fs(1)N cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	fs(1)N
Entrez Gene ID	45973
Full Name	female sterile (1) Nasrat
Synonyms	371,8D8.1,CG11411,Dmel\CG11411,EG:8D8.1,Fs(1)N,Nas,fs(1)A371,fs(1)K1540,fs(1)M6,fs(1)Nas,fs(1)Nasrat,fs(1)[nas],nas
General protein information	Preferred Names female sterile (1) Nasrat Names CG11411 gene product from transcript CG11411-RA CG11411-PA female sterile (1) nasrat fs(1)N-PA fs(1)Nasrat nasrat
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 1F3-1F3\|1-0.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_130545.3	NP_569901.1	female sterile (1) nasrat

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

move from the oocyte to the follicle cells in process that does not require endocytosis
Title: Germline and somatic vitelline proteins colocalize in aggregates in the follicular epithelium of Drosophila ovaries.

closca is a gene encoding a new member of a group of proteins that act non-redundantly in vitelline membrane biogenesis and in Torso signalling; Nasrat protein is another member of this group incorporated into the vitelline membrane
Title: closca, a new gene required for both Torso RTK activation and vitelline membrane integrity. Germline proteins contribute to Drosophila eggshell composition.

Nasrat and Polehole are essential for extracellular accumulation of Torso-like, a factor secreted during oogenesis that initiates Torso receptor activation
Title: Cell surface proteins Nasrat and Polehole stabilize the Torso-like extracellular determinant in Drosophila oogenesis.

Required for cross-linkage of the vitelline membrane.
Title: Drosophila vitelline membrane cross-linking requires the fs(1)Nasrat, fs(1)polehole and chorion genes activities.

The following fs(1)N gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fs(1)N cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02398	NM_130545.3 Latest version!	Drosophila melanogaster female sterile (1) nasrat (fs(1)N), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fs(1)N ( NM_130545.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569901.1 Drosophila melanogaster female sterile (1) nasrat (fs(1)N), mRNA.

Price & Availability↑

CloneID	OFa02398
Clone ID Related Accession (Same CDS sequence)	NM_130545.3
Accession Version	NM_130545.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	female sterile (1) nasrat
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_130545.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTAATTT GGCACCTGCT GCTCTGGCCA GCATTAATAG CCAGTGCCCC ACCGCGGCCC 
CTGACCGACC AAAAGGCCGA GGTGGCCAAG CTGCGAAGCG AACTAGAAAA AGCTCTGGAA 
CACGACGATG GAGGAGCACC ACCAGGAGTG GTGGTGGCAC CCACGTGGAC CCAGCAGGCG 
GGGCAAATGC CGCTTGGAAA GACCTCCAAA GTGGTGGTGG ACCATAGCTC GCTGAGCAGC 
GCCTCCGCCA TTCGCCAGGA GATTCAGCAG GCGGTGGCTC ACCAGGGACA GAACCAAGAG 
GTCCTCCAGC TGGAAGCTCT GCTGAAAGCA AGAGGCAGCA GCAAGGAGAG CACGGCTCAA 
GTGTCCACGT CCCGTCAGGA GGTGCTGGGA GCAACTGTAC AGGAAGCCAA TGTGGTGGAG 
TCGCACTCGA CATCGCAGAC CGTGGACCTG GAACAACTAC AGCGCCTGGA GCTGTCCAAG 
AGGCGCACCC GCGACATGCT CGTCCTGGGT CGCATTGAAG AGTTGCTCAA CTACAGCATG 
CCGACGGATG TGGATCGCTG GCTGACCCTC CAGGCCAATG GAAGCGTTTT CCTTGTGGGT 
CAGCAGCAGG AGGGCCTCCT GGTTCTTTCA GAGGAATTCA CTCCGTTGCA GTCTTATGTC 
CACCCCTTTC CCATCACCGC CATGCTGGGA ACAGATCGCT GGAACAGAAG GGTGCATATG 
CAGGAAGGTT TGCTGGTGAT GGCTTCCCAG GATCAGCTTA TCTGGCTGCG ATTGGAGCCC 
AGTCTTGGGC TAAAACCCAT CTGGCACTGG ACGATTGGCT CGCATGTGAC CAAGATTAGA 
GCATTCAACA TGGAGGGACA AGATTACCTC GCTCTCGTAG CAAATCACAC GCTTAATATC 
TACGTCTATG ATTTGGAAGC GGAGGAGTTC TGGATCGCAC AACGTGTGCA AATGGCAGAG 
ACGATCTCCA ACCTGGCCAT TCTCGATACA GGGAGGGAAC TGCTCTTGGC AGTGGGTCAG 
TGGGACGAGG TTCTGATATA CGCCTGCACT TCGAAGGGGC AACCGCTGCA CTTGCGCCAA 
CAAGTTGCTG CCCCCGAGGT GGCCGGCGTC ACTGCCTTCC AAATGGGCGG ACGCAGCTAC 
CTTGCGCTGG GTGGAATAAT GCCGCAGATC CTGGTCTACG TGCAGGGTCA GCTTGTGCCA 
CGCACCATTC TTGGCCAGAA CTTTGGATTT GTAGACCACT ACCTTCCAAT CCCCGTGCGC 
AGCTACAGAG ACGACCTACT TCTGCTCGTC CAGCATCGAG TTGTCTTTGA CACACATTCA 
CTGCTCGTCC TGGAGGTTTT GGTTTGGAAC GGTGAGGCGT TTGAGGCGGG CTTGCCGCCT 
CCTTGTGGTA CTGCCTATGG GGCGGGCTGC ATGTTGGATC AGGATCGTGA GGCTGGCATT 
TCAGGAGCTG TCCTGCTCCG CCGCTCGACG AATCAGCCGC CGCTGGTTGT GGTGCCCAGG 
AAAGAAGCTC CTACGGGCAT TTTTGTGCTG GAAACTCAAT TGCTGGCTAG GAATTCGGAG 
ACACAGGACC TGCTGGAGAT CCGACAGTTC ATGCAGGATT GGGTGCACGA ACAGGAAGAG 
CTAATCCAGC TGGCTGAAGA GCTCTTGGCA GAGAAGTCGG AGAGTCAGTA CATCGAGGAG 
CTATCAACTC CACTGGTTGT AAGCGAGGGC GGAACCGTTG AGGAACTTTT CGTTAATGAA 
GCTCGCTGGA CGGGAGCGGA TGCTGTTGTG GATGTAAGCT CCCTGCTTCA GCAGATACTT 
CTGTTGGACG GGGAAATGAG TTCTAGGCGA TCTAAACGCC AGCCAGAGAC GCTGTTCAAC 
TTTCACTACG AGCAACTGGA AGTAGAAGCC GTTGACTCTG TGGAACTTAA CTTGGAGAAG 
CTCAACCATG AGACGTTCTA TATCCGAAAT GGCTCCCTTG ACTTGCATCT GGGAACCCTC 
AATGTTCAGC AATTGGAACT TCTGGAAGCT CCGAAATTGG AGCTCGTCCC AGTCCACGGT 
GCAGAATCAT ACAAAATTCT GAAGATGGCT ACGGATTTGG ACAGCATTTT TATTAACGAA 
ATGGAGTGGG ACAAGCTGCT TCAAGATCTG GTGTGGCGTC ATCAGCCTCT TAAGCTTTCG 
CAGTTACATG TAGAGGGACC GGTCATTTTC GAAGATGTAC TAAGTTTGCA CTCTCTGAAC 
GACCTAAGCT TTCCAGGAGA CTATCTCTGG TCGCAGGGAA ATGAGACGAG TGTTGTCCAG 
GCGCCAAAGG AGTTTACGCA AACGCTTTCT GCAAATGCTG TGGACACGAC GGGAACCATC 
AACGCCGTAT ACCCGCATGA TGTCATTACT CTCAGTGATT CCCAGGAATG GCCGGGATTG 
GTAACCTTCT CGCATTTGGA GGTTTCTGAA GAATTGGAGC TCAATGGCAG CGCCCAGGGA 
AGGCAGTTTG AGGAGGCACC TTTAAATCCC ACTCTGCTGG AGTCACGTGT TATCAGTGCC 
GACTGCCATT TCGAACAGCT TCTTGTGCGT GGCCCTTTGC GGCTGCTTGG AAAGGTGGAT 
AACGACAGCT TTGGCTCTCT GCTAGGAGAT CTCGTTCAGC GTTCGCCGAA CTCTGCCCAA 
GAACTACAGA TAAGTGGACA AAAGCGAGTT GACCAGCTGC TTCTGCCAGT GGATGCTCAT 
ATCGCAGATA ATCAGCTCAG TGGAATTTTC CTCAATGACT TTGTTACCAA GCACACGCCA 
CAAACGCTGT CGAATCTGAG TCAACTGGGA GGGTATGTTT ACTTTCATCA GCTGGAGCTT 
GGGAAAGAGT CCTCTTACGA CGGAGTTCGC CTAGAGGAAC TACTCTCCAA AAGTCTTCGA 
CTAGATAGCC CATTTCCCCT GAGTTCTCCC CACACACGCC TTCGGTTTGT TGGACATGCC 
CCAAATTTCA CCCGGCTACA AGTTGAAAAC ACCTTGAACA AGGTGCCGCT GTCAAGTGGC 
TACCAGCTGC TCCATGAACC GCTCCATCTA GGCAGAGCTA ACTTTAGTCG TCTGATGGCA 
GGGCAAGCGC AGGTTAACTA TGATGTCTCT GGCAACGGGC TACTCAATGG ACGAAGCCTG 
ATCCGTATTC TACAGGAGCA ACCTAGGACT TGGTCGGGTG AAGTGCACGT GCAAGAGCTT 
ATTCTTCCCC AGGGTGTGCA GGCGAACCAG CTGCAGGGCA TTAAGGCAGA CCTTCTGCTG 
GATTTTCTGC AGCAGCTGGA CGAGCTCCCG TTGCTGATCC TTCAGGGTCG CATCCAAGTT 
GAACGCATCG CCGTCAACGG TTCGGTGCAA GCAGCTGAAA CCTTGAACAT GCAACCCTTC 
CACGAACTTC AGAGGCAAGT CGTTTGGCTG GATAGGCAGA ACGAATTAGG GACGCGTTGG 
GTTCTGAAAG AATCTCCCCA GTTCCAGCAG AACCTCCAAG TTCTGGGCAG CTTTAACGAG 
CGCCTGCTCC CAGAATTGCT GGATGACATT GTGCTGCGCT CCGACGCGCA CGAGGTGCTG 
ATCGAGGGCA CCAAAAGCTT CTTGGCTCCT GTTAACACTA AAGAGCTTCA TCTGGCTGCA 
CTGAATGGGA TTCCCTTCAA TCACATGGCC AACAAATTAT CTCCGCTGCA CCTCACTGGA 
AATGTCCGCA TTCAGGGACG CATCTTTGTG GAGGACCTGC AGCTAAATGG TCAAGTAAAC 
GGAGATCCGG ACATTCCTGC TGATCTGCAG AAACTGCTCC GCTGGGATCC AAATGCAGGT 
GGCTTTGTGC ACCGGGGAGT CGTTGAACTT CCAAAAAAGG AGCTAGAACG CCTAGTGGTT 
TTGGGCCACT TGAAAAATCG CAGCCGTGAG CCCGTTCAGG AACTCTTTGA TCAGCTAGTC 
TTCAAGCAGC AGCGTGGCAT ACACTTGCAT GGTCACAAGA CTTTCACAGG CCGTCTTCGC 
ATTGAAGATG GAGCTCACAT CAAGTTCTTC AATGGACGCA ACCTAGAACA GTTCCTAGAG 
AATTTAATCT ACGTGGATTC GCAGGGAGAG ATACGTTTGG AAACACCTGT TCGCTTTGCG 
GCTGATGTTA AAATGGATCA TCTTGAAGCA GATCGCCTTG TACTGTCGGG AGAGCTGCTT 
AACGGATGCA ATGTGAGCCA GTGGCTCCGG GACACTATTC GTGTCGATCG CGATTTCCAA 
CAGCCTGAGA TTGCGTTTGC AAAGGGATCG CTGGATGGTA ATTTCTTGGA GGTTGAGGAG 
CTTAACGAAG TTAATCTCTC ACTGGTTGTC ACCCGCTTCA CGGAGCAACA GCTAAGCGAT 
CCCCTAGAGG CAGATGATTT GCTCCTAGAT GGCCAGCTGG TGGTAAGGGG CACTGTTAAT 
GGTTACAATC TACCAGAGGA GTATGCCAAC ACTTTAATGA TTAACCCATT GCGAGAGCAG 
CAAGTGGATA CGCCTTTATT GTTGTCTAAC ATTTTTGTCA CCGGACCCCT GGAGGTAACT 
GCTCCAGTTC ATGGTCTAAA CATAAGCGAT GTGGCAATTT TGGGCGAAGA TCCAGTGCTG 
CTGCAATCGC CGCTATATTT CGACACTCTG CATGCTCCGT TTTTGAGAGC GCAACAGAAC 
ATCAATGGAT TTGACTTTAG GGCTTGGTAC GGAGGAAGCT TATGGGCAAG AGGTCGGGAG 
CAGCAGGAAA TAAGCGGGAA CTGGCGCGTA AAGAAACTGC AGGTGAAGCA GGGTGATGAG 
CTGCGACCAC GTCGACAAAC TGCAGAAGAG AGCTACCGGG AGTTCTGCGA GGGTTTGGTC 
AGGATTCTCC TGCCTTACAA GGTCCAGAAG CTGCGGCGGA GATTTGATTT AAATCAGGAA 
AGGGATCAGG AGAATATTCG CCGAGTATTT GCTTTGGAGG CACCCGGGGG CATTAGTTAT 
CTTCTGATCA ACGAGCGAGG TTGCTGGACC CGCATCCACC GCTGGAATGG ATCTTTATTC 
GATCGATCCG GAGCCTTCAA GAGCGGACCT GTGGACGAGG CAGTTGCTTT ACGGGTGGGA 
AACACAAGCT CTAACCAGGA CTTTGCCTTT ATGACCAGCT ACGAGTTGCA GGATGATCAG 
AACGAGCCAA GCTTGAATTG CAGTGCAGTG AAGCCCATTC TGCTGAGCTG GAACCTGAAC 
AAAACAAAGA GCACTACTGA GCACATGGAA CTATCAGCGG ACACCCTAAG AAATCTCAAA 
GATCAATATG AGCAGCTTAA AAACCAGCCA CTCCCTAATC CCAGTTACCA GCAAGCTTTT 
AAGTATCTGC AGCGTCCCAG CATCAAGTCT CAGCTCGGAT CACAGTGGCA AGAGAATAAA 
CATGAACTGA GTCCTTCGGA GATGGCCAGA ATGCGGAGAC GTCTGCTGGA CACCTTGGAA 
TTCCGGTTGC AAGCGGAGGT AAACATTACC CAGTTAAGTA TTCCAGAAAG CGACCTATTC 
GACGAGCACC TTGTGGAGGA TTTTCTGGAG TTGATGCGCC AATTGCGAAC CTTGCGCCGG 
CGTCTAGATA CAGAGACTCT TCCACTGCCA AACACGCCGG CAAGAGTTCT GGCCGCACGG 
AGTGCCCAGC TCATCTGGCC CACGTTGCAG GAGTTGCGAG AGGTTTCGAG TGGAAACGAT 
TCTAGCTTCC AGGAGCAAGT TCTGGAGCAA ACGCTGCTGG ATGTGCTGAT GCTGGCTAAT 
GAAGAAAATG GCTCGGGGGA TGATGAAAAG CTGCATGCGG TGATTCAGAG ATTGAGAAAC 
CTTAAAGGAG AGCTTCAAGG GCAAGAGGAA GACGACGAGG GACCTGCTGC CTCCTATTCG 
GACAGAAATG AGCAGCGTTT GCCGCTACCA GAAGTCGACT GGAGACCCAT TCAAACACTT 
CGCCTCGCTG TGGGACCAGC AAACCGTCCC CGACTTCTTT ACGCCCGGCT GACCGTGGTG 
ACACCAACAG ACGGTCCTCC GCCCACCACG CCCTCCACTG CGCCAGCCGC CCACATCCAA 
CTGCATCATG CAAATGGATC GCTCTTTCAA ACGCTAGCAG GGGAGCATGG AGCACGCCAT 
CTGACATCGT TGCGGGTACG AGATGAGACG CTGCTAGCAT TTATGGAGGG ATGCTGCCGT 
ATAAGAGTGC TTATCTACCG CGGTGTGCAG GGATTCGTGG ACTTTGTCCG TTTCCCCGCA 
CCTGCCCAAG AAGCTAAGAG CGGAGATGGG GAGGTCTTGC AGTTACTATC CCTACGCTTA 
CCTCTCAATC GGCCACCCGG AGCCATGTAC TTATTGGCAG TGGTTCAAGC TCGACGCGTC 
ACTTTCTACG AAGTGGTGGT AGCCGGATTA CTAGAGCCGT GGCTCCAGTG CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569901.1
CDS	62..6418
Translation	MLIWHLLLWPALIASAPPRPLTDQKAEVAKLRSELEKALEHDDGGAPPGVVVAPTWTQQAGQMPLGKTSKVVVDHSSLSSASAIRQEIQQAVAHQGQNQEVLQLEALLKARGSSKESTAQVSTSRQEVLGATVQEANVVESHSTSQTVDLEQLQRLELSKRRTRDMLVLGRIEELLNYSMPTDVDRWLTLQANGSVFLVGQQQEGLLVLSEEFTPLQSYVHPFPITAMLGTDRWNRRVHMQEGLLVMASQDQLIWLRLEPSLGLKPIWHWTIGSHVTKIRAFNMEGQDYLALVANHTLNIYVYDLEAEEFWIAQRVQMAETISNLAILDTGRELLLAVGQWDEVLIYACTSKGQPLHLRQQVAAPEVAGVTAFQMGGRSYLALGGIMPQILVYVQGQLVPRTILGQNFGFVDHYLPIPVRSYRDDLLLLVQHRVVFDTHSLLVLEVLVWNGEAFEAGLPPPCGTAYGAGCMLDQDREAGISGAVLLRRSTNQPPLVVVPRKEAPTGIFVLETQLLARNSETQDLLEIRQFMQDWVHEQEELIQLAEELLAEKSESQYIEELSTPLVVSEGGTVEELFVNEARWTGADAVVDVSSLLQQILLLDGEMSSRRSKRQPETLFNFHYEQLEVEAVDSVELNLEKLNHETFYIRNGSLDLHLGTLNVQQLELLEAPKLELVPVHGAESYKILKMATDLDSIFINEMEWDKLLQDLVWRHQPLKLSQLHVEGPVIFEDVLSLHSLNDLSFPGDYLWSQGNETSVVQAPKEFTQTLSANAVDTTGTINAVYPHDVITLSDSQEWPGLVTFSHLEVSEELELNGSAQGRQFEEAPLNPTLLESRVISADCHFEQLLVRGPLRLLGKVDNDSFGSLLGDLVQRSPNSAQELQISGQKRVDQLLLPVDAHIADNQLSGIFLNDFVTKHTPQTLSNLSQLGGYVYFHQLELGKESSYDGVRLEELLSKSLRLDSPFPLSSPHTRLRFVGHAPNFTRLQVENTLNKVPLSSGYQLLHEPLHLGRANFSRLMAGQAQVNYDVSGNGLLNGRSLIRILQEQPRTWSGEVHVQELILPQGVQANQLQGIKADLLLDFLQQLDELPLLILQGRIQVERIAVNGSVQAAETLNMQPFHELQRQVVWLDRQNELGTRWVLKESPQFQQNLQVLGSFNERLLPELLDDIVLRSDAHEVLIEGTKSFLAPVNTKELHLAALNGIPFNHMANKLSPLHLTGNVRIQGRIFVEDLQLNGQVNGDPDIPADLQKLLRWDPNAGGFVHRGVVELPKKELERLVVLGHLKNRSREPVQELFDQLVFKQQRGIHLHGHKTFTGRLRIEDGAHIKFFNGRNLEQFLENLIYVDSQGEIRLETPVRFAADVKMDHLEADRLVLSGELLNGCNVSQWLRDTIRVDRDFQQPEIAFAKGSLDGNFLEVEELNEVNLSLVVTRFTEQQLSDPLEADDLLLDGQLVVRGTVNGYNLPEEYANTLMINPLREQQVDTPLLLSNIFVTGPLEVTAPVHGLNISDVAILGEDPVLLQSPLYFDTLHAPFLRAQQNINGFDFRAWYGGSLWARGREQQEISGNWRVKKLQVKQGDELRPRRQTAEESYREFCEGLVRILLPYKVQKLRRRFDLNQERDQENIRRVFALEAPGGISYLLINERGCWTRIHRWNGSLFDRSGAFKSGPVDEAVALRVGNTSSNQDFAFMTSYELQDDQNEPSLNCSAVKPILLSWNLNKTKSTTEHMELSADTLRNLKDQYEQLKNQPLPNPSYQQAFKYLQRPSIKSQLGSQWQENKHELSPSEMARMRRRLLDTLEFRLQAEVNITQLSIPESDLFDEHLVEDFLELMRQLRTLRRRLDTETLPLPNTPARVLAARSAQLIWPTLQELREVSSGNDSSFQEQVLEQTLLDVLMLANEENGSGDDEKLHAVIQRLRNLKGELQGQEEDDEGPAASYSDRNEQRLPLPEVDWRPIQTLRLAVGPANRPRLLYARLTVVTPTDGPPPTTPSTAPAAHIQLHHANGSLFQTLAGEHGARHLTSLRVRDETLLAFMEGCCRIRVLIYRGVQGFVDFVRFPAPAQEAKSGDGEVLQLLSLRLPLNRPPGAMYLLAVVQARRVTFYEVVVAGLLEPWLQCP

Target ORF information:

RefSeq Version	NM_130545.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster female sterile (1) nasrat (fs(1)N), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130545.3

ORF Insert Sequence:

ATGCTAATTT GGCACCTGCT GCTCTGGCCA GCATTAATAG CCAGTGCCCC ACCGCGGCCC 
CTGACCGACC AAAAGGCCGA GGTGGCCAAG CTGCGAAGCG AACTAGAAAA AGCTCTGGAA 
CACGACGATG GAGGAGCACC ACCAGGAGTG GTGGTGGCAC CCACGTGGAC CCAGCAGGCG 
GGGCAAATGC CGCTTGGAAA GACCTCCAAA GTGGTGGTGG ACCATAGCTC GCTGAGCAGC 
GCCTCCGCCA TTCGCCAGGA GATTCAGCAG GCGGTGGCTC ACCAGGGACA GAACCAAGAG 
GTCCTCCAGC TGGAAGCTCT GCTGAAAGCA AGAGGCAGCA GCAAGGAGAG CACGGCTCAA 
GTGTCCACGT CCCGTCAGGA GGTGCTGGGA GCAACTGTAC AGGAAGCCAA TGTGGTGGAG 
TCGCACTCGA CATCGCAGAC CGTGGACCTG GAACAACTAC AGCGCCTGGA GCTGTCCAAG 
AGGCGCACCC GCGACATGCT CGTCCTGGGT CGCATTGAAG AGTTGCTCAA CTACAGCATG 
CCGACGGATG TGGATCGCTG GCTGACCCTC CAGGCCAATG GAAGCGTTTT CCTTGTGGGT 
CAGCAGCAGG AGGGCCTCCT GGTTCTTTCA GAGGAATTCA CTCCGTTGCA GTCTTATGTC 
CACCCCTTTC CCATCACCGC CATGCTGGGA ACAGATCGCT GGAACAGAAG GGTGCATATG 
CAGGAAGGTT TGCTGGTGAT GGCTTCCCAG GATCAGCTTA TCTGGCTGCG ATTGGAGCCC 
AGTCTTGGGC TAAAACCCAT CTGGCACTGG ACGATTGGCT CGCATGTGAC CAAGATTAGA 
GCATTCAACA TGGAGGGACA AGATTACCTC GCTCTCGTAG CAAATCACAC GCTTAATATC 
TACGTCTATG ATTTGGAAGC GGAGGAGTTC TGGATCGCAC AACGTGTGCA AATGGCAGAG 
ACGATCTCCA ACCTGGCCAT TCTCGATACA GGGAGGGAAC TGCTCTTGGC AGTGGGTCAG 
TGGGACGAGG TTCTGATATA CGCCTGCACT TCGAAGGGGC AACCGCTGCA CTTGCGCCAA 
CAAGTTGCTG CCCCCGAGGT GGCCGGCGTC ACTGCCTTCC AAATGGGCGG ACGCAGCTAC 
CTTGCGCTGG GTGGAATAAT GCCGCAGATC CTGGTCTACG TGCAGGGTCA GCTTGTGCCA 
CGCACCATTC TTGGCCAGAA CTTTGGATTT GTAGACCACT ACCTTCCAAT CCCCGTGCGC 
AGCTACAGAG ACGACCTACT TCTGCTCGTC CAGCATCGAG TTGTCTTTGA CACACATTCA 
CTGCTCGTCC TGGAGGTTTT GGTTTGGAAC GGTGAGGCGT TTGAGGCGGG CTTGCCGCCT 
CCTTGTGGTA CTGCCTATGG GGCGGGCTGC ATGTTGGATC AGGATCGTGA GGCTGGCATT 
TCAGGAGCTG TCCTGCTCCG CCGCTCGACG AATCAGCCGC CGCTGGTTGT GGTGCCCAGG 
AAAGAAGCTC CTACGGGCAT TTTTGTGCTG GAAACTCAAT TGCTGGCTAG GAATTCGGAG 
ACACAGGACC TGCTGGAGAT CCGACAGTTC ATGCAGGATT GGGTGCACGA ACAGGAAGAG 
CTAATCCAGC TGGCTGAAGA GCTCTTGGCA GAGAAGTCGG AGAGTCAGTA CATCGAGGAG 
CTATCAACTC CACTGGTTGT AAGCGAGGGC GGAACCGTTG AGGAACTTTT CGTTAATGAA 
GCTCGCTGGA CGGGAGCGGA TGCTGTTGTG GATGTAAGCT CCCTGCTTCA GCAGATACTT 
CTGTTGGACG GGGAAATGAG TTCTAGGCGA TCTAAACGCC AGCCAGAGAC GCTGTTCAAC 
TTTCACTACG AGCAACTGGA AGTAGAAGCC GTTGACTCTG TGGAACTTAA CTTGGAGAAG 
CTCAACCATG AGACGTTCTA TATCCGAAAT GGCTCCCTTG ACTTGCATCT GGGAACCCTC 
AATGTTCAGC AATTGGAACT TCTGGAAGCT CCGAAATTGG AGCTCGTCCC AGTCCACGGT 
GCAGAATCAT ACAAAATTCT GAAGATGGCT ACGGATTTGG ACAGCATTTT TATTAACGAA 
ATGGAGTGGG ACAAGCTGCT TCAAGATCTG GTGTGGCGTC ATCAGCCTCT TAAGCTTTCG 
CAGTTACATG TAGAGGGACC GGTCATTTTC GAAGATGTAC TAAGTTTGCA CTCTCTGAAC 
GACCTAAGCT TTCCAGGAGA CTATCTCTGG TCGCAGGGAA ATGAGACGAG TGTTGTCCAG 
GCGCCAAAGG AGTTTACGCA AACGCTTTCT GCAAATGCTG TGGACACGAC GGGAACCATC 
AACGCCGTAT ACCCGCATGA TGTCATTACT CTCAGTGATT CCCAGGAATG GCCGGGATTG 
GTAACCTTCT CGCATTTGGA GGTTTCTGAA GAATTGGAGC TCAATGGCAG CGCCCAGGGA 
AGGCAGTTTG AGGAGGCACC TTTAAATCCC ACTCTGCTGG AGTCACGTGT TATCAGTGCC 
GACTGCCATT TCGAACAGCT TCTTGTGCGT GGCCCTTTGC GGCTGCTTGG AAAGGTGGAT 
AACGACAGCT TTGGCTCTCT GCTAGGAGAT CTCGTTCAGC GTTCGCCGAA CTCTGCCCAA 
GAACTACAGA TAAGTGGACA AAAGCGAGTT GACCAGCTGC TTCTGCCAGT GGATGCTCAT 
ATCGCAGATA ATCAGCTCAG TGGAATTTTC CTCAATGACT TTGTTACCAA GCACACGCCA 
CAAACGCTGT CGAATCTGAG TCAACTGGGA GGGTATGTTT ACTTTCATCA GCTGGAGCTT 
GGGAAAGAGT CCTCTTACGA CGGAGTTCGC CTAGAGGAAC TACTCTCCAA AAGTCTTCGA 
CTAGATAGCC CATTTCCCCT GAGTTCTCCC CACACACGCC TTCGGTTTGT TGGACATGCC 
CCAAATTTCA CCCGGCTACA AGTTGAAAAC ACCTTGAACA AGGTGCCGCT GTCAAGTGGC 
TACCAGCTGC TCCATGAACC GCTCCATCTA GGCAGAGCTA ACTTTAGTCG TCTGATGGCA 
GGGCAAGCGC AGGTTAACTA TGATGTCTCT GGCAACGGGC TACTCAATGG ACGAAGCCTG 
ATCCGTATTC TACAGGAGCA ACCTAGGACT TGGTCGGGTG AAGTGCACGT GCAAGAGCTT 
ATTCTTCCCC AGGGTGTGCA GGCGAACCAG CTGCAGGGCA TTAAGGCAGA CCTTCTGCTG 
GATTTTCTGC AGCAGCTGGA CGAGCTCCCG TTGCTGATCC TTCAGGGTCG CATCCAAGTT 
GAACGCATCG CCGTCAACGG TTCGGTGCAA GCAGCTGAAA CCTTGAACAT GCAACCCTTC 
CACGAACTTC AGAGGCAAGT CGTTTGGCTG GATAGGCAGA ACGAATTAGG GACGCGTTGG 
GTTCTGAAAG AATCTCCCCA GTTCCAGCAG AACCTCCAAG TTCTGGGCAG CTTTAACGAG 
CGCCTGCTCC CAGAATTGCT GGATGACATT GTGCTGCGCT CCGACGCGCA CGAGGTGCTG 
ATCGAGGGCA CCAAAAGCTT CTTGGCTCCT GTTAACACTA AAGAGCTTCA TCTGGCTGCA 
CTGAATGGGA TTCCCTTCAA TCACATGGCC AACAAATTAT CTCCGCTGCA CCTCACTGGA 
AATGTCCGCA TTCAGGGACG CATCTTTGTG GAGGACCTGC AGCTAAATGG TCAAGTAAAC 
GGAGATCCGG ACATTCCTGC TGATCTGCAG AAACTGCTCC GCTGGGATCC AAATGCAGGT 
GGCTTTGTGC ACCGGGGAGT CGTTGAACTT CCAAAAAAGG AGCTAGAACG CCTAGTGGTT 
TTGGGCCACT TGAAAAATCG CAGCCGTGAG CCCGTTCAGG AACTCTTTGA TCAGCTAGTC 
TTCAAGCAGC AGCGTGGCAT ACACTTGCAT GGTCACAAGA CTTTCACAGG CCGTCTTCGC 
ATTGAAGATG GAGCTCACAT CAAGTTCTTC AATGGACGCA ACCTAGAACA GTTCCTAGAG 
AATTTAATCT ACGTGGATTC GCAGGGAGAG ATACGTTTGG AAACACCTGT TCGCTTTGCG 
GCTGATGTTA AAATGGATCA TCTTGAAGCA GATCGCCTTG TACTGTCGGG AGAGCTGCTT 
AACGGATGCA ATGTGAGCCA GTGGCTCCGG GACACTATTC GTGTCGATCG CGATTTCCAA 
CAGCCTGAGA TTGCGTTTGC AAAGGGATCG CTGGATGGTA ATTTCTTGGA GGTTGAGGAG 
CTTAACGAAG TTAATCTCTC ACTGGTTGTC ACCCGCTTCA CGGAGCAACA GCTAAGCGAT 
CCCCTAGAGG CAGATGATTT GCTCCTAGAT GGCCAGCTGG TGGTAAGGGG CACTGTTAAT 
GGTTACAATC TACCAGAGGA GTATGCCAAC ACTTTAATGA TTAACCCATT GCGAGAGCAG 
CAAGTGGATA CGCCTTTATT GTTGTCTAAC ATTTTTGTCA CCGGACCCCT GGAGGTAACT 
GCTCCAGTTC ATGGTCTAAA CATAAGCGAT GTGGCAATTT TGGGCGAAGA TCCAGTGCTG 
CTGCAATCGC CGCTATATTT CGACACTCTG CATGCTCCGT TTTTGAGAGC GCAACAGAAC 
ATCAATGGAT TTGACTTTAG GGCTTGGTAC GGAGGAAGCT TATGGGCAAG AGGTCGGGAG 
CAGCAGGAAA TAAGCGGGAA CTGGCGCGTA AAGAAACTGC AGGTGAAGCA GGGTGATGAG 
CTGCGACCAC GTCGACAAAC TGCAGAAGAG AGCTACCGGG AGTTCTGCGA GGGTTTGGTC 
AGGATTCTCC TGCCTTACAA GGTCCAGAAG CTGCGGCGGA GATTTGATTT AAATCAGGAA 
AGGGATCAGG AGAATATTCG CCGAGTATTT GCTTTGGAGG CACCCGGGGG CATTAGTTAT 
CTTCTGATCA ACGAGCGAGG TTGCTGGACC CGCATCCACC GCTGGAATGG ATCTTTATTC 
GATCGATCCG GAGCCTTCAA GAGCGGACCT GTGGACGAGG CAGTTGCTTT ACGGGTGGGA 
AACACAAGCT CTAACCAGGA CTTTGCCTTT ATGACCAGCT ACGAGTTGCA GGATGATCAG 
AACGAGCCAA GCTTGAATTG CAGTGCAGTG AAGCCCATTC TGCTGAGCTG GAACCTGAAC 
AAAACAAAGA GCACTACTGA GCACATGGAA CTATCAGCGG ACACCCTAAG AAATCTCAAA 
GATCAATATG AGCAGCTTAA AAACCAGCCA CTCCCTAATC CCAGTTACCA GCAAGCTTTT 
AAGTATCTGC AGCGTCCCAG CATCAAGTCT CAGCTCGGAT CACAGTGGCA AGAGAATAAA 
CATGAACTGA GTCCTTCGGA GATGGCCAGA ATGCGGAGAC GTCTGCTGGA CACCTTGGAA 
TTCCGGTTGC AAGCGGAGGT AAACATTACC CAGTTAAGTA TTCCAGAAAG CGACCTATTC 
GACGAGCACC TTGTGGAGGA TTTTCTGGAG TTGATGCGCC AATTGCGAAC CTTGCGCCGG 
CGTCTAGATA CAGAGACTCT TCCACTGCCA AACACGCCGG CAAGAGTTCT GGCCGCACGG 
AGTGCCCAGC TCATCTGGCC CACGTTGCAG GAGTTGCGAG AGGTTTCGAG TGGAAACGAT 
TCTAGCTTCC AGGAGCAAGT TCTGGAGCAA ACGCTGCTGG ATGTGCTGAT GCTGGCTAAT 
GAAGAAAATG GCTCGGGGGA TGATGAAAAG CTGCATGCGG TGATTCAGAG ATTGAGAAAC 
CTTAAAGGAG AGCTTCAAGG GCAAGAGGAA GACGACGAGG GACCTGCTGC CTCCTATTCG 
GACAGAAATG AGCAGCGTTT GCCGCTACCA GAAGTCGACT GGAGACCCAT TCAAACACTT 
CGCCTCGCTG TGGGACCAGC AAACCGTCCC CGACTTCTTT ACGCCCGGCT GACCGTGGTG 
ACACCAACAG ACGGTCCTCC GCCCACCACG CCCTCCACTG CGCCAGCCGC CCACATCCAA 
CTGCATCATG CAAATGGATC GCTCTTTCAA ACGCTAGCAG GGGAGCATGG AGCACGCCAT 
CTGACATCGT TGCGGGTACG AGATGAGACG CTGCTAGCAT TTATGGAGGG ATGCTGCCGT 
ATAAGAGTGC TTATCTACCG CGGTGTGCAG GGATTCGTGG ACTTTGTCCG TTTCCCCGCA 
CCTGCCCAAG AAGCTAAGAG CGGAGATGGG GAGGTCTTGC AGTTACTATC CCTACGCTTA 
CCTCTCAATC GGCCACCCGG AGCCATGTAC TTATTGGCAG TGGTTCAAGC TCGACGCGTC 
ACTTTCTACG AAGTGGTGGT AGCCGGATTA CTAGAGCCGT GGCTCCAGTG CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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fs(1)N cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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