milt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	milt
Entrez Gene ID	45683
Full Name	milton
Synonyms	CG13777,CG31630,CG43227,Dmel\CG43227,Dmel_CG13777,Dmel_CG31630,Milt,anon-WO0153538.19,l(2)k04704
General protein information	Preferred Names milton Names CG43227 gene product from transcript CG43227-RC CG43227-PA CG43227-PB CG43227-PC CG43227-PD CG43227-PE CG43227-PF milt-PA milt-PB milt-PC milt-PD milt-PE milt-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 27D6-27D6

mRNA and Protein(s)

mRNA	Protein	Name
NM_164738.3	NP_723251.1	milton, isoform B
NM_080765.4	NP_542943.2	milton, isoform C
NM_001258998.3	NP_001245927.1	milton, isoform D
NM_001258999.2	NP_001245928.1	milton, isoform E
NM_001259000.3	NP_001245929.1	milton, isoform F
NM_164736.3	NP_723249.1	milton, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TRAK1	XP_003309782.1
Homo sapiens (human)	TRAK1	NP_001036111.1
Canis lupus familiaris (dog)	TRAK1	XP_542729.2
Drosophila melanogaster (fruit fly)	milt	NP_542943.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008801	XP_314926.4
Mus musculus (house mouse)	Trak1	NP_780323.2
Rattus norvegicus (Norway rat)	Trak1	NP_001128037.1
Gallus gallus (chicken)	TRAK1	XP_418813.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A new mode of mitochondrial sorting in polarized cell growth, whereby bi-directional mitochondrial transport undertaken exclusively by dynein is regulated by Milton in the anterograde direction and by a Miro-dependent switch to the retrograde direction.
Title: A new mode of mitochondrial transport and polarized sorting regulated by Dynein, Milton and Miro.

functions in the anchoring, unfurling, and elongation of mitochondria during sperm formation
Title: Roles for Drp1, a dynamin-related protein, and milton, a kinesin-associated protein, in mitochondrial segregation, unfurling and elongation during Drosophila spermatogenesis.

Milton controls the early acquisition of mitochondria by Drosophila oocytes
Title: Milton controls the early acquisition of mitochondria by Drosophila oocytes.

Thus, milton and miro are likely to form an essential protein complex that links KHC to mitochondria for light chain-independent, anterograde transport of mitochondria.
Title: Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent.

The following milt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the milt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa16854	NM_001258999.2 Latest version!	Drosophila melanogaster milton (milt), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa15224	NM_164736.3 Latest version!	Drosophila melanogaster milton (milt), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa15224	NM_080765.4 Latest version!	Drosophila melanogaster milton (milt), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa15225	NM_164738.3 Latest version!	Drosophila melanogaster milton (milt), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa16853	NM_001259000.3 Latest version!	Drosophila melanogaster milton (milt), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa16853	NM_001258998.3 Latest version!	Drosophila melanogaster milton (milt), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

milt ( NM_001258999.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245928.1 Drosophila melanogaster milton (milt), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa16854
Clone ID Related Accession (Same CDS sequence)	NM_001258999.2
Accession Version	NM_001258999.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	milton, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001258999.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTCGGG CGTACGATGA CATTGAGGCC GTGACGCGAC TGCTGGAGGA GAAGGAGAAG 
GACCTAGAGC TGACCGTGCA GATTGGCAAA GAGCTGCTCA CCCAGAACAA TGCACTCGAG 
GCACGGGTCG CGGATCTGGA AACCGATCTC AAGGCCTCCA ACGACGATCG TGCCCAGCTG 
GTCCACGAGC TGCACAAGAA GAACGAGCTC ATCTCTGTGC TTACCAACGA TGCAGACGAT 
GGCACAGATA CTGACACTCC CACCATGTCG AAGTCTATAA CTTTGGATCT ACTGCAGCGC 
AAGGTCAACT CCCTGCTCGA CGAGAACAAG TCTCTGAAAT GCGAAGCCAC CCAACTGGCC 
CACCAGACGG ACGAGGTGGA GGAGCACGAG CGCCAGCTGA TGGCCGATAT CAGTGCTCAG 
CTAAACGATG CTAACTCACA GTACGACAAC CTCAGTTTGG AGTTGGAACG GCAGCGGGAG 
GAAAACCGGC TGCAACATGA GCAGATCGTG AACCTGACCG CTCGCCTAGC GGAGGCGGAA 
ATGCGACTAC ACCAACTGAC GCAAGACAAC GACGAACATC TCTCGCTGCT GCACGTGACG 
AAAGAAAACC AAAATGCTTT GGCCCTGGAA CTGGTGGAGT TCAAGCAGCG TTACGAAGAG 
GTGCTCGCTC TGCTGCACTC TGCTCAGGAT CAGCTGAAGC AACAGCGAAA GCGGTCGCAG 
CCACAGGCAA GGAGCTCCTT CCTCGGTGGC CTTGGAACAA GTGGCGCTGG CATGGGCGGC 
AGTCTTTTCC CGCCGGATTC ACTGCACTGT GAGCTTATGG AGTCTTCACT GTACTCAGAG 
AATAGCCTAG ACTCTGGCAT ATCCGGCGAC AGTCAGCGAT CAGCGGACCG CATTAGCCGC 
ATGATGATGC ACATGCCGTC CGGCGGCATG AGCTCGTCGA CAATGGGAGG TAGCGTGTAT 
GCGGGAGCTG GCAATGTGCC GCCATACAAG CGGGTATTCG ACACAGTGCG ATGTGCCGGC 
AAGAGCGGCA ACTACATGGA CAGCGGCAAC GTGTCGATGA CGCAACTGGG AGCTATGTCG 
ATGAGCAGCT CTTCGGGGCC GCGCATGGCT TCGATGGCGT ATCCAGCGGG CTCCTACTAT 
CGTGGCGGTA GCAATCAATC TCTGGGAGTT AAAACTCTGT CAAGCGAGAG TCTCAACTCC 
CAGTCGGATG ACGGTTATCC AGCCCAGCCC TCTGGTGTGC CAGGAGCGCC CGGCGCCAAG 
GAGCTGGAGG CGGCTCTCAA GAGGCTGACG CCGGCTGAGG TCTTGGCTCG CCGTGCTATG 
TTGTCCTATG CTCCGGCTGG AACCTATAAC TACGACGAAC CAATGGGTCA CGGTACAGGT 
AACGTTCGAA ACTCGGATCT ACCGCTGGGT GTGCGCACGC CGGACAGTAT TATGTCCACC 
GGCTCGTCGG GAATGTCGGG CTCCACGAAT CACATGTCCG CTTCGATGAC GCATCAGTGG 
CGACTACCCG AGAAGCTGCA GATCATCAAA CCCATGGAGG GATCGCAGAC ATTGCACCAT 
TGGTCGCGCT TGGCCACGCC CACGCTCAGT GGACTGCTGG ATGAGCGTCC CGGCGTAACG 
ATCCGTGGTG GACGTGGCCT GGATGATCTG GGAATGCAGA TATACACGCT GTCGGACGTA 
GAGGAGGATG TTAGCGATGA TCTGCCCGGC AAACAGTTTG AAGCACCGGG CTGCACGTTC 
ACCTACACCA ATAGCATGGT CATGCATCCG GACGATGGAT TCGTTAACGA TCTGTCATTC 
CTCTCGCAGT CGCAGATGTC GTCCCGAATG GCCTCCACGT CGACATCAAG GCAACCCAGT 
TGTCCTGCCA CGCCTCGCGC TGGACTATCG CGCAAAAATT CCTGCTCCAC ATTTTCGGTG 
AACCTCGGAC TCGCTGGAAT GCTGAATGAG AGGGGCATCA AGGCGGTGAC GCCCAGTGCC 
CTCAACACGC CCGCCGGACC TAACTTTTCG CCCACGGTGA CGCCATGCAA CAGCCCGGAG 
GGATCACCTC CTCGTGCCCA GTCGCCCGAG CCGCTCTTTG GTCTGCTCTC GTGTGGTGCG 
GATTTGATCC GGCGCAAAAT CGTAGGCGAT CAGCATCAGC AGCAGCAACA GAAGCAAAGG 
AGCAGCCTTA GCAAGCAGCA GCAGCAGAAG ATCATGCTCT CGCACCTGGA AAGACGTGCC 
CTGCGATCGC TGAACCTAAT TGAGAAGGTG GAGAGCATCG GACTGGAGAA CATAATAAGC 
GCACAGAGAG GCCTTGGCTC AGGAATCGCG AACCGCAGCA GTTCGCCCCT GAGCAGTGGC 
AGCCTACAGA GTCTCCACAC CAGCAGCAAC AGCATTGTGG ACGACATACA CTTCGATCGG 
GCACAGATCA AGGGTGTCCT GCATCGAGGC CTGAAATCGC CAACGCCCGC AACAGCATCA 
ACATCTGCAG CTGCGGCGGC TTCAGGGCCA GGTATCTCGA CGAGCAGCAG CACGAGTGCT 
TACAATAGCG ATGATAGCGA CGATCAGGGC TTGGTAATGA AAATCAAACC GTCGAAGAGC 
GCGACACCCA CAACAACAGG AGCAGCACAA AGTCAGCCAA GTTCGGCGAC GACATCCAAC 
GGCACGGCGA GTGAAACGCG ACTGAAGCAG ATGCAGCGCC AGAAATCGCG CAGGCAGCTA 
AAGAACGGAA TGGCCAACCA GAGGCCAGAT CTGGGCACAA TTTCTGGCGC CGGAGGAGGC 
GGACGAGTGC GCCCGGATCT GGGAAAGGTA GCCGACAGCG GCAGCAGCAG CAAGCTCAGC 
ACCAAGCGAA GTGAGGCTAA GCCTGCGGAG GAGGAAGAGG CGACACCACA GACCATCACA 
CAGGCGTTTG TGGGTTCAGT TAGTTCCTTG CTTTTTGGCC GCAAGGGCGG TTGGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245928.1
CDS	481..3420
Translation	MTRAYDDIEAVTRLLEEKEKDLELTVQIGKELLTQNNALEARVADLETDLKASNDDRAQLVHELHKKNELISVLTNDADDGTDTDTPTMSKSITLDLLQRKVNSLLDENKSLKCEATQLAHQTDEVEEHERQLMADISAQLNDANSQYDNLSLELERQREENRLQHEQIVNLTARLAEAEMRLHQLTQDNDEHLSLLHVTKENQNALALELVEFKQRYEEVLALLHSAQDQLKQQRKRSQPQARSSFLGGLGTSGAGMGGSLFPPDSLHCELMESSLYSENSLDSGISGDSQRSADRISRMMMHMPSGGMSSSTMGGSVYAGAGNVPPYKRVFDTVRCAGKSGNYMDSGNVSMTQLGAMSMSSSSGPRMASMAYPAGSYYRGGSNQSLGVKTLSSESLNSQSDDGYPAQPSGVPGAPGAKELEAALKRLTPAEVLARRAMLSYAPAGTYNYDEPMGHGTGNVRNSDLPLGVRTPDSIMSTGSSGMSGSTNHMSASMTHQWRLPEKLQIIKPMEGSQTLHHWSRLATPTLSGLLDERPGVTIRGGRGLDDLGMQIYTLSDVEEDVSDDLPGKQFEAPGCTFTYTNSMVMHPDDGFVNDLSFLSQSQMSSRMASTSTSRQPSCPATPRAGLSRKNSCSTFSVNLGLAGMLNERGIKAVTPSALNTPAGPNFSPTVTPCNSPEGSPPRAQSPEPLFGLLSCGADLIRRKIVGDQHQQQQQKQRSSLSKQQQQKIMLSHLERRALRSLNLIEKVESIGLENIISAQRGLGSGIANRSSSPLSSGSLQSLHTSSNSIVDDIHFDRAQIKGVLHRGLKSPTPATASTSAAAAASGPGISTSSSTSAYNSDDSDDQGLVMKIKPSKSATPTTTGAAQSQPSSATTSNGTASETRLKQMQRQKSRRQLKNGMANQRPDLGTISGAGGGGRVRPDLGKVADSGSSSKLSTKRSEAKPAEEEEATPQTITQAFVGSVSSLLFGRKGGWL

Target ORF information:

RefSeq Version	NM_001258999.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster milton (milt), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258999.2

ORF Insert Sequence:

ATGACTCGGG CGTACGATGA CATTGAGGCC GTGACGCGAC TGCTGGAGGA GAAGGAGAAG 
GACCTAGAGC TGACCGTGCA GATTGGCAAA GAGCTGCTCA CCCAGAACAA TGCACTCGAG 
GCACGGGTCG CGGATCTGGA AACCGATCTC AAGGCCTCCA ACGACGATCG TGCCCAGCTG 
GTCCACGAGC TGCACAAGAA GAACGAGCTC ATCTCTGTGC TTACCAACGA TGCAGACGAT 
GGCACAGATA CTGACACTCC CACCATGTCG AAGTCTATAA CTTTGGATCT ACTGCAGCGC 
AAGGTCAACT CCCTGCTCGA CGAGAACAAG TCTCTGAAAT GCGAAGCCAC CCAACTGGCC 
CACCAGACGG ACGAGGTGGA GGAGCACGAG CGCCAGCTGA TGGCCGATAT CAGTGCTCAG 
CTAAACGATG CTAACTCACA GTACGACAAC CTCAGTTTGG AGTTGGAACG GCAGCGGGAG 
GAAAACCGGC TGCAACATGA GCAGATCGTG AACCTGACCG CTCGCCTAGC GGAGGCGGAA 
ATGCGACTAC ACCAACTGAC GCAAGACAAC GACGAACATC TCTCGCTGCT GCACGTGACG 
AAAGAAAACC AAAATGCTTT GGCCCTGGAA CTGGTGGAGT TCAAGCAGCG TTACGAAGAG 
GTGCTCGCTC TGCTGCACTC TGCTCAGGAT CAGCTGAAGC AACAGCGAAA GCGGTCGCAG 
CCACAGGCAA GGAGCTCCTT CCTCGGTGGC CTTGGAACAA GTGGCGCTGG CATGGGCGGC 
AGTCTTTTCC CGCCGGATTC ACTGCACTGT GAGCTTATGG AGTCTTCACT GTACTCAGAG 
AATAGCCTAG ACTCTGGCAT ATCCGGCGAC AGTCAGCGAT CAGCGGACCG CATTAGCCGC 
ATGATGATGC ACATGCCGTC CGGCGGCATG AGCTCGTCGA CAATGGGAGG TAGCGTGTAT 
GCGGGAGCTG GCAATGTGCC GCCATACAAG CGGGTATTCG ACACAGTGCG ATGTGCCGGC 
AAGAGCGGCA ACTACATGGA CAGCGGCAAC GTGTCGATGA CGCAACTGGG AGCTATGTCG 
ATGAGCAGCT CTTCGGGGCC GCGCATGGCT TCGATGGCGT ATCCAGCGGG CTCCTACTAT 
CGTGGCGGTA GCAATCAATC TCTGGGAGTT AAAACTCTGT CAAGCGAGAG TCTCAACTCC 
CAGTCGGATG ACGGTTATCC AGCCCAGCCC TCTGGTGTGC CAGGAGCGCC CGGCGCCAAG 
GAGCTGGAGG CGGCTCTCAA GAGGCTGACG CCGGCTGAGG TCTTGGCTCG CCGTGCTATG 
TTGTCCTATG CTCCGGCTGG AACCTATAAC TACGACGAAC CAATGGGTCA CGGTACAGGT 
AACGTTCGAA ACTCGGATCT ACCGCTGGGT GTGCGCACGC CGGACAGTAT TATGTCCACC 
GGCTCGTCGG GAATGTCGGG CTCCACGAAT CACATGTCCG CTTCGATGAC GCATCAGTGG 
CGACTACCCG AGAAGCTGCA GATCATCAAA CCCATGGAGG GATCGCAGAC ATTGCACCAT 
TGGTCGCGCT TGGCCACGCC CACGCTCAGT GGACTGCTGG ATGAGCGTCC CGGCGTAACG 
ATCCGTGGTG GACGTGGCCT GGATGATCTG GGAATGCAGA TATACACGCT GTCGGACGTA 
GAGGAGGATG TTAGCGATGA TCTGCCCGGC AAACAGTTTG AAGCACCGGG CTGCACGTTC 
ACCTACACCA ATAGCATGGT CATGCATCCG GACGATGGAT TCGTTAACGA TCTGTCATTC 
CTCTCGCAGT CGCAGATGTC GTCCCGAATG GCCTCCACGT CGACATCAAG GCAACCCAGT 
TGTCCTGCCA CGCCTCGCGC TGGACTATCG CGCAAAAATT CCTGCTCCAC ATTTTCGGTG 
AACCTCGGAC TCGCTGGAAT GCTGAATGAG AGGGGCATCA AGGCGGTGAC GCCCAGTGCC 
CTCAACACGC CCGCCGGACC TAACTTTTCG CCCACGGTGA CGCCATGCAA CAGCCCGGAG 
GGATCACCTC CTCGTGCCCA GTCGCCCGAG CCGCTCTTTG GTCTGCTCTC GTGTGGTGCG 
GATTTGATCC GGCGCAAAAT CGTAGGCGAT CAGCATCAGC AGCAGCAACA GAAGCAAAGG 
AGCAGCCTTA GCAAGCAGCA GCAGCAGAAG ATCATGCTCT CGCACCTGGA AAGACGTGCC 
CTGCGATCGC TGAACCTAAT TGAGAAGGTG GAGAGCATCG GACTGGAGAA CATAATAAGC 
GCACAGAGAG GCCTTGGCTC AGGAATCGCG AACCGCAGCA GTTCGCCCCT GAGCAGTGGC 
AGCCTACAGA GTCTCCACAC CAGCAGCAAC AGCATTGTGG ACGACATACA CTTCGATCGG 
GCACAGATCA AGGGTGTCCT GCATCGAGGC CTGAAATCGC CAACGCCCGC AACAGCATCA 
ACATCTGCAG CTGCGGCGGC TTCAGGGCCA GGTATCTCGA CGAGCAGCAG CACGAGTGCT 
TACAATAGCG ATGATAGCGA CGATCAGGGC TTGGTAATGA AAATCAAACC GTCGAAGAGC 
GCGACACCCA CAACAACAGG AGCAGCACAA AGTCAGCCAA GTTCGGCGAC GACATCCAAC 
GGCACGGCGA GTGAAACGCG ACTGAAGCAG ATGCAGCGCC AGAAATCGCG CAGGCAGCTA 
AAGAACGGAA TGGCCAACCA GAGGCCAGAT CTGGGCACAA TTTCTGGCGC CGGAGGAGGC 
GGACGAGTGC GCCCGGATCT GGGAAAGGTA GCCGACAGCG GCAGCAGCAG CAAGCTCAGC 
ACCAAGCGAA GTGAGGCTAA GCCTGCGGAG GAGGAAGAGG CGACACCACA GACCATCACA 
CAGGCGTTTG TGGGTTCAGT TAGTTCCTTG CTTTTTGGCC GCAAGGGCGG TTGGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

milt ( NM_080765.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_542943.2 Drosophila melanogaster milton (milt), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa15224
Clone ID Related Accession (Same CDS sequence)	NM_080765.4 , NM_164736.3
Accession Version	NM_080765.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	milton, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_080765.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTATCCG CAACTTTGGG GGCCAATGGC GGCTCTCAGC CGCTCAGTCC CTCCGCCCAG 
GCGACGCTCA GCAAGCACCT GCCGCGCCTC CAGTCGAAGC GTCTTCTCCA GCAGACGCAG 
TTGCAGTCGC CGGCTGCCGC CAGACCACAG ACCTGCGATG CCAGCTGCCT GACGGAGCTG 
TGCAGCAGCG AGAATTTGCC CGAGGTGGAG ATTTTCTCAC TGCTGGAGGA GCAGATACCC 
AAGTACAAGG TGCGCAACGA TTTCCTCACA AACTTCTCGG GCTATGCGAA TGAGGACTGG 
TTCGTTCCGG CACCAGCGCT GCCCATTCCG CCGGAGGGTC TGGGCTTGAC CAAGGAGCAG 
ACAAGAGAGT GCCTAAACTA TTTCCTTCTA TGCGGCAACC GCGTTAGCCA AATGACTCGG 
GCGTACGATG ACATTGAGGC CGTGACGCGA CTGCTGGAGG AGAAGGAGAA GGACCTAGAG 
CTGACCGTGC AGATTGGCAA AGAGCTGCTC ACCCAGAACA ATGCACTCGA GGCACGGGTC 
GCGGATCTGG AAACCGATCT CAAGGCCTCC AACGACGATC GTGCCCAGCT GGTCCACGAG 
CTGCACAAGA AGAACGAGCT CATCTCTGTG CTTACCAACG ATGCAGACGA TGGCACAGAT 
ACTGACACTC CCACCATGTC GAAGTCTATA ACTTTGGATC TACTGCAGCG CAAGGTCAAC 
TCCCTGCTCG ACGAGAACAA GTCTCTGAAA TGCGAAGCCA CCCAACTGGC CCACCAGACG 
GACGAGGTGG AGGAGCACGA GCGCCAGCTG ATGGCCGATA TCAGTGCTCA GCTAAACGAT 
GCTAACTCAC AGTACGACAA CCTCAGTTTG GAGTTGGAAC GGCAGCGGGA GGAAAACCGG 
CTGCAACATG AGCAGATCGT GAACCTGACC GCTCGCCTAG CGGAGGCGGA AATGCGACTA 
CACCAACTGA CGCAAGACAA CGACGAACAT CTCTCGCTGC TGCACGTGAC GAAAGAAAAC 
CAAAATGCTT TGGCCCTGGA ACTGGTGGAG TTCAAGCAGC GTTACGAAGA GGTGCTCGCT 
CTGCTGCACT CTGCTCAGGA TCAGCTGAAG CAACAGCGAA AGCGGTCGCA GCCACAGGCA 
AGGAGCTCCT TCCTCGGTGG CCTTGGAACA AGTGGCGCTG GCATGGGCGG CAGTCTTTTC 
CCGCCGGATT CACTGCACTG TGAGCTTATG GAGTCTTCAC TGTACTCAGA GAATAGCCTA 
GACTCTGGCA TATCCGGCGA CAGTCAGCGA TCAGCGGACC GCATTAGCCG CATGATGATG 
CACATGCCGT CCGGCGGCAT GAGCTCGTCG ACAATGGGAG GTAGCGTGTA TGCGGGAGCT 
GGCAATGTGC CGCCATACAA GCGGGTATTC GACACAGTGC GATGTGCCGG CAAGAGCGGC 
AACTACATGG ACAGCGGCAA CGTGTCGATG ACGCAACTGG GAGCTATGTC GATGAGCAGC 
TCTTCGGGGC CGCGCATGGC TTCGATGGCG TATCCAGCGG GCTCCTACTA TCGTGGCGGT 
AGCAATCAAT CTCTGGGAGT TAAAACTCTG TCAAGCGAGA GTCTCAACTC CCAGTCGGAT 
GACGGTTATC CAGCCCAGCC CTCTGGTGTG CCAGGAGCGC CCGGCGCCAA GGAGCTGGAG 
GCGGCTCTCA AGAGGCTGAC GCCGGCTGAG GTCTTGGCTC GCCGTGCTAT GTTGTCCTAT 
GCTCCGGCTG GAACCTATAA CTACGACGAA CCAATGGGTC ACGGTACAGG TAACGTTCGA 
AACTCGGATC TACCGCTGGG TGTGCGCACG CCGGACAGTA TTATGTCCAC CGGCTCGTCG 
GGAATGTCGG GCTCCACGAA TCACATGTCC GCTTCGATGA CGCATCAGTG GCGACTACCC 
GAGAAGCTGC AGATCATCAA ACCCATGGAG GGATCGCAGA CATTGCACCA TTGGTCGCGC 
TTGGCCACGC CCACGCTCAG TGGACTGCTG GATGAGCGTC CCGGCGTAAC GATCCGTGGT 
GGACGTGGCC TGGATGATCT GGGAATGCAG ATATACACGC TGTCGGACGT AGAGGAGGAT 
GTTAGCGATG ATCTGCCCGG CAAACAGTTT GAAGCACCGG GCTGCACGTT CACCTACACC 
AATAGCATGG TCATGCATCC GGACGATGGA TTCGTTAACG ATCTGTCATT CCTCTCGCAG 
TCGCAGATGT CGTCCCGAAT GGCCTCCACG TCGACATCAA GGCAACCCAG TTGTCCTGCC 
ACGCCTCGCG CTGGACTATC GCGCAAAAAT TCCTGCTCCA CATTTTCGGT GAACCTCGGA 
CTCGCTGGAA TGCTGAATGA GAGGGGCATC AAGGCGGTGA CGCCCAGTGC CCTCAACACG 
CCCGCCGGAC CTAACTTTTC GCCCACGGTG ACGCCATGCA ACAGCCCGGA GGGATCACCT 
CCTCGTGCCC AGTCGCCCGA GCCGCTCTTT GGTCTGCTCT CGTGTGGTGC GGATTTGATC 
CGGCGCAAAA TCGTAGGCGA TCAGCATCAG CAGCAGCAAC AGAAGCAAAG GAGCAGCCTT 
AGCAAGCAGC AGCAGCAGAA GATCATGCTC TCGCACCTGG AAAGACGTGC CCTGCGATCG 
CTGAACCTAA TTGAGAAGGT GGAGAGCATC GGACTGGAGA ACATAATAAG CGCACAGAGA 
GGCCTTGGCT CAGGAATCGC GAACCGCAGC AGTTCGCCCC TGAGCAGTGG CAGCCTACAG 
AGTCTCCACA CCAGCAGCAA CAGCATTGTG GACGACATAC ACTTCGATCG GGCACAGATC 
AAGGGTGTCC TGCATCGAGG CCTGAAATCG CCAACGCCCG CAACAGCATC AACATCTGCA 
GCTGCGGCGG CTTCAGGGCC AGGTATCTCG ACGAGCAGCA GCACGAGTGC TTACAATAGC 
GATGATAGCG ACGATCAGGG CTTGGTAATG AAAATCAAAC CGTCGAAGAG CGCGACACCC 
ACAACAACAG GAGCAGCACA AAGTCAGCCA AGTTCGGCGA CGACATCCAA CGGCACGGCG 
AGTGAAACGC GACTGAAGCA GATGCAGCGC CAGAAATCGC GCAGGCAGCT AAAGAACGGA 
ATGGCCAACC AGAGGCCAGA TCTGGGCACA ATTTCTGGCG CCGGAGGAGG CGGACGAGTG 
CGCCCGGATC TGGGAAAGGT AGCCGACAGC GGCAGCAGCA GCAAGCTCAG CACCAAGCGA 
AGTGAGGCTA AGCCTGCGGA GGAGGAAGAG GCGACACCAC AGACCATCAC ACAGGCGTTT 
GTGGGTTCAG TTAGTTCCTT GCTTTTTGGC CGCAAGGGCG GTTGGCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542943.2
CDS	678..4028
Translation	MLSATLGANGGSQPLSPSAQATLSKHLPRLQSKRLLQQTQLQSPAAARPQTCDASCLTELCSSENLPEVEIFSLLEEQIPKYKVRNDFLTNFSGYANEDWFVPAPALPIPPEGLGLTKEQTRECLNYFLLCGNRVSQMTRAYDDIEAVTRLLEEKEKDLELTVQIGKELLTQNNALEARVADLETDLKASNDDRAQLVHELHKKNELISVLTNDADDGTDTDTPTMSKSITLDLLQRKVNSLLDENKSLKCEATQLAHQTDEVEEHERQLMADISAQLNDANSQYDNLSLELERQREENRLQHEQIVNLTARLAEAEMRLHQLTQDNDEHLSLLHVTKENQNALALELVEFKQRYEEVLALLHSAQDQLKQQRKRSQPQARSSFLGGLGTSGAGMGGSLFPPDSLHCELMESSLYSENSLDSGISGDSQRSADRISRMMMHMPSGGMSSSTMGGSVYAGAGNVPPYKRVFDTVRCAGKSGNYMDSGNVSMTQLGAMSMSSSSGPRMASMAYPAGSYYRGGSNQSLGVKTLSSESLNSQSDDGYPAQPSGVPGAPGAKELEAALKRLTPAEVLARRAMLSYAPAGTYNYDEPMGHGTGNVRNSDLPLGVRTPDSIMSTGSSGMSGSTNHMSASMTHQWRLPEKLQIIKPMEGSQTLHHWSRLATPTLSGLLDERPGVTIRGGRGLDDLGMQIYTLSDVEEDVSDDLPGKQFEAPGCTFTYTNSMVMHPDDGFVNDLSFLSQSQMSSRMASTSTSRQPSCPATPRAGLSRKNSCSTFSVNLGLAGMLNERGIKAVTPSALNTPAGPNFSPTVTPCNSPEGSPPRAQSPEPLFGLLSCGADLIRRKIVGDQHQQQQQKQRSSLSKQQQQKIMLSHLERRALRSLNLIEKVESIGLENIISAQRGLGSGIANRSSSPLSSGSLQSLHTSSNSIVDDIHFDRAQIKGVLHRGLKSPTPATASTSAAAAASGPGISTSSSTSAYNSDDSDDQGLVMKIKPSKSATPTTTGAAQSQPSSATTSNGTASETRLKQMQRQKSRRQLKNGMANQRPDLGTISGAGGGGRVRPDLGKVADSGSSSKLSTKRSEAKPAEEEEATPQTITQAFVGSVSSLLFGRKGGWL

Target ORF information:

RefSeq Version	NM_080765.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster milton (milt), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080765.4

ORF Insert Sequence:

ATGCTATCCG CAACTTTGGG GGCCAATGGC GGCTCTCAGC CGCTCAGTCC CTCCGCCCAG 
GCGACGCTCA GCAAGCACCT GCCGCGCCTC CAGTCGAAGC GTCTTCTCCA GCAGACGCAG 
TTGCAGTCGC CGGCTGCCGC CAGACCACAG ACCTGCGATG CCAGCTGCCT GACGGAGCTG 
TGCAGCAGCG AGAATTTGCC CGAGGTGGAG ATTTTCTCAC TGCTGGAGGA GCAGATACCC 
AAGTACAAGG TGCGCAACGA TTTCCTCACA AACTTCTCGG GCTATGCGAA TGAGGACTGG 
TTCGTTCCGG CACCAGCGCT GCCCATTCCG CCGGAGGGTC TGGGCTTGAC CAAGGAGCAG 
ACAAGAGAGT GCCTAAACTA TTTCCTTCTA TGCGGCAACC GCGTTAGCCA AATGACTCGG 
GCGTACGATG ACATTGAGGC CGTGACGCGA CTGCTGGAGG AGAAGGAGAA GGACCTAGAG 
CTGACCGTGC AGATTGGCAA AGAGCTGCTC ACCCAGAACA ATGCACTCGA GGCACGGGTC 
GCGGATCTGG AAACCGATCT CAAGGCCTCC AACGACGATC GTGCCCAGCT GGTCCACGAG 
CTGCACAAGA AGAACGAGCT CATCTCTGTG CTTACCAACG ATGCAGACGA TGGCACAGAT 
ACTGACACTC CCACCATGTC GAAGTCTATA ACTTTGGATC TACTGCAGCG CAAGGTCAAC 
TCCCTGCTCG ACGAGAACAA GTCTCTGAAA TGCGAAGCCA CCCAACTGGC CCACCAGACG 
GACGAGGTGG AGGAGCACGA GCGCCAGCTG ATGGCCGATA TCAGTGCTCA GCTAAACGAT 
GCTAACTCAC AGTACGACAA CCTCAGTTTG GAGTTGGAAC GGCAGCGGGA GGAAAACCGG 
CTGCAACATG AGCAGATCGT GAACCTGACC GCTCGCCTAG CGGAGGCGGA AATGCGACTA 
CACCAACTGA CGCAAGACAA CGACGAACAT CTCTCGCTGC TGCACGTGAC GAAAGAAAAC 
CAAAATGCTT TGGCCCTGGA ACTGGTGGAG TTCAAGCAGC GTTACGAAGA GGTGCTCGCT 
CTGCTGCACT CTGCTCAGGA TCAGCTGAAG CAACAGCGAA AGCGGTCGCA GCCACAGGCA 
AGGAGCTCCT TCCTCGGTGG CCTTGGAACA AGTGGCGCTG GCATGGGCGG CAGTCTTTTC 
CCGCCGGATT CACTGCACTG TGAGCTTATG GAGTCTTCAC TGTACTCAGA GAATAGCCTA 
GACTCTGGCA TATCCGGCGA CAGTCAGCGA TCAGCGGACC GCATTAGCCG CATGATGATG 
CACATGCCGT CCGGCGGCAT GAGCTCGTCG ACAATGGGAG GTAGCGTGTA TGCGGGAGCT 
GGCAATGTGC CGCCATACAA GCGGGTATTC GACACAGTGC GATGTGCCGG CAAGAGCGGC 
AACTACATGG ACAGCGGCAA CGTGTCGATG ACGCAACTGG GAGCTATGTC GATGAGCAGC 
TCTTCGGGGC CGCGCATGGC TTCGATGGCG TATCCAGCGG GCTCCTACTA TCGTGGCGGT 
AGCAATCAAT CTCTGGGAGT TAAAACTCTG TCAAGCGAGA GTCTCAACTC CCAGTCGGAT 
GACGGTTATC CAGCCCAGCC CTCTGGTGTG CCAGGAGCGC CCGGCGCCAA GGAGCTGGAG 
GCGGCTCTCA AGAGGCTGAC GCCGGCTGAG GTCTTGGCTC GCCGTGCTAT GTTGTCCTAT 
GCTCCGGCTG GAACCTATAA CTACGACGAA CCAATGGGTC ACGGTACAGG TAACGTTCGA 
AACTCGGATC TACCGCTGGG TGTGCGCACG CCGGACAGTA TTATGTCCAC CGGCTCGTCG 
GGAATGTCGG GCTCCACGAA TCACATGTCC GCTTCGATGA CGCATCAGTG GCGACTACCC 
GAGAAGCTGC AGATCATCAA ACCCATGGAG GGATCGCAGA CATTGCACCA TTGGTCGCGC 
TTGGCCACGC CCACGCTCAG TGGACTGCTG GATGAGCGTC CCGGCGTAAC GATCCGTGGT 
GGACGTGGCC TGGATGATCT GGGAATGCAG ATATACACGC TGTCGGACGT AGAGGAGGAT 
GTTAGCGATG ATCTGCCCGG CAAACAGTTT GAAGCACCGG GCTGCACGTT CACCTACACC 
AATAGCATGG TCATGCATCC GGACGATGGA TTCGTTAACG ATCTGTCATT CCTCTCGCAG 
TCGCAGATGT CGTCCCGAAT GGCCTCCACG TCGACATCAA GGCAACCCAG TTGTCCTGCC 
ACGCCTCGCG CTGGACTATC GCGCAAAAAT TCCTGCTCCA CATTTTCGGT GAACCTCGGA 
CTCGCTGGAA TGCTGAATGA GAGGGGCATC AAGGCGGTGA CGCCCAGTGC CCTCAACACG 
CCCGCCGGAC CTAACTTTTC GCCCACGGTG ACGCCATGCA ACAGCCCGGA GGGATCACCT 
CCTCGTGCCC AGTCGCCCGA GCCGCTCTTT GGTCTGCTCT CGTGTGGTGC GGATTTGATC 
CGGCGCAAAA TCGTAGGCGA TCAGCATCAG CAGCAGCAAC AGAAGCAAAG GAGCAGCCTT 
AGCAAGCAGC AGCAGCAGAA GATCATGCTC TCGCACCTGG AAAGACGTGC CCTGCGATCG 
CTGAACCTAA TTGAGAAGGT GGAGAGCATC GGACTGGAGA ACATAATAAG CGCACAGAGA 
GGCCTTGGCT CAGGAATCGC GAACCGCAGC AGTTCGCCCC TGAGCAGTGG CAGCCTACAG 
AGTCTCCACA CCAGCAGCAA CAGCATTGTG GACGACATAC ACTTCGATCG GGCACAGATC 
AAGGGTGTCC TGCATCGAGG CCTGAAATCG CCAACGCCCG CAACAGCATC AACATCTGCA 
GCTGCGGCGG CTTCAGGGCC AGGTATCTCG ACGAGCAGCA GCACGAGTGC TTACAATAGC 
GATGATAGCG ACGATCAGGG CTTGGTAATG AAAATCAAAC CGTCGAAGAG CGCGACACCC 
ACAACAACAG GAGCAGCACA AAGTCAGCCA AGTTCGGCGA CGACATCCAA CGGCACGGCG 
AGTGAAACGC GACTGAAGCA GATGCAGCGC CAGAAATCGC GCAGGCAGCT AAAGAACGGA 
ATGGCCAACC AGAGGCCAGA TCTGGGCACA ATTTCTGGCG CCGGAGGAGG CGGACGAGTG 
CGCCCGGATC TGGGAAAGGT AGCCGACAGC GGCAGCAGCA GCAAGCTCAG CACCAAGCGA 
AGTGAGGCTA AGCCTGCGGA GGAGGAAGAG GCGACACCAC AGACCATCAC ACAGGCGTTT 
GTGGGTTCAG TTAGTTCCTT GCTTTTTGGC CGCAAGGGCG GTTGGCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

milt ( NM_164736.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723249.1 Drosophila melanogaster milton (milt), transcript variant A, mRNA.

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CloneID	OFa15224
Clone ID Related Accession (Same CDS sequence)	NM_080765.4 , NM_164736.3
Accession Version	NM_164736.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	milton, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164736.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTATCCG CAACTTTGGG GGCCAATGGC GGCTCTCAGC CGCTCAGTCC CTCCGCCCAG 
GCGACGCTCA GCAAGCACCT GCCGCGCCTC CAGTCGAAGC GTCTTCTCCA GCAGACGCAG 
TTGCAGTCGC CGGCTGCCGC CAGACCACAG ACCTGCGATG CCAGCTGCCT GACGGAGCTG 
TGCAGCAGCG AGAATTTGCC CGAGGTGGAG ATTTTCTCAC TGCTGGAGGA GCAGATACCC 
AAGTACAAGG TGCGCAACGA TTTCCTCACA AACTTCTCGG GCTATGCGAA TGAGGACTGG 
TTCGTTCCGG CACCAGCGCT GCCCATTCCG CCGGAGGGTC TGGGCTTGAC CAAGGAGCAG 
ACAAGAGAGT GCCTAAACTA TTTCCTTCTA TGCGGCAACC GCGTTAGCCA AATGACTCGG 
GCGTACGATG ACATTGAGGC CGTGACGCGA CTGCTGGAGG AGAAGGAGAA GGACCTAGAG 
CTGACCGTGC AGATTGGCAA AGAGCTGCTC ACCCAGAACA ATGCACTCGA GGCACGGGTC 
GCGGATCTGG AAACCGATCT CAAGGCCTCC AACGACGATC GTGCCCAGCT GGTCCACGAG 
CTGCACAAGA AGAACGAGCT CATCTCTGTG CTTACCAACG ATGCAGACGA TGGCACAGAT 
ACTGACACTC CCACCATGTC GAAGTCTATA ACTTTGGATC TACTGCAGCG CAAGGTCAAC 
TCCCTGCTCG ACGAGAACAA GTCTCTGAAA TGCGAAGCCA CCCAACTGGC CCACCAGACG 
GACGAGGTGG AGGAGCACGA GCGCCAGCTG ATGGCCGATA TCAGTGCTCA GCTAAACGAT 
GCTAACTCAC AGTACGACAA CCTCAGTTTG GAGTTGGAAC GGCAGCGGGA GGAAAACCGG 
CTGCAACATG AGCAGATCGT GAACCTGACC GCTCGCCTAG CGGAGGCGGA AATGCGACTA 
CACCAACTGA CGCAAGACAA CGACGAACAT CTCTCGCTGC TGCACGTGAC GAAAGAAAAC 
CAAAATGCTT TGGCCCTGGA ACTGGTGGAG TTCAAGCAGC GTTACGAAGA GGTGCTCGCT 
CTGCTGCACT CTGCTCAGGA TCAGCTGAAG CAACAGCGAA AGCGGTCGCA GCCACAGGCA 
AGGAGCTCCT TCCTCGGTGG CCTTGGAACA AGTGGCGCTG GCATGGGCGG CAGTCTTTTC 
CCGCCGGATT CACTGCACTG TGAGCTTATG GAGTCTTCAC TGTACTCAGA GAATAGCCTA 
GACTCTGGCA TATCCGGCGA CAGTCAGCGA TCAGCGGACC GCATTAGCCG CATGATGATG 
CACATGCCGT CCGGCGGCAT GAGCTCGTCG ACAATGGGAG GTAGCGTGTA TGCGGGAGCT 
GGCAATGTGC CGCCATACAA GCGGGTATTC GACACAGTGC GATGTGCCGG CAAGAGCGGC 
AACTACATGG ACAGCGGCAA CGTGTCGATG ACGCAACTGG GAGCTATGTC GATGAGCAGC 
TCTTCGGGGC CGCGCATGGC TTCGATGGCG TATCCAGCGG GCTCCTACTA TCGTGGCGGT 
AGCAATCAAT CTCTGGGAGT TAAAACTCTG TCAAGCGAGA GTCTCAACTC CCAGTCGGAT 
GACGGTTATC CAGCCCAGCC CTCTGGTGTG CCAGGAGCGC CCGGCGCCAA GGAGCTGGAG 
GCGGCTCTCA AGAGGCTGAC GCCGGCTGAG GTCTTGGCTC GCCGTGCTAT GTTGTCCTAT 
GCTCCGGCTG GAACCTATAA CTACGACGAA CCAATGGGTC ACGGTACAGG TAACGTTCGA 
AACTCGGATC TACCGCTGGG TGTGCGCACG CCGGACAGTA TTATGTCCAC CGGCTCGTCG 
GGAATGTCGG GCTCCACGAA TCACATGTCC GCTTCGATGA CGCATCAGTG GCGACTACCC 
GAGAAGCTGC AGATCATCAA ACCCATGGAG GGATCGCAGA CATTGCACCA TTGGTCGCGC 
TTGGCCACGC CCACGCTCAG TGGACTGCTG GATGAGCGTC CCGGCGTAAC GATCCGTGGT 
GGACGTGGCC TGGATGATCT GGGAATGCAG ATATACACGC TGTCGGACGT AGAGGAGGAT 
GTTAGCGATG ATCTGCCCGG CAAACAGTTT GAAGCACCGG GCTGCACGTT CACCTACACC 
AATAGCATGG TCATGCATCC GGACGATGGA TTCGTTAACG ATCTGTCATT CCTCTCGCAG 
TCGCAGATGT CGTCCCGAAT GGCCTCCACG TCGACATCAA GGCAACCCAG TTGTCCTGCC 
ACGCCTCGCG CTGGACTATC GCGCAAAAAT TCCTGCTCCA CATTTTCGGT GAACCTCGGA 
CTCGCTGGAA TGCTGAATGA GAGGGGCATC AAGGCGGTGA CGCCCAGTGC CCTCAACACG 
CCCGCCGGAC CTAACTTTTC GCCCACGGTG ACGCCATGCA ACAGCCCGGA GGGATCACCT 
CCTCGTGCCC AGTCGCCCGA GCCGCTCTTT GGTCTGCTCT CGTGTGGTGC GGATTTGATC 
CGGCGCAAAA TCGTAGGCGA TCAGCATCAG CAGCAGCAAC AGAAGCAAAG GAGCAGCCTT 
AGCAAGCAGC AGCAGCAGAA GATCATGCTC TCGCACCTGG AAAGACGTGC CCTGCGATCG 
CTGAACCTAA TTGAGAAGGT GGAGAGCATC GGACTGGAGA ACATAATAAG CGCACAGAGA 
GGCCTTGGCT CAGGAATCGC GAACCGCAGC AGTTCGCCCC TGAGCAGTGG CAGCCTACAG 
AGTCTCCACA CCAGCAGCAA CAGCATTGTG GACGACATAC ACTTCGATCG GGCACAGATC 
AAGGGTGTCC TGCATCGAGG CCTGAAATCG CCAACGCCCG CAACAGCATC AACATCTGCA 
GCTGCGGCGG CTTCAGGGCC AGGTATCTCG ACGAGCAGCA GCACGAGTGC TTACAATAGC 
GATGATAGCG ACGATCAGGG CTTGGTAATG AAAATCAAAC CGTCGAAGAG CGCGACACCC 
ACAACAACAG GAGCAGCACA AAGTCAGCCA AGTTCGGCGA CGACATCCAA CGGCACGGCG 
AGTGAAACGC GACTGAAGCA GATGCAGCGC CAGAAATCGC GCAGGCAGCT AAAGAACGGA 
ATGGCCAACC AGAGGCCAGA TCTGGGCACA ATTTCTGGCG CCGGAGGAGG CGGACGAGTG 
CGCCCGGATC TGGGAAAGGT AGCCGACAGC GGCAGCAGCA GCAAGCTCAG CACCAAGCGA 
AGTGAGGCTA AGCCTGCGGA GGAGGAAGAG GCGACACCAC AGACCATCAC ACAGGCGTTT 
GTGGGTTCAG TTAGTTCCTT GCTTTTTGGC CGCAAGGGCG GTTGGCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723249.1
CDS	590..3940
Translation	MLSATLGANGGSQPLSPSAQATLSKHLPRLQSKRLLQQTQLQSPAAARPQTCDASCLTELCSSENLPEVEIFSLLEEQIPKYKVRNDFLTNFSGYANEDWFVPAPALPIPPEGLGLTKEQTRECLNYFLLCGNRVSQMTRAYDDIEAVTRLLEEKEKDLELTVQIGKELLTQNNALEARVADLETDLKASNDDRAQLVHELHKKNELISVLTNDADDGTDTDTPTMSKSITLDLLQRKVNSLLDENKSLKCEATQLAHQTDEVEEHERQLMADISAQLNDANSQYDNLSLELERQREENRLQHEQIVNLTARLAEAEMRLHQLTQDNDEHLSLLHVTKENQNALALELVEFKQRYEEVLALLHSAQDQLKQQRKRSQPQARSSFLGGLGTSGAGMGGSLFPPDSLHCELMESSLYSENSLDSGISGDSQRSADRISRMMMHMPSGGMSSSTMGGSVYAGAGNVPPYKRVFDTVRCAGKSGNYMDSGNVSMTQLGAMSMSSSSGPRMASMAYPAGSYYRGGSNQSLGVKTLSSESLNSQSDDGYPAQPSGVPGAPGAKELEAALKRLTPAEVLARRAMLSYAPAGTYNYDEPMGHGTGNVRNSDLPLGVRTPDSIMSTGSSGMSGSTNHMSASMTHQWRLPEKLQIIKPMEGSQTLHHWSRLATPTLSGLLDERPGVTIRGGRGLDDLGMQIYTLSDVEEDVSDDLPGKQFEAPGCTFTYTNSMVMHPDDGFVNDLSFLSQSQMSSRMASTSTSRQPSCPATPRAGLSRKNSCSTFSVNLGLAGMLNERGIKAVTPSALNTPAGPNFSPTVTPCNSPEGSPPRAQSPEPLFGLLSCGADLIRRKIVGDQHQQQQQKQRSSLSKQQQQKIMLSHLERRALRSLNLIEKVESIGLENIISAQRGLGSGIANRSSSPLSSGSLQSLHTSSNSIVDDIHFDRAQIKGVLHRGLKSPTPATASTSAAAAASGPGISTSSSTSAYNSDDSDDQGLVMKIKPSKSATPTTTGAAQSQPSSATTSNGTASETRLKQMQRQKSRRQLKNGMANQRPDLGTISGAGGGGRVRPDLGKVADSGSSSKLSTKRSEAKPAEEEEATPQTITQAFVGSVSSLLFGRKGGWL

Target ORF information:

RefSeq Version	NM_164736.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster milton (milt), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164736.3

ORF Insert Sequence:

ATGCTATCCG CAACTTTGGG GGCCAATGGC GGCTCTCAGC CGCTCAGTCC CTCCGCCCAG 
GCGACGCTCA GCAAGCACCT GCCGCGCCTC CAGTCGAAGC GTCTTCTCCA GCAGACGCAG 
TTGCAGTCGC CGGCTGCCGC CAGACCACAG ACCTGCGATG CCAGCTGCCT GACGGAGCTG 
TGCAGCAGCG AGAATTTGCC CGAGGTGGAG ATTTTCTCAC TGCTGGAGGA GCAGATACCC 
AAGTACAAGG TGCGCAACGA TTTCCTCACA AACTTCTCGG GCTATGCGAA TGAGGACTGG 
TTCGTTCCGG CACCAGCGCT GCCCATTCCG CCGGAGGGTC TGGGCTTGAC CAAGGAGCAG 
ACAAGAGAGT GCCTAAACTA TTTCCTTCTA TGCGGCAACC GCGTTAGCCA AATGACTCGG 
GCGTACGATG ACATTGAGGC CGTGACGCGA CTGCTGGAGG AGAAGGAGAA GGACCTAGAG 
CTGACCGTGC AGATTGGCAA AGAGCTGCTC ACCCAGAACA ATGCACTCGA GGCACGGGTC 
GCGGATCTGG AAACCGATCT CAAGGCCTCC AACGACGATC GTGCCCAGCT GGTCCACGAG 
CTGCACAAGA AGAACGAGCT CATCTCTGTG CTTACCAACG ATGCAGACGA TGGCACAGAT 
ACTGACACTC CCACCATGTC GAAGTCTATA ACTTTGGATC TACTGCAGCG CAAGGTCAAC 
TCCCTGCTCG ACGAGAACAA GTCTCTGAAA TGCGAAGCCA CCCAACTGGC CCACCAGACG 
GACGAGGTGG AGGAGCACGA GCGCCAGCTG ATGGCCGATA TCAGTGCTCA GCTAAACGAT 
GCTAACTCAC AGTACGACAA CCTCAGTTTG GAGTTGGAAC GGCAGCGGGA GGAAAACCGG 
CTGCAACATG AGCAGATCGT GAACCTGACC GCTCGCCTAG CGGAGGCGGA AATGCGACTA 
CACCAACTGA CGCAAGACAA CGACGAACAT CTCTCGCTGC TGCACGTGAC GAAAGAAAAC 
CAAAATGCTT TGGCCCTGGA ACTGGTGGAG TTCAAGCAGC GTTACGAAGA GGTGCTCGCT 
CTGCTGCACT CTGCTCAGGA TCAGCTGAAG CAACAGCGAA AGCGGTCGCA GCCACAGGCA 
AGGAGCTCCT TCCTCGGTGG CCTTGGAACA AGTGGCGCTG GCATGGGCGG CAGTCTTTTC 
CCGCCGGATT CACTGCACTG TGAGCTTATG GAGTCTTCAC TGTACTCAGA GAATAGCCTA 
GACTCTGGCA TATCCGGCGA CAGTCAGCGA TCAGCGGACC GCATTAGCCG CATGATGATG 
CACATGCCGT CCGGCGGCAT GAGCTCGTCG ACAATGGGAG GTAGCGTGTA TGCGGGAGCT 
GGCAATGTGC CGCCATACAA GCGGGTATTC GACACAGTGC GATGTGCCGG CAAGAGCGGC 
AACTACATGG ACAGCGGCAA CGTGTCGATG ACGCAACTGG GAGCTATGTC GATGAGCAGC 
TCTTCGGGGC CGCGCATGGC TTCGATGGCG TATCCAGCGG GCTCCTACTA TCGTGGCGGT 
AGCAATCAAT CTCTGGGAGT TAAAACTCTG TCAAGCGAGA GTCTCAACTC CCAGTCGGAT 
GACGGTTATC CAGCCCAGCC CTCTGGTGTG CCAGGAGCGC CCGGCGCCAA GGAGCTGGAG 
GCGGCTCTCA AGAGGCTGAC GCCGGCTGAG GTCTTGGCTC GCCGTGCTAT GTTGTCCTAT 
GCTCCGGCTG GAACCTATAA CTACGACGAA CCAATGGGTC ACGGTACAGG TAACGTTCGA 
AACTCGGATC TACCGCTGGG TGTGCGCACG CCGGACAGTA TTATGTCCAC CGGCTCGTCG 
GGAATGTCGG GCTCCACGAA TCACATGTCC GCTTCGATGA CGCATCAGTG GCGACTACCC 
GAGAAGCTGC AGATCATCAA ACCCATGGAG GGATCGCAGA CATTGCACCA TTGGTCGCGC 
TTGGCCACGC CCACGCTCAG TGGACTGCTG GATGAGCGTC CCGGCGTAAC GATCCGTGGT 
GGACGTGGCC TGGATGATCT GGGAATGCAG ATATACACGC TGTCGGACGT AGAGGAGGAT 
GTTAGCGATG ATCTGCCCGG CAAACAGTTT GAAGCACCGG GCTGCACGTT CACCTACACC 
AATAGCATGG TCATGCATCC GGACGATGGA TTCGTTAACG ATCTGTCATT CCTCTCGCAG 
TCGCAGATGT CGTCCCGAAT GGCCTCCACG TCGACATCAA GGCAACCCAG TTGTCCTGCC 
ACGCCTCGCG CTGGACTATC GCGCAAAAAT TCCTGCTCCA CATTTTCGGT GAACCTCGGA 
CTCGCTGGAA TGCTGAATGA GAGGGGCATC AAGGCGGTGA CGCCCAGTGC CCTCAACACG 
CCCGCCGGAC CTAACTTTTC GCCCACGGTG ACGCCATGCA ACAGCCCGGA GGGATCACCT 
CCTCGTGCCC AGTCGCCCGA GCCGCTCTTT GGTCTGCTCT CGTGTGGTGC GGATTTGATC 
CGGCGCAAAA TCGTAGGCGA TCAGCATCAG CAGCAGCAAC AGAAGCAAAG GAGCAGCCTT 
AGCAAGCAGC AGCAGCAGAA GATCATGCTC TCGCACCTGG AAAGACGTGC CCTGCGATCG 
CTGAACCTAA TTGAGAAGGT GGAGAGCATC GGACTGGAGA ACATAATAAG CGCACAGAGA 
GGCCTTGGCT CAGGAATCGC GAACCGCAGC AGTTCGCCCC TGAGCAGTGG CAGCCTACAG 
AGTCTCCACA CCAGCAGCAA CAGCATTGTG GACGACATAC ACTTCGATCG GGCACAGATC 
AAGGGTGTCC TGCATCGAGG CCTGAAATCG CCAACGCCCG CAACAGCATC AACATCTGCA 
GCTGCGGCGG CTTCAGGGCC AGGTATCTCG ACGAGCAGCA GCACGAGTGC TTACAATAGC 
GATGATAGCG ACGATCAGGG CTTGGTAATG AAAATCAAAC CGTCGAAGAG CGCGACACCC 
ACAACAACAG GAGCAGCACA AAGTCAGCCA AGTTCGGCGA CGACATCCAA CGGCACGGCG 
AGTGAAACGC GACTGAAGCA GATGCAGCGC CAGAAATCGC GCAGGCAGCT AAAGAACGGA 
ATGGCCAACC AGAGGCCAGA TCTGGGCACA ATTTCTGGCG CCGGAGGAGG CGGACGAGTG 
CGCCCGGATC TGGGAAAGGT AGCCGACAGC GGCAGCAGCA GCAAGCTCAG CACCAAGCGA 
AGTGAGGCTA AGCCTGCGGA GGAGGAAGAG GCGACACCAC AGACCATCAC ACAGGCGTTT 
GTGGGTTCAG TTAGTTCCTT GCTTTTTGGC CGCAAGGGCG GTTGGCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

milt ( NM_164738.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723251.1 Drosophila melanogaster milton (milt), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15225
Clone ID Related Accession (Same CDS sequence)	NM_164738.3
Accession Version	NM_164738.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	milton, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164738.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCACG TAAACAATGG GGAAGTAATG GAAAAGGAGA TGGAGCCAAC GGGAGAGCGA 
GAGAGAGACA GGGAGACTGC TGCCGGCAGC GGGGTCCATC ATCGATTTTT AGCCAGTGCA 
TCGGAGCGAG ATGCCAATAG CGGGAATGCA TTGGAAGCTG CAGCGACAAA AACAAAAACA 
GGCACAACGA TTACCAACTT GGAAGACTTG GCCTTTGAGG CATGCCAAAA TTGGTCGGAT 
TTGCACCAAG ACTTCTTCAT CACGGACGAT GAACTTGAGT ACGAGGATGA ACTCTCTTTG 
GGCAGCAGCA TAGGTGGAAA CATTGCAACA ACAACGACAG CAGATGCAGC AGTAGAGGGT 
CTTATAACGG GAGAACATCA GAACGAGCAG CTGCTGATGG AAGTTCTATG CGGCAACCGC 
GTTAGCCAAA TGACTCGGGC GTACGATGAC ATTGAGGCCG TGACGCGACT GCTGGAGGAG 
AAGGAGAAGG ACCTAGAGCT GACCGTGCAG ATTGGCAAAG AGCTGCTCAC CCAGAACAAT 
GCACTCGAGG CACGGGTCGC GGATCTGGAA ACCGATCTCA AGGCCTCCAA CGACGATCGT 
GCCCAGCTGG TCCACGAGCT GCACAAGAAG AACGAGCTCA TCTCTGTGCT TACCAACGAT 
GCAGACGATG GCACAGATAC TGACACTCCC ACCATGTCGA AGTCTATAAC TTTGGATCTA 
CTGCAGCGCA AGGTCAACTC CCTGCTCGAC GAGAACAAGT CTCTGAAATG CGAAGCCACC 
CAACTGGCCC ACCAGACGGA CGAGGTGGAG GAGCACGAGC GCCAGCTGAT GGCCGATATC 
AGTGCTCAGC TAAACGATGC TAACTCACAG TACGACAACC TCAGTTTGGA GTTGGAACGG 
CAGCGGGAGG AAAACCGGCT GCAACATGAG CAGATCGTGA ACCTGACCGC TCGCCTAGCG 
GAGGCGGAAA TGCGACTACA CCAACTGACG CAAGACAACG ACGAACATCT CTCGCTGCTG 
CACGTGACGA AAGAAAACCA AAATGCTTTG GCCCTGGAAC TGGTGGAGTT CAAGCAGCGT 
TACGAAGAGG TGCTCGCTCT GCTGCACTCT GCTCAGGATC AGCTGAAGCA ACAGCGAAAG 
CGGTCGCAGC CACAGGCAAG GAGCTCCTTC CTCGGTGGCC TTGGAACAAG TGGCGCTGGC 
ATGGGCGGCA GTCTTTTCCC GCCGGATTCA CTGCACTGTG AGCTTATGGA GTCTTCACTG 
TACTCAGAGA ATAGCCTAGA CTCTGGCATA TCCGGCGACA GTCAGCGATC AGCGGACCGC 
ATTAGCCGCA TGATGATGCA CATGCCGTCC GGCGGCATGA GCTCGTCGAC AATGGGAGGT 
AGCGTGTATG CGGGAGCTGG CAATGTGCCG CCATACAAGC GGGTATTCGA CACAGTGCGA 
TGTGCCGGCA AGAGCGGCAA CTACATGGAC AGCGGCAACG TGTCGATGAC GCAACTGGGA 
GCTATGTCGA TGAGCAGCTC TTCGGGGCCG CGCATGGCTT CGATGGCGTA TCCAGCGGGC 
TCCTACTATC GTGGCGGTAG CAATCAATCT CTGGGAGTTA AAACTCTGTC AAGCGAGAGT 
CTCAACTCCC AGTCGGATGA CGGTTATCCA GCCCAGCCCT CTGGTGTGCC AGGAGCGCCC 
GGCGCCAAGG AGCTGGAGGC GGCTCTCAAG AGGCTGACGC CGGCTGAGGT CTTGGCTCGC 
CGTGCTATGT TGTCCTATGC TCCGGCTGGA ACCTATAACT ACGACGAACC AATGGGTCAC 
GGTACAGGTA ACGTTCGAAA CTCGGATCTA CCGCTGGGTG TGCGCACGCC GGACAGTATT 
ATGTCCACCG GCTCGTCGGG AATGTCGGGC TCCACGAATC ACATGTCCGC TTCGATGACG 
CATCAGTGGC GACTACCCGA GAAGCTGCAG ATCATCAAAC CCATGGAGGG ATCGCAGACA 
TTGCACCATT GGTCGCGCTT GGCCACGCCC ACGCTCAGTG GACTGCTGGA TGAGCGTCCC 
GGCGTAACGA TCCGTGGTGG ACGTGGCCTG GATGATCTGG GAATGCAGAT ATACACGCTG 
TCGGACGTAG AGGAGGATGT TAGCGATGAT CTGCCCGGCA AACAGTTTGA AGCACCGGGC 
TGCACGTTCA CCTACACCAA TAGCATGGTC ATGCATCCGG ACGATGGATT CGTTAACGAT 
CTGTCATTCC TCTCGCAGTC GCAGATGTCG TCCCGAATGG CCTCCACGTC GACATCAAGG 
CAACCCAGTT GTCCTGCCAC GCCTCGCGCT GGACTATCGC GCAAAAATTC CTGCTCCACA 
TTTTCGGTGA ACCTCGGACT CGCTGGAATG CTGAATGAGA GGGGCATCAA GGCGGTGACG 
CCCAGTGCCC TCAACACGCC CGCCGGACCT AACTTTTCGC CCACGGTGAC GCCATGCAAC 
AGCCCGGAGG GATCACCTCC TCGTGCCCAG TCGCCCGAGC CGCTCTTTGG TCTGCTCTCG 
TGTGGTGCGG ATTTGATCCG GCGCAAAATC GTAGGCGATC AGCATCAGCA GCAGCAACAG 
AAGCAAAGGA GCAGCCTTAG CAAGCAGCAG CAGCAGAAGA TCATGCTCTC GCACCTGGAA 
AGACGTGCCC TGCGATCGCT GAACCTAATT GAGAAGGTGG AGAGCATCGG ACTGGAGAAC 
ATAATAAGCG CACAGAGAGG CCTTGGCTCA GGAATCGCGA ACCGCAGCAG TTCGCCCCTG 
AGCAGTGGCA GCCTACAGAG TCTCCACACC AGCAGCAACA GCATTGTGGA CGACATACAC 
TTCGATCGGG CACAGATCAA GGGTGTCCTG CATCGAGGCC TGAAATCGCC AACGCCCGCA 
ACAGCATCAA CATCTGCAGC TGCGGCGGCT TCAGGGCCAG GTATCTCGAC GAGCAGCAGC 
ACGAGTGCTT ACAATAGCGA TGATAGCGAC GATCAGGGCT TGGTAATGAA AATCAAACCG 
TCGAAGAGCG CGACACCCAC AACAACAGGA GCAGCACAAA GTCAGCCAAG TTCGGCGACG 
ACATCCAACG GCACGGCGAG TGAAACGCGA CTGAAGCAGA TGCAGCGCCA GAAATCGCGC 
AGGCAGCTAA AGAACGGAAT GGCCAACCAG AGGCCAGATC TGGGCACAAT TTCTGGCGCC 
GGAGGAGGCG GACGAGTGCG CCCGGATCTG GGAAAGGTAG CCGACAGCGG CAGCAGCAGC 
AAGCTCAGCA CCAAGCGAAG TGAGGCTAAG CCTGCGGAGG AGGAAGAGGC GACACCACAG 
ACCATCACAC AGGCGTTTGT GGGTTCAGTT AGTTCCTTGC TTTTTGGCCG CAAGGGCGGT 
TGGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723251.1
CDS	214..3582
Translation	MTHVNNGEVMEKEMEPTGERERDRETAAGSGVHHRFLASASERDANSGNALEAAATKTKTGTTITNLEDLAFEACQNWSDLHQDFFITDDELEYEDELSLGSSIGGNIATTTTADAAVEGLITGEHQNEQLLMEVLCGNRVSQMTRAYDDIEAVTRLLEEKEKDLELTVQIGKELLTQNNALEARVADLETDLKASNDDRAQLVHELHKKNELISVLTNDADDGTDTDTPTMSKSITLDLLQRKVNSLLDENKSLKCEATQLAHQTDEVEEHERQLMADISAQLNDANSQYDNLSLELERQREENRLQHEQIVNLTARLAEAEMRLHQLTQDNDEHLSLLHVTKENQNALALELVEFKQRYEEVLALLHSAQDQLKQQRKRSQPQARSSFLGGLGTSGAGMGGSLFPPDSLHCELMESSLYSENSLDSGISGDSQRSADRISRMMMHMPSGGMSSSTMGGSVYAGAGNVPPYKRVFDTVRCAGKSGNYMDSGNVSMTQLGAMSMSSSSGPRMASMAYPAGSYYRGGSNQSLGVKTLSSESLNSQSDDGYPAQPSGVPGAPGAKELEAALKRLTPAEVLARRAMLSYAPAGTYNYDEPMGHGTGNVRNSDLPLGVRTPDSIMSTGSSGMSGSTNHMSASMTHQWRLPEKLQIIKPMEGSQTLHHWSRLATPTLSGLLDERPGVTIRGGRGLDDLGMQIYTLSDVEEDVSDDLPGKQFEAPGCTFTYTNSMVMHPDDGFVNDLSFLSQSQMSSRMASTSTSRQPSCPATPRAGLSRKNSCSTFSVNLGLAGMLNERGIKAVTPSALNTPAGPNFSPTVTPCNSPEGSPPRAQSPEPLFGLLSCGADLIRRKIVGDQHQQQQQKQRSSLSKQQQQKIMLSHLERRALRSLNLIEKVESIGLENIISAQRGLGSGIANRSSSPLSSGSLQSLHTSSNSIVDDIHFDRAQIKGVLHRGLKSPTPATASTSAAAAASGPGISTSSSTSAYNSDDSDDQGLVMKIKPSKSATPTTTGAAQSQPSSATTSNGTASETRLKQMQRQKSRRQLKNGMANQRPDLGTISGAGGGGRVRPDLGKVADSGSSSKLSTKRSEAKPAEEEEATPQTITQAFVGSVSSLLFGRKGGWL

Target ORF information:

RefSeq Version	NM_164738.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster milton (milt), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164738.3

ORF Insert Sequence:

ATGACGCACG TAAACAATGG GGAAGTAATG GAAAAGGAGA TGGAGCCAAC GGGAGAGCGA 
GAGAGAGACA GGGAGACTGC TGCCGGCAGC GGGGTCCATC ATCGATTTTT AGCCAGTGCA 
TCGGAGCGAG ATGCCAATAG CGGGAATGCA TTGGAAGCTG CAGCGACAAA AACAAAAACA 
GGCACAACGA TTACCAACTT GGAAGACTTG GCCTTTGAGG CATGCCAAAA TTGGTCGGAT 
TTGCACCAAG ACTTCTTCAT CACGGACGAT GAACTTGAGT ACGAGGATGA ACTCTCTTTG 
GGCAGCAGCA TAGGTGGAAA CATTGCAACA ACAACGACAG CAGATGCAGC AGTAGAGGGT 
CTTATAACGG GAGAACATCA GAACGAGCAG CTGCTGATGG AAGTTCTATG CGGCAACCGC 
GTTAGCCAAA TGACTCGGGC GTACGATGAC ATTGAGGCCG TGACGCGACT GCTGGAGGAG 
AAGGAGAAGG ACCTAGAGCT GACCGTGCAG ATTGGCAAAG AGCTGCTCAC CCAGAACAAT 
GCACTCGAGG CACGGGTCGC GGATCTGGAA ACCGATCTCA AGGCCTCCAA CGACGATCGT 
GCCCAGCTGG TCCACGAGCT GCACAAGAAG AACGAGCTCA TCTCTGTGCT TACCAACGAT 
GCAGACGATG GCACAGATAC TGACACTCCC ACCATGTCGA AGTCTATAAC TTTGGATCTA 
CTGCAGCGCA AGGTCAACTC CCTGCTCGAC GAGAACAAGT CTCTGAAATG CGAAGCCACC 
CAACTGGCCC ACCAGACGGA CGAGGTGGAG GAGCACGAGC GCCAGCTGAT GGCCGATATC 
AGTGCTCAGC TAAACGATGC TAACTCACAG TACGACAACC TCAGTTTGGA GTTGGAACGG 
CAGCGGGAGG AAAACCGGCT GCAACATGAG CAGATCGTGA ACCTGACCGC TCGCCTAGCG 
GAGGCGGAAA TGCGACTACA CCAACTGACG CAAGACAACG ACGAACATCT CTCGCTGCTG 
CACGTGACGA AAGAAAACCA AAATGCTTTG GCCCTGGAAC TGGTGGAGTT CAAGCAGCGT 
TACGAAGAGG TGCTCGCTCT GCTGCACTCT GCTCAGGATC AGCTGAAGCA ACAGCGAAAG 
CGGTCGCAGC CACAGGCAAG GAGCTCCTTC CTCGGTGGCC TTGGAACAAG TGGCGCTGGC 
ATGGGCGGCA GTCTTTTCCC GCCGGATTCA CTGCACTGTG AGCTTATGGA GTCTTCACTG 
TACTCAGAGA ATAGCCTAGA CTCTGGCATA TCCGGCGACA GTCAGCGATC AGCGGACCGC 
ATTAGCCGCA TGATGATGCA CATGCCGTCC GGCGGCATGA GCTCGTCGAC AATGGGAGGT 
AGCGTGTATG CGGGAGCTGG CAATGTGCCG CCATACAAGC GGGTATTCGA CACAGTGCGA 
TGTGCCGGCA AGAGCGGCAA CTACATGGAC AGCGGCAACG TGTCGATGAC GCAACTGGGA 
GCTATGTCGA TGAGCAGCTC TTCGGGGCCG CGCATGGCTT CGATGGCGTA TCCAGCGGGC 
TCCTACTATC GTGGCGGTAG CAATCAATCT CTGGGAGTTA AAACTCTGTC AAGCGAGAGT 
CTCAACTCCC AGTCGGATGA CGGTTATCCA GCCCAGCCCT CTGGTGTGCC AGGAGCGCCC 
GGCGCCAAGG AGCTGGAGGC GGCTCTCAAG AGGCTGACGC CGGCTGAGGT CTTGGCTCGC 
CGTGCTATGT TGTCCTATGC TCCGGCTGGA ACCTATAACT ACGACGAACC AATGGGTCAC 
GGTACAGGTA ACGTTCGAAA CTCGGATCTA CCGCTGGGTG TGCGCACGCC GGACAGTATT 
ATGTCCACCG GCTCGTCGGG AATGTCGGGC TCCACGAATC ACATGTCCGC TTCGATGACG 
CATCAGTGGC GACTACCCGA GAAGCTGCAG ATCATCAAAC CCATGGAGGG ATCGCAGACA 
TTGCACCATT GGTCGCGCTT GGCCACGCCC ACGCTCAGTG GACTGCTGGA TGAGCGTCCC 
GGCGTAACGA TCCGTGGTGG ACGTGGCCTG GATGATCTGG GAATGCAGAT ATACACGCTG 
TCGGACGTAG AGGAGGATGT TAGCGATGAT CTGCCCGGCA AACAGTTTGA AGCACCGGGC 
TGCACGTTCA CCTACACCAA TAGCATGGTC ATGCATCCGG ACGATGGATT CGTTAACGAT 
CTGTCATTCC TCTCGCAGTC GCAGATGTCG TCCCGAATGG CCTCCACGTC GACATCAAGG 
CAACCCAGTT GTCCTGCCAC GCCTCGCGCT GGACTATCGC GCAAAAATTC CTGCTCCACA 
TTTTCGGTGA ACCTCGGACT CGCTGGAATG CTGAATGAGA GGGGCATCAA GGCGGTGACG 
CCCAGTGCCC TCAACACGCC CGCCGGACCT AACTTTTCGC CCACGGTGAC GCCATGCAAC 
AGCCCGGAGG GATCACCTCC TCGTGCCCAG TCGCCCGAGC CGCTCTTTGG TCTGCTCTCG 
TGTGGTGCGG ATTTGATCCG GCGCAAAATC GTAGGCGATC AGCATCAGCA GCAGCAACAG 
AAGCAAAGGA GCAGCCTTAG CAAGCAGCAG CAGCAGAAGA TCATGCTCTC GCACCTGGAA 
AGACGTGCCC TGCGATCGCT GAACCTAATT GAGAAGGTGG AGAGCATCGG ACTGGAGAAC 
ATAATAAGCG CACAGAGAGG CCTTGGCTCA GGAATCGCGA ACCGCAGCAG TTCGCCCCTG 
AGCAGTGGCA GCCTACAGAG TCTCCACACC AGCAGCAACA GCATTGTGGA CGACATACAC 
TTCGATCGGG CACAGATCAA GGGTGTCCTG CATCGAGGCC TGAAATCGCC AACGCCCGCA 
ACAGCATCAA CATCTGCAGC TGCGGCGGCT TCAGGGCCAG GTATCTCGAC GAGCAGCAGC 
ACGAGTGCTT ACAATAGCGA TGATAGCGAC GATCAGGGCT TGGTAATGAA AATCAAACCG 
TCGAAGAGCG CGACACCCAC AACAACAGGA GCAGCACAAA GTCAGCCAAG TTCGGCGACG 
ACATCCAACG GCACGGCGAG TGAAACGCGA CTGAAGCAGA TGCAGCGCCA GAAATCGCGC 
AGGCAGCTAA AGAACGGAAT GGCCAACCAG AGGCCAGATC TGGGCACAAT TTCTGGCGCC 
GGAGGAGGCG GACGAGTGCG CCCGGATCTG GGAAAGGTAG CCGACAGCGG CAGCAGCAGC 
AAGCTCAGCA CCAAGCGAAG TGAGGCTAAG CCTGCGGAGG AGGAAGAGGC GACACCACAG 
ACCATCACAC AGGCGTTTGT GGGTTCAGTT AGTTCCTTGC TTTTTGGCCG CAAGGGCGGT 
TGGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

milt ( NM_001258998.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245927.1 Drosophila melanogaster milton (milt), transcript variant D, mRNA.

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CloneID	OFa16853
Clone ID Related Accession (Same CDS sequence)	NM_001258998.3 , NM_001259000.3
Accession Version	NM_001258998.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	milton, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001258998.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCATTCC TCAACAGTGC AACGCGTAGT AAACCAAATC ATCAAGAACC AGAGTCCCAT 
CATCATGACA TCAAGAAGCT AGACTCCCTG CCCATAATAG TCGAGCAGGA GGCGGACGAT 
TATGGGCAAA CCTTCGAGCA TGGCAACCAG CTGAATAGCA CCTTTTCCTT GGACGTCGAA 
ACCGGCAGTT CGTCAACCTC GCTGATCGAG AGCAAGTCGA GCAGCTCGCT GTGGTCCACC 
GCCAACTCCG AGGATTCCCT GAACGCCACC CTGATCACCA GCCAACCGTC CAATTCCAGC 
AAGCATTTCG AGAACACCTA CAAGCTGCTG GGTCAGCACC TGGATTTCAA TTGCCAGCGC 
TCGGCGGATA AGCTGCAGCA TCTGAATGGA TCTGCTAGCG ATTTCGATAG CCTGTCCAGC 
TGCTCCTCCA TGTCGGCGAT CGTCAATGGG ATTGAGCCGC CGCCCACGCG TCTGGAACTC 
GAACTGGAGG CAGTGGATCG CATGCGTTGC ATTGTCCAGC AAATGAAGTC GGGGCCAAAG 
TCGATGGATC CGCCGCTGGG CGTGTCGGCT CCTCATCTGG CGCTGCTGCA CGACTTCGCC 
TCGAAGGGAC AACGAAAAGT GGAGGCCAGT GTGCCGGGCA CACCGGTACC CAGTCCGATA 
ACGGGCGCAC TGGAGGCGCC GCACTTTGAG TTGCCACAGG AGCTGCTGCA GGCGGCAAGC 
AGTTGGGAAG TTATGTACTA TGCCTCCAAG AACGTCGATG GCAAAAATTG CCTGAAGGTG 
GTGCGCAGTC TGCTGACGAA CAAAGTTCTA TGCGGCAACC GCGTTAGCCA AATGACTCGG 
GCGTACGATG ACATTGAGGC CGTGACGCGA CTGCTGGAGG AGAAGGAGAA GGACCTAGAG 
CTGACCGTGC AGATTGGCAA AGAGCTGCTC ACCCAGAACA ATGCACTCGA GGCACGGGTC 
GCGGATCTGG AAACCGATCT CAAGGCCTCC AACGACGATC GTGCCCAGCT GGTCCACGAG 
CTGCACAAGA AGAACGAGCT CATCTCTGTG CTTACCAACG ATGCAGACGA TGGCACAGAT 
ACTGACACTC CCACCATGTC GAAGTCTATA ACTTTGGATC TACTGCAGCG CAAGGTCAAC 
TCCCTGCTCG ACGAGAACAA GTCTCTGAAA TGCGAAGCCA CCCAACTGGC CCACCAGACG 
GACGAGGTGG AGGAGCACGA GCGCCAGCTG ATGGCCGATA TCAGTGCTCA GCTAAACGAT 
GCTAACTCAC AGTACGACAA CCTCAGTTTG GAGTTGGAAC GGCAGCGGGA GGAAAACCGG 
CTGCAACATG AGCAGATCGT GAACCTGACC GCTCGCCTAG CGGAGGCGGA AATGCGACTA 
CACCAACTGA CGCAAGACAA CGACGAACAT CTCTCGCTGC TGCACGTGAC GAAAGAAAAC 
CAAAATGCTT TGGCCCTGGA ACTGGTGGAG TTCAAGCAGC GTTACGAAGA GGTGCTCGCT 
CTGCTGCACT CTGCTCAGGA TCAGCTGAAG CAACAGCGAA AGCGGTCGCA GCCACAGGCA 
AGGAGCTCCT TCCTCGGTGG CCTTGGAACA AGTGGCGCTG GCATGGGCGG CAGTCTTTTC 
CCGCCGGATT CACTGCACTG TGAGCTTATG GAGTCTTCAC TGTACTCAGA GAATAGCCTA 
GACTCTGGCA TATCCGGCGA CAGTCAGCGA TCAGCGGACC GCATTAGCCG CATGATGATG 
CACATGCCGT CCGGCGGCAT GAGCTCGTCG ACAATGGGAG GTAGCGTGTA TGCGGGAGCT 
GGCAATGTGC CGCCATACAA GCGGGTATTC GACACAGTGC GATGTGCCGG CAAGAGCGGC 
AACTACATGG ACAGCGGCAA CGTGTCGATG ACGCAACTGG GAGCTATGTC GATGAGCAGC 
TCTTCGGGGC CGCGCATGGC TTCGATGGCG TATCCAGCGG GCTCCTACTA TCGTGGCGGT 
AGCAATCAAT CTCTGGGAGT TAAAACTCTG TCAAGCGAGA GTCTCAACTC CCAGTCGGAT 
GACGGTTATC CAGCCCAGCC CTCTGGTGTG CCAGGAGCGC CCGGCGCCAA GGAGCTGGAG 
GCGGCTCTCA AGAGGCTGAC GCCGGCTGAG GTCTTGGCTC GCCGTGCTAT GTTGTCCTAT 
GCTCCGGCTG GAACCTATAA CTACGACGAA CCAATGGGTC ACGGTACAGG TAACGTTCGA 
AACTCGGATC TACCGCTGGG TGTGCGCACG CCGGACAGTA TTATGTCCAC CGGCTCGTCG 
GGAATGTCGG GCTCCACGAA TCACATGTCC GCTTCGATGA CGCATCAGTG GCGACTACCC 
GAGAAGCTGC AGATCATCAA ACCCATGGAG GGATCGCAGA CATTGCACCA TTGGTCGCGC 
TTGGCCACGC CCACGCTCAG TGGACTGCTG GATGAGCGTC CCGGCGTAAC GATCCGTGGT 
GGACGTGGCC TGGATGATCT GGGAATGCAG ATATACACGC TGTCGGACGT AGAGGAGGAT 
GTTAGCGATG ATCTGCCCGG CAAACAGTTT GAAGCACCGG GCTGCACGTT CACCTACACC 
AATAGCATGG TCATGCATCC GGACGATGGA TTCGTTAACG ATCTGTCATT CCTCTCGCAG 
TCGCAGATGT CGTCCCGAAT GGCCTCCACG TCGACATCAA GGCAACCCAG TTGTCCTGCC 
ACGCCTCGCG CTGGACTATC GCGCAAAAAT TCCTGCTCCA CATTTTCGGT GAACCTCGGA 
CTCGCTGGAA TGCTGAATGA GAGGGGCATC AAGGCGGTGA CGCCCAGTGC CCTCAACACG 
CCCGCCGGAC CTAACTTTTC GCCCACGGTG ACGCCATGCA ACAGCCCGGA GGGATCACCT 
CCTCGTGCCC AGTCGCCCGA GCCGCTCTTT GGTCTGCTCT CGTGTGGTGC GGATTTGATC 
CGGCGCAAAA TCGTAGGCGA TCAGCATCAG CAGCAGCAAC AGAAGCAAAG GAGCAGCCTT 
AGCAAGCAGC AGCAGCAGAA GATCATGCTC TCGCACCTGG AAAGACGTGC CCTGCGATCG 
CTGAACCTAA TTGAGAAGGT GGAGAGCATC GGACTGGAGA ACATAATAAG CGCACAGAGA 
GGCCTTGGCT CAGGAATCGC GAACCGCAGC AGTTCGCCCC TGAGCAGTGG CAGCCTACAG 
AGTCTCCACA CCAGCAGCAA CAGCATTGTG GACGACATAC ACTTCGATCG GGCACAGATC 
AAGGGTGTCC TGCATCGAGG CCTGAAATCG CCAACGCCCG CAACAGCATC AACATCTGCA 
GCTGCGGCGG CTTCAGGGCC AGGTATCTCG ACGAGCAGCA GCACGAGTGC TTACAATAGC 
GATGATAGCG ACGATCAGGG CTTGGTAATG AAAATCAAAC CGTCGAAGAG CGCGACACCC 
ACAACAACAG GAGCAGCACA AAGTCAGCCA AGTTCGGCGA CGACATCCAA CGGCACGGCG 
AGTGAAACGC GACTGAAGCA GATGCAGCGC CAGAAATCGC GCAGGCAGCT AAAGAACGGA 
ATGGCCAACC AGAGGCCAGA TCTGGGCACA ATTTCTGGCG CCGGAGGAGG CGGACGAGTG 
CGCCCGGATC TGGGAAAGGT AGCCGACAGC GGCAGCAGCA GCAAGCTCAG CACCAAGCGA 
AGTGAGGCTA AGCCTGCGGA GGAGGAAGAG GCGACACCAC AGACCATCAC ACAGGCGTTT 
GTGGGTTCAG TTAGTTCCTT GCTTTTTGGC CGCAAGGGCG GTTGGCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245927.1
CDS	311..4081
Translation	MPFLNSATRSKPNHQEPESHHHDIKKLDSLPIIVEQEADDYGQTFEHGNQLNSTFSLDVETGSSSTSLIESKSSSSLWSTANSEDSLNATLITSQPSNSSKHFENTYKLLGQHLDFNCQRSADKLQHLNGSASDFDSLSSCSSMSAIVNGIEPPPTRLELELEAVDRMRCIVQQMKSGPKSMDPPLGVSAPHLALLHDFASKGQRKVEASVPGTPVPSPITGALEAPHFELPQELLQAASSWEVMYYASKNVDGKNCLKVVRSLLTNKVLCGNRVSQMTRAYDDIEAVTRLLEEKEKDLELTVQIGKELLTQNNALEARVADLETDLKASNDDRAQLVHELHKKNELISVLTNDADDGTDTDTPTMSKSITLDLLQRKVNSLLDENKSLKCEATQLAHQTDEVEEHERQLMADISAQLNDANSQYDNLSLELERQREENRLQHEQIVNLTARLAEAEMRLHQLTQDNDEHLSLLHVTKENQNALALELVEFKQRYEEVLALLHSAQDQLKQQRKRSQPQARSSFLGGLGTSGAGMGGSLFPPDSLHCELMESSLYSENSLDSGISGDSQRSADRISRMMMHMPSGGMSSSTMGGSVYAGAGNVPPYKRVFDTVRCAGKSGNYMDSGNVSMTQLGAMSMSSSSGPRMASMAYPAGSYYRGGSNQSLGVKTLSSESLNSQSDDGYPAQPSGVPGAPGAKELEAALKRLTPAEVLARRAMLSYAPAGTYNYDEPMGHGTGNVRNSDLPLGVRTPDSIMSTGSSGMSGSTNHMSASMTHQWRLPEKLQIIKPMEGSQTLHHWSRLATPTLSGLLDERPGVTIRGGRGLDDLGMQIYTLSDVEEDVSDDLPGKQFEAPGCTFTYTNSMVMHPDDGFVNDLSFLSQSQMSSRMASTSTSRQPSCPATPRAGLSRKNSCSTFSVNLGLAGMLNERGIKAVTPSALNTPAGPNFSPTVTPCNSPEGSPPRAQSPEPLFGLLSCGADLIRRKIVGDQHQQQQQKQRSSLSKQQQQKIMLSHLERRALRSLNLIEKVESIGLENIISAQRGLGSGIANRSSSPLSSGSLQSLHTSSNSIVDDIHFDRAQIKGVLHRGLKSPTPATASTSAAAAASGPGISTSSSTSAYNSDDSDDQGLVMKIKPSKSATPTTTGAAQSQPSSATTSNGTASETRLKQMQRQKSRRQLKNGMANQRPDLGTISGAGGGGRVRPDLGKVADSGSSSKLSTKRSEAKPAEEEEATPQTITQAFVGSVSSLLFGRKGGWL

Target ORF information:

RefSeq Version	NM_001258998.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster milton (milt), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258998.3

ORF Insert Sequence:

ATGCCATTCC TCAACAGTGC AACGCGTAGT AAACCAAATC ATCAAGAACC AGAGTCCCAT 
CATCATGACA TCAAGAAGCT AGACTCCCTG CCCATAATAG TCGAGCAGGA GGCGGACGAT 
TATGGGCAAA CCTTCGAGCA TGGCAACCAG CTGAATAGCA CCTTTTCCTT GGACGTCGAA 
ACCGGCAGTT CGTCAACCTC GCTGATCGAG AGCAAGTCGA GCAGCTCGCT GTGGTCCACC 
GCCAACTCCG AGGATTCCCT GAACGCCACC CTGATCACCA GCCAACCGTC CAATTCCAGC 
AAGCATTTCG AGAACACCTA CAAGCTGCTG GGTCAGCACC TGGATTTCAA TTGCCAGCGC 
TCGGCGGATA AGCTGCAGCA TCTGAATGGA TCTGCTAGCG ATTTCGATAG CCTGTCCAGC 
TGCTCCTCCA TGTCGGCGAT CGTCAATGGG ATTGAGCCGC CGCCCACGCG TCTGGAACTC 
GAACTGGAGG CAGTGGATCG CATGCGTTGC ATTGTCCAGC AAATGAAGTC GGGGCCAAAG 
TCGATGGATC CGCCGCTGGG CGTGTCGGCT CCTCATCTGG CGCTGCTGCA CGACTTCGCC 
TCGAAGGGAC AACGAAAAGT GGAGGCCAGT GTGCCGGGCA CACCGGTACC CAGTCCGATA 
ACGGGCGCAC TGGAGGCGCC GCACTTTGAG TTGCCACAGG AGCTGCTGCA GGCGGCAAGC 
AGTTGGGAAG TTATGTACTA TGCCTCCAAG AACGTCGATG GCAAAAATTG CCTGAAGGTG 
GTGCGCAGTC TGCTGACGAA CAAAGTTCTA TGCGGCAACC GCGTTAGCCA AATGACTCGG 
GCGTACGATG ACATTGAGGC CGTGACGCGA CTGCTGGAGG AGAAGGAGAA GGACCTAGAG 
CTGACCGTGC AGATTGGCAA AGAGCTGCTC ACCCAGAACA ATGCACTCGA GGCACGGGTC 
GCGGATCTGG AAACCGATCT CAAGGCCTCC AACGACGATC GTGCCCAGCT GGTCCACGAG 
CTGCACAAGA AGAACGAGCT CATCTCTGTG CTTACCAACG ATGCAGACGA TGGCACAGAT 
ACTGACACTC CCACCATGTC GAAGTCTATA ACTTTGGATC TACTGCAGCG CAAGGTCAAC 
TCCCTGCTCG ACGAGAACAA GTCTCTGAAA TGCGAAGCCA CCCAACTGGC CCACCAGACG 
GACGAGGTGG AGGAGCACGA GCGCCAGCTG ATGGCCGATA TCAGTGCTCA GCTAAACGAT 
GCTAACTCAC AGTACGACAA CCTCAGTTTG GAGTTGGAAC GGCAGCGGGA GGAAAACCGG 
CTGCAACATG AGCAGATCGT GAACCTGACC GCTCGCCTAG CGGAGGCGGA AATGCGACTA 
CACCAACTGA CGCAAGACAA CGACGAACAT CTCTCGCTGC TGCACGTGAC GAAAGAAAAC 
CAAAATGCTT TGGCCCTGGA ACTGGTGGAG TTCAAGCAGC GTTACGAAGA GGTGCTCGCT 
CTGCTGCACT CTGCTCAGGA TCAGCTGAAG CAACAGCGAA AGCGGTCGCA GCCACAGGCA 
AGGAGCTCCT TCCTCGGTGG CCTTGGAACA AGTGGCGCTG GCATGGGCGG CAGTCTTTTC 
CCGCCGGATT CACTGCACTG TGAGCTTATG GAGTCTTCAC TGTACTCAGA GAATAGCCTA 
GACTCTGGCA TATCCGGCGA CAGTCAGCGA TCAGCGGACC GCATTAGCCG CATGATGATG 
CACATGCCGT CCGGCGGCAT GAGCTCGTCG ACAATGGGAG GTAGCGTGTA TGCGGGAGCT 
GGCAATGTGC CGCCATACAA GCGGGTATTC GACACAGTGC GATGTGCCGG CAAGAGCGGC 
AACTACATGG ACAGCGGCAA CGTGTCGATG ACGCAACTGG GAGCTATGTC GATGAGCAGC 
TCTTCGGGGC CGCGCATGGC TTCGATGGCG TATCCAGCGG GCTCCTACTA TCGTGGCGGT 
AGCAATCAAT CTCTGGGAGT TAAAACTCTG TCAAGCGAGA GTCTCAACTC CCAGTCGGAT 
GACGGTTATC CAGCCCAGCC CTCTGGTGTG CCAGGAGCGC CCGGCGCCAA GGAGCTGGAG 
GCGGCTCTCA AGAGGCTGAC GCCGGCTGAG GTCTTGGCTC GCCGTGCTAT GTTGTCCTAT 
GCTCCGGCTG GAACCTATAA CTACGACGAA CCAATGGGTC ACGGTACAGG TAACGTTCGA 
AACTCGGATC TACCGCTGGG TGTGCGCACG CCGGACAGTA TTATGTCCAC CGGCTCGTCG 
GGAATGTCGG GCTCCACGAA TCACATGTCC GCTTCGATGA CGCATCAGTG GCGACTACCC 
GAGAAGCTGC AGATCATCAA ACCCATGGAG GGATCGCAGA CATTGCACCA TTGGTCGCGC 
TTGGCCACGC CCACGCTCAG TGGACTGCTG GATGAGCGTC CCGGCGTAAC GATCCGTGGT 
GGACGTGGCC TGGATGATCT GGGAATGCAG ATATACACGC TGTCGGACGT AGAGGAGGAT 
GTTAGCGATG ATCTGCCCGG CAAACAGTTT GAAGCACCGG GCTGCACGTT CACCTACACC 
AATAGCATGG TCATGCATCC GGACGATGGA TTCGTTAACG ATCTGTCATT CCTCTCGCAG 
TCGCAGATGT CGTCCCGAAT GGCCTCCACG TCGACATCAA GGCAACCCAG TTGTCCTGCC 
ACGCCTCGCG CTGGACTATC GCGCAAAAAT TCCTGCTCCA CATTTTCGGT GAACCTCGGA 
CTCGCTGGAA TGCTGAATGA GAGGGGCATC AAGGCGGTGA CGCCCAGTGC CCTCAACACG 
CCCGCCGGAC CTAACTTTTC GCCCACGGTG ACGCCATGCA ACAGCCCGGA GGGATCACCT 
CCTCGTGCCC AGTCGCCCGA GCCGCTCTTT GGTCTGCTCT CGTGTGGTGC GGATTTGATC 
CGGCGCAAAA TCGTAGGCGA TCAGCATCAG CAGCAGCAAC AGAAGCAAAG GAGCAGCCTT 
AGCAAGCAGC AGCAGCAGAA GATCATGCTC TCGCACCTGG AAAGACGTGC CCTGCGATCG 
CTGAACCTAA TTGAGAAGGT GGAGAGCATC GGACTGGAGA ACATAATAAG CGCACAGAGA 
GGCCTTGGCT CAGGAATCGC GAACCGCAGC AGTTCGCCCC TGAGCAGTGG CAGCCTACAG 
AGTCTCCACA CCAGCAGCAA CAGCATTGTG GACGACATAC ACTTCGATCG GGCACAGATC 
AAGGGTGTCC TGCATCGAGG CCTGAAATCG CCAACGCCCG CAACAGCATC AACATCTGCA 
GCTGCGGCGG CTTCAGGGCC AGGTATCTCG ACGAGCAGCA GCACGAGTGC TTACAATAGC 
GATGATAGCG ACGATCAGGG CTTGGTAATG AAAATCAAAC CGTCGAAGAG CGCGACACCC 
ACAACAACAG GAGCAGCACA AAGTCAGCCA AGTTCGGCGA CGACATCCAA CGGCACGGCG 
AGTGAAACGC GACTGAAGCA GATGCAGCGC CAGAAATCGC GCAGGCAGCT AAAGAACGGA 
ATGGCCAACC AGAGGCCAGA TCTGGGCACA ATTTCTGGCG CCGGAGGAGG CGGACGAGTG 
CGCCCGGATC TGGGAAAGGT AGCCGACAGC GGCAGCAGCA GCAAGCTCAG CACCAAGCGA 
AGTGAGGCTA AGCCTGCGGA GGAGGAAGAG GCGACACCAC AGACCATCAC ACAGGCGTTT 
GTGGGTTCAG TTAGTTCCTT GCTTTTTGGC CGCAAGGGCG GTTGGCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

milt ( NM_001259000.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245929.1 Drosophila melanogaster milton (milt), transcript variant F, mRNA.

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CloneID	OFa16853
Clone ID Related Accession (Same CDS sequence)	NM_001258998.3 , NM_001259000.3
Accession Version	NM_001259000.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	milton, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001259000.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCATTCC TCAACAGTGC AACGCGTAGT AAACCAAATC ATCAAGAACC AGAGTCCCAT 
CATCATGACA TCAAGAAGCT AGACTCCCTG CCCATAATAG TCGAGCAGGA GGCGGACGAT 
TATGGGCAAA CCTTCGAGCA TGGCAACCAG CTGAATAGCA CCTTTTCCTT GGACGTCGAA 
ACCGGCAGTT CGTCAACCTC GCTGATCGAG AGCAAGTCGA GCAGCTCGCT GTGGTCCACC 
GCCAACTCCG AGGATTCCCT GAACGCCACC CTGATCACCA GCCAACCGTC CAATTCCAGC 
AAGCATTTCG AGAACACCTA CAAGCTGCTG GGTCAGCACC TGGATTTCAA TTGCCAGCGC 
TCGGCGGATA AGCTGCAGCA TCTGAATGGA TCTGCTAGCG ATTTCGATAG CCTGTCCAGC 
TGCTCCTCCA TGTCGGCGAT CGTCAATGGG ATTGAGCCGC CGCCCACGCG TCTGGAACTC 
GAACTGGAGG CAGTGGATCG CATGCGTTGC ATTGTCCAGC AAATGAAGTC GGGGCCAAAG 
TCGATGGATC CGCCGCTGGG CGTGTCGGCT CCTCATCTGG CGCTGCTGCA CGACTTCGCC 
TCGAAGGGAC AACGAAAAGT GGAGGCCAGT GTGCCGGGCA CACCGGTACC CAGTCCGATA 
ACGGGCGCAC TGGAGGCGCC GCACTTTGAG TTGCCACAGG AGCTGCTGCA GGCGGCAAGC 
AGTTGGGAAG TTATGTACTA TGCCTCCAAG AACGTCGATG GCAAAAATTG CCTGAAGGTG 
GTGCGCAGTC TGCTGACGAA CAAAGTTCTA TGCGGCAACC GCGTTAGCCA AATGACTCGG 
GCGTACGATG ACATTGAGGC CGTGACGCGA CTGCTGGAGG AGAAGGAGAA GGACCTAGAG 
CTGACCGTGC AGATTGGCAA AGAGCTGCTC ACCCAGAACA ATGCACTCGA GGCACGGGTC 
GCGGATCTGG AAACCGATCT CAAGGCCTCC AACGACGATC GTGCCCAGCT GGTCCACGAG 
CTGCACAAGA AGAACGAGCT CATCTCTGTG CTTACCAACG ATGCAGACGA TGGCACAGAT 
ACTGACACTC CCACCATGTC GAAGTCTATA ACTTTGGATC TACTGCAGCG CAAGGTCAAC 
TCCCTGCTCG ACGAGAACAA GTCTCTGAAA TGCGAAGCCA CCCAACTGGC CCACCAGACG 
GACGAGGTGG AGGAGCACGA GCGCCAGCTG ATGGCCGATA TCAGTGCTCA GCTAAACGAT 
GCTAACTCAC AGTACGACAA CCTCAGTTTG GAGTTGGAAC GGCAGCGGGA GGAAAACCGG 
CTGCAACATG AGCAGATCGT GAACCTGACC GCTCGCCTAG CGGAGGCGGA AATGCGACTA 
CACCAACTGA CGCAAGACAA CGACGAACAT CTCTCGCTGC TGCACGTGAC GAAAGAAAAC 
CAAAATGCTT TGGCCCTGGA ACTGGTGGAG TTCAAGCAGC GTTACGAAGA GGTGCTCGCT 
CTGCTGCACT CTGCTCAGGA TCAGCTGAAG CAACAGCGAA AGCGGTCGCA GCCACAGGCA 
AGGAGCTCCT TCCTCGGTGG CCTTGGAACA AGTGGCGCTG GCATGGGCGG CAGTCTTTTC 
CCGCCGGATT CACTGCACTG TGAGCTTATG GAGTCTTCAC TGTACTCAGA GAATAGCCTA 
GACTCTGGCA TATCCGGCGA CAGTCAGCGA TCAGCGGACC GCATTAGCCG CATGATGATG 
CACATGCCGT CCGGCGGCAT GAGCTCGTCG ACAATGGGAG GTAGCGTGTA TGCGGGAGCT 
GGCAATGTGC CGCCATACAA GCGGGTATTC GACACAGTGC GATGTGCCGG CAAGAGCGGC 
AACTACATGG ACAGCGGCAA CGTGTCGATG ACGCAACTGG GAGCTATGTC GATGAGCAGC 
TCTTCGGGGC CGCGCATGGC TTCGATGGCG TATCCAGCGG GCTCCTACTA TCGTGGCGGT 
AGCAATCAAT CTCTGGGAGT TAAAACTCTG TCAAGCGAGA GTCTCAACTC CCAGTCGGAT 
GACGGTTATC CAGCCCAGCC CTCTGGTGTG CCAGGAGCGC CCGGCGCCAA GGAGCTGGAG 
GCGGCTCTCA AGAGGCTGAC GCCGGCTGAG GTCTTGGCTC GCCGTGCTAT GTTGTCCTAT 
GCTCCGGCTG GAACCTATAA CTACGACGAA CCAATGGGTC ACGGTACAGG TAACGTTCGA 
AACTCGGATC TACCGCTGGG TGTGCGCACG CCGGACAGTA TTATGTCCAC CGGCTCGTCG 
GGAATGTCGG GCTCCACGAA TCACATGTCC GCTTCGATGA CGCATCAGTG GCGACTACCC 
GAGAAGCTGC AGATCATCAA ACCCATGGAG GGATCGCAGA CATTGCACCA TTGGTCGCGC 
TTGGCCACGC CCACGCTCAG TGGACTGCTG GATGAGCGTC CCGGCGTAAC GATCCGTGGT 
GGACGTGGCC TGGATGATCT GGGAATGCAG ATATACACGC TGTCGGACGT AGAGGAGGAT 
GTTAGCGATG ATCTGCCCGG CAAACAGTTT GAAGCACCGG GCTGCACGTT CACCTACACC 
AATAGCATGG TCATGCATCC GGACGATGGA TTCGTTAACG ATCTGTCATT CCTCTCGCAG 
TCGCAGATGT CGTCCCGAAT GGCCTCCACG TCGACATCAA GGCAACCCAG TTGTCCTGCC 
ACGCCTCGCG CTGGACTATC GCGCAAAAAT TCCTGCTCCA CATTTTCGGT GAACCTCGGA 
CTCGCTGGAA TGCTGAATGA GAGGGGCATC AAGGCGGTGA CGCCCAGTGC CCTCAACACG 
CCCGCCGGAC CTAACTTTTC GCCCACGGTG ACGCCATGCA ACAGCCCGGA GGGATCACCT 
CCTCGTGCCC AGTCGCCCGA GCCGCTCTTT GGTCTGCTCT CGTGTGGTGC GGATTTGATC 
CGGCGCAAAA TCGTAGGCGA TCAGCATCAG CAGCAGCAAC AGAAGCAAAG GAGCAGCCTT 
AGCAAGCAGC AGCAGCAGAA GATCATGCTC TCGCACCTGG AAAGACGTGC CCTGCGATCG 
CTGAACCTAA TTGAGAAGGT GGAGAGCATC GGACTGGAGA ACATAATAAG CGCACAGAGA 
GGCCTTGGCT CAGGAATCGC GAACCGCAGC AGTTCGCCCC TGAGCAGTGG CAGCCTACAG 
AGTCTCCACA CCAGCAGCAA CAGCATTGTG GACGACATAC ACTTCGATCG GGCACAGATC 
AAGGGTGTCC TGCATCGAGG CCTGAAATCG CCAACGCCCG CAACAGCATC AACATCTGCA 
GCTGCGGCGG CTTCAGGGCC AGGTATCTCG ACGAGCAGCA GCACGAGTGC TTACAATAGC 
GATGATAGCG ACGATCAGGG CTTGGTAATG AAAATCAAAC CGTCGAAGAG CGCGACACCC 
ACAACAACAG GAGCAGCACA AAGTCAGCCA AGTTCGGCGA CGACATCCAA CGGCACGGCG 
AGTGAAACGC GACTGAAGCA GATGCAGCGC CAGAAATCGC GCAGGCAGCT AAAGAACGGA 
ATGGCCAACC AGAGGCCAGA TCTGGGCACA ATTTCTGGCG CCGGAGGAGG CGGACGAGTG 
CGCCCGGATC TGGGAAAGGT AGCCGACAGC GGCAGCAGCA GCAAGCTCAG CACCAAGCGA 
AGTGAGGCTA AGCCTGCGGA GGAGGAAGAG GCGACACCAC AGACCATCAC ACAGGCGTTT 
GTGGGTTCAG TTAGTTCCTT GCTTTTTGGC CGCAAGGGCG GTTGGCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245929.1
CDS	245..4015
Translation	MPFLNSATRSKPNHQEPESHHHDIKKLDSLPIIVEQEADDYGQTFEHGNQLNSTFSLDVETGSSSTSLIESKSSSSLWSTANSEDSLNATLITSQPSNSSKHFENTYKLLGQHLDFNCQRSADKLQHLNGSASDFDSLSSCSSMSAIVNGIEPPPTRLELELEAVDRMRCIVQQMKSGPKSMDPPLGVSAPHLALLHDFASKGQRKVEASVPGTPVPSPITGALEAPHFELPQELLQAASSWEVMYYASKNVDGKNCLKVVRSLLTNKVLCGNRVSQMTRAYDDIEAVTRLLEEKEKDLELTVQIGKELLTQNNALEARVADLETDLKASNDDRAQLVHELHKKNELISVLTNDADDGTDTDTPTMSKSITLDLLQRKVNSLLDENKSLKCEATQLAHQTDEVEEHERQLMADISAQLNDANSQYDNLSLELERQREENRLQHEQIVNLTARLAEAEMRLHQLTQDNDEHLSLLHVTKENQNALALELVEFKQRYEEVLALLHSAQDQLKQQRKRSQPQARSSFLGGLGTSGAGMGGSLFPPDSLHCELMESSLYSENSLDSGISGDSQRSADRISRMMMHMPSGGMSSSTMGGSVYAGAGNVPPYKRVFDTVRCAGKSGNYMDSGNVSMTQLGAMSMSSSSGPRMASMAYPAGSYYRGGSNQSLGVKTLSSESLNSQSDDGYPAQPSGVPGAPGAKELEAALKRLTPAEVLARRAMLSYAPAGTYNYDEPMGHGTGNVRNSDLPLGVRTPDSIMSTGSSGMSGSTNHMSASMTHQWRLPEKLQIIKPMEGSQTLHHWSRLATPTLSGLLDERPGVTIRGGRGLDDLGMQIYTLSDVEEDVSDDLPGKQFEAPGCTFTYTNSMVMHPDDGFVNDLSFLSQSQMSSRMASTSTSRQPSCPATPRAGLSRKNSCSTFSVNLGLAGMLNERGIKAVTPSALNTPAGPNFSPTVTPCNSPEGSPPRAQSPEPLFGLLSCGADLIRRKIVGDQHQQQQQKQRSSLSKQQQQKIMLSHLERRALRSLNLIEKVESIGLENIISAQRGLGSGIANRSSSPLSSGSLQSLHTSSNSIVDDIHFDRAQIKGVLHRGLKSPTPATASTSAAAAASGPGISTSSSTSAYNSDDSDDQGLVMKIKPSKSATPTTTGAAQSQPSSATTSNGTASETRLKQMQRQKSRRQLKNGMANQRPDLGTISGAGGGGRVRPDLGKVADSGSSSKLSTKRSEAKPAEEEEATPQTITQAFVGSVSSLLFGRKGGWL

Target ORF information:

RefSeq Version	NM_001259000.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster milton (milt), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259000.3

ORF Insert Sequence:

ATGCCATTCC TCAACAGTGC AACGCGTAGT AAACCAAATC ATCAAGAACC AGAGTCCCAT 
CATCATGACA TCAAGAAGCT AGACTCCCTG CCCATAATAG TCGAGCAGGA GGCGGACGAT 
TATGGGCAAA CCTTCGAGCA TGGCAACCAG CTGAATAGCA CCTTTTCCTT GGACGTCGAA 
ACCGGCAGTT CGTCAACCTC GCTGATCGAG AGCAAGTCGA GCAGCTCGCT GTGGTCCACC 
GCCAACTCCG AGGATTCCCT GAACGCCACC CTGATCACCA GCCAACCGTC CAATTCCAGC 
AAGCATTTCG AGAACACCTA CAAGCTGCTG GGTCAGCACC TGGATTTCAA TTGCCAGCGC 
TCGGCGGATA AGCTGCAGCA TCTGAATGGA TCTGCTAGCG ATTTCGATAG CCTGTCCAGC 
TGCTCCTCCA TGTCGGCGAT CGTCAATGGG ATTGAGCCGC CGCCCACGCG TCTGGAACTC 
GAACTGGAGG CAGTGGATCG CATGCGTTGC ATTGTCCAGC AAATGAAGTC GGGGCCAAAG 
TCGATGGATC CGCCGCTGGG CGTGTCGGCT CCTCATCTGG CGCTGCTGCA CGACTTCGCC 
TCGAAGGGAC AACGAAAAGT GGAGGCCAGT GTGCCGGGCA CACCGGTACC CAGTCCGATA 
ACGGGCGCAC TGGAGGCGCC GCACTTTGAG TTGCCACAGG AGCTGCTGCA GGCGGCAAGC 
AGTTGGGAAG TTATGTACTA TGCCTCCAAG AACGTCGATG GCAAAAATTG CCTGAAGGTG 
GTGCGCAGTC TGCTGACGAA CAAAGTTCTA TGCGGCAACC GCGTTAGCCA AATGACTCGG 
GCGTACGATG ACATTGAGGC CGTGACGCGA CTGCTGGAGG AGAAGGAGAA GGACCTAGAG 
CTGACCGTGC AGATTGGCAA AGAGCTGCTC ACCCAGAACA ATGCACTCGA GGCACGGGTC 
GCGGATCTGG AAACCGATCT CAAGGCCTCC AACGACGATC GTGCCCAGCT GGTCCACGAG 
CTGCACAAGA AGAACGAGCT CATCTCTGTG CTTACCAACG ATGCAGACGA TGGCACAGAT 
ACTGACACTC CCACCATGTC GAAGTCTATA ACTTTGGATC TACTGCAGCG CAAGGTCAAC 
TCCCTGCTCG ACGAGAACAA GTCTCTGAAA TGCGAAGCCA CCCAACTGGC CCACCAGACG 
GACGAGGTGG AGGAGCACGA GCGCCAGCTG ATGGCCGATA TCAGTGCTCA GCTAAACGAT 
GCTAACTCAC AGTACGACAA CCTCAGTTTG GAGTTGGAAC GGCAGCGGGA GGAAAACCGG 
CTGCAACATG AGCAGATCGT GAACCTGACC GCTCGCCTAG CGGAGGCGGA AATGCGACTA 
CACCAACTGA CGCAAGACAA CGACGAACAT CTCTCGCTGC TGCACGTGAC GAAAGAAAAC 
CAAAATGCTT TGGCCCTGGA ACTGGTGGAG TTCAAGCAGC GTTACGAAGA GGTGCTCGCT 
CTGCTGCACT CTGCTCAGGA TCAGCTGAAG CAACAGCGAA AGCGGTCGCA GCCACAGGCA 
AGGAGCTCCT TCCTCGGTGG CCTTGGAACA AGTGGCGCTG GCATGGGCGG CAGTCTTTTC 
CCGCCGGATT CACTGCACTG TGAGCTTATG GAGTCTTCAC TGTACTCAGA GAATAGCCTA 
GACTCTGGCA TATCCGGCGA CAGTCAGCGA TCAGCGGACC GCATTAGCCG CATGATGATG 
CACATGCCGT CCGGCGGCAT GAGCTCGTCG ACAATGGGAG GTAGCGTGTA TGCGGGAGCT 
GGCAATGTGC CGCCATACAA GCGGGTATTC GACACAGTGC GATGTGCCGG CAAGAGCGGC 
AACTACATGG ACAGCGGCAA CGTGTCGATG ACGCAACTGG GAGCTATGTC GATGAGCAGC 
TCTTCGGGGC CGCGCATGGC TTCGATGGCG TATCCAGCGG GCTCCTACTA TCGTGGCGGT 
AGCAATCAAT CTCTGGGAGT TAAAACTCTG TCAAGCGAGA GTCTCAACTC CCAGTCGGAT 
GACGGTTATC CAGCCCAGCC CTCTGGTGTG CCAGGAGCGC CCGGCGCCAA GGAGCTGGAG 
GCGGCTCTCA AGAGGCTGAC GCCGGCTGAG GTCTTGGCTC GCCGTGCTAT GTTGTCCTAT 
GCTCCGGCTG GAACCTATAA CTACGACGAA CCAATGGGTC ACGGTACAGG TAACGTTCGA 
AACTCGGATC TACCGCTGGG TGTGCGCACG CCGGACAGTA TTATGTCCAC CGGCTCGTCG 
GGAATGTCGG GCTCCACGAA TCACATGTCC GCTTCGATGA CGCATCAGTG GCGACTACCC 
GAGAAGCTGC AGATCATCAA ACCCATGGAG GGATCGCAGA CATTGCACCA TTGGTCGCGC 
TTGGCCACGC CCACGCTCAG TGGACTGCTG GATGAGCGTC CCGGCGTAAC GATCCGTGGT 
GGACGTGGCC TGGATGATCT GGGAATGCAG ATATACACGC TGTCGGACGT AGAGGAGGAT 
GTTAGCGATG ATCTGCCCGG CAAACAGTTT GAAGCACCGG GCTGCACGTT CACCTACACC 
AATAGCATGG TCATGCATCC GGACGATGGA TTCGTTAACG ATCTGTCATT CCTCTCGCAG 
TCGCAGATGT CGTCCCGAAT GGCCTCCACG TCGACATCAA GGCAACCCAG TTGTCCTGCC 
ACGCCTCGCG CTGGACTATC GCGCAAAAAT TCCTGCTCCA CATTTTCGGT GAACCTCGGA 
CTCGCTGGAA TGCTGAATGA GAGGGGCATC AAGGCGGTGA CGCCCAGTGC CCTCAACACG 
CCCGCCGGAC CTAACTTTTC GCCCACGGTG ACGCCATGCA ACAGCCCGGA GGGATCACCT 
CCTCGTGCCC AGTCGCCCGA GCCGCTCTTT GGTCTGCTCT CGTGTGGTGC GGATTTGATC 
CGGCGCAAAA TCGTAGGCGA TCAGCATCAG CAGCAGCAAC AGAAGCAAAG GAGCAGCCTT 
AGCAAGCAGC AGCAGCAGAA GATCATGCTC TCGCACCTGG AAAGACGTGC CCTGCGATCG 
CTGAACCTAA TTGAGAAGGT GGAGAGCATC GGACTGGAGA ACATAATAAG CGCACAGAGA 
GGCCTTGGCT CAGGAATCGC GAACCGCAGC AGTTCGCCCC TGAGCAGTGG CAGCCTACAG 
AGTCTCCACA CCAGCAGCAA CAGCATTGTG GACGACATAC ACTTCGATCG GGCACAGATC 
AAGGGTGTCC TGCATCGAGG CCTGAAATCG CCAACGCCCG CAACAGCATC AACATCTGCA 
GCTGCGGCGG CTTCAGGGCC AGGTATCTCG ACGAGCAGCA GCACGAGTGC TTACAATAGC 
GATGATAGCG ACGATCAGGG CTTGGTAATG AAAATCAAAC CGTCGAAGAG CGCGACACCC 
ACAACAACAG GAGCAGCACA AAGTCAGCCA AGTTCGGCGA CGACATCCAA CGGCACGGCG 
AGTGAAACGC GACTGAAGCA GATGCAGCGC CAGAAATCGC GCAGGCAGCT AAAGAACGGA 
ATGGCCAACC AGAGGCCAGA TCTGGGCACA ATTTCTGGCG CCGGAGGAGG CGGACGAGTG 
CGCCCGGATC TGGGAAAGGT AGCCGACAGC GGCAGCAGCA GCAAGCTCAG CACCAAGCGA 
AGTGAGGCTA AGCCTGCGGA GGAGGAAGAG GCGACACCAC AGACCATCAC ACAGGCGTTT 
GTGGGTTCAG TTAGTTCCTT GCTTTTTGGC CGCAAGGGCG GTTGGCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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milt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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