTG AAAAATGCTT TTAAATTGTG GGTCACCGAG 
AAGGATAACG AGTATTTCGT GCTGCAGAGG AGACGAGGGT ACGGAGAAGG TGGTGGCGGT 
TTGACAGGCC TCCTTGTAGG AACCCTGGAT ACTGTGTTGG ACTCCACTTC CAAAGTGGCC 
CCTTATCGCA TTCTGCACCA CACGCCAGAC TCACAAGTGT ACTGGTCAAT AGCATGCGGT 
GTCACCAAAG AGGAGATTAT TCAGCACTGG GATTGGCTGC AGCAGAACAT TATGAGGACG 
CTCTCTGTTT TTGACTCCAG CGAAGACGTC ACCAGCTTTG TACAGGGCAA GATTAGAGGT 
CTTATTGCAG AAGAGGGGAA GACATGCTTA GTGCAGGAGG ATGATCCCGA GAAGTTTCGA 
GAAGCCCACT TGAGGTTTGA GAAGTGGTTT GAGCTGCCAC CAGAGGAAAA GCTGGTCACA 
TATTACTCAT GCAGCTACTG GAAAGGAAAA GTACCCTGCC AGGGCTGGCT CTACCTCAGC 
ACAAACTTCC TGTGCTTCTA TTCATACCTT CTGGGAGCTG AGGTTAAACT AGTCATCTCC 
TGGGATGAGC TTTGGAGGCT GGAGAAAACC TCTAATGTCA TTCTGACTGA GAGCATCCAT 
GTTTTGGCTC ATGGAGAGGA TCACTACTTC TCCATGCTGC TGCACCTGAA CGAAACGTTC 
GTTATCATGG AACAGTTGGC TGACTACTCC ATCAAACGTC TTTTTGATAA AGAGTCCTTC 
CAGAGAGAGC CAGCGCTGTC TGACCCGCTG CAGATTACAA AGAGAGGGCT AGAAGCTAAT 
GCAAAGAGTG AGCAATTCAG GACATTTTTC AGGCTTCCCA AAGAGGAAAA CCTGTTAGAG 
GTGCACGAGA GCTTTTTGTG GGTTCCCTTC AGCCATTTTA ACACGCTTGG CAAGATTTGT 
CTGTCGGAGA ACTACCTGTG TTTTGCCAGT CAGGATGGAA GTCAATGCCA TGTCATTATT 
CCAATGCGAG AGGTTGTTAA CGTTGAGAAA CCAGACTCCA GCAGCAGAGC TTTAACGGTG 
TGCGTGCGCG GCAAGAGGGC GCTGCGTTTC TCTGAGGTGC GGGACTATCA GCGGCTTGCC 
AATACAATCC GTAGTAGGTG TGGGATTAGT GCCAGCCCAC AGCACTCTGC TTCATCAGAG 
GCCATACGGG GAGAATGTCA GTCTCTCATA AACCACTTTG AGGACAATCC AGAGGACGTA 
ACTTTAATGG TGGGGCAGAA AGACAGCAGC AAGGCCGTGA GCACCGAGGC TCTCATGACT 
GTTTTCCACC CCCAGGATGT TGAAAACCTT GATCCCAAAA TGCTTAAGGA GAAGATGAAG 
GAGCAGTCTT GGAACATCCA TTTCTCTGAG TATGGACGTG GTACGAGTAT GTTCTTTACC 
AGGAAGACGC GAGACTTGAT TGTGCGCGGT GTTCCTGAGG CCTTGAGGGG AGAGCTGTGG 
ATGCTTTTCT CAGGGGCTGT AAACGACATG GCCACTCACC CCGGCTATTA CACTGAGCTG 
GTTGAACAGT CTCTGGGCAC AAGCACTCTG GCTACAGATG AGATTGAAAG AGACCTGCAC 
CGCTCTCTCC CTGAGCACCC GGCCTTTCAG AGCGACACAG GAATCTCAGC TCTTCGCAGA 
GTCCTTACTG CCTATGCCTA CAGGAACCCT AAAATTGGAT ACTGCCAGGC TATGAACATT 
CTCACCTCGG TTCTCCTGCT GTATGCAAAA GAAGAGGAGG CTTTCTGGCT GCTGGTAGCC 
GTCTGTGAGA GGATGCTGCC AGATTACTTC AACCGCCGAA TTATTGGTGC TTTGGTTGAC 
CAGGCTGTGT TCGAAGATCT GATTAGAGAA AACCTCCCCC AGCTGGTGGA ACACATGACA 
GACCTGAGCT TCTTCTCCTC CGTGTCTCTG TCCTGGTTTC TCACGCTTTT CATCAGTGTG 
CTGCCTATAG AGAGCGCCGT GAACGTGGTG GATTGTTTTT TTTATGATGG AATCAAAGCC 
ATCCTGCAGC TTGGCCTGGC CGTGCTGGAC TACAACATGG AGGCTCTGAT CAGCTGCCAC 
GACGATGCAG AAGCTGTCAC CATCCTCAAC AAGTTTTTTG ACAGTGTGAC AAACAAAGAC 
AGCCCATTGC CCCCGACAGT GCAGCAAGCT TCAGTAGGCA ATAATGATAA ATCATCTCAC 
ATCAGCGTAG ATATCAGTGA GCTGATCCGA GAGGCCTATG AGAAATATGG AAATATTCGT 
TCAGAGGAAG TTGAGAGTTC ACGGAAAAGA AACAAACTAT ATGTGATTCA AACACTGGAG 
GACACAACCA AGCAAAACGT GATCCGTGTG GTGTCCCAAG ATGTCAGATT CAGTGCCTCA 
CAACTGGACG AGCTTTATAA TTTATTCAAA AGGCAGCATT TCCTCAGCTG CTACTGGACA 
ATGAACAGCC CAGCTCTGCT CCATCATGAC CCCAGTCTGG CCTATCTGGA GCAGTACCAG 
CTGGGTTTCC AACAGTTCAG CATGCTCTTC TCCCTGCTGG AGCCCTGGGC TTTCTGCAGC 
ATCAAGAGCA CCCTCTCCCT CTGGGTTTTC CGCCTGATAG ATGAGAACCA GGATGGTCTC 
ATTAACTTTA AAGAATTCTG CTGTGCCCTT GATACTTTAT ACACCGGATC TTTCACAAAT 
AAGTTGAAAT TTCTCTTCAA GCTCCACCTG CCACCAGCCT TTGATCTTCC AGAAGATGGT 
GTGATAAGGA AAAGCCCTGA AAGAGGAAGA GGGAAGGTTG ACCTGCAGGC CTATCTGAAA 
CAATGGCAGA ATGAAATATT AAAGAAAGAG GAAACCATCA AAGACCTGCC CAGAATTAAT 
CAGACTCAGT TCATCCAGTT CTCCAAGACC CTGTACAACA TTTTTCATGG TGATCCCGAG 
GAGGAGTCAC TGTACCGAGC CGTGGCTCAT GTGACCAGCC TCCTCCTGAG GATGGAAGAG 
GTGGGGCGGA GGCTGCAGGA GCCGAGCAGC CCACCCGAGT CCAAAAAGCC TGCCAATACC 
ACTGCTGGCG AGGAGTCTTC AACTGAAGAG TCCTCCACAA CCCCAGAGAG CTCTGACACT 
TCCAGCTCCC ACAACAGTCA AGACCTCTCA GAGACCGAGT GGTCGTTTTC CTTCGAGCAG 
GTCCTAGCAT CTCTGCTCAA TGAACCGGCC ATAGTCAGCT TCTTTGAGAG GCCTGTTGAT 
ATTCAGATGA AGCTGGGACA AGCTAAGGTC GCCCAGCTGA AGTCAAAGAC CAATAAGTGA