ASCL1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASCL1
Entrez Gene ID	386573
Full Name	achaete-scute family bHLH transcription factor 1
Synonyms	CASH1
General protein information	Preferred Names achaete-scute family bHLH transcription factor 1 Names achaete-scute homolog 1 achaete-scute complex homolog 1 achaete-scute complex-like 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204412.1	NP_989743.1	achaete-scute homolog 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Interaction of MTG family proteins with NEUROG2 and ASCL1 in the developing nervous system.
Aaker JD, Patineau AL, Yang HJ, Ewart DT, Nakagawa Y, McLoon SC, Koyano-Nakagawa N
Neuroscience letters474(1)46-51(2010 Apr)

Reprogramming chick RPE progeny cells to differentiate towards retinal neurons by ash1.
Mao W, Yan RT, Wang SZ
Molecular vision142309-20(2008)

A BMP-mediated transcriptional cascade involving Cash1 and Tlx-3 specifies first-order relay sensory neurons in the developing hindbrain.
Hornbruch A, Ma G, Ballermann MA, Tumova K, Liu D, Cairine Logan C
Mechanisms of development122(7-8)900-13(2005 Jul)

Interaction of Mash1 and Phox2b in sympathetic neuron development.
Stanke M, Stubbusch J, Rohrer H
Molecular and cellular neurosciences25(3)374-82(2004 Mar)

A chicken achaete-scute homolog (CASH-1) is expressed in a temporally and spatially discrete manner in the developing nervous system.
Jasoni CL, Walker MB, Morris MD, Reh TA
Development (Cambridge, England)120(4)769-83(1994 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

There is a functional relationship between specific MTG and bHLH proteins in developing chick spinal cord.
Title: Interaction of MTG family proteins with NEUROG2 and ASCL1 in the developing nervous system.

This study explores the possibility of reprogramming retinal pigment epithelium progeny cells to differentiate toward retinal neurons with achaete-scute homolog 1 (ash1), a proneural gene that is expressed in progenitor cells in the developing retina.
Title: Reprogramming chick RPE progeny cells to differentiate towards retinal neurons by ash1.

a BMP-mediated transcriptional cascade involving Cash1 and Tlx-3 specifies first-order relay sensory neurons in the developing brainstem
Title: A BMP-mediated transcriptional cascade involving Cash1 and Tlx-3 specifies first-order relay sensory neurons in the developing hindbrain.

induces neuronal differentiation characterized by the expression of generic neuronal genes SCG10, Hu and NF160
Title: Interaction of Mash1 and Phox2b in sympathetic neuron development.

The following ASCL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASCL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa00419	NM_204412.1 Latest version!	Gallus gallus achaete-scute family bHLH transcription factor 1 (ASCL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASCL1 ( NM_204412.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989743.1 Gallus gallus achaete-scute family bHLH transcription factor 1 (ASCL1), mRNA.

Price & Availability↑

CloneID	OGa00419
Clone ID Related Accession (Same CDS sequence)	NM_204412.1
Accession Version	NM_204412.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	achaete-scute homolog 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U01339.1. ##Evidence-Data-START## Transcript exon combination :: U01339.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCCAGCG GCAGCCCCGC CGGGATGGCC AGCGGGCAGC CGCCCTTCCT GCAGCCCGCC 
TGCTTCTTCG CCGCCGCCGT GGCCGCCGCC GCCGCCGCCG CTCCGCCGGG GCCGCCCCCG 
GGGGCGCCGC CGCCGCCGCT CCCGCAGCTG AGCCCGGCGG GCGGGCGGCC GTCTCCCGGC 
GGGAAGCCGT CCGCGGCGCG GGCCGCCAAG CGGCAGCGCT CGGCCTCGCC GGAGCTGATG 
CGCTGCAAGA GGCGGCTCAA CTTCAGCGGC TTCGGCTACA GCCTCCCGCA GCAGCAGCCC 
GCCGCCGTAG CGCGGCGCAA CGAGCGGGAG CGCAACCGAG TCAAGCTGGT GAACCTTGGG 
TTCGCCACGC TCCGCGAGCA CGTCCCCAAC GGCGCGGCTA ACAAGAAGAT GAGCAAAGTG 
GAGACGCTGC GCTCCGCCGT CGAGTACATC CGCGCCCTGC AGCAGCTGCT CGACGAGCAC 
GACGCCGTCA GCGCCGCCTC CCAGGCCGGC GTGCTGTCGC CCACCATCTC GCCCGGTTAC 
TCCCACGACA TGAACTCCAT GGCGGGCTCC CCCGTCTCCT CCTACTCCTC CGACGAGGGC 
TCCTACGACC CGCTCAGCCC CGAGGAGCAG GAGCTGCTCG ACTTCACCAG CTGGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989743.1
CDS	255..914
Translation	MASGSPAGMASGQPPFLQPACFFAAAVAAAAAAAPPGPPPGAPPPPLPQLSPAGGRPSPGGKPSAARAAKRQRSASPELMRCKRRLNFSGFGYSLPQQQPAAVARRNERERNRVKLVNLGFATLREHVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAVSAASQAGVLSPTISPGYSHDMNSMAGSPVSSYSSDEGSYDPLSPEEQELLDFTSWF

Target ORF information:

RefSeq Version	NM_204412.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus achaete-scute family bHLH transcription factor 1 (ASCL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204412.1

ORF Insert Sequence:

ATGGCCAGCG GCAGCCCCGC CGGGATGGCC AGCGGGCAGC CGCCCTTCCT GCAGCCCGCC 
TGCTTCTTCG CCGCCGCCGT GGCCGCCGCC GCCGCCGCCG CTCCGCCGGG GCCGCCCCCG 
GGGGCGCCGC CGCCGCCGCT CCCGCAGCTG AGCCCGGCGG GCGGGCGGCC GTCTCCCGGC 
GGGAAGCCGT CCGCGGCGCG GGCCGCCAAG CGGCAGCGCT CGGCCTCGCC GGAGCTGATG 
CGCTGCAAGA GGCGGCTCAA CTTCAGCGGC TTCGGCTACA GCCTCCCGCA GCAGCAGCCC 
GCCGCCGTAG CGCGGCGCAA CGAGCGGGAG CGCAACCGAG TCAAGCTGGT GAACCTTGGG 
TTCGCCACGC TCCGCGAGCA CGTCCCCAAC GGCGCGGCTA ACAAGAAGAT GAGCAAAGTG 
GAGACGCTGC GCTCCGCCGT CGAGTACATC CGCGCCCTGC AGCAGCTGCT CGACGAGCAC 
GACGCCGTCA GCGCCGCCTC CCAGGCCGGC GTGCTGTCGC CCACCATCTC GCCCGGTTAC 
TCCCACGACA TGAACTCCAT GGCGGGCTCC CCCGTCTCCT CCTACTCCTC CGACGAGGGC 
TCCTACGACC CGCTCAGCCC CGAGGAGCAG GAGCTGCTCG ACTTCACCAG CTGGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interaction of MTG family proteins with NEUROG2 and ASCL1 in the developing nervous system.
Neuroscience letters474(1)46-51(2010 Apr)
Aaker JD,Patineau AL,Yang HJ,Ewart DT,Nakagawa Y,McLoon SC,Koyano-Nakagawa N

Reprogramming chick RPE progeny cells to differentiate towards retinal neurons by ash1.
Molecular vision142309-20(2008)
Mao W,Yan RT,Wang SZ

A BMP-mediated transcriptional cascade involving Cash1 and Tlx-3 specifies first-order relay sensory neurons in the developing hindbrain.
Mechanisms of development122(7-8)900-13(2005 Jul)
Hornbruch A,Ma G,Ballermann MA,Tumova K,Liu D,Cairine Logan C

Interaction of Mash1 and Phox2b in sympathetic neuron development.
Molecular and cellular neurosciences25(3)374-82(2004 Mar)
Stanke M,Stubbusch J,Rohrer H

A chicken achaete-scute homolog (CASH-1) is expressed in a temporally and spatially discrete manner in the developing nervous system.
Development (Cambridge, England)120(4)769-83(1994 Apr)
Jasoni CL,Walker MB,Morris MD,Reh TA

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ASCL1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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