IGF2 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	IGF2
Entrez Gene ID	395097
Full Name	insulin like growth factor 2
Synonyms	IGF-II
General protein information	Preferred Names insulin like growth factor 2 Names insulin-like growth factor II insulin-like growth factor 2 (somatomedin A) insulin-like growth factor 2 (somatomedin A)prepro-IGF-IIprepro-insulin-like growth factor-II prepro-IGF-II prepro-insulin-like growth factor-II
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015286525.1	XP_015142011.1	insulin-like growth factor II isoform X1
NM_001030342.3	NP_001025513.1	insulin-like growth factor II precursor
XM_015286525.2	XP_015142011.1	insulin-like growth factor II isoform X1
NM_001030342.2	NP_001025513.1	insulin-like growth factor II precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	IGF2	NP_001121070.1
Mus musculus (house mouse)	Igf2	NP_034644.2
Pan troglodytes (chimpanzee)	IGF2	XP_001153640.3
Danio rerio (zebrafish)	igf2b	NP_001001815.1
Danio rerio (zebrafish)	igf2a	NP_571508.1
Bos taurus (cattle)	IGF2	NP_776512.2
Rattus norvegicus (Norway rat)	Igf2	NP_113699.2
	LOC721120	XP_002799486.1
Canis lupus familiaris (dog)	IGF2	NP_001182755.1
Xenopus tropicalis (tropical clawed frog)	igf2	NP_001107144.1
Gallus gallus (chicken)	IGF2	NP_001025513.1

Gene Ontology
Bibliography

Related articles in PubMed

miRNA-223 upregulated by MYOD inhibits myoblast proliferation by repressing IGF2 and facilitates myoblast differentiation by inhibiting ZEB1.
Li G, Luo W, Abdalla BA, Ouyang H, Yu J, Hu F, Nie Q, Zhang X
Cell death & disease8(10)e3094(2017 Oct)

Developmental changes of Insulin-like growth factors in the liver and muscle of chick embryos.
Liu Y, Guo W, Pu Z, Li X, Lei X, Yao J, Yang X
Poultry science95(6)1396-402(2016 Jun)

Evaluation of the IGFs (IGF1 and IGF2) genes as candidates for growth, body measurement, carcass, and reproduction traits in Beijing You and Silkie chickens.
Tang S, Sun D, Ou J, Zhang Y, Xu G, Zhang Y
Animal biotechnology21(2)104-13(2010 Apr)

Developmental changes of plasma insulin, glucagon, insulin-like growth factors, thyroid hormones, and glucose concentrations in chick embryos and hatched chicks.
Lu JW, McMurtry JP, Coon CN
Poultry science86(4)673-83(2007 Apr)

Potential role for insulin-like growth factor II and vitronectin in the endothelial-mesenchymal transition process.
Arciniegas E, Neves YC, Carrillo LM
Differentiation; research in biological diversity74(6)277-92(2006 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

IGF1 and IGF2 might separately be more important for muscle and liver growth in chick embryonic development. Variation of IGFs expression during the incubation might be concerned with the methylation of gene promoter.
Title: Developmental changes of Insulin-like growth factors in the liver and muscle of chick embryos.

These findings implied that polymorphisms within IGF1 and IGF2 genes could be in linkage disequilibrium with the actual causative mutations that affect growth and carcass traits.
Title: Evaluation of the IGFs (IGF1 and IGF2) genes as candidates for growth, body measurement, carcass, and reproduction traits in Beijing You and Silkie chickens.

Blood levels of IGF-II may be a more important functionary for chick embryonic development than IGF-I.
Title: Developmental changes of plasma insulin, glucagon, insulin-like growth factors, thyroid hormones, and glucose concentrations in chick embryos and hatched chicks.

These findings provide the first evidence for a potential role of the insulin-like growth factor II-vitronectin complex during the endothelial-to-mesenchymal transition process.
Title: Potential role for insulin-like growth factor II and vitronectin in the endothelial-mesenchymal transition process.

These results suggest that the mechanism of action whereby IGFBP2 excess impairs long bone development is to inhibit IGF-I and IGF-II-mediated proliferation and matrix synthesis.
Title: Role of IGFBP2, IGF-I and IGF-II in regulating long bone growth.

The following IGF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IGF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa00177	NM_001030342.3 Latest version!	Gallus gallus insulin like growth factor 2 (IGF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OGa36253	XM_015286525.2 Latest version!	Gallus gallus insulin like growth factor 2 (IGF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
View Old Accession Versions >>
OGa36253	XM_015286525.1	Gallus gallus insulin-like growth factor 2 (IGF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OGa00177	NM_001030342.2	Gallus gallus insulin like growth factor 2 (IGF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IGF2 ( NM_001030342.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001025513.1 Gallus gallus insulin like growth factor 2 (IGF2), mRNA.

Price & Availability↑

CloneID	OGa00177
Clone ID Related Accession (Same CDS sequence)	NM_001030342.3 , NM_001030342.2
Accession Version	NM_001030342.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Gallus gallus(chicken)
Product	insulin-like growth factor II precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000683.1. On Dec 20, 2018 this sequence version replaced NM_001030342.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AY267181.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201358 [ECO:0000348] ##Evidence-Data-END##

ATGTGTGCTG CCAGGCAGAT ACTGCTGCTA CTGCTGGCCT TCCTGGCCTA TGCGTTGGAT 
TCAGCTGCGG CGTATGGCAC GGCGGAGACC CTCTGCGGTG GGGAGCTGGT GGACACACTG 
CAGTTCGTCT GTGGGGACAG GGGCTTCTAC TTCAGTAGAC CAGTGGGACG AAATAACAGG 
AGGATCAACC GTGGCATTGT GGAGGAGTGC TGCTTTCGGA GCTGTGACCT GGCTCTGCTG 
GAAACCTACT GTGCCAAGTC CGTCAAGTCA GAGCGTGACC TCTCCGCCAC CTCCCTCGCG 
GGCCTCCCAG CCCTCAACAA GGAGAGCTTC CAGAAGCCAT CTCATGCCAA GTACTCCAAG 
TACAACGTGT GGCAGAAGAA GAGCTCGCAG CGGCTGCAGC GGGAGGTGCC AGGCATCCTG 
CGTGCCCGTC GGTACCGGTG GCAGGCGGAG GGGCTGCAAG CAGCTGAGGA AGCCAGGGCG 
ATGCATCGTC CCCTCATCTC CTTGCCCAGT CAGCGGCCCC CAGCGCCGCG GGCATCCCCT 
GAAGCGACCG GCCCCCAGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025513.1
CDS	1..564
Translation	MCAARQILLLLLAFLAYALDSAAAYGTAETLCGGELVDTLQFVCGDRGFYFSRPVGRNNRRINRGIVEECCFRSCDLALLETYCAKSVKSERDLSATSLAGLPALNKESFQKPSHAKYSKYNVWQKKSSQRLQREVPGILRARRYRWQAEGLQAAEEARAMHRPLISLPSQRPPAPRASPEATGPQE

Target ORF information:

RefSeq Version	NM_001030342.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus insulin like growth factor 2 (IGF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030342.3

ORF Insert Sequence:

ATGTGTGCTG CCAGGCAGAT ACTGCTGCTA CTGCTGGCCT TCCTGGCCTA TGCGTTGGAT 
TCAGCTGCGG CGTATGGCAC GGCGGAGACC CTCTGCGGTG GGGAGCTGGT GGACACACTG 
CAGTTCGTCT GTGGGGACAG GGGCTTCTAC TTCAGTAGAC CAGTGGGACG AAATAACAGG 
AGGATCAACC GTGGCATTGT GGAGGAGTGC TGCTTTCGGA GCTGTGACCT GGCTCTGCTG 
GAAACCTACT GTGCCAAGTC CGTCAAGTCA GAGCGTGACC TCTCCGCCAC CTCCCTCGCG 
GGCCTCCCAG CCCTCAACAA GGAGAGCTTC CAGAAGCCAT CTCATGCCAA GTACTCCAAG 
TACAACGTGT GGCAGAAGAA GAGCTCGCAG CGGCTGCAGC GGGAGGTGCC AGGCATCCTG 
CGTGCCCGTC GGTACCGGTG GCAGGCGGAG GGGCTGCAAG CAGCTGAGGA AGCCAGGGCG 
ATGCATCGTC CCCTCATCTC CTTGCCCAGT CAGCGGCCCC CAGCGCCGCG GGCATCCCCT 
GAAGCGACCG GCCCCCAGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IGF2 ( NM_001030342.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001025513.1 Gallus gallus insulin like growth factor 2 (IGF2), mRNA.

Price & Availability↑

CloneID	OGa00177
Clone ID Related Accession (Same CDS sequence)	NM_001030342.3 , NM_001030342.2
Accession Version	NM_001030342.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-02
Organism	Gallus gallus(chicken)
Product	insulin-like growth factor II precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN04000238.1. On Feb 11, 2016 this sequence version replaced NM_001030342.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AY267181.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201358 [ECO:0000348] ##Evidence-Data-END##

ATGTGTGCTG CCAGGCAGAT ACTGCTGCTA CTGCTGGCCT TCCTGGCCTA TGCGTTGGAT 
TCAGCTGCGG CGTATGGCAC GGCGGAGACC CTCTGCGGTG GGGAGCTGGT GGACACACTG 
CAGTTCGTCT GTGGGGACAG GGGCTTCTAC TTCAGTAGAC CAGTGGGACG AAATAACAGG 
AGGATCAACC GTGGCATTGT GGAGGAGTGC TGCTTTCGGA GCTGTGACCT GGCTCTGCTG 
GAAACCTACT GTGCCAAGTC CGTCAAGTCA GAGCGTGACC TCTCCGCCAC CTCCCTCGCG 
GGCCTCCCAG CCCTCAACAA GGAGAGCTTC CAGAAGCCAT CTCATGCCAA GTACTCCAAG 
TACAACGTGT GGCAGAAGAA GAGCTCGCAG CGGCTGCAGC GGGAGGTGCC AGGCATCCTG 
CGTGCCCGTC GGTACCGGTG GCAGGCGGAG GGGCTGCAAG CAGCTGAGGA AGCCAGGGCG 
ATGCATCGTC CCCTCATCTC CTTGCCCAGT CAGCGGCCCC CAGCGCCGCG GGCATCCCCT 
GAAGCGACCG GCCCCCAGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025513.1
CDS	1..564
Translation	MCAARQILLLLLAFLAYALDSAAAYGTAETLCGGELVDTLQFVCGDRGFYFSRPVGRNNRRINRGIVEECCFRSCDLALLETYCAKSVKSERDLSATSLAGLPALNKESFQKPSHAKYSKYNVWQKKSSQRLQREVPGILRARRYRWQAEGLQAAEEARAMHRPLISLPSQRPPAPRASPEATGPQE

Target ORF information:

RefSeq Version	NM_001030342.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus insulin like growth factor 2 (IGF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030342.2

ORF Insert Sequence:

ATGTGTGCTG CCAGGCAGAT ACTGCTGCTA CTGCTGGCCT TCCTGGCCTA TGCGTTGGAT 
TCAGCTGCGG CGTATGGCAC GGCGGAGACC CTCTGCGGTG GGGAGCTGGT GGACACACTG 
CAGTTCGTCT GTGGGGACAG GGGCTTCTAC TTCAGTAGAC CAGTGGGACG AAATAACAGG 
AGGATCAACC GTGGCATTGT GGAGGAGTGC TGCTTTCGGA GCTGTGACCT GGCTCTGCTG 
GAAACCTACT GTGCCAAGTC CGTCAAGTCA GAGCGTGACC TCTCCGCCAC CTCCCTCGCG 
GGCCTCCCAG CCCTCAACAA GGAGAGCTTC CAGAAGCCAT CTCATGCCAA GTACTCCAAG 
TACAACGTGT GGCAGAAGAA GAGCTCGCAG CGGCTGCAGC GGGAGGTGCC AGGCATCCTG 
CGTGCCCGTC GGTACCGGTG GCAGGCGGAG GGGCTGCAAG CAGCTGAGGA AGCCAGGGCG 
ATGCATCGTC CCCTCATCTC CTTGCCCAGT CAGCGGCCCC CAGCGCCGCG GGCATCCCCT 
GAAGCGACCG GCCCCCAGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IGF2 ( XM_015286525.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142011.1 Gallus gallus insulin-like growth factor 2 (IGF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa36253
Clone ID Related Accession (Same CDS sequence)	XM_015286525.1 , XM_015286525.2
Accession Version	XM_015286525.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	insulin-like growth factor II isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455879.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCG CCGGGGCACA CACGGATGAG CGCTGCCGGC AGCCTGCCTT CCTCCCCGGC 
CCGCCGCCGA CAGAAGTTGA GAGCGGCAGC GGCAGCGCCA GCGCCAAGGT GCAGAGGATG 
TGTGCTGCCA GGCAGATACT GCTGCTACTG CTGGCCTTCC TGGCCTATGC GTTGGATTCA 
GCTGCGGCGT ATGGCACGGC GGAGACCCTC TGCGGTGGGG AGCTGGTGGA CACACTGCAG 
TTCGTCTGTG GGGACAGGGG CTTCTACTTC AGTAGACCAG TGGGACGAAA TAACAGGAGG 
ATCAACCGTG GCATTGTGGA GGAGTGCTGC TTTCGGAGCT GTGACCTGGC TCTGCTGGAA 
ACCTACTGTG CCAAGTCCGT CAAGTCAGAG CGTGACCTCT CCGCCACCTC CCTCGCGGGC 
CTCCCAGCCC TCAACAAGGA GAGCTTCCAG AAGCCATCTC ATGCCAAGTA CTCCAAGTAC 
AACGTGTGGC AGAAGAAGAG CTCGCAGCGG CTGCAGCGGG AGGTGCCAGG CATCCTGCGT 
GCCCGTCGGT ACCGGTGGCA GGCGGAGGGG CTGCAAGCAG CTGAGGAAGC CAGGGCGATG 
CATCGTCCCC TCATCTCCTT GCCCAGTCAG CGGCCCCCAG CGCCGCGGGC ATCCCCTGAA 
GCGACCGGCC CCCAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142011.1
CDS	633..1313
Translation	MASAGAHTDERCRQPAFLPGPPPTEVESGSGSASAKVQRMCAARQILLLLLAFLAYALDSAAAYGTAETLCGGELVDTLQFVCGDRGFYFSRPVGRNNRRINRGIVEECCFRSCDLALLETYCAKSVKSERDLSATSLAGLPALNKESFQKPSHAKYSKYNVWQKKSSQRLQREVPGILRARRYRWQAEGLQAAEEARAMHRPLISLPSQRPPAPRASPEATGPQE

Target ORF information:

RefSeq Version	XM_015286525.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus insulin-like growth factor 2 (IGF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015286525.1

ORF Insert Sequence:

ATGGCAAGCG CCGGGGCACA CACGGATGAG CGCTGCCGGC AGCCTGCCTT CCTCCCCGGC 
CCGCCGCCGA CAGAAGTTGA GAGCGGCAGC GGCAGCGCCA GCGCCAAGGT GCAGAGGATG 
TGTGCTGCCA GGCAGATACT GCTGCTACTG CTGGCCTTCC TGGCCTATGC GTTGGATTCA 
GCTGCGGCGT ATGGCACGGC GGAGACCCTC TGCGGTGGGG AGCTGGTGGA CACACTGCAG 
TTCGTCTGTG GGGACAGGGG CTTCTACTTC AGTAGACCAG TGGGACGAAA TAACAGGAGG 
ATCAACCGTG GCATTGTGGA GGAGTGCTGC TTTCGGAGCT GTGACCTGGC TCTGCTGGAA 
ACCTACTGTG CCAAGTCCGT CAAGTCAGAG CGTGACCTCT CCGCCACCTC CCTCGCGGGC 
CTCCCAGCCC TCAACAAGGA GAGCTTCCAG AAGCCATCTC ATGCCAAGTA CTCCAAGTAC 
AACGTGTGGC AGAAGAAGAG CTCGCAGCGG CTGCAGCGGG AGGTGCCAGG CATCCTGCGT 
GCCCGTCGGT ACCGGTGGCA GGCGGAGGGG CTGCAAGCAG CTGAGGAAGC CAGGGCGATG 
CATCGTCCCC TCATCTCCTT GCCCAGTCAG CGGCCCCCAG CGCCGCGGGC ATCCCCTGAA 
GCGACCGGCC CCCAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IGF2 ( XM_015286525.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015142011.1 Gallus gallus insulin like growth factor 2 (IGF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa36253
Clone ID Related Accession (Same CDS sequence)	XM_015286525.1 , XM_015286525.2
Accession Version	XM_015286525.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	insulin-like growth factor II isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015286525.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCG CCGGGGCACA CACGGATGAG CGCTGCCGGC AGCCTGCCTT CCTCCCCGGC 
CCGCCGCCGA CAGAAGTTGA GAGCGGCAGC GGCAGCGCCA GCGCCAAGGT GCAGAGGATG 
TGTGCTGCCA GGCAGATACT GCTGCTACTG CTGGCCTTCC TGGCCTATGC GTTGGATTCA 
GCTGCGGCGT ATGGCACGGC GGAGACCCTC TGCGGTGGGG AGCTGGTGGA CACACTGCAG 
TTCGTCTGTG GGGACAGGGG CTTCTACTTC AGTAGACCAG TGGGACGAAA TAACAGGAGG 
ATCAACCGTG GCATTGTGGA GGAGTGCTGC TTTCGGAGCT GTGACCTGGC TCTGCTGGAA 
ACCTACTGTG CCAAGTCCGT CAAGTCAGAG CGTGACCTCT CCGCCACCTC CCTCGCGGGC 
CTCCCAGCCC TCAACAAGGA GAGCTTCCAG AAGCCATCTC ATGCCAAGTA CTCCAAGTAC 
AACGTGTGGC AGAAGAAGAG CTCGCAGCGG CTGCAGCGGG AGGTGCCAGG CATCCTGCGT 
GCCCGTCGGT ACCGGTGGCA GGCGGAGGGG CTGCAAGCAG CTGAGGAAGC CAGGGCGATG 
CATCGTCCCC TCATCTCCTT GCCCAGTCAG CGGCCCCCAG CGCCGCGGGC ATCCCCTGAA 
GCGACCGGCC CCCAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015142011.1
CDS	1641..2321
Translation	MASAGAHTDERCRQPAFLPGPPPTEVESGSGSASAKVQRMCAARQILLLLLAFLAYALDSAAAYGTAETLCGGELVDTLQFVCGDRGFYFSRPVGRNNRRINRGIVEECCFRSCDLALLETYCAKSVKSERDLSATSLAGLPALNKESFQKPSHAKYSKYNVWQKKSSQRLQREVPGILRARRYRWQAEGLQAAEEARAMHRPLISLPSQRPPAPRASPEATGPQE

Target ORF information:

RefSeq Version	XM_015286525.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus insulin like growth factor 2 (IGF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015286525.2

ORF Insert Sequence:

ATGGCAAGCG CCGGGGCACA CACGGATGAG CGCTGCCGGC AGCCTGCCTT CCTCCCCGGC 
CCGCCGCCGA CAGAAGTTGA GAGCGGCAGC GGCAGCGCCA GCGCCAAGGT GCAGAGGATG 
TGTGCTGCCA GGCAGATACT GCTGCTACTG CTGGCCTTCC TGGCCTATGC GTTGGATTCA 
GCTGCGGCGT ATGGCACGGC GGAGACCCTC TGCGGTGGGG AGCTGGTGGA CACACTGCAG 
TTCGTCTGTG GGGACAGGGG CTTCTACTTC AGTAGACCAG TGGGACGAAA TAACAGGAGG 
ATCAACCGTG GCATTGTGGA GGAGTGCTGC TTTCGGAGCT GTGACCTGGC TCTGCTGGAA 
ACCTACTGTG CCAAGTCCGT CAAGTCAGAG CGTGACCTCT CCGCCACCTC CCTCGCGGGC 
CTCCCAGCCC TCAACAAGGA GAGCTTCCAG AAGCCATCTC ATGCCAAGTA CTCCAAGTAC 
AACGTGTGGC AGAAGAAGAG CTCGCAGCGG CTGCAGCGGG AGGTGCCAGG CATCCTGCGT 
GCCCGTCGGT ACCGGTGGCA GGCGGAGGGG CTGCAAGCAG CTGAGGAAGC CAGGGCGATG 
CATCGTCCCC TCATCTCCTT GCCCAGTCAG CGGCCCCCAG CGCCGCGGGC ATCCCCTGAA 
GCGACCGGCC CCCAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miRNA-223 upregulated by MYOD inhibits myoblast proliferation by repressing IGF2 and facilitates myoblast differentiation by inhibiting ZEB1.
Cell death & disease8(10)e3094(2017 Oct)
Li G,Luo W,Abdalla BA,Ouyang H,Yu J,Hu F,Nie Q,Zhang X

Developmental changes of Insulin-like growth factors in the liver and muscle of chick embryos.
Poultry science95(6)1396-402(2016 Jun)
Liu Y,Guo W,Pu Z,Li X,Lei X,Yao J,Yang X

Evaluation of the IGFs (IGF1 and IGF2) genes as candidates for growth, body measurement, carcass, and reproduction traits in Beijing You and Silkie chickens.
Animal biotechnology21(2)104-13(2010 Apr)
Tang S,Sun D,Ou J,Zhang Y,Xu G,Zhang Y

Developmental changes of plasma insulin, glucagon, insulin-like growth factors, thyroid hormones, and glucose concentrations in chick embryos and hatched chicks.
Poultry science86(4)673-83(2007 Apr)
Lu JW,McMurtry JP,Coon CN

Potential role for insulin-like growth factor II and vitronectin in the endothelial-mesenchymal transition process.
Differentiation; research in biological diversity74(6)277-92(2006 Jul)
Arciniegas E,Neves YC,Carrillo LM

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IGF2 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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