ITSN1 cDNA ORF clone, Gallus gallus(chicken)

The following ITSN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITSN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa142936 XM_025145059.1
Latest version!
Gallus gallus intersectin 1 (ITSN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa142937 XM_025145063.1
Latest version!
Gallus gallus intersectin 1 (ITSN1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa30284 XM_015299993.2
Latest version!
Gallus gallus intersectin 1 (ITSN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa30285 XM_015299996.2
Latest version!
Gallus gallus intersectin 1 (ITSN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa30286 XM_015300003.2
Latest version!
Gallus gallus intersectin 1 (ITSN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa30287 XM_015300017.2
Latest version!
Gallus gallus intersectin 1 (ITSN1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa30288 XM_015300022.2
Latest version!
Gallus gallus intersectin 1 (ITSN1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa02710 XM_004934531.3
Latest version!
Gallus gallus intersectin 1 (ITSN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa02711 XM_416715.6
Latest version!
Gallus gallus intersectin 1 (ITSN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
OGa30287 XM_015300017.1 Gallus gallus intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa02711 XM_416715.5 Gallus gallus intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa30284 XM_015299993.1 Gallus gallus intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa30286 XM_015300003.1 Gallus gallus intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa30285 XM_015299996.1 Gallus gallus intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa02710 XM_004934531.2 Gallus gallus intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa30288 XM_015300022.1 Gallus gallus intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa142936
    Clone ID Related Accession (Same CDS sequence) XM_025145059.1
    Accession Version XM_025145059.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product intersectin-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGA AAAGCTCCAC TAACAATTTC TGCTCAGGAG
    GATGTAAAAA TAGTGTATTA TAGAGCACTG TATCCTTTTG AATCAAGAAG CCATGACGAA
    ATCACTATTC AACCTGGCGA CATAGTCATG GTGGATGAAA GTCAGACTGG AGAACCAGGG
    TGGCTTGGTG GAGAATTGAA GGGAAAAACT GGATGGTTCC CAGCAAACTA CGCAGAAAAA
    ATACCTGAAA GTGAAGTTCC AGCATCTATA AAACCAGTTG AGGCAGCTCC TGCACCTAAA
    GTTTCTGTGC ATGAAACAAC CACATCACTA GGAACTGCTG CATCTACAGA GTGCACGACA
    ACTGCTAACA ACTGGGCAGA CTTCAGTTCA ACATGGCCAG CAAACACTAA TGAGAAACCA
    GAGACAGACA ACTGGGATGC CTGGGCAGCT CAACCATCTT TGACTGTTCC CAGTGCAGGG
    CAGCTGCGGC AGCGATCAGC CTTCACCCCC GCCACTGTTA CAGGATCGTC TCCCTCTCCT
    GTATTGGGTC AGGGTGAAAA AGTGGAGGGA CTTCAAGCAC AGGCTCTGTA TCCTTGGAGA
    GCAAAAAAAG ACAACCACCT AAATTTCAAC AAAAATGATG TCATCACAGT TTTAGAGCAG
    CAAGATATGT GGTGGTTTGG AGAAGTTCAA GGACAAAAGG GGTGGTTTCC TAAGTCATAT
    GTGAAGCTTA TTTCAGGCCC CATTAGGAAG TCAACAAGCA TGGATTCTGG TTCTTCAGAA
    AGTCCTGCTA GTCTGAAAAG AGTAGCATCA CCAGCAGCTA AAGCAACTAT GTCAGGTGAA
    GAATATGTTG CTATGTATAC TTATGAGAGT TCTGAACAAG GAGACCTAAC CTTTCAGCAG
    GGTGATATGA TTCTTGTGAC AAAGAAAGAT GGCGACTGGT GGACAGGAAC ACTGGGTGAT
    AAAACAGGAG TTTTCCCATC AAATTATGTG AGACTCAAAG ATTCTGAGGC TTCTGGAGCA
    GCTGGAAAAA CAGGAAGCTT AGGGAAGAAA CCTGAAATTG CCCAAGTTAT TGCCTCTTAC
    ACAGCAACGG GTCCAGAACA ACTCACCCTT GCTCCTGGTC AGTTGATTCT TATCAGAAAG
    AAGAACCCAG GTGGCTGGTG GGAAGGAGAG CTCCAAGCAC GAGGGAAAAA ACGACAAATA
    GGATGGTTTC CTGCTAATTA TGTGAAACTT TTGAGCCCTG GTACCAGTAA AACTACACCA
    ACCGAGCTAC CTAAATCAAC AGCATTACCT TCAGTGTGCC AAGTAATTGG CATGTATGAC
    TACACTGCAC AGAATGATGA TGAGCTAGCA TTCAATAAAG GCCAGATTAT AAACGTCCTT
    AACAAGGAAG ACCCAGACTG GTGGAAAGGG GAGGTGAATG GACAAGTGGG TCTCTTCCCA
    TCCAACTATG TGAAGCTGAC AACAGATATG GACCCAAGCC AGCAATGGTG TGCAGACTTG
    CATCTTCTGG ATATGCTGAC ACCTACTGAA CGAAAAAGGC AGGGATACAT CCATGAGCTC
    ATTGTCACAG AAGAGAACTA CGTAAATGAT CTGCAGTTGG TCACAGAGAT CTTCCAGAAA
    CCACTAATGG AATCTGAGCT GCTAACAGAA AAGGAAGTTG CTATGATTTT TGTTAATTGG
    AAGGAGCTGA TTATGTGCAA TATAAAACTA CTGAAGGCCT TGCGTGTGCG TAAGAAGATG
    TCTGGGGAGA AGATGCCAGT GAAAATGATT GGTGACATCC TGACAGCTCA GCTGCCACAC
    ATGCAGCCAT ACATTCGGTT CTGTAGCTGC CAGCTCAATG GGGCAGCACT CATTCAGCAG
    AAAACAGATG AAGTCCCCGA ATTCAAGGAG TTTGTAAAAA GGTTAGCAAT GGATCCTCGC
    TGTAAAGGAA TGCCACTATC AAGTTTTCTA CTAAAGCCTA TGCAACGGGT AACAAGATAC
    CCACTAATAA TTAAAAATAT AATAGAAAAC ACTCCTGAAA ACCATCCTGA CCACAGTCAC
    TTGAAGCATG CTCTTGAAAA AGCAGAAGAA TTATGTTCTC AAGTAAATGA AGGAGTGCGA
    GAGAAAGAAA ATTCAGATAG GCTGGAGTGG ATCCAGGCTC ATGTTCAGTG CGAAGGCCTC
    TCTGAGCAAC TCGTATTTAA TTCTGTTACA AACTGCTTGG GACCACGCAA GTTTCTGCAC
    AGTGGGAAGC TCTATAAAGC CAAGAGTAAC AAGGAACTGT ATGGCTTTCT GTTCAACGAT
    TTCCTCCTCT TGACTCAGAT CATAAAACCT CTTGGCTCTT CTGGCACAGA CAAGGTCTTC
    AGCCCCAAGT CTAATCTGCA GTACAAAATG TACAAAACTC CCATTTTTCT AAATGAAGTA
    CTAGTAAAAT TACCCACAGA CCCTTCTGGA GATGAGCCCA TTTTCCATAT CTCCCACATT
    GACAGAGTCT ACACACTCCG GGCTGAAAGC ATTAATGAAA GGACTGCGTG GGTTCAGAAA
    ATTAAAGCTG CTTCGGAACT TTACATAGAG ACTGAAAAGA AGAAGAGGGA AAAGGCCTAC
    TTAGTTCGCT CACAAAGAGC AACAGGCATC GGGAGGTTGA TGGTGAATAT TGTTGAAGGC
    ATTGAACTAA AACCTTGCAG ATCCCATGGA AAGAGTAATC CATACTGTGA GGTGACGATG
    GGCTCTCAGT GCCACATCAC CAAGACAATG CAGGACACCC TCAATCCAAA GTGGAACTCC
    AACTGCCAGT TCTTTATCAA AGACCTCGAG CAGGACGTGC TCTGCATCAC GGTGTTTGAG
    AGGGACCAGT TCTCCCCAGA TGACTTTTTG GGCAGAACAG AGATCCGTGT GGCAGACATT
    AAGAAGGATC AGGGTTCAAA GGGACCAGTT ACTAAGTGTC TCCTTCTCCA TGAAGTCCCA
    ACAGGAGAGA TAGTCGTCCG ACTAGACCTG CAATTGTTTG ATGAGCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000827.1
    CDS231..5381
    Translation

    Target ORF information:

    RefSeq Version XM_025145059.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus intersectin 1 (ITSN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025145059.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGA AAAGCTCCAC TAACAATTTC TGCTCAGGAG
    GATGTAAAAA TAGTGTATTA TAGAGCACTG TATCCTTTTG AATCAAGAAG CCATGACGAA
    ATCACTATTC AACCTGGCGA CATAGTCATG GTGGATGAAA GTCAGACTGG AGAACCAGGG
    TGGCTTGGTG GAGAATTGAA GGGAAAAACT GGATGGTTCC CAGCAAACTA CGCAGAAAAA
    ATACCTGAAA GTGAAGTTCC AGCATCTATA AAACCAGTTG AGGCAGCTCC TGCACCTAAA
    GTTTCTGTGC ATGAAACAAC CACATCACTA GGAACTGCTG CATCTACAGA GTGCACGACA
    ACTGCTAACA ACTGGGCAGA CTTCAGTTCA ACATGGCCAG CAAACACTAA TGAGAAACCA
    GAGACAGACA ACTGGGATGC CTGGGCAGCT CAACCATCTT TGACTGTTCC CAGTGCAGGG
    CAGCTGCGGC AGCGATCAGC CTTCACCCCC GCCACTGTTA CAGGATCGTC TCCCTCTCCT
    GTATTGGGTC AGGGTGAAAA AGTGGAGGGA CTTCAAGCAC AGGCTCTGTA TCCTTGGAGA
    GCAAAAAAAG ACAACCACCT AAATTTCAAC AAAAATGATG TCATCACAGT TTTAGAGCAG
    CAAGATATGT GGTGGTTTGG AGAAGTTCAA GGACAAAAGG GGTGGTTTCC TAAGTCATAT
    GTGAAGCTTA TTTCAGGCCC CATTAGGAAG TCAACAAGCA TGGATTCTGG TTCTTCAGAA
    AGTCCTGCTA GTCTGAAAAG AGTAGCATCA CCAGCAGCTA AAGCAACTAT GTCAGGTGAA
    GAATATGTTG CTATGTATAC TTATGAGAGT TCTGAACAAG GAGACCTAAC CTTTCAGCAG
    GGTGATATGA TTCTTGTGAC AAAGAAAGAT GGCGACTGGT GGACAGGAAC ACTGGGTGAT
    AAAACAGGAG TTTTCCCATC AAATTATGTG AGACTCAAAG ATTCTGAGGC TTCTGGAGCA
    GCTGGAAAAA CAGGAAGCTT AGGGAAGAAA CCTGAAATTG CCCAAGTTAT TGCCTCTTAC
    ACAGCAACGG GTCCAGAACA ACTCACCCTT GCTCCTGGTC AGTTGATTCT TATCAGAAAG
    AAGAACCCAG GTGGCTGGTG GGAAGGAGAG CTCCAAGCAC GAGGGAAAAA ACGACAAATA
    GGATGGTTTC CTGCTAATTA TGTGAAACTT TTGAGCCCTG GTACCAGTAA AACTACACCA
    ACCGAGCTAC CTAAATCAAC AGCATTACCT TCAGTGTGCC AAGTAATTGG CATGTATGAC
    TACACTGCAC AGAATGATGA TGAGCTAGCA TTCAATAAAG GCCAGATTAT AAACGTCCTT
    AACAAGGAAG ACCCAGACTG GTGGAAAGGG GAGGTGAATG GACAAGTGGG TCTCTTCCCA
    TCCAACTATG TGAAGCTGAC AACAGATATG GACCCAAGCC AGCAATGGTG TGCAGACTTG
    CATCTTCTGG ATATGCTGAC ACCTACTGAA CGAAAAAGGC AGGGATACAT CCATGAGCTC
    ATTGTCACAG AAGAGAACTA CGTAAATGAT CTGCAGTTGG TCACAGAGAT CTTCCAGAAA
    CCACTAATGG AATCTGAGCT GCTAACAGAA AAGGAAGTTG CTATGATTTT TGTTAATTGG
    AAGGAGCTGA TTATGTGCAA TATAAAACTA CTGAAGGCCT TGCGTGTGCG TAAGAAGATG
    TCTGGGGAGA AGATGCCAGT GAAAATGATT GGTGACATCC TGACAGCTCA GCTGCCACAC
    ATGCAGCCAT ACATTCGGTT CTGTAGCTGC CAGCTCAATG GGGCAGCACT CATTCAGCAG
    AAAACAGATG AAGTCCCCGA ATTCAAGGAG TTTGTAAAAA GGTTAGCAAT GGATCCTCGC
    TGTAAAGGAA TGCCACTATC AAGTTTTCTA CTAAAGCCTA TGCAACGGGT AACAAGATAC
    CCACTAATAA TTAAAAATAT AATAGAAAAC ACTCCTGAAA ACCATCCTGA CCACAGTCAC
    TTGAAGCATG CTCTTGAAAA AGCAGAAGAA TTATGTTCTC AAGTAAATGA AGGAGTGCGA
    GAGAAAGAAA ATTCAGATAG GCTGGAGTGG ATCCAGGCTC ATGTTCAGTG CGAAGGCCTC
    TCTGAGCAAC TCGTATTTAA TTCTGTTACA AACTGCTTGG GACCACGCAA GTTTCTGCAC
    AGTGGGAAGC TCTATAAAGC CAAGAGTAAC AAGGAACTGT ATGGCTTTCT GTTCAACGAT
    TTCCTCCTCT TGACTCAGAT CATAAAACCT CTTGGCTCTT CTGGCACAGA CAAGGTCTTC
    AGCCCCAAGT CTAATCTGCA GTACAAAATG TACAAAACTC CCATTTTTCT AAATGAAGTA
    CTAGTAAAAT TACCCACAGA CCCTTCTGGA GATGAGCCCA TTTTCCATAT CTCCCACATT
    GACAGAGTCT ACACACTCCG GGCTGAAAGC ATTAATGAAA GGACTGCGTG GGTTCAGAAA
    ATTAAAGCTG CTTCGGAACT TTACATAGAG ACTGAAAAGA AGAAGAGGGA AAAGGCCTAC
    TTAGTTCGCT CACAAAGAGC AACAGGCATC GGGAGGTTGA TGGTGAATAT TGTTGAAGGC
    ATTGAACTAA AACCTTGCAG ATCCCATGGA AAGAGTAATC CATACTGTGA GGTGACGATG
    GGCTCTCAGT GCCACATCAC CAAGACAATG CAGGACACCC TCAATCCAAA GTGGAACTCC
    AACTGCCAGT TCTTTATCAA AGACCTCGAG CAGGACGTGC TCTGCATCAC GGTGTTTGAG
    AGGGACCAGT TCTCCCCAGA TGACTTTTTG GGCAGAACAG AGATCCGTGT GGCAGACATT
    AAGAAGGATC AGGGTTCAAA GGGACCAGTT ACTAAGTGTC TCCTTCTCCA TGAAGTCCCA
    ACAGGAGAGA TAGTCGTCCG ACTAGACCTG CAATTGTTTG ATGAGCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa142937
    Clone ID Related Accession (Same CDS sequence) XM_025145063.1
    Accession Version XM_025145063.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product intersectin-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GGAGCTGAGA GAAATACATA ACAGACAGCA GCTTCAGAAA
    CAAAAGAACT TGGAAGCTGA GAGGCTGAAA CAGAAAGAAC AAGAAAGGAA GACAGAACTG
    GAAAAGCAAA AAGAAGCTCA AAGGCGAATC CAGGATCGGG ATAAACAACG GCTTGATCGA
    GTGCAACAAG AAGAAGAACC TCAATGGCAA AAAAAAAATC AAGAAGATGA CAAGCAAAAA
    AGGGAAGAAA TAATCAAGAA GAAGGAAAGT GAGGATAAGG GGAAACAGGA AATCCAAGAG
    AAACCGAGTA AGCTTTTCCA ACCACATCAA GAACCAGTTA AGCCAGCTGT CCAGGCTCCA
    TGGTCAAATG CAGGAAAAGC TCCACTAACA ATTTCTGCTC AGGAGGATGT AAAAATAGTG
    TATTATAGAG CACTGTATCC TTTTGAATCA AGAAGCCATG ACGAAATCAC TATTCAACCT
    GGCGACATAG TCATGGTTAA AAGGGAATGG GTGGATGAAA GTCAGACTGG AGAACCAGGG
    TGGCTTGGTG GAGAATTGAA GGGAAAAACT GGATGGTTCC CAGCAAACTA CGCAGAAAAA
    ATACCTGAAA GTGAAGTTCC AGCATCTATA AAACCAGTTG AGGCAGCTCC TGCACCTAAA
    GTTTCTGTGC ATGAAACAAC CACATCACTA GGAACTGCTG CATCTACAGA GTGCACGACA
    ACTGCTAACA ACTGGGCAGA CTTCAGTTCA ACATGGCCAG CAAACACTAA TGAGAAACCA
    GAGACAGACA ACTGGGATGC CTGGGCAGCT CAACCATCTT TGACTGTTCC CAGTGCAGGG
    CAGCTGCGGC AGCGATCAGC CTTCACCCCC GCCACTGTTA CAGGATCGTC TCCCTCTCCT
    GTATTGGGTC AGGGTGAAAA AGTGGAGGGA CTTCAAGCAC AGGCTCTGTA TCCTTGGAGA
    GCAAAAAAAG ACAACCACCT AAATTTCAAC AAAAATGATG TCATCACAGT TTTAGAGCAG
    CAAGATATGT GGTGGTTTGG AGAAGTTCAA GGACAAAAGG GGTGGTTTCC TAAGTCATAT
    GTGAAGCTTA TTTCAGGCCC CATTAGGAAG TCAACAAGCA TGGATTCTGG TTCTTCAGAA
    AGTCCTGCTA GTCTGAAAAG AGTAGCATCA CCAGCAGCTA AAGCAACTAT GTCAGGTGAA
    GAATATGTTG CTATGTATAC TTATGAGAGT TCTGAACAAG GAGACCTAAC CTTTCAGCAG
    GGTGATATGA TTCTTGTGAC AAAGAAAGAT GGCGACTGGT GGACAGGAAC ACTGGGTGAT
    AAAACAGGAG TTTTCCCATC AAATTATGTG AGACTCAAAG ATTCTGAGGC TTCTGGAGCA
    GCTGGAAAAA CAGGAAGCTT AGGGAAGAAA CCTGAAATTG CCCAAGTTAT TGCCTCTTAC
    ACAGCAACGG GTCCAGAACA ACTCACCCTT GCTCCTGGTC AGTTGATTCT TATCAGAAAG
    AAGAACCCAG GTGGCTGGTG GGAAGGAGAG CTCCAAGCAC GAGGGAAAAA ACGACAAATA
    GGATGGTTTC CTGCTAATTA TGTGAAACTT TTGAGCCCTG GTACCAGTAA AACTACACCA
    ACCGAGCTAC CTAAATCAAC AGCATTACCT TCAGTGTGCC AAGTAATTGG CATGTATGAC
    TACACTGCAC AGAATGATGA TGAGCTAGCA TTCAATAAAG GCCAGATTAT AAACGTCCTT
    AACAAGGAAG ACCCAGACTG GTGGAAAGGG GAGGTGAATG GACAAGTGGG TCTCTTCCCA
    TCCAACTATG TGAAGCTGAC AACAGATATG GACCCAAGCC AGCAATGGTG TGCAGACTTG
    CATCTTCTGG ATATGCTGAC ACCTACTGAA CGAAAAAGGC AGGGATACAT CCATGAGCTC
    ATTGTCACAG AAGAGAACTA CGTAAATGAT CTGCAGTTGG TCACAGAGAT CTTCCAGAAA
    CCACTAATGG AATCTGAGCT GCTAACAGAA AAGGAAGTTG CTATGATTTT TGTTAATTGG
    AAGGAGCTGA TTATGTGCAA TATAAAACTA CTGAAGGCCT TGCGTGTGCG TAAGAAGATG
    TCTGGGGAGA AGATGCCAGT GAAAATGATT GGTGACATCC TGACAGCTCA GCTGCCACAC
    ATGCAGCCAT ACATTCGGTT CTGTAGCTGC CAGCTCAATG GGGCAGCACT CATTCAGCAG
    AAAACAGATG AAGTCCCCGA ATTCAAGGAG TTTGTAAAAA GGTTAGCAAT GGATCCTCGC
    TGTAAAGGAA TGCCACTATC AAGTTTTCTA CTAAAGCCTA TGCAACGGGT AACAAGATAC
    CCACTAATAA TTAAAAATAT AATAGAAAAC ACTCCTGAAA ACCATCCTGA CCACAGTCAC
    TTGAAGCATG CTCTTGAAAA AGCAGAAGAA TTATGTTCTC AAGTAAATGA AGGAGTGCGA
    GAGAAAGAAA ATTCAGATAG GCTGGAGTGG ATCCAGGCTC ATGTTCAGTG CGAAGGCCTC
    TCTGAGCAAC TCGTATTTAA TTCTGTTACA AACTGCTTGG GACCACGCAA GTTTCTGCAC
    AGTGGGAAGC TCTATAAAGC CAAGAGTAAC AAGGAACTGT ATGGCTTTCT GTTCAACGAT
    TTCCTCCTCT TGACTCAGAT CATAAAACCT CTTGGCTCTT CTGGCACAGA CAAGGTCTTC
    AGCCCCAAGT CTAATCTGCA GTACAAAATG TACAAAACTC CCATTTTTCT AAATGAAGTA
    CTAGTAAAAT TACCCACAGA CCCTTCTGGA GATGAGCCCA TTTTCCATAT CTCCCACATT
    GACAGAGTCT ACACACTCCG GGCTGAAAGC ATTAATGAAA GGACTGCGTG GGTTCAGAAA
    ATTAAAGCTG CTTCGGAACT TTACATAGAG ACTGAAAAGA AGAAGAGGGA AAAGGCCTAC
    TTAGTTCGCT CACAAAGAGC AACAGGCATC GGGAGGTTGA TGGTGAATAT TGTTGAAGGC
    ATTGAACTAA AACCTTGCAG ATCCCATGGA AAGAGTAATC CATACTGTGA GGTGACGATG
    GGCTCTCAGT GCCACATCAC CAAGACAATG CAGGACACCC TCAATCCAAA GTGGAACTCC
    AACTGCCAGT TCTTTATCAA AGACCTCGAG CAGGACGTGC TCTGCATCAC GGTGTTTGAG
    AGGGACCAGT TCTCCCCAGA TGACTTTTTG GGCAGAACAG AGATCCGTGT GGCAGACATT
    AAGAAGGATC AGGGTTCAAA GGGACCAGTT ACTAAGTGTC TCCTTCTCCA TGAAGTCCCA
    ACAGGAGAGA TAGTCGTCCG ACTAGACCTG CAATTGTTTG ATGAGCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000831.1
    CDS168..5138
    Translation

    Target ORF information:

    RefSeq Version XM_025145063.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus intersectin 1 (ITSN1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025145063.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GGAGCTGAGA GAAATACATA ACAGACAGCA GCTTCAGAAA
    CAAAAGAACT TGGAAGCTGA GAGGCTGAAA CAGAAAGAAC AAGAAAGGAA GACAGAACTG
    GAAAAGCAAA AAGAAGCTCA AAGGCGAATC CAGGATCGGG ATAAACAACG GCTTGATCGA
    GTGCAACAAG AAGAAGAACC TCAATGGCAA AAAAAAAATC AAGAAGATGA CAAGCAAAAA
    AGGGAAGAAA TAATCAAGAA GAAGGAAAGT GAGGATAAGG GGAAACAGGA AATCCAAGAG
    AAACCGAGTA AGCTTTTCCA ACCACATCAA GAACCAGTTA AGCCAGCTGT CCAGGCTCCA
    TGGTCAAATG CAGGAAAAGC TCCACTAACA ATTTCTGCTC AGGAGGATGT AAAAATAGTG
    TATTATAGAG CACTGTATCC TTTTGAATCA AGAAGCCATG ACGAAATCAC TATTCAACCT
    GGCGACATAG TCATGGTTAA AAGGGAATGG GTGGATGAAA GTCAGACTGG AGAACCAGGG
    TGGCTTGGTG GAGAATTGAA GGGAAAAACT GGATGGTTCC CAGCAAACTA CGCAGAAAAA
    ATACCTGAAA GTGAAGTTCC AGCATCTATA AAACCAGTTG AGGCAGCTCC TGCACCTAAA
    GTTTCTGTGC ATGAAACAAC CACATCACTA GGAACTGCTG CATCTACAGA GTGCACGACA
    ACTGCTAACA ACTGGGCAGA CTTCAGTTCA ACATGGCCAG CAAACACTAA TGAGAAACCA
    GAGACAGACA ACTGGGATGC CTGGGCAGCT CAACCATCTT TGACTGTTCC CAGTGCAGGG
    CAGCTGCGGC AGCGATCAGC CTTCACCCCC GCCACTGTTA CAGGATCGTC TCCCTCTCCT
    GTATTGGGTC AGGGTGAAAA AGTGGAGGGA CTTCAAGCAC AGGCTCTGTA TCCTTGGAGA
    GCAAAAAAAG ACAACCACCT AAATTTCAAC AAAAATGATG TCATCACAGT TTTAGAGCAG
    CAAGATATGT GGTGGTTTGG AGAAGTTCAA GGACAAAAGG GGTGGTTTCC TAAGTCATAT
    GTGAAGCTTA TTTCAGGCCC CATTAGGAAG TCAACAAGCA TGGATTCTGG TTCTTCAGAA
    AGTCCTGCTA GTCTGAAAAG AGTAGCATCA CCAGCAGCTA AAGCAACTAT GTCAGGTGAA
    GAATATGTTG CTATGTATAC TTATGAGAGT TCTGAACAAG GAGACCTAAC CTTTCAGCAG
    GGTGATATGA TTCTTGTGAC AAAGAAAGAT GGCGACTGGT GGACAGGAAC ACTGGGTGAT
    AAAACAGGAG TTTTCCCATC AAATTATGTG AGACTCAAAG ATTCTGAGGC TTCTGGAGCA
    GCTGGAAAAA CAGGAAGCTT AGGGAAGAAA CCTGAAATTG CCCAAGTTAT TGCCTCTTAC
    ACAGCAACGG GTCCAGAACA ACTCACCCTT GCTCCTGGTC AGTTGATTCT TATCAGAAAG
    AAGAACCCAG GTGGCTGGTG GGAAGGAGAG CTCCAAGCAC GAGGGAAAAA ACGACAAATA
    GGATGGTTTC CTGCTAATTA TGTGAAACTT TTGAGCCCTG GTACCAGTAA AACTACACCA
    ACCGAGCTAC CTAAATCAAC AGCATTACCT TCAGTGTGCC AAGTAATTGG CATGTATGAC
    TACACTGCAC AGAATGATGA TGAGCTAGCA TTCAATAAAG GCCAGATTAT AAACGTCCTT
    AACAAGGAAG ACCCAGACTG GTGGAAAGGG GAGGTGAATG GACAAGTGGG TCTCTTCCCA
    TCCAACTATG TGAAGCTGAC AACAGATATG GACCCAAGCC AGCAATGGTG TGCAGACTTG
    CATCTTCTGG ATATGCTGAC ACCTACTGAA CGAAAAAGGC AGGGATACAT CCATGAGCTC
    ATTGTCACAG AAGAGAACTA CGTAAATGAT CTGCAGTTGG TCACAGAGAT CTTCCAGAAA
    CCACTAATGG AATCTGAGCT GCTAACAGAA AAGGAAGTTG CTATGATTTT TGTTAATTGG
    AAGGAGCTGA TTATGTGCAA TATAAAACTA CTGAAGGCCT TGCGTGTGCG TAAGAAGATG
    TCTGGGGAGA AGATGCCAGT GAAAATGATT GGTGACATCC TGACAGCTCA GCTGCCACAC
    ATGCAGCCAT ACATTCGGTT CTGTAGCTGC CAGCTCAATG GGGCAGCACT CATTCAGCAG
    AAAACAGATG AAGTCCCCGA ATTCAAGGAG TTTGTAAAAA GGTTAGCAAT GGATCCTCGC
    TGTAAAGGAA TGCCACTATC AAGTTTTCTA CTAAAGCCTA TGCAACGGGT AACAAGATAC
    CCACTAATAA TTAAAAATAT AATAGAAAAC ACTCCTGAAA ACCATCCTGA CCACAGTCAC
    TTGAAGCATG CTCTTGAAAA AGCAGAAGAA TTATGTTCTC AAGTAAATGA AGGAGTGCGA
    GAGAAAGAAA ATTCAGATAG GCTGGAGTGG ATCCAGGCTC ATGTTCAGTG CGAAGGCCTC
    TCTGAGCAAC TCGTATTTAA TTCTGTTACA AACTGCTTGG GACCACGCAA GTTTCTGCAC
    AGTGGGAAGC TCTATAAAGC CAAGAGTAAC AAGGAACTGT ATGGCTTTCT GTTCAACGAT
    TTCCTCCTCT TGACTCAGAT CATAAAACCT CTTGGCTCTT CTGGCACAGA CAAGGTCTTC
    AGCCCCAAGT CTAATCTGCA GTACAAAATG TACAAAACTC CCATTTTTCT AAATGAAGTA
    CTAGTAAAAT TACCCACAGA CCCTTCTGGA GATGAGCCCA TTTTCCATAT CTCCCACATT
    GACAGAGTCT ACACACTCCG GGCTGAAAGC ATTAATGAAA GGACTGCGTG GGTTCAGAAA
    ATTAAAGCTG CTTCGGAACT TTACATAGAG ACTGAAAAGA AGAAGAGGGA AAAGGCCTAC
    TTAGTTCGCT CACAAAGAGC AACAGGCATC GGGAGGTTGA TGGTGAATAT TGTTGAAGGC
    ATTGAACTAA AACCTTGCAG ATCCCATGGA AAGAGTAATC CATACTGTGA GGTGACGATG
    GGCTCTCAGT GCCACATCAC CAAGACAATG CAGGACACCC TCAATCCAAA GTGGAACTCC
    AACTGCCAGT TCTTTATCAA AGACCTCGAG CAGGACGTGC TCTGCATCAC GGTGTTTGAG
    AGGGACCAGT TCTCCCCAGA TGACTTTTTG GGCAGAACAG AGATCCGTGT GGCAGACATT
    AAGAAGGATC AGGGTTCAAA GGGACCAGTT ACTAAGTGTC TCCTTCTCCA TGAAGTCCCA
    ACAGGAGAGA TAGTCGTCCG ACTAGACCTG CAATTGTTTG ATGAGCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30284
    Clone ID Related Accession (Same CDS sequence) XM_015299993.2 , XM_015299993.1
    Accession Version XM_015299993.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5232bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product intersectin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015299993.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGT AAGCTTTTCC AACCACATCA AGAACCAGTT
    AAGCCAGCTG TCCAGGCTCC ATGGTCAAAT GCAGGAAAAG CTCCACTAAC AATTTCTGCT
    CAGGAGGATG TAAAAATAGT GTATTATAGA GCACTGTATC CTTTTGAATC AAGAAGCCAT
    GACGAAATCA CTATTCAACC TGGCGACATA GTCATGGTTA AAAGGGAATG GGTGGATGAA
    AGTCAGACTG GAGAACCAGG GTGGCTTGGT GGAGAATTGA AGGGAAAAAC TGGATGGTTC
    CCAGCAAACT ACGCAGAAAA AATACCTGAA AGTGAAGTTC CAGCATCTAT AAAACCAGTT
    GAGGCAGCTC CTGCACCTAA AGTTTCTGTG CATGAAACAA CCACATCACT AGGAACTGCT
    GCATCTACAG AGTGCACGAC AACTGCTAAC AACTGGGCAG ACTTCAGTTC AACATGGCCA
    GCAAACACTA ATGAGAAACC AGAGACAGAC AACTGGGATG CCTGGGCAGC TCAACCATCT
    TTGACTGTTC CCAGTGCAGG GCAGCTGCGG CAGCGATCAG CCTTCACCCC CGCCACTGTT
    ACAGGATCGT CTCCCTCTCC TGTATTGGGT CAGGGTGAAA AAGTGGAGGG ACTTCAAGCA
    CAGGCTCTGT ATCCTTGGAG AGCAAAAAAA GACAACCACC TAAATTTCAA CAAAAATGAT
    GTCATCACAG TTTTAGAGCA GCAAGATATG TGGTGGTTTG GAGAAGTTCA AGGACAAAAG
    GGGTGGTTTC CTAAGTCATA TGTGAAGCTT ATTTCAGGCC CCATTAGGAA GTCAACAAGC
    ATGGATTCTG GTTCTTCAGA AAGTCCTGCT AGTCTGAAAA GAGTAGCATC ACCAGCAGCT
    AAAGCAACTA TGTCAGGTGA AGAATATGTT GCTATGTATA CTTATGAGAG TTCTGAACAA
    GGAGACCTAA CCTTTCAGCA GGGTGATATG ATTCTTGTGA CAAAGAAAGA TGGCGACTGG
    TGGACAGGAA CACTGGGTGA TAAAACAGGA GTTTTCCCAT CAAATTATGT GAGACTCAAA
    GATTCTGAGG CTTCTGGAGC AGCTGGAAAA ACAGGAAGCT TAGGGAAGAA ACCTGAAATT
    GCCCAAGTTA TTGCCTCTTA CACAGCAACG GGTCCAGAAC AACTCACCCT TGCTCCTGGT
    CAGTTGATTC TTATCAGAAA GAAGAACCCA GGTGGCTGGT GGGAAGGAGA GCTCCAAGCA
    CGAGGGAAAA AACGACAAAT AGGATGGTTT CCTGCTAATT ATGTGAAACT TTTGAGCCCT
    GGTACCAGTA AAACTACACC AACCGAGCTA CCTAAATCAA CAGCATTACC TTCAGTGTGC
    CAAGTAATTG GCATGTATGA CTACACTGCA CAGAATGATG ATGAGCTAGC ATTCAATAAA
    GGCCAGATTA TAAACGTCCT TAACAAGGAA GACCCAGACT GGTGGAAAGG GGAGGTGAAT
    GGACAAGTGG GTCTCTTCCC ATCCAACTAT GTGAAGCTGA CAACAGATAT GGACCCAAGC
    CAGCAATGGT GTGCAGACTT GCATCTTCTG GATATGCTGA CACCTACTGA ACGAAAAAGG
    CAGGGATACA TCCATGAGCT CATTGTCACA GAAGAGAACT ACGTAAATGA TCTGCAGTTG
    GTCACAGAGA TCTTCCAGAA ACCACTAATG GAATCTGAGC TGCTAACAGA AAAGGAAGTT
    GCTATGATTT TTGTTAATTG GAAGGAGCTG ATTATGTGCA ATATAAAACT ACTGAAGGCC
    TTGCGTGTGC GTAAGAAGAT GTCTGGGGAG AAGATGCCAG TGAAAATGAT TGGTGACATC
    CTGACAGCTC AGCTGCCACA CATGCAGCCA TACATTCGGT TCTGTAGCTG CCAGCTCAAT
    GGGGCAGCAC TCATTCAGCA GAAAACAGAT GAAGTCCCCG AATTCAAGGA GTTTGTAAAA
    AGGTTAGCAA TGGATCCTCG CTGTAAAGGA ATGCCACTAT CAAGTTTTCT ACTAAAGCCT
    ATGCAACGGG TAACAAGATA CCCACTAATA ATTAAAAATA TAATAGAAAA CACTCCTGAA
    AACCATCCTG ACCACAGTCA CTTGAAGCAT GCTCTTGAAA AAGCAGAAGA ATTATGTTCT
    CAAGTAAATG AAGGAGTGCG AGAGAAAGAA AATTCAGATA GGCTGGAGTG GATCCAGGCT
    CATGTTCAGT GCGAAGGCCT CTCTGAGCAA CTCGTATTTA ATTCTGTTAC AAACTGCTTG
    GGACCACGCA AGTTTCTGCA CAGTGGGAAG CTCTATAAAG CCAAGAGTAA CAAGGAACTG
    TATGGCTTTC TGTTCAACGA TTTCCTCCTC TTGACTCAGA TCATAAAACC TCTTGGCTCT
    TCTGGCACAG ACAAGGTCTT CAGCCCCAAG TCTAATCTGC AGTACAAAAT GTACAAAACT
    CCCATTTTTC TAAATGAAGT ACTAGTAAAA TTACCCACAG ACCCTTCTGG AGATGAGCCC
    ATTTTCCATA TCTCCCACAT TGACAGAGTC TACACACTCC GGGCTGAAAG CATTAATGAA
    AGGACTGCGT GGGTTCAGAA AATTAAAGCT GCTTCGGAAC TTTACATAGA GACTGAAAAG
    AAGAAGAGGG AAAAGGCCTA CTTAGTTCGC TCACAAAGAG CAACAGGCAT CGGGAGGTTG
    ATGGTGAATA TTGTTGAAGG CATTGAACTA AAACCTTGCA GATCCCATGG AAAGAGTAAT
    CCATACTGTG AGGTGACGAT GGGCTCTCAG TGCCACATCA CCAAGACAAT GCAGGACACC
    CTCAATCCAA AGTGGAACTC CAACTGCCAG TTCTTTATCA AAGACCTCGA GCAGGACGTG
    CTCTGCATCA CGGTGTTTGA GAGGGACCAG TTCTCCCCAG ATGACTTTTT GGGCAGAACA
    GAGATCCGTG TGGCAGACAT TAAGAAGGAT CAGGGTTCAA AGGGACCAGT TACTAAGTGT
    CTCCTTCTCC ATGAAGTCCC AACAGGAGAG ATAGTCGTCC GACTAGACCT GCAATTGTTT
    GATGAGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015155479.1
    CDS232..5463
    Translation

    Target ORF information:

    RefSeq Version XM_015299993.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus intersectin 1 (ITSN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015299993.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGT AAGCTTTTCC AACCACATCA AGAACCAGTT
    AAGCCAGCTG TCCAGGCTCC ATGGTCAAAT GCAGGAAAAG CTCCACTAAC AATTTCTGCT
    CAGGAGGATG TAAAAATAGT GTATTATAGA GCACTGTATC CTTTTGAATC AAGAAGCCAT
    GACGAAATCA CTATTCAACC TGGCGACATA GTCATGGTTA AAAGGGAATG GGTGGATGAA
    AGTCAGACTG GAGAACCAGG GTGGCTTGGT GGAGAATTGA AGGGAAAAAC TGGATGGTTC
    CCAGCAAACT ACGCAGAAAA AATACCTGAA AGTGAAGTTC CAGCATCTAT AAAACCAGTT
    GAGGCAGCTC CTGCACCTAA AGTTTCTGTG CATGAAACAA CCACATCACT AGGAACTGCT
    GCATCTACAG AGTGCACGAC AACTGCTAAC AACTGGGCAG ACTTCAGTTC AACATGGCCA
    GCAAACACTA ATGAGAAACC AGAGACAGAC AACTGGGATG CCTGGGCAGC TCAACCATCT
    TTGACTGTTC CCAGTGCAGG GCAGCTGCGG CAGCGATCAG CCTTCACCCC CGCCACTGTT
    ACAGGATCGT CTCCCTCTCC TGTATTGGGT CAGGGTGAAA AAGTGGAGGG ACTTCAAGCA
    CAGGCTCTGT ATCCTTGGAG AGCAAAAAAA GACAACCACC TAAATTTCAA CAAAAATGAT
    GTCATCACAG TTTTAGAGCA GCAAGATATG TGGTGGTTTG GAGAAGTTCA AGGACAAAAG
    GGGTGGTTTC CTAAGTCATA TGTGAAGCTT ATTTCAGGCC CCATTAGGAA GTCAACAAGC
    ATGGATTCTG GTTCTTCAGA AAGTCCTGCT AGTCTGAAAA GAGTAGCATC ACCAGCAGCT
    AAAGCAACTA TGTCAGGTGA AGAATATGTT GCTATGTATA CTTATGAGAG TTCTGAACAA
    GGAGACCTAA CCTTTCAGCA GGGTGATATG ATTCTTGTGA CAAAGAAAGA TGGCGACTGG
    TGGACAGGAA CACTGGGTGA TAAAACAGGA GTTTTCCCAT CAAATTATGT GAGACTCAAA
    GATTCTGAGG CTTCTGGAGC AGCTGGAAAA ACAGGAAGCT TAGGGAAGAA ACCTGAAATT
    GCCCAAGTTA TTGCCTCTTA CACAGCAACG GGTCCAGAAC AACTCACCCT TGCTCCTGGT
    CAGTTGATTC TTATCAGAAA GAAGAACCCA GGTGGCTGGT GGGAAGGAGA GCTCCAAGCA
    CGAGGGAAAA AACGACAAAT AGGATGGTTT CCTGCTAATT ATGTGAAACT TTTGAGCCCT
    GGTACCAGTA AAACTACACC AACCGAGCTA CCTAAATCAA CAGCATTACC TTCAGTGTGC
    CAAGTAATTG GCATGTATGA CTACACTGCA CAGAATGATG ATGAGCTAGC ATTCAATAAA
    GGCCAGATTA TAAACGTCCT TAACAAGGAA GACCCAGACT GGTGGAAAGG GGAGGTGAAT
    GGACAAGTGG GTCTCTTCCC ATCCAACTAT GTGAAGCTGA CAACAGATAT GGACCCAAGC
    CAGCAATGGT GTGCAGACTT GCATCTTCTG GATATGCTGA CACCTACTGA ACGAAAAAGG
    CAGGGATACA TCCATGAGCT CATTGTCACA GAAGAGAACT ACGTAAATGA TCTGCAGTTG
    GTCACAGAGA TCTTCCAGAA ACCACTAATG GAATCTGAGC TGCTAACAGA AAAGGAAGTT
    GCTATGATTT TTGTTAATTG GAAGGAGCTG ATTATGTGCA ATATAAAACT ACTGAAGGCC
    TTGCGTGTGC GTAAGAAGAT GTCTGGGGAG AAGATGCCAG TGAAAATGAT TGGTGACATC
    CTGACAGCTC AGCTGCCACA CATGCAGCCA TACATTCGGT TCTGTAGCTG CCAGCTCAAT
    GGGGCAGCAC TCATTCAGCA GAAAACAGAT GAAGTCCCCG AATTCAAGGA GTTTGTAAAA
    AGGTTAGCAA TGGATCCTCG CTGTAAAGGA ATGCCACTAT CAAGTTTTCT ACTAAAGCCT
    ATGCAACGGG TAACAAGATA CCCACTAATA ATTAAAAATA TAATAGAAAA CACTCCTGAA
    AACCATCCTG ACCACAGTCA CTTGAAGCAT GCTCTTGAAA AAGCAGAAGA ATTATGTTCT
    CAAGTAAATG AAGGAGTGCG AGAGAAAGAA AATTCAGATA GGCTGGAGTG GATCCAGGCT
    CATGTTCAGT GCGAAGGCCT CTCTGAGCAA CTCGTATTTA ATTCTGTTAC AAACTGCTTG
    GGACCACGCA AGTTTCTGCA CAGTGGGAAG CTCTATAAAG CCAAGAGTAA CAAGGAACTG
    TATGGCTTTC TGTTCAACGA TTTCCTCCTC TTGACTCAGA TCATAAAACC TCTTGGCTCT
    TCTGGCACAG ACAAGGTCTT CAGCCCCAAG TCTAATCTGC AGTACAAAAT GTACAAAACT
    CCCATTTTTC TAAATGAAGT ACTAGTAAAA TTACCCACAG ACCCTTCTGG AGATGAGCCC
    ATTTTCCATA TCTCCCACAT TGACAGAGTC TACACACTCC GGGCTGAAAG CATTAATGAA
    AGGACTGCGT GGGTTCAGAA AATTAAAGCT GCTTCGGAAC TTTACATAGA GACTGAAAAG
    AAGAAGAGGG AAAAGGCCTA CTTAGTTCGC TCACAAAGAG CAACAGGCAT CGGGAGGTTG
    ATGGTGAATA TTGTTGAAGG CATTGAACTA AAACCTTGCA GATCCCATGG AAAGAGTAAT
    CCATACTGTG AGGTGACGAT GGGCTCTCAG TGCCACATCA CCAAGACAAT GCAGGACACC
    CTCAATCCAA AGTGGAACTC CAACTGCCAG TTCTTTATCA AAGACCTCGA GCAGGACGTG
    CTCTGCATCA CGGTGTTTGA GAGGGACCAG TTCTCCCCAG ATGACTTTTT GGGCAGAACA
    GAGATCCGTG TGGCAGACAT TAAGAAGGAT CAGGGTTCAA AGGGACCAGT TACTAAGTGT
    CTCCTTCTCC ATGAAGTCCC AACAGGAGAG ATAGTCGTCC GACTAGACCT GCAATTGTTT
    GATGAGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30284
    Clone ID Related Accession (Same CDS sequence) XM_015299993.2 , XM_015299993.1
    Accession Version XM_015299993.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5232bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product intersectin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455737.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGT AAGCTTTTCC AACCACATCA AGAACCAGTT
    AAGCCAGCTG TCCAGGCTCC ATGGTCAAAT GCAGGAAAAG CTCCACTAAC AATTTCTGCT
    CAGGAGGATG TAAAAATAGT GTATTATAGA GCACTGTATC CTTTTGAATC AAGAAGCCAT
    GACGAAATCA CTATTCAACC TGGCGACATA GTCATGGTTA AAAGGGAATG GGTGGATGAA
    AGTCAGACTG GAGAACCAGG GTGGCTTGGT GGAGAATTGA AGGGAAAAAC TGGATGGTTC
    CCAGCAAACT ACGCAGAAAA AATACCTGAA AGTGAAGTTC CAGCATCTAT AAAACCAGTT
    GAGGCAGCTC CTGCACCTAA AGTTTCTGTG CATGAAACAA CCACATCACT AGGAACTGCT
    GCATCTACAG AGTGCACGAC AACTGCTAAC AACTGGGCAG ACTTCAGTTC AACATGGCCA
    GCAAACACTA ATGAGAAACC AGAGACAGAC AACTGGGATG CCTGGGCAGC TCAACCATCT
    TTGACTGTTC CCAGTGCAGG GCAGCTGCGG CAGCGATCAG CCTTCACCCC CGCCACTGTT
    ACAGGATCGT CTCCCTCTCC TGTATTGGGT CAGGGTGAAA AAGTGGAGGG ACTTCAAGCA
    CAGGCTCTGT ATCCTTGGAG AGCAAAAAAA GACAACCACC TAAATTTCAA CAAAAATGAT
    GTCATCACAG TTTTAGAGCA GCAAGATATG TGGTGGTTTG GAGAAGTTCA AGGACAAAAG
    GGGTGGTTTC CTAAGTCATA TGTGAAGCTT ATTTCAGGCC CCATTAGGAA GTCAACAAGC
    ATGGATTCTG GTTCTTCAGA AAGTCCTGCT AGTCTGAAAA GAGTAGCATC ACCAGCAGCT
    AAAGCAACTA TGTCAGGTGA AGAATATGTT GCTATGTATA CTTATGAGAG TTCTGAACAA
    GGAGACCTAA CCTTTCAGCA GGGTGATATG ATTCTTGTGA CAAAGAAAGA TGGCGACTGG
    TGGACAGGAA CACTGGGTGA TAAAACAGGA GTTTTCCCAT CAAATTATGT GAGACTCAAA
    GATTCTGAGG CTTCTGGAGC AGCTGGAAAA ACAGGAAGCT TAGGGAAGAA ACCTGAAATT
    GCCCAAGTTA TTGCCTCTTA CACAGCAACG GGTCCAGAAC AACTCACCCT TGCTCCTGGT
    CAGTTGATTC TTATCAGAAA GAAGAACCCA GGTGGCTGGT GGGAAGGAGA GCTCCAAGCA
    CGAGGGAAAA AACGACAAAT AGGATGGTTT CCTGCTAATT ATGTGAAACT TTTGAGCCCT
    GGTACCAGTA AAACTACACC AACCGAGCTA CCTAAATCAA CAGCATTACC TTCAGTGTGC
    CAAGTAATTG GCATGTATGA CTACACTGCA CAGAATGATG ATGAGCTAGC ATTCAATAAA
    GGCCAGATTA TAAACGTCCT TAACAAGGAA GACCCAGACT GGTGGAAAGG GGAGGTGAAT
    GGACAAGTGG GTCTCTTCCC ATCCAACTAT GTGAAGCTGA CAACAGATAT GGACCCAAGC
    CAGCAATGGT GTGCAGACTT GCATCTTCTG GATATGCTGA CACCTACTGA ACGAAAAAGG
    CAGGGATACA TCCATGAGCT CATTGTCACA GAAGAGAACT ACGTAAATGA TCTGCAGTTG
    GTCACAGAGA TCTTCCAGAA ACCACTAATG GAATCTGAGC TGCTAACAGA AAAGGAAGTT
    GCTATGATTT TTGTTAATTG GAAGGAGCTG ATTATGTGCA ATATAAAACT ACTGAAGGCC
    TTGCGTGTGC GTAAGAAGAT GTCTGGGGAG AAGATGCCAG TGAAAATGAT TGGTGACATC
    CTGACAGCTC AGCTGCCACA CATGCAGCCA TACATTCGGT TCTGTAGCTG CCAGCTCAAT
    GGGGCAGCAC TCATTCAGCA GAAAACAGAT GAAGTCCCCG AATTCAAGGA GTTTGTAAAA
    AGGTTAGCAA TGGATCCTCG CTGTAAAGGA ATGCCACTAT CAAGTTTTCT ACTAAAGCCT
    ATGCAACGGG TAACAAGATA CCCACTAATA ATTAAAAATA TAATAGAAAA CACTCCTGAA
    AACCATCCTG ACCACAGTCA CTTGAAGCAT GCTCTTGAAA AAGCAGAAGA ATTATGTTCT
    CAAGTAAATG AAGGAGTGCG AGAGAAAGAA AATTCAGATA GGCTGGAGTG GATCCAGGCT
    CATGTTCAGT GCGAAGGCCT CTCTGAGCAA CTCGTATTTA ATTCTGTTAC AAACTGCTTG
    GGACCACGCA AGTTTCTGCA CAGTGGGAAG CTCTATAAAG CCAAGAGTAA CAAGGAACTG
    TATGGCTTTC TGTTCAACGA TTTCCTCCTC TTGACTCAGA TCATAAAACC TCTTGGCTCT
    TCTGGCACAG ACAAGGTCTT CAGCCCCAAG TCTAATCTGC AGTACAAAAT GTACAAAACT
    CCCATTTTTC TAAATGAAGT ACTAGTAAAA TTACCCACAG ACCCTTCTGG AGATGAGCCC
    ATTTTCCATA TCTCCCACAT TGACAGAGTC TACACACTCC GGGCTGAAAG CATTAATGAA
    AGGACTGCGT GGGTTCAGAA AATTAAAGCT GCTTCGGAAC TTTACATAGA GACTGAAAAG
    AAGAAGAGGG AAAAGGCCTA CTTAGTTCGC TCACAAAGAG CAACAGGCAT CGGGAGGTTG
    ATGGTGAATA TTGTTGAAGG CATTGAACTA AAACCTTGCA GATCCCATGG AAAGAGTAAT
    CCATACTGTG AGGTGACGAT GGGCTCTCAG TGCCACATCA CCAAGACAAT GCAGGACACC
    CTCAATCCAA AGTGGAACTC CAACTGCCAG TTCTTTATCA AAGACCTCGA GCAGGACGTG
    CTCTGCATCA CGGTGTTTGA GAGGGACCAG TTCTCCCCAG ATGACTTTTT GGGCAGAACA
    GAGATCCGTG TGGCAGACAT TAAGAAGGAT CAGGGTTCAA AGGGACCAGT TACTAAGTGT
    CTCCTTCTCC ATGAAGTCCC AACAGGAGAG ATAGTCGTCC GACTAGACCT GCAATTGTTT
    GATGAGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015155479.1
    CDS213..5444
    Translation

    Target ORF information:

    RefSeq Version XM_015299993.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015299993.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGT AAGCTTTTCC AACCACATCA AGAACCAGTT
    AAGCCAGCTG TCCAGGCTCC ATGGTCAAAT GCAGGAAAAG CTCCACTAAC AATTTCTGCT
    CAGGAGGATG TAAAAATAGT GTATTATAGA GCACTGTATC CTTTTGAATC AAGAAGCCAT
    GACGAAATCA CTATTCAACC TGGCGACATA GTCATGGTTA AAAGGGAATG GGTGGATGAA
    AGTCAGACTG GAGAACCAGG GTGGCTTGGT GGAGAATTGA AGGGAAAAAC TGGATGGTTC
    CCAGCAAACT ACGCAGAAAA AATACCTGAA AGTGAAGTTC CAGCATCTAT AAAACCAGTT
    GAGGCAGCTC CTGCACCTAA AGTTTCTGTG CATGAAACAA CCACATCACT AGGAACTGCT
    GCATCTACAG AGTGCACGAC AACTGCTAAC AACTGGGCAG ACTTCAGTTC AACATGGCCA
    GCAAACACTA ATGAGAAACC AGAGACAGAC AACTGGGATG CCTGGGCAGC TCAACCATCT
    TTGACTGTTC CCAGTGCAGG GCAGCTGCGG CAGCGATCAG CCTTCACCCC CGCCACTGTT
    ACAGGATCGT CTCCCTCTCC TGTATTGGGT CAGGGTGAAA AAGTGGAGGG ACTTCAAGCA
    CAGGCTCTGT ATCCTTGGAG AGCAAAAAAA GACAACCACC TAAATTTCAA CAAAAATGAT
    GTCATCACAG TTTTAGAGCA GCAAGATATG TGGTGGTTTG GAGAAGTTCA AGGACAAAAG
    GGGTGGTTTC CTAAGTCATA TGTGAAGCTT ATTTCAGGCC CCATTAGGAA GTCAACAAGC
    ATGGATTCTG GTTCTTCAGA AAGTCCTGCT AGTCTGAAAA GAGTAGCATC ACCAGCAGCT
    AAAGCAACTA TGTCAGGTGA AGAATATGTT GCTATGTATA CTTATGAGAG TTCTGAACAA
    GGAGACCTAA CCTTTCAGCA GGGTGATATG ATTCTTGTGA CAAAGAAAGA TGGCGACTGG
    TGGACAGGAA CACTGGGTGA TAAAACAGGA GTTTTCCCAT CAAATTATGT GAGACTCAAA
    GATTCTGAGG CTTCTGGAGC AGCTGGAAAA ACAGGAAGCT TAGGGAAGAA ACCTGAAATT
    GCCCAAGTTA TTGCCTCTTA CACAGCAACG GGTCCAGAAC AACTCACCCT TGCTCCTGGT
    CAGTTGATTC TTATCAGAAA GAAGAACCCA GGTGGCTGGT GGGAAGGAGA GCTCCAAGCA
    CGAGGGAAAA AACGACAAAT AGGATGGTTT CCTGCTAATT ATGTGAAACT TTTGAGCCCT
    GGTACCAGTA AAACTACACC AACCGAGCTA CCTAAATCAA CAGCATTACC TTCAGTGTGC
    CAAGTAATTG GCATGTATGA CTACACTGCA CAGAATGATG ATGAGCTAGC ATTCAATAAA
    GGCCAGATTA TAAACGTCCT TAACAAGGAA GACCCAGACT GGTGGAAAGG GGAGGTGAAT
    GGACAAGTGG GTCTCTTCCC ATCCAACTAT GTGAAGCTGA CAACAGATAT GGACCCAAGC
    CAGCAATGGT GTGCAGACTT GCATCTTCTG GATATGCTGA CACCTACTGA ACGAAAAAGG
    CAGGGATACA TCCATGAGCT CATTGTCACA GAAGAGAACT ACGTAAATGA TCTGCAGTTG
    GTCACAGAGA TCTTCCAGAA ACCACTAATG GAATCTGAGC TGCTAACAGA AAAGGAAGTT
    GCTATGATTT TTGTTAATTG GAAGGAGCTG ATTATGTGCA ATATAAAACT ACTGAAGGCC
    TTGCGTGTGC GTAAGAAGAT GTCTGGGGAG AAGATGCCAG TGAAAATGAT TGGTGACATC
    CTGACAGCTC AGCTGCCACA CATGCAGCCA TACATTCGGT TCTGTAGCTG CCAGCTCAAT
    GGGGCAGCAC TCATTCAGCA GAAAACAGAT GAAGTCCCCG AATTCAAGGA GTTTGTAAAA
    AGGTTAGCAA TGGATCCTCG CTGTAAAGGA ATGCCACTAT CAAGTTTTCT ACTAAAGCCT
    ATGCAACGGG TAACAAGATA CCCACTAATA ATTAAAAATA TAATAGAAAA CACTCCTGAA
    AACCATCCTG ACCACAGTCA CTTGAAGCAT GCTCTTGAAA AAGCAGAAGA ATTATGTTCT
    CAAGTAAATG AAGGAGTGCG AGAGAAAGAA AATTCAGATA GGCTGGAGTG GATCCAGGCT
    CATGTTCAGT GCGAAGGCCT CTCTGAGCAA CTCGTATTTA ATTCTGTTAC AAACTGCTTG
    GGACCACGCA AGTTTCTGCA CAGTGGGAAG CTCTATAAAG CCAAGAGTAA CAAGGAACTG
    TATGGCTTTC TGTTCAACGA TTTCCTCCTC TTGACTCAGA TCATAAAACC TCTTGGCTCT
    TCTGGCACAG ACAAGGTCTT CAGCCCCAAG TCTAATCTGC AGTACAAAAT GTACAAAACT
    CCCATTTTTC TAAATGAAGT ACTAGTAAAA TTACCCACAG ACCCTTCTGG AGATGAGCCC
    ATTTTCCATA TCTCCCACAT TGACAGAGTC TACACACTCC GGGCTGAAAG CATTAATGAA
    AGGACTGCGT GGGTTCAGAA AATTAAAGCT GCTTCGGAAC TTTACATAGA GACTGAAAAG
    AAGAAGAGGG AAAAGGCCTA CTTAGTTCGC TCACAAAGAG CAACAGGCAT CGGGAGGTTG
    ATGGTGAATA TTGTTGAAGG CATTGAACTA AAACCTTGCA GATCCCATGG AAAGAGTAAT
    CCATACTGTG AGGTGACGAT GGGCTCTCAG TGCCACATCA CCAAGACAAT GCAGGACACC
    CTCAATCCAA AGTGGAACTC CAACTGCCAG TTCTTTATCA AAGACCTCGA GCAGGACGTG
    CTCTGCATCA CGGTGTTTGA GAGGGACCAG TTCTCCCCAG ATGACTTTTT GGGCAGAACA
    GAGATCCGTG TGGCAGACAT TAAGAAGGAT CAGGGTTCAA AGGGACCAGT TACTAAGTGT
    CTCCTTCTCC ATGAAGTCCC AACAGGAGAG ATAGTCGTCC GACTAGACCT GCAATTGTTT
    GATGAGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30285
    Clone ID Related Accession (Same CDS sequence) XM_015299996.1 , XM_015299996.2
    Accession Version XM_015299996.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product intersectin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455737.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGT AAGCTTTTCC AACCACATCA AGAACCAGTT
    AAGCCAGCTG TCCAGGCTCC ATGGTCAAAT GCAGGAAAAG CTCCACTAAC AATTTCTGCT
    CAGGAGGATG TAAAAATAGT GTATTATAGA GCACTGTATC CTTTTGAATC AAGAAGCCAT
    GACGAAATCA CTATTCAACC TGGCGACATA GTCATGGTGG ATGAAAGTCA GACTGGAGAA
    CCAGGGTGGC TTGGTGGAGA ATTGAAGGGA AAAACTGGAT GGTTCCCAGC AAACTACGCA
    GAAAAAATAC CTGAAAGTGA AGTTCCAGCA TCTATAAAAC CAGTTGAGGC AGCTCCTGCA
    CCTAAAGTTT CTGTGCATGA AACAACCACA TCACTAGGAA CTGCTGCATC TACAGAGTGC
    ACGACAACTG CTAACAACTG GGCAGACTTC AGTTCAACAT GGCCAGCAAA CACTAATGAG
    AAACCAGAGA CAGACAACTG GGATGCCTGG GCAGCTCAAC CATCTTTGAC TGTTCCCAGT
    GCAGGGCAGC TGCGGCAGCG ATCAGCCTTC ACCCCCGCCA CTGTTACAGG ATCGTCTCCC
    TCTCCTGTAT TGGGTCAGGG TGAAAAAGTG GAGGGACTTC AAGCACAGGC TCTGTATCCT
    TGGAGAGCAA AAAAAGACAA CCACCTAAAT TTCAACAAAA ATGATGTCAT CACAGTTTTA
    GAGCAGCAAG ATATGTGGTG GTTTGGAGAA GTTCAAGGAC AAAAGGGGTG GTTTCCTAAG
    TCATATGTGA AGCTTATTTC AGGCCCCATT AGGAAGTCAA CAAGCATGGA TTCTGGTTCT
    TCAGAAAGTC CTGCTAGTCT GAAAAGAGTA GCATCACCAG CAGCTAAAGC AACTATGTCA
    GGTGAAGAAT ATGTTGCTAT GTATACTTAT GAGAGTTCTG AACAAGGAGA CCTAACCTTT
    CAGCAGGGTG ATATGATTCT TGTGACAAAG AAAGATGGCG ACTGGTGGAC AGGAACACTG
    GGTGATAAAA CAGGAGTTTT CCCATCAAAT TATGTGAGAC TCAAAGATTC TGAGGCTTCT
    GGAGCAGCTG GAAAAACAGG AAGCTTAGGG AAGAAACCTG AAATTGCCCA AGTTATTGCC
    TCTTACACAG CAACGGGTCC AGAACAACTC ACCCTTGCTC CTGGTCAGTT GATTCTTATC
    AGAAAGAAGA ACCCAGGTGG CTGGTGGGAA GGAGAGCTCC AAGCACGAGG GAAAAAACGA
    CAAATAGGAT GGTTTCCTGC TAATTATGTG AAACTTTTGA GCCCTGGTAC CAGTAAAACT
    ACACCAACCG AGCTACCTAA ATCAACAGCA TTACCTTCAG TGTGCCAAGT AATTGGCATG
    TATGACTACA CTGCACAGAA TGATGATGAG CTAGCATTCA ATAAAGGCCA GATTATAAAC
    GTCCTTAACA AGGAAGACCC AGACTGGTGG AAAGGGGAGG TGAATGGACA AGTGGGTCTC
    TTCCCATCCA ACTATGTGAA GCTGACAACA GATATGGACC CAAGCCAGCA ATGGTGTGCA
    GACTTGCATC TTCTGGATAT GCTGACACCT ACTGAACGAA AAAGGCAGGG ATACATCCAT
    GAGCTCATTG TCACAGAAGA GAACTACGTA AATGATCTGC AGTTGGTCAC AGAGATCTTC
    CAGAAACCAC TAATGGAATC TGAGCTGCTA ACAGAAAAGG AAGTTGCTAT GATTTTTGTT
    AATTGGAAGG AGCTGATTAT GTGCAATATA AAACTACTGA AGGCCTTGCG TGTGCGTAAG
    AAGATGTCTG GGGAGAAGAT GCCAGTGAAA ATGATTGGTG ACATCCTGAC AGCTCAGCTG
    CCACACATGC AGCCATACAT TCGGTTCTGT AGCTGCCAGC TCAATGGGGC AGCACTCATT
    CAGCAGAAAA CAGATGAAGT CCCCGAATTC AAGGAGTTTG TAAAAAGGTT AGCAATGGAT
    CCTCGCTGTA AAGGAATGCC ACTATCAAGT TTTCTACTAA AGCCTATGCA ACGGGTAACA
    AGATACCCAC TAATAATTAA AAATATAATA GAAAACACTC CTGAAAACCA TCCTGACCAC
    AGTCACTTGA AGCATGCTCT TGAAAAAGCA GAAGAATTAT GTTCTCAAGT AAATGAAGGA
    GTGCGAGAGA AAGAAAATTC AGATAGGCTG GAGTGGATCC AGGCTCATGT TCAGTGCGAA
    GGCCTCTCTG AGCAACTCGT ATTTAATTCT GTTACAAACT GCTTGGGACC ACGCAAGTTT
    CTGCACAGTG GGAAGCTCTA TAAAGCCAAG AGTAACAAGG AACTGTATGG CTTTCTGTTC
    AACGATTTCC TCCTCTTGAC TCAGATCATA AAACCTCTTG GCTCTTCTGG CACAGACAAG
    GTCTTCAGCC CCAAGTCTAA TCTGCAGTAC AAAATGTACA AAACTCCCAT TTTTCTAAAT
    GAAGTACTAG TAAAATTACC CACAGACCCT TCTGGAGATG AGCCCATTTT CCATATCTCC
    CACATTGACA GAGTCTACAC ACTCCGGGCT GAAAGCATTA ATGAAAGGAC TGCGTGGGTT
    CAGAAAATTA AAGCTGCTTC GGAACTTTAC ATAGAGACTG AAAAGAAGAA GAGGGAAAAG
    GCCTACTTAG TTCGCTCACA AAGAGCAACA GGCATCGGGA GGTTGATGGT GAATATTGTT
    GAAGGCATTG AACTAAAACC TTGCAGATCC CATGGAAAGA GTAATCCATA CTGTGAGGTG
    ACGATGGGCT CTCAGTGCCA CATCACCAAG ACAATGCAGG ACACCCTCAA TCCAAAGTGG
    AACTCCAACT GCCAGTTCTT TATCAAAGAC CTCGAGCAGG ACGTGCTCTG CATCACGGTG
    TTTGAGAGGG ACCAGTTCTC CCCAGATGAC TTTTTGGGCA GAACAGAGAT CCGTGTGGCA
    GACATTAAGA AGGATCAGGG TTCAAAGGGA CCAGTTACTA AGTGTCTCCT TCTCCATGAA
    GTCCCAACAG GAGAGATAGT CGTCCGACTA GACCTGCAAT TGTTTGATGA GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015155482.1
    CDS213..5429
    Translation

    Target ORF information:

    RefSeq Version XM_015299996.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015299996.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGT AAGCTTTTCC AACCACATCA AGAACCAGTT
    AAGCCAGCTG TCCAGGCTCC ATGGTCAAAT GCAGGAAAAG CTCCACTAAC AATTTCTGCT
    CAGGAGGATG TAAAAATAGT GTATTATAGA GCACTGTATC CTTTTGAATC AAGAAGCCAT
    GACGAAATCA CTATTCAACC TGGCGACATA GTCATGGTGG ATGAAAGTCA GACTGGAGAA
    CCAGGGTGGC TTGGTGGAGA ATTGAAGGGA AAAACTGGAT GGTTCCCAGC AAACTACGCA
    GAAAAAATAC CTGAAAGTGA AGTTCCAGCA TCTATAAAAC CAGTTGAGGC AGCTCCTGCA
    CCTAAAGTTT CTGTGCATGA AACAACCACA TCACTAGGAA CTGCTGCATC TACAGAGTGC
    ACGACAACTG CTAACAACTG GGCAGACTTC AGTTCAACAT GGCCAGCAAA CACTAATGAG
    AAACCAGAGA CAGACAACTG GGATGCCTGG GCAGCTCAAC CATCTTTGAC TGTTCCCAGT
    GCAGGGCAGC TGCGGCAGCG ATCAGCCTTC ACCCCCGCCA CTGTTACAGG ATCGTCTCCC
    TCTCCTGTAT TGGGTCAGGG TGAAAAAGTG GAGGGACTTC AAGCACAGGC TCTGTATCCT
    TGGAGAGCAA AAAAAGACAA CCACCTAAAT TTCAACAAAA ATGATGTCAT CACAGTTTTA
    GAGCAGCAAG ATATGTGGTG GTTTGGAGAA GTTCAAGGAC AAAAGGGGTG GTTTCCTAAG
    TCATATGTGA AGCTTATTTC AGGCCCCATT AGGAAGTCAA CAAGCATGGA TTCTGGTTCT
    TCAGAAAGTC CTGCTAGTCT GAAAAGAGTA GCATCACCAG CAGCTAAAGC AACTATGTCA
    GGTGAAGAAT ATGTTGCTAT GTATACTTAT GAGAGTTCTG AACAAGGAGA CCTAACCTTT
    CAGCAGGGTG ATATGATTCT TGTGACAAAG AAAGATGGCG ACTGGTGGAC AGGAACACTG
    GGTGATAAAA CAGGAGTTTT CCCATCAAAT TATGTGAGAC TCAAAGATTC TGAGGCTTCT
    GGAGCAGCTG GAAAAACAGG AAGCTTAGGG AAGAAACCTG AAATTGCCCA AGTTATTGCC
    TCTTACACAG CAACGGGTCC AGAACAACTC ACCCTTGCTC CTGGTCAGTT GATTCTTATC
    AGAAAGAAGA ACCCAGGTGG CTGGTGGGAA GGAGAGCTCC AAGCACGAGG GAAAAAACGA
    CAAATAGGAT GGTTTCCTGC TAATTATGTG AAACTTTTGA GCCCTGGTAC CAGTAAAACT
    ACACCAACCG AGCTACCTAA ATCAACAGCA TTACCTTCAG TGTGCCAAGT AATTGGCATG
    TATGACTACA CTGCACAGAA TGATGATGAG CTAGCATTCA ATAAAGGCCA GATTATAAAC
    GTCCTTAACA AGGAAGACCC AGACTGGTGG AAAGGGGAGG TGAATGGACA AGTGGGTCTC
    TTCCCATCCA ACTATGTGAA GCTGACAACA GATATGGACC CAAGCCAGCA ATGGTGTGCA
    GACTTGCATC TTCTGGATAT GCTGACACCT ACTGAACGAA AAAGGCAGGG ATACATCCAT
    GAGCTCATTG TCACAGAAGA GAACTACGTA AATGATCTGC AGTTGGTCAC AGAGATCTTC
    CAGAAACCAC TAATGGAATC TGAGCTGCTA ACAGAAAAGG AAGTTGCTAT GATTTTTGTT
    AATTGGAAGG AGCTGATTAT GTGCAATATA AAACTACTGA AGGCCTTGCG TGTGCGTAAG
    AAGATGTCTG GGGAGAAGAT GCCAGTGAAA ATGATTGGTG ACATCCTGAC AGCTCAGCTG
    CCACACATGC AGCCATACAT TCGGTTCTGT AGCTGCCAGC TCAATGGGGC AGCACTCATT
    CAGCAGAAAA CAGATGAAGT CCCCGAATTC AAGGAGTTTG TAAAAAGGTT AGCAATGGAT
    CCTCGCTGTA AAGGAATGCC ACTATCAAGT TTTCTACTAA AGCCTATGCA ACGGGTAACA
    AGATACCCAC TAATAATTAA AAATATAATA GAAAACACTC CTGAAAACCA TCCTGACCAC
    AGTCACTTGA AGCATGCTCT TGAAAAAGCA GAAGAATTAT GTTCTCAAGT AAATGAAGGA
    GTGCGAGAGA AAGAAAATTC AGATAGGCTG GAGTGGATCC AGGCTCATGT TCAGTGCGAA
    GGCCTCTCTG AGCAACTCGT ATTTAATTCT GTTACAAACT GCTTGGGACC ACGCAAGTTT
    CTGCACAGTG GGAAGCTCTA TAAAGCCAAG AGTAACAAGG AACTGTATGG CTTTCTGTTC
    AACGATTTCC TCCTCTTGAC TCAGATCATA AAACCTCTTG GCTCTTCTGG CACAGACAAG
    GTCTTCAGCC CCAAGTCTAA TCTGCAGTAC AAAATGTACA AAACTCCCAT TTTTCTAAAT
    GAAGTACTAG TAAAATTACC CACAGACCCT TCTGGAGATG AGCCCATTTT CCATATCTCC
    CACATTGACA GAGTCTACAC ACTCCGGGCT GAAAGCATTA ATGAAAGGAC TGCGTGGGTT
    CAGAAAATTA AAGCTGCTTC GGAACTTTAC ATAGAGACTG AAAAGAAGAA GAGGGAAAAG
    GCCTACTTAG TTCGCTCACA AAGAGCAACA GGCATCGGGA GGTTGATGGT GAATATTGTT
    GAAGGCATTG AACTAAAACC TTGCAGATCC CATGGAAAGA GTAATCCATA CTGTGAGGTG
    ACGATGGGCT CTCAGTGCCA CATCACCAAG ACAATGCAGG ACACCCTCAA TCCAAAGTGG
    AACTCCAACT GCCAGTTCTT TATCAAAGAC CTCGAGCAGG ACGTGCTCTG CATCACGGTG
    TTTGAGAGGG ACCAGTTCTC CCCAGATGAC TTTTTGGGCA GAACAGAGAT CCGTGTGGCA
    GACATTAAGA AGGATCAGGG TTCAAAGGGA CCAGTTACTA AGTGTCTCCT TCTCCATGAA
    GTCCCAACAG GAGAGATAGT CGTCCGACTA GACCTGCAAT TGTTTGATGA GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30285
    Clone ID Related Accession (Same CDS sequence) XM_015299996.1 , XM_015299996.2
    Accession Version XM_015299996.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product intersectin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015299996.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGT AAGCTTTTCC AACCACATCA AGAACCAGTT
    AAGCCAGCTG TCCAGGCTCC ATGGTCAAAT GCAGGAAAAG CTCCACTAAC AATTTCTGCT
    CAGGAGGATG TAAAAATAGT GTATTATAGA GCACTGTATC CTTTTGAATC AAGAAGCCAT
    GACGAAATCA CTATTCAACC TGGCGACATA GTCATGGTGG ATGAAAGTCA GACTGGAGAA
    CCAGGGTGGC TTGGTGGAGA ATTGAAGGGA AAAACTGGAT GGTTCCCAGC AAACTACGCA
    GAAAAAATAC CTGAAAGTGA AGTTCCAGCA TCTATAAAAC CAGTTGAGGC AGCTCCTGCA
    CCTAAAGTTT CTGTGCATGA AACAACCACA TCACTAGGAA CTGCTGCATC TACAGAGTGC
    ACGACAACTG CTAACAACTG GGCAGACTTC AGTTCAACAT GGCCAGCAAA CACTAATGAG
    AAACCAGAGA CAGACAACTG GGATGCCTGG GCAGCTCAAC CATCTTTGAC TGTTCCCAGT
    GCAGGGCAGC TGCGGCAGCG ATCAGCCTTC ACCCCCGCCA CTGTTACAGG ATCGTCTCCC
    TCTCCTGTAT TGGGTCAGGG TGAAAAAGTG GAGGGACTTC AAGCACAGGC TCTGTATCCT
    TGGAGAGCAA AAAAAGACAA CCACCTAAAT TTCAACAAAA ATGATGTCAT CACAGTTTTA
    GAGCAGCAAG ATATGTGGTG GTTTGGAGAA GTTCAAGGAC AAAAGGGGTG GTTTCCTAAG
    TCATATGTGA AGCTTATTTC AGGCCCCATT AGGAAGTCAA CAAGCATGGA TTCTGGTTCT
    TCAGAAAGTC CTGCTAGTCT GAAAAGAGTA GCATCACCAG CAGCTAAAGC AACTATGTCA
    GGTGAAGAAT ATGTTGCTAT GTATACTTAT GAGAGTTCTG AACAAGGAGA CCTAACCTTT
    CAGCAGGGTG ATATGATTCT TGTGACAAAG AAAGATGGCG ACTGGTGGAC AGGAACACTG
    GGTGATAAAA CAGGAGTTTT CCCATCAAAT TATGTGAGAC TCAAAGATTC TGAGGCTTCT
    GGAGCAGCTG GAAAAACAGG AAGCTTAGGG AAGAAACCTG AAATTGCCCA AGTTATTGCC
    TCTTACACAG CAACGGGTCC AGAACAACTC ACCCTTGCTC CTGGTCAGTT GATTCTTATC
    AGAAAGAAGA ACCCAGGTGG CTGGTGGGAA GGAGAGCTCC AAGCACGAGG GAAAAAACGA
    CAAATAGGAT GGTTTCCTGC TAATTATGTG AAACTTTTGA GCCCTGGTAC CAGTAAAACT
    ACACCAACCG AGCTACCTAA ATCAACAGCA TTACCTTCAG TGTGCCAAGT AATTGGCATG
    TATGACTACA CTGCACAGAA TGATGATGAG CTAGCATTCA ATAAAGGCCA GATTATAAAC
    GTCCTTAACA AGGAAGACCC AGACTGGTGG AAAGGGGAGG TGAATGGACA AGTGGGTCTC
    TTCCCATCCA ACTATGTGAA GCTGACAACA GATATGGACC CAAGCCAGCA ATGGTGTGCA
    GACTTGCATC TTCTGGATAT GCTGACACCT ACTGAACGAA AAAGGCAGGG ATACATCCAT
    GAGCTCATTG TCACAGAAGA GAACTACGTA AATGATCTGC AGTTGGTCAC AGAGATCTTC
    CAGAAACCAC TAATGGAATC TGAGCTGCTA ACAGAAAAGG AAGTTGCTAT GATTTTTGTT
    AATTGGAAGG AGCTGATTAT GTGCAATATA AAACTACTGA AGGCCTTGCG TGTGCGTAAG
    AAGATGTCTG GGGAGAAGAT GCCAGTGAAA ATGATTGGTG ACATCCTGAC AGCTCAGCTG
    CCACACATGC AGCCATACAT TCGGTTCTGT AGCTGCCAGC TCAATGGGGC AGCACTCATT
    CAGCAGAAAA CAGATGAAGT CCCCGAATTC AAGGAGTTTG TAAAAAGGTT AGCAATGGAT
    CCTCGCTGTA AAGGAATGCC ACTATCAAGT TTTCTACTAA AGCCTATGCA ACGGGTAACA
    AGATACCCAC TAATAATTAA AAATATAATA GAAAACACTC CTGAAAACCA TCCTGACCAC
    AGTCACTTGA AGCATGCTCT TGAAAAAGCA GAAGAATTAT GTTCTCAAGT AAATGAAGGA
    GTGCGAGAGA AAGAAAATTC AGATAGGCTG GAGTGGATCC AGGCTCATGT TCAGTGCGAA
    GGCCTCTCTG AGCAACTCGT ATTTAATTCT GTTACAAACT GCTTGGGACC ACGCAAGTTT
    CTGCACAGTG GGAAGCTCTA TAAAGCCAAG AGTAACAAGG AACTGTATGG CTTTCTGTTC
    AACGATTTCC TCCTCTTGAC TCAGATCATA AAACCTCTTG GCTCTTCTGG CACAGACAAG
    GTCTTCAGCC CCAAGTCTAA TCTGCAGTAC AAAATGTACA AAACTCCCAT TTTTCTAAAT
    GAAGTACTAG TAAAATTACC CACAGACCCT TCTGGAGATG AGCCCATTTT CCATATCTCC
    CACATTGACA GAGTCTACAC ACTCCGGGCT GAAAGCATTA ATGAAAGGAC TGCGTGGGTT
    CAGAAAATTA AAGCTGCTTC GGAACTTTAC ATAGAGACTG AAAAGAAGAA GAGGGAAAAG
    GCCTACTTAG TTCGCTCACA AAGAGCAACA GGCATCGGGA GGTTGATGGT GAATATTGTT
    GAAGGCATTG AACTAAAACC TTGCAGATCC CATGGAAAGA GTAATCCATA CTGTGAGGTG
    ACGATGGGCT CTCAGTGCCA CATCACCAAG ACAATGCAGG ACACCCTCAA TCCAAAGTGG
    AACTCCAACT GCCAGTTCTT TATCAAAGAC CTCGAGCAGG ACGTGCTCTG CATCACGGTG
    TTTGAGAGGG ACCAGTTCTC CCCAGATGAC TTTTTGGGCA GAACAGAGAT CCGTGTGGCA
    GACATTAAGA AGGATCAGGG TTCAAAGGGA CCAGTTACTA AGTGTCTCCT TCTCCATGAA
    GTCCCAACAG GAGAGATAGT CGTCCGACTA GACCTGCAAT TGTTTGATGA GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015155482.1
    CDS231..5447
    Translation

    Target ORF information:

    RefSeq Version XM_015299996.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus intersectin 1 (ITSN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015299996.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGT AAGCTTTTCC AACCACATCA AGAACCAGTT
    AAGCCAGCTG TCCAGGCTCC ATGGTCAAAT GCAGGAAAAG CTCCACTAAC AATTTCTGCT
    CAGGAGGATG TAAAAATAGT GTATTATAGA GCACTGTATC CTTTTGAATC AAGAAGCCAT
    GACGAAATCA CTATTCAACC TGGCGACATA GTCATGGTGG ATGAAAGTCA GACTGGAGAA
    CCAGGGTGGC TTGGTGGAGA ATTGAAGGGA AAAACTGGAT GGTTCCCAGC AAACTACGCA
    GAAAAAATAC CTGAAAGTGA AGTTCCAGCA TCTATAAAAC CAGTTGAGGC AGCTCCTGCA
    CCTAAAGTTT CTGTGCATGA AACAACCACA TCACTAGGAA CTGCTGCATC TACAGAGTGC
    ACGACAACTG CTAACAACTG GGCAGACTTC AGTTCAACAT GGCCAGCAAA CACTAATGAG
    AAACCAGAGA CAGACAACTG GGATGCCTGG GCAGCTCAAC CATCTTTGAC TGTTCCCAGT
    GCAGGGCAGC TGCGGCAGCG ATCAGCCTTC ACCCCCGCCA CTGTTACAGG ATCGTCTCCC
    TCTCCTGTAT TGGGTCAGGG TGAAAAAGTG GAGGGACTTC AAGCACAGGC TCTGTATCCT
    TGGAGAGCAA AAAAAGACAA CCACCTAAAT TTCAACAAAA ATGATGTCAT CACAGTTTTA
    GAGCAGCAAG ATATGTGGTG GTTTGGAGAA GTTCAAGGAC AAAAGGGGTG GTTTCCTAAG
    TCATATGTGA AGCTTATTTC AGGCCCCATT AGGAAGTCAA CAAGCATGGA TTCTGGTTCT
    TCAGAAAGTC CTGCTAGTCT GAAAAGAGTA GCATCACCAG CAGCTAAAGC AACTATGTCA
    GGTGAAGAAT ATGTTGCTAT GTATACTTAT GAGAGTTCTG AACAAGGAGA CCTAACCTTT
    CAGCAGGGTG ATATGATTCT TGTGACAAAG AAAGATGGCG ACTGGTGGAC AGGAACACTG
    GGTGATAAAA CAGGAGTTTT CCCATCAAAT TATGTGAGAC TCAAAGATTC TGAGGCTTCT
    GGAGCAGCTG GAAAAACAGG AAGCTTAGGG AAGAAACCTG AAATTGCCCA AGTTATTGCC
    TCTTACACAG CAACGGGTCC AGAACAACTC ACCCTTGCTC CTGGTCAGTT GATTCTTATC
    AGAAAGAAGA ACCCAGGTGG CTGGTGGGAA GGAGAGCTCC AAGCACGAGG GAAAAAACGA
    CAAATAGGAT GGTTTCCTGC TAATTATGTG AAACTTTTGA GCCCTGGTAC CAGTAAAACT
    ACACCAACCG AGCTACCTAA ATCAACAGCA TTACCTTCAG TGTGCCAAGT AATTGGCATG
    TATGACTACA CTGCACAGAA TGATGATGAG CTAGCATTCA ATAAAGGCCA GATTATAAAC
    GTCCTTAACA AGGAAGACCC AGACTGGTGG AAAGGGGAGG TGAATGGACA AGTGGGTCTC
    TTCCCATCCA ACTATGTGAA GCTGACAACA GATATGGACC CAAGCCAGCA ATGGTGTGCA
    GACTTGCATC TTCTGGATAT GCTGACACCT ACTGAACGAA AAAGGCAGGG ATACATCCAT
    GAGCTCATTG TCACAGAAGA GAACTACGTA AATGATCTGC AGTTGGTCAC AGAGATCTTC
    CAGAAACCAC TAATGGAATC TGAGCTGCTA ACAGAAAAGG AAGTTGCTAT GATTTTTGTT
    AATTGGAAGG AGCTGATTAT GTGCAATATA AAACTACTGA AGGCCTTGCG TGTGCGTAAG
    AAGATGTCTG GGGAGAAGAT GCCAGTGAAA ATGATTGGTG ACATCCTGAC AGCTCAGCTG
    CCACACATGC AGCCATACAT TCGGTTCTGT AGCTGCCAGC TCAATGGGGC AGCACTCATT
    CAGCAGAAAA CAGATGAAGT CCCCGAATTC AAGGAGTTTG TAAAAAGGTT AGCAATGGAT
    CCTCGCTGTA AAGGAATGCC ACTATCAAGT TTTCTACTAA AGCCTATGCA ACGGGTAACA
    AGATACCCAC TAATAATTAA AAATATAATA GAAAACACTC CTGAAAACCA TCCTGACCAC
    AGTCACTTGA AGCATGCTCT TGAAAAAGCA GAAGAATTAT GTTCTCAAGT AAATGAAGGA
    GTGCGAGAGA AAGAAAATTC AGATAGGCTG GAGTGGATCC AGGCTCATGT TCAGTGCGAA
    GGCCTCTCTG AGCAACTCGT ATTTAATTCT GTTACAAACT GCTTGGGACC ACGCAAGTTT
    CTGCACAGTG GGAAGCTCTA TAAAGCCAAG AGTAACAAGG AACTGTATGG CTTTCTGTTC
    AACGATTTCC TCCTCTTGAC TCAGATCATA AAACCTCTTG GCTCTTCTGG CACAGACAAG
    GTCTTCAGCC CCAAGTCTAA TCTGCAGTAC AAAATGTACA AAACTCCCAT TTTTCTAAAT
    GAAGTACTAG TAAAATTACC CACAGACCCT TCTGGAGATG AGCCCATTTT CCATATCTCC
    CACATTGACA GAGTCTACAC ACTCCGGGCT GAAAGCATTA ATGAAAGGAC TGCGTGGGTT
    CAGAAAATTA AAGCTGCTTC GGAACTTTAC ATAGAGACTG AAAAGAAGAA GAGGGAAAAG
    GCCTACTTAG TTCGCTCACA AAGAGCAACA GGCATCGGGA GGTTGATGGT GAATATTGTT
    GAAGGCATTG AACTAAAACC TTGCAGATCC CATGGAAAGA GTAATCCATA CTGTGAGGTG
    ACGATGGGCT CTCAGTGCCA CATCACCAAG ACAATGCAGG ACACCCTCAA TCCAAAGTGG
    AACTCCAACT GCCAGTTCTT TATCAAAGAC CTCGAGCAGG ACGTGCTCTG CATCACGGTG
    TTTGAGAGGG ACCAGTTCTC CCCAGATGAC TTTTTGGGCA GAACAGAGAT CCGTGTGGCA
    GACATTAAGA AGGATCAGGG TTCAAAGGGA CCAGTTACTA AGTGTCTCCT TCTCCATGAA
    GTCCCAACAG GAGAGATAGT CGTCCGACTA GACCTGCAAT TGTTTGATGA GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30286
    Clone ID Related Accession (Same CDS sequence) XM_015300003.2 , XM_015300003.1
    Accession Version XM_015300003.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5166bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product intersectin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015300003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGA AAAGCTCCAC TAACAATTTC TGCTCAGGAG
    GATGTAAAAA TAGTGTATTA TAGAGCACTG TATCCTTTTG AATCAAGAAG CCATGACGAA
    ATCACTATTC AACCTGGCGA CATAGTCATG GTTAAAAGGG AATGGGTGGA TGAAAGTCAG
    ACTGGAGAAC CAGGGTGGCT TGGTGGAGAA TTGAAGGGAA AAACTGGATG GTTCCCAGCA
    AACTACGCAG AAAAAATACC TGAAAGTGAA GTTCCAGCAT CTATAAAACC AGTTGAGGCA
    GCTCCTGCAC CTAAAGTTTC TGTGCATGAA ACAACCACAT CACTAGGAAC TGCTGCATCT
    ACAGAGTGCA CGACAACTGC TAACAACTGG GCAGACTTCA GTTCAACATG GCCAGCAAAC
    ACTAATGAGA AACCAGAGAC AGACAACTGG GATGCCTGGG CAGCTCAACC ATCTTTGACT
    GTTCCCAGTG CAGGGCAGCT GCGGCAGCGA TCAGCCTTCA CCCCCGCCAC TGTTACAGGA
    TCGTCTCCCT CTCCTGTATT GGGTCAGGGT GAAAAAGTGG AGGGACTTCA AGCACAGGCT
    CTGTATCCTT GGAGAGCAAA AAAAGACAAC CACCTAAATT TCAACAAAAA TGATGTCATC
    ACAGTTTTAG AGCAGCAAGA TATGTGGTGG TTTGGAGAAG TTCAAGGACA AAAGGGGTGG
    TTTCCTAAGT CATATGTGAA GCTTATTTCA GGCCCCATTA GGAAGTCAAC AAGCATGGAT
    TCTGGTTCTT CAGAAAGTCC TGCTAGTCTG AAAAGAGTAG CATCACCAGC AGCTAAAGCA
    ACTATGTCAG GTGAAGAATA TGTTGCTATG TATACTTATG AGAGTTCTGA ACAAGGAGAC
    CTAACCTTTC AGCAGGGTGA TATGATTCTT GTGACAAAGA AAGATGGCGA CTGGTGGACA
    GGAACACTGG GTGATAAAAC AGGAGTTTTC CCATCAAATT ATGTGAGACT CAAAGATTCT
    GAGGCTTCTG GAGCAGCTGG AAAAACAGGA AGCTTAGGGA AGAAACCTGA AATTGCCCAA
    GTTATTGCCT CTTACACAGC AACGGGTCCA GAACAACTCA CCCTTGCTCC TGGTCAGTTG
    ATTCTTATCA GAAAGAAGAA CCCAGGTGGC TGGTGGGAAG GAGAGCTCCA AGCACGAGGG
    AAAAAACGAC AAATAGGATG GTTTCCTGCT AATTATGTGA AACTTTTGAG CCCTGGTACC
    AGTAAAACTA CACCAACCGA GCTACCTAAA TCAACAGCAT TACCTTCAGT GTGCCAAGTA
    ATTGGCATGT ATGACTACAC TGCACAGAAT GATGATGAGC TAGCATTCAA TAAAGGCCAG
    ATTATAAACG TCCTTAACAA GGAAGACCCA GACTGGTGGA AAGGGGAGGT GAATGGACAA
    GTGGGTCTCT TCCCATCCAA CTATGTGAAG CTGACAACAG ATATGGACCC AAGCCAGCAA
    TGGTGTGCAG ACTTGCATCT TCTGGATATG CTGACACCTA CTGAACGAAA AAGGCAGGGA
    TACATCCATG AGCTCATTGT CACAGAAGAG AACTACGTAA ATGATCTGCA GTTGGTCACA
    GAGATCTTCC AGAAACCACT AATGGAATCT GAGCTGCTAA CAGAAAAGGA AGTTGCTATG
    ATTTTTGTTA ATTGGAAGGA GCTGATTATG TGCAATATAA AACTACTGAA GGCCTTGCGT
    GTGCGTAAGA AGATGTCTGG GGAGAAGATG CCAGTGAAAA TGATTGGTGA CATCCTGACA
    GCTCAGCTGC CACACATGCA GCCATACATT CGGTTCTGTA GCTGCCAGCT CAATGGGGCA
    GCACTCATTC AGCAGAAAAC AGATGAAGTC CCCGAATTCA AGGAGTTTGT AAAAAGGTTA
    GCAATGGATC CTCGCTGTAA AGGAATGCCA CTATCAAGTT TTCTACTAAA GCCTATGCAA
    CGGGTAACAA GATACCCACT AATAATTAAA AATATAATAG AAAACACTCC TGAAAACCAT
    CCTGACCACA GTCACTTGAA GCATGCTCTT GAAAAAGCAG AAGAATTATG TTCTCAAGTA
    AATGAAGGAG TGCGAGAGAA AGAAAATTCA GATAGGCTGG AGTGGATCCA GGCTCATGTT
    CAGTGCGAAG GCCTCTCTGA GCAACTCGTA TTTAATTCTG TTACAAACTG CTTGGGACCA
    CGCAAGTTTC TGCACAGTGG GAAGCTCTAT AAAGCCAAGA GTAACAAGGA ACTGTATGGC
    TTTCTGTTCA ACGATTTCCT CCTCTTGACT CAGATCATAA AACCTCTTGG CTCTTCTGGC
    ACAGACAAGG TCTTCAGCCC CAAGTCTAAT CTGCAGTACA AAATGTACAA AACTCCCATT
    TTTCTAAATG AAGTACTAGT AAAATTACCC ACAGACCCTT CTGGAGATGA GCCCATTTTC
    CATATCTCCC ACATTGACAG AGTCTACACA CTCCGGGCTG AAAGCATTAA TGAAAGGACT
    GCGTGGGTTC AGAAAATTAA AGCTGCTTCG GAACTTTACA TAGAGACTGA AAAGAAGAAG
    AGGGAAAAGG CCTACTTAGT TCGCTCACAA AGAGCAACAG GCATCGGGAG GTTGATGGTG
    AATATTGTTG AAGGCATTGA ACTAAAACCT TGCAGATCCC ATGGAAAGAG TAATCCATAC
    TGTGAGGTGA CGATGGGCTC TCAGTGCCAC ATCACCAAGA CAATGCAGGA CACCCTCAAT
    CCAAAGTGGA ACTCCAACTG CCAGTTCTTT ATCAAAGACC TCGAGCAGGA CGTGCTCTGC
    ATCACGGTGT TTGAGAGGGA CCAGTTCTCC CCAGATGACT TTTTGGGCAG AACAGAGATC
    CGTGTGGCAG ACATTAAGAA GGATCAGGGT TCAAAGGGAC CAGTTACTAA GTGTCTCCTT
    CTCCATGAAG TCCCAACAGG AGAGATAGTC GTCCGACTAG ACCTGCAATT GTTTGATGAG
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015155489.1
    CDS232..5397
    Translation

    Target ORF information:

    RefSeq Version XM_015300003.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus intersectin 1 (ITSN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015300003.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGA AAAGCTCCAC TAACAATTTC TGCTCAGGAG
    GATGTAAAAA TAGTGTATTA TAGAGCACTG TATCCTTTTG AATCAAGAAG CCATGACGAA
    ATCACTATTC AACCTGGCGA CATAGTCATG GTTAAAAGGG AATGGGTGGA TGAAAGTCAG
    ACTGGAGAAC CAGGGTGGCT TGGTGGAGAA TTGAAGGGAA AAACTGGATG GTTCCCAGCA
    AACTACGCAG AAAAAATACC TGAAAGTGAA GTTCCAGCAT CTATAAAACC AGTTGAGGCA
    GCTCCTGCAC CTAAAGTTTC TGTGCATGAA ACAACCACAT CACTAGGAAC TGCTGCATCT
    ACAGAGTGCA CGACAACTGC TAACAACTGG GCAGACTTCA GTTCAACATG GCCAGCAAAC
    ACTAATGAGA AACCAGAGAC AGACAACTGG GATGCCTGGG CAGCTCAACC ATCTTTGACT
    GTTCCCAGTG CAGGGCAGCT GCGGCAGCGA TCAGCCTTCA CCCCCGCCAC TGTTACAGGA
    TCGTCTCCCT CTCCTGTATT GGGTCAGGGT GAAAAAGTGG AGGGACTTCA AGCACAGGCT
    CTGTATCCTT GGAGAGCAAA AAAAGACAAC CACCTAAATT TCAACAAAAA TGATGTCATC
    ACAGTTTTAG AGCAGCAAGA TATGTGGTGG TTTGGAGAAG TTCAAGGACA AAAGGGGTGG
    TTTCCTAAGT CATATGTGAA GCTTATTTCA GGCCCCATTA GGAAGTCAAC AAGCATGGAT
    TCTGGTTCTT CAGAAAGTCC TGCTAGTCTG AAAAGAGTAG CATCACCAGC AGCTAAAGCA
    ACTATGTCAG GTGAAGAATA TGTTGCTATG TATACTTATG AGAGTTCTGA ACAAGGAGAC
    CTAACCTTTC AGCAGGGTGA TATGATTCTT GTGACAAAGA AAGATGGCGA CTGGTGGACA
    GGAACACTGG GTGATAAAAC AGGAGTTTTC CCATCAAATT ATGTGAGACT CAAAGATTCT
    GAGGCTTCTG GAGCAGCTGG AAAAACAGGA AGCTTAGGGA AGAAACCTGA AATTGCCCAA
    GTTATTGCCT CTTACACAGC AACGGGTCCA GAACAACTCA CCCTTGCTCC TGGTCAGTTG
    ATTCTTATCA GAAAGAAGAA CCCAGGTGGC TGGTGGGAAG GAGAGCTCCA AGCACGAGGG
    AAAAAACGAC AAATAGGATG GTTTCCTGCT AATTATGTGA AACTTTTGAG CCCTGGTACC
    AGTAAAACTA CACCAACCGA GCTACCTAAA TCAACAGCAT TACCTTCAGT GTGCCAAGTA
    ATTGGCATGT ATGACTACAC TGCACAGAAT GATGATGAGC TAGCATTCAA TAAAGGCCAG
    ATTATAAACG TCCTTAACAA GGAAGACCCA GACTGGTGGA AAGGGGAGGT GAATGGACAA
    GTGGGTCTCT TCCCATCCAA CTATGTGAAG CTGACAACAG ATATGGACCC AAGCCAGCAA
    TGGTGTGCAG ACTTGCATCT TCTGGATATG CTGACACCTA CTGAACGAAA AAGGCAGGGA
    TACATCCATG AGCTCATTGT CACAGAAGAG AACTACGTAA ATGATCTGCA GTTGGTCACA
    GAGATCTTCC AGAAACCACT AATGGAATCT GAGCTGCTAA CAGAAAAGGA AGTTGCTATG
    ATTTTTGTTA ATTGGAAGGA GCTGATTATG TGCAATATAA AACTACTGAA GGCCTTGCGT
    GTGCGTAAGA AGATGTCTGG GGAGAAGATG CCAGTGAAAA TGATTGGTGA CATCCTGACA
    GCTCAGCTGC CACACATGCA GCCATACATT CGGTTCTGTA GCTGCCAGCT CAATGGGGCA
    GCACTCATTC AGCAGAAAAC AGATGAAGTC CCCGAATTCA AGGAGTTTGT AAAAAGGTTA
    GCAATGGATC CTCGCTGTAA AGGAATGCCA CTATCAAGTT TTCTACTAAA GCCTATGCAA
    CGGGTAACAA GATACCCACT AATAATTAAA AATATAATAG AAAACACTCC TGAAAACCAT
    CCTGACCACA GTCACTTGAA GCATGCTCTT GAAAAAGCAG AAGAATTATG TTCTCAAGTA
    AATGAAGGAG TGCGAGAGAA AGAAAATTCA GATAGGCTGG AGTGGATCCA GGCTCATGTT
    CAGTGCGAAG GCCTCTCTGA GCAACTCGTA TTTAATTCTG TTACAAACTG CTTGGGACCA
    CGCAAGTTTC TGCACAGTGG GAAGCTCTAT AAAGCCAAGA GTAACAAGGA ACTGTATGGC
    TTTCTGTTCA ACGATTTCCT CCTCTTGACT CAGATCATAA AACCTCTTGG CTCTTCTGGC
    ACAGACAAGG TCTTCAGCCC CAAGTCTAAT CTGCAGTACA AAATGTACAA AACTCCCATT
    TTTCTAAATG AAGTACTAGT AAAATTACCC ACAGACCCTT CTGGAGATGA GCCCATTTTC
    CATATCTCCC ACATTGACAG AGTCTACACA CTCCGGGCTG AAAGCATTAA TGAAAGGACT
    GCGTGGGTTC AGAAAATTAA AGCTGCTTCG GAACTTTACA TAGAGACTGA AAAGAAGAAG
    AGGGAAAAGG CCTACTTAGT TCGCTCACAA AGAGCAACAG GCATCGGGAG GTTGATGGTG
    AATATTGTTG AAGGCATTGA ACTAAAACCT TGCAGATCCC ATGGAAAGAG TAATCCATAC
    TGTGAGGTGA CGATGGGCTC TCAGTGCCAC ATCACCAAGA CAATGCAGGA CACCCTCAAT
    CCAAAGTGGA ACTCCAACTG CCAGTTCTTT ATCAAAGACC TCGAGCAGGA CGTGCTCTGC
    ATCACGGTGT TTGAGAGGGA CCAGTTCTCC CCAGATGACT TTTTGGGCAG AACAGAGATC
    CGTGTGGCAG ACATTAAGAA GGATCAGGGT TCAAAGGGAC CAGTTACTAA GTGTCTCCTT
    CTCCATGAAG TCCCAACAGG AGAGATAGTC GTCCGACTAG ACCTGCAATT GTTTGATGAG
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30286
    Clone ID Related Accession (Same CDS sequence) XM_015300003.2 , XM_015300003.1
    Accession Version XM_015300003.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5166bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product intersectin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455737.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGA AAAGCTCCAC TAACAATTTC TGCTCAGGAG
    GATGTAAAAA TAGTGTATTA TAGAGCACTG TATCCTTTTG AATCAAGAAG CCATGACGAA
    ATCACTATTC AACCTGGCGA CATAGTCATG GTTAAAAGGG AATGGGTGGA TGAAAGTCAG
    ACTGGAGAAC CAGGGTGGCT TGGTGGAGAA TTGAAGGGAA AAACTGGATG GTTCCCAGCA
    AACTACGCAG AAAAAATACC TGAAAGTGAA GTTCCAGCAT CTATAAAACC AGTTGAGGCA
    GCTCCTGCAC CTAAAGTTTC TGTGCATGAA ACAACCACAT CACTAGGAAC TGCTGCATCT
    ACAGAGTGCA CGACAACTGC TAACAACTGG GCAGACTTCA GTTCAACATG GCCAGCAAAC
    ACTAATGAGA AACCAGAGAC AGACAACTGG GATGCCTGGG CAGCTCAACC ATCTTTGACT
    GTTCCCAGTG CAGGGCAGCT GCGGCAGCGA TCAGCCTTCA CCCCCGCCAC TGTTACAGGA
    TCGTCTCCCT CTCCTGTATT GGGTCAGGGT GAAAAAGTGG AGGGACTTCA AGCACAGGCT
    CTGTATCCTT GGAGAGCAAA AAAAGACAAC CACCTAAATT TCAACAAAAA TGATGTCATC
    ACAGTTTTAG AGCAGCAAGA TATGTGGTGG TTTGGAGAAG TTCAAGGACA AAAGGGGTGG
    TTTCCTAAGT CATATGTGAA GCTTATTTCA GGCCCCATTA GGAAGTCAAC AAGCATGGAT
    TCTGGTTCTT CAGAAAGTCC TGCTAGTCTG AAAAGAGTAG CATCACCAGC AGCTAAAGCA
    ACTATGTCAG GTGAAGAATA TGTTGCTATG TATACTTATG AGAGTTCTGA ACAAGGAGAC
    CTAACCTTTC AGCAGGGTGA TATGATTCTT GTGACAAAGA AAGATGGCGA CTGGTGGACA
    GGAACACTGG GTGATAAAAC AGGAGTTTTC CCATCAAATT ATGTGAGACT CAAAGATTCT
    GAGGCTTCTG GAGCAGCTGG AAAAACAGGA AGCTTAGGGA AGAAACCTGA AATTGCCCAA
    GTTATTGCCT CTTACACAGC AACGGGTCCA GAACAACTCA CCCTTGCTCC TGGTCAGTTG
    ATTCTTATCA GAAAGAAGAA CCCAGGTGGC TGGTGGGAAG GAGAGCTCCA AGCACGAGGG
    AAAAAACGAC AAATAGGATG GTTTCCTGCT AATTATGTGA AACTTTTGAG CCCTGGTACC
    AGTAAAACTA CACCAACCGA GCTACCTAAA TCAACAGCAT TACCTTCAGT GTGCCAAGTA
    ATTGGCATGT ATGACTACAC TGCACAGAAT GATGATGAGC TAGCATTCAA TAAAGGCCAG
    ATTATAAACG TCCTTAACAA GGAAGACCCA GACTGGTGGA AAGGGGAGGT GAATGGACAA
    GTGGGTCTCT TCCCATCCAA CTATGTGAAG CTGACAACAG ATATGGACCC AAGCCAGCAA
    TGGTGTGCAG ACTTGCATCT TCTGGATATG CTGACACCTA CTGAACGAAA AAGGCAGGGA
    TACATCCATG AGCTCATTGT CACAGAAGAG AACTACGTAA ATGATCTGCA GTTGGTCACA
    GAGATCTTCC AGAAACCACT AATGGAATCT GAGCTGCTAA CAGAAAAGGA AGTTGCTATG
    ATTTTTGTTA ATTGGAAGGA GCTGATTATG TGCAATATAA AACTACTGAA GGCCTTGCGT
    GTGCGTAAGA AGATGTCTGG GGAGAAGATG CCAGTGAAAA TGATTGGTGA CATCCTGACA
    GCTCAGCTGC CACACATGCA GCCATACATT CGGTTCTGTA GCTGCCAGCT CAATGGGGCA
    GCACTCATTC AGCAGAAAAC AGATGAAGTC CCCGAATTCA AGGAGTTTGT AAAAAGGTTA
    GCAATGGATC CTCGCTGTAA AGGAATGCCA CTATCAAGTT TTCTACTAAA GCCTATGCAA
    CGGGTAACAA GATACCCACT AATAATTAAA AATATAATAG AAAACACTCC TGAAAACCAT
    CCTGACCACA GTCACTTGAA GCATGCTCTT GAAAAAGCAG AAGAATTATG TTCTCAAGTA
    AATGAAGGAG TGCGAGAGAA AGAAAATTCA GATAGGCTGG AGTGGATCCA GGCTCATGTT
    CAGTGCGAAG GCCTCTCTGA GCAACTCGTA TTTAATTCTG TTACAAACTG CTTGGGACCA
    CGCAAGTTTC TGCACAGTGG GAAGCTCTAT AAAGCCAAGA GTAACAAGGA ACTGTATGGC
    TTTCTGTTCA ACGATTTCCT CCTCTTGACT CAGATCATAA AACCTCTTGG CTCTTCTGGC
    ACAGACAAGG TCTTCAGCCC CAAGTCTAAT CTGCAGTACA AAATGTACAA AACTCCCATT
    TTTCTAAATG AAGTACTAGT AAAATTACCC ACAGACCCTT CTGGAGATGA GCCCATTTTC
    CATATCTCCC ACATTGACAG AGTCTACACA CTCCGGGCTG AAAGCATTAA TGAAAGGACT
    GCGTGGGTTC AGAAAATTAA AGCTGCTTCG GAACTTTACA TAGAGACTGA AAAGAAGAAG
    AGGGAAAAGG CCTACTTAGT TCGCTCACAA AGAGCAACAG GCATCGGGAG GTTGATGGTG
    AATATTGTTG AAGGCATTGA ACTAAAACCT TGCAGATCCC ATGGAAAGAG TAATCCATAC
    TGTGAGGTGA CGATGGGCTC TCAGTGCCAC ATCACCAAGA CAATGCAGGA CACCCTCAAT
    CCAAAGTGGA ACTCCAACTG CCAGTTCTTT ATCAAAGACC TCGAGCAGGA CGTGCTCTGC
    ATCACGGTGT TTGAGAGGGA CCAGTTCTCC CCAGATGACT TTTTGGGCAG AACAGAGATC
    CGTGTGGCAG ACATTAAGAA GGATCAGGGT TCAAAGGGAC CAGTTACTAA GTGTCTCCTT
    CTCCATGAAG TCCCAACAGG AGAGATAGTC GTCCGACTAG ACCTGCAATT GTTTGATGAG
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015155489.1
    CDS213..5378
    Translation

    Target ORF information:

    RefSeq Version XM_015300003.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015300003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGA AAAGCTCCAC TAACAATTTC TGCTCAGGAG
    GATGTAAAAA TAGTGTATTA TAGAGCACTG TATCCTTTTG AATCAAGAAG CCATGACGAA
    ATCACTATTC AACCTGGCGA CATAGTCATG GTTAAAAGGG AATGGGTGGA TGAAAGTCAG
    ACTGGAGAAC CAGGGTGGCT TGGTGGAGAA TTGAAGGGAA AAACTGGATG GTTCCCAGCA
    AACTACGCAG AAAAAATACC TGAAAGTGAA GTTCCAGCAT CTATAAAACC AGTTGAGGCA
    GCTCCTGCAC CTAAAGTTTC TGTGCATGAA ACAACCACAT CACTAGGAAC TGCTGCATCT
    ACAGAGTGCA CGACAACTGC TAACAACTGG GCAGACTTCA GTTCAACATG GCCAGCAAAC
    ACTAATGAGA AACCAGAGAC AGACAACTGG GATGCCTGGG CAGCTCAACC ATCTTTGACT
    GTTCCCAGTG CAGGGCAGCT GCGGCAGCGA TCAGCCTTCA CCCCCGCCAC TGTTACAGGA
    TCGTCTCCCT CTCCTGTATT GGGTCAGGGT GAAAAAGTGG AGGGACTTCA AGCACAGGCT
    CTGTATCCTT GGAGAGCAAA AAAAGACAAC CACCTAAATT TCAACAAAAA TGATGTCATC
    ACAGTTTTAG AGCAGCAAGA TATGTGGTGG TTTGGAGAAG TTCAAGGACA AAAGGGGTGG
    TTTCCTAAGT CATATGTGAA GCTTATTTCA GGCCCCATTA GGAAGTCAAC AAGCATGGAT
    TCTGGTTCTT CAGAAAGTCC TGCTAGTCTG AAAAGAGTAG CATCACCAGC AGCTAAAGCA
    ACTATGTCAG GTGAAGAATA TGTTGCTATG TATACTTATG AGAGTTCTGA ACAAGGAGAC
    CTAACCTTTC AGCAGGGTGA TATGATTCTT GTGACAAAGA AAGATGGCGA CTGGTGGACA
    GGAACACTGG GTGATAAAAC AGGAGTTTTC CCATCAAATT ATGTGAGACT CAAAGATTCT
    GAGGCTTCTG GAGCAGCTGG AAAAACAGGA AGCTTAGGGA AGAAACCTGA AATTGCCCAA
    GTTATTGCCT CTTACACAGC AACGGGTCCA GAACAACTCA CCCTTGCTCC TGGTCAGTTG
    ATTCTTATCA GAAAGAAGAA CCCAGGTGGC TGGTGGGAAG GAGAGCTCCA AGCACGAGGG
    AAAAAACGAC AAATAGGATG GTTTCCTGCT AATTATGTGA AACTTTTGAG CCCTGGTACC
    AGTAAAACTA CACCAACCGA GCTACCTAAA TCAACAGCAT TACCTTCAGT GTGCCAAGTA
    ATTGGCATGT ATGACTACAC TGCACAGAAT GATGATGAGC TAGCATTCAA TAAAGGCCAG
    ATTATAAACG TCCTTAACAA GGAAGACCCA GACTGGTGGA AAGGGGAGGT GAATGGACAA
    GTGGGTCTCT TCCCATCCAA CTATGTGAAG CTGACAACAG ATATGGACCC AAGCCAGCAA
    TGGTGTGCAG ACTTGCATCT TCTGGATATG CTGACACCTA CTGAACGAAA AAGGCAGGGA
    TACATCCATG AGCTCATTGT CACAGAAGAG AACTACGTAA ATGATCTGCA GTTGGTCACA
    GAGATCTTCC AGAAACCACT AATGGAATCT GAGCTGCTAA CAGAAAAGGA AGTTGCTATG
    ATTTTTGTTA ATTGGAAGGA GCTGATTATG TGCAATATAA AACTACTGAA GGCCTTGCGT
    GTGCGTAAGA AGATGTCTGG GGAGAAGATG CCAGTGAAAA TGATTGGTGA CATCCTGACA
    GCTCAGCTGC CACACATGCA GCCATACATT CGGTTCTGTA GCTGCCAGCT CAATGGGGCA
    GCACTCATTC AGCAGAAAAC AGATGAAGTC CCCGAATTCA AGGAGTTTGT AAAAAGGTTA
    GCAATGGATC CTCGCTGTAA AGGAATGCCA CTATCAAGTT TTCTACTAAA GCCTATGCAA
    CGGGTAACAA GATACCCACT AATAATTAAA AATATAATAG AAAACACTCC TGAAAACCAT
    CCTGACCACA GTCACTTGAA GCATGCTCTT GAAAAAGCAG AAGAATTATG TTCTCAAGTA
    AATGAAGGAG TGCGAGAGAA AGAAAATTCA GATAGGCTGG AGTGGATCCA GGCTCATGTT
    CAGTGCGAAG GCCTCTCTGA GCAACTCGTA TTTAATTCTG TTACAAACTG CTTGGGACCA
    CGCAAGTTTC TGCACAGTGG GAAGCTCTAT AAAGCCAAGA GTAACAAGGA ACTGTATGGC
    TTTCTGTTCA ACGATTTCCT CCTCTTGACT CAGATCATAA AACCTCTTGG CTCTTCTGGC
    ACAGACAAGG TCTTCAGCCC CAAGTCTAAT CTGCAGTACA AAATGTACAA AACTCCCATT
    TTTCTAAATG AAGTACTAGT AAAATTACCC ACAGACCCTT CTGGAGATGA GCCCATTTTC
    CATATCTCCC ACATTGACAG AGTCTACACA CTCCGGGCTG AAAGCATTAA TGAAAGGACT
    GCGTGGGTTC AGAAAATTAA AGCTGCTTCG GAACTTTACA TAGAGACTGA AAAGAAGAAG
    AGGGAAAAGG CCTACTTAGT TCGCTCACAA AGAGCAACAG GCATCGGGAG GTTGATGGTG
    AATATTGTTG AAGGCATTGA ACTAAAACCT TGCAGATCCC ATGGAAAGAG TAATCCATAC
    TGTGAGGTGA CGATGGGCTC TCAGTGCCAC ATCACCAAGA CAATGCAGGA CACCCTCAAT
    CCAAAGTGGA ACTCCAACTG CCAGTTCTTT ATCAAAGACC TCGAGCAGGA CGTGCTCTGC
    ATCACGGTGT TTGAGAGGGA CCAGTTCTCC CCAGATGACT TTTTGGGCAG AACAGAGATC
    CGTGTGGCAG ACATTAAGAA GGATCAGGGT TCAAAGGGAC CAGTTACTAA GTGTCTCCTT
    CTCCATGAAG TCCCAACAGG AGAGATAGTC GTCCGACTAG ACCTGCAATT GTTTGATGAG
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30287
    Clone ID Related Accession (Same CDS sequence) XM_015300017.2 , XM_015300017.1
    Accession Version XM_015300017.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5052bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product intersectin-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015300017.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGT AAGCTTTTCC AACCACATCA AGAACCAGTT
    AAGCCAGCTG TCCAGGCTCC ATGGTCAAAT GCAGGAAAAG CTCCACTAAC AATTTCTGCT
    CAGGAGGATG TAAAAATAGT GTATTATAGA GCACTGTATC CTTTTGAATC AAGAAGCCAT
    GACGAAATCA CTATTCAACC TGGCGACATA GTCATGGTTA AAAGGGAATG GGTGGATGAA
    AGTCAGACTG GAGAACCAGG GTGGCTTGGT GGAGAATTGA AGGGAAAAAC TGGATGGTTC
    CCAGCAAACT ACGCAGAAAA AATACCTGAA AGTGAAGTTC CAGCATCTAT AAAACCAGTT
    GAGGCAGCTC CTGCACCTAA AGTTTCTGTG CATGAAACAA CCACATCACT AGGAACTGCT
    GCATCTACAG AGTGCACGAC AACTGCTAAC AACTGGGCAG ACTTCAGTTC AACATGGCCA
    GCAAACACTA ATGAGAAACC AGAGACAGAC AACTGGGATG CCTGGGCAGC TCAACCATCT
    TTGACTGTTC CCAGTGCAGG GCAGCTGCGG CAGCGATCAG CCTTCACCCC CGCCACTGTT
    ACAGGATCGT CTCCCTCTCC TGTATTGGGT CAGGGTGAAA AAGTGGAGGG ACTTCAAGCA
    CAGGCTCTGT ATCCTTGGAG AGCAAAAAAA GACAACCACC TAAATTTCAA CAAAAATGAT
    GTCATCACAG TTTTAGAGCA GCAAGATATG TGGTGGTTTG GAGAAGTTCA AGGACAAAAG
    GGGTGGTTTC CTAAGTCATA TGTGAAGCTT ATTTCAGGCC CCATTAGGAA GTCAACAAGC
    ATGGATTCTG GTTCTTCAGA AAGTCCTGCT AGTCTGAAAA GAGTAGCATC ACCAGCAGCT
    AAAGCAACTA TGTCAGGTGA AGAATATGTT GCTATGTATA CTTATGAGAG TTCTGAACAA
    GGAGACCTAA CCTTTCAGCA GGGTGATATG ATTCTTGTGA CAAAGAAAGA TGGCGACTGG
    TGGACAGGAA CACTGGGTGA TAAAACAGGA GTTTTCCCAT CAAATTATGT GAGACTCAAA
    GATTCTGAGG CTTCTGGAGC AGCTGGAAAA ACAGGAAGCT TAGGGAAGAA ACCTGAAATT
    GCCCAAGTTA TTGCCTCTTA CACAGCAACG GGTCCAGAAC AACTCACCCT TGCTCCTGGT
    CAGTTGATTC TTATCAGAAA GAAGAACCCA GGTGGCTGGT GGGAAGGAGA GCTCCAAGCA
    CGAGGGAAAA AACGACAAAT AGGATGGTTT CCTGCTAATT ATGTGAAACT TTTGAGCCCT
    GGTACCAGTA AAACTACACC AACCGAGCTA CCTAAATCAA CAGCATTACC TTCAGTGTGC
    CAAGTAATTG GCATGTATGA CTACACTGCA CAGAATGATG ATGAGCTAGC ATTCAATAAA
    GGCCAGATTA TAAACGTCCT TAACAAGGAA GACCCAGACT GGTGGAAAGG GGAGGTGAAT
    GGACAAGTGG GTCTCTTCCC ATCCAACTAT GTGAAGCTGA CAACAGATAT GGACCCAAGC
    CAGCAATGGT GTGCAGACTT GCATCTTCTG GATATGCTGA CACCTACTGA ACGAAAAAGG
    CAGGGATACA TCCATGAGCT CATTGTCACA GAAGAGAACT ACGTAAATGA TCTGCAGTTG
    GTCACAGAGA TCTTCCAGAA ACCACTAATG GAATCTGAGC TGCTAACAGA AAAGGAAGTT
    GCTATGATTT TTGTTAATTG GAAGGAGCTG ATTATGTGCA ATATAAAACT ACTGAAGGCC
    TTGCGTGTGC GTAAGAAGAT GTCTGGGGAG AAGATGCCAG TGAAAATGAT TGGTGACATC
    CTGACAGCTC AGCTGCCACA CATGCAGCCA TACATTCGGT TCTGTAGCTG CCAGCTCAAT
    GGGGCAGCAC TCATTCAGCA GAAAACAGAT GAAGTCCCCG AATTCAAGGA GTTTGTAAAA
    AGGTTAGCAA TGGATCCTCG CTGTAAAGGA ATGCCACTAT CAAGTTTTCT ACTAAAGCCT
    ATGCAACGGG TAACAAGATA CCCACTAATA ATTAAAAATA TAATAGAAAA CACTCCTGAA
    AACCATCCTG ACCACAGTCA CTTGAAGCAT GCTCTTGAAA AAGCAGAAGA ATTATGTTCT
    CAAGTAAATG AAGGAGTGCG AGAGAAAGAA AATTCAGATA GGCTGGAGTG GATCCAGGCT
    CATGTTCAGT GCGAAGGCCT CTCTGAGCAA CTCGTATTTA ATTCTGTTAC AAACTGCTTG
    GGACCACGCA AGTTTCTGCA CAGTGGGAAG CTCTATAAAG CCAAGAGTAA CAAGGAACTG
    TATGGCTTTC TGTTCAACGA TTTCCTCCTC TTGACTCAGA TCATAAAACC TCTTGGCTCT
    TCTGGCACAG ACAAGGTCTT CAGCCCCAAG TCTAATCTGC AGTACAAAAT GTACAAAACT
    CCCATTTTTC TAAATGAAGT ACTAGTAAAA TTACCCACAG ACCCTTCTGG AGATGAGCCC
    ATTTTCCATA TCTCCCACAT TGACAGAGTC TACACACTCC GGGCTGAAAG CATTAATGAA
    AGGACTGCGT GGGTTCAGAA AATTAAAGCT GCTTCGGAAC TTTACATAGA GACTGAAAAG
    AAGAAGAGGG AAAAGGCCTA CTTAGTTCGC TCACAAAGAG CAACAGGCAT CGGGAGGTTG
    ATGGTGAATA TTGTTGAAGG CATTGAACTA AAACCTTGCA GATCCCATGG AAAGAGTAAT
    CCATACTGTG AGGTGACGAT GGGCTCTCAG TGCCACATCA CCAAGACAAT GCAGGACACC
    CTCAATCCAA AGTGGAACTC CAACTGCCAG TTCTTTATCA AAGACCTCGA GCAGGACGTG
    CTCTGCATCA CGGTGTTTGA GAGGGACCAG TTCTCCCCAG ATGACTTTTT GGGCAGAACA
    GAGATCCGTG TGGCAGACAT TAAGAAGGAT CAGGGTTCAA AGGGACCAGT TACTAAGTGT
    CTCCTTCTCC ATGAAGTCCC AACAGGAGAG ATAGTCGTCC GACTAGACCT GCAATTGTTT
    GATGAGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015155503.1
    CDS231..5282
    Translation

    Target ORF information:

    RefSeq Version XM_015300017.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus intersectin 1 (ITSN1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015300017.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGT AAGCTTTTCC AACCACATCA AGAACCAGTT
    AAGCCAGCTG TCCAGGCTCC ATGGTCAAAT GCAGGAAAAG CTCCACTAAC AATTTCTGCT
    CAGGAGGATG TAAAAATAGT GTATTATAGA GCACTGTATC CTTTTGAATC AAGAAGCCAT
    GACGAAATCA CTATTCAACC TGGCGACATA GTCATGGTTA AAAGGGAATG GGTGGATGAA
    AGTCAGACTG GAGAACCAGG GTGGCTTGGT GGAGAATTGA AGGGAAAAAC TGGATGGTTC
    CCAGCAAACT ACGCAGAAAA AATACCTGAA AGTGAAGTTC CAGCATCTAT AAAACCAGTT
    GAGGCAGCTC CTGCACCTAA AGTTTCTGTG CATGAAACAA CCACATCACT AGGAACTGCT
    GCATCTACAG AGTGCACGAC AACTGCTAAC AACTGGGCAG ACTTCAGTTC AACATGGCCA
    GCAAACACTA ATGAGAAACC AGAGACAGAC AACTGGGATG CCTGGGCAGC TCAACCATCT
    TTGACTGTTC CCAGTGCAGG GCAGCTGCGG CAGCGATCAG CCTTCACCCC CGCCACTGTT
    ACAGGATCGT CTCCCTCTCC TGTATTGGGT CAGGGTGAAA AAGTGGAGGG ACTTCAAGCA
    CAGGCTCTGT ATCCTTGGAG AGCAAAAAAA GACAACCACC TAAATTTCAA CAAAAATGAT
    GTCATCACAG TTTTAGAGCA GCAAGATATG TGGTGGTTTG GAGAAGTTCA AGGACAAAAG
    GGGTGGTTTC CTAAGTCATA TGTGAAGCTT ATTTCAGGCC CCATTAGGAA GTCAACAAGC
    ATGGATTCTG GTTCTTCAGA AAGTCCTGCT AGTCTGAAAA GAGTAGCATC ACCAGCAGCT
    AAAGCAACTA TGTCAGGTGA AGAATATGTT GCTATGTATA CTTATGAGAG TTCTGAACAA
    GGAGACCTAA CCTTTCAGCA GGGTGATATG ATTCTTGTGA CAAAGAAAGA TGGCGACTGG
    TGGACAGGAA CACTGGGTGA TAAAACAGGA GTTTTCCCAT CAAATTATGT GAGACTCAAA
    GATTCTGAGG CTTCTGGAGC AGCTGGAAAA ACAGGAAGCT TAGGGAAGAA ACCTGAAATT
    GCCCAAGTTA TTGCCTCTTA CACAGCAACG GGTCCAGAAC AACTCACCCT TGCTCCTGGT
    CAGTTGATTC TTATCAGAAA GAAGAACCCA GGTGGCTGGT GGGAAGGAGA GCTCCAAGCA
    CGAGGGAAAA AACGACAAAT AGGATGGTTT CCTGCTAATT ATGTGAAACT TTTGAGCCCT
    GGTACCAGTA AAACTACACC AACCGAGCTA CCTAAATCAA CAGCATTACC TTCAGTGTGC
    CAAGTAATTG GCATGTATGA CTACACTGCA CAGAATGATG ATGAGCTAGC ATTCAATAAA
    GGCCAGATTA TAAACGTCCT TAACAAGGAA GACCCAGACT GGTGGAAAGG GGAGGTGAAT
    GGACAAGTGG GTCTCTTCCC ATCCAACTAT GTGAAGCTGA CAACAGATAT GGACCCAAGC
    CAGCAATGGT GTGCAGACTT GCATCTTCTG GATATGCTGA CACCTACTGA ACGAAAAAGG
    CAGGGATACA TCCATGAGCT CATTGTCACA GAAGAGAACT ACGTAAATGA TCTGCAGTTG
    GTCACAGAGA TCTTCCAGAA ACCACTAATG GAATCTGAGC TGCTAACAGA AAAGGAAGTT
    GCTATGATTT TTGTTAATTG GAAGGAGCTG ATTATGTGCA ATATAAAACT ACTGAAGGCC
    TTGCGTGTGC GTAAGAAGAT GTCTGGGGAG AAGATGCCAG TGAAAATGAT TGGTGACATC
    CTGACAGCTC AGCTGCCACA CATGCAGCCA TACATTCGGT TCTGTAGCTG CCAGCTCAAT
    GGGGCAGCAC TCATTCAGCA GAAAACAGAT GAAGTCCCCG AATTCAAGGA GTTTGTAAAA
    AGGTTAGCAA TGGATCCTCG CTGTAAAGGA ATGCCACTAT CAAGTTTTCT ACTAAAGCCT
    ATGCAACGGG TAACAAGATA CCCACTAATA ATTAAAAATA TAATAGAAAA CACTCCTGAA
    AACCATCCTG ACCACAGTCA CTTGAAGCAT GCTCTTGAAA AAGCAGAAGA ATTATGTTCT
    CAAGTAAATG AAGGAGTGCG AGAGAAAGAA AATTCAGATA GGCTGGAGTG GATCCAGGCT
    CATGTTCAGT GCGAAGGCCT CTCTGAGCAA CTCGTATTTA ATTCTGTTAC AAACTGCTTG
    GGACCACGCA AGTTTCTGCA CAGTGGGAAG CTCTATAAAG CCAAGAGTAA CAAGGAACTG
    TATGGCTTTC TGTTCAACGA TTTCCTCCTC TTGACTCAGA TCATAAAACC TCTTGGCTCT
    TCTGGCACAG ACAAGGTCTT CAGCCCCAAG TCTAATCTGC AGTACAAAAT GTACAAAACT
    CCCATTTTTC TAAATGAAGT ACTAGTAAAA TTACCCACAG ACCCTTCTGG AGATGAGCCC
    ATTTTCCATA TCTCCCACAT TGACAGAGTC TACACACTCC GGGCTGAAAG CATTAATGAA
    AGGACTGCGT GGGTTCAGAA AATTAAAGCT GCTTCGGAAC TTTACATAGA GACTGAAAAG
    AAGAAGAGGG AAAAGGCCTA CTTAGTTCGC TCACAAAGAG CAACAGGCAT CGGGAGGTTG
    ATGGTGAATA TTGTTGAAGG CATTGAACTA AAACCTTGCA GATCCCATGG AAAGAGTAAT
    CCATACTGTG AGGTGACGAT GGGCTCTCAG TGCCACATCA CCAAGACAAT GCAGGACACC
    CTCAATCCAA AGTGGAACTC CAACTGCCAG TTCTTTATCA AAGACCTCGA GCAGGACGTG
    CTCTGCATCA CGGTGTTTGA GAGGGACCAG TTCTCCCCAG ATGACTTTTT GGGCAGAACA
    GAGATCCGTG TGGCAGACAT TAAGAAGGAT CAGGGTTCAA AGGGACCAGT TACTAAGTGT
    CTCCTTCTCC ATGAAGTCCC AACAGGAGAG ATAGTCGTCC GACTAGACCT GCAATTGTTT
    GATGAGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30287
    Clone ID Related Accession (Same CDS sequence) XM_015300017.2 , XM_015300017.1
    Accession Version XM_015300017.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5052bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product intersectin-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455737.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGT AAGCTTTTCC AACCACATCA AGAACCAGTT
    AAGCCAGCTG TCCAGGCTCC ATGGTCAAAT GCAGGAAAAG CTCCACTAAC AATTTCTGCT
    CAGGAGGATG TAAAAATAGT GTATTATAGA GCACTGTATC CTTTTGAATC AAGAAGCCAT
    GACGAAATCA CTATTCAACC TGGCGACATA GTCATGGTTA AAAGGGAATG GGTGGATGAA
    AGTCAGACTG GAGAACCAGG GTGGCTTGGT GGAGAATTGA AGGGAAAAAC TGGATGGTTC
    CCAGCAAACT ACGCAGAAAA AATACCTGAA AGTGAAGTTC CAGCATCTAT AAAACCAGTT
    GAGGCAGCTC CTGCACCTAA AGTTTCTGTG CATGAAACAA CCACATCACT AGGAACTGCT
    GCATCTACAG AGTGCACGAC AACTGCTAAC AACTGGGCAG ACTTCAGTTC AACATGGCCA
    GCAAACACTA ATGAGAAACC AGAGACAGAC AACTGGGATG CCTGGGCAGC TCAACCATCT
    TTGACTGTTC CCAGTGCAGG GCAGCTGCGG CAGCGATCAG CCTTCACCCC CGCCACTGTT
    ACAGGATCGT CTCCCTCTCC TGTATTGGGT CAGGGTGAAA AAGTGGAGGG ACTTCAAGCA
    CAGGCTCTGT ATCCTTGGAG AGCAAAAAAA GACAACCACC TAAATTTCAA CAAAAATGAT
    GTCATCACAG TTTTAGAGCA GCAAGATATG TGGTGGTTTG GAGAAGTTCA AGGACAAAAG
    GGGTGGTTTC CTAAGTCATA TGTGAAGCTT ATTTCAGGCC CCATTAGGAA GTCAACAAGC
    ATGGATTCTG GTTCTTCAGA AAGTCCTGCT AGTCTGAAAA GAGTAGCATC ACCAGCAGCT
    AAAGCAACTA TGTCAGGTGA AGAATATGTT GCTATGTATA CTTATGAGAG TTCTGAACAA
    GGAGACCTAA CCTTTCAGCA GGGTGATATG ATTCTTGTGA CAAAGAAAGA TGGCGACTGG
    TGGACAGGAA CACTGGGTGA TAAAACAGGA GTTTTCCCAT CAAATTATGT GAGACTCAAA
    GATTCTGAGG CTTCTGGAGC AGCTGGAAAA ACAGGAAGCT TAGGGAAGAA ACCTGAAATT
    GCCCAAGTTA TTGCCTCTTA CACAGCAACG GGTCCAGAAC AACTCACCCT TGCTCCTGGT
    CAGTTGATTC TTATCAGAAA GAAGAACCCA GGTGGCTGGT GGGAAGGAGA GCTCCAAGCA
    CGAGGGAAAA AACGACAAAT AGGATGGTTT CCTGCTAATT ATGTGAAACT TTTGAGCCCT
    GGTACCAGTA AAACTACACC AACCGAGCTA CCTAAATCAA CAGCATTACC TTCAGTGTGC
    CAAGTAATTG GCATGTATGA CTACACTGCA CAGAATGATG ATGAGCTAGC ATTCAATAAA
    GGCCAGATTA TAAACGTCCT TAACAAGGAA GACCCAGACT GGTGGAAAGG GGAGGTGAAT
    GGACAAGTGG GTCTCTTCCC ATCCAACTAT GTGAAGCTGA CAACAGATAT GGACCCAAGC
    CAGCAATGGT GTGCAGACTT GCATCTTCTG GATATGCTGA CACCTACTGA ACGAAAAAGG
    CAGGGATACA TCCATGAGCT CATTGTCACA GAAGAGAACT ACGTAAATGA TCTGCAGTTG
    GTCACAGAGA TCTTCCAGAA ACCACTAATG GAATCTGAGC TGCTAACAGA AAAGGAAGTT
    GCTATGATTT TTGTTAATTG GAAGGAGCTG ATTATGTGCA ATATAAAACT ACTGAAGGCC
    TTGCGTGTGC GTAAGAAGAT GTCTGGGGAG AAGATGCCAG TGAAAATGAT TGGTGACATC
    CTGACAGCTC AGCTGCCACA CATGCAGCCA TACATTCGGT TCTGTAGCTG CCAGCTCAAT
    GGGGCAGCAC TCATTCAGCA GAAAACAGAT GAAGTCCCCG AATTCAAGGA GTTTGTAAAA
    AGGTTAGCAA TGGATCCTCG CTGTAAAGGA ATGCCACTAT CAAGTTTTCT ACTAAAGCCT
    ATGCAACGGG TAACAAGATA CCCACTAATA ATTAAAAATA TAATAGAAAA CACTCCTGAA
    AACCATCCTG ACCACAGTCA CTTGAAGCAT GCTCTTGAAA AAGCAGAAGA ATTATGTTCT
    CAAGTAAATG AAGGAGTGCG AGAGAAAGAA AATTCAGATA GGCTGGAGTG GATCCAGGCT
    CATGTTCAGT GCGAAGGCCT CTCTGAGCAA CTCGTATTTA ATTCTGTTAC AAACTGCTTG
    GGACCACGCA AGTTTCTGCA CAGTGGGAAG CTCTATAAAG CCAAGAGTAA CAAGGAACTG
    TATGGCTTTC TGTTCAACGA TTTCCTCCTC TTGACTCAGA TCATAAAACC TCTTGGCTCT
    TCTGGCACAG ACAAGGTCTT CAGCCCCAAG TCTAATCTGC AGTACAAAAT GTACAAAACT
    CCCATTTTTC TAAATGAAGT ACTAGTAAAA TTACCCACAG ACCCTTCTGG AGATGAGCCC
    ATTTTCCATA TCTCCCACAT TGACAGAGTC TACACACTCC GGGCTGAAAG CATTAATGAA
    AGGACTGCGT GGGTTCAGAA AATTAAAGCT GCTTCGGAAC TTTACATAGA GACTGAAAAG
    AAGAAGAGGG AAAAGGCCTA CTTAGTTCGC TCACAAAGAG CAACAGGCAT CGGGAGGTTG
    ATGGTGAATA TTGTTGAAGG CATTGAACTA AAACCTTGCA GATCCCATGG AAAGAGTAAT
    CCATACTGTG AGGTGACGAT GGGCTCTCAG TGCCACATCA CCAAGACAAT GCAGGACACC
    CTCAATCCAA AGTGGAACTC CAACTGCCAG TTCTTTATCA AAGACCTCGA GCAGGACGTG
    CTCTGCATCA CGGTGTTTGA GAGGGACCAG TTCTCCCCAG ATGACTTTTT GGGCAGAACA
    GAGATCCGTG TGGCAGACAT TAAGAAGGAT CAGGGTTCAA AGGGACCAGT TACTAAGTGT
    CTCCTTCTCC ATGAAGTCCC AACAGGAGAG ATAGTCGTCC GACTAGACCT GCAATTGTTT
    GATGAGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015155503.1
    CDS213..5264
    Translation

    Target ORF information:

    RefSeq Version XM_015300017.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015300017.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GTCAATGGCA ATGGAAGATG CAATGCTGCA GTGCCTGGCA
    GTTCTCCTGG GCTGCGTCAA CTACTTAGAC TCCTTTCTAA AGGAGCTGAG AGAAATACAT
    AACAGACAGC AGCTTCAGAA ACAAAAGAAC TTGGAAGCTG AGAGGCTGAA ACAGAAAGAA
    CAAGAAAGGA AGACAGAACT GGAAAAGCAA AAAGAAGCTC AAAGGCGAAT CCAGGATCGG
    GATAAACAAC GGCTTGATCG AGTGCAACAA GAAGAAGAAC CTCAATGGCA AAAAAAAAAT
    CAAGAAGATG ACAAGCAAAA AAGGGAAGAA ATAATCAAGA AGAAGGAAAG TGAGGATAAG
    GGGAAACAGG AAATCCAAGA GAAACCGAGT AAGCTTTTCC AACCACATCA AGAACCAGTT
    AAGCCAGCTG TCCAGGCTCC ATGGTCAAAT GCAGGAAAAG CTCCACTAAC AATTTCTGCT
    CAGGAGGATG TAAAAATAGT GTATTATAGA GCACTGTATC CTTTTGAATC AAGAAGCCAT
    GACGAAATCA CTATTCAACC TGGCGACATA GTCATGGTTA AAAGGGAATG GGTGGATGAA
    AGTCAGACTG GAGAACCAGG GTGGCTTGGT GGAGAATTGA AGGGAAAAAC TGGATGGTTC
    CCAGCAAACT ACGCAGAAAA AATACCTGAA AGTGAAGTTC CAGCATCTAT AAAACCAGTT
    GAGGCAGCTC CTGCACCTAA AGTTTCTGTG CATGAAACAA CCACATCACT AGGAACTGCT
    GCATCTACAG AGTGCACGAC AACTGCTAAC AACTGGGCAG ACTTCAGTTC AACATGGCCA
    GCAAACACTA ATGAGAAACC AGAGACAGAC AACTGGGATG CCTGGGCAGC TCAACCATCT
    TTGACTGTTC CCAGTGCAGG GCAGCTGCGG CAGCGATCAG CCTTCACCCC CGCCACTGTT
    ACAGGATCGT CTCCCTCTCC TGTATTGGGT CAGGGTGAAA AAGTGGAGGG ACTTCAAGCA
    CAGGCTCTGT ATCCTTGGAG AGCAAAAAAA GACAACCACC TAAATTTCAA CAAAAATGAT
    GTCATCACAG TTTTAGAGCA GCAAGATATG TGGTGGTTTG GAGAAGTTCA AGGACAAAAG
    GGGTGGTTTC CTAAGTCATA TGTGAAGCTT ATTTCAGGCC CCATTAGGAA GTCAACAAGC
    ATGGATTCTG GTTCTTCAGA AAGTCCTGCT AGTCTGAAAA GAGTAGCATC ACCAGCAGCT
    AAAGCAACTA TGTCAGGTGA AGAATATGTT GCTATGTATA CTTATGAGAG TTCTGAACAA
    GGAGACCTAA CCTTTCAGCA GGGTGATATG ATTCTTGTGA CAAAGAAAGA TGGCGACTGG
    TGGACAGGAA CACTGGGTGA TAAAACAGGA GTTTTCCCAT CAAATTATGT GAGACTCAAA
    GATTCTGAGG CTTCTGGAGC AGCTGGAAAA ACAGGAAGCT TAGGGAAGAA ACCTGAAATT
    GCCCAAGTTA TTGCCTCTTA CACAGCAACG GGTCCAGAAC AACTCACCCT TGCTCCTGGT
    CAGTTGATTC TTATCAGAAA GAAGAACCCA GGTGGCTGGT GGGAAGGAGA GCTCCAAGCA
    CGAGGGAAAA AACGACAAAT AGGATGGTTT CCTGCTAATT ATGTGAAACT TTTGAGCCCT
    GGTACCAGTA AAACTACACC AACCGAGCTA CCTAAATCAA CAGCATTACC TTCAGTGTGC
    CAAGTAATTG GCATGTATGA CTACACTGCA CAGAATGATG ATGAGCTAGC ATTCAATAAA
    GGCCAGATTA TAAACGTCCT TAACAAGGAA GACCCAGACT GGTGGAAAGG GGAGGTGAAT
    GGACAAGTGG GTCTCTTCCC ATCCAACTAT GTGAAGCTGA CAACAGATAT GGACCCAAGC
    CAGCAATGGT GTGCAGACTT GCATCTTCTG GATATGCTGA CACCTACTGA ACGAAAAAGG
    CAGGGATACA TCCATGAGCT CATTGTCACA GAAGAGAACT ACGTAAATGA TCTGCAGTTG
    GTCACAGAGA TCTTCCAGAA ACCACTAATG GAATCTGAGC TGCTAACAGA AAAGGAAGTT
    GCTATGATTT TTGTTAATTG GAAGGAGCTG ATTATGTGCA ATATAAAACT ACTGAAGGCC
    TTGCGTGTGC GTAAGAAGAT GTCTGGGGAG AAGATGCCAG TGAAAATGAT TGGTGACATC
    CTGACAGCTC AGCTGCCACA CATGCAGCCA TACATTCGGT TCTGTAGCTG CCAGCTCAAT
    GGGGCAGCAC TCATTCAGCA GAAAACAGAT GAAGTCCCCG AATTCAAGGA GTTTGTAAAA
    AGGTTAGCAA TGGATCCTCG CTGTAAAGGA ATGCCACTAT CAAGTTTTCT ACTAAAGCCT
    ATGCAACGGG TAACAAGATA CCCACTAATA ATTAAAAATA TAATAGAAAA CACTCCTGAA
    AACCATCCTG ACCACAGTCA CTTGAAGCAT GCTCTTGAAA AAGCAGAAGA ATTATGTTCT
    CAAGTAAATG AAGGAGTGCG AGAGAAAGAA AATTCAGATA GGCTGGAGTG GATCCAGGCT
    CATGTTCAGT GCGAAGGCCT CTCTGAGCAA CTCGTATTTA ATTCTGTTAC AAACTGCTTG
    GGACCACGCA AGTTTCTGCA CAGTGGGAAG CTCTATAAAG CCAAGAGTAA CAAGGAACTG
    TATGGCTTTC TGTTCAACGA TTTCCTCCTC TTGACTCAGA TCATAAAACC TCTTGGCTCT
    TCTGGCACAG ACAAGGTCTT CAGCCCCAAG TCTAATCTGC AGTACAAAAT GTACAAAACT
    CCCATTTTTC TAAATGAAGT ACTAGTAAAA TTACCCACAG ACCCTTCTGG AGATGAGCCC
    ATTTTCCATA TCTCCCACAT TGACAGAGTC TACACACTCC GGGCTGAAAG CATTAATGAA
    AGGACTGCGT GGGTTCAGAA AATTAAAGCT GCTTCGGAAC TTTACATAGA GACTGAAAAG
    AAGAAGAGGG AAAAGGCCTA CTTAGTTCGC TCACAAAGAG CAACAGGCAT CGGGAGGTTG
    ATGGTGAATA TTGTTGAAGG CATTGAACTA AAACCTTGCA GATCCCATGG AAAGAGTAAT
    CCATACTGTG AGGTGACGAT GGGCTCTCAG TGCCACATCA CCAAGACAAT GCAGGACACC
    CTCAATCCAA AGTGGAACTC CAACTGCCAG TTCTTTATCA AAGACCTCGA GCAGGACGTG
    CTCTGCATCA CGGTGTTTGA GAGGGACCAG TTCTCCCCAG ATGACTTTTT GGGCAGAACA
    GAGATCCGTG TGGCAGACAT TAAGAAGGAT CAGGGTTCAA AGGGACCAGT TACTAAGTGT
    CTCCTTCTCC ATGAAGTCCC AACAGGAGAG ATAGTCGTCC GACTAGACCT GCAATTGTTT
    GATGAGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30288
    Clone ID Related Accession (Same CDS sequence) XM_015300022.1 , XM_015300022.2
    Accession Version XM_015300022.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product intersectin-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455737.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAACAATG ATGGAAGGAT GGATCAGCTG GAGTTTTCAA TAGCTATGAA ACTTATCAAA 
    TTAAAACTAC AAGGCTATCA ACTCCCATCT GCGCTGCCTC CTGTCATGAA GCAGCCACCT
    ATTGCTCTAC CTAGTGCACC AGGGTTTGGT ATTGGGGGCA TTGCCAGCAT GCCATCTCTT
    ACAACTGTTG CCCCAGTGCC AATGGCATCT ATTCCAGTAG TAGGAATGTC TCCGCCATTA
    GTATCTTCTG TTCCTGCAGC AGCAGTGCCT CCCCTGGCTA ATGGAGCTCC TGCTGTTATA
    CAGCCTCTGC CTGCTTTTGC TCATCCTGCC ACATTGCCAA AGAGTTCTTC CTTTAGTAGA
    TCTGGTCCAG GATCGCAGCT GAACACAAAA CTGCAAAAGG CACAATCGTT TGACGTGGCG
    AGTGTGCCTC CTGTGGCAGA GTGGGCAGTA CCTCAGTCAT CAAGACTGAA ATACAGACAG
    TTGTTCAATA GCCACGATAA AACAATGAGT GGACACTTAA CAGGTCCGCA AGCAAGAACT
    ATTCTTATGC AGTCAAGTTT ACCCCAGGCT CAGCTGGCTA CAATATGGAA TCTTTCAGAT
    ATTGATCAAG ATGGAAAGCT TACAGCTGAA GAGTTTATTC TGGCTATGCA CTTAATTGAT
    GTAGCTATGT CTGGTCAGCC ACTGCCACCT GTACTGCCTC CAGAGTATAT TCCACCTTCA
    TTTAGAAGAG TACGCTCTGG TAGTGGTGTA TCTGCTGTAA GTTCAGTATC TGTAGACCAA
    AGGTTACCAG AAGAACCAGC ATTAGAAGAA GAACAGCAGC AACTGGAAAA GAAATTGCCA
    GTTACATTTG AAGATAAAAA ACGTGAGAAC TTTGAACGTG GCAATCTAGA ACTTGAAAAA
    CGGAGGCAGG CTCTCCTGGA ACAGCAACGC AAAGAGCAAG AGCGTCTAGC ACAGCTGGAA
    CGGGCAGAGC AAGAAAGGAA GGAACGTGAA CGACAAGAGC AAGAAAGGAA AAGACAGCTG
    GAGCTAGAGA AACAGTTGGA GAAACAACGG GAATTGGAAC GGCAGAGAGA AGAGGAAAGG
    AGGAAAGAAA TAGAAAGAAG AGAGGCTGCA AAACGGGAAC TTGAGAGGCA AAGGCAACTT
    GAATGGGAAC GTAATCGTCG GCAAGAACTG CTAAATCAAA GAAACAGAGA ACAAGAGGAC
    ATCGTTGTTC TGAAGGCAAA GAAGAAGACT TTAGAATTTG AGCTGGAAGC TCTAAATGAT
    AAAAAAAATC AGTTGGAAGG AAAGCTTCAG GATATCAGAT GTCGGCTGTC TACCCAAAGA
    CAAGAAATTG AAAGTACAAA TAAATCTAGA GAACTGAGAA TTGCAGAAAT CACCCATTTG
    CAACAGCAGC TACAGGAGTC TCAGCAGATG CTTGGAAAGT TGATTCCAGA AAAGCAATTA
    CTCAATGACC AACTAAAGCA GGTTCAACAG AACAGTTTGC ATAGAGATTC TCTTCTTACT
    ATCAAAAGAG CCTTAGAAGC CAAGGAACTA GCCCGTCAAC AGCTTCGAGA CCAGCTAGAT
    GAAGTAGAAA AAGAAACCAG ATCTAAACTT CAGGAAATTG ATATTTTCAA TAATCAGCTG
    AAGTCAATGG CAATGGAAGA TGCAATGCTG CAGTGCCTGG CAGTTCTCCT GGGCTGCGTC
    AACTACTTAG ACTCCTTTCT AAAGGAGCTG AGAGAAATAC ATAACAGACA GCAGCTTCAG
    AAACAAAAGA ACTTGGAAGC TGAGAGGCTG AAACAGAAAG AACAAGAAAG GAAGACAGAA
    CTGGAAAAGC AAAAAGAAGC TCAAAGGCGA ATCCAGGATC GGGATAAACA ACGGCTTGAT
    CGAGTGCAAC AAGAAGAAGA ACCTCAATGG CAAAAAAAAA ATCAAGAAGA TGACAAGCAA
    AAAAGGGAAG AAATAATCAA GAAGAAGGAA AGTGAGGATA AGGGGAAACA GGAAATCCAA
    GAGAAACCGA GTAAGCTTTT CCAACCACAT CAAGAACCAG TTAAGCCAGC TGTCCAGGCT
    CCATGGTCAA ATGCAGGAAA AGCTCCACTA ACAATTTCTG CTCAGGAGGA TGTAAAAATA
    GTGTATTATA GAGCACTGTA TCCTTTTGAA TCAAGAAGCC ATGACGAAAT CACTATTCAA
    CCTGGCGACA TAGTCATGGT TAAAAGGGAA TGGGTGGATG AAAGTCAGAC TGGAGAACCA
    GGGTGGCTTG GTGGAGAATT GAAGGGAAAA ACTGGATGGT TCCCAGCAAA CTACGCAGAA
    AAAATACCTG AAAGTGAAGT TCCAGCATCT ATAAAACCAG TTGAGGCAGC TCCTGCACCT
    AAAGTTTCTG TGCATGAAAC AACCACATCA CTAGGAACTG CTGCATCTAC AGAGTGCACG
    ACAACTGCTA ACAACTGGGC AGACTTCAGT TCAACATGGC CAGCAAACAC TAATGAGAAA
    CCAGAGACAG ACAACTGGGA TGCCTGGGCA GCTCAACCAT CTTTGACTGT TCCCAGTGCA
    GGGCAGCTGC GGCAGCGATC AGCCTTCACC CCCGCCACTG TTACAGGATC GTCTCCCTCT
    CCTGTATTGG GTCAGGGTGA AAAAGTGGAG GGACTTCAAG CACAGGCTCT GTATCCTTGG
    AGAGCAAAAA AAGACAACCA CCTAAATTTC AACAAAAATG ATGTCATCAC AGTTTTAGAG
    CAGCAAGATA TGTGGTGGTT TGGAGAAGTT CAAGGACAAA AGGGGTGGTT TCCTAAGTCA
    TATGTGAAGC TTATTTCAGG CCCCATTAGG AAGTCAACAA GCATGGATTC TGGTTCTTCA
    GAAAGTCCTG CTAGTCTGAA AAGAGTAGCA TCACCAGCAG CTAAAGCAAC TATGTCAGGT
    GAAGAATATG TTGCTATGTA TACTTATGAG AGTTCTGAAC AAGGAGACCT AACCTTTCAG
    CAGGGTGATA TGATTCTTGT GACAAAGAAA GATGGCGACT GGTGGACAGG AACACTGGGT
    GATAAAACAG GAGTTTTCCC ATCAAATTAT GTGAGACTCA AAGATTCTGA GGCTTCTGGA
    GCAGCTGGAA AAACAGGAAG CTTAGGGAAG AAACCTGAAA TTGCCCAAGT TATTGCCTCT
    TACACAGCAA CGGGTCCAGA ACAACTCACC CTTGCTCCTG GTCAGTTGAT TCTTATCAGA
    AAGAAGAACC CAGGTGGCTG GTGGGAAGGA GAGCTCCAAG CACGAGGGAA AAAACGACAA
    ATAGGATGGT TTCCTGCTAA TTATGTGAAA CTTTTGAGCC CTGGTACCAG TAAAACTACA
    CCAACCGAGC TACCTAAATC AACAGCATTA CCTTCAGTGT GCCAAGTAAT TGGCATGTAT
    GACTACACTG CACAGAATGA TGATGAGCTA GCATTCAATA AAGGCCAGAT TATAAACGTC
    CTTAACAAGG AAGACCCAGA CTGGTGGAAA GGGGAGGTGA ATGGACAAGT GGGTCTCTTC
    CCATCCAACT ATGTGAAGCT GACAACAGAT ATGGACCCAA GCCAGCAATG GTGTGCAGAC
    TTGCATCTTC TGGATATGCT GACACCTACT GAACGAAAAA GGCAGGGATA CATCCATGAG
    CTCATTGTCA CAGAAGAGAA CTACGTAAAT GATCTGCAGT TGGTCACAGA GATCTTCCAG
    AAACCACTAA TGGAATCTGA GCTGCTAACA GAAAAGGAAG TTGCTATGAT TTTTGTTAAT
    TGGAAGGAGC TGATTATGTG CAATATAAAA CTACTGAAGG CCTTGCGTGT GCGTAAGAAG
    ATGTCTGGGG AGAAGATGCC AGTGAAAATG ATTGGTGACA TCCTGACAGC TCAGCTGCCA
    CACATGCAGC CATACATTCG GTTCTGTAGC TGCCAGCTCA ATGGGGCAGC ACTCATTCAG
    CAGAAAACAG ATGAAGTCCC CGAATTCAAG GAGTTTGTAA AAAGGTTAGC AATGGATCCT
    CGCTGTAAAG GAATGCCACT ATCAAGTTTT CTACTAAAGC CTATGCAACG GGTAACAAGA
    TACCCACTAA TAATTAAAAA TATAATAGAA AACACTCCTG AAAACCATCC TGACCACAGT
    CACTTGAAGC ATGCTCTTGA AAAAGCAGAA GAATTATGTT CTCAAGTAAA TGAAGGAGTG
    CGAGAGAAAG AAAATTCAGA TAGGCTGGAG TGGATCCAGG CTCATGTTCA GTGCGAAGGC
    CTCTCTGAGC AACTCGTATT TAATTCTGTT ACAAACTGCT TGGGACCACG CAAGTTTCTG
    CACAGTGGGA AGCTCTATAA AGCCAAGAGT AACAAGGAAC TGTATGGCTT TCTGTTCAAC
    GATTTCCTCC TCTTGACTCA GATCATAAAA CCTCTTGGCT CTTCTGGCAC AGACAAGGTC
    TTCAGCCCCA AGTCTAATCT GCAGTACAAA ATGTACAAAA CTCCCATTTT TCTAAATGAA
    GTACTAGTAA AATTACCCAC AGACCCTTCT GGAGATGAGC CCATTTTCCA TATCTCCCAC
    ATTGACAGAG TCTACACACT CCGGGCTGAA AGCATTAATG AAAGGACTGC GTGGGTTCAG
    AAAATTAAAG CTGCTTCGGA ACTTTACATA GAGACTGAAA AGAAGAAGAG GGAAAAGGCC
    TACTTAGTTC GCTCACAAAG AGCAACAGGC ATCGGGAGGT TGATGGTGAA TATTGTTGAA
    GGCATTGAAC TAAAACCTTG CAGATCCCAT GGAAAGAGTA ATCCATACTG TGAGGTGACG
    ATGGGCTCTC AGTGCCACAT CACCAAGACA ATGCAGGACA CCCTCAATCC AAAGTGGAAC
    TCCAACTGCC AGTTCTTTAT CAAAGACCTC GAGCAGGACG TGCTCTGCAT CACGGTGTTT
    GAGAGGGACC AGTTCTCCCC AGATGACTTT TTGGGCAGAA CAGAGATCCG TGTGGCAGAC
    ATTAAGAAGG ATCAGGGTTC AAAGGGACCA GTTACTAAGT GTCTCCTTCT CCATGAAGTC
    CCAACAGGAG AGATAGTCGT CCGACTAGAC CTGCAATTGT TTGATGAGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015155508.1
    CDS351..5384
    Translation

    Target ORF information:

    RefSeq Version XM_015300022.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015300022.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAACAATG ATGGAAGGAT GGATCAGCTG GAGTTTTCAA TAGCTATGAA ACTTATCAAA 
    TTAAAACTAC AAGGCTATCA ACTCCCATCT GCGCTGCCTC CTGTCATGAA GCAGCCACCT
    ATTGCTCTAC CTAGTGCACC AGGGTTTGGT ATTGGGGGCA TTGCCAGCAT GCCATCTCTT
    ACAACTGTTG CCCCAGTGCC AATGGCATCT ATTCCAGTAG TAGGAATGTC TCCGCCATTA
    GTATCTTCTG TTCCTGCAGC AGCAGTGCCT CCCCTGGCTA ATGGAGCTCC TGCTGTTATA
    CAGCCTCTGC CTGCTTTTGC TCATCCTGCC ACATTGCCAA AGAGTTCTTC CTTTAGTAGA
    TCTGGTCCAG GATCGCAGCT GAACACAAAA CTGCAAAAGG CACAATCGTT TGACGTGGCG
    AGTGTGCCTC CTGTGGCAGA GTGGGCAGTA CCTCAGTCAT CAAGACTGAA ATACAGACAG
    TTGTTCAATA GCCACGATAA AACAATGAGT GGACACTTAA CAGGTCCGCA AGCAAGAACT
    ATTCTTATGC AGTCAAGTTT ACCCCAGGCT CAGCTGGCTA CAATATGGAA TCTTTCAGAT
    ATTGATCAAG ATGGAAAGCT TACAGCTGAA GAGTTTATTC TGGCTATGCA CTTAATTGAT
    GTAGCTATGT CTGGTCAGCC ACTGCCACCT GTACTGCCTC CAGAGTATAT TCCACCTTCA
    TTTAGAAGAG TACGCTCTGG TAGTGGTGTA TCTGCTGTAA GTTCAGTATC TGTAGACCAA
    AGGTTACCAG AAGAACCAGC ATTAGAAGAA GAACAGCAGC AACTGGAAAA GAAATTGCCA
    GTTACATTTG AAGATAAAAA ACGTGAGAAC TTTGAACGTG GCAATCTAGA ACTTGAAAAA
    CGGAGGCAGG CTCTCCTGGA ACAGCAACGC AAAGAGCAAG AGCGTCTAGC ACAGCTGGAA
    CGGGCAGAGC AAGAAAGGAA GGAACGTGAA CGACAAGAGC AAGAAAGGAA AAGACAGCTG
    GAGCTAGAGA AACAGTTGGA GAAACAACGG GAATTGGAAC GGCAGAGAGA AGAGGAAAGG
    AGGAAAGAAA TAGAAAGAAG AGAGGCTGCA AAACGGGAAC TTGAGAGGCA AAGGCAACTT
    GAATGGGAAC GTAATCGTCG GCAAGAACTG CTAAATCAAA GAAACAGAGA ACAAGAGGAC
    ATCGTTGTTC TGAAGGCAAA GAAGAAGACT TTAGAATTTG AGCTGGAAGC TCTAAATGAT
    AAAAAAAATC AGTTGGAAGG AAAGCTTCAG GATATCAGAT GTCGGCTGTC TACCCAAAGA
    CAAGAAATTG AAAGTACAAA TAAATCTAGA GAACTGAGAA TTGCAGAAAT CACCCATTTG
    CAACAGCAGC TACAGGAGTC TCAGCAGATG CTTGGAAAGT TGATTCCAGA AAAGCAATTA
    CTCAATGACC AACTAAAGCA GGTTCAACAG AACAGTTTGC ATAGAGATTC TCTTCTTACT
    ATCAAAAGAG CCTTAGAAGC CAAGGAACTA GCCCGTCAAC AGCTTCGAGA CCAGCTAGAT
    GAAGTAGAAA AAGAAACCAG ATCTAAACTT CAGGAAATTG ATATTTTCAA TAATCAGCTG
    AAGTCAATGG CAATGGAAGA TGCAATGCTG CAGTGCCTGG CAGTTCTCCT GGGCTGCGTC
    AACTACTTAG ACTCCTTTCT AAAGGAGCTG AGAGAAATAC ATAACAGACA GCAGCTTCAG
    AAACAAAAGA ACTTGGAAGC TGAGAGGCTG AAACAGAAAG AACAAGAAAG GAAGACAGAA
    CTGGAAAAGC AAAAAGAAGC TCAAAGGCGA ATCCAGGATC GGGATAAACA ACGGCTTGAT
    CGAGTGCAAC AAGAAGAAGA ACCTCAATGG CAAAAAAAAA ATCAAGAAGA TGACAAGCAA
    AAAAGGGAAG AAATAATCAA GAAGAAGGAA AGTGAGGATA AGGGGAAACA GGAAATCCAA
    GAGAAACCGA GTAAGCTTTT CCAACCACAT CAAGAACCAG TTAAGCCAGC TGTCCAGGCT
    CCATGGTCAA ATGCAGGAAA AGCTCCACTA ACAATTTCTG CTCAGGAGGA TGTAAAAATA
    GTGTATTATA GAGCACTGTA TCCTTTTGAA TCAAGAAGCC ATGACGAAAT CACTATTCAA
    CCTGGCGACA TAGTCATGGT TAAAAGGGAA TGGGTGGATG AAAGTCAGAC TGGAGAACCA
    GGGTGGCTTG GTGGAGAATT GAAGGGAAAA ACTGGATGGT TCCCAGCAAA CTACGCAGAA
    AAAATACCTG AAAGTGAAGT TCCAGCATCT ATAAAACCAG TTGAGGCAGC TCCTGCACCT
    AAAGTTTCTG TGCATGAAAC AACCACATCA CTAGGAACTG CTGCATCTAC AGAGTGCACG
    ACAACTGCTA ACAACTGGGC AGACTTCAGT TCAACATGGC CAGCAAACAC TAATGAGAAA
    CCAGAGACAG ACAACTGGGA TGCCTGGGCA GCTCAACCAT CTTTGACTGT TCCCAGTGCA
    GGGCAGCTGC GGCAGCGATC AGCCTTCACC CCCGCCACTG TTACAGGATC GTCTCCCTCT
    CCTGTATTGG GTCAGGGTGA AAAAGTGGAG GGACTTCAAG CACAGGCTCT GTATCCTTGG
    AGAGCAAAAA AAGACAACCA CCTAAATTTC AACAAAAATG ATGTCATCAC AGTTTTAGAG
    CAGCAAGATA TGTGGTGGTT TGGAGAAGTT CAAGGACAAA AGGGGTGGTT TCCTAAGTCA
    TATGTGAAGC TTATTTCAGG CCCCATTAGG AAGTCAACAA GCATGGATTC TGGTTCTTCA
    GAAAGTCCTG CTAGTCTGAA AAGAGTAGCA TCACCAGCAG CTAAAGCAAC TATGTCAGGT
    GAAGAATATG TTGCTATGTA TACTTATGAG AGTTCTGAAC AAGGAGACCT AACCTTTCAG
    CAGGGTGATA TGATTCTTGT GACAAAGAAA GATGGCGACT GGTGGACAGG AACACTGGGT
    GATAAAACAG GAGTTTTCCC ATCAAATTAT GTGAGACTCA AAGATTCTGA GGCTTCTGGA
    GCAGCTGGAA AAACAGGAAG CTTAGGGAAG AAACCTGAAA TTGCCCAAGT TATTGCCTCT
    TACACAGCAA CGGGTCCAGA ACAACTCACC CTTGCTCCTG GTCAGTTGAT TCTTATCAGA
    AAGAAGAACC CAGGTGGCTG GTGGGAAGGA GAGCTCCAAG CACGAGGGAA AAAACGACAA
    ATAGGATGGT TTCCTGCTAA TTATGTGAAA CTTTTGAGCC CTGGTACCAG TAAAACTACA
    CCAACCGAGC TACCTAAATC AACAGCATTA CCTTCAGTGT GCCAAGTAAT TGGCATGTAT
    GACTACACTG CACAGAATGA TGATGAGCTA GCATTCAATA AAGGCCAGAT TATAAACGTC
    CTTAACAAGG AAGACCCAGA CTGGTGGAAA GGGGAGGTGA ATGGACAAGT GGGTCTCTTC
    CCATCCAACT ATGTGAAGCT GACAACAGAT ATGGACCCAA GCCAGCAATG GTGTGCAGAC
    TTGCATCTTC TGGATATGCT GACACCTACT GAACGAAAAA GGCAGGGATA CATCCATGAG
    CTCATTGTCA CAGAAGAGAA CTACGTAAAT GATCTGCAGT TGGTCACAGA GATCTTCCAG
    AAACCACTAA TGGAATCTGA GCTGCTAACA GAAAAGGAAG TTGCTATGAT TTTTGTTAAT
    TGGAAGGAGC TGATTATGTG CAATATAAAA CTACTGAAGG CCTTGCGTGT GCGTAAGAAG
    ATGTCTGGGG AGAAGATGCC AGTGAAAATG ATTGGTGACA TCCTGACAGC TCAGCTGCCA
    CACATGCAGC CATACATTCG GTTCTGTAGC TGCCAGCTCA ATGGGGCAGC ACTCATTCAG
    CAGAAAACAG ATGAAGTCCC CGAATTCAAG GAGTTTGTAA AAAGGTTAGC AATGGATCCT
    CGCTGTAAAG GAATGCCACT ATCAAGTTTT CTACTAAAGC CTATGCAACG GGTAACAAGA
    TACCCACTAA TAATTAAAAA TATAATAGAA AACACTCCTG AAAACCATCC TGACCACAGT
    CACTTGAAGC ATGCTCTTGA AAAAGCAGAA GAATTATGTT CTCAAGTAAA TGAAGGAGTG
    CGAGAGAAAG AAAATTCAGA TAGGCTGGAG TGGATCCAGG CTCATGTTCA GTGCGAAGGC
    CTCTCTGAGC AACTCGTATT TAATTCTGTT ACAAACTGCT TGGGACCACG CAAGTTTCTG
    CACAGTGGGA AGCTCTATAA AGCCAAGAGT AACAAGGAAC TGTATGGCTT TCTGTTCAAC
    GATTTCCTCC TCTTGACTCA GATCATAAAA CCTCTTGGCT CTTCTGGCAC AGACAAGGTC
    TTCAGCCCCA AGTCTAATCT GCAGTACAAA ATGTACAAAA CTCCCATTTT TCTAAATGAA
    GTACTAGTAA AATTACCCAC AGACCCTTCT GGAGATGAGC CCATTTTCCA TATCTCCCAC
    ATTGACAGAG TCTACACACT CCGGGCTGAA AGCATTAATG AAAGGACTGC GTGGGTTCAG
    AAAATTAAAG CTGCTTCGGA ACTTTACATA GAGACTGAAA AGAAGAAGAG GGAAAAGGCC
    TACTTAGTTC GCTCACAAAG AGCAACAGGC ATCGGGAGGT TGATGGTGAA TATTGTTGAA
    GGCATTGAAC TAAAACCTTG CAGATCCCAT GGAAAGAGTA ATCCATACTG TGAGGTGACG
    ATGGGCTCTC AGTGCCACAT CACCAAGACA ATGCAGGACA CCCTCAATCC AAAGTGGAAC
    TCCAACTGCC AGTTCTTTAT CAAAGACCTC GAGCAGGACG TGCTCTGCAT CACGGTGTTT
    GAGAGGGACC AGTTCTCCCC AGATGACTTT TTGGGCAGAA CAGAGATCCG TGTGGCAGAC
    ATTAAGAAGG ATCAGGGTTC AAAGGGACCA GTTACTAAGT GTCTCCTTCT CCATGAAGTC
    CCAACAGGAG AGATAGTCGT CCGACTAGAC CTGCAATTGT TTGATGAGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30288
    Clone ID Related Accession (Same CDS sequence) XM_015300022.1 , XM_015300022.2
    Accession Version XM_015300022.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product intersectin-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015300022.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAACAATG ATGGAAGGAT GGATCAGCTG GAGTTTTCAA TAGCTATGAA ACTTATCAAA 
    TTAAAACTAC AAGGCTATCA ACTCCCATCT GCGCTGCCTC CTGTCATGAA GCAGCCACCT
    ATTGCTCTAC CTAGTGCACC AGGGTTTGGT ATTGGGGGCA TTGCCAGCAT GCCATCTCTT
    ACAACTGTTG CCCCAGTGCC AATGGCATCT ATTCCAGTAG TAGGAATGTC TCCGCCATTA
    GTATCTTCTG TTCCTGCAGC AGCAGTGCCT CCCCTGGCTA ATGGAGCTCC TGCTGTTATA
    CAGCCTCTGC CTGCTTTTGC TCATCCTGCC ACATTGCCAA AGAGTTCTTC CTTTAGTAGA
    TCTGGTCCAG GATCGCAGCT GAACACAAAA CTGCAAAAGG CACAATCGTT TGACGTGGCG
    AGTGTGCCTC CTGTGGCAGA GTGGGCAGTA CCTCAGTCAT CAAGACTGAA ATACAGACAG
    TTGTTCAATA GCCACGATAA AACAATGAGT GGACACTTAA CAGGTCCGCA AGCAAGAACT
    ATTCTTATGC AGTCAAGTTT ACCCCAGGCT CAGCTGGCTA CAATATGGAA TCTTTCAGAT
    ATTGATCAAG ATGGAAAGCT TACAGCTGAA GAGTTTATTC TGGCTATGCA CTTAATTGAT
    GTAGCTATGT CTGGTCAGCC ACTGCCACCT GTACTGCCTC CAGAGTATAT TCCACCTTCA
    TTTAGAAGAG TACGCTCTGG TAGTGGTGTA TCTGCTGTAA GTTCAGTATC TGTAGACCAA
    AGGTTACCAG AAGAACCAGC ATTAGAAGAA GAACAGCAGC AACTGGAAAA GAAATTGCCA
    GTTACATTTG AAGATAAAAA ACGTGAGAAC TTTGAACGTG GCAATCTAGA ACTTGAAAAA
    CGGAGGCAGG CTCTCCTGGA ACAGCAACGC AAAGAGCAAG AGCGTCTAGC ACAGCTGGAA
    CGGGCAGAGC AAGAAAGGAA GGAACGTGAA CGACAAGAGC AAGAAAGGAA AAGACAGCTG
    GAGCTAGAGA AACAGTTGGA GAAACAACGG GAATTGGAAC GGCAGAGAGA AGAGGAAAGG
    AGGAAAGAAA TAGAAAGAAG AGAGGCTGCA AAACGGGAAC TTGAGAGGCA AAGGCAACTT
    GAATGGGAAC GTAATCGTCG GCAAGAACTG CTAAATCAAA GAAACAGAGA ACAAGAGGAC
    ATCGTTGTTC TGAAGGCAAA GAAGAAGACT TTAGAATTTG AGCTGGAAGC TCTAAATGAT
    AAAAAAAATC AGTTGGAAGG AAAGCTTCAG GATATCAGAT GTCGGCTGTC TACCCAAAGA
    CAAGAAATTG AAAGTACAAA TAAATCTAGA GAACTGAGAA TTGCAGAAAT CACCCATTTG
    CAACAGCAGC TACAGGAGTC TCAGCAGATG CTTGGAAAGT TGATTCCAGA AAAGCAATTA
    CTCAATGACC AACTAAAGCA GGTTCAACAG AACAGTTTGC ATAGAGATTC TCTTCTTACT
    ATCAAAAGAG CCTTAGAAGC CAAGGAACTA GCCCGTCAAC AGCTTCGAGA CCAGCTAGAT
    GAAGTAGAAA AAGAAACCAG ATCTAAACTT CAGGAAATTG ATATTTTCAA TAATCAGCTG
    AAGTCAATGG CAATGGAAGA TGCAATGCTG CAGTGCCTGG CAGTTCTCCT GGGCTGCGTC
    AACTACTTAG ACTCCTTTCT AAAGGAGCTG AGAGAAATAC ATAACAGACA GCAGCTTCAG
    AAACAAAAGA ACTTGGAAGC TGAGAGGCTG AAACAGAAAG AACAAGAAAG GAAGACAGAA
    CTGGAAAAGC AAAAAGAAGC TCAAAGGCGA ATCCAGGATC GGGATAAACA ACGGCTTGAT
    CGAGTGCAAC AAGAAGAAGA ACCTCAATGG CAAAAAAAAA ATCAAGAAGA TGACAAGCAA
    AAAAGGGAAG AAATAATCAA GAAGAAGGAA AGTGAGGATA AGGGGAAACA GGAAATCCAA
    GAGAAACCGA GTAAGCTTTT CCAACCACAT CAAGAACCAG TTAAGCCAGC TGTCCAGGCT
    CCATGGTCAA ATGCAGGAAA AGCTCCACTA ACAATTTCTG CTCAGGAGGA TGTAAAAATA
    GTGTATTATA GAGCACTGTA TCCTTTTGAA TCAAGAAGCC ATGACGAAAT CACTATTCAA
    CCTGGCGACA TAGTCATGGT TAAAAGGGAA TGGGTGGATG AAAGTCAGAC TGGAGAACCA
    GGGTGGCTTG GTGGAGAATT GAAGGGAAAA ACTGGATGGT TCCCAGCAAA CTACGCAGAA
    AAAATACCTG AAAGTGAAGT TCCAGCATCT ATAAAACCAG TTGAGGCAGC TCCTGCACCT
    AAAGTTTCTG TGCATGAAAC AACCACATCA CTAGGAACTG CTGCATCTAC AGAGTGCACG
    ACAACTGCTA ACAACTGGGC AGACTTCAGT TCAACATGGC CAGCAAACAC TAATGAGAAA
    CCAGAGACAG ACAACTGGGA TGCCTGGGCA GCTCAACCAT CTTTGACTGT TCCCAGTGCA
    GGGCAGCTGC GGCAGCGATC AGCCTTCACC CCCGCCACTG TTACAGGATC GTCTCCCTCT
    CCTGTATTGG GTCAGGGTGA AAAAGTGGAG GGACTTCAAG CACAGGCTCT GTATCCTTGG
    AGAGCAAAAA AAGACAACCA CCTAAATTTC AACAAAAATG ATGTCATCAC AGTTTTAGAG
    CAGCAAGATA TGTGGTGGTT TGGAGAAGTT CAAGGACAAA AGGGGTGGTT TCCTAAGTCA
    TATGTGAAGC TTATTTCAGG CCCCATTAGG AAGTCAACAA GCATGGATTC TGGTTCTTCA
    GAAAGTCCTG CTAGTCTGAA AAGAGTAGCA TCACCAGCAG CTAAAGCAAC TATGTCAGGT
    GAAGAATATG TTGCTATGTA TACTTATGAG AGTTCTGAAC AAGGAGACCT AACCTTTCAG
    CAGGGTGATA TGATTCTTGT GACAAAGAAA GATGGCGACT GGTGGACAGG AACACTGGGT
    GATAAAACAG GAGTTTTCCC ATCAAATTAT GTGAGACTCA AAGATTCTGA GGCTTCTGGA
    GCAGCTGGAA AAACAGGAAG CTTAGGGAAG AAACCTGAAA TTGCCCAAGT TATTGCCTCT
    TACACAGCAA CGGGTCCAGA ACAACTCACC CTTGCTCCTG GTCAGTTGAT TCTTATCAGA
    AAGAAGAACC CAGGTGGCTG GTGGGAAGGA GAGCTCCAAG CACGAGGGAA AAAACGACAA
    ATAGGATGGT TTCCTGCTAA TTATGTGAAA CTTTTGAGCC CTGGTACCAG TAAAACTACA
    CCAACCGAGC TACCTAAATC AACAGCATTA CCTTCAGTGT GCCAAGTAAT TGGCATGTAT
    GACTACACTG CACAGAATGA TGATGAGCTA GCATTCAATA AAGGCCAGAT TATAAACGTC
    CTTAACAAGG AAGACCCAGA CTGGTGGAAA GGGGAGGTGA ATGGACAAGT GGGTCTCTTC
    CCATCCAACT ATGTGAAGCT GACAACAGAT ATGGACCCAA GCCAGCAATG GTGTGCAGAC
    TTGCATCTTC TGGATATGCT GACACCTACT GAACGAAAAA GGCAGGGATA CATCCATGAG
    CTCATTGTCA CAGAAGAGAA CTACGTAAAT GATCTGCAGT TGGTCACAGA GATCTTCCAG
    AAACCACTAA TGGAATCTGA GCTGCTAACA GAAAAGGAAG TTGCTATGAT TTTTGTTAAT
    TGGAAGGAGC TGATTATGTG CAATATAAAA CTACTGAAGG CCTTGCGTGT GCGTAAGAAG
    ATGTCTGGGG AGAAGATGCC AGTGAAAATG ATTGGTGACA TCCTGACAGC TCAGCTGCCA
    CACATGCAGC CATACATTCG GTTCTGTAGC TGCCAGCTCA ATGGGGCAGC ACTCATTCAG
    CAGAAAACAG ATGAAGTCCC CGAATTCAAG GAGTTTGTAA AAAGGTTAGC AATGGATCCT
    CGCTGTAAAG GAATGCCACT ATCAAGTTTT CTACTAAAGC CTATGCAACG GGTAACAAGA
    TACCCACTAA TAATTAAAAA TATAATAGAA AACACTCCTG AAAACCATCC TGACCACAGT
    CACTTGAAGC ATGCTCTTGA AAAAGCAGAA GAATTATGTT CTCAAGTAAA TGAAGGAGTG
    CGAGAGAAAG AAAATTCAGA TAGGCTGGAG TGGATCCAGG CTCATGTTCA GTGCGAAGGC
    CTCTCTGAGC AACTCGTATT TAATTCTGTT ACAAACTGCT TGGGACCACG CAAGTTTCTG
    CACAGTGGGA AGCTCTATAA AGCCAAGAGT AACAAGGAAC TGTATGGCTT TCTGTTCAAC
    GATTTCCTCC TCTTGACTCA GATCATAAAA CCTCTTGGCT CTTCTGGCAC AGACAAGGTC
    TTCAGCCCCA AGTCTAATCT GCAGTACAAA ATGTACAAAA CTCCCATTTT TCTAAATGAA
    GTACTAGTAA AATTACCCAC AGACCCTTCT GGAGATGAGC CCATTTTCCA TATCTCCCAC
    ATTGACAGAG TCTACACACT CCGGGCTGAA AGCATTAATG AAAGGACTGC GTGGGTTCAG
    AAAATTAAAG CTGCTTCGGA ACTTTACATA GAGACTGAAA AGAAGAAGAG GGAAAAGGCC
    TACTTAGTTC GCTCACAAAG AGCAACAGGC ATCGGGAGGT TGATGGTGAA TATTGTTGAA
    GGCATTGAAC TAAAACCTTG CAGATCCCAT GGAAAGAGTA ATCCATACTG TGAGGTGACG
    ATGGGCTCTC AGTGCCACAT CACCAAGACA ATGCAGGACA CCCTCAATCC AAAGTGGAAC
    TCCAACTGCC AGTTCTTTAT CAAAGACCTC GAGCAGGACG TGCTCTGCAT CACGGTGTTT
    GAGAGGGACC AGTTCTCCCC AGATGACTTT TTGGGCAGAA CAGAGATCCG TGTGGCAGAC
    ATTAAGAAGG ATCAGGGTTC AAAGGGACCA GTTACTAAGT GTCTCCTTCT CCATGAAGTC
    CCAACAGGAG AGATAGTCGT CCGACTAGAC CTGCAATTGT TTGATGAGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015155508.1
    CDS369..5402
    Translation

    Target ORF information:

    RefSeq Version XM_015300022.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus intersectin 1 (ITSN1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015300022.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAACAATG ATGGAAGGAT GGATCAGCTG GAGTTTTCAA TAGCTATGAA ACTTATCAAA 
    TTAAAACTAC AAGGCTATCA ACTCCCATCT GCGCTGCCTC CTGTCATGAA GCAGCCACCT
    ATTGCTCTAC CTAGTGCACC AGGGTTTGGT ATTGGGGGCA TTGCCAGCAT GCCATCTCTT
    ACAACTGTTG CCCCAGTGCC AATGGCATCT ATTCCAGTAG TAGGAATGTC TCCGCCATTA
    GTATCTTCTG TTCCTGCAGC AGCAGTGCCT CCCCTGGCTA ATGGAGCTCC TGCTGTTATA
    CAGCCTCTGC CTGCTTTTGC TCATCCTGCC ACATTGCCAA AGAGTTCTTC CTTTAGTAGA
    TCTGGTCCAG GATCGCAGCT GAACACAAAA CTGCAAAAGG CACAATCGTT TGACGTGGCG
    AGTGTGCCTC CTGTGGCAGA GTGGGCAGTA CCTCAGTCAT CAAGACTGAA ATACAGACAG
    TTGTTCAATA GCCACGATAA AACAATGAGT GGACACTTAA CAGGTCCGCA AGCAAGAACT
    ATTCTTATGC AGTCAAGTTT ACCCCAGGCT CAGCTGGCTA CAATATGGAA TCTTTCAGAT
    ATTGATCAAG ATGGAAAGCT TACAGCTGAA GAGTTTATTC TGGCTATGCA CTTAATTGAT
    GTAGCTATGT CTGGTCAGCC ACTGCCACCT GTACTGCCTC CAGAGTATAT TCCACCTTCA
    TTTAGAAGAG TACGCTCTGG TAGTGGTGTA TCTGCTGTAA GTTCAGTATC TGTAGACCAA
    AGGTTACCAG AAGAACCAGC ATTAGAAGAA GAACAGCAGC AACTGGAAAA GAAATTGCCA
    GTTACATTTG AAGATAAAAA ACGTGAGAAC TTTGAACGTG GCAATCTAGA ACTTGAAAAA
    CGGAGGCAGG CTCTCCTGGA ACAGCAACGC AAAGAGCAAG AGCGTCTAGC ACAGCTGGAA
    CGGGCAGAGC AAGAAAGGAA GGAACGTGAA CGACAAGAGC AAGAAAGGAA AAGACAGCTG
    GAGCTAGAGA AACAGTTGGA GAAACAACGG GAATTGGAAC GGCAGAGAGA AGAGGAAAGG
    AGGAAAGAAA TAGAAAGAAG AGAGGCTGCA AAACGGGAAC TTGAGAGGCA AAGGCAACTT
    GAATGGGAAC GTAATCGTCG GCAAGAACTG CTAAATCAAA GAAACAGAGA ACAAGAGGAC
    ATCGTTGTTC TGAAGGCAAA GAAGAAGACT TTAGAATTTG AGCTGGAAGC TCTAAATGAT
    AAAAAAAATC AGTTGGAAGG AAAGCTTCAG GATATCAGAT GTCGGCTGTC TACCCAAAGA
    CAAGAAATTG AAAGTACAAA TAAATCTAGA GAACTGAGAA TTGCAGAAAT CACCCATTTG
    CAACAGCAGC TACAGGAGTC TCAGCAGATG CTTGGAAAGT TGATTCCAGA AAAGCAATTA
    CTCAATGACC AACTAAAGCA GGTTCAACAG AACAGTTTGC ATAGAGATTC TCTTCTTACT
    ATCAAAAGAG CCTTAGAAGC CAAGGAACTA GCCCGTCAAC AGCTTCGAGA CCAGCTAGAT
    GAAGTAGAAA AAGAAACCAG ATCTAAACTT CAGGAAATTG ATATTTTCAA TAATCAGCTG
    AAGTCAATGG CAATGGAAGA TGCAATGCTG CAGTGCCTGG CAGTTCTCCT GGGCTGCGTC
    AACTACTTAG ACTCCTTTCT AAAGGAGCTG AGAGAAATAC ATAACAGACA GCAGCTTCAG
    AAACAAAAGA ACTTGGAAGC TGAGAGGCTG AAACAGAAAG AACAAGAAAG GAAGACAGAA
    CTGGAAAAGC AAAAAGAAGC TCAAAGGCGA ATCCAGGATC GGGATAAACA ACGGCTTGAT
    CGAGTGCAAC AAGAAGAAGA ACCTCAATGG CAAAAAAAAA ATCAAGAAGA TGACAAGCAA
    AAAAGGGAAG AAATAATCAA GAAGAAGGAA AGTGAGGATA AGGGGAAACA GGAAATCCAA
    GAGAAACCGA GTAAGCTTTT CCAACCACAT CAAGAACCAG TTAAGCCAGC TGTCCAGGCT
    CCATGGTCAA ATGCAGGAAA AGCTCCACTA ACAATTTCTG CTCAGGAGGA TGTAAAAATA
    GTGTATTATA GAGCACTGTA TCCTTTTGAA TCAAGAAGCC ATGACGAAAT CACTATTCAA
    CCTGGCGACA TAGTCATGGT TAAAAGGGAA TGGGTGGATG AAAGTCAGAC TGGAGAACCA
    GGGTGGCTTG GTGGAGAATT GAAGGGAAAA ACTGGATGGT TCCCAGCAAA CTACGCAGAA
    AAAATACCTG AAAGTGAAGT TCCAGCATCT ATAAAACCAG TTGAGGCAGC TCCTGCACCT
    AAAGTTTCTG TGCATGAAAC AACCACATCA CTAGGAACTG CTGCATCTAC AGAGTGCACG
    ACAACTGCTA ACAACTGGGC AGACTTCAGT TCAACATGGC CAGCAAACAC TAATGAGAAA
    CCAGAGACAG ACAACTGGGA TGCCTGGGCA GCTCAACCAT CTTTGACTGT TCCCAGTGCA
    GGGCAGCTGC GGCAGCGATC AGCCTTCACC CCCGCCACTG TTACAGGATC GTCTCCCTCT
    CCTGTATTGG GTCAGGGTGA AAAAGTGGAG GGACTTCAAG CACAGGCTCT GTATCCTTGG
    AGAGCAAAAA AAGACAACCA CCTAAATTTC AACAAAAATG ATGTCATCAC AGTTTTAGAG
    CAGCAAGATA TGTGGTGGTT TGGAGAAGTT CAAGGACAAA AGGGGTGGTT TCCTAAGTCA
    TATGTGAAGC TTATTTCAGG CCCCATTAGG AAGTCAACAA GCATGGATTC TGGTTCTTCA
    GAAAGTCCTG CTAGTCTGAA AAGAGTAGCA TCACCAGCAG CTAAAGCAAC TATGTCAGGT
    GAAGAATATG TTGCTATGTA TACTTATGAG AGTTCTGAAC AAGGAGACCT AACCTTTCAG
    CAGGGTGATA TGATTCTTGT GACAAAGAAA GATGGCGACT GGTGGACAGG AACACTGGGT
    GATAAAACAG GAGTTTTCCC ATCAAATTAT GTGAGACTCA AAGATTCTGA GGCTTCTGGA
    GCAGCTGGAA AAACAGGAAG CTTAGGGAAG AAACCTGAAA TTGCCCAAGT TATTGCCTCT
    TACACAGCAA CGGGTCCAGA ACAACTCACC CTTGCTCCTG GTCAGTTGAT TCTTATCAGA
    AAGAAGAACC CAGGTGGCTG GTGGGAAGGA GAGCTCCAAG CACGAGGGAA AAAACGACAA
    ATAGGATGGT TTCCTGCTAA TTATGTGAAA CTTTTGAGCC CTGGTACCAG TAAAACTACA
    CCAACCGAGC TACCTAAATC AACAGCATTA CCTTCAGTGT GCCAAGTAAT TGGCATGTAT
    GACTACACTG CACAGAATGA TGATGAGCTA GCATTCAATA AAGGCCAGAT TATAAACGTC
    CTTAACAAGG AAGACCCAGA CTGGTGGAAA GGGGAGGTGA ATGGACAAGT GGGTCTCTTC
    CCATCCAACT ATGTGAAGCT GACAACAGAT ATGGACCCAA GCCAGCAATG GTGTGCAGAC
    TTGCATCTTC TGGATATGCT GACACCTACT GAACGAAAAA GGCAGGGATA CATCCATGAG
    CTCATTGTCA CAGAAGAGAA CTACGTAAAT GATCTGCAGT TGGTCACAGA GATCTTCCAG
    AAACCACTAA TGGAATCTGA GCTGCTAACA GAAAAGGAAG TTGCTATGAT TTTTGTTAAT
    TGGAAGGAGC TGATTATGTG CAATATAAAA CTACTGAAGG CCTTGCGTGT GCGTAAGAAG
    ATGTCTGGGG AGAAGATGCC AGTGAAAATG ATTGGTGACA TCCTGACAGC TCAGCTGCCA
    CACATGCAGC CATACATTCG GTTCTGTAGC TGCCAGCTCA ATGGGGCAGC ACTCATTCAG
    CAGAAAACAG ATGAAGTCCC CGAATTCAAG GAGTTTGTAA AAAGGTTAGC AATGGATCCT
    CGCTGTAAAG GAATGCCACT ATCAAGTTTT CTACTAAAGC CTATGCAACG GGTAACAAGA
    TACCCACTAA TAATTAAAAA TATAATAGAA AACACTCCTG AAAACCATCC TGACCACAGT
    CACTTGAAGC ATGCTCTTGA AAAAGCAGAA GAATTATGTT CTCAAGTAAA TGAAGGAGTG
    CGAGAGAAAG AAAATTCAGA TAGGCTGGAG TGGATCCAGG CTCATGTTCA GTGCGAAGGC
    CTCTCTGAGC AACTCGTATT TAATTCTGTT ACAAACTGCT TGGGACCACG CAAGTTTCTG
    CACAGTGGGA AGCTCTATAA AGCCAAGAGT AACAAGGAAC TGTATGGCTT TCTGTTCAAC
    GATTTCCTCC TCTTGACTCA GATCATAAAA CCTCTTGGCT CTTCTGGCAC AGACAAGGTC
    TTCAGCCCCA AGTCTAATCT GCAGTACAAA ATGTACAAAA CTCCCATTTT TCTAAATGAA
    GTACTAGTAA AATTACCCAC AGACCCTTCT GGAGATGAGC CCATTTTCCA TATCTCCCAC
    ATTGACAGAG TCTACACACT CCGGGCTGAA AGCATTAATG AAAGGACTGC GTGGGTTCAG
    AAAATTAAAG CTGCTTCGGA ACTTTACATA GAGACTGAAA AGAAGAAGAG GGAAAAGGCC
    TACTTAGTTC GCTCACAAAG AGCAACAGGC ATCGGGAGGT TGATGGTGAA TATTGTTGAA
    GGCATTGAAC TAAAACCTTG CAGATCCCAT GGAAAGAGTA ATCCATACTG TGAGGTGACG
    ATGGGCTCTC AGTGCCACAT CACCAAGACA ATGCAGGACA CCCTCAATCC AAAGTGGAAC
    TCCAACTGCC AGTTCTTTAT CAAAGACCTC GAGCAGGACG TGCTCTGCAT CACGGTGTTT
    GAGAGGGACC AGTTCTCCCC AGATGACTTT TTGGGCAGAA CAGAGATCCG TGTGGCAGAC
    ATTAAGAAGG ATCAGGGTTC AAAGGGACCA GTTACTAAGT GTCTCCTTCT CCATGAAGTC
    CCAACAGGAG AGATAGTCGT CCGACTAGAC CTGCAATTGT TTGATGAGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa02710
    Clone ID Related Accession (Same CDS sequence) XM_004934531.2 , XM_004934531.3
    Accession Version XM_004934531.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5136bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product intersectin-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455737.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004934531.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GGAGCTGAGA GAAATACATA ACAGACAGCA GCTTCAGAAA
    CAAAAGAACT TGGAAGCTGA GAGGCTGAAA CAGAAAGAAC AAGAAAGGAA GACAGAACTG
    GAAAAGCAAA AAGAAGCTCA AAGGCGAATC CAGGATCGGG ATAAACAACG GCTTGATCGA
    GTGCAACAAG AAGAAGAACC TCAATGGCAA AAAAAAAATC AAGAAGATGA CAAGCAAAAA
    AGGGAAGAAA TAATCAAGAA GAAGGAAAGT GAGGATAAGG GGAAACAGGA AATCCAAGAG
    AAACCGAGTA AGCTTTTCCA ACCACATCAA GAACCAGTTA AGCCAGCTGT CCAGGCTCCA
    TGGTCAAATG CAGGAAAAGC TCCACTAACA ATTTCTGCTC AGGAGGATGT AAAAATAGTG
    TATTATAGAG CACTGTATCC TTTTGAATCA AGAAGCCATG ACGAAATCAC TATTCAACCT
    GGCGACATAG TCATGGTGGA TGAAAGTCAG ACTGGAGAAC CAGGGTGGCT TGGTGGAGAA
    TTGAAGGGAA AAACTGGATG GTTCCCAGCA AACTACGCAG AAAAAATACC TGAAAGTGAA
    GTTCCAGCAT CTATAAAACC AGTTGAGGCA GCTCCTGCAC CTAAAGTTTC TGTGCATGAA
    ACAACCACAT CACTAGGAAC TGCTGCATCT ACAGAGTGCA CGACAACTGC TAACAACTGG
    GCAGACTTCA GTTCAACATG GCCAGCAAAC ACTAATGAGA AACCAGAGAC AGACAACTGG
    GATGCCTGGG CAGCTCAACC ATCTTTGACT GTTCCCAGTG CAGGGCAGCT GCGGCAGCGA
    TCAGCCTTCA CCCCCGCCAC TGTTACAGGA TCGTCTCCCT CTCCTGTATT GGGTCAGGGT
    GAAAAAGTGG AGGGACTTCA AGCACAGGCT CTGTATCCTT GGAGAGCAAA AAAAGACAAC
    CACCTAAATT TCAACAAAAA TGATGTCATC ACAGTTTTAG AGCAGCAAGA TATGTGGTGG
    TTTGGAGAAG TTCAAGGACA AAAGGGGTGG TTTCCTAAGT CATATGTGAA GCTTATTTCA
    GGCCCCATTA GGAAGTCAAC AAGCATGGAT TCTGGTTCTT CAGAAAGTCC TGCTAGTCTG
    AAAAGAGTAG CATCACCAGC AGCTAAAGCA ACTATGTCAG GTGAAGAATA TGTTGCTATG
    TATACTTATG AGAGTTCTGA ACAAGGAGAC CTAACCTTTC AGCAGGGTGA TATGATTCTT
    GTGACAAAGA AAGATGGCGA CTGGTGGACA GGAACACTGG GTGATAAAAC AGGAGTTTTC
    CCATCAAATT ATGTGAGACT CAAAGATTCT GAGGCTTCTG GAGCAGCTGG AAAAACAGGA
    AGCTTAGGGA AGAAACCTGA AATTGCCCAA GTTATTGCCT CTTACACAGC AACGGGTCCA
    GAACAACTCA CCCTTGCTCC TGGTCAGTTG ATTCTTATCA GAAAGAAGAA CCCAGGTGGC
    TGGTGGGAAG GAGAGCTCCA AGCACGAGGG AAAAAACGAC AAATAGGATG GTTTCCTGCT
    AATTATGTGA AACTTTTGAG CCCTGGTACC AGTAAAACTA CACCAACCGA GCTACCTAAA
    TCAACAGCAT TACCTTCAGT GTGCCAAGTA ATTGGCATGT ATGACTACAC TGCACAGAAT
    GATGATGAGC TAGCATTCAA TAAAGGCCAG ATTATAAACG TCCTTAACAA GGAAGACCCA
    GACTGGTGGA AAGGGGAGGT GAATGGACAA GTGGGTCTCT TCCCATCCAA CTATGTGAAG
    CTGACAACAG ATATGGACCC AAGCCAGCAA TGGTGTGCAG ACTTGCATCT TCTGGATATG
    CTGACACCTA CTGAACGAAA AAGGCAGGGA TACATCCATG AGCTCATTGT CACAGAAGAG
    AACTACGTAA ATGATCTGCA GTTGGTCACA GAGATCTTCC AGAAACCACT AATGGAATCT
    GAGCTGCTAA CAGAAAAGGA AGTTGCTATG ATTTTTGTTA ATTGGAAGGA GCTGATTATG
    TGCAATATAA AACTACTGAA GGCCTTGCGT GTGCGTAAGA AGATGTCTGG GGAGAAGATG
    CCAGTGAAAA TGATTGGTGA CATCCTGACA GCTCAGCTGC CACACATGCA GCCATACATT
    CGGTTCTGTA GCTGCCAGCT CAATGGGGCA GCACTCATTC AGCAGAAAAC AGATGAAGTC
    CCCGAATTCA AGGAGTTTGT AAAAAGGTTA GCAATGGATC CTCGCTGTAA AGGAATGCCA
    CTATCAAGTT TTCTACTAAA GCCTATGCAA CGGGTAACAA GATACCCACT AATAATTAAA
    AATATAATAG AAAACACTCC TGAAAACCAT CCTGACCACA GTCACTTGAA GCATGCTCTT
    GAAAAAGCAG AAGAATTATG TTCTCAAGTA AATGAAGGAG TGCGAGAGAA AGAAAATTCA
    GATAGGCTGG AGTGGATCCA GGCTCATGTT CAGTGCGAAG GCCTCTCTGA GCAACTCGTA
    TTTAATTCTG TTACAAACTG CTTGGGACCA CGCAAGTTTC TGCACAGTGG GAAGCTCTAT
    AAAGCCAAGA GTAACAAGGA ACTGTATGGC TTTCTGTTCA ACGATTTCCT CCTCTTGACT
    CAGATCATAA AACCTCTTGG CTCTTCTGGC ACAGACAAGG TCTTCAGCCC CAAGTCTAAT
    CTGCAGTACA AAATGTACAA AACTCCCATT TTTCTAAATG AAGTACTAGT AAAATTACCC
    ACAGACCCTT CTGGAGATGA GCCCATTTTC CATATCTCCC ACATTGACAG AGTCTACACA
    CTCCGGGCTG AAAGCATTAA TGAAAGGACT GCGTGGGTTC AGAAAATTAA AGCTGCTTCG
    GAACTTTACA TAGAGACTGA AAAGAAGAAG AGGGAAAAGG CCTACTTAGT TCGCTCACAA
    AGAGCAACAG GCATCGGGAG GTTGATGGTG AATATTGTTG AAGGCATTGA ACTAAAACCT
    TGCAGATCCC ATGGAAAGAG TAATCCATAC TGTGAGGTGA CGATGGGCTC TCAGTGCCAC
    ATCACCAAGA CAATGCAGGA CACCCTCAAT CCAAAGTGGA ACTCCAACTG CCAGTTCTTT
    ATCAAAGACC TCGAGCAGGA CGTGCTCTGC ATCACGGTGT TTGAGAGGGA CCAGTTCTCC
    CCAGATGACT TTTTGGGCAG AACAGAGATC CGTGTGGCAG ACATTAAGAA GGATCAGGGT
    TCAAAGGGAC CAGTTACTAA GTGTCTCCTT CTCCATGAAG TCCCAACAGG AGAGATAGTC
    GTCCGACTAG ACCTGCAATT GTTTGATGAG CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004934588.1
    CDS213..5348
    Translation

    Target ORF information:

    RefSeq Version XM_004934531.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004934531.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GGAGCTGAGA GAAATACATA ACAGACAGCA GCTTCAGAAA
    CAAAAGAACT TGGAAGCTGA GAGGCTGAAA CAGAAAGAAC AAGAAAGGAA GACAGAACTG
    GAAAAGCAAA AAGAAGCTCA AAGGCGAATC CAGGATCGGG ATAAACAACG GCTTGATCGA
    GTGCAACAAG AAGAAGAACC TCAATGGCAA AAAAAAAATC AAGAAGATGA CAAGCAAAAA
    AGGGAAGAAA TAATCAAGAA GAAGGAAAGT GAGGATAAGG GGAAACAGGA AATCCAAGAG
    AAACCGAGTA AGCTTTTCCA ACCACATCAA GAACCAGTTA AGCCAGCTGT CCAGGCTCCA
    TGGTCAAATG CAGGAAAAGC TCCACTAACA ATTTCTGCTC AGGAGGATGT AAAAATAGTG
    TATTATAGAG CACTGTATCC TTTTGAATCA AGAAGCCATG ACGAAATCAC TATTCAACCT
    GGCGACATAG TCATGGTGGA TGAAAGTCAG ACTGGAGAAC CAGGGTGGCT TGGTGGAGAA
    TTGAAGGGAA AAACTGGATG GTTCCCAGCA AACTACGCAG AAAAAATACC TGAAAGTGAA
    GTTCCAGCAT CTATAAAACC AGTTGAGGCA GCTCCTGCAC CTAAAGTTTC TGTGCATGAA
    ACAACCACAT CACTAGGAAC TGCTGCATCT ACAGAGTGCA CGACAACTGC TAACAACTGG
    GCAGACTTCA GTTCAACATG GCCAGCAAAC ACTAATGAGA AACCAGAGAC AGACAACTGG
    GATGCCTGGG CAGCTCAACC ATCTTTGACT GTTCCCAGTG CAGGGCAGCT GCGGCAGCGA
    TCAGCCTTCA CCCCCGCCAC TGTTACAGGA TCGTCTCCCT CTCCTGTATT GGGTCAGGGT
    GAAAAAGTGG AGGGACTTCA AGCACAGGCT CTGTATCCTT GGAGAGCAAA AAAAGACAAC
    CACCTAAATT TCAACAAAAA TGATGTCATC ACAGTTTTAG AGCAGCAAGA TATGTGGTGG
    TTTGGAGAAG TTCAAGGACA AAAGGGGTGG TTTCCTAAGT CATATGTGAA GCTTATTTCA
    GGCCCCATTA GGAAGTCAAC AAGCATGGAT TCTGGTTCTT CAGAAAGTCC TGCTAGTCTG
    AAAAGAGTAG CATCACCAGC AGCTAAAGCA ACTATGTCAG GTGAAGAATA TGTTGCTATG
    TATACTTATG AGAGTTCTGA ACAAGGAGAC CTAACCTTTC AGCAGGGTGA TATGATTCTT
    GTGACAAAGA AAGATGGCGA CTGGTGGACA GGAACACTGG GTGATAAAAC AGGAGTTTTC
    CCATCAAATT ATGTGAGACT CAAAGATTCT GAGGCTTCTG GAGCAGCTGG AAAAACAGGA
    AGCTTAGGGA AGAAACCTGA AATTGCCCAA GTTATTGCCT CTTACACAGC AACGGGTCCA
    GAACAACTCA CCCTTGCTCC TGGTCAGTTG ATTCTTATCA GAAAGAAGAA CCCAGGTGGC
    TGGTGGGAAG GAGAGCTCCA AGCACGAGGG AAAAAACGAC AAATAGGATG GTTTCCTGCT
    AATTATGTGA AACTTTTGAG CCCTGGTACC AGTAAAACTA CACCAACCGA GCTACCTAAA
    TCAACAGCAT TACCTTCAGT GTGCCAAGTA ATTGGCATGT ATGACTACAC TGCACAGAAT
    GATGATGAGC TAGCATTCAA TAAAGGCCAG ATTATAAACG TCCTTAACAA GGAAGACCCA
    GACTGGTGGA AAGGGGAGGT GAATGGACAA GTGGGTCTCT TCCCATCCAA CTATGTGAAG
    CTGACAACAG ATATGGACCC AAGCCAGCAA TGGTGTGCAG ACTTGCATCT TCTGGATATG
    CTGACACCTA CTGAACGAAA AAGGCAGGGA TACATCCATG AGCTCATTGT CACAGAAGAG
    AACTACGTAA ATGATCTGCA GTTGGTCACA GAGATCTTCC AGAAACCACT AATGGAATCT
    GAGCTGCTAA CAGAAAAGGA AGTTGCTATG ATTTTTGTTA ATTGGAAGGA GCTGATTATG
    TGCAATATAA AACTACTGAA GGCCTTGCGT GTGCGTAAGA AGATGTCTGG GGAGAAGATG
    CCAGTGAAAA TGATTGGTGA CATCCTGACA GCTCAGCTGC CACACATGCA GCCATACATT
    CGGTTCTGTA GCTGCCAGCT CAATGGGGCA GCACTCATTC AGCAGAAAAC AGATGAAGTC
    CCCGAATTCA AGGAGTTTGT AAAAAGGTTA GCAATGGATC CTCGCTGTAA AGGAATGCCA
    CTATCAAGTT TTCTACTAAA GCCTATGCAA CGGGTAACAA GATACCCACT AATAATTAAA
    AATATAATAG AAAACACTCC TGAAAACCAT CCTGACCACA GTCACTTGAA GCATGCTCTT
    GAAAAAGCAG AAGAATTATG TTCTCAAGTA AATGAAGGAG TGCGAGAGAA AGAAAATTCA
    GATAGGCTGG AGTGGATCCA GGCTCATGTT CAGTGCGAAG GCCTCTCTGA GCAACTCGTA
    TTTAATTCTG TTACAAACTG CTTGGGACCA CGCAAGTTTC TGCACAGTGG GAAGCTCTAT
    AAAGCCAAGA GTAACAAGGA ACTGTATGGC TTTCTGTTCA ACGATTTCCT CCTCTTGACT
    CAGATCATAA AACCTCTTGG CTCTTCTGGC ACAGACAAGG TCTTCAGCCC CAAGTCTAAT
    CTGCAGTACA AAATGTACAA AACTCCCATT TTTCTAAATG AAGTACTAGT AAAATTACCC
    ACAGACCCTT CTGGAGATGA GCCCATTTTC CATATCTCCC ACATTGACAG AGTCTACACA
    CTCCGGGCTG AAAGCATTAA TGAAAGGACT GCGTGGGTTC AGAAAATTAA AGCTGCTTCG
    GAACTTTACA TAGAGACTGA AAAGAAGAAG AGGGAAAAGG CCTACTTAGT TCGCTCACAA
    AGAGCAACAG GCATCGGGAG GTTGATGGTG AATATTGTTG AAGGCATTGA ACTAAAACCT
    TGCAGATCCC ATGGAAAGAG TAATCCATAC TGTGAGGTGA CGATGGGCTC TCAGTGCCAC
    ATCACCAAGA CAATGCAGGA CACCCTCAAT CCAAAGTGGA ACTCCAACTG CCAGTTCTTT
    ATCAAAGACC TCGAGCAGGA CGTGCTCTGC ATCACGGTGT TTGAGAGGGA CCAGTTCTCC
    CCAGATGACT TTTTGGGCAG AACAGAGATC CGTGTGGCAG ACATTAAGAA GGATCAGGGT
    TCAAAGGGAC CAGTTACTAA GTGTCTCCTT CTCCATGAAG TCCCAACAGG AGAGATAGTC
    GTCCGACTAG ACCTGCAATT GTTTGATGAG CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa02710
    Clone ID Related Accession (Same CDS sequence) XM_004934531.2 , XM_004934531.3
    Accession Version XM_004934531.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5136bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product intersectin-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004934531.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GGAGCTGAGA GAAATACATA ACAGACAGCA GCTTCAGAAA
    CAAAAGAACT TGGAAGCTGA GAGGCTGAAA CAGAAAGAAC AAGAAAGGAA GACAGAACTG
    GAAAAGCAAA AAGAAGCTCA AAGGCGAATC CAGGATCGGG ATAAACAACG GCTTGATCGA
    GTGCAACAAG AAGAAGAACC TCAATGGCAA AAAAAAAATC AAGAAGATGA CAAGCAAAAA
    AGGGAAGAAA TAATCAAGAA GAAGGAAAGT GAGGATAAGG GGAAACAGGA AATCCAAGAG
    AAACCGAGTA AGCTTTTCCA ACCACATCAA GAACCAGTTA AGCCAGCTGT CCAGGCTCCA
    TGGTCAAATG CAGGAAAAGC TCCACTAACA ATTTCTGCTC AGGAGGATGT AAAAATAGTG
    TATTATAGAG CACTGTATCC TTTTGAATCA AGAAGCCATG ACGAAATCAC TATTCAACCT
    GGCGACATAG TCATGGTGGA TGAAAGTCAG ACTGGAGAAC CAGGGTGGCT TGGTGGAGAA
    TTGAAGGGAA AAACTGGATG GTTCCCAGCA AACTACGCAG AAAAAATACC TGAAAGTGAA
    GTTCCAGCAT CTATAAAACC AGTTGAGGCA GCTCCTGCAC CTAAAGTTTC TGTGCATGAA
    ACAACCACAT CACTAGGAAC TGCTGCATCT ACAGAGTGCA CGACAACTGC TAACAACTGG
    GCAGACTTCA GTTCAACATG GCCAGCAAAC ACTAATGAGA AACCAGAGAC AGACAACTGG
    GATGCCTGGG CAGCTCAACC ATCTTTGACT GTTCCCAGTG CAGGGCAGCT GCGGCAGCGA
    TCAGCCTTCA CCCCCGCCAC TGTTACAGGA TCGTCTCCCT CTCCTGTATT GGGTCAGGGT
    GAAAAAGTGG AGGGACTTCA AGCACAGGCT CTGTATCCTT GGAGAGCAAA AAAAGACAAC
    CACCTAAATT TCAACAAAAA TGATGTCATC ACAGTTTTAG AGCAGCAAGA TATGTGGTGG
    TTTGGAGAAG TTCAAGGACA AAAGGGGTGG TTTCCTAAGT CATATGTGAA GCTTATTTCA
    GGCCCCATTA GGAAGTCAAC AAGCATGGAT TCTGGTTCTT CAGAAAGTCC TGCTAGTCTG
    AAAAGAGTAG CATCACCAGC AGCTAAAGCA ACTATGTCAG GTGAAGAATA TGTTGCTATG
    TATACTTATG AGAGTTCTGA ACAAGGAGAC CTAACCTTTC AGCAGGGTGA TATGATTCTT
    GTGACAAAGA AAGATGGCGA CTGGTGGACA GGAACACTGG GTGATAAAAC AGGAGTTTTC
    CCATCAAATT ATGTGAGACT CAAAGATTCT GAGGCTTCTG GAGCAGCTGG AAAAACAGGA
    AGCTTAGGGA AGAAACCTGA AATTGCCCAA GTTATTGCCT CTTACACAGC AACGGGTCCA
    GAACAACTCA CCCTTGCTCC TGGTCAGTTG ATTCTTATCA GAAAGAAGAA CCCAGGTGGC
    TGGTGGGAAG GAGAGCTCCA AGCACGAGGG AAAAAACGAC AAATAGGATG GTTTCCTGCT
    AATTATGTGA AACTTTTGAG CCCTGGTACC AGTAAAACTA CACCAACCGA GCTACCTAAA
    TCAACAGCAT TACCTTCAGT GTGCCAAGTA ATTGGCATGT ATGACTACAC TGCACAGAAT
    GATGATGAGC TAGCATTCAA TAAAGGCCAG ATTATAAACG TCCTTAACAA GGAAGACCCA
    GACTGGTGGA AAGGGGAGGT GAATGGACAA GTGGGTCTCT TCCCATCCAA CTATGTGAAG
    CTGACAACAG ATATGGACCC AAGCCAGCAA TGGTGTGCAG ACTTGCATCT TCTGGATATG
    CTGACACCTA CTGAACGAAA AAGGCAGGGA TACATCCATG AGCTCATTGT CACAGAAGAG
    AACTACGTAA ATGATCTGCA GTTGGTCACA GAGATCTTCC AGAAACCACT AATGGAATCT
    GAGCTGCTAA CAGAAAAGGA AGTTGCTATG ATTTTTGTTA ATTGGAAGGA GCTGATTATG
    TGCAATATAA AACTACTGAA GGCCTTGCGT GTGCGTAAGA AGATGTCTGG GGAGAAGATG
    CCAGTGAAAA TGATTGGTGA CATCCTGACA GCTCAGCTGC CACACATGCA GCCATACATT
    CGGTTCTGTA GCTGCCAGCT CAATGGGGCA GCACTCATTC AGCAGAAAAC AGATGAAGTC
    CCCGAATTCA AGGAGTTTGT AAAAAGGTTA GCAATGGATC CTCGCTGTAA AGGAATGCCA
    CTATCAAGTT TTCTACTAAA GCCTATGCAA CGGGTAACAA GATACCCACT AATAATTAAA
    AATATAATAG AAAACACTCC TGAAAACCAT CCTGACCACA GTCACTTGAA GCATGCTCTT
    GAAAAAGCAG AAGAATTATG TTCTCAAGTA AATGAAGGAG TGCGAGAGAA AGAAAATTCA
    GATAGGCTGG AGTGGATCCA GGCTCATGTT CAGTGCGAAG GCCTCTCTGA GCAACTCGTA
    TTTAATTCTG TTACAAACTG CTTGGGACCA CGCAAGTTTC TGCACAGTGG GAAGCTCTAT
    AAAGCCAAGA GTAACAAGGA ACTGTATGGC TTTCTGTTCA ACGATTTCCT CCTCTTGACT
    CAGATCATAA AACCTCTTGG CTCTTCTGGC ACAGACAAGG TCTTCAGCCC CAAGTCTAAT
    CTGCAGTACA AAATGTACAA AACTCCCATT TTTCTAAATG AAGTACTAGT AAAATTACCC
    ACAGACCCTT CTGGAGATGA GCCCATTTTC CATATCTCCC ACATTGACAG AGTCTACACA
    CTCCGGGCTG AAAGCATTAA TGAAAGGACT GCGTGGGTTC AGAAAATTAA AGCTGCTTCG
    GAACTTTACA TAGAGACTGA AAAGAAGAAG AGGGAAAAGG CCTACTTAGT TCGCTCACAA
    AGAGCAACAG GCATCGGGAG GTTGATGGTG AATATTGTTG AAGGCATTGA ACTAAAACCT
    TGCAGATCCC ATGGAAAGAG TAATCCATAC TGTGAGGTGA CGATGGGCTC TCAGTGCCAC
    ATCACCAAGA CAATGCAGGA CACCCTCAAT CCAAAGTGGA ACTCCAACTG CCAGTTCTTT
    ATCAAAGACC TCGAGCAGGA CGTGCTCTGC ATCACGGTGT TTGAGAGGGA CCAGTTCTCC
    CCAGATGACT TTTTGGGCAG AACAGAGATC CGTGTGGCAG ACATTAAGAA GGATCAGGGT
    TCAAAGGGAC CAGTTACTAA GTGTCTCCTT CTCCATGAAG TCCCAACAGG AGAGATAGTC
    GTCCGACTAG ACCTGCAATT GTTTGATGAG CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004934588.1
    CDS230..5365
    Translation

    Target ORF information:

    RefSeq Version XM_004934531.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus intersectin 1 (ITSN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004934531.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GGAGCTGAGA GAAATACATA ACAGACAGCA GCTTCAGAAA
    CAAAAGAACT TGGAAGCTGA GAGGCTGAAA CAGAAAGAAC AAGAAAGGAA GACAGAACTG
    GAAAAGCAAA AAGAAGCTCA AAGGCGAATC CAGGATCGGG ATAAACAACG GCTTGATCGA
    GTGCAACAAG AAGAAGAACC TCAATGGCAA AAAAAAAATC AAGAAGATGA CAAGCAAAAA
    AGGGAAGAAA TAATCAAGAA GAAGGAAAGT GAGGATAAGG GGAAACAGGA AATCCAAGAG
    AAACCGAGTA AGCTTTTCCA ACCACATCAA GAACCAGTTA AGCCAGCTGT CCAGGCTCCA
    TGGTCAAATG CAGGAAAAGC TCCACTAACA ATTTCTGCTC AGGAGGATGT AAAAATAGTG
    TATTATAGAG CACTGTATCC TTTTGAATCA AGAAGCCATG ACGAAATCAC TATTCAACCT
    GGCGACATAG TCATGGTGGA TGAAAGTCAG ACTGGAGAAC CAGGGTGGCT TGGTGGAGAA
    TTGAAGGGAA AAACTGGATG GTTCCCAGCA AACTACGCAG AAAAAATACC TGAAAGTGAA
    GTTCCAGCAT CTATAAAACC AGTTGAGGCA GCTCCTGCAC CTAAAGTTTC TGTGCATGAA
    ACAACCACAT CACTAGGAAC TGCTGCATCT ACAGAGTGCA CGACAACTGC TAACAACTGG
    GCAGACTTCA GTTCAACATG GCCAGCAAAC ACTAATGAGA AACCAGAGAC AGACAACTGG
    GATGCCTGGG CAGCTCAACC ATCTTTGACT GTTCCCAGTG CAGGGCAGCT GCGGCAGCGA
    TCAGCCTTCA CCCCCGCCAC TGTTACAGGA TCGTCTCCCT CTCCTGTATT GGGTCAGGGT
    GAAAAAGTGG AGGGACTTCA AGCACAGGCT CTGTATCCTT GGAGAGCAAA AAAAGACAAC
    CACCTAAATT TCAACAAAAA TGATGTCATC ACAGTTTTAG AGCAGCAAGA TATGTGGTGG
    TTTGGAGAAG TTCAAGGACA AAAGGGGTGG TTTCCTAAGT CATATGTGAA GCTTATTTCA
    GGCCCCATTA GGAAGTCAAC AAGCATGGAT TCTGGTTCTT CAGAAAGTCC TGCTAGTCTG
    AAAAGAGTAG CATCACCAGC AGCTAAAGCA ACTATGTCAG GTGAAGAATA TGTTGCTATG
    TATACTTATG AGAGTTCTGA ACAAGGAGAC CTAACCTTTC AGCAGGGTGA TATGATTCTT
    GTGACAAAGA AAGATGGCGA CTGGTGGACA GGAACACTGG GTGATAAAAC AGGAGTTTTC
    CCATCAAATT ATGTGAGACT CAAAGATTCT GAGGCTTCTG GAGCAGCTGG AAAAACAGGA
    AGCTTAGGGA AGAAACCTGA AATTGCCCAA GTTATTGCCT CTTACACAGC AACGGGTCCA
    GAACAACTCA CCCTTGCTCC TGGTCAGTTG ATTCTTATCA GAAAGAAGAA CCCAGGTGGC
    TGGTGGGAAG GAGAGCTCCA AGCACGAGGG AAAAAACGAC AAATAGGATG GTTTCCTGCT
    AATTATGTGA AACTTTTGAG CCCTGGTACC AGTAAAACTA CACCAACCGA GCTACCTAAA
    TCAACAGCAT TACCTTCAGT GTGCCAAGTA ATTGGCATGT ATGACTACAC TGCACAGAAT
    GATGATGAGC TAGCATTCAA TAAAGGCCAG ATTATAAACG TCCTTAACAA GGAAGACCCA
    GACTGGTGGA AAGGGGAGGT GAATGGACAA GTGGGTCTCT TCCCATCCAA CTATGTGAAG
    CTGACAACAG ATATGGACCC AAGCCAGCAA TGGTGTGCAG ACTTGCATCT TCTGGATATG
    CTGACACCTA CTGAACGAAA AAGGCAGGGA TACATCCATG AGCTCATTGT CACAGAAGAG
    AACTACGTAA ATGATCTGCA GTTGGTCACA GAGATCTTCC AGAAACCACT AATGGAATCT
    GAGCTGCTAA CAGAAAAGGA AGTTGCTATG ATTTTTGTTA ATTGGAAGGA GCTGATTATG
    TGCAATATAA AACTACTGAA GGCCTTGCGT GTGCGTAAGA AGATGTCTGG GGAGAAGATG
    CCAGTGAAAA TGATTGGTGA CATCCTGACA GCTCAGCTGC CACACATGCA GCCATACATT
    CGGTTCTGTA GCTGCCAGCT CAATGGGGCA GCACTCATTC AGCAGAAAAC AGATGAAGTC
    CCCGAATTCA AGGAGTTTGT AAAAAGGTTA GCAATGGATC CTCGCTGTAA AGGAATGCCA
    CTATCAAGTT TTCTACTAAA GCCTATGCAA CGGGTAACAA GATACCCACT AATAATTAAA
    AATATAATAG AAAACACTCC TGAAAACCAT CCTGACCACA GTCACTTGAA GCATGCTCTT
    GAAAAAGCAG AAGAATTATG TTCTCAAGTA AATGAAGGAG TGCGAGAGAA AGAAAATTCA
    GATAGGCTGG AGTGGATCCA GGCTCATGTT CAGTGCGAAG GCCTCTCTGA GCAACTCGTA
    TTTAATTCTG TTACAAACTG CTTGGGACCA CGCAAGTTTC TGCACAGTGG GAAGCTCTAT
    AAAGCCAAGA GTAACAAGGA ACTGTATGGC TTTCTGTTCA ACGATTTCCT CCTCTTGACT
    CAGATCATAA AACCTCTTGG CTCTTCTGGC ACAGACAAGG TCTTCAGCCC CAAGTCTAAT
    CTGCAGTACA AAATGTACAA AACTCCCATT TTTCTAAATG AAGTACTAGT AAAATTACCC
    ACAGACCCTT CTGGAGATGA GCCCATTTTC CATATCTCCC ACATTGACAG AGTCTACACA
    CTCCGGGCTG AAAGCATTAA TGAAAGGACT GCGTGGGTTC AGAAAATTAA AGCTGCTTCG
    GAACTTTACA TAGAGACTGA AAAGAAGAAG AGGGAAAAGG CCTACTTAGT TCGCTCACAA
    AGAGCAACAG GCATCGGGAG GTTGATGGTG AATATTGTTG AAGGCATTGA ACTAAAACCT
    TGCAGATCCC ATGGAAAGAG TAATCCATAC TGTGAGGTGA CGATGGGCTC TCAGTGCCAC
    ATCACCAAGA CAATGCAGGA CACCCTCAAT CCAAAGTGGA ACTCCAACTG CCAGTTCTTT
    ATCAAAGACC TCGAGCAGGA CGTGCTCTGC ATCACGGTGT TTGAGAGGGA CCAGTTCTCC
    CCAGATGACT TTTTGGGCAG AACAGAGATC CGTGTGGCAG ACATTAAGAA GGATCAGGGT
    TCAAAGGGAC CAGTTACTAA GTGTCTCCTT CTCCATGAAG TCCCAACAGG AGAGATAGTC
    GTCCGACTAG ACCTGCAATT GTTTGATGAG CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa02711
    Clone ID Related Accession (Same CDS sequence) XM_416715.5 , XM_416715.6
    Accession Version XM_416715.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product intersectin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455737.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_416715.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GGAGCTGAGA GAAATACATA ACAGACAGCA GCTTCAGAAA
    CAAAAGAACT TGGAAGCTGA GAGGCTGAAA CAGAAAGAAC AAGAAAGGAA GACAGAACTG
    GAAAAGCAAA AAGAAGCTCA AAGGCGAATC CAGGATCGGG ATAAACAACG GCTTGATCGA
    GTGCAACAAG AAGAAGAACC TCAATGGCAA AAAAAAAATC AAGAAGATGA CAAGCAAAAA
    AGGGAAGAAA TAATCAAGAA GAAGGAAAGT GAGGATAAGG GGAAACAGGA AATCCAAGAG
    AAACCGAGTA AGCTTTTCCA ACCACATCAA GAACCAGTTA AGCCAGCTGT CCAGGCTCCA
    TGGTCAAATG CAGGAAAAGC TCCACTAACA ATTTCTGCTC AGGAGGATGT AAAAATAGTG
    TATTATAGAG CACTGTATCC TTTTGAATCA AGAAGCCATG ACGAAATCAC TATTCAACCT
    GGCGACATAG TCATGGTTAA AAGGGAATGG GTGGATGAAA GTCAGACTGG AGAACCAGGG
    TGGCTTGGTG GAGAATTGAA GGGAAAAACT GGATGGTTCC CAGCAAACTA CGCAGAAAAA
    ATACCTGAAA GTGAAGTTCC AGCATCTATA AAACCAGTTG AGGCAGCTCC TGCACCTAAA
    GTTTCTGTGC ATGAAACAAC CACATCACTA GGAACTGCTG CATCTACAGA GTGCACGACA
    ACTGCTAACA ACTGGGCAGA CTTCAGTTCA ACATGGCCAG CAAACACTAA TGAGAAACCA
    GAGACAGACA ACTGGGATGC CTGGGCAGCT CAACCATCTT TGACTGTTCC CAGTGCAGGG
    CAGCTGCGGC AGCGATCAGC CTTCACCCCC GCCACTGTTA CAGGATCGTC TCCCTCTCCT
    GTATTGGGTC AGGGTGAAAA AGTGGAGGGA CTTCAAGCAC AGGCTCTGTA TCCTTGGAGA
    GCAAAAAAAG ACAACCACCT AAATTTCAAC AAAAATGATG TCATCACAGT TTTAGAGCAG
    CAAGATATGT GGTGGTTTGG AGAAGTTCAA GGACAAAAGG GGTGGTTTCC TAAGTCATAT
    GTGAAGCTTA TTTCAGGCCC CATTAGGAAG TCAACAAGCA TGGATTCTGG TTCTTCAGAA
    AGTCCTGCTA GTCTGAAAAG AGTAGCATCA CCAGCAGCTA AAGCAACTAT GTCAGGTGAA
    GAATATGTTG CTATGTATAC TTATGAGAGT TCTGAACAAG GAGACCTAAC CTTTCAGCAG
    GGTGATATGA TTCTTGTGAC AAAGAAAGAT GGCGACTGGT GGACAGGAAC ACTGGGTGAT
    AAAACAGGAG TTTTCCCATC AAATTATGTG AGACTCAAAG ATTCTGAGGC TTCTGGAGCA
    GCTGGAAAAA CAGGAAGCTT AGGGAAGAAA CCTGAAATTG CCCAAGTTAT TGCCTCTTAC
    ACAGCAACGG GTCCAGAACA ACTCACCCTT GCTCCTGGTC AGTTGATTCT TATCAGAAAG
    AAGAACCCAG GTGGCTGGTG GGAAGGAGAG CTCCAAGCAC GAGGGAAAAA ACGACAAATA
    GGATGGTTTC CTGCTAATTA TGTGAAACTT TTGAGCCCTG GTACCAGTAA AACTACACCA
    ACCGAGCTAC CTAAATCAAC AGCATTACCT TCAGTGTGCC AAGTAATTGG CATGTATGAC
    TACACTGCAC AGAATGATGA TGAGCTAGCA TTCAATAAAG GCCAGATTAT AAACGTCCTT
    AACAAGGAAG ACCCAGACTG GTGGAAAGGG GAGGTGAATG GACAAGTGGG TCTCTTCCCA
    TCCAACTATG TGAAGCTGAC AACAGATATG GACCCAAGCC AGCAATGGTG TGCAGACTTG
    CATCTTCTGG ATATGCTGAC ACCTACTGAA CGAAAAAGGC AGGGATACAT CCATGAGCTC
    ATTGTCACAG AAGAGAACTA CGTAAATGAT CTGCAGTTGG TCACAGAGAT CTTCCAGAAA
    CCACTAATGG AATCTGAGCT GCTAACAGAA AAGGAAGTTG CTATGATTTT TGTTAATTGG
    AAGGAGCTGA TTATGTGCAA TATAAAACTA CTGAAGGCCT TGCGTGTGCG TAAGAAGATG
    TCTGGGGAGA AGATGCCAGT GAAAATGATT GGTGACATCC TGACAGCTCA GCTGCCACAC
    ATGCAGCCAT ACATTCGGTT CTGTAGCTGC CAGCTCAATG GGGCAGCACT CATTCAGCAG
    AAAACAGATG AAGTCCCCGA ATTCAAGGAG TTTGTAAAAA GGTTAGCAAT GGATCCTCGC
    TGTAAAGGAA TGCCACTATC AAGTTTTCTA CTAAAGCCTA TGCAACGGGT AACAAGATAC
    CCACTAATAA TTAAAAATAT AATAGAAAAC ACTCCTGAAA ACCATCCTGA CCACAGTCAC
    TTGAAGCATG CTCTTGAAAA AGCAGAAGAA TTATGTTCTC AAGTAAATGA AGGAGTGCGA
    GAGAAAGAAA ATTCAGATAG GCTGGAGTGG ATCCAGGCTC ATGTTCAGTG CGAAGGCCTC
    TCTGAGCAAC TCGTATTTAA TTCTGTTACA AACTGCTTGG GACCACGCAA GTTTCTGCAC
    AGTGGGAAGC TCTATAAAGC CAAGAGTAAC AAGGAACTGT ATGGCTTTCT GTTCAACGAT
    TTCCTCCTCT TGACTCAGAT CATAAAACCT CTTGGCTCTT CTGGCACAGA CAAGGTCTTC
    AGCCCCAAGT CTAATCTGCA GTACAAAATG TACAAAACTC CCATTTTTCT AAATGAAGTA
    CTAGTAAAAT TACCCACAGA CCCTTCTGGA GATGAGCCCA TTTTCCATAT CTCCCACATT
    GACAGAGTCT ACACACTCCG GGCTGAAAGC ATTAATGAAA GGACTGCGTG GGTTCAGAAA
    ATTAAAGCTG CTTCGGAACT TTACATAGAG ACTGAAAAGA AGAAGAGGGA AAAGGCCTAC
    TTAGTTCGCT CACAAAGAGC AACAGGCATC GGGAGGTTGA TGGTGAATAT TGTTGAAGGC
    ATTGAACTAA AACCTTGCAG ATCCCATGGA AAGAGTAATC CATACTGTGA GGTGACGATG
    GGCTCTCAGT GCCACATCAC CAAGACAATG CAGGACACCC TCAATCCAAA GTGGAACTCC
    AACTGCCAGT TCTTTATCAA AGACCTCGAG CAGGACGTGC TCTGCATCAC GGTGTTTGAG
    AGGGACCAGT TCTCCCCAGA TGACTTTTTG GGCAGAACAG AGATCCGTGT GGCAGACATT
    AAGAAGGATC AGGGTTCAAA GGGACCAGTT ACTAAGTGTC TCCTTCTCCA TGAAGTCCCA
    ACAGGAGAGA TAGTCGTCCG ACTAGACCTG CAATTGTTTG ATGAGCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_416715.2
    CDS213..5363
    Translation

    Target ORF information:

    RefSeq Version XM_416715.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_416715.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GGAGCTGAGA GAAATACATA ACAGACAGCA GCTTCAGAAA
    CAAAAGAACT TGGAAGCTGA GAGGCTGAAA CAGAAAGAAC AAGAAAGGAA GACAGAACTG
    GAAAAGCAAA AAGAAGCTCA AAGGCGAATC CAGGATCGGG ATAAACAACG GCTTGATCGA
    GTGCAACAAG AAGAAGAACC TCAATGGCAA AAAAAAAATC AAGAAGATGA CAAGCAAAAA
    AGGGAAGAAA TAATCAAGAA GAAGGAAAGT GAGGATAAGG GGAAACAGGA AATCCAAGAG
    AAACCGAGTA AGCTTTTCCA ACCACATCAA GAACCAGTTA AGCCAGCTGT CCAGGCTCCA
    TGGTCAAATG CAGGAAAAGC TCCACTAACA ATTTCTGCTC AGGAGGATGT AAAAATAGTG
    TATTATAGAG CACTGTATCC TTTTGAATCA AGAAGCCATG ACGAAATCAC TATTCAACCT
    GGCGACATAG TCATGGTTAA AAGGGAATGG GTGGATGAAA GTCAGACTGG AGAACCAGGG
    TGGCTTGGTG GAGAATTGAA GGGAAAAACT GGATGGTTCC CAGCAAACTA CGCAGAAAAA
    ATACCTGAAA GTGAAGTTCC AGCATCTATA AAACCAGTTG AGGCAGCTCC TGCACCTAAA
    GTTTCTGTGC ATGAAACAAC CACATCACTA GGAACTGCTG CATCTACAGA GTGCACGACA
    ACTGCTAACA ACTGGGCAGA CTTCAGTTCA ACATGGCCAG CAAACACTAA TGAGAAACCA
    GAGACAGACA ACTGGGATGC CTGGGCAGCT CAACCATCTT TGACTGTTCC CAGTGCAGGG
    CAGCTGCGGC AGCGATCAGC CTTCACCCCC GCCACTGTTA CAGGATCGTC TCCCTCTCCT
    GTATTGGGTC AGGGTGAAAA AGTGGAGGGA CTTCAAGCAC AGGCTCTGTA TCCTTGGAGA
    GCAAAAAAAG ACAACCACCT AAATTTCAAC AAAAATGATG TCATCACAGT TTTAGAGCAG
    CAAGATATGT GGTGGTTTGG AGAAGTTCAA GGACAAAAGG GGTGGTTTCC TAAGTCATAT
    GTGAAGCTTA TTTCAGGCCC CATTAGGAAG TCAACAAGCA TGGATTCTGG TTCTTCAGAA
    AGTCCTGCTA GTCTGAAAAG AGTAGCATCA CCAGCAGCTA AAGCAACTAT GTCAGGTGAA
    GAATATGTTG CTATGTATAC TTATGAGAGT TCTGAACAAG GAGACCTAAC CTTTCAGCAG
    GGTGATATGA TTCTTGTGAC AAAGAAAGAT GGCGACTGGT GGACAGGAAC ACTGGGTGAT
    AAAACAGGAG TTTTCCCATC AAATTATGTG AGACTCAAAG ATTCTGAGGC TTCTGGAGCA
    GCTGGAAAAA CAGGAAGCTT AGGGAAGAAA CCTGAAATTG CCCAAGTTAT TGCCTCTTAC
    ACAGCAACGG GTCCAGAACA ACTCACCCTT GCTCCTGGTC AGTTGATTCT TATCAGAAAG
    AAGAACCCAG GTGGCTGGTG GGAAGGAGAG CTCCAAGCAC GAGGGAAAAA ACGACAAATA
    GGATGGTTTC CTGCTAATTA TGTGAAACTT TTGAGCCCTG GTACCAGTAA AACTACACCA
    ACCGAGCTAC CTAAATCAAC AGCATTACCT TCAGTGTGCC AAGTAATTGG CATGTATGAC
    TACACTGCAC AGAATGATGA TGAGCTAGCA TTCAATAAAG GCCAGATTAT AAACGTCCTT
    AACAAGGAAG ACCCAGACTG GTGGAAAGGG GAGGTGAATG GACAAGTGGG TCTCTTCCCA
    TCCAACTATG TGAAGCTGAC AACAGATATG GACCCAAGCC AGCAATGGTG TGCAGACTTG
    CATCTTCTGG ATATGCTGAC ACCTACTGAA CGAAAAAGGC AGGGATACAT CCATGAGCTC
    ATTGTCACAG AAGAGAACTA CGTAAATGAT CTGCAGTTGG TCACAGAGAT CTTCCAGAAA
    CCACTAATGG AATCTGAGCT GCTAACAGAA AAGGAAGTTG CTATGATTTT TGTTAATTGG
    AAGGAGCTGA TTATGTGCAA TATAAAACTA CTGAAGGCCT TGCGTGTGCG TAAGAAGATG
    TCTGGGGAGA AGATGCCAGT GAAAATGATT GGTGACATCC TGACAGCTCA GCTGCCACAC
    ATGCAGCCAT ACATTCGGTT CTGTAGCTGC CAGCTCAATG GGGCAGCACT CATTCAGCAG
    AAAACAGATG AAGTCCCCGA ATTCAAGGAG TTTGTAAAAA GGTTAGCAAT GGATCCTCGC
    TGTAAAGGAA TGCCACTATC AAGTTTTCTA CTAAAGCCTA TGCAACGGGT AACAAGATAC
    CCACTAATAA TTAAAAATAT AATAGAAAAC ACTCCTGAAA ACCATCCTGA CCACAGTCAC
    TTGAAGCATG CTCTTGAAAA AGCAGAAGAA TTATGTTCTC AAGTAAATGA AGGAGTGCGA
    GAGAAAGAAA ATTCAGATAG GCTGGAGTGG ATCCAGGCTC ATGTTCAGTG CGAAGGCCTC
    TCTGAGCAAC TCGTATTTAA TTCTGTTACA AACTGCTTGG GACCACGCAA GTTTCTGCAC
    AGTGGGAAGC TCTATAAAGC CAAGAGTAAC AAGGAACTGT ATGGCTTTCT GTTCAACGAT
    TTCCTCCTCT TGACTCAGAT CATAAAACCT CTTGGCTCTT CTGGCACAGA CAAGGTCTTC
    AGCCCCAAGT CTAATCTGCA GTACAAAATG TACAAAACTC CCATTTTTCT AAATGAAGTA
    CTAGTAAAAT TACCCACAGA CCCTTCTGGA GATGAGCCCA TTTTCCATAT CTCCCACATT
    GACAGAGTCT ACACACTCCG GGCTGAAAGC ATTAATGAAA GGACTGCGTG GGTTCAGAAA
    ATTAAAGCTG CTTCGGAACT TTACATAGAG ACTGAAAAGA AGAAGAGGGA AAAGGCCTAC
    TTAGTTCGCT CACAAAGAGC AACAGGCATC GGGAGGTTGA TGGTGAATAT TGTTGAAGGC
    ATTGAACTAA AACCTTGCAG ATCCCATGGA AAGAGTAATC CATACTGTGA GGTGACGATG
    GGCTCTCAGT GCCACATCAC CAAGACAATG CAGGACACCC TCAATCCAAA GTGGAACTCC
    AACTGCCAGT TCTTTATCAA AGACCTCGAG CAGGACGTGC TCTGCATCAC GGTGTTTGAG
    AGGGACCAGT TCTCCCCAGA TGACTTTTTG GGCAGAACAG AGATCCGTGT GGCAGACATT
    AAGAAGGATC AGGGTTCAAA GGGACCAGTT ACTAAGTGTC TCCTTCTCCA TGAAGTCCCA
    ACAGGAGAGA TAGTCGTCCG ACTAGACCTG CAATTGTTTG ATGAGCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa02711
    Clone ID Related Accession (Same CDS sequence) XM_416715.5 , XM_416715.6
    Accession Version XM_416715.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product intersectin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_416715.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GGAGCTGAGA GAAATACATA ACAGACAGCA GCTTCAGAAA
    CAAAAGAACT TGGAAGCTGA GAGGCTGAAA CAGAAAGAAC AAGAAAGGAA GACAGAACTG
    GAAAAGCAAA AAGAAGCTCA AAGGCGAATC CAGGATCGGG ATAAACAACG GCTTGATCGA
    GTGCAACAAG AAGAAGAACC TCAATGGCAA AAAAAAAATC AAGAAGATGA CAAGCAAAAA
    AGGGAAGAAA TAATCAAGAA GAAGGAAAGT GAGGATAAGG GGAAACAGGA AATCCAAGAG
    AAACCGAGTA AGCTTTTCCA ACCACATCAA GAACCAGTTA AGCCAGCTGT CCAGGCTCCA
    TGGTCAAATG CAGGAAAAGC TCCACTAACA ATTTCTGCTC AGGAGGATGT AAAAATAGTG
    TATTATAGAG CACTGTATCC TTTTGAATCA AGAAGCCATG ACGAAATCAC TATTCAACCT
    GGCGACATAG TCATGGTTAA AAGGGAATGG GTGGATGAAA GTCAGACTGG AGAACCAGGG
    TGGCTTGGTG GAGAATTGAA GGGAAAAACT GGATGGTTCC CAGCAAACTA CGCAGAAAAA
    ATACCTGAAA GTGAAGTTCC AGCATCTATA AAACCAGTTG AGGCAGCTCC TGCACCTAAA
    GTTTCTGTGC ATGAAACAAC CACATCACTA GGAACTGCTG CATCTACAGA GTGCACGACA
    ACTGCTAACA ACTGGGCAGA CTTCAGTTCA ACATGGCCAG CAAACACTAA TGAGAAACCA
    GAGACAGACA ACTGGGATGC CTGGGCAGCT CAACCATCTT TGACTGTTCC CAGTGCAGGG
    CAGCTGCGGC AGCGATCAGC CTTCACCCCC GCCACTGTTA CAGGATCGTC TCCCTCTCCT
    GTATTGGGTC AGGGTGAAAA AGTGGAGGGA CTTCAAGCAC AGGCTCTGTA TCCTTGGAGA
    GCAAAAAAAG ACAACCACCT AAATTTCAAC AAAAATGATG TCATCACAGT TTTAGAGCAG
    CAAGATATGT GGTGGTTTGG AGAAGTTCAA GGACAAAAGG GGTGGTTTCC TAAGTCATAT
    GTGAAGCTTA TTTCAGGCCC CATTAGGAAG TCAACAAGCA TGGATTCTGG TTCTTCAGAA
    AGTCCTGCTA GTCTGAAAAG AGTAGCATCA CCAGCAGCTA AAGCAACTAT GTCAGGTGAA
    GAATATGTTG CTATGTATAC TTATGAGAGT TCTGAACAAG GAGACCTAAC CTTTCAGCAG
    GGTGATATGA TTCTTGTGAC AAAGAAAGAT GGCGACTGGT GGACAGGAAC ACTGGGTGAT
    AAAACAGGAG TTTTCCCATC AAATTATGTG AGACTCAAAG ATTCTGAGGC TTCTGGAGCA
    GCTGGAAAAA CAGGAAGCTT AGGGAAGAAA CCTGAAATTG CCCAAGTTAT TGCCTCTTAC
    ACAGCAACGG GTCCAGAACA ACTCACCCTT GCTCCTGGTC AGTTGATTCT TATCAGAAAG
    AAGAACCCAG GTGGCTGGTG GGAAGGAGAG CTCCAAGCAC GAGGGAAAAA ACGACAAATA
    GGATGGTTTC CTGCTAATTA TGTGAAACTT TTGAGCCCTG GTACCAGTAA AACTACACCA
    ACCGAGCTAC CTAAATCAAC AGCATTACCT TCAGTGTGCC AAGTAATTGG CATGTATGAC
    TACACTGCAC AGAATGATGA TGAGCTAGCA TTCAATAAAG GCCAGATTAT AAACGTCCTT
    AACAAGGAAG ACCCAGACTG GTGGAAAGGG GAGGTGAATG GACAAGTGGG TCTCTTCCCA
    TCCAACTATG TGAAGCTGAC AACAGATATG GACCCAAGCC AGCAATGGTG TGCAGACTTG
    CATCTTCTGG ATATGCTGAC ACCTACTGAA CGAAAAAGGC AGGGATACAT CCATGAGCTC
    ATTGTCACAG AAGAGAACTA CGTAAATGAT CTGCAGTTGG TCACAGAGAT CTTCCAGAAA
    CCACTAATGG AATCTGAGCT GCTAACAGAA AAGGAAGTTG CTATGATTTT TGTTAATTGG
    AAGGAGCTGA TTATGTGCAA TATAAAACTA CTGAAGGCCT TGCGTGTGCG TAAGAAGATG
    TCTGGGGAGA AGATGCCAGT GAAAATGATT GGTGACATCC TGACAGCTCA GCTGCCACAC
    ATGCAGCCAT ACATTCGGTT CTGTAGCTGC CAGCTCAATG GGGCAGCACT CATTCAGCAG
    AAAACAGATG AAGTCCCCGA ATTCAAGGAG TTTGTAAAAA GGTTAGCAAT GGATCCTCGC
    TGTAAAGGAA TGCCACTATC AAGTTTTCTA CTAAAGCCTA TGCAACGGGT AACAAGATAC
    CCACTAATAA TTAAAAATAT AATAGAAAAC ACTCCTGAAA ACCATCCTGA CCACAGTCAC
    TTGAAGCATG CTCTTGAAAA AGCAGAAGAA TTATGTTCTC AAGTAAATGA AGGAGTGCGA
    GAGAAAGAAA ATTCAGATAG GCTGGAGTGG ATCCAGGCTC ATGTTCAGTG CGAAGGCCTC
    TCTGAGCAAC TCGTATTTAA TTCTGTTACA AACTGCTTGG GACCACGCAA GTTTCTGCAC
    AGTGGGAAGC TCTATAAAGC CAAGAGTAAC AAGGAACTGT ATGGCTTTCT GTTCAACGAT
    TTCCTCCTCT TGACTCAGAT CATAAAACCT CTTGGCTCTT CTGGCACAGA CAAGGTCTTC
    AGCCCCAAGT CTAATCTGCA GTACAAAATG TACAAAACTC CCATTTTTCT AAATGAAGTA
    CTAGTAAAAT TACCCACAGA CCCTTCTGGA GATGAGCCCA TTTTCCATAT CTCCCACATT
    GACAGAGTCT ACACACTCCG GGCTGAAAGC ATTAATGAAA GGACTGCGTG GGTTCAGAAA
    ATTAAAGCTG CTTCGGAACT TTACATAGAG ACTGAAAAGA AGAAGAGGGA AAAGGCCTAC
    TTAGTTCGCT CACAAAGAGC AACAGGCATC GGGAGGTTGA TGGTGAATAT TGTTGAAGGC
    ATTGAACTAA AACCTTGCAG ATCCCATGGA AAGAGTAATC CATACTGTGA GGTGACGATG
    GGCTCTCAGT GCCACATCAC CAAGACAATG CAGGACACCC TCAATCCAAA GTGGAACTCC
    AACTGCCAGT TCTTTATCAA AGACCTCGAG CAGGACGTGC TCTGCATCAC GGTGTTTGAG
    AGGGACCAGT TCTCCCCAGA TGACTTTTTG GGCAGAACAG AGATCCGTGT GGCAGACATT
    AAGAAGGATC AGGGTTCAAA GGGACCAGTT ACTAAGTGTC TCCTTCTCCA TGAAGTCCCA
    ACAGGAGAGA TAGTCGTCCG ACTAGACCTG CAATTGTTTG ATGAGCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_416715.2
    CDS231..5381
    Translation

    Target ORF information:

    RefSeq Version XM_416715.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus intersectin 1 (ITSN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_416715.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCAACACC TTTTGGGGGC AACCTGGATA TCTGGGCTAT TACTGTGGAG 
    GAGAGAGCTA AACATGATCA ACAGTTCCAC AGTCTGAAAC CAACATCTGG ATTTATCACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAG TCTGGGTTAC CTCAGCCGGT GTTAGCACAG
    ATATGGGCTT TAGCTGACAT GAACAATGAT GGAAGGATGG ATCAGCTGGA GTTTTCAATA
    GCTATGAAAC TTATCAAATT AAAACTACAA GGCTATCAAC TCCCATCTGC GCTGCCTCCT
    GTCATGAAGC AGCCACCTAT TGCTCTACCT AGTGCACCAG GGTTTGGTAT TGGGGGCATT
    GCCAGCATGC CATCTCTTAC AACTGTTGCC CCAGTGCCAA TGGCATCTAT TCCAGTAGTA
    GGAATGTCTC CGCCATTAGT ATCTTCTGTT CCTGCAGCAG CAGTGCCTCC CCTGGCTAAT
    GGAGCTCCTG CTGTTATACA GCCTCTGCCT GCTTTTGCTC ATCCTGCCAC ATTGCCAAAG
    AGTTCTTCCT TTAGTAGATC TGGTCCAGGA TCGCAGCTGA ACACAAAACT GCAAAAGGCA
    CAATCGTTTG ACGTGGCGAG TGTGCCTCCT GTGGCAGAGT GGGCAGTACC TCAGTCATCA
    AGACTGAAAT ACAGACAGTT GTTCAATAGC CACGATAAAA CAATGAGTGG ACACTTAACA
    GGTCCGCAAG CAAGAACTAT TCTTATGCAG TCAAGTTTAC CCCAGGCTCA GCTGGCTACA
    ATATGGAATC TTTCAGATAT TGATCAAGAT GGAAAGCTTA CAGCTGAAGA GTTTATTCTG
    GCTATGCACT TAATTGATGT AGCTATGTCT GGTCAGCCAC TGCCACCTGT ACTGCCTCCA
    GAGTATATTC CACCTTCATT TAGAAGAGTA CGCTCTGGTA GTGGTGTATC TGCTGTAAGT
    TCAGTATCTG TAGACCAAAG GTTACCAGAA GAACCAGCAT TAGAAGAAGA ACAGCAGCAA
    CTGGAAAAGA AATTGCCAGT TACATTTGAA GATAAAAAAC GTGAGAACTT TGAACGTGGC
    AATCTAGAAC TTGAAAAACG GAGGCAGGCT CTCCTGGAAC AGCAACGCAA AGAGCAAGAG
    CGTCTAGCAC AGCTGGAACG GGCAGAGCAA GAAAGGAAGG AACGTGAACG ACAAGAGCAA
    GAAAGGAAAA GACAGCTGGA GCTAGAGAAA CAGTTGGAGA AACAACGGGA ATTGGAACGG
    CAGAGAGAAG AGGAAAGGAG GAAAGAAATA GAAAGAAGAG AGGCTGCAAA ACGGGAACTT
    GAGAGGCAAA GGCAACTTGA ATGGGAACGT AATCGTCGGC AAGAACTGCT AAATCAAAGA
    AACAGAGAAC AAGAGGACAT CGTTGTTCTG AAGGCAAAGA AGAAGACTTT AGAATTTGAG
    CTGGAAGCTC TAAATGATAA AAAAAATCAG TTGGAAGGAA AGCTTCAGGA TATCAGATGT
    CGGCTGTCTA CCCAAAGACA AGAAATTGAA AGTACAAATA AATCTAGAGA ACTGAGAATT
    GCAGAAATCA CCCATTTGCA ACAGCAGCTA CAGGAGTCTC AGCAGATGCT TGGAAAGTTG
    ATTCCAGAAA AGCAATTACT CAATGACCAA CTAAAGCAGG TTCAACAGAA CAGTTTGCAT
    AGAGATTCTC TTCTTACTAT CAAAAGAGCC TTAGAAGCCA AGGAACTAGC CCGTCAACAG
    CTTCGAGACC AGCTAGATGA AGTAGAAAAA GAAACCAGAT CTAAACTTCA GGAAATTGAT
    ATTTTCAATA ATCAGCTGAA GGAGCTGAGA GAAATACATA ACAGACAGCA GCTTCAGAAA
    CAAAAGAACT TGGAAGCTGA GAGGCTGAAA CAGAAAGAAC AAGAAAGGAA GACAGAACTG
    GAAAAGCAAA AAGAAGCTCA AAGGCGAATC CAGGATCGGG ATAAACAACG GCTTGATCGA
    GTGCAACAAG AAGAAGAACC TCAATGGCAA AAAAAAAATC AAGAAGATGA CAAGCAAAAA
    AGGGAAGAAA TAATCAAGAA GAAGGAAAGT GAGGATAAGG GGAAACAGGA AATCCAAGAG
    AAACCGAGTA AGCTTTTCCA ACCACATCAA GAACCAGTTA AGCCAGCTGT CCAGGCTCCA
    TGGTCAAATG CAGGAAAAGC TCCACTAACA ATTTCTGCTC AGGAGGATGT AAAAATAGTG
    TATTATAGAG CACTGTATCC TTTTGAATCA AGAAGCCATG ACGAAATCAC TATTCAACCT
    GGCGACATAG TCATGGTTAA AAGGGAATGG GTGGATGAAA GTCAGACTGG AGAACCAGGG
    TGGCTTGGTG GAGAATTGAA GGGAAAAACT GGATGGTTCC CAGCAAACTA CGCAGAAAAA
    ATACCTGAAA GTGAAGTTCC AGCATCTATA AAACCAGTTG AGGCAGCTCC TGCACCTAAA
    GTTTCTGTGC ATGAAACAAC CACATCACTA GGAACTGCTG CATCTACAGA GTGCACGACA
    ACTGCTAACA ACTGGGCAGA CTTCAGTTCA ACATGGCCAG CAAACACTAA TGAGAAACCA
    GAGACAGACA ACTGGGATGC CTGGGCAGCT CAACCATCTT TGACTGTTCC CAGTGCAGGG
    CAGCTGCGGC AGCGATCAGC CTTCACCCCC GCCACTGTTA CAGGATCGTC TCCCTCTCCT
    GTATTGGGTC AGGGTGAAAA AGTGGAGGGA CTTCAAGCAC AGGCTCTGTA TCCTTGGAGA
    GCAAAAAAAG ACAACCACCT AAATTTCAAC AAAAATGATG TCATCACAGT TTTAGAGCAG
    CAAGATATGT GGTGGTTTGG AGAAGTTCAA GGACAAAAGG GGTGGTTTCC TAAGTCATAT
    GTGAAGCTTA TTTCAGGCCC CATTAGGAAG TCAACAAGCA TGGATTCTGG TTCTTCAGAA
    AGTCCTGCTA GTCTGAAAAG AGTAGCATCA CCAGCAGCTA AAGCAACTAT GTCAGGTGAA
    GAATATGTTG CTATGTATAC TTATGAGAGT TCTGAACAAG GAGACCTAAC CTTTCAGCAG
    GGTGATATGA TTCTTGTGAC AAAGAAAGAT GGCGACTGGT GGACAGGAAC ACTGGGTGAT
    AAAACAGGAG TTTTCCCATC AAATTATGTG AGACTCAAAG ATTCTGAGGC TTCTGGAGCA
    GCTGGAAAAA CAGGAAGCTT AGGGAAGAAA CCTGAAATTG CCCAAGTTAT TGCCTCTTAC
    ACAGCAACGG GTCCAGAACA ACTCACCCTT GCTCCTGGTC AGTTGATTCT TATCAGAAAG
    AAGAACCCAG GTGGCTGGTG GGAAGGAGAG CTCCAAGCAC GAGGGAAAAA ACGACAAATA
    GGATGGTTTC CTGCTAATTA TGTGAAACTT TTGAGCCCTG GTACCAGTAA AACTACACCA
    ACCGAGCTAC CTAAATCAAC AGCATTACCT TCAGTGTGCC AAGTAATTGG CATGTATGAC
    TACACTGCAC AGAATGATGA TGAGCTAGCA TTCAATAAAG GCCAGATTAT AAACGTCCTT
    AACAAGGAAG ACCCAGACTG GTGGAAAGGG GAGGTGAATG GACAAGTGGG TCTCTTCCCA
    TCCAACTATG TGAAGCTGAC AACAGATATG GACCCAAGCC AGCAATGGTG TGCAGACTTG
    CATCTTCTGG ATATGCTGAC ACCTACTGAA CGAAAAAGGC AGGGATACAT CCATGAGCTC
    ATTGTCACAG AAGAGAACTA CGTAAATGAT CTGCAGTTGG TCACAGAGAT CTTCCAGAAA
    CCACTAATGG AATCTGAGCT GCTAACAGAA AAGGAAGTTG CTATGATTTT TGTTAATTGG
    AAGGAGCTGA TTATGTGCAA TATAAAACTA CTGAAGGCCT TGCGTGTGCG TAAGAAGATG
    TCTGGGGAGA AGATGCCAGT GAAAATGATT GGTGACATCC TGACAGCTCA GCTGCCACAC
    ATGCAGCCAT ACATTCGGTT CTGTAGCTGC CAGCTCAATG GGGCAGCACT CATTCAGCAG
    AAAACAGATG AAGTCCCCGA ATTCAAGGAG TTTGTAAAAA GGTTAGCAAT GGATCCTCGC
    TGTAAAGGAA TGCCACTATC AAGTTTTCTA CTAAAGCCTA TGCAACGGGT AACAAGATAC
    CCACTAATAA TTAAAAATAT AATAGAAAAC ACTCCTGAAA ACCATCCTGA CCACAGTCAC
    TTGAAGCATG CTCTTGAAAA AGCAGAAGAA TTATGTTCTC AAGTAAATGA AGGAGTGCGA
    GAGAAAGAAA ATTCAGATAG GCTGGAGTGG ATCCAGGCTC ATGTTCAGTG CGAAGGCCTC
    TCTGAGCAAC TCGTATTTAA TTCTGTTACA AACTGCTTGG GACCACGCAA GTTTCTGCAC
    AGTGGGAAGC TCTATAAAGC CAAGAGTAAC AAGGAACTGT ATGGCTTTCT GTTCAACGAT
    TTCCTCCTCT TGACTCAGAT CATAAAACCT CTTGGCTCTT CTGGCACAGA CAAGGTCTTC
    AGCCCCAAGT CTAATCTGCA GTACAAAATG TACAAAACTC CCATTTTTCT AAATGAAGTA
    CTAGTAAAAT TACCCACAGA CCCTTCTGGA GATGAGCCCA TTTTCCATAT CTCCCACATT
    GACAGAGTCT ACACACTCCG GGCTGAAAGC ATTAATGAAA GGACTGCGTG GGTTCAGAAA
    ATTAAAGCTG CTTCGGAACT TTACATAGAG ACTGAAAAGA AGAAGAGGGA AAAGGCCTAC
    TTAGTTCGCT CACAAAGAGC AACAGGCATC GGGAGGTTGA TGGTGAATAT TGTTGAAGGC
    ATTGAACTAA AACCTTGCAG ATCCCATGGA AAGAGTAATC CATACTGTGA GGTGACGATG
    GGCTCTCAGT GCCACATCAC CAAGACAATG CAGGACACCC TCAATCCAAA GTGGAACTCC
    AACTGCCAGT TCTTTATCAA AGACCTCGAG CAGGACGTGC TCTGCATCAC GGTGTTTGAG
    AGGGACCAGT TCTCCCCAGA TGACTTTTTG GGCAGAACAG AGATCCGTGT GGCAGACATT
    AAGAAGGATC AGGGTTCAAA GGGACCAGTT ACTAAGTGTC TCCTTCTCCA TGAAGTCCCA
    ACAGGAGAGA TAGTCGTCCG ACTAGACCTG CAATTGTTTG ATGAGCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.