GAAGSD cDNA ORF clone, Gallus gallus(chicken)

The following GAAGSD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAAGSD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa42300 XM_015294335.2
Latest version!
Gallus gallus glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (GAAGSD), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa42300 XM_025155387.1
Latest version!
Gallus gallus glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (GAAGSD), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa42301 XM_015294337.2
Latest version!
Gallus gallus glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (GAAGSD), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa42301 XM_015294336.2
Latest version!
Gallus gallus glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (GAAGSD), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
View Old Accession Versions >>
OGa42301 XM_015294336.1 Gallus gallus glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (GAA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa42300 XM_015294335.1 Gallus gallus glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (GAA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa42301 XM_015294337.1 Gallus gallus glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (GAA), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa42300
    Clone ID Related Accession (Same CDS sequence) XM_015294335.1 , XM_025155387.1 , XM_015294335.2
    Accession Version XM_015294335.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product lysosomal alpha-glucosidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACAATGA GGTCGTACCA GAAGCTGAGG ACGGCGGTGC CGCAGCTGGT GTTGAGCCAG 
    GAGGAGGAGG AGGAGAGTGC GGCCACCCCA ACACCTGCTG GCCGCAGCAA GCTGGCCCCG
    TGGTGGGTGG GCAGCGGGCT GCTGATCACC GCCGTGCTGC TGAGCACCAT CACTGTCTGG
    GTGCTGCGAC AGGTGTCCAG GGGCTGGCAC GGCCCCGCAC CACCTTCCCA ATGTCACCTG
    GTCCCTGAGA GCCACCGCTA TGACTGCTAC CCCGAAAGGA GCGTGGTGGT GACCCAGGAG
    CTGTGCGAGA GCCGGGGGTG CTGCTTCATC GAGACCCCCC CTGCAGTGGG GGGCAAGAGG
    GGGGTGCCCT GGTGCTTCTA CCCACCCAGC TTCCCCAGCT ACGTGGTGCA GAGCCTCAAC
    CAGACGGCGC TGGGCATGAC GGGGCTGCTG GTGCGCAGGG AGAAGGCCTA TTACCCCAAG
    GACATCCAGG TGCTGAGGAT GGACGTGGAG TTCCAGACGA ACACACGGCT GCGCATCAAG
    ATAACCGATG CAGCCAAGCC CCGGTATGAG GTGCCCCTGG AAGTTCCCCG AGTGATGAAG
    AGAGCAGAAA ACCCCATCTA CAGCCTGGAG TTCTCCCAGG ACCCCTTTGG GGTGCTGCTG
    AGGCGCCAAG GGACAGGGAC AGTGCTGCTC AACACCACCG TGGCTCCCCT GATCTTCGCC
    GATCAGTTCC TGCAGATCTC CACCACGCTG CCCTCCAGGT TCCTGTATGG GCTGGGGGAG
    CACCGCAGCA CTCTGCTGCA CAGCCTCGAC TGGAACACCC TCACCCTCTG GGCCCGTGAT
    GTGGCCCCCA CGGAATCCTT CAACCTGTAC GGCGCTCACC CCTTCTACCT GCTGATGGAG
    GAGGGCGGCG ATGCCCACGG GGTTTTCCTC CTCAACAGCA ACGCCATGGA GGTGGCCCTG
    CAGCCTGCCC CGGGCCTGAC TTGGAGGACC ATTGGAGGTG TGCTGGACTT CTACATCTTC
    CTGGGGCCTG ACCCCAACAT GGTCATCCAG CAGTACCAGG AGGTGATAGG TTTCCCAGCC
    ATGCCTCCAC TCTGGGCGCT CGGCTTCCAC CTCTGCCGCT GGGGCTACGG CTCCAGCAAC
    GAGACCTGGC AGACTGCAAG GGCCATGAGG AACTTCCAGA TCCCACAGGA TGCGCAATGG
    AACGACATTG ATTACATGGA CGGGTACCGG GACTTCACCT TCGATCCCCA GAAATTCGCC
    TCCCTCCCCT CACTGGTGGA AGATCTCCAC AAGCACGGGC AGCACTACGT GATGATCTTG
    GACCCCGGCA TCAGCAGCAC CAGCCCCCAC GGCTCCTACT GGCCTTTCGA TGAAGGCTTG
    AGACGTGCTT TGTTCCTGAA CACCACCCAG GGGCAGCCAC TGATCGGGCA GGTCTGGCCT
    GGTTATACTG CCTTCCCCGA CTTCTCCAAC AGAGACACAC ATCAGTGGTG GCTGGAGAAC
    CTGCAGCGCT TCCACACCCG TGTGCCCTTC GATGGCCTCT GGATCGACAT GAACGAGCCA
    TCCAACTTCA TGGATGGGTC TGAAGAGGGC TGCCCACCCG GAGAGCTCGA CAGCCCCCCG
    TACACGCCGG CTGTGCTGGG CAATTCCCTG TCCGCCAAGA CGGTGTGTGC CTCAGCGAAG
    CAGAACGCCT CGGTGCACTA CAACCTGCAC AACCTCTACG GGCTGAAGGA AGCCGAAGCG
    ACGGCGAGCG CCTTGATCCA CATCCGAGGA AAGCGTCCCT TTGTCATCTC CCGCTCTACC
    TTCCCCAGCC AGGGCCGGTA CTCGGGTCAC TGGCTGGGTG ACAACCGGAG CCAGTGGAAG
    GACATGTATT ACTCCATCCC AGGGATGCTG AGCTTCAGCC TGTTTGGGAT CCCACTGGTG
    GGGGCCGACA TCTGCGGCTT CTCCGGCAGC ACCTCGGAGG AGCTGTGCAC CCGCTGGATG
    CAGCTCGGGG CCTTCTACCC CTTCTCCCGC AACCACAACA CCCAGAACGA GAAGGCCCAG
    GACCCCACAG CTTTCAGCCC CTCGGCGAGG ACAGCCATGA AGGACGTACT GCTCACCCGC
    TACTCCCTCC TGCCTTTCCT CTACACACTT TTCCACCGTG CCCACCTGCA AGGGGAAACC
    GTTGCCCGAC CTTTGTTCTT TGAGTTCCCC TGGGATGTGG CCACGTATGG GCTGGACAGG
    CAGTTCCTGT GGGGCCAGAG CCTGCTGGTG ACACCGGTGC TGGAGCCCGG AGCAGACTCG
    GTGTTGGGCT ATTTCCCCCG AGGCGTGTGG TACGACTTCT ACACGGGCTC CTCTGTGAAC
    AGCAGTGGGG AGATGTTGAA GCTGTCAGCC CCCCTGGACC ACCTCAACCT GCATCTCCGG
    GAGGGTTCTA TCCTGCCCAC ACAGAAACCC GGGACCACCA GCAAGGCCAC CCGAGGGAAC
    CCGCTGCGCC TCATTGTGGC CCTGTCCCCA CGTGCCACTG CCTGGGGGGA CCTCTTCTGG
    GATGACGGCG AGAGCCTGGA CACCTTCGAG CGGGGCAACT ACTCCTACCT GGTGTTCAAT
    GCCACAGAGA ACATCTTCAC CTCCAACGTC CTCCATGCCA GCACCGAGGC CACCTATGTC
    ACCATCGACA CGGTGAGCTT CTACGGAATG CAGGAGCCGC CCAGCAAGGT CCTCCTGGAT
    GGGCAGGAGA AGCCCTTCTC CTACCTGGAC AACCAGGTCC TCACGGTCAG CGGCCTCGGC
    CTCAGGCTCA GCCAGGGCTT CTCCCTGCAG TGGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149821.1
    CDS1614..4412
    Translation

    Target ORF information:

    RefSeq Version XM_015294335.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (GAA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294335.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACAATGA GGTCGTACCA GAAGCTGAGG ACGGCGGTGC CGCAGCTGGT GTTGAGCCAG 
    GAGGAGGAGG AGGAGAGTGC GGCCACCCCA ACACCTGCTG GCCGCAGCAA GCTGGCCCCG
    TGGTGGGTGG GCAGCGGGCT GCTGATCACC GCCGTGCTGC TGAGCACCAT CACTGTCTGG
    GTGCTGCGAC AGGTGTCCAG GGGCTGGCAC GGCCCCGCAC CACCTTCCCA ATGTCACCTG
    GTCCCTGAGA GCCACCGCTA TGACTGCTAC CCCGAAAGGA GCGTGGTGGT GACCCAGGAG
    CTGTGCGAGA GCCGGGGGTG CTGCTTCATC GAGACCCCCC CTGCAGTGGG GGGCAAGAGG
    GGGGTGCCCT GGTGCTTCTA CCCACCCAGC TTCCCCAGCT ACGTGGTGCA GAGCCTCAAC
    CAGACGGCGC TGGGCATGAC GGGGCTGCTG GTGCGCAGGG AGAAGGCCTA TTACCCCAAG
    GACATCCAGG TGCTGAGGAT GGACGTGGAG TTCCAGACGA ACACACGGCT GCGCATCAAG
    ATAACCGATG CAGCCAAGCC CCGGTATGAG GTGCCCCTGG AAGTTCCCCG AGTGATGAAG
    AGAGCAGAAA ACCCCATCTA CAGCCTGGAG TTCTCCCAGG ACCCCTTTGG GGTGCTGCTG
    AGGCGCCAAG GGACAGGGAC AGTGCTGCTC AACACCACCG TGGCTCCCCT GATCTTCGCC
    GATCAGTTCC TGCAGATCTC CACCACGCTG CCCTCCAGGT TCCTGTATGG GCTGGGGGAG
    CACCGCAGCA CTCTGCTGCA CAGCCTCGAC TGGAACACCC TCACCCTCTG GGCCCGTGAT
    GTGGCCCCCA CGGAATCCTT CAACCTGTAC GGCGCTCACC CCTTCTACCT GCTGATGGAG
    GAGGGCGGCG ATGCCCACGG GGTTTTCCTC CTCAACAGCA ACGCCATGGA GGTGGCCCTG
    CAGCCTGCCC CGGGCCTGAC TTGGAGGACC ATTGGAGGTG TGCTGGACTT CTACATCTTC
    CTGGGGCCTG ACCCCAACAT GGTCATCCAG CAGTACCAGG AGGTGATAGG TTTCCCAGCC
    ATGCCTCCAC TCTGGGCGCT CGGCTTCCAC CTCTGCCGCT GGGGCTACGG CTCCAGCAAC
    GAGACCTGGC AGACTGCAAG GGCCATGAGG AACTTCCAGA TCCCACAGGA TGCGCAATGG
    AACGACATTG ATTACATGGA CGGGTACCGG GACTTCACCT TCGATCCCCA GAAATTCGCC
    TCCCTCCCCT CACTGGTGGA AGATCTCCAC AAGCACGGGC AGCACTACGT GATGATCTTG
    GACCCCGGCA TCAGCAGCAC CAGCCCCCAC GGCTCCTACT GGCCTTTCGA TGAAGGCTTG
    AGACGTGCTT TGTTCCTGAA CACCACCCAG GGGCAGCCAC TGATCGGGCA GGTCTGGCCT
    GGTTATACTG CCTTCCCCGA CTTCTCCAAC AGAGACACAC ATCAGTGGTG GCTGGAGAAC
    CTGCAGCGCT TCCACACCCG TGTGCCCTTC GATGGCCTCT GGATCGACAT GAACGAGCCA
    TCCAACTTCA TGGATGGGTC TGAAGAGGGC TGCCCACCCG GAGAGCTCGA CAGCCCCCCG
    TACACGCCGG CTGTGCTGGG CAATTCCCTG TCCGCCAAGA CGGTGTGTGC CTCAGCGAAG
    CAGAACGCCT CGGTGCACTA CAACCTGCAC AACCTCTACG GGCTGAAGGA AGCCGAAGCG
    ACGGCGAGCG CCTTGATCCA CATCCGAGGA AAGCGTCCCT TTGTCATCTC CCGCTCTACC
    TTCCCCAGCC AGGGCCGGTA CTCGGGTCAC TGGCTGGGTG ACAACCGGAG CCAGTGGAAG
    GACATGTATT ACTCCATCCC AGGGATGCTG AGCTTCAGCC TGTTTGGGAT CCCACTGGTG
    GGGGCCGACA TCTGCGGCTT CTCCGGCAGC ACCTCGGAGG AGCTGTGCAC CCGCTGGATG
    CAGCTCGGGG CCTTCTACCC CTTCTCCCGC AACCACAACA CCCAGAACGA GAAGGCCCAG
    GACCCCACAG CTTTCAGCCC CTCGGCGAGG ACAGCCATGA AGGACGTACT GCTCACCCGC
    TACTCCCTCC TGCCTTTCCT CTACACACTT TTCCACCGTG CCCACCTGCA AGGGGAAACC
    GTTGCCCGAC CTTTGTTCTT TGAGTTCCCC TGGGATGTGG CCACGTATGG GCTGGACAGG
    CAGTTCCTGT GGGGCCAGAG CCTGCTGGTG ACACCGGTGC TGGAGCCCGG AGCAGACTCG
    GTGTTGGGCT ATTTCCCCCG AGGCGTGTGG TACGACTTCT ACACGGGCTC CTCTGTGAAC
    AGCAGTGGGG AGATGTTGAA GCTGTCAGCC CCCCTGGACC ACCTCAACCT GCATCTCCGG
    GAGGGTTCTA TCCTGCCCAC ACAGAAACCC GGGACCACCA GCAAGGCCAC CCGAGGGAAC
    CCGCTGCGCC TCATTGTGGC CCTGTCCCCA CGTGCCACTG CCTGGGGGGA CCTCTTCTGG
    GATGACGGCG AGAGCCTGGA CACCTTCGAG CGGGGCAACT ACTCCTACCT GGTGTTCAAT
    GCCACAGAGA ACATCTTCAC CTCCAACGTC CTCCATGCCA GCACCGAGGC CACCTATGTC
    ACCATCGACA CGGTGAGCTT CTACGGAATG CAGGAGCCGC CCAGCAAGGT CCTCCTGGAT
    GGGCAGGAGA AGCCCTTCTC CTACCTGGAC AACCAGGTCC TCACGGTCAG CGGCCTCGGC
    CTCAGGCTCA GCCAGGGCTT CTCCCTGCAG TGGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42300
    Clone ID Related Accession (Same CDS sequence) XM_015294335.1 , XM_025155387.1 , XM_015294335.2
    Accession Version XM_025155387.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product lysosomal alpha-glucosidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACAATGA GGTCGTACCA GAAGCTGAGG ACGGCGGTGC CGCAGCTGGT GTTGAGCCAG 
    GAGGAGGAGG AGGAGAGTGC GGCCACCCCA ACACCTGCTG GCCGCAGCAA GCTGGCCCCG
    TGGTGGGTGG GCAGCGGGCT GCTGATCACC GCCGTGCTGC TGAGCACCAT CACTGTCTGG
    GTGCTGCGAC AGGTGTCCAG GGGCTGGCAC GGCCCCGCAC CACCTTCCCA ATGTCACCTG
    GTCCCTGAGA GCCACCGCTA TGACTGCTAC CCCGAAAGGA GCGTGGTGGT GACCCAGGAG
    CTGTGCGAGA GCCGGGGGTG CTGCTTCATC GAGACCCCCC CTGCAGTGGG GGGCAAGAGG
    GGGGTGCCCT GGTGCTTCTA CCCACCCAGC TTCCCCAGCT ACGTGGTGCA GAGCCTCAAC
    CAGACGGCGC TGGGCATGAC GGGGCTGCTG GTGCGCAGGG AGAAGGCCTA TTACCCCAAG
    GACATCCAGG TGCTGAGGAT GGACGTGGAG TTCCAGACGA ACACACGGCT GCGCATCAAG
    ATAACCGATG CAGCCAAGCC CCGGTATGAG GTGCCCCTGG AAGTTCCCCG AGTGATGAAG
    AGAGCAGAAA ACCCCATCTA CAGCCTGGAG TTCTCCCAGG ACCCCTTTGG GGTGCTGCTG
    AGGCGCCAAG GGACAGGGAC AGTGCTGCTC AACACCACCG TGGCTCCCCT GATCTTCGCC
    GATCAGTTCC TGCAGATCTC CACCACGCTG CCCTCCAGGT TCCTGTATGG GCTGGGGGAG
    CACCGCAGCA CTCTGCTGCA CAGCCTCGAC TGGAACACCC TCACCCTCTG GGCCCGTGAT
    GTGGCCCCCA CGGAATCCTT CAACCTGTAC GGCGCTCACC CCTTCTACCT GCTGATGGAG
    GAGGGCGGCG ATGCCCACGG GGTTTTCCTC CTCAACAGCA ACGCCATGGA GGTGGCCCTG
    CAGCCTGCCC CGGGCCTGAC TTGGAGGACC ATTGGAGGTG TGCTGGACTT CTACATCTTC
    CTGGGGCCTG ACCCCAACAT GGTCATCCAG CAGTACCAGG AGGTGATAGG TTTCCCAGCC
    ATGCCTCCAC TCTGGGCGCT CGGCTTCCAC CTCTGCCGCT GGGGCTACGG CTCCAGCAAC
    GAGACCTGGC AGACTGCAAG GGCCATGAGG AACTTCCAGA TCCCACAGGA TGCGCAATGG
    AACGACATTG ATTACATGGA CGGGTACCGG GACTTCACCT TCGATCCCCA GAAATTCGCC
    TCCCTCCCCT CACTGGTGGA AGATCTCCAC AAGCACGGGC AGCACTACGT GATGATCTTG
    GACCCCGGCA TCAGCAGCAC CAGCCCCCAC GGCTCCTACT GGCCTTTCGA TGAAGGCTTG
    AGACGTGCTT TGTTCCTGAA CACCACCCAG GGGCAGCCAC TGATCGGGCA GGTCTGGCCT
    GGTTATACTG CCTTCCCCGA CTTCTCCAAC AGAGACACAC ATCAGTGGTG GCTGGAGAAC
    CTGCAGCGCT TCCACACCCG TGTGCCCTTC GATGGCCTCT GGATCGACAT GAACGAGCCA
    TCCAACTTCA TGGATGGGTC TGAAGAGGGC TGCCCACCCG GAGAGCTCGA CAGCCCCCCG
    TACACGCCGG CTGTGCTGGG CAATTCCCTG TCCGCCAAGA CGGTGTGTGC CTCAGCGAAG
    CAGAACGCCT CGGTGCACTA CAACCTGCAC AACCTCTACG GGCTGAAGGA AGCCGAAGCG
    ACGGCGAGCG CCTTGATCCA CATCCGAGGA AAGCGTCCCT TTGTCATCTC CCGCTCTACC
    TTCCCCAGCC AGGGCCGGTA CTCGGGTCAC TGGCTGGGTG ACAACCGGAG CCAGTGGAAG
    GACATGTATT ACTCCATCCC AGGGATGCTG AGCTTCAGCC TGTTTGGGAT CCCACTGGTG
    GGGGCCGACA TCTGCGGCTT CTCCGGCAGC ACCTCGGAGG AGCTGTGCAC CCGCTGGATG
    CAGCTCGGGG CCTTCTACCC CTTCTCCCGC AACCACAACA CCCAGAACGA GAAGGCCCAG
    GACCCCACAG CTTTCAGCCC CTCGGCGAGG ACAGCCATGA AGGACGTACT GCTCACCCGC
    TACTCCCTCC TGCCTTTCCT CTACACACTT TTCCACCGTG CCCACCTGCA AGGGGAAACC
    GTTGCCCGAC CTTTGTTCTT TGAGTTCCCC TGGGATGTGG CCACGTATGG GCTGGACAGG
    CAGTTCCTGT GGGGCCAGAG CCTGCTGGTG ACACCGGTGC TGGAGCCCGG AGCAGACTCG
    GTGTTGGGCT ATTTCCCCCG AGGCGTGTGG TACGACTTCT ACACGGGCTC CTCTGTGAAC
    AGCAGTGGGG AGATGTTGAA GCTGTCAGCC CCCCTGGACC ACCTCAACCT GCATCTCCGG
    GAGGGTTCTA TCCTGCCCAC ACAGAAACCC GGGACCACCA GCAAGGCCAC CCGAGGGAAC
    CCGCTGCGCC TCATTGTGGC CCTGTCCCCA CGTGCCACTG CCTGGGGGGA CCTCTTCTGG
    GATGACGGCG AGAGCCTGGA CACCTTCGAG CGGGGCAACT ACTCCTACCT GGTGTTCAAT
    GCCACAGAGA ACATCTTCAC CTCCAACGTC CTCCATGCCA GCACCGAGGC CACCTATGTC
    ACCATCGACA CGGTGAGCTT CTACGGAATG CAGGAGCCGC CCAGCAAGGT CCTCCTGGAT
    GGGCAGGAGA AGCCCTTCTC CTACCTGGAC AACCAGGTCC TCACGGTCAG CGGCCTCGGC
    CTCAGGCTCA GCCAGGGCTT CTCCCTGCAG TGGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025011155.1
    CDS2934..5732
    Translation

    Target ORF information:

    RefSeq Version XM_025155387.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (GAAGSD), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025155387.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACAATGA GGTCGTACCA GAAGCTGAGG ACGGCGGTGC CGCAGCTGGT GTTGAGCCAG 
    GAGGAGGAGG AGGAGAGTGC GGCCACCCCA ACACCTGCTG GCCGCAGCAA GCTGGCCCCG
    TGGTGGGTGG GCAGCGGGCT GCTGATCACC GCCGTGCTGC TGAGCACCAT CACTGTCTGG
    GTGCTGCGAC AGGTGTCCAG GGGCTGGCAC GGCCCCGCAC CACCTTCCCA ATGTCACCTG
    GTCCCTGAGA GCCACCGCTA TGACTGCTAC CCCGAAAGGA GCGTGGTGGT GACCCAGGAG
    CTGTGCGAGA GCCGGGGGTG CTGCTTCATC GAGACCCCCC CTGCAGTGGG GGGCAAGAGG
    GGGGTGCCCT GGTGCTTCTA CCCACCCAGC TTCCCCAGCT ACGTGGTGCA GAGCCTCAAC
    CAGACGGCGC TGGGCATGAC GGGGCTGCTG GTGCGCAGGG AGAAGGCCTA TTACCCCAAG
    GACATCCAGG TGCTGAGGAT GGACGTGGAG TTCCAGACGA ACACACGGCT GCGCATCAAG
    ATAACCGATG CAGCCAAGCC CCGGTATGAG GTGCCCCTGG AAGTTCCCCG AGTGATGAAG
    AGAGCAGAAA ACCCCATCTA CAGCCTGGAG TTCTCCCAGG ACCCCTTTGG GGTGCTGCTG
    AGGCGCCAAG GGACAGGGAC AGTGCTGCTC AACACCACCG TGGCTCCCCT GATCTTCGCC
    GATCAGTTCC TGCAGATCTC CACCACGCTG CCCTCCAGGT TCCTGTATGG GCTGGGGGAG
    CACCGCAGCA CTCTGCTGCA CAGCCTCGAC TGGAACACCC TCACCCTCTG GGCCCGTGAT
    GTGGCCCCCA CGGAATCCTT CAACCTGTAC GGCGCTCACC CCTTCTACCT GCTGATGGAG
    GAGGGCGGCG ATGCCCACGG GGTTTTCCTC CTCAACAGCA ACGCCATGGA GGTGGCCCTG
    CAGCCTGCCC CGGGCCTGAC TTGGAGGACC ATTGGAGGTG TGCTGGACTT CTACATCTTC
    CTGGGGCCTG ACCCCAACAT GGTCATCCAG CAGTACCAGG AGGTGATAGG TTTCCCAGCC
    ATGCCTCCAC TCTGGGCGCT CGGCTTCCAC CTCTGCCGCT GGGGCTACGG CTCCAGCAAC
    GAGACCTGGC AGACTGCAAG GGCCATGAGG AACTTCCAGA TCCCACAGGA TGCGCAATGG
    AACGACATTG ATTACATGGA CGGGTACCGG GACTTCACCT TCGATCCCCA GAAATTCGCC
    TCCCTCCCCT CACTGGTGGA AGATCTCCAC AAGCACGGGC AGCACTACGT GATGATCTTG
    GACCCCGGCA TCAGCAGCAC CAGCCCCCAC GGCTCCTACT GGCCTTTCGA TGAAGGCTTG
    AGACGTGCTT TGTTCCTGAA CACCACCCAG GGGCAGCCAC TGATCGGGCA GGTCTGGCCT
    GGTTATACTG CCTTCCCCGA CTTCTCCAAC AGAGACACAC ATCAGTGGTG GCTGGAGAAC
    CTGCAGCGCT TCCACACCCG TGTGCCCTTC GATGGCCTCT GGATCGACAT GAACGAGCCA
    TCCAACTTCA TGGATGGGTC TGAAGAGGGC TGCCCACCCG GAGAGCTCGA CAGCCCCCCG
    TACACGCCGG CTGTGCTGGG CAATTCCCTG TCCGCCAAGA CGGTGTGTGC CTCAGCGAAG
    CAGAACGCCT CGGTGCACTA CAACCTGCAC AACCTCTACG GGCTGAAGGA AGCCGAAGCG
    ACGGCGAGCG CCTTGATCCA CATCCGAGGA AAGCGTCCCT TTGTCATCTC CCGCTCTACC
    TTCCCCAGCC AGGGCCGGTA CTCGGGTCAC TGGCTGGGTG ACAACCGGAG CCAGTGGAAG
    GACATGTATT ACTCCATCCC AGGGATGCTG AGCTTCAGCC TGTTTGGGAT CCCACTGGTG
    GGGGCCGACA TCTGCGGCTT CTCCGGCAGC ACCTCGGAGG AGCTGTGCAC CCGCTGGATG
    CAGCTCGGGG CCTTCTACCC CTTCTCCCGC AACCACAACA CCCAGAACGA GAAGGCCCAG
    GACCCCACAG CTTTCAGCCC CTCGGCGAGG ACAGCCATGA AGGACGTACT GCTCACCCGC
    TACTCCCTCC TGCCTTTCCT CTACACACTT TTCCACCGTG CCCACCTGCA AGGGGAAACC
    GTTGCCCGAC CTTTGTTCTT TGAGTTCCCC TGGGATGTGG CCACGTATGG GCTGGACAGG
    CAGTTCCTGT GGGGCCAGAG CCTGCTGGTG ACACCGGTGC TGGAGCCCGG AGCAGACTCG
    GTGTTGGGCT ATTTCCCCCG AGGCGTGTGG TACGACTTCT ACACGGGCTC CTCTGTGAAC
    AGCAGTGGGG AGATGTTGAA GCTGTCAGCC CCCCTGGACC ACCTCAACCT GCATCTCCGG
    GAGGGTTCTA TCCTGCCCAC ACAGAAACCC GGGACCACCA GCAAGGCCAC CCGAGGGAAC
    CCGCTGCGCC TCATTGTGGC CCTGTCCCCA CGTGCCACTG CCTGGGGGGA CCTCTTCTGG
    GATGACGGCG AGAGCCTGGA CACCTTCGAG CGGGGCAACT ACTCCTACCT GGTGTTCAAT
    GCCACAGAGA ACATCTTCAC CTCCAACGTC CTCCATGCCA GCACCGAGGC CACCTATGTC
    ACCATCGACA CGGTGAGCTT CTACGGAATG CAGGAGCCGC CCAGCAAGGT CCTCCTGGAT
    GGGCAGGAGA AGCCCTTCTC CTACCTGGAC AACCAGGTCC TCACGGTCAG CGGCCTCGGC
    CTCAGGCTCA GCCAGGGCTT CTCCCTGCAG TGGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42300
    Clone ID Related Accession (Same CDS sequence) XM_015294335.1 , XM_025155387.1 , XM_015294335.2
    Accession Version XM_015294335.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product lysosomal alpha-glucosidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294335.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACAATGA GGTCGTACCA GAAGCTGAGG ACGGCGGTGC CGCAGCTGGT GTTGAGCCAG 
    GAGGAGGAGG AGGAGAGTGC GGCCACCCCA ACACCTGCTG GCCGCAGCAA GCTGGCCCCG
    TGGTGGGTGG GCAGCGGGCT GCTGATCACC GCCGTGCTGC TGAGCACCAT CACTGTCTGG
    GTGCTGCGAC AGGTGTCCAG GGGCTGGCAC GGCCCCGCAC CACCTTCCCA ATGTCACCTG
    GTCCCTGAGA GCCACCGCTA TGACTGCTAC CCCGAAAGGA GCGTGGTGGT GACCCAGGAG
    CTGTGCGAGA GCCGGGGGTG CTGCTTCATC GAGACCCCCC CTGCAGTGGG GGGCAAGAGG
    GGGGTGCCCT GGTGCTTCTA CCCACCCAGC TTCCCCAGCT ACGTGGTGCA GAGCCTCAAC
    CAGACGGCGC TGGGCATGAC GGGGCTGCTG GTGCGCAGGG AGAAGGCCTA TTACCCCAAG
    GACATCCAGG TGCTGAGGAT GGACGTGGAG TTCCAGACGA ACACACGGCT GCGCATCAAG
    ATAACCGATG CAGCCAAGCC CCGGTATGAG GTGCCCCTGG AAGTTCCCCG AGTGATGAAG
    AGAGCAGAAA ACCCCATCTA CAGCCTGGAG TTCTCCCAGG ACCCCTTTGG GGTGCTGCTG
    AGGCGCCAAG GGACAGGGAC AGTGCTGCTC AACACCACCG TGGCTCCCCT GATCTTCGCC
    GATCAGTTCC TGCAGATCTC CACCACGCTG CCCTCCAGGT TCCTGTATGG GCTGGGGGAG
    CACCGCAGCA CTCTGCTGCA CAGCCTCGAC TGGAACACCC TCACCCTCTG GGCCCGTGAT
    GTGGCCCCCA CGGAATCCTT CAACCTGTAC GGCGCTCACC CCTTCTACCT GCTGATGGAG
    GAGGGCGGCG ATGCCCACGG GGTTTTCCTC CTCAACAGCA ACGCCATGGA GGTGGCCCTG
    CAGCCTGCCC CGGGCCTGAC TTGGAGGACC ATTGGAGGTG TGCTGGACTT CTACATCTTC
    CTGGGGCCTG ACCCCAACAT GGTCATCCAG CAGTACCAGG AGGTGATAGG TTTCCCAGCC
    ATGCCTCCAC TCTGGGCGCT CGGCTTCCAC CTCTGCCGCT GGGGCTACGG CTCCAGCAAC
    GAGACCTGGC AGACTGCAAG GGCCATGAGG AACTTCCAGA TCCCACAGGA TGCGCAATGG
    AACGACATTG ATTACATGGA CGGGTACCGG GACTTCACCT TCGATCCCCA GAAATTCGCC
    TCCCTCCCCT CACTGGTGGA AGATCTCCAC AAGCACGGGC AGCACTACGT GATGATCTTG
    GACCCCGGCA TCAGCAGCAC CAGCCCCCAC GGCTCCTACT GGCCTTTCGA TGAAGGCTTG
    AGACGTGCTT TGTTCCTGAA CACCACCCAG GGGCAGCCAC TGATCGGGCA GGTCTGGCCT
    GGTTATACTG CCTTCCCCGA CTTCTCCAAC AGAGACACAC ATCAGTGGTG GCTGGAGAAC
    CTGCAGCGCT TCCACACCCG TGTGCCCTTC GATGGCCTCT GGATCGACAT GAACGAGCCA
    TCCAACTTCA TGGATGGGTC TGAAGAGGGC TGCCCACCCG GAGAGCTCGA CAGCCCCCCG
    TACACGCCGG CTGTGCTGGG CAATTCCCTG TCCGCCAAGA CGGTGTGTGC CTCAGCGAAG
    CAGAACGCCT CGGTGCACTA CAACCTGCAC AACCTCTACG GGCTGAAGGA AGCCGAAGCG
    ACGGCGAGCG CCTTGATCCA CATCCGAGGA AAGCGTCCCT TTGTCATCTC CCGCTCTACC
    TTCCCCAGCC AGGGCCGGTA CTCGGGTCAC TGGCTGGGTG ACAACCGGAG CCAGTGGAAG
    GACATGTATT ACTCCATCCC AGGGATGCTG AGCTTCAGCC TGTTTGGGAT CCCACTGGTG
    GGGGCCGACA TCTGCGGCTT CTCCGGCAGC ACCTCGGAGG AGCTGTGCAC CCGCTGGATG
    CAGCTCGGGG CCTTCTACCC CTTCTCCCGC AACCACAACA CCCAGAACGA GAAGGCCCAG
    GACCCCACAG CTTTCAGCCC CTCGGCGAGG ACAGCCATGA AGGACGTACT GCTCACCCGC
    TACTCCCTCC TGCCTTTCCT CTACACACTT TTCCACCGTG CCCACCTGCA AGGGGAAACC
    GTTGCCCGAC CTTTGTTCTT TGAGTTCCCC TGGGATGTGG CCACGTATGG GCTGGACAGG
    CAGTTCCTGT GGGGCCAGAG CCTGCTGGTG ACACCGGTGC TGGAGCCCGG AGCAGACTCG
    GTGTTGGGCT ATTTCCCCCG AGGCGTGTGG TACGACTTCT ACACGGGCTC CTCTGTGAAC
    AGCAGTGGGG AGATGTTGAA GCTGTCAGCC CCCCTGGACC ACCTCAACCT GCATCTCCGG
    GAGGGTTCTA TCCTGCCCAC ACAGAAACCC GGGACCACCA GCAAGGCCAC CCGAGGGAAC
    CCGCTGCGCC TCATTGTGGC CCTGTCCCCA CGTGCCACTG CCTGGGGGGA CCTCTTCTGG
    GATGACGGCG AGAGCCTGGA CACCTTCGAG CGGGGCAACT ACTCCTACCT GGTGTTCAAT
    GCCACAGAGA ACATCTTCAC CTCCAACGTC CTCCATGCCA GCACCGAGGC CACCTATGTC
    ACCATCGACA CGGTGAGCTT CTACGGAATG CAGGAGCCGC CCAGCAAGGT CCTCCTGGAT
    GGGCAGGAGA AGCCCTTCTC CTACCTGGAC AACCAGGTCC TCACGGTCAG CGGCCTCGGC
    CTCAGGCTCA GCCAGGGCTT CTCCCTGCAG TGGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149821.1
    CDS1619..4417
    Translation

    Target ORF information:

    RefSeq Version XM_015294335.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (GAAGSD), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294335.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACAATGA GGTCGTACCA GAAGCTGAGG ACGGCGGTGC CGCAGCTGGT GTTGAGCCAG 
    GAGGAGGAGG AGGAGAGTGC GGCCACCCCA ACACCTGCTG GCCGCAGCAA GCTGGCCCCG
    TGGTGGGTGG GCAGCGGGCT GCTGATCACC GCCGTGCTGC TGAGCACCAT CACTGTCTGG
    GTGCTGCGAC AGGTGTCCAG GGGCTGGCAC GGCCCCGCAC CACCTTCCCA ATGTCACCTG
    GTCCCTGAGA GCCACCGCTA TGACTGCTAC CCCGAAAGGA GCGTGGTGGT GACCCAGGAG
    CTGTGCGAGA GCCGGGGGTG CTGCTTCATC GAGACCCCCC CTGCAGTGGG GGGCAAGAGG
    GGGGTGCCCT GGTGCTTCTA CCCACCCAGC TTCCCCAGCT ACGTGGTGCA GAGCCTCAAC
    CAGACGGCGC TGGGCATGAC GGGGCTGCTG GTGCGCAGGG AGAAGGCCTA TTACCCCAAG
    GACATCCAGG TGCTGAGGAT GGACGTGGAG TTCCAGACGA ACACACGGCT GCGCATCAAG
    ATAACCGATG CAGCCAAGCC CCGGTATGAG GTGCCCCTGG AAGTTCCCCG AGTGATGAAG
    AGAGCAGAAA ACCCCATCTA CAGCCTGGAG TTCTCCCAGG ACCCCTTTGG GGTGCTGCTG
    AGGCGCCAAG GGACAGGGAC AGTGCTGCTC AACACCACCG TGGCTCCCCT GATCTTCGCC
    GATCAGTTCC TGCAGATCTC CACCACGCTG CCCTCCAGGT TCCTGTATGG GCTGGGGGAG
    CACCGCAGCA CTCTGCTGCA CAGCCTCGAC TGGAACACCC TCACCCTCTG GGCCCGTGAT
    GTGGCCCCCA CGGAATCCTT CAACCTGTAC GGCGCTCACC CCTTCTACCT GCTGATGGAG
    GAGGGCGGCG ATGCCCACGG GGTTTTCCTC CTCAACAGCA ACGCCATGGA GGTGGCCCTG
    CAGCCTGCCC CGGGCCTGAC TTGGAGGACC ATTGGAGGTG TGCTGGACTT CTACATCTTC
    CTGGGGCCTG ACCCCAACAT GGTCATCCAG CAGTACCAGG AGGTGATAGG TTTCCCAGCC
    ATGCCTCCAC TCTGGGCGCT CGGCTTCCAC CTCTGCCGCT GGGGCTACGG CTCCAGCAAC
    GAGACCTGGC AGACTGCAAG GGCCATGAGG AACTTCCAGA TCCCACAGGA TGCGCAATGG
    AACGACATTG ATTACATGGA CGGGTACCGG GACTTCACCT TCGATCCCCA GAAATTCGCC
    TCCCTCCCCT CACTGGTGGA AGATCTCCAC AAGCACGGGC AGCACTACGT GATGATCTTG
    GACCCCGGCA TCAGCAGCAC CAGCCCCCAC GGCTCCTACT GGCCTTTCGA TGAAGGCTTG
    AGACGTGCTT TGTTCCTGAA CACCACCCAG GGGCAGCCAC TGATCGGGCA GGTCTGGCCT
    GGTTATACTG CCTTCCCCGA CTTCTCCAAC AGAGACACAC ATCAGTGGTG GCTGGAGAAC
    CTGCAGCGCT TCCACACCCG TGTGCCCTTC GATGGCCTCT GGATCGACAT GAACGAGCCA
    TCCAACTTCA TGGATGGGTC TGAAGAGGGC TGCCCACCCG GAGAGCTCGA CAGCCCCCCG
    TACACGCCGG CTGTGCTGGG CAATTCCCTG TCCGCCAAGA CGGTGTGTGC CTCAGCGAAG
    CAGAACGCCT CGGTGCACTA CAACCTGCAC AACCTCTACG GGCTGAAGGA AGCCGAAGCG
    ACGGCGAGCG CCTTGATCCA CATCCGAGGA AAGCGTCCCT TTGTCATCTC CCGCTCTACC
    TTCCCCAGCC AGGGCCGGTA CTCGGGTCAC TGGCTGGGTG ACAACCGGAG CCAGTGGAAG
    GACATGTATT ACTCCATCCC AGGGATGCTG AGCTTCAGCC TGTTTGGGAT CCCACTGGTG
    GGGGCCGACA TCTGCGGCTT CTCCGGCAGC ACCTCGGAGG AGCTGTGCAC CCGCTGGATG
    CAGCTCGGGG CCTTCTACCC CTTCTCCCGC AACCACAACA CCCAGAACGA GAAGGCCCAG
    GACCCCACAG CTTTCAGCCC CTCGGCGAGG ACAGCCATGA AGGACGTACT GCTCACCCGC
    TACTCCCTCC TGCCTTTCCT CTACACACTT TTCCACCGTG CCCACCTGCA AGGGGAAACC
    GTTGCCCGAC CTTTGTTCTT TGAGTTCCCC TGGGATGTGG CCACGTATGG GCTGGACAGG
    CAGTTCCTGT GGGGCCAGAG CCTGCTGGTG ACACCGGTGC TGGAGCCCGG AGCAGACTCG
    GTGTTGGGCT ATTTCCCCCG AGGCGTGTGG TACGACTTCT ACACGGGCTC CTCTGTGAAC
    AGCAGTGGGG AGATGTTGAA GCTGTCAGCC CCCCTGGACC ACCTCAACCT GCATCTCCGG
    GAGGGTTCTA TCCTGCCCAC ACAGAAACCC GGGACCACCA GCAAGGCCAC CCGAGGGAAC
    CCGCTGCGCC TCATTGTGGC CCTGTCCCCA CGTGCCACTG CCTGGGGGGA CCTCTTCTGG
    GATGACGGCG AGAGCCTGGA CACCTTCGAG CGGGGCAACT ACTCCTACCT GGTGTTCAAT
    GCCACAGAGA ACATCTTCAC CTCCAACGTC CTCCATGCCA GCACCGAGGC CACCTATGTC
    ACCATCGACA CGGTGAGCTT CTACGGAATG CAGGAGCCGC CCAGCAAGGT CCTCCTGGAT
    GGGCAGGAGA AGCCCTTCTC CTACCTGGAC AACCAGGTCC TCACGGTCAG CGGCCTCGGC
    CTCAGGCTCA GCCAGGGCTT CTCCCTGCAG TGGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42301
    Clone ID Related Accession (Same CDS sequence) XM_015294336.1 , XM_015294337.1 , XM_015294336.2 , XM_015294337.2
    Accession Version XM_015294336.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2793bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product lysosomal alpha-glucosidase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGTCGT ACCAGAAGCT GAGGACGGCG GTGCCGCAGC TGGTGTTGAG CCAGGAGGAG 
    GAGGAGGAGA GTGCGGCCAC CCCAACACCT GCTGGCCGCA GCAAGCTGGC CCCGTGGTGG
    GTGGGCAGCG GGCTGCTGAT CACCGCCGTG CTGCTGAGCA CCATCACTGT CTGGGTGCTG
    CGACAGGTGT CCAGGGGCTG GCACGGCCCC GCACCACCTT CCCAATGTCA CCTGGTCCCT
    GAGAGCCACC GCTATGACTG CTACCCCGAA AGGAGCGTGG TGGTGACCCA GGAGCTGTGC
    GAGAGCCGGG GGTGCTGCTT CATCGAGACC CCCCCTGCAG TGGGGGGCAA GAGGGGGGTG
    CCCTGGTGCT TCTACCCACC CAGCTTCCCC AGCTACGTGG TGCAGAGCCT CAACCAGACG
    GCGCTGGGCA TGACGGGGCT GCTGGTGCGC AGGGAGAAGG CCTATTACCC CAAGGACATC
    CAGGTGCTGA GGATGGACGT GGAGTTCCAG ACGAACACAC GGCTGCGCAT CAAGATAACC
    GATGCAGCCA AGCCCCGGTA TGAGGTGCCC CTGGAAGTTC CCCGAGTGAT GAAGAGAGCA
    GAAAACCCCA TCTACAGCCT GGAGTTCTCC CAGGACCCCT TTGGGGTGCT GCTGAGGCGC
    CAAGGGACAG GGACAGTGCT GCTCAACACC ACCGTGGCTC CCCTGATCTT CGCCGATCAG
    TTCCTGCAGA TCTCCACCAC GCTGCCCTCC AGGTTCCTGT ATGGGCTGGG GGAGCACCGC
    AGCACTCTGC TGCACAGCCT CGACTGGAAC ACCCTCACCC TCTGGGCCCG TGATGTGGCC
    CCCACGGAAT CCTTCAACCT GTACGGCGCT CACCCCTTCT ACCTGCTGAT GGAGGAGGGC
    GGCGATGCCC ACGGGGTTTT CCTCCTCAAC AGCAACGCCA TGGAGGTGGC CCTGCAGCCT
    GCCCCGGGCC TGACTTGGAG GACCATTGGA GGTGTGCTGG ACTTCTACAT CTTCCTGGGG
    CCTGACCCCA ACATGGTCAT CCAGCAGTAC CAGGAGGTGA TAGGTTTCCC AGCCATGCCT
    CCACTCTGGG CGCTCGGCTT CCACCTCTGC CGCTGGGGCT ACGGCTCCAG CAACGAGACC
    TGGCAGACTG CAAGGGCCAT GAGGAACTTC CAGATCCCAC AGGATGCGCA ATGGAACGAC
    ATTGATTACA TGGACGGGTA CCGGGACTTC ACCTTCGATC CCCAGAAATT CGCCTCCCTC
    CCCTCACTGG TGGAAGATCT CCACAAGCAC GGGCAGCACT ACGTGATGAT CTTGGACCCC
    GGCATCAGCA GCACCAGCCC CCACGGCTCC TACTGGCCTT TCGATGAAGG CTTGAGACGT
    GCTTTGTTCC TGAACACCAC CCAGGGGCAG CCACTGATCG GGCAGGTCTG GCCTGGTTAT
    ACTGCCTTCC CCGACTTCTC CAACAGAGAC ACACATCAGT GGTGGCTGGA GAACCTGCAG
    CGCTTCCACA CCCGTGTGCC CTTCGATGGC CTCTGGATCG ACATGAACGA GCCATCCAAC
    TTCATGGATG GGTCTGAAGA GGGCTGCCCA CCCGGAGAGC TCGACAGCCC CCCGTACACG
    CCGGCTGTGC TGGGCAATTC CCTGTCCGCC AAGACGGTGT GTGCCTCAGC GAAGCAGAAC
    GCCTCGGTGC ACTACAACCT GCACAACCTC TACGGGCTGA AGGAAGCCGA AGCGACGGCG
    AGCGCCTTGA TCCACATCCG AGGAAAGCGT CCCTTTGTCA TCTCCCGCTC TACCTTCCCC
    AGCCAGGGCC GGTACTCGGG TCACTGGCTG GGTGACAACC GGAGCCAGTG GAAGGACATG
    TATTACTCCA TCCCAGGGAT GCTGAGCTTC AGCCTGTTTG GGATCCCACT GGTGGGGGCC
    GACATCTGCG GCTTCTCCGG CAGCACCTCG GAGGAGCTGT GCACCCGCTG GATGCAGCTC
    GGGGCCTTCT ACCCCTTCTC CCGCAACCAC AACACCCAGA ACGAGAAGGC CCAGGACCCC
    ACAGCTTTCA GCCCCTCGGC GAGGACAGCC ATGAAGGACG TACTGCTCAC CCGCTACTCC
    CTCCTGCCTT TCCTCTACAC ACTTTTCCAC CGTGCCCACC TGCAAGGGGA AACCGTTGCC
    CGACCTTTGT TCTTTGAGTT CCCCTGGGAT GTGGCCACGT ATGGGCTGGA CAGGCAGTTC
    CTGTGGGGCC AGAGCCTGCT GGTGACACCG GTGCTGGAGC CCGGAGCAGA CTCGGTGTTG
    GGCTATTTCC CCCGAGGCGT GTGGTACGAC TTCTACACGG GCTCCTCTGT GAACAGCAGT
    GGGGAGATGT TGAAGCTGTC AGCCCCCCTG GACCACCTCA ACCTGCATCT CCGGGAGGGT
    TCTATCCTGC CCACACAGAA ACCCGGGACC ACCAGCAAGG CCACCCGAGG GAACCCGCTG
    CGCCTCATTG TGGCCCTGTC CCCACGTGCC ACTGCCTGGG GGGACCTCTT CTGGGATGAC
    GGCGAGAGCC TGGACACCTT CGAGCGGGGC AACTACTCCT ACCTGGTGTT CAATGCCACA
    GAGAACATCT TCACCTCCAA CGTCCTCCAT GCCAGCACCG AGGCCACCTA TGTCACCATC
    GACACGGTGA GCTTCTACGG AATGCAGGAG CCGCCCAGCA AGGTCCTCCT GGATGGGCAG
    GAGAAGCCCT TCTCCTACCT GGACAACCAG GTCCTCACGG TCAGCGGCCT CGGCCTCAGG
    CTCAGCCAGG GCTTCTCCCT GCAGTGGCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149822.1
    CDS1616..4408
    Translation

    Target ORF information:

    RefSeq Version XM_015294336.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (GAA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294336.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGTCGT ACCAGAAGCT GAGGACGGCG GTGCCGCAGC TGGTGTTGAG CCAGGAGGAG 
    GAGGAGGAGA GTGCGGCCAC CCCAACACCT GCTGGCCGCA GCAAGCTGGC CCCGTGGTGG
    GTGGGCAGCG GGCTGCTGAT CACCGCCGTG CTGCTGAGCA CCATCACTGT CTGGGTGCTG
    CGACAGGTGT CCAGGGGCTG GCACGGCCCC GCACCACCTT CCCAATGTCA CCTGGTCCCT
    GAGAGCCACC GCTATGACTG CTACCCCGAA AGGAGCGTGG TGGTGACCCA GGAGCTGTGC
    GAGAGCCGGG GGTGCTGCTT CATCGAGACC CCCCCTGCAG TGGGGGGCAA GAGGGGGGTG
    CCCTGGTGCT TCTACCCACC CAGCTTCCCC AGCTACGTGG TGCAGAGCCT CAACCAGACG
    GCGCTGGGCA TGACGGGGCT GCTGGTGCGC AGGGAGAAGG CCTATTACCC CAAGGACATC
    CAGGTGCTGA GGATGGACGT GGAGTTCCAG ACGAACACAC GGCTGCGCAT CAAGATAACC
    GATGCAGCCA AGCCCCGGTA TGAGGTGCCC CTGGAAGTTC CCCGAGTGAT GAAGAGAGCA
    GAAAACCCCA TCTACAGCCT GGAGTTCTCC CAGGACCCCT TTGGGGTGCT GCTGAGGCGC
    CAAGGGACAG GGACAGTGCT GCTCAACACC ACCGTGGCTC CCCTGATCTT CGCCGATCAG
    TTCCTGCAGA TCTCCACCAC GCTGCCCTCC AGGTTCCTGT ATGGGCTGGG GGAGCACCGC
    AGCACTCTGC TGCACAGCCT CGACTGGAAC ACCCTCACCC TCTGGGCCCG TGATGTGGCC
    CCCACGGAAT CCTTCAACCT GTACGGCGCT CACCCCTTCT ACCTGCTGAT GGAGGAGGGC
    GGCGATGCCC ACGGGGTTTT CCTCCTCAAC AGCAACGCCA TGGAGGTGGC CCTGCAGCCT
    GCCCCGGGCC TGACTTGGAG GACCATTGGA GGTGTGCTGG ACTTCTACAT CTTCCTGGGG
    CCTGACCCCA ACATGGTCAT CCAGCAGTAC CAGGAGGTGA TAGGTTTCCC AGCCATGCCT
    CCACTCTGGG CGCTCGGCTT CCACCTCTGC CGCTGGGGCT ACGGCTCCAG CAACGAGACC
    TGGCAGACTG CAAGGGCCAT GAGGAACTTC CAGATCCCAC AGGATGCGCA ATGGAACGAC
    ATTGATTACA TGGACGGGTA CCGGGACTTC ACCTTCGATC CCCAGAAATT CGCCTCCCTC
    CCCTCACTGG TGGAAGATCT CCACAAGCAC GGGCAGCACT ACGTGATGAT CTTGGACCCC
    GGCATCAGCA GCACCAGCCC CCACGGCTCC TACTGGCCTT TCGATGAAGG CTTGAGACGT
    GCTTTGTTCC TGAACACCAC CCAGGGGCAG CCACTGATCG GGCAGGTCTG GCCTGGTTAT
    ACTGCCTTCC CCGACTTCTC CAACAGAGAC ACACATCAGT GGTGGCTGGA GAACCTGCAG
    CGCTTCCACA CCCGTGTGCC CTTCGATGGC CTCTGGATCG ACATGAACGA GCCATCCAAC
    TTCATGGATG GGTCTGAAGA GGGCTGCCCA CCCGGAGAGC TCGACAGCCC CCCGTACACG
    CCGGCTGTGC TGGGCAATTC CCTGTCCGCC AAGACGGTGT GTGCCTCAGC GAAGCAGAAC
    GCCTCGGTGC ACTACAACCT GCACAACCTC TACGGGCTGA AGGAAGCCGA AGCGACGGCG
    AGCGCCTTGA TCCACATCCG AGGAAAGCGT CCCTTTGTCA TCTCCCGCTC TACCTTCCCC
    AGCCAGGGCC GGTACTCGGG TCACTGGCTG GGTGACAACC GGAGCCAGTG GAAGGACATG
    TATTACTCCA TCCCAGGGAT GCTGAGCTTC AGCCTGTTTG GGATCCCACT GGTGGGGGCC
    GACATCTGCG GCTTCTCCGG CAGCACCTCG GAGGAGCTGT GCACCCGCTG GATGCAGCTC
    GGGGCCTTCT ACCCCTTCTC CCGCAACCAC AACACCCAGA ACGAGAAGGC CCAGGACCCC
    ACAGCTTTCA GCCCCTCGGC GAGGACAGCC ATGAAGGACG TACTGCTCAC CCGCTACTCC
    CTCCTGCCTT TCCTCTACAC ACTTTTCCAC CGTGCCCACC TGCAAGGGGA AACCGTTGCC
    CGACCTTTGT TCTTTGAGTT CCCCTGGGAT GTGGCCACGT ATGGGCTGGA CAGGCAGTTC
    CTGTGGGGCC AGAGCCTGCT GGTGACACCG GTGCTGGAGC CCGGAGCAGA CTCGGTGTTG
    GGCTATTTCC CCCGAGGCGT GTGGTACGAC TTCTACACGG GCTCCTCTGT GAACAGCAGT
    GGGGAGATGT TGAAGCTGTC AGCCCCCCTG GACCACCTCA ACCTGCATCT CCGGGAGGGT
    TCTATCCTGC CCACACAGAA ACCCGGGACC ACCAGCAAGG CCACCCGAGG GAACCCGCTG
    CGCCTCATTG TGGCCCTGTC CCCACGTGCC ACTGCCTGGG GGGACCTCTT CTGGGATGAC
    GGCGAGAGCC TGGACACCTT CGAGCGGGGC AACTACTCCT ACCTGGTGTT CAATGCCACA
    GAGAACATCT TCACCTCCAA CGTCCTCCAT GCCAGCACCG AGGCCACCTA TGTCACCATC
    GACACGGTGA GCTTCTACGG AATGCAGGAG CCGCCCAGCA AGGTCCTCCT GGATGGGCAG
    GAGAAGCCCT TCTCCTACCT GGACAACCAG GTCCTCACGG TCAGCGGCCT CGGCCTCAGG
    CTCAGCCAGG GCTTCTCCCT GCAGTGGCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42301
    Clone ID Related Accession (Same CDS sequence) XM_015294336.1 , XM_015294337.1 , XM_015294336.2 , XM_015294337.2
    Accession Version XM_015294337.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2793bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product lysosomal alpha-glucosidase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGTCGT ACCAGAAGCT GAGGACGGCG GTGCCGCAGC TGGTGTTGAG CCAGGAGGAG 
    GAGGAGGAGA GTGCGGCCAC CCCAACACCT GCTGGCCGCA GCAAGCTGGC CCCGTGGTGG
    GTGGGCAGCG GGCTGCTGAT CACCGCCGTG CTGCTGAGCA CCATCACTGT CTGGGTGCTG
    CGACAGGTGT CCAGGGGCTG GCACGGCCCC GCACCACCTT CCCAATGTCA CCTGGTCCCT
    GAGAGCCACC GCTATGACTG CTACCCCGAA AGGAGCGTGG TGGTGACCCA GGAGCTGTGC
    GAGAGCCGGG GGTGCTGCTT CATCGAGACC CCCCCTGCAG TGGGGGGCAA GAGGGGGGTG
    CCCTGGTGCT TCTACCCACC CAGCTTCCCC AGCTACGTGG TGCAGAGCCT CAACCAGACG
    GCGCTGGGCA TGACGGGGCT GCTGGTGCGC AGGGAGAAGG CCTATTACCC CAAGGACATC
    CAGGTGCTGA GGATGGACGT GGAGTTCCAG ACGAACACAC GGCTGCGCAT CAAGATAACC
    GATGCAGCCA AGCCCCGGTA TGAGGTGCCC CTGGAAGTTC CCCGAGTGAT GAAGAGAGCA
    GAAAACCCCA TCTACAGCCT GGAGTTCTCC CAGGACCCCT TTGGGGTGCT GCTGAGGCGC
    CAAGGGACAG GGACAGTGCT GCTCAACACC ACCGTGGCTC CCCTGATCTT CGCCGATCAG
    TTCCTGCAGA TCTCCACCAC GCTGCCCTCC AGGTTCCTGT ATGGGCTGGG GGAGCACCGC
    AGCACTCTGC TGCACAGCCT CGACTGGAAC ACCCTCACCC TCTGGGCCCG TGATGTGGCC
    CCCACGGAAT CCTTCAACCT GTACGGCGCT CACCCCTTCT ACCTGCTGAT GGAGGAGGGC
    GGCGATGCCC ACGGGGTTTT CCTCCTCAAC AGCAACGCCA TGGAGGTGGC CCTGCAGCCT
    GCCCCGGGCC TGACTTGGAG GACCATTGGA GGTGTGCTGG ACTTCTACAT CTTCCTGGGG
    CCTGACCCCA ACATGGTCAT CCAGCAGTAC CAGGAGGTGA TAGGTTTCCC AGCCATGCCT
    CCACTCTGGG CGCTCGGCTT CCACCTCTGC CGCTGGGGCT ACGGCTCCAG CAACGAGACC
    TGGCAGACTG CAAGGGCCAT GAGGAACTTC CAGATCCCAC AGGATGCGCA ATGGAACGAC
    ATTGATTACA TGGACGGGTA CCGGGACTTC ACCTTCGATC CCCAGAAATT CGCCTCCCTC
    CCCTCACTGG TGGAAGATCT CCACAAGCAC GGGCAGCACT ACGTGATGAT CTTGGACCCC
    GGCATCAGCA GCACCAGCCC CCACGGCTCC TACTGGCCTT TCGATGAAGG CTTGAGACGT
    GCTTTGTTCC TGAACACCAC CCAGGGGCAG CCACTGATCG GGCAGGTCTG GCCTGGTTAT
    ACTGCCTTCC CCGACTTCTC CAACAGAGAC ACACATCAGT GGTGGCTGGA GAACCTGCAG
    CGCTTCCACA CCCGTGTGCC CTTCGATGGC CTCTGGATCG ACATGAACGA GCCATCCAAC
    TTCATGGATG GGTCTGAAGA GGGCTGCCCA CCCGGAGAGC TCGACAGCCC CCCGTACACG
    CCGGCTGTGC TGGGCAATTC CCTGTCCGCC AAGACGGTGT GTGCCTCAGC GAAGCAGAAC
    GCCTCGGTGC ACTACAACCT GCACAACCTC TACGGGCTGA AGGAAGCCGA AGCGACGGCG
    AGCGCCTTGA TCCACATCCG AGGAAAGCGT CCCTTTGTCA TCTCCCGCTC TACCTTCCCC
    AGCCAGGGCC GGTACTCGGG TCACTGGCTG GGTGACAACC GGAGCCAGTG GAAGGACATG
    TATTACTCCA TCCCAGGGAT GCTGAGCTTC AGCCTGTTTG GGATCCCACT GGTGGGGGCC
    GACATCTGCG GCTTCTCCGG CAGCACCTCG GAGGAGCTGT GCACCCGCTG GATGCAGCTC
    GGGGCCTTCT ACCCCTTCTC CCGCAACCAC AACACCCAGA ACGAGAAGGC CCAGGACCCC
    ACAGCTTTCA GCCCCTCGGC GAGGACAGCC ATGAAGGACG TACTGCTCAC CCGCTACTCC
    CTCCTGCCTT TCCTCTACAC ACTTTTCCAC CGTGCCCACC TGCAAGGGGA AACCGTTGCC
    CGACCTTTGT TCTTTGAGTT CCCCTGGGAT GTGGCCACGT ATGGGCTGGA CAGGCAGTTC
    CTGTGGGGCC AGAGCCTGCT GGTGACACCG GTGCTGGAGC CCGGAGCAGA CTCGGTGTTG
    GGCTATTTCC CCCGAGGCGT GTGGTACGAC TTCTACACGG GCTCCTCTGT GAACAGCAGT
    GGGGAGATGT TGAAGCTGTC AGCCCCCCTG GACCACCTCA ACCTGCATCT CCGGGAGGGT
    TCTATCCTGC CCACACAGAA ACCCGGGACC ACCAGCAAGG CCACCCGAGG GAACCCGCTG
    CGCCTCATTG TGGCCCTGTC CCCACGTGCC ACTGCCTGGG GGGACCTCTT CTGGGATGAC
    GGCGAGAGCC TGGACACCTT CGAGCGGGGC AACTACTCCT ACCTGGTGTT CAATGCCACA
    GAGAACATCT TCACCTCCAA CGTCCTCCAT GCCAGCACCG AGGCCACCTA TGTCACCATC
    GACACGGTGA GCTTCTACGG AATGCAGGAG CCGCCCAGCA AGGTCCTCCT GGATGGGCAG
    GAGAAGCCCT TCTCCTACCT GGACAACCAG GTCCTCACGG TCAGCGGCCT CGGCCTCAGG
    CTCAGCCAGG GCTTCTCCCT GCAGTGGCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149823.1
    CDS196..2988
    Translation

    Target ORF information:

    RefSeq Version XM_015294337.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (GAA), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294337.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGTCGT ACCAGAAGCT GAGGACGGCG GTGCCGCAGC TGGTGTTGAG CCAGGAGGAG 
    GAGGAGGAGA GTGCGGCCAC CCCAACACCT GCTGGCCGCA GCAAGCTGGC CCCGTGGTGG
    GTGGGCAGCG GGCTGCTGAT CACCGCCGTG CTGCTGAGCA CCATCACTGT CTGGGTGCTG
    CGACAGGTGT CCAGGGGCTG GCACGGCCCC GCACCACCTT CCCAATGTCA CCTGGTCCCT
    GAGAGCCACC GCTATGACTG CTACCCCGAA AGGAGCGTGG TGGTGACCCA GGAGCTGTGC
    GAGAGCCGGG GGTGCTGCTT CATCGAGACC CCCCCTGCAG TGGGGGGCAA GAGGGGGGTG
    CCCTGGTGCT TCTACCCACC CAGCTTCCCC AGCTACGTGG TGCAGAGCCT CAACCAGACG
    GCGCTGGGCA TGACGGGGCT GCTGGTGCGC AGGGAGAAGG CCTATTACCC CAAGGACATC
    CAGGTGCTGA GGATGGACGT GGAGTTCCAG ACGAACACAC GGCTGCGCAT CAAGATAACC
    GATGCAGCCA AGCCCCGGTA TGAGGTGCCC CTGGAAGTTC CCCGAGTGAT GAAGAGAGCA
    GAAAACCCCA TCTACAGCCT GGAGTTCTCC CAGGACCCCT TTGGGGTGCT GCTGAGGCGC
    CAAGGGACAG GGACAGTGCT GCTCAACACC ACCGTGGCTC CCCTGATCTT CGCCGATCAG
    TTCCTGCAGA TCTCCACCAC GCTGCCCTCC AGGTTCCTGT ATGGGCTGGG GGAGCACCGC
    AGCACTCTGC TGCACAGCCT CGACTGGAAC ACCCTCACCC TCTGGGCCCG TGATGTGGCC
    CCCACGGAAT CCTTCAACCT GTACGGCGCT CACCCCTTCT ACCTGCTGAT GGAGGAGGGC
    GGCGATGCCC ACGGGGTTTT CCTCCTCAAC AGCAACGCCA TGGAGGTGGC CCTGCAGCCT
    GCCCCGGGCC TGACTTGGAG GACCATTGGA GGTGTGCTGG ACTTCTACAT CTTCCTGGGG
    CCTGACCCCA ACATGGTCAT CCAGCAGTAC CAGGAGGTGA TAGGTTTCCC AGCCATGCCT
    CCACTCTGGG CGCTCGGCTT CCACCTCTGC CGCTGGGGCT ACGGCTCCAG CAACGAGACC
    TGGCAGACTG CAAGGGCCAT GAGGAACTTC CAGATCCCAC AGGATGCGCA ATGGAACGAC
    ATTGATTACA TGGACGGGTA CCGGGACTTC ACCTTCGATC CCCAGAAATT CGCCTCCCTC
    CCCTCACTGG TGGAAGATCT CCACAAGCAC GGGCAGCACT ACGTGATGAT CTTGGACCCC
    GGCATCAGCA GCACCAGCCC CCACGGCTCC TACTGGCCTT TCGATGAAGG CTTGAGACGT
    GCTTTGTTCC TGAACACCAC CCAGGGGCAG CCACTGATCG GGCAGGTCTG GCCTGGTTAT
    ACTGCCTTCC CCGACTTCTC CAACAGAGAC ACACATCAGT GGTGGCTGGA GAACCTGCAG
    CGCTTCCACA CCCGTGTGCC CTTCGATGGC CTCTGGATCG ACATGAACGA GCCATCCAAC
    TTCATGGATG GGTCTGAAGA GGGCTGCCCA CCCGGAGAGC TCGACAGCCC CCCGTACACG
    CCGGCTGTGC TGGGCAATTC CCTGTCCGCC AAGACGGTGT GTGCCTCAGC GAAGCAGAAC
    GCCTCGGTGC ACTACAACCT GCACAACCTC TACGGGCTGA AGGAAGCCGA AGCGACGGCG
    AGCGCCTTGA TCCACATCCG AGGAAAGCGT CCCTTTGTCA TCTCCCGCTC TACCTTCCCC
    AGCCAGGGCC GGTACTCGGG TCACTGGCTG GGTGACAACC GGAGCCAGTG GAAGGACATG
    TATTACTCCA TCCCAGGGAT GCTGAGCTTC AGCCTGTTTG GGATCCCACT GGTGGGGGCC
    GACATCTGCG GCTTCTCCGG CAGCACCTCG GAGGAGCTGT GCACCCGCTG GATGCAGCTC
    GGGGCCTTCT ACCCCTTCTC CCGCAACCAC AACACCCAGA ACGAGAAGGC CCAGGACCCC
    ACAGCTTTCA GCCCCTCGGC GAGGACAGCC ATGAAGGACG TACTGCTCAC CCGCTACTCC
    CTCCTGCCTT TCCTCTACAC ACTTTTCCAC CGTGCCCACC TGCAAGGGGA AACCGTTGCC
    CGACCTTTGT TCTTTGAGTT CCCCTGGGAT GTGGCCACGT ATGGGCTGGA CAGGCAGTTC
    CTGTGGGGCC AGAGCCTGCT GGTGACACCG GTGCTGGAGC CCGGAGCAGA CTCGGTGTTG
    GGCTATTTCC CCCGAGGCGT GTGGTACGAC TTCTACACGG GCTCCTCTGT GAACAGCAGT
    GGGGAGATGT TGAAGCTGTC AGCCCCCCTG GACCACCTCA ACCTGCATCT CCGGGAGGGT
    TCTATCCTGC CCACACAGAA ACCCGGGACC ACCAGCAAGG CCACCCGAGG GAACCCGCTG
    CGCCTCATTG TGGCCCTGTC CCCACGTGCC ACTGCCTGGG GGGACCTCTT CTGGGATGAC
    GGCGAGAGCC TGGACACCTT CGAGCGGGGC AACTACTCCT ACCTGGTGTT CAATGCCACA
    GAGAACATCT TCACCTCCAA CGTCCTCCAT GCCAGCACCG AGGCCACCTA TGTCACCATC
    GACACGGTGA GCTTCTACGG AATGCAGGAG CCGCCCAGCA AGGTCCTCCT GGATGGGCAG
    GAGAAGCCCT TCTCCTACCT GGACAACCAG GTCCTCACGG TCAGCGGCCT CGGCCTCAGG
    CTCAGCCAGG GCTTCTCCCT GCAGTGGCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42301
    Clone ID Related Accession (Same CDS sequence) XM_015294336.1 , XM_015294337.1 , XM_015294336.2 , XM_015294337.2
    Accession Version XM_015294336.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2793bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product lysosomal alpha-glucosidase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294336.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGTCGT ACCAGAAGCT GAGGACGGCG GTGCCGCAGC TGGTGTTGAG CCAGGAGGAG 
    GAGGAGGAGA GTGCGGCCAC CCCAACACCT GCTGGCCGCA GCAAGCTGGC CCCGTGGTGG
    GTGGGCAGCG GGCTGCTGAT CACCGCCGTG CTGCTGAGCA CCATCACTGT CTGGGTGCTG
    CGACAGGTGT CCAGGGGCTG GCACGGCCCC GCACCACCTT CCCAATGTCA CCTGGTCCCT
    GAGAGCCACC GCTATGACTG CTACCCCGAA AGGAGCGTGG TGGTGACCCA GGAGCTGTGC
    GAGAGCCGGG GGTGCTGCTT CATCGAGACC CCCCCTGCAG TGGGGGGCAA GAGGGGGGTG
    CCCTGGTGCT TCTACCCACC CAGCTTCCCC AGCTACGTGG TGCAGAGCCT CAACCAGACG
    GCGCTGGGCA TGACGGGGCT GCTGGTGCGC AGGGAGAAGG CCTATTACCC CAAGGACATC
    CAGGTGCTGA GGATGGACGT GGAGTTCCAG ACGAACACAC GGCTGCGCAT CAAGATAACC
    GATGCAGCCA AGCCCCGGTA TGAGGTGCCC CTGGAAGTTC CCCGAGTGAT GAAGAGAGCA
    GAAAACCCCA TCTACAGCCT GGAGTTCTCC CAGGACCCCT TTGGGGTGCT GCTGAGGCGC
    CAAGGGACAG GGACAGTGCT GCTCAACACC ACCGTGGCTC CCCTGATCTT CGCCGATCAG
    TTCCTGCAGA TCTCCACCAC GCTGCCCTCC AGGTTCCTGT ATGGGCTGGG GGAGCACCGC
    AGCACTCTGC TGCACAGCCT CGACTGGAAC ACCCTCACCC TCTGGGCCCG TGATGTGGCC
    CCCACGGAAT CCTTCAACCT GTACGGCGCT CACCCCTTCT ACCTGCTGAT GGAGGAGGGC
    GGCGATGCCC ACGGGGTTTT CCTCCTCAAC AGCAACGCCA TGGAGGTGGC CCTGCAGCCT
    GCCCCGGGCC TGACTTGGAG GACCATTGGA GGTGTGCTGG ACTTCTACAT CTTCCTGGGG
    CCTGACCCCA ACATGGTCAT CCAGCAGTAC CAGGAGGTGA TAGGTTTCCC AGCCATGCCT
    CCACTCTGGG CGCTCGGCTT CCACCTCTGC CGCTGGGGCT ACGGCTCCAG CAACGAGACC
    TGGCAGACTG CAAGGGCCAT GAGGAACTTC CAGATCCCAC AGGATGCGCA ATGGAACGAC
    ATTGATTACA TGGACGGGTA CCGGGACTTC ACCTTCGATC CCCAGAAATT CGCCTCCCTC
    CCCTCACTGG TGGAAGATCT CCACAAGCAC GGGCAGCACT ACGTGATGAT CTTGGACCCC
    GGCATCAGCA GCACCAGCCC CCACGGCTCC TACTGGCCTT TCGATGAAGG CTTGAGACGT
    GCTTTGTTCC TGAACACCAC CCAGGGGCAG CCACTGATCG GGCAGGTCTG GCCTGGTTAT
    ACTGCCTTCC CCGACTTCTC CAACAGAGAC ACACATCAGT GGTGGCTGGA GAACCTGCAG
    CGCTTCCACA CCCGTGTGCC CTTCGATGGC CTCTGGATCG ACATGAACGA GCCATCCAAC
    TTCATGGATG GGTCTGAAGA GGGCTGCCCA CCCGGAGAGC TCGACAGCCC CCCGTACACG
    CCGGCTGTGC TGGGCAATTC CCTGTCCGCC AAGACGGTGT GTGCCTCAGC GAAGCAGAAC
    GCCTCGGTGC ACTACAACCT GCACAACCTC TACGGGCTGA AGGAAGCCGA AGCGACGGCG
    AGCGCCTTGA TCCACATCCG AGGAAAGCGT CCCTTTGTCA TCTCCCGCTC TACCTTCCCC
    AGCCAGGGCC GGTACTCGGG TCACTGGCTG GGTGACAACC GGAGCCAGTG GAAGGACATG
    TATTACTCCA TCCCAGGGAT GCTGAGCTTC AGCCTGTTTG GGATCCCACT GGTGGGGGCC
    GACATCTGCG GCTTCTCCGG CAGCACCTCG GAGGAGCTGT GCACCCGCTG GATGCAGCTC
    GGGGCCTTCT ACCCCTTCTC CCGCAACCAC AACACCCAGA ACGAGAAGGC CCAGGACCCC
    ACAGCTTTCA GCCCCTCGGC GAGGACAGCC ATGAAGGACG TACTGCTCAC CCGCTACTCC
    CTCCTGCCTT TCCTCTACAC ACTTTTCCAC CGTGCCCACC TGCAAGGGGA AACCGTTGCC
    CGACCTTTGT TCTTTGAGTT CCCCTGGGAT GTGGCCACGT ATGGGCTGGA CAGGCAGTTC
    CTGTGGGGCC AGAGCCTGCT GGTGACACCG GTGCTGGAGC CCGGAGCAGA CTCGGTGTTG
    GGCTATTTCC CCCGAGGCGT GTGGTACGAC TTCTACACGG GCTCCTCTGT GAACAGCAGT
    GGGGAGATGT TGAAGCTGTC AGCCCCCCTG GACCACCTCA ACCTGCATCT CCGGGAGGGT
    TCTATCCTGC CCACACAGAA ACCCGGGACC ACCAGCAAGG CCACCCGAGG GAACCCGCTG
    CGCCTCATTG TGGCCCTGTC CCCACGTGCC ACTGCCTGGG GGGACCTCTT CTGGGATGAC
    GGCGAGAGCC TGGACACCTT CGAGCGGGGC AACTACTCCT ACCTGGTGTT CAATGCCACA
    GAGAACATCT TCACCTCCAA CGTCCTCCAT GCCAGCACCG AGGCCACCTA TGTCACCATC
    GACACGGTGA GCTTCTACGG AATGCAGGAG CCGCCCAGCA AGGTCCTCCT GGATGGGCAG
    GAGAAGCCCT TCTCCTACCT GGACAACCAG GTCCTCACGG TCAGCGGCCT CGGCCTCAGG
    CTCAGCCAGG GCTTCTCCCT GCAGTGGCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149822.1
    CDS91..2883
    Translation

    Target ORF information:

    RefSeq Version XM_015294336.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (GAAGSD), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294336.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGTCGT ACCAGAAGCT GAGGACGGCG GTGCCGCAGC TGGTGTTGAG CCAGGAGGAG 
    GAGGAGGAGA GTGCGGCCAC CCCAACACCT GCTGGCCGCA GCAAGCTGGC CCCGTGGTGG
    GTGGGCAGCG GGCTGCTGAT CACCGCCGTG CTGCTGAGCA CCATCACTGT CTGGGTGCTG
    CGACAGGTGT CCAGGGGCTG GCACGGCCCC GCACCACCTT CCCAATGTCA CCTGGTCCCT
    GAGAGCCACC GCTATGACTG CTACCCCGAA AGGAGCGTGG TGGTGACCCA GGAGCTGTGC
    GAGAGCCGGG GGTGCTGCTT CATCGAGACC CCCCCTGCAG TGGGGGGCAA GAGGGGGGTG
    CCCTGGTGCT TCTACCCACC CAGCTTCCCC AGCTACGTGG TGCAGAGCCT CAACCAGACG
    GCGCTGGGCA TGACGGGGCT GCTGGTGCGC AGGGAGAAGG CCTATTACCC CAAGGACATC
    CAGGTGCTGA GGATGGACGT GGAGTTCCAG ACGAACACAC GGCTGCGCAT CAAGATAACC
    GATGCAGCCA AGCCCCGGTA TGAGGTGCCC CTGGAAGTTC CCCGAGTGAT GAAGAGAGCA
    GAAAACCCCA TCTACAGCCT GGAGTTCTCC CAGGACCCCT TTGGGGTGCT GCTGAGGCGC
    CAAGGGACAG GGACAGTGCT GCTCAACACC ACCGTGGCTC CCCTGATCTT CGCCGATCAG
    TTCCTGCAGA TCTCCACCAC GCTGCCCTCC AGGTTCCTGT ATGGGCTGGG GGAGCACCGC
    AGCACTCTGC TGCACAGCCT CGACTGGAAC ACCCTCACCC TCTGGGCCCG TGATGTGGCC
    CCCACGGAAT CCTTCAACCT GTACGGCGCT CACCCCTTCT ACCTGCTGAT GGAGGAGGGC
    GGCGATGCCC ACGGGGTTTT CCTCCTCAAC AGCAACGCCA TGGAGGTGGC CCTGCAGCCT
    GCCCCGGGCC TGACTTGGAG GACCATTGGA GGTGTGCTGG ACTTCTACAT CTTCCTGGGG
    CCTGACCCCA ACATGGTCAT CCAGCAGTAC CAGGAGGTGA TAGGTTTCCC AGCCATGCCT
    CCACTCTGGG CGCTCGGCTT CCACCTCTGC CGCTGGGGCT ACGGCTCCAG CAACGAGACC
    TGGCAGACTG CAAGGGCCAT GAGGAACTTC CAGATCCCAC AGGATGCGCA ATGGAACGAC
    ATTGATTACA TGGACGGGTA CCGGGACTTC ACCTTCGATC CCCAGAAATT CGCCTCCCTC
    CCCTCACTGG TGGAAGATCT CCACAAGCAC GGGCAGCACT ACGTGATGAT CTTGGACCCC
    GGCATCAGCA GCACCAGCCC CCACGGCTCC TACTGGCCTT TCGATGAAGG CTTGAGACGT
    GCTTTGTTCC TGAACACCAC CCAGGGGCAG CCACTGATCG GGCAGGTCTG GCCTGGTTAT
    ACTGCCTTCC CCGACTTCTC CAACAGAGAC ACACATCAGT GGTGGCTGGA GAACCTGCAG
    CGCTTCCACA CCCGTGTGCC CTTCGATGGC CTCTGGATCG ACATGAACGA GCCATCCAAC
    TTCATGGATG GGTCTGAAGA GGGCTGCCCA CCCGGAGAGC TCGACAGCCC CCCGTACACG
    CCGGCTGTGC TGGGCAATTC CCTGTCCGCC AAGACGGTGT GTGCCTCAGC GAAGCAGAAC
    GCCTCGGTGC ACTACAACCT GCACAACCTC TACGGGCTGA AGGAAGCCGA AGCGACGGCG
    AGCGCCTTGA TCCACATCCG AGGAAAGCGT CCCTTTGTCA TCTCCCGCTC TACCTTCCCC
    AGCCAGGGCC GGTACTCGGG TCACTGGCTG GGTGACAACC GGAGCCAGTG GAAGGACATG
    TATTACTCCA TCCCAGGGAT GCTGAGCTTC AGCCTGTTTG GGATCCCACT GGTGGGGGCC
    GACATCTGCG GCTTCTCCGG CAGCACCTCG GAGGAGCTGT GCACCCGCTG GATGCAGCTC
    GGGGCCTTCT ACCCCTTCTC CCGCAACCAC AACACCCAGA ACGAGAAGGC CCAGGACCCC
    ACAGCTTTCA GCCCCTCGGC GAGGACAGCC ATGAAGGACG TACTGCTCAC CCGCTACTCC
    CTCCTGCCTT TCCTCTACAC ACTTTTCCAC CGTGCCCACC TGCAAGGGGA AACCGTTGCC
    CGACCTTTGT TCTTTGAGTT CCCCTGGGAT GTGGCCACGT ATGGGCTGGA CAGGCAGTTC
    CTGTGGGGCC AGAGCCTGCT GGTGACACCG GTGCTGGAGC CCGGAGCAGA CTCGGTGTTG
    GGCTATTTCC CCCGAGGCGT GTGGTACGAC TTCTACACGG GCTCCTCTGT GAACAGCAGT
    GGGGAGATGT TGAAGCTGTC AGCCCCCCTG GACCACCTCA ACCTGCATCT CCGGGAGGGT
    TCTATCCTGC CCACACAGAA ACCCGGGACC ACCAGCAAGG CCACCCGAGG GAACCCGCTG
    CGCCTCATTG TGGCCCTGTC CCCACGTGCC ACTGCCTGGG GGGACCTCTT CTGGGATGAC
    GGCGAGAGCC TGGACACCTT CGAGCGGGGC AACTACTCCT ACCTGGTGTT CAATGCCACA
    GAGAACATCT TCACCTCCAA CGTCCTCCAT GCCAGCACCG AGGCCACCTA TGTCACCATC
    GACACGGTGA GCTTCTACGG AATGCAGGAG CCGCCCAGCA AGGTCCTCCT GGATGGGCAG
    GAGAAGCCCT TCTCCTACCT GGACAACCAG GTCCTCACGG TCAGCGGCCT CGGCCTCAGG
    CTCAGCCAGG GCTTCTCCCT GCAGTGGCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42301
    Clone ID Related Accession (Same CDS sequence) XM_015294336.1 , XM_015294337.1 , XM_015294336.2 , XM_015294337.2
    Accession Version XM_015294337.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2793bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product lysosomal alpha-glucosidase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294337.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGTCGT ACCAGAAGCT GAGGACGGCG GTGCCGCAGC TGGTGTTGAG CCAGGAGGAG 
    GAGGAGGAGA GTGCGGCCAC CCCAACACCT GCTGGCCGCA GCAAGCTGGC CCCGTGGTGG
    GTGGGCAGCG GGCTGCTGAT CACCGCCGTG CTGCTGAGCA CCATCACTGT CTGGGTGCTG
    CGACAGGTGT CCAGGGGCTG GCACGGCCCC GCACCACCTT CCCAATGTCA CCTGGTCCCT
    GAGAGCCACC GCTATGACTG CTACCCCGAA AGGAGCGTGG TGGTGACCCA GGAGCTGTGC
    GAGAGCCGGG GGTGCTGCTT CATCGAGACC CCCCCTGCAG TGGGGGGCAA GAGGGGGGTG
    CCCTGGTGCT TCTACCCACC CAGCTTCCCC AGCTACGTGG TGCAGAGCCT CAACCAGACG
    GCGCTGGGCA TGACGGGGCT GCTGGTGCGC AGGGAGAAGG CCTATTACCC CAAGGACATC
    CAGGTGCTGA GGATGGACGT GGAGTTCCAG ACGAACACAC GGCTGCGCAT CAAGATAACC
    GATGCAGCCA AGCCCCGGTA TGAGGTGCCC CTGGAAGTTC CCCGAGTGAT GAAGAGAGCA
    GAAAACCCCA TCTACAGCCT GGAGTTCTCC CAGGACCCCT TTGGGGTGCT GCTGAGGCGC
    CAAGGGACAG GGACAGTGCT GCTCAACACC ACCGTGGCTC CCCTGATCTT CGCCGATCAG
    TTCCTGCAGA TCTCCACCAC GCTGCCCTCC AGGTTCCTGT ATGGGCTGGG GGAGCACCGC
    AGCACTCTGC TGCACAGCCT CGACTGGAAC ACCCTCACCC TCTGGGCCCG TGATGTGGCC
    CCCACGGAAT CCTTCAACCT GTACGGCGCT CACCCCTTCT ACCTGCTGAT GGAGGAGGGC
    GGCGATGCCC ACGGGGTTTT CCTCCTCAAC AGCAACGCCA TGGAGGTGGC CCTGCAGCCT
    GCCCCGGGCC TGACTTGGAG GACCATTGGA GGTGTGCTGG ACTTCTACAT CTTCCTGGGG
    CCTGACCCCA ACATGGTCAT CCAGCAGTAC CAGGAGGTGA TAGGTTTCCC AGCCATGCCT
    CCACTCTGGG CGCTCGGCTT CCACCTCTGC CGCTGGGGCT ACGGCTCCAG CAACGAGACC
    TGGCAGACTG CAAGGGCCAT GAGGAACTTC CAGATCCCAC AGGATGCGCA ATGGAACGAC
    ATTGATTACA TGGACGGGTA CCGGGACTTC ACCTTCGATC CCCAGAAATT CGCCTCCCTC
    CCCTCACTGG TGGAAGATCT CCACAAGCAC GGGCAGCACT ACGTGATGAT CTTGGACCCC
    GGCATCAGCA GCACCAGCCC CCACGGCTCC TACTGGCCTT TCGATGAAGG CTTGAGACGT
    GCTTTGTTCC TGAACACCAC CCAGGGGCAG CCACTGATCG GGCAGGTCTG GCCTGGTTAT
    ACTGCCTTCC CCGACTTCTC CAACAGAGAC ACACATCAGT GGTGGCTGGA GAACCTGCAG
    CGCTTCCACA CCCGTGTGCC CTTCGATGGC CTCTGGATCG ACATGAACGA GCCATCCAAC
    TTCATGGATG GGTCTGAAGA GGGCTGCCCA CCCGGAGAGC TCGACAGCCC CCCGTACACG
    CCGGCTGTGC TGGGCAATTC CCTGTCCGCC AAGACGGTGT GTGCCTCAGC GAAGCAGAAC
    GCCTCGGTGC ACTACAACCT GCACAACCTC TACGGGCTGA AGGAAGCCGA AGCGACGGCG
    AGCGCCTTGA TCCACATCCG AGGAAAGCGT CCCTTTGTCA TCTCCCGCTC TACCTTCCCC
    AGCCAGGGCC GGTACTCGGG TCACTGGCTG GGTGACAACC GGAGCCAGTG GAAGGACATG
    TATTACTCCA TCCCAGGGAT GCTGAGCTTC AGCCTGTTTG GGATCCCACT GGTGGGGGCC
    GACATCTGCG GCTTCTCCGG CAGCACCTCG GAGGAGCTGT GCACCCGCTG GATGCAGCTC
    GGGGCCTTCT ACCCCTTCTC CCGCAACCAC AACACCCAGA ACGAGAAGGC CCAGGACCCC
    ACAGCTTTCA GCCCCTCGGC GAGGACAGCC ATGAAGGACG TACTGCTCAC CCGCTACTCC
    CTCCTGCCTT TCCTCTACAC ACTTTTCCAC CGTGCCCACC TGCAAGGGGA AACCGTTGCC
    CGACCTTTGT TCTTTGAGTT CCCCTGGGAT GTGGCCACGT ATGGGCTGGA CAGGCAGTTC
    CTGTGGGGCC AGAGCCTGCT GGTGACACCG GTGCTGGAGC CCGGAGCAGA CTCGGTGTTG
    GGCTATTTCC CCCGAGGCGT GTGGTACGAC TTCTACACGG GCTCCTCTGT GAACAGCAGT
    GGGGAGATGT TGAAGCTGTC AGCCCCCCTG GACCACCTCA ACCTGCATCT CCGGGAGGGT
    TCTATCCTGC CCACACAGAA ACCCGGGACC ACCAGCAAGG CCACCCGAGG GAACCCGCTG
    CGCCTCATTG TGGCCCTGTC CCCACGTGCC ACTGCCTGGG GGGACCTCTT CTGGGATGAC
    GGCGAGAGCC TGGACACCTT CGAGCGGGGC AACTACTCCT ACCTGGTGTT CAATGCCACA
    GAGAACATCT TCACCTCCAA CGTCCTCCAT GCCAGCACCG AGGCCACCTA TGTCACCATC
    GACACGGTGA GCTTCTACGG AATGCAGGAG CCGCCCAGCA AGGTCCTCCT GGATGGGCAG
    GAGAAGCCCT TCTCCTACCT GGACAACCAG GTCCTCACGG TCAGCGGCCT CGGCCTCAGG
    CTCAGCCAGG GCTTCTCCCT GCAGTGGCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149823.1
    CDS1390..4182
    Translation

    Target ORF information:

    RefSeq Version XM_015294337.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (GAAGSD), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294337.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGTCGT ACCAGAAGCT GAGGACGGCG GTGCCGCAGC TGGTGTTGAG CCAGGAGGAG 
    GAGGAGGAGA GTGCGGCCAC CCCAACACCT GCTGGCCGCA GCAAGCTGGC CCCGTGGTGG
    GTGGGCAGCG GGCTGCTGAT CACCGCCGTG CTGCTGAGCA CCATCACTGT CTGGGTGCTG
    CGACAGGTGT CCAGGGGCTG GCACGGCCCC GCACCACCTT CCCAATGTCA CCTGGTCCCT
    GAGAGCCACC GCTATGACTG CTACCCCGAA AGGAGCGTGG TGGTGACCCA GGAGCTGTGC
    GAGAGCCGGG GGTGCTGCTT CATCGAGACC CCCCCTGCAG TGGGGGGCAA GAGGGGGGTG
    CCCTGGTGCT TCTACCCACC CAGCTTCCCC AGCTACGTGG TGCAGAGCCT CAACCAGACG
    GCGCTGGGCA TGACGGGGCT GCTGGTGCGC AGGGAGAAGG CCTATTACCC CAAGGACATC
    CAGGTGCTGA GGATGGACGT GGAGTTCCAG ACGAACACAC GGCTGCGCAT CAAGATAACC
    GATGCAGCCA AGCCCCGGTA TGAGGTGCCC CTGGAAGTTC CCCGAGTGAT GAAGAGAGCA
    GAAAACCCCA TCTACAGCCT GGAGTTCTCC CAGGACCCCT TTGGGGTGCT GCTGAGGCGC
    CAAGGGACAG GGACAGTGCT GCTCAACACC ACCGTGGCTC CCCTGATCTT CGCCGATCAG
    TTCCTGCAGA TCTCCACCAC GCTGCCCTCC AGGTTCCTGT ATGGGCTGGG GGAGCACCGC
    AGCACTCTGC TGCACAGCCT CGACTGGAAC ACCCTCACCC TCTGGGCCCG TGATGTGGCC
    CCCACGGAAT CCTTCAACCT GTACGGCGCT CACCCCTTCT ACCTGCTGAT GGAGGAGGGC
    GGCGATGCCC ACGGGGTTTT CCTCCTCAAC AGCAACGCCA TGGAGGTGGC CCTGCAGCCT
    GCCCCGGGCC TGACTTGGAG GACCATTGGA GGTGTGCTGG ACTTCTACAT CTTCCTGGGG
    CCTGACCCCA ACATGGTCAT CCAGCAGTAC CAGGAGGTGA TAGGTTTCCC AGCCATGCCT
    CCACTCTGGG CGCTCGGCTT CCACCTCTGC CGCTGGGGCT ACGGCTCCAG CAACGAGACC
    TGGCAGACTG CAAGGGCCAT GAGGAACTTC CAGATCCCAC AGGATGCGCA ATGGAACGAC
    ATTGATTACA TGGACGGGTA CCGGGACTTC ACCTTCGATC CCCAGAAATT CGCCTCCCTC
    CCCTCACTGG TGGAAGATCT CCACAAGCAC GGGCAGCACT ACGTGATGAT CTTGGACCCC
    GGCATCAGCA GCACCAGCCC CCACGGCTCC TACTGGCCTT TCGATGAAGG CTTGAGACGT
    GCTTTGTTCC TGAACACCAC CCAGGGGCAG CCACTGATCG GGCAGGTCTG GCCTGGTTAT
    ACTGCCTTCC CCGACTTCTC CAACAGAGAC ACACATCAGT GGTGGCTGGA GAACCTGCAG
    CGCTTCCACA CCCGTGTGCC CTTCGATGGC CTCTGGATCG ACATGAACGA GCCATCCAAC
    TTCATGGATG GGTCTGAAGA GGGCTGCCCA CCCGGAGAGC TCGACAGCCC CCCGTACACG
    CCGGCTGTGC TGGGCAATTC CCTGTCCGCC AAGACGGTGT GTGCCTCAGC GAAGCAGAAC
    GCCTCGGTGC ACTACAACCT GCACAACCTC TACGGGCTGA AGGAAGCCGA AGCGACGGCG
    AGCGCCTTGA TCCACATCCG AGGAAAGCGT CCCTTTGTCA TCTCCCGCTC TACCTTCCCC
    AGCCAGGGCC GGTACTCGGG TCACTGGCTG GGTGACAACC GGAGCCAGTG GAAGGACATG
    TATTACTCCA TCCCAGGGAT GCTGAGCTTC AGCCTGTTTG GGATCCCACT GGTGGGGGCC
    GACATCTGCG GCTTCTCCGG CAGCACCTCG GAGGAGCTGT GCACCCGCTG GATGCAGCTC
    GGGGCCTTCT ACCCCTTCTC CCGCAACCAC AACACCCAGA ACGAGAAGGC CCAGGACCCC
    ACAGCTTTCA GCCCCTCGGC GAGGACAGCC ATGAAGGACG TACTGCTCAC CCGCTACTCC
    CTCCTGCCTT TCCTCTACAC ACTTTTCCAC CGTGCCCACC TGCAAGGGGA AACCGTTGCC
    CGACCTTTGT TCTTTGAGTT CCCCTGGGAT GTGGCCACGT ATGGGCTGGA CAGGCAGTTC
    CTGTGGGGCC AGAGCCTGCT GGTGACACCG GTGCTGGAGC CCGGAGCAGA CTCGGTGTTG
    GGCTATTTCC CCCGAGGCGT GTGGTACGAC TTCTACACGG GCTCCTCTGT GAACAGCAGT
    GGGGAGATGT TGAAGCTGTC AGCCCCCCTG GACCACCTCA ACCTGCATCT CCGGGAGGGT
    TCTATCCTGC CCACACAGAA ACCCGGGACC ACCAGCAAGG CCACCCGAGG GAACCCGCTG
    CGCCTCATTG TGGCCCTGTC CCCACGTGCC ACTGCCTGGG GGGACCTCTT CTGGGATGAC
    GGCGAGAGCC TGGACACCTT CGAGCGGGGC AACTACTCCT ACCTGGTGTT CAATGCCACA
    GAGAACATCT TCACCTCCAA CGTCCTCCAT GCCAGCACCG AGGCCACCTA TGTCACCATC
    GACACGGTGA GCTTCTACGG AATGCAGGAG CCGCCCAGCA AGGTCCTCCT GGATGGGCAG
    GAGAAGCCCT TCTCCTACCT GGACAACCAG GTCCTCACGG TCAGCGGCCT CGGCCTCAGG
    CTCAGCCAGG GCTTCTCCCT GCAGTGGCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.