Vha68-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vha68-2
Entrez Gene ID	45012
Full Name	Vacuolar H[+] ATPase 68 kDa subunit 2
Synonyms	A subunit,ATP6V1A,CG3762,Dmel\CG3762,MENE-R6,RE30552p,Vha68,Vha68.2,l(2)01510,l(2)s4214,vha67-2,vha68-2
General protein information	Preferred Names Vacuolar H[+] ATPase 68 kDa subunit 2 Names CG3762 gene product from transcript CG3762-RF CG3762-PA CG3762-PB CG3762-PC CG3762-PD CG3762-PE CG3762-PF V-ATPase 69 kDa subunit 2 Vha68-2-PA Vha68-2-PB Vha68-2-PC Vha68-2-PD Vha68-2-PE Vha68-2-PF vacuolar ATPase vacuolar H[+] ATPase 68 kDa subunit 2 vacuolar H[+] ATPase subunit 68-2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34A3-34A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259086.2	NP_001246015.1	vacuolar H[+] ATPase 68 kDa subunit 2, isoform D
NM_001273495.1	NP_001260424.1	vacuolar H[+] ATPase 68 kDa subunit 2, isoform E
NM_143747.3	NP_652004.2	vacuolar H[+] ATPase 68 kDa subunit 2, isoform A
NM_165022.2	NP_723776.1	vacuolar H[+] ATPase 68 kDa subunit 2, isoform C
NM_165021.2	NP_723775.1	vacuolar H[+] ATPase 68 kDa subunit 2, isoform B
NM_001273496.1	NP_001260425.1	vacuolar H[+] ATPase 68 kDa subunit 2, isoform F

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC776719	XP_004948457.1
Drosophila melanogaster (fruit fly)	Vha68-2	NP_723776.1
Anopheles gambiae (African malaria mosquito)	VATA_ANOGA	XP_312843.1
Eremothecium gossypii	AGOS_ADR102W	NP_984198.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vha68-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vha68-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15215	NM_001273495.1 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant E (Vha68-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15215	NM_143747.3 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant A (Vha68-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15215	NM_001259086.2 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant D (Vha68-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15215	NM_165021.2 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant B (Vha68-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15215	NM_001273496.1 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant F (Vha68-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15215	NM_165022.2 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant C (Vha68-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vha68-2 ( NM_001259086.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246015.1 Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant D (Vha68-2), mRNA.

Price & Availability↑

CloneID	OFa15215
Clone ID Related Accession (Same CDS sequence)	NM_001259086.2 , NM_001273495.1 , NM_143747.3 , NM_165022.2 , NM_165021.2 , NM_001273496.1
Accession Version	NM_001259086.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 68 kDa subunit 2, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001259086.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAACC TTAAGCGTTT CGATGATGAG GAGCGTGAGT CCAAATATGG ACGTGTCTTC 
GCTGTCTCCG GTCCTGTCGT CACCGCCGAG GCCATGTCTG GATCAGCTAT GTACGAGTTG 
GTCCGCGTCG GCTACTACGA GCTGGTGGGC GAGATCATCC GTCTGGAGGG TGACATGGCC 
ACCATCCAGG TGTACGAGGA GACCTCTGGC GTAACTGTCG GAGATCCGGT GCTGCGTACC 
GGCAAGCCTC TTTCCGTGGA GCTGGGACCC GGTATCATGG GCAGCATCTT TGACGGTATC 
CAGCGTCCCC TGAAGGACAT TAACGAGCTG ACCGAATCCA TCTACATCCC CAAGGGTGTG 
AACGTGCCCA GTTTGTCCCG CGTGGCCAGC TGGGAGTTCA ACCCCCTGAA CGTCAAGGTC 
GGCTCCCACA TCACCGGAGG TGACCTGTAC GGTCTGGTGC ATGAGAACAC TCTGGTCAAG 
CACAAGATGA TTGTGAACCC CCGCGCCAAG GGAACAGTGC GCTACATCGC CCCCTCCGGC 
AACTACAAGG TCGACGATGT CGTCCTGGAG ACCGAGTTCG ATGGAGAGAT CACCAAGCAC 
ACCATGTTGC AGGTGTGGCC AGTGCGTCAG CCACGTCCCG TGACCGAGAA GCTGCCCGCC 
AACCACCCCC TGCTCACCGG ACAGCGTGTG CTCGACTCGC TCTTCCCCTG TGTCCAGGGC 
GGTACCACCG CCATTCCCGG AGCTTTCGGT TGCGGCAAGA CTGTGATCTC GCAGGCTCTG 
TCCAAGTACT CCAACTCCGA TGTCATCATC TACGTCGGTT GCGGTGAGCG TGGTAACGAG 
ATGTCTGAGG TACTGCGTGA CTTCCCCGAG CTGTCCGTGG AGATCGACGG TGTCACCGAG 
TCCATCATGA AGCGTACCGC CCTTGTGGCC AACACCTCCA ACATGCCTGT GGCTGCTCGT 
GAGGCCTCCA TCTACACTGG TATCACCTTG TCCGAATACT TCCGTGATAT GGGTTACAAC 
GTGTCCATGA TGGCTGATTC CACCTCCCGT TGGGCTGAGG CTCTTCGTGA AATTTCTGGT 
CGTCTCGCTG AGATGCCTGC CGATTCCGGC TACCCAGCCT ACTTGGGAGC CCGTCTGGCC 
TCCTTCTACG AGCGTGCCGG TCGCGTTAAG TGCTTGGGTA ACCCCGAGCG CGAGGGATCC 
GTGTCCATTG TCGGAGCTGT GTCTCCTCCT GGTGGTGACT TCTCCGATCC CGTGACCTCC 
GCCACTCTGG GTATCGTGCA GGTGTTCTGG GGTCTCGACA AGAAGTTGGC CCAGCGCAAG 
CACTTCCCCT CGATCAACTG GCTCATCTCC TACTCGAAGT ACATGCGTGC TCTGGATGAC 
TTCTATGACA AGAACTTCCC CGAATTCGTG CCGCTGCGTA CCAAGGTCAA GGAGATCCTG 
CAGGAGGAGG AGGATCTGTC TGAGATCGTG CAACTGGTCG GCAAGGCCTC TCTGGCCGAA 
ACCGACAAGA TCACGCTGGA GGTGGCCAAG CTGCTGAAGG ACGATTTCCT GCAGCAGAAC 
TCCTACTCCT CGTACGATCG CTTCTGCCCC TTCTACAAGA CCGTGGGCAT GTTGAGGAAC 
ATCATCGACT TCTACGACAT GGCCCGTCAC TCCGTGGAGT CTACGGCTCA GTCTGAGAAC 
AAGATCACCT GGAACGTGAT TCGTGAGGCA ATGGGCAACA TTATGTACCA GCTGTCATCC 
ATGAAGTTCA AGGACCCCGT TAAGGATGGT GAGGCCAAGA TCAAGGCTGA CTTCGAGCAG 
CTGCACGAGG ACCTGCAGCA GGCCTTCAGA AATCTGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246015.1
CDS	62..1906
Translation	MSNLKRFDDEERESKYGRVFAVSGPVVTAEAMSGSAMYELVRVGYYELVGEIIRLEGDMATIQVYEETSGVTVGDPVLRTGKPLSVELGPGIMGSIFDGIQRPLKDINELTESIYIPKGVNVPSLSRVASWEFNPLNVKVGSHITGGDLYGLVHENTLVKHKMIVNPRAKGTVRYIAPSGNYKVDDVVLETEFDGEITKHTMLQVWPVRQPRPVTEKLPANHPLLTGQRVLDSLFPCVQGGTTAIPGAFGCGKTVISQALSKYSNSDVIIYVGCGERGNEMSEVLRDFPELSVEIDGVTESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYNVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSINWLISYSKYMRALDDFYDKNFPEFVPLRTKVKEILQEEEDLSEIVQLVGKASLAETDKITLEVAKLLKDDFLQQNSYSSYDRFCPFYKTVGMLRNIIDFYDMARHSVESTAQSENKITWNVIREAMGNIMYQLSSMKFKDPVKDGEAKIKADFEQLHEDLQQAFRNLED

Target ORF information:

RefSeq Version	NM_001259086.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant D (Vha68-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259086.2

ORF Insert Sequence:

ATGTCCAACC TTAAGCGTTT CGATGATGAG GAGCGTGAGT CCAAATATGG ACGTGTCTTC 
GCTGTCTCCG GTCCTGTCGT CACCGCCGAG GCCATGTCTG GATCAGCTAT GTACGAGTTG 
GTCCGCGTCG GCTACTACGA GCTGGTGGGC GAGATCATCC GTCTGGAGGG TGACATGGCC 
ACCATCCAGG TGTACGAGGA GACCTCTGGC GTAACTGTCG GAGATCCGGT GCTGCGTACC 
GGCAAGCCTC TTTCCGTGGA GCTGGGACCC GGTATCATGG GCAGCATCTT TGACGGTATC 
CAGCGTCCCC TGAAGGACAT TAACGAGCTG ACCGAATCCA TCTACATCCC CAAGGGTGTG 
AACGTGCCCA GTTTGTCCCG CGTGGCCAGC TGGGAGTTCA ACCCCCTGAA CGTCAAGGTC 
GGCTCCCACA TCACCGGAGG TGACCTGTAC GGTCTGGTGC ATGAGAACAC TCTGGTCAAG 
CACAAGATGA TTGTGAACCC CCGCGCCAAG GGAACAGTGC GCTACATCGC CCCCTCCGGC 
AACTACAAGG TCGACGATGT CGTCCTGGAG ACCGAGTTCG ATGGAGAGAT CACCAAGCAC 
ACCATGTTGC AGGTGTGGCC AGTGCGTCAG CCACGTCCCG TGACCGAGAA GCTGCCCGCC 
AACCACCCCC TGCTCACCGG ACAGCGTGTG CTCGACTCGC TCTTCCCCTG TGTCCAGGGC 
GGTACCACCG CCATTCCCGG AGCTTTCGGT TGCGGCAAGA CTGTGATCTC GCAGGCTCTG 
TCCAAGTACT CCAACTCCGA TGTCATCATC TACGTCGGTT GCGGTGAGCG TGGTAACGAG 
ATGTCTGAGG TACTGCGTGA CTTCCCCGAG CTGTCCGTGG AGATCGACGG TGTCACCGAG 
TCCATCATGA AGCGTACCGC CCTTGTGGCC AACACCTCCA ACATGCCTGT GGCTGCTCGT 
GAGGCCTCCA TCTACACTGG TATCACCTTG TCCGAATACT TCCGTGATAT GGGTTACAAC 
GTGTCCATGA TGGCTGATTC CACCTCCCGT TGGGCTGAGG CTCTTCGTGA AATTTCTGGT 
CGTCTCGCTG AGATGCCTGC CGATTCCGGC TACCCAGCCT ACTTGGGAGC CCGTCTGGCC 
TCCTTCTACG AGCGTGCCGG TCGCGTTAAG TGCTTGGGTA ACCCCGAGCG CGAGGGATCC 
GTGTCCATTG TCGGAGCTGT GTCTCCTCCT GGTGGTGACT TCTCCGATCC CGTGACCTCC 
GCCACTCTGG GTATCGTGCA GGTGTTCTGG GGTCTCGACA AGAAGTTGGC CCAGCGCAAG 
CACTTCCCCT CGATCAACTG GCTCATCTCC TACTCGAAGT ACATGCGTGC TCTGGATGAC 
TTCTATGACA AGAACTTCCC CGAATTCGTG CCGCTGCGTA CCAAGGTCAA GGAGATCCTG 
CAGGAGGAGG AGGATCTGTC TGAGATCGTG CAACTGGTCG GCAAGGCCTC TCTGGCCGAA 
ACCGACAAGA TCACGCTGGA GGTGGCCAAG CTGCTGAAGG ACGATTTCCT GCAGCAGAAC 
TCCTACTCCT CGTACGATCG CTTCTGCCCC TTCTACAAGA CCGTGGGCAT GTTGAGGAAC 
ATCATCGACT TCTACGACAT GGCCCGTCAC TCCGTGGAGT CTACGGCTCA GTCTGAGAAC 
AAGATCACCT GGAACGTGAT TCGTGAGGCA ATGGGCAACA TTATGTACCA GCTGTCATCC 
ATGAAGTTCA AGGACCCCGT TAAGGATGGT GAGGCCAAGA TCAAGGCTGA CTTCGAGCAG 
CTGCACGAGG ACCTGCAGCA GGCCTTCAGA AATCTGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha68-2 ( NM_001273495.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260424.1 Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant E (Vha68-2), mRNA.

Price & Availability↑

CloneID	OFa15215
Clone ID Related Accession (Same CDS sequence)	NM_001259086.2 , NM_001273495.1 , NM_143747.3 , NM_165022.2 , NM_165021.2 , NM_001273496.1
Accession Version	NM_001273495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 68 kDa subunit 2, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAACC TTAAGCGTTT CGATGATGAG GAGCGTGAGT CCAAATATGG ACGTGTCTTC 
GCTGTCTCCG GTCCTGTCGT CACCGCCGAG GCCATGTCTG GATCAGCTAT GTACGAGTTG 
GTCCGCGTCG GCTACTACGA GCTGGTGGGC GAGATCATCC GTCTGGAGGG TGACATGGCC 
ACCATCCAGG TGTACGAGGA GACCTCTGGC GTAACTGTCG GAGATCCGGT GCTGCGTACC 
GGCAAGCCTC TTTCCGTGGA GCTGGGACCC GGTATCATGG GCAGCATCTT TGACGGTATC 
CAGCGTCCCC TGAAGGACAT TAACGAGCTG ACCGAATCCA TCTACATCCC CAAGGGTGTG 
AACGTGCCCA GTTTGTCCCG CGTGGCCAGC TGGGAGTTCA ACCCCCTGAA CGTCAAGGTC 
GGCTCCCACA TCACCGGAGG TGACCTGTAC GGTCTGGTGC ATGAGAACAC TCTGGTCAAG 
CACAAGATGA TTGTGAACCC CCGCGCCAAG GGAACAGTGC GCTACATCGC CCCCTCCGGC 
AACTACAAGG TCGACGATGT CGTCCTGGAG ACCGAGTTCG ATGGAGAGAT CACCAAGCAC 
ACCATGTTGC AGGTGTGGCC AGTGCGTCAG CCACGTCCCG TGACCGAGAA GCTGCCCGCC 
AACCACCCCC TGCTCACCGG ACAGCGTGTG CTCGACTCGC TCTTCCCCTG TGTCCAGGGC 
GGTACCACCG CCATTCCCGG AGCTTTCGGT TGCGGCAAGA CTGTGATCTC GCAGGCTCTG 
TCCAAGTACT CCAACTCCGA TGTCATCATC TACGTCGGTT GCGGTGAGCG TGGTAACGAG 
ATGTCTGAGG TACTGCGTGA CTTCCCCGAG CTGTCCGTGG AGATCGACGG TGTCACCGAG 
TCCATCATGA AGCGTACCGC CCTTGTGGCC AACACCTCCA ACATGCCTGT GGCTGCTCGT 
GAGGCCTCCA TCTACACTGG TATCACCTTG TCCGAATACT TCCGTGATAT GGGTTACAAC 
GTGTCCATGA TGGCTGATTC CACCTCCCGT TGGGCTGAGG CTCTTCGTGA AATTTCTGGT 
CGTCTCGCTG AGATGCCTGC CGATTCCGGC TACCCAGCCT ACTTGGGAGC CCGTCTGGCC 
TCCTTCTACG AGCGTGCCGG TCGCGTTAAG TGCTTGGGTA ACCCCGAGCG CGAGGGATCC 
GTGTCCATTG TCGGAGCTGT GTCTCCTCCT GGTGGTGACT TCTCCGATCC CGTGACCTCC 
GCCACTCTGG GTATCGTGCA GGTGTTCTGG GGTCTCGACA AGAAGTTGGC CCAGCGCAAG 
CACTTCCCCT CGATCAACTG GCTCATCTCC TACTCGAAGT ACATGCGTGC TCTGGATGAC 
TTCTATGACA AGAACTTCCC CGAATTCGTG CCGCTGCGTA CCAAGGTCAA GGAGATCCTG 
CAGGAGGAGG AGGATCTGTC TGAGATCGTG CAACTGGTCG GCAAGGCCTC TCTGGCCGAA 
ACCGACAAGA TCACGCTGGA GGTGGCCAAG CTGCTGAAGG ACGATTTCCT GCAGCAGAAC 
TCCTACTCCT CGTACGATCG CTTCTGCCCC TTCTACAAGA CCGTGGGCAT GTTGAGGAAC 
ATCATCGACT TCTACGACAT GGCCCGTCAC TCCGTGGAGT CTACGGCTCA GTCTGAGAAC 
AAGATCACCT GGAACGTGAT TCGTGAGGCA ATGGGCAACA TTATGTACCA GCTGTCATCC 
ATGAAGTTCA AGGACCCCGT TAAGGATGGT GAGGCCAAGA TCAAGGCTGA CTTCGAGCAG 
CTGCACGAGG ACCTGCAGCA GGCCTTCAGA AATCTGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260424.1
CDS	92..1936
Translation	MSNLKRFDDEERESKYGRVFAVSGPVVTAEAMSGSAMYELVRVGYYELVGEIIRLEGDMATIQVYEETSGVTVGDPVLRTGKPLSVELGPGIMGSIFDGIQRPLKDINELTESIYIPKGVNVPSLSRVASWEFNPLNVKVGSHITGGDLYGLVHENTLVKHKMIVNPRAKGTVRYIAPSGNYKVDDVVLETEFDGEITKHTMLQVWPVRQPRPVTEKLPANHPLLTGQRVLDSLFPCVQGGTTAIPGAFGCGKTVISQALSKYSNSDVIIYVGCGERGNEMSEVLRDFPELSVEIDGVTESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYNVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSINWLISYSKYMRALDDFYDKNFPEFVPLRTKVKEILQEEEDLSEIVQLVGKASLAETDKITLEVAKLLKDDFLQQNSYSSYDRFCPFYKTVGMLRNIIDFYDMARHSVESTAQSENKITWNVIREAMGNIMYQLSSMKFKDPVKDGEAKIKADFEQLHEDLQQAFRNLED

Target ORF information:

RefSeq Version	NM_001273495.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant E (Vha68-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273495.1

ORF Insert Sequence:

ATGTCCAACC TTAAGCGTTT CGATGATGAG GAGCGTGAGT CCAAATATGG ACGTGTCTTC 
GCTGTCTCCG GTCCTGTCGT CACCGCCGAG GCCATGTCTG GATCAGCTAT GTACGAGTTG 
GTCCGCGTCG GCTACTACGA GCTGGTGGGC GAGATCATCC GTCTGGAGGG TGACATGGCC 
ACCATCCAGG TGTACGAGGA GACCTCTGGC GTAACTGTCG GAGATCCGGT GCTGCGTACC 
GGCAAGCCTC TTTCCGTGGA GCTGGGACCC GGTATCATGG GCAGCATCTT TGACGGTATC 
CAGCGTCCCC TGAAGGACAT TAACGAGCTG ACCGAATCCA TCTACATCCC CAAGGGTGTG 
AACGTGCCCA GTTTGTCCCG CGTGGCCAGC TGGGAGTTCA ACCCCCTGAA CGTCAAGGTC 
GGCTCCCACA TCACCGGAGG TGACCTGTAC GGTCTGGTGC ATGAGAACAC TCTGGTCAAG 
CACAAGATGA TTGTGAACCC CCGCGCCAAG GGAACAGTGC GCTACATCGC CCCCTCCGGC 
AACTACAAGG TCGACGATGT CGTCCTGGAG ACCGAGTTCG ATGGAGAGAT CACCAAGCAC 
ACCATGTTGC AGGTGTGGCC AGTGCGTCAG CCACGTCCCG TGACCGAGAA GCTGCCCGCC 
AACCACCCCC TGCTCACCGG ACAGCGTGTG CTCGACTCGC TCTTCCCCTG TGTCCAGGGC 
GGTACCACCG CCATTCCCGG AGCTTTCGGT TGCGGCAAGA CTGTGATCTC GCAGGCTCTG 
TCCAAGTACT CCAACTCCGA TGTCATCATC TACGTCGGTT GCGGTGAGCG TGGTAACGAG 
ATGTCTGAGG TACTGCGTGA CTTCCCCGAG CTGTCCGTGG AGATCGACGG TGTCACCGAG 
TCCATCATGA AGCGTACCGC CCTTGTGGCC AACACCTCCA ACATGCCTGT GGCTGCTCGT 
GAGGCCTCCA TCTACACTGG TATCACCTTG TCCGAATACT TCCGTGATAT GGGTTACAAC 
GTGTCCATGA TGGCTGATTC CACCTCCCGT TGGGCTGAGG CTCTTCGTGA AATTTCTGGT 
CGTCTCGCTG AGATGCCTGC CGATTCCGGC TACCCAGCCT ACTTGGGAGC CCGTCTGGCC 
TCCTTCTACG AGCGTGCCGG TCGCGTTAAG TGCTTGGGTA ACCCCGAGCG CGAGGGATCC 
GTGTCCATTG TCGGAGCTGT GTCTCCTCCT GGTGGTGACT TCTCCGATCC CGTGACCTCC 
GCCACTCTGG GTATCGTGCA GGTGTTCTGG GGTCTCGACA AGAAGTTGGC CCAGCGCAAG 
CACTTCCCCT CGATCAACTG GCTCATCTCC TACTCGAAGT ACATGCGTGC TCTGGATGAC 
TTCTATGACA AGAACTTCCC CGAATTCGTG CCGCTGCGTA CCAAGGTCAA GGAGATCCTG 
CAGGAGGAGG AGGATCTGTC TGAGATCGTG CAACTGGTCG GCAAGGCCTC TCTGGCCGAA 
ACCGACAAGA TCACGCTGGA GGTGGCCAAG CTGCTGAAGG ACGATTTCCT GCAGCAGAAC 
TCCTACTCCT CGTACGATCG CTTCTGCCCC TTCTACAAGA CCGTGGGCAT GTTGAGGAAC 
ATCATCGACT TCTACGACAT GGCCCGTCAC TCCGTGGAGT CTACGGCTCA GTCTGAGAAC 
AAGATCACCT GGAACGTGAT TCGTGAGGCA ATGGGCAACA TTATGTACCA GCTGTCATCC 
ATGAAGTTCA AGGACCCCGT TAAGGATGGT GAGGCCAAGA TCAAGGCTGA CTTCGAGCAG 
CTGCACGAGG ACCTGCAGCA GGCCTTCAGA AATCTGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha68-2 ( NM_143747.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652004.2 Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant A (Vha68-2), mRNA.

Price & Availability↑

CloneID	OFa15215
Clone ID Related Accession (Same CDS sequence)	NM_001259086.2 , NM_001273495.1 , NM_143747.3 , NM_165022.2 , NM_165021.2 , NM_001273496.1
Accession Version	NM_143747.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 68 kDa subunit 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_143747.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAACC TTAAGCGTTT CGATGATGAG GAGCGTGAGT CCAAATATGG ACGTGTCTTC 
GCTGTCTCCG GTCCTGTCGT CACCGCCGAG GCCATGTCTG GATCAGCTAT GTACGAGTTG 
GTCCGCGTCG GCTACTACGA GCTGGTGGGC GAGATCATCC GTCTGGAGGG TGACATGGCC 
ACCATCCAGG TGTACGAGGA GACCTCTGGC GTAACTGTCG GAGATCCGGT GCTGCGTACC 
GGCAAGCCTC TTTCCGTGGA GCTGGGACCC GGTATCATGG GCAGCATCTT TGACGGTATC 
CAGCGTCCCC TGAAGGACAT TAACGAGCTG ACCGAATCCA TCTACATCCC CAAGGGTGTG 
AACGTGCCCA GTTTGTCCCG CGTGGCCAGC TGGGAGTTCA ACCCCCTGAA CGTCAAGGTC 
GGCTCCCACA TCACCGGAGG TGACCTGTAC GGTCTGGTGC ATGAGAACAC TCTGGTCAAG 
CACAAGATGA TTGTGAACCC CCGCGCCAAG GGAACAGTGC GCTACATCGC CCCCTCCGGC 
AACTACAAGG TCGACGATGT CGTCCTGGAG ACCGAGTTCG ATGGAGAGAT CACCAAGCAC 
ACCATGTTGC AGGTGTGGCC AGTGCGTCAG CCACGTCCCG TGACCGAGAA GCTGCCCGCC 
AACCACCCCC TGCTCACCGG ACAGCGTGTG CTCGACTCGC TCTTCCCCTG TGTCCAGGGC 
GGTACCACCG CCATTCCCGG AGCTTTCGGT TGCGGCAAGA CTGTGATCTC GCAGGCTCTG 
TCCAAGTACT CCAACTCCGA TGTCATCATC TACGTCGGTT GCGGTGAGCG TGGTAACGAG 
ATGTCTGAGG TACTGCGTGA CTTCCCCGAG CTGTCCGTGG AGATCGACGG TGTCACCGAG 
TCCATCATGA AGCGTACCGC CCTTGTGGCC AACACCTCCA ACATGCCTGT GGCTGCTCGT 
GAGGCCTCCA TCTACACTGG TATCACCTTG TCCGAATACT TCCGTGATAT GGGTTACAAC 
GTGTCCATGA TGGCTGATTC CACCTCCCGT TGGGCTGAGG CTCTTCGTGA AATTTCTGGT 
CGTCTCGCTG AGATGCCTGC CGATTCCGGC TACCCAGCCT ACTTGGGAGC CCGTCTGGCC 
TCCTTCTACG AGCGTGCCGG TCGCGTTAAG TGCTTGGGTA ACCCCGAGCG CGAGGGATCC 
GTGTCCATTG TCGGAGCTGT GTCTCCTCCT GGTGGTGACT TCTCCGATCC CGTGACCTCC 
GCCACTCTGG GTATCGTGCA GGTGTTCTGG GGTCTCGACA AGAAGTTGGC CCAGCGCAAG 
CACTTCCCCT CGATCAACTG GCTCATCTCC TACTCGAAGT ACATGCGTGC TCTGGATGAC 
TTCTATGACA AGAACTTCCC CGAATTCGTG CCGCTGCGTA CCAAGGTCAA GGAGATCCTG 
CAGGAGGAGG AGGATCTGTC TGAGATCGTG CAACTGGTCG GCAAGGCCTC TCTGGCCGAA 
ACCGACAAGA TCACGCTGGA GGTGGCCAAG CTGCTGAAGG ACGATTTCCT GCAGCAGAAC 
TCCTACTCCT CGTACGATCG CTTCTGCCCC TTCTACAAGA CCGTGGGCAT GTTGAGGAAC 
ATCATCGACT TCTACGACAT GGCCCGTCAC TCCGTGGAGT CTACGGCTCA GTCTGAGAAC 
AAGATCACCT GGAACGTGAT TCGTGAGGCA ATGGGCAACA TTATGTACCA GCTGTCATCC 
ATGAAGTTCA AGGACCCCGT TAAGGATGGT GAGGCCAAGA TCAAGGCTGA CTTCGAGCAG 
CTGCACGAGG ACCTGCAGCA GGCCTTCAGA AATCTGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652004.2
CDS	104..1948
Translation	MSNLKRFDDEERESKYGRVFAVSGPVVTAEAMSGSAMYELVRVGYYELVGEIIRLEGDMATIQVYEETSGVTVGDPVLRTGKPLSVELGPGIMGSIFDGIQRPLKDINELTESIYIPKGVNVPSLSRVASWEFNPLNVKVGSHITGGDLYGLVHENTLVKHKMIVNPRAKGTVRYIAPSGNYKVDDVVLETEFDGEITKHTMLQVWPVRQPRPVTEKLPANHPLLTGQRVLDSLFPCVQGGTTAIPGAFGCGKTVISQALSKYSNSDVIIYVGCGERGNEMSEVLRDFPELSVEIDGVTESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYNVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSINWLISYSKYMRALDDFYDKNFPEFVPLRTKVKEILQEEEDLSEIVQLVGKASLAETDKITLEVAKLLKDDFLQQNSYSSYDRFCPFYKTVGMLRNIIDFYDMARHSVESTAQSENKITWNVIREAMGNIMYQLSSMKFKDPVKDGEAKIKADFEQLHEDLQQAFRNLED

Target ORF information:

RefSeq Version	NM_143747.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant A (Vha68-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143747.3

ORF Insert Sequence:

ATGTCCAACC TTAAGCGTTT CGATGATGAG GAGCGTGAGT CCAAATATGG ACGTGTCTTC 
GCTGTCTCCG GTCCTGTCGT CACCGCCGAG GCCATGTCTG GATCAGCTAT GTACGAGTTG 
GTCCGCGTCG GCTACTACGA GCTGGTGGGC GAGATCATCC GTCTGGAGGG TGACATGGCC 
ACCATCCAGG TGTACGAGGA GACCTCTGGC GTAACTGTCG GAGATCCGGT GCTGCGTACC 
GGCAAGCCTC TTTCCGTGGA GCTGGGACCC GGTATCATGG GCAGCATCTT TGACGGTATC 
CAGCGTCCCC TGAAGGACAT TAACGAGCTG ACCGAATCCA TCTACATCCC CAAGGGTGTG 
AACGTGCCCA GTTTGTCCCG CGTGGCCAGC TGGGAGTTCA ACCCCCTGAA CGTCAAGGTC 
GGCTCCCACA TCACCGGAGG TGACCTGTAC GGTCTGGTGC ATGAGAACAC TCTGGTCAAG 
CACAAGATGA TTGTGAACCC CCGCGCCAAG GGAACAGTGC GCTACATCGC CCCCTCCGGC 
AACTACAAGG TCGACGATGT CGTCCTGGAG ACCGAGTTCG ATGGAGAGAT CACCAAGCAC 
ACCATGTTGC AGGTGTGGCC AGTGCGTCAG CCACGTCCCG TGACCGAGAA GCTGCCCGCC 
AACCACCCCC TGCTCACCGG ACAGCGTGTG CTCGACTCGC TCTTCCCCTG TGTCCAGGGC 
GGTACCACCG CCATTCCCGG AGCTTTCGGT TGCGGCAAGA CTGTGATCTC GCAGGCTCTG 
TCCAAGTACT CCAACTCCGA TGTCATCATC TACGTCGGTT GCGGTGAGCG TGGTAACGAG 
ATGTCTGAGG TACTGCGTGA CTTCCCCGAG CTGTCCGTGG AGATCGACGG TGTCACCGAG 
TCCATCATGA AGCGTACCGC CCTTGTGGCC AACACCTCCA ACATGCCTGT GGCTGCTCGT 
GAGGCCTCCA TCTACACTGG TATCACCTTG TCCGAATACT TCCGTGATAT GGGTTACAAC 
GTGTCCATGA TGGCTGATTC CACCTCCCGT TGGGCTGAGG CTCTTCGTGA AATTTCTGGT 
CGTCTCGCTG AGATGCCTGC CGATTCCGGC TACCCAGCCT ACTTGGGAGC CCGTCTGGCC 
TCCTTCTACG AGCGTGCCGG TCGCGTTAAG TGCTTGGGTA ACCCCGAGCG CGAGGGATCC 
GTGTCCATTG TCGGAGCTGT GTCTCCTCCT GGTGGTGACT TCTCCGATCC CGTGACCTCC 
GCCACTCTGG GTATCGTGCA GGTGTTCTGG GGTCTCGACA AGAAGTTGGC CCAGCGCAAG 
CACTTCCCCT CGATCAACTG GCTCATCTCC TACTCGAAGT ACATGCGTGC TCTGGATGAC 
TTCTATGACA AGAACTTCCC CGAATTCGTG CCGCTGCGTA CCAAGGTCAA GGAGATCCTG 
CAGGAGGAGG AGGATCTGTC TGAGATCGTG CAACTGGTCG GCAAGGCCTC TCTGGCCGAA 
ACCGACAAGA TCACGCTGGA GGTGGCCAAG CTGCTGAAGG ACGATTTCCT GCAGCAGAAC 
TCCTACTCCT CGTACGATCG CTTCTGCCCC TTCTACAAGA CCGTGGGCAT GTTGAGGAAC 
ATCATCGACT TCTACGACAT GGCCCGTCAC TCCGTGGAGT CTACGGCTCA GTCTGAGAAC 
AAGATCACCT GGAACGTGAT TCGTGAGGCA ATGGGCAACA TTATGTACCA GCTGTCATCC 
ATGAAGTTCA AGGACCCCGT TAAGGATGGT GAGGCCAAGA TCAAGGCTGA CTTCGAGCAG 
CTGCACGAGG ACCTGCAGCA GGCCTTCAGA AATCTGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha68-2 ( NM_165022.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723776.1 Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant C (Vha68-2), mRNA.

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CloneID	OFa15215
Clone ID Related Accession (Same CDS sequence)	NM_001259086.2 , NM_001273495.1 , NM_143747.3 , NM_165022.2 , NM_165021.2 , NM_001273496.1
Accession Version	NM_165022.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 68 kDa subunit 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165022.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAACC TTAAGCGTTT CGATGATGAG GAGCGTGAGT CCAAATATGG ACGTGTCTTC 
GCTGTCTCCG GTCCTGTCGT CACCGCCGAG GCCATGTCTG GATCAGCTAT GTACGAGTTG 
GTCCGCGTCG GCTACTACGA GCTGGTGGGC GAGATCATCC GTCTGGAGGG TGACATGGCC 
ACCATCCAGG TGTACGAGGA GACCTCTGGC GTAACTGTCG GAGATCCGGT GCTGCGTACC 
GGCAAGCCTC TTTCCGTGGA GCTGGGACCC GGTATCATGG GCAGCATCTT TGACGGTATC 
CAGCGTCCCC TGAAGGACAT TAACGAGCTG ACCGAATCCA TCTACATCCC CAAGGGTGTG 
AACGTGCCCA GTTTGTCCCG CGTGGCCAGC TGGGAGTTCA ACCCCCTGAA CGTCAAGGTC 
GGCTCCCACA TCACCGGAGG TGACCTGTAC GGTCTGGTGC ATGAGAACAC TCTGGTCAAG 
CACAAGATGA TTGTGAACCC CCGCGCCAAG GGAACAGTGC GCTACATCGC CCCCTCCGGC 
AACTACAAGG TCGACGATGT CGTCCTGGAG ACCGAGTTCG ATGGAGAGAT CACCAAGCAC 
ACCATGTTGC AGGTGTGGCC AGTGCGTCAG CCACGTCCCG TGACCGAGAA GCTGCCCGCC 
AACCACCCCC TGCTCACCGG ACAGCGTGTG CTCGACTCGC TCTTCCCCTG TGTCCAGGGC 
GGTACCACCG CCATTCCCGG AGCTTTCGGT TGCGGCAAGA CTGTGATCTC GCAGGCTCTG 
TCCAAGTACT CCAACTCCGA TGTCATCATC TACGTCGGTT GCGGTGAGCG TGGTAACGAG 
ATGTCTGAGG TACTGCGTGA CTTCCCCGAG CTGTCCGTGG AGATCGACGG TGTCACCGAG 
TCCATCATGA AGCGTACCGC CCTTGTGGCC AACACCTCCA ACATGCCTGT GGCTGCTCGT 
GAGGCCTCCA TCTACACTGG TATCACCTTG TCCGAATACT TCCGTGATAT GGGTTACAAC 
GTGTCCATGA TGGCTGATTC CACCTCCCGT TGGGCTGAGG CTCTTCGTGA AATTTCTGGT 
CGTCTCGCTG AGATGCCTGC CGATTCCGGC TACCCAGCCT ACTTGGGAGC CCGTCTGGCC 
TCCTTCTACG AGCGTGCCGG TCGCGTTAAG TGCTTGGGTA ACCCCGAGCG CGAGGGATCC 
GTGTCCATTG TCGGAGCTGT GTCTCCTCCT GGTGGTGACT TCTCCGATCC CGTGACCTCC 
GCCACTCTGG GTATCGTGCA GGTGTTCTGG GGTCTCGACA AGAAGTTGGC CCAGCGCAAG 
CACTTCCCCT CGATCAACTG GCTCATCTCC TACTCGAAGT ACATGCGTGC TCTGGATGAC 
TTCTATGACA AGAACTTCCC CGAATTCGTG CCGCTGCGTA CCAAGGTCAA GGAGATCCTG 
CAGGAGGAGG AGGATCTGTC TGAGATCGTG CAACTGGTCG GCAAGGCCTC TCTGGCCGAA 
ACCGACAAGA TCACGCTGGA GGTGGCCAAG CTGCTGAAGG ACGATTTCCT GCAGCAGAAC 
TCCTACTCCT CGTACGATCG CTTCTGCCCC TTCTACAAGA CCGTGGGCAT GTTGAGGAAC 
ATCATCGACT TCTACGACAT GGCCCGTCAC TCCGTGGAGT CTACGGCTCA GTCTGAGAAC 
AAGATCACCT GGAACGTGAT TCGTGAGGCA ATGGGCAACA TTATGTACCA GCTGTCATCC 
ATGAAGTTCA AGGACCCCGT TAAGGATGGT GAGGCCAAGA TCAAGGCTGA CTTCGAGCAG 
CTGCACGAGG ACCTGCAGCA GGCCTTCAGA AATCTGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723776.1
CDS	149..1993
Translation	MSNLKRFDDEERESKYGRVFAVSGPVVTAEAMSGSAMYELVRVGYYELVGEIIRLEGDMATIQVYEETSGVTVGDPVLRTGKPLSVELGPGIMGSIFDGIQRPLKDINELTESIYIPKGVNVPSLSRVASWEFNPLNVKVGSHITGGDLYGLVHENTLVKHKMIVNPRAKGTVRYIAPSGNYKVDDVVLETEFDGEITKHTMLQVWPVRQPRPVTEKLPANHPLLTGQRVLDSLFPCVQGGTTAIPGAFGCGKTVISQALSKYSNSDVIIYVGCGERGNEMSEVLRDFPELSVEIDGVTESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYNVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSINWLISYSKYMRALDDFYDKNFPEFVPLRTKVKEILQEEEDLSEIVQLVGKASLAETDKITLEVAKLLKDDFLQQNSYSSYDRFCPFYKTVGMLRNIIDFYDMARHSVESTAQSENKITWNVIREAMGNIMYQLSSMKFKDPVKDGEAKIKADFEQLHEDLQQAFRNLED

Target ORF information:

RefSeq Version	NM_165022.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant C (Vha68-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165022.2

ORF Insert Sequence:

ATGTCCAACC TTAAGCGTTT CGATGATGAG GAGCGTGAGT CCAAATATGG ACGTGTCTTC 
GCTGTCTCCG GTCCTGTCGT CACCGCCGAG GCCATGTCTG GATCAGCTAT GTACGAGTTG 
GTCCGCGTCG GCTACTACGA GCTGGTGGGC GAGATCATCC GTCTGGAGGG TGACATGGCC 
ACCATCCAGG TGTACGAGGA GACCTCTGGC GTAACTGTCG GAGATCCGGT GCTGCGTACC 
GGCAAGCCTC TTTCCGTGGA GCTGGGACCC GGTATCATGG GCAGCATCTT TGACGGTATC 
CAGCGTCCCC TGAAGGACAT TAACGAGCTG ACCGAATCCA TCTACATCCC CAAGGGTGTG 
AACGTGCCCA GTTTGTCCCG CGTGGCCAGC TGGGAGTTCA ACCCCCTGAA CGTCAAGGTC 
GGCTCCCACA TCACCGGAGG TGACCTGTAC GGTCTGGTGC ATGAGAACAC TCTGGTCAAG 
CACAAGATGA TTGTGAACCC CCGCGCCAAG GGAACAGTGC GCTACATCGC CCCCTCCGGC 
AACTACAAGG TCGACGATGT CGTCCTGGAG ACCGAGTTCG ATGGAGAGAT CACCAAGCAC 
ACCATGTTGC AGGTGTGGCC AGTGCGTCAG CCACGTCCCG TGACCGAGAA GCTGCCCGCC 
AACCACCCCC TGCTCACCGG ACAGCGTGTG CTCGACTCGC TCTTCCCCTG TGTCCAGGGC 
GGTACCACCG CCATTCCCGG AGCTTTCGGT TGCGGCAAGA CTGTGATCTC GCAGGCTCTG 
TCCAAGTACT CCAACTCCGA TGTCATCATC TACGTCGGTT GCGGTGAGCG TGGTAACGAG 
ATGTCTGAGG TACTGCGTGA CTTCCCCGAG CTGTCCGTGG AGATCGACGG TGTCACCGAG 
TCCATCATGA AGCGTACCGC CCTTGTGGCC AACACCTCCA ACATGCCTGT GGCTGCTCGT 
GAGGCCTCCA TCTACACTGG TATCACCTTG TCCGAATACT TCCGTGATAT GGGTTACAAC 
GTGTCCATGA TGGCTGATTC CACCTCCCGT TGGGCTGAGG CTCTTCGTGA AATTTCTGGT 
CGTCTCGCTG AGATGCCTGC CGATTCCGGC TACCCAGCCT ACTTGGGAGC CCGTCTGGCC 
TCCTTCTACG AGCGTGCCGG TCGCGTTAAG TGCTTGGGTA ACCCCGAGCG CGAGGGATCC 
GTGTCCATTG TCGGAGCTGT GTCTCCTCCT GGTGGTGACT TCTCCGATCC CGTGACCTCC 
GCCACTCTGG GTATCGTGCA GGTGTTCTGG GGTCTCGACA AGAAGTTGGC CCAGCGCAAG 
CACTTCCCCT CGATCAACTG GCTCATCTCC TACTCGAAGT ACATGCGTGC TCTGGATGAC 
TTCTATGACA AGAACTTCCC CGAATTCGTG CCGCTGCGTA CCAAGGTCAA GGAGATCCTG 
CAGGAGGAGG AGGATCTGTC TGAGATCGTG CAACTGGTCG GCAAGGCCTC TCTGGCCGAA 
ACCGACAAGA TCACGCTGGA GGTGGCCAAG CTGCTGAAGG ACGATTTCCT GCAGCAGAAC 
TCCTACTCCT CGTACGATCG CTTCTGCCCC TTCTACAAGA CCGTGGGCAT GTTGAGGAAC 
ATCATCGACT TCTACGACAT GGCCCGTCAC TCCGTGGAGT CTACGGCTCA GTCTGAGAAC 
AAGATCACCT GGAACGTGAT TCGTGAGGCA ATGGGCAACA TTATGTACCA GCTGTCATCC 
ATGAAGTTCA AGGACCCCGT TAAGGATGGT GAGGCCAAGA TCAAGGCTGA CTTCGAGCAG 
CTGCACGAGG ACCTGCAGCA GGCCTTCAGA AATCTGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha68-2 ( NM_165021.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723775.1 Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant B (Vha68-2), mRNA.

Price & Availability↑

CloneID	OFa15215
Clone ID Related Accession (Same CDS sequence)	NM_001259086.2 , NM_001273495.1 , NM_143747.3 , NM_165022.2 , NM_165021.2 , NM_001273496.1
Accession Version	NM_165021.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 68 kDa subunit 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165021.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAACC TTAAGCGTTT CGATGATGAG GAGCGTGAGT CCAAATATGG ACGTGTCTTC 
GCTGTCTCCG GTCCTGTCGT CACCGCCGAG GCCATGTCTG GATCAGCTAT GTACGAGTTG 
GTCCGCGTCG GCTACTACGA GCTGGTGGGC GAGATCATCC GTCTGGAGGG TGACATGGCC 
ACCATCCAGG TGTACGAGGA GACCTCTGGC GTAACTGTCG GAGATCCGGT GCTGCGTACC 
GGCAAGCCTC TTTCCGTGGA GCTGGGACCC GGTATCATGG GCAGCATCTT TGACGGTATC 
CAGCGTCCCC TGAAGGACAT TAACGAGCTG ACCGAATCCA TCTACATCCC CAAGGGTGTG 
AACGTGCCCA GTTTGTCCCG CGTGGCCAGC TGGGAGTTCA ACCCCCTGAA CGTCAAGGTC 
GGCTCCCACA TCACCGGAGG TGACCTGTAC GGTCTGGTGC ATGAGAACAC TCTGGTCAAG 
CACAAGATGA TTGTGAACCC CCGCGCCAAG GGAACAGTGC GCTACATCGC CCCCTCCGGC 
AACTACAAGG TCGACGATGT CGTCCTGGAG ACCGAGTTCG ATGGAGAGAT CACCAAGCAC 
ACCATGTTGC AGGTGTGGCC AGTGCGTCAG CCACGTCCCG TGACCGAGAA GCTGCCCGCC 
AACCACCCCC TGCTCACCGG ACAGCGTGTG CTCGACTCGC TCTTCCCCTG TGTCCAGGGC 
GGTACCACCG CCATTCCCGG AGCTTTCGGT TGCGGCAAGA CTGTGATCTC GCAGGCTCTG 
TCCAAGTACT CCAACTCCGA TGTCATCATC TACGTCGGTT GCGGTGAGCG TGGTAACGAG 
ATGTCTGAGG TACTGCGTGA CTTCCCCGAG CTGTCCGTGG AGATCGACGG TGTCACCGAG 
TCCATCATGA AGCGTACCGC CCTTGTGGCC AACACCTCCA ACATGCCTGT GGCTGCTCGT 
GAGGCCTCCA TCTACACTGG TATCACCTTG TCCGAATACT TCCGTGATAT GGGTTACAAC 
GTGTCCATGA TGGCTGATTC CACCTCCCGT TGGGCTGAGG CTCTTCGTGA AATTTCTGGT 
CGTCTCGCTG AGATGCCTGC CGATTCCGGC TACCCAGCCT ACTTGGGAGC CCGTCTGGCC 
TCCTTCTACG AGCGTGCCGG TCGCGTTAAG TGCTTGGGTA ACCCCGAGCG CGAGGGATCC 
GTGTCCATTG TCGGAGCTGT GTCTCCTCCT GGTGGTGACT TCTCCGATCC CGTGACCTCC 
GCCACTCTGG GTATCGTGCA GGTGTTCTGG GGTCTCGACA AGAAGTTGGC CCAGCGCAAG 
CACTTCCCCT CGATCAACTG GCTCATCTCC TACTCGAAGT ACATGCGTGC TCTGGATGAC 
TTCTATGACA AGAACTTCCC CGAATTCGTG CCGCTGCGTA CCAAGGTCAA GGAGATCCTG 
CAGGAGGAGG AGGATCTGTC TGAGATCGTG CAACTGGTCG GCAAGGCCTC TCTGGCCGAA 
ACCGACAAGA TCACGCTGGA GGTGGCCAAG CTGCTGAAGG ACGATTTCCT GCAGCAGAAC 
TCCTACTCCT CGTACGATCG CTTCTGCCCC TTCTACAAGA CCGTGGGCAT GTTGAGGAAC 
ATCATCGACT TCTACGACAT GGCCCGTCAC TCCGTGGAGT CTACGGCTCA GTCTGAGAAC 
AAGATCACCT GGAACGTGAT TCGTGAGGCA ATGGGCAACA TTATGTACCA GCTGTCATCC 
ATGAAGTTCA AGGACCCCGT TAAGGATGGT GAGGCCAAGA TCAAGGCTGA CTTCGAGCAG 
CTGCACGAGG ACCTGCAGCA GGCCTTCAGA AATCTGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723775.1
CDS	82..1926
Translation	MSNLKRFDDEERESKYGRVFAVSGPVVTAEAMSGSAMYELVRVGYYELVGEIIRLEGDMATIQVYEETSGVTVGDPVLRTGKPLSVELGPGIMGSIFDGIQRPLKDINELTESIYIPKGVNVPSLSRVASWEFNPLNVKVGSHITGGDLYGLVHENTLVKHKMIVNPRAKGTVRYIAPSGNYKVDDVVLETEFDGEITKHTMLQVWPVRQPRPVTEKLPANHPLLTGQRVLDSLFPCVQGGTTAIPGAFGCGKTVISQALSKYSNSDVIIYVGCGERGNEMSEVLRDFPELSVEIDGVTESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYNVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSINWLISYSKYMRALDDFYDKNFPEFVPLRTKVKEILQEEEDLSEIVQLVGKASLAETDKITLEVAKLLKDDFLQQNSYSSYDRFCPFYKTVGMLRNIIDFYDMARHSVESTAQSENKITWNVIREAMGNIMYQLSSMKFKDPVKDGEAKIKADFEQLHEDLQQAFRNLED

Target ORF information:

RefSeq Version	NM_165021.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant B (Vha68-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165021.2

ORF Insert Sequence:

ATGTCCAACC TTAAGCGTTT CGATGATGAG GAGCGTGAGT CCAAATATGG ACGTGTCTTC 
GCTGTCTCCG GTCCTGTCGT CACCGCCGAG GCCATGTCTG GATCAGCTAT GTACGAGTTG 
GTCCGCGTCG GCTACTACGA GCTGGTGGGC GAGATCATCC GTCTGGAGGG TGACATGGCC 
ACCATCCAGG TGTACGAGGA GACCTCTGGC GTAACTGTCG GAGATCCGGT GCTGCGTACC 
GGCAAGCCTC TTTCCGTGGA GCTGGGACCC GGTATCATGG GCAGCATCTT TGACGGTATC 
CAGCGTCCCC TGAAGGACAT TAACGAGCTG ACCGAATCCA TCTACATCCC CAAGGGTGTG 
AACGTGCCCA GTTTGTCCCG CGTGGCCAGC TGGGAGTTCA ACCCCCTGAA CGTCAAGGTC 
GGCTCCCACA TCACCGGAGG TGACCTGTAC GGTCTGGTGC ATGAGAACAC TCTGGTCAAG 
CACAAGATGA TTGTGAACCC CCGCGCCAAG GGAACAGTGC GCTACATCGC CCCCTCCGGC 
AACTACAAGG TCGACGATGT CGTCCTGGAG ACCGAGTTCG ATGGAGAGAT CACCAAGCAC 
ACCATGTTGC AGGTGTGGCC AGTGCGTCAG CCACGTCCCG TGACCGAGAA GCTGCCCGCC 
AACCACCCCC TGCTCACCGG ACAGCGTGTG CTCGACTCGC TCTTCCCCTG TGTCCAGGGC 
GGTACCACCG CCATTCCCGG AGCTTTCGGT TGCGGCAAGA CTGTGATCTC GCAGGCTCTG 
TCCAAGTACT CCAACTCCGA TGTCATCATC TACGTCGGTT GCGGTGAGCG TGGTAACGAG 
ATGTCTGAGG TACTGCGTGA CTTCCCCGAG CTGTCCGTGG AGATCGACGG TGTCACCGAG 
TCCATCATGA AGCGTACCGC CCTTGTGGCC AACACCTCCA ACATGCCTGT GGCTGCTCGT 
GAGGCCTCCA TCTACACTGG TATCACCTTG TCCGAATACT TCCGTGATAT GGGTTACAAC 
GTGTCCATGA TGGCTGATTC CACCTCCCGT TGGGCTGAGG CTCTTCGTGA AATTTCTGGT 
CGTCTCGCTG AGATGCCTGC CGATTCCGGC TACCCAGCCT ACTTGGGAGC CCGTCTGGCC 
TCCTTCTACG AGCGTGCCGG TCGCGTTAAG TGCTTGGGTA ACCCCGAGCG CGAGGGATCC 
GTGTCCATTG TCGGAGCTGT GTCTCCTCCT GGTGGTGACT TCTCCGATCC CGTGACCTCC 
GCCACTCTGG GTATCGTGCA GGTGTTCTGG GGTCTCGACA AGAAGTTGGC CCAGCGCAAG 
CACTTCCCCT CGATCAACTG GCTCATCTCC TACTCGAAGT ACATGCGTGC TCTGGATGAC 
TTCTATGACA AGAACTTCCC CGAATTCGTG CCGCTGCGTA CCAAGGTCAA GGAGATCCTG 
CAGGAGGAGG AGGATCTGTC TGAGATCGTG CAACTGGTCG GCAAGGCCTC TCTGGCCGAA 
ACCGACAAGA TCACGCTGGA GGTGGCCAAG CTGCTGAAGG ACGATTTCCT GCAGCAGAAC 
TCCTACTCCT CGTACGATCG CTTCTGCCCC TTCTACAAGA CCGTGGGCAT GTTGAGGAAC 
ATCATCGACT TCTACGACAT GGCCCGTCAC TCCGTGGAGT CTACGGCTCA GTCTGAGAAC 
AAGATCACCT GGAACGTGAT TCGTGAGGCA ATGGGCAACA TTATGTACCA GCTGTCATCC 
ATGAAGTTCA AGGACCCCGT TAAGGATGGT GAGGCCAAGA TCAAGGCTGA CTTCGAGCAG 
CTGCACGAGG ACCTGCAGCA GGCCTTCAGA AATCTGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha68-2 ( NM_001273496.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260425.1 Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant F (Vha68-2), mRNA.

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CloneID	OFa15215
Clone ID Related Accession (Same CDS sequence)	NM_001259086.2 , NM_001273495.1 , NM_143747.3 , NM_165022.2 , NM_165021.2 , NM_001273496.1
Accession Version	NM_001273496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 68 kDa subunit 2, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAACC TTAAGCGTTT CGATGATGAG GAGCGTGAGT CCAAATATGG ACGTGTCTTC 
GCTGTCTCCG GTCCTGTCGT CACCGCCGAG GCCATGTCTG GATCAGCTAT GTACGAGTTG 
GTCCGCGTCG GCTACTACGA GCTGGTGGGC GAGATCATCC GTCTGGAGGG TGACATGGCC 
ACCATCCAGG TGTACGAGGA GACCTCTGGC GTAACTGTCG GAGATCCGGT GCTGCGTACC 
GGCAAGCCTC TTTCCGTGGA GCTGGGACCC GGTATCATGG GCAGCATCTT TGACGGTATC 
CAGCGTCCCC TGAAGGACAT TAACGAGCTG ACCGAATCCA TCTACATCCC CAAGGGTGTG 
AACGTGCCCA GTTTGTCCCG CGTGGCCAGC TGGGAGTTCA ACCCCCTGAA CGTCAAGGTC 
GGCTCCCACA TCACCGGAGG TGACCTGTAC GGTCTGGTGC ATGAGAACAC TCTGGTCAAG 
CACAAGATGA TTGTGAACCC CCGCGCCAAG GGAACAGTGC GCTACATCGC CCCCTCCGGC 
AACTACAAGG TCGACGATGT CGTCCTGGAG ACCGAGTTCG ATGGAGAGAT CACCAAGCAC 
ACCATGTTGC AGGTGTGGCC AGTGCGTCAG CCACGTCCCG TGACCGAGAA GCTGCCCGCC 
AACCACCCCC TGCTCACCGG ACAGCGTGTG CTCGACTCGC TCTTCCCCTG TGTCCAGGGC 
GGTACCACCG CCATTCCCGG AGCTTTCGGT TGCGGCAAGA CTGTGATCTC GCAGGCTCTG 
TCCAAGTACT CCAACTCCGA TGTCATCATC TACGTCGGTT GCGGTGAGCG TGGTAACGAG 
ATGTCTGAGG TACTGCGTGA CTTCCCCGAG CTGTCCGTGG AGATCGACGG TGTCACCGAG 
TCCATCATGA AGCGTACCGC CCTTGTGGCC AACACCTCCA ACATGCCTGT GGCTGCTCGT 
GAGGCCTCCA TCTACACTGG TATCACCTTG TCCGAATACT TCCGTGATAT GGGTTACAAC 
GTGTCCATGA TGGCTGATTC CACCTCCCGT TGGGCTGAGG CTCTTCGTGA AATTTCTGGT 
CGTCTCGCTG AGATGCCTGC CGATTCCGGC TACCCAGCCT ACTTGGGAGC CCGTCTGGCC 
TCCTTCTACG AGCGTGCCGG TCGCGTTAAG TGCTTGGGTA ACCCCGAGCG CGAGGGATCC 
GTGTCCATTG TCGGAGCTGT GTCTCCTCCT GGTGGTGACT TCTCCGATCC CGTGACCTCC 
GCCACTCTGG GTATCGTGCA GGTGTTCTGG GGTCTCGACA AGAAGTTGGC CCAGCGCAAG 
CACTTCCCCT CGATCAACTG GCTCATCTCC TACTCGAAGT ACATGCGTGC TCTGGATGAC 
TTCTATGACA AGAACTTCCC CGAATTCGTG CCGCTGCGTA CCAAGGTCAA GGAGATCCTG 
CAGGAGGAGG AGGATCTGTC TGAGATCGTG CAACTGGTCG GCAAGGCCTC TCTGGCCGAA 
ACCGACAAGA TCACGCTGGA GGTGGCCAAG CTGCTGAAGG ACGATTTCCT GCAGCAGAAC 
TCCTACTCCT CGTACGATCG CTTCTGCCCC TTCTACAAGA CCGTGGGCAT GTTGAGGAAC 
ATCATCGACT TCTACGACAT GGCCCGTCAC TCCGTGGAGT CTACGGCTCA GTCTGAGAAC 
AAGATCACCT GGAACGTGAT TCGTGAGGCA ATGGGCAACA TTATGTACCA GCTGTCATCC 
ATGAAGTTCA AGGACCCCGT TAAGGATGGT GAGGCCAAGA TCAAGGCTGA CTTCGAGCAG 
CTGCACGAGG ACCTGCAGCA GGCCTTCAGA AATCTGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260425.1
CDS	146..1990
Translation	MSNLKRFDDEERESKYGRVFAVSGPVVTAEAMSGSAMYELVRVGYYELVGEIIRLEGDMATIQVYEETSGVTVGDPVLRTGKPLSVELGPGIMGSIFDGIQRPLKDINELTESIYIPKGVNVPSLSRVASWEFNPLNVKVGSHITGGDLYGLVHENTLVKHKMIVNPRAKGTVRYIAPSGNYKVDDVVLETEFDGEITKHTMLQVWPVRQPRPVTEKLPANHPLLTGQRVLDSLFPCVQGGTTAIPGAFGCGKTVISQALSKYSNSDVIIYVGCGERGNEMSEVLRDFPELSVEIDGVTESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYNVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSINWLISYSKYMRALDDFYDKNFPEFVPLRTKVKEILQEEEDLSEIVQLVGKASLAETDKITLEVAKLLKDDFLQQNSYSSYDRFCPFYKTVGMLRNIIDFYDMARHSVESTAQSENKITWNVIREAMGNIMYQLSSMKFKDPVKDGEAKIKADFEQLHEDLQQAFRNLED

Target ORF information:

RefSeq Version	NM_001273496.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 68 kDa subunit 2, transcript variant F (Vha68-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273496.1

ORF Insert Sequence:

ATGTCCAACC TTAAGCGTTT CGATGATGAG GAGCGTGAGT CCAAATATGG ACGTGTCTTC 
GCTGTCTCCG GTCCTGTCGT CACCGCCGAG GCCATGTCTG GATCAGCTAT GTACGAGTTG 
GTCCGCGTCG GCTACTACGA GCTGGTGGGC GAGATCATCC GTCTGGAGGG TGACATGGCC 
ACCATCCAGG TGTACGAGGA GACCTCTGGC GTAACTGTCG GAGATCCGGT GCTGCGTACC 
GGCAAGCCTC TTTCCGTGGA GCTGGGACCC GGTATCATGG GCAGCATCTT TGACGGTATC 
CAGCGTCCCC TGAAGGACAT TAACGAGCTG ACCGAATCCA TCTACATCCC CAAGGGTGTG 
AACGTGCCCA GTTTGTCCCG CGTGGCCAGC TGGGAGTTCA ACCCCCTGAA CGTCAAGGTC 
GGCTCCCACA TCACCGGAGG TGACCTGTAC GGTCTGGTGC ATGAGAACAC TCTGGTCAAG 
CACAAGATGA TTGTGAACCC CCGCGCCAAG GGAACAGTGC GCTACATCGC CCCCTCCGGC 
AACTACAAGG TCGACGATGT CGTCCTGGAG ACCGAGTTCG ATGGAGAGAT CACCAAGCAC 
ACCATGTTGC AGGTGTGGCC AGTGCGTCAG CCACGTCCCG TGACCGAGAA GCTGCCCGCC 
AACCACCCCC TGCTCACCGG ACAGCGTGTG CTCGACTCGC TCTTCCCCTG TGTCCAGGGC 
GGTACCACCG CCATTCCCGG AGCTTTCGGT TGCGGCAAGA CTGTGATCTC GCAGGCTCTG 
TCCAAGTACT CCAACTCCGA TGTCATCATC TACGTCGGTT GCGGTGAGCG TGGTAACGAG 
ATGTCTGAGG TACTGCGTGA CTTCCCCGAG CTGTCCGTGG AGATCGACGG TGTCACCGAG 
TCCATCATGA AGCGTACCGC CCTTGTGGCC AACACCTCCA ACATGCCTGT GGCTGCTCGT 
GAGGCCTCCA TCTACACTGG TATCACCTTG TCCGAATACT TCCGTGATAT GGGTTACAAC 
GTGTCCATGA TGGCTGATTC CACCTCCCGT TGGGCTGAGG CTCTTCGTGA AATTTCTGGT 
CGTCTCGCTG AGATGCCTGC CGATTCCGGC TACCCAGCCT ACTTGGGAGC CCGTCTGGCC 
TCCTTCTACG AGCGTGCCGG TCGCGTTAAG TGCTTGGGTA ACCCCGAGCG CGAGGGATCC 
GTGTCCATTG TCGGAGCTGT GTCTCCTCCT GGTGGTGACT TCTCCGATCC CGTGACCTCC 
GCCACTCTGG GTATCGTGCA GGTGTTCTGG GGTCTCGACA AGAAGTTGGC CCAGCGCAAG 
CACTTCCCCT CGATCAACTG GCTCATCTCC TACTCGAAGT ACATGCGTGC TCTGGATGAC 
TTCTATGACA AGAACTTCCC CGAATTCGTG CCGCTGCGTA CCAAGGTCAA GGAGATCCTG 
CAGGAGGAGG AGGATCTGTC TGAGATCGTG CAACTGGTCG GCAAGGCCTC TCTGGCCGAA 
ACCGACAAGA TCACGCTGGA GGTGGCCAAG CTGCTGAAGG ACGATTTCCT GCAGCAGAAC 
TCCTACTCCT CGTACGATCG CTTCTGCCCC TTCTACAAGA CCGTGGGCAT GTTGAGGAAC 
ATCATCGACT TCTACGACAT GGCCCGTCAC TCCGTGGAGT CTACGGCTCA GTCTGAGAAC 
AAGATCACCT GGAACGTGAT TCGTGAGGCA ATGGGCAACA TTATGTACCA GCTGTCATCC 
ATGAAGTTCA AGGACCCCGT TAAGGATGGT GAGGCCAAGA TCAAGGCTGA CTTCGAGCAG 
CTGCACGAGG ACCTGCAGCA GGCCTTCAGA AATCTGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vha68-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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