AvBD3 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	AvBD3
Entrez Gene ID	395363
Full Name	avian beta-defensin 3
Synonyms	DEFB1,GAL 3,GAL3,gal-3
General protein information	Preferred Names avian beta-defensin 3 Names gallinacin-3 antimicrobial peptide-3 beta-defensin 3 antimicrobial peptide beta-defensin prepropeptide defensin, beta 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204650.2	NP_989981.1	gallinacin-3 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Avian host defense peptides.
Cuperus T, Coorens M, van Dijk A, Haagsman HP
Developmental and comparative immunology41(3)352-69(2013 Nov)

Induction of avian ?-defensins by CpG oligodeoxynucleotides and proinflammatory cytokines in hen vaginal cells in vitro.
Sonoda Y, Abdel Mageed AM, Isobe N, Yoshimura Y
Reproduction (Cambridge, England)145(6)621-31(2013 Jun)

The potent adjuvant effects of chicken beta-defensin-1 when genetically fused with infectious bursal disease virus VP2 gene.
Zhang HH, Yang XM, Xie QM, Ma JY, Luo YN, Cao YC, Chen F, Bi YZ
Veterinary immunology and immunopathology136(1-2)92-7(2010 Jul)

Primary structure and antibacterial activity of chicken bone marrow-derived beta-defensins.
Derache C, Labas V, Aucagne V, Meudal H, Landon C, Delmas AF, Magallon T, Lalmanach AC
Antimicrobial agents and chemotherapy53(11)4647-55(2009 Nov)

Differential modulation of beta-defensin gene expression by Salmonella Enteritidis in intestinal epithelial cells from resistant and susceptible chicken inbred lines.
Derache C, Esnault E, Bonsergent C, Le Vern Y, Qu?r? P, Lalmanach AC
Developmental and comparative immunology33(9)959-66(2009 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that DEFB1 and DEFB3 are involved in mechanisms by which the vaginal mucosa forms a more efficient defense system against bacterial infection; proinflammatory cytokines induce DEFB1 and DEFB3 in vagina mucosa cells.
Title: Induction of avian ?-defensins by CpG oligodeoxynucleotides and proinflammatory cytokines in hen vaginal cells in vitro.

potent adjuvant effects of beta-defensin-1 when genetically fused with infectious bursal disease virus VP2 gene
Title: The potent adjuvant effects of chicken beta-defensin-1 when genetically fused with infectious bursal disease virus VP2 gene.

intestinal epithelium express beta-defensin antimicrobial peptides that may play a role in immunoprotection against Salmonella Enteritidis.
Title: Differential modulation of beta-defensin gene expression by Salmonella Enteritidis in intestinal epithelial cells from resistant and susceptible chicken inbred lines.

AvBD-3 is synthesized by late stage of spermatids in the testis, and this molecule is retained by the sperm during the passage of male reproductive tract, and even in the sperm storage tubules of oviduct.
Title: Expression of avian beta-defensin 3, an antimicrobial peptide, by sperm in the male reproductive organs and oviduct in chickens: an immunohistochemical study.

mRNA expression of Gal-1, -2 and -3 in the vagina of laying hens increases with age but decreases in the regressed oviduct during the non-laying phase, and may increase in response to Salmonella enteritidis (SE) and lipopolysaccharide
Title: Effects of age, egg-laying activity, and Salmonella-inoculation on the expressions of gallinacin mRNA in the vagina of the hen oviduct.

The following AvBD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AvBD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa00117	NM_204650.2 Latest version!	Gallus gallus avian beta-defensin 3 (AvBD3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AvBD3 ( NM_204650.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989981.1 Gallus gallus avian beta-defensin 3 (AvBD3), mRNA.

Price & Availability↑

CloneID	OGa00117
Clone ID Related Accession (Same CDS sequence)	NM_204650.2
Accession Version	NM_204650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Gallus gallus(chicken)
Product	gallinacin-3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF181952.1. On Aug 31, 2012 this sequence version replaced NM_204650.1. ##Evidence-Data-START## Transcript exon combination :: AF181952.1, DQ677634.1 [ECO:0000332] ##Evidence-Data-END##

ATGCGGATCG TGTACCTGCT CATCCCCTTC TTCCTCTTGT TTCTCCAGGG TGCTGCAGGA 
ACTGCCACTC AGTGCAGAAT AAGAGGAGGA TTCTGTCGTG TTGGGAGCTG CCGCTTCCCA 
CACATAGCTA TTGGGAAATG TGCAACATTT ATTTCCTGCT GTGGAAGAGC ATATGAGGTT 
GATGCCCTGA ATTCTGTGAG GACATCGCCG TGGCTTCTCG CTCCTGGAAA CAACCCCCAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989981.1
CDS	3..245
Translation	MRIVYLLIPFFLLFLQGAAGTATQCRIRGGFCRVGSCRFPHIAIGKCATFISCCGRAYEVDALNSVRTSPWLLAPGNNPH

Target ORF information:

RefSeq Version	NM_204650.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus avian beta-defensin 3 (AvBD3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204650.2

ORF Insert Sequence:

ATGCGGATCG TGTACCTGCT CATCCCCTTC TTCCTCTTGT TTCTCCAGGG TGCTGCAGGA 
ACTGCCACTC AGTGCAGAAT AAGAGGAGGA TTCTGTCGTG TTGGGAGCTG CCGCTTCCCA 
CACATAGCTA TTGGGAAATG TGCAACATTT ATTTCCTGCT GTGGAAGAGC ATATGAGGTT 
GATGCCCTGA ATTCTGTGAG GACATCGCCG TGGCTTCTCG CTCCTGGAAA CAACCCCCAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Avian host defense peptides.
Developmental and comparative immunology41(3)352-69(2013 Nov)
Cuperus T,Coorens M,van Dijk A,Haagsman HP

Induction of avian ?-defensins by CpG oligodeoxynucleotides and proinflammatory cytokines in hen vaginal cells in vitro.
Reproduction (Cambridge, England)145(6)621-31(2013 Jun)
Sonoda Y,Abdel Mageed AM,Isobe N,Yoshimura Y

The potent adjuvant effects of chicken beta-defensin-1 when genetically fused with infectious bursal disease virus VP2 gene.
Veterinary immunology and immunopathology136(1-2)92-7(2010 Jul)
Zhang HH,Yang XM,Xie QM,Ma JY,Luo YN,Cao YC,Chen F,Bi YZ

Primary structure and antibacterial activity of chicken bone marrow-derived beta-defensins.
Antimicrobial agents and chemotherapy53(11)4647-55(2009 Nov)
Derache C,Labas V,Aucagne V,Meudal H,Landon C,Delmas AF,Magallon T,Lalmanach AC

Differential modulation of beta-defensin gene expression by Salmonella Enteritidis in intestinal epithelial cells from resistant and susceptible chicken inbred lines.
Developmental and comparative immunology33(9)959-66(2009 Sep)
Derache C,Esnault E,Bonsergent C,Le Vern Y,Qu?r? P,Lalmanach AC