sub cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sub
Entrez Gene ID	44870
Full Name	subito
Synonyms	CG12298,DmKlp54E,DmSub,Dmel\CG12298,Dub,KIF 20A,KIF20A/B,Mklp2,SUB,Sub,mei-1794
General protein information	Preferred Names subito Names CG12298 gene product from transcript CG12298-RA CG12298-PA CG12298-PB Kinesin_A double or nothing double-or-nothing sub-PA sub-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 54E7-54E7\|2-86 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299619.1	NP_001286548.1	subito, isoform B
NM_137416.3	NP_611260.1	subito, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006989	XP_308782.4
Danio rerio (zebrafish)	kif20ba	XP_005158585.1
Drosophila melanogaster (fruit fly)	sub	NP_611260.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Subito has undergone three single-gene transpositions and it is the nearest gene to the breakpoints of other single-gene transpositions and of two chromosomal inversions.
Title: Evidence for a gene involved in multiple and diverse rearrangements in the Drosophila genus.

Subito localizes to the spindle midzone at anaphase and is required for the localization of Polo, Incenp and Aurora B.
Title: Kinesin 6 family member Subito participates in mitotic spindle assembly and interacts with mitotic regulators.

Subito is required for establishing and/or maintaining the central spindle in Drosophila oocytes, and this substitutes for the role of centrosomes in organizing the bipolar spindle.
Title: The kinesinlike protein Subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes.

Encodes a kinesin-like protein required for meiotic spindle pole formation in oocytes.
Title: subito encodes a kinesin-like protein required for meiotic spindle pole formation in Drosophila melanogaster.

The following sub gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sub cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11381	NM_001299619.1 Latest version!	Drosophila melanogaster subito (sub), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11381	NM_137416.3 Latest version!	Drosophila melanogaster subito (sub), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sub ( NM_001299619.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286548.1 Drosophila melanogaster subito (sub), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11381
Clone ID Related Accession (Same CDS sequence)	NM_001299619.1 , NM_137416.3
Accession Version	NM_001299619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	subito, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAGCA AGGAGGTTTC GGAGGTGCCA AGGCGGGAGG TGCGCTCCTT CCTGATGGCC 
CGGGATCCCT CCATCGACCG CCGCTTTCGA CCGCGACCCA ACAAAAAGAT GCGTCTGTTT 
GACAACATTC AGGAGTCGGA GGAAGAAAGT TTTTCCGAAT ACTCGGACAC GGAATCGGAG 
TACAAGTACC AGTCGAGCGA GGCAACCGAA GGGGCCTCAT GCGCTACCAG TGCGGCAGAC 
AGCAGCAACG TGGAAACGGG GCCACAGGTC TTCCTGCGCT TGCGTCCCGT TAAGGATGCA 
TCCAAAGCCT ACATAGTCTC GGAAGAAGCA AATGTGCTGA TCACTAGCTG CAAAGTGGAC 
TCGACCAGCA ACAATGTGAA CCGCATGGAA AAACACTTTG GATTCACTTC CATCTTTGAC 
AGCACCGTTG GCCAGCGAGA CATCTATGAT ACCTGTGTGG GTCCCAAAAT TATGGAGGAG 
GAATGTGTGA CCATTATGAC ATATGGCACA TCGGGGTCTG GAAAAACGTA TACCTTACTG 
GGCGATGATG TGCGTGCTGG AATCATTCCG CGTGCCCTGG AAAACATATT CACCATTTAC 
CAGGACACCG TCTTCCGATC GCCCAAGCTG AAGCTAATCA ATGGCAGCAT CGTGTTCTTG 
CAGGACGACG CCTCGCTGAA AGAGCTGCAG ATTCGGAAAA AACTTTTAGA CTTATGTCCG 
GATATCAGTG CCCACCATCA GCGTCTGAAG CAGGTCATTG ATGGCGACCA CATGTTCGAA 
ACGAAGGCTT CCACCGATGT TTCCGTCTTG GTTTGGGTGT CCTTCGTGGA GATCTACAAT 
GAACTTGTGT ACGACCTGCT AGCCATTCCG CCGAAACAGG ACAAACTGGG GGAGGTGCCA 
CGAAAAAACC TGAAGATCGT GGGCAACAAA GGCCATGTGT TCATCAAGGG TCTGACCAGT 
GTTTTCGTGA CGAGCAGCGA GGAGGCGCTG CGTCTGCTCC GGCTGGGCCA GCAGCGCTCC 
ACGTACGCCT CCACCTCGGT GAACGCCAAC TCTAGTCGGT CGCACTGTGT CTTTACCGTG 
GACATACTAA AGTACAATCG GTCGGGCATC ACCACACAGA GTTCGTACAA ATTCTGCGAC 
CTGGCGGGTT CGGAACGCGT CAACAACACG GGTACCTCCG GCCTTCGTCT CAAGGAAGCA 
AAGAACATAA ATACGTCGCT GATGGTGCTG GGTCGATGTT TAGATGCGGC CAGTACAGTT 
CAGAAAAAGA AAAACGCCGA CATCATTCCA TATCGCGACT CCAAGCTAAC AATGCTGTTG 
CAGGCTGCTT TGCTGGGCAA GGAGAAATTG GCCATGATCG TGACAGTTAC CCCTCTGGAT 
AAATACTACG AGGAGAACCT GAACGTCTTG AACTTTGCTT CCATCGCCAA GAACATCATC 
TTCAAGGAGC CCGTAATTAA ACAGCATCGC GTCAGCTACT GTGGTTTCAT GGAATTCTCA 
AAGATGTCGA CGTGCGAGGG CGGCGACTAC ACCAAGGAAC TTGAGGACGA AAACGTGCGC 
CTGCAGTTAG AGATTGAGCA ACTGAAGTAC GATCATGTGC TGCAAATGCA GCTGCTTGAG 
GAGAAACTGC GAAGGGAACT GACTGCCACC TACCAGGAGA TTATCCAAAA CAACAAGAAG 
CAATATGAAG ACGAATGCGA GAAAAAATTA CTAATTGCGC AAAGAGAATC AGAATTTATG 
CTTTCCTCTC AACGCCGAAG GTATGAAGAA CAAATAGAAG ACCTCAAAGA TGAGATAGAG 
GAACTTAAGA ATCCTGCAAG CGACACGGAC ATTTCTGATG ATCCAAACGA ATCTAAGTCT 
CCCATCGAAA TCCTCGATGA TGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286548.1
CDS	101..1987
Translation	MDSKEVSEVPRREVRSFLMARDPSIDRRFRPRPNKKMRLFDNIQESEEESFSEYSDTESEYKYQSSEATEGASCATSAADSSNVETGPQVFLRLRPVKDASKAYIVSEEANVLITSCKVDSTSNNVNRMEKHFGFTSIFDSTVGQRDIYDTCVGPKIMEEECVTIMTYGTSGSGKTYTLLGDDVRAGIIPRALENIFTIYQDTVFRSPKLKLINGSIVFLQDDASLKELQIRKKLLDLCPDISAHHQRLKQVIDGDHMFETKASTDVSVLVWVSFVEIYNELVYDLLAIPPKQDKLGEVPRKNLKIVGNKGHVFIKGLTSVFVTSSEEALRLLRLGQQRSTYASTSVNANSSRSHCVFTVDILKYNRSGITTQSSYKFCDLAGSERVNNTGTSGLRLKEAKNINTSLMVLGRCLDAASTVQKKKNADIIPYRDSKLTMLLQAALLGKEKLAMIVTVTPLDKYYEENLNVLNFASIAKNIIFKEPVIKQHRVSYCGFMEFSKMSTCEGGDYTKELEDENVRLQLEIEQLKYDHVLQMQLLEEKLRRELTATYQEIIQNNKKQYEDECEKKLLIAQRESEFMLSSQRRRYEEQIEDLKDEIEELKNPASDTDISDDPNESKSPIEILDDD

Target ORF information:

RefSeq Version	NM_001299619.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster subito (sub), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299619.1

ORF Insert Sequence:

ATGGACAGCA AGGAGGTTTC GGAGGTGCCA AGGCGGGAGG TGCGCTCCTT CCTGATGGCC 
CGGGATCCCT CCATCGACCG CCGCTTTCGA CCGCGACCCA ACAAAAAGAT GCGTCTGTTT 
GACAACATTC AGGAGTCGGA GGAAGAAAGT TTTTCCGAAT ACTCGGACAC GGAATCGGAG 
TACAAGTACC AGTCGAGCGA GGCAACCGAA GGGGCCTCAT GCGCTACCAG TGCGGCAGAC 
AGCAGCAACG TGGAAACGGG GCCACAGGTC TTCCTGCGCT TGCGTCCCGT TAAGGATGCA 
TCCAAAGCCT ACATAGTCTC GGAAGAAGCA AATGTGCTGA TCACTAGCTG CAAAGTGGAC 
TCGACCAGCA ACAATGTGAA CCGCATGGAA AAACACTTTG GATTCACTTC CATCTTTGAC 
AGCACCGTTG GCCAGCGAGA CATCTATGAT ACCTGTGTGG GTCCCAAAAT TATGGAGGAG 
GAATGTGTGA CCATTATGAC ATATGGCACA TCGGGGTCTG GAAAAACGTA TACCTTACTG 
GGCGATGATG TGCGTGCTGG AATCATTCCG CGTGCCCTGG AAAACATATT CACCATTTAC 
CAGGACACCG TCTTCCGATC GCCCAAGCTG AAGCTAATCA ATGGCAGCAT CGTGTTCTTG 
CAGGACGACG CCTCGCTGAA AGAGCTGCAG ATTCGGAAAA AACTTTTAGA CTTATGTCCG 
GATATCAGTG CCCACCATCA GCGTCTGAAG CAGGTCATTG ATGGCGACCA CATGTTCGAA 
ACGAAGGCTT CCACCGATGT TTCCGTCTTG GTTTGGGTGT CCTTCGTGGA GATCTACAAT 
GAACTTGTGT ACGACCTGCT AGCCATTCCG CCGAAACAGG ACAAACTGGG GGAGGTGCCA 
CGAAAAAACC TGAAGATCGT GGGCAACAAA GGCCATGTGT TCATCAAGGG TCTGACCAGT 
GTTTTCGTGA CGAGCAGCGA GGAGGCGCTG CGTCTGCTCC GGCTGGGCCA GCAGCGCTCC 
ACGTACGCCT CCACCTCGGT GAACGCCAAC TCTAGTCGGT CGCACTGTGT CTTTACCGTG 
GACATACTAA AGTACAATCG GTCGGGCATC ACCACACAGA GTTCGTACAA ATTCTGCGAC 
CTGGCGGGTT CGGAACGCGT CAACAACACG GGTACCTCCG GCCTTCGTCT CAAGGAAGCA 
AAGAACATAA ATACGTCGCT GATGGTGCTG GGTCGATGTT TAGATGCGGC CAGTACAGTT 
CAGAAAAAGA AAAACGCCGA CATCATTCCA TATCGCGACT CCAAGCTAAC AATGCTGTTG 
CAGGCTGCTT TGCTGGGCAA GGAGAAATTG GCCATGATCG TGACAGTTAC CCCTCTGGAT 
AAATACTACG AGGAGAACCT GAACGTCTTG AACTTTGCTT CCATCGCCAA GAACATCATC 
TTCAAGGAGC CCGTAATTAA ACAGCATCGC GTCAGCTACT GTGGTTTCAT GGAATTCTCA 
AAGATGTCGA CGTGCGAGGG CGGCGACTAC ACCAAGGAAC TTGAGGACGA AAACGTGCGC 
CTGCAGTTAG AGATTGAGCA ACTGAAGTAC GATCATGTGC TGCAAATGCA GCTGCTTGAG 
GAGAAACTGC GAAGGGAACT GACTGCCACC TACCAGGAGA TTATCCAAAA CAACAAGAAG 
CAATATGAAG ACGAATGCGA GAAAAAATTA CTAATTGCGC AAAGAGAATC AGAATTTATG 
CTTTCCTCTC AACGCCGAAG GTATGAAGAA CAAATAGAAG ACCTCAAAGA TGAGATAGAG 
GAACTTAAGA ATCCTGCAAG CGACACGGAC ATTTCTGATG ATCCAAACGA ATCTAAGTCT 
CCCATCGAAA TCCTCGATGA TGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sub ( NM_137416.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611260.1 Drosophila melanogaster subito (sub), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11381
Clone ID Related Accession (Same CDS sequence)	NM_001299619.1 , NM_137416.3
Accession Version	NM_137416.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	subito, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137416.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAGCA AGGAGGTTTC GGAGGTGCCA AGGCGGGAGG TGCGCTCCTT CCTGATGGCC 
CGGGATCCCT CCATCGACCG CCGCTTTCGA CCGCGACCCA ACAAAAAGAT GCGTCTGTTT 
GACAACATTC AGGAGTCGGA GGAAGAAAGT TTTTCCGAAT ACTCGGACAC GGAATCGGAG 
TACAAGTACC AGTCGAGCGA GGCAACCGAA GGGGCCTCAT GCGCTACCAG TGCGGCAGAC 
AGCAGCAACG TGGAAACGGG GCCACAGGTC TTCCTGCGCT TGCGTCCCGT TAAGGATGCA 
TCCAAAGCCT ACATAGTCTC GGAAGAAGCA AATGTGCTGA TCACTAGCTG CAAAGTGGAC 
TCGACCAGCA ACAATGTGAA CCGCATGGAA AAACACTTTG GATTCACTTC CATCTTTGAC 
AGCACCGTTG GCCAGCGAGA CATCTATGAT ACCTGTGTGG GTCCCAAAAT TATGGAGGAG 
GAATGTGTGA CCATTATGAC ATATGGCACA TCGGGGTCTG GAAAAACGTA TACCTTACTG 
GGCGATGATG TGCGTGCTGG AATCATTCCG CGTGCCCTGG AAAACATATT CACCATTTAC 
CAGGACACCG TCTTCCGATC GCCCAAGCTG AAGCTAATCA ATGGCAGCAT CGTGTTCTTG 
CAGGACGACG CCTCGCTGAA AGAGCTGCAG ATTCGGAAAA AACTTTTAGA CTTATGTCCG 
GATATCAGTG CCCACCATCA GCGTCTGAAG CAGGTCATTG ATGGCGACCA CATGTTCGAA 
ACGAAGGCTT CCACCGATGT TTCCGTCTTG GTTTGGGTGT CCTTCGTGGA GATCTACAAT 
GAACTTGTGT ACGACCTGCT AGCCATTCCG CCGAAACAGG ACAAACTGGG GGAGGTGCCA 
CGAAAAAACC TGAAGATCGT GGGCAACAAA GGCCATGTGT TCATCAAGGG TCTGACCAGT 
GTTTTCGTGA CGAGCAGCGA GGAGGCGCTG CGTCTGCTCC GGCTGGGCCA GCAGCGCTCC 
ACGTACGCCT CCACCTCGGT GAACGCCAAC TCTAGTCGGT CGCACTGTGT CTTTACCGTG 
GACATACTAA AGTACAATCG GTCGGGCATC ACCACACAGA GTTCGTACAA ATTCTGCGAC 
CTGGCGGGTT CGGAACGCGT CAACAACACG GGTACCTCCG GCCTTCGTCT CAAGGAAGCA 
AAGAACATAA ATACGTCGCT GATGGTGCTG GGTCGATGTT TAGATGCGGC CAGTACAGTT 
CAGAAAAAGA AAAACGCCGA CATCATTCCA TATCGCGACT CCAAGCTAAC AATGCTGTTG 
CAGGCTGCTT TGCTGGGCAA GGAGAAATTG GCCATGATCG TGACAGTTAC CCCTCTGGAT 
AAATACTACG AGGAGAACCT GAACGTCTTG AACTTTGCTT CCATCGCCAA GAACATCATC 
TTCAAGGAGC CCGTAATTAA ACAGCATCGC GTCAGCTACT GTGGTTTCAT GGAATTCTCA 
AAGATGTCGA CGTGCGAGGG CGGCGACTAC ACCAAGGAAC TTGAGGACGA AAACGTGCGC 
CTGCAGTTAG AGATTGAGCA ACTGAAGTAC GATCATGTGC TGCAAATGCA GCTGCTTGAG 
GAGAAACTGC GAAGGGAACT GACTGCCACC TACCAGGAGA TTATCCAAAA CAACAAGAAG 
CAATATGAAG ACGAATGCGA GAAAAAATTA CTAATTGCGC AAAGAGAATC AGAATTTATG 
CTTTCCTCTC AACGCCGAAG GTATGAAGAA CAAATAGAAG ACCTCAAAGA TGAGATAGAG 
GAACTTAAGA ATCCTGCAAG CGACACGGAC ATTTCTGATG ATCCAAACGA ATCTAAGTCT 
CCCATCGAAA TCCTCGATGA TGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611260.1
CDS	101..1987
Translation	MDSKEVSEVPRREVRSFLMARDPSIDRRFRPRPNKKMRLFDNIQESEEESFSEYSDTESEYKYQSSEATEGASCATSAADSSNVETGPQVFLRLRPVKDASKAYIVSEEANVLITSCKVDSTSNNVNRMEKHFGFTSIFDSTVGQRDIYDTCVGPKIMEEECVTIMTYGTSGSGKTYTLLGDDVRAGIIPRALENIFTIYQDTVFRSPKLKLINGSIVFLQDDASLKELQIRKKLLDLCPDISAHHQRLKQVIDGDHMFETKASTDVSVLVWVSFVEIYNELVYDLLAIPPKQDKLGEVPRKNLKIVGNKGHVFIKGLTSVFVTSSEEALRLLRLGQQRSTYASTSVNANSSRSHCVFTVDILKYNRSGITTQSSYKFCDLAGSERVNNTGTSGLRLKEAKNINTSLMVLGRCLDAASTVQKKKNADIIPYRDSKLTMLLQAALLGKEKLAMIVTVTPLDKYYEENLNVLNFASIAKNIIFKEPVIKQHRVSYCGFMEFSKMSTCEGGDYTKELEDENVRLQLEIEQLKYDHVLQMQLLEEKLRRELTATYQEIIQNNKKQYEDECEKKLLIAQRESEFMLSSQRRRYEEQIEDLKDEIEELKNPASDTDISDDPNESKSPIEILDDD

Target ORF information:

RefSeq Version	NM_137416.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster subito (sub), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137416.3

ORF Insert Sequence:

ATGGACAGCA AGGAGGTTTC GGAGGTGCCA AGGCGGGAGG TGCGCTCCTT CCTGATGGCC 
CGGGATCCCT CCATCGACCG CCGCTTTCGA CCGCGACCCA ACAAAAAGAT GCGTCTGTTT 
GACAACATTC AGGAGTCGGA GGAAGAAAGT TTTTCCGAAT ACTCGGACAC GGAATCGGAG 
TACAAGTACC AGTCGAGCGA GGCAACCGAA GGGGCCTCAT GCGCTACCAG TGCGGCAGAC 
AGCAGCAACG TGGAAACGGG GCCACAGGTC TTCCTGCGCT TGCGTCCCGT TAAGGATGCA 
TCCAAAGCCT ACATAGTCTC GGAAGAAGCA AATGTGCTGA TCACTAGCTG CAAAGTGGAC 
TCGACCAGCA ACAATGTGAA CCGCATGGAA AAACACTTTG GATTCACTTC CATCTTTGAC 
AGCACCGTTG GCCAGCGAGA CATCTATGAT ACCTGTGTGG GTCCCAAAAT TATGGAGGAG 
GAATGTGTGA CCATTATGAC ATATGGCACA TCGGGGTCTG GAAAAACGTA TACCTTACTG 
GGCGATGATG TGCGTGCTGG AATCATTCCG CGTGCCCTGG AAAACATATT CACCATTTAC 
CAGGACACCG TCTTCCGATC GCCCAAGCTG AAGCTAATCA ATGGCAGCAT CGTGTTCTTG 
CAGGACGACG CCTCGCTGAA AGAGCTGCAG ATTCGGAAAA AACTTTTAGA CTTATGTCCG 
GATATCAGTG CCCACCATCA GCGTCTGAAG CAGGTCATTG ATGGCGACCA CATGTTCGAA 
ACGAAGGCTT CCACCGATGT TTCCGTCTTG GTTTGGGTGT CCTTCGTGGA GATCTACAAT 
GAACTTGTGT ACGACCTGCT AGCCATTCCG CCGAAACAGG ACAAACTGGG GGAGGTGCCA 
CGAAAAAACC TGAAGATCGT GGGCAACAAA GGCCATGTGT TCATCAAGGG TCTGACCAGT 
GTTTTCGTGA CGAGCAGCGA GGAGGCGCTG CGTCTGCTCC GGCTGGGCCA GCAGCGCTCC 
ACGTACGCCT CCACCTCGGT GAACGCCAAC TCTAGTCGGT CGCACTGTGT CTTTACCGTG 
GACATACTAA AGTACAATCG GTCGGGCATC ACCACACAGA GTTCGTACAA ATTCTGCGAC 
CTGGCGGGTT CGGAACGCGT CAACAACACG GGTACCTCCG GCCTTCGTCT CAAGGAAGCA 
AAGAACATAA ATACGTCGCT GATGGTGCTG GGTCGATGTT TAGATGCGGC CAGTACAGTT 
CAGAAAAAGA AAAACGCCGA CATCATTCCA TATCGCGACT CCAAGCTAAC AATGCTGTTG 
CAGGCTGCTT TGCTGGGCAA GGAGAAATTG GCCATGATCG TGACAGTTAC CCCTCTGGAT 
AAATACTACG AGGAGAACCT GAACGTCTTG AACTTTGCTT CCATCGCCAA GAACATCATC 
TTCAAGGAGC CCGTAATTAA ACAGCATCGC GTCAGCTACT GTGGTTTCAT GGAATTCTCA 
AAGATGTCGA CGTGCGAGGG CGGCGACTAC ACCAAGGAAC TTGAGGACGA AAACGTGCGC 
CTGCAGTTAG AGATTGAGCA ACTGAAGTAC GATCATGTGC TGCAAATGCA GCTGCTTGAG 
GAGAAACTGC GAAGGGAACT GACTGCCACC TACCAGGAGA TTATCCAAAA CAACAAGAAG 
CAATATGAAG ACGAATGCGA GAAAAAATTA CTAATTGCGC AAAGAGAATC AGAATTTATG 
CTTTCCTCTC AACGCCGAAG GTATGAAGAA CAAATAGAAG ACCTCAAAGA TGAGATAGAG 
GAACTTAAGA ATCCTGCAAG CGACACGGAC ATTTCTGATG ATCCAAACGA ATCTAAGTCT 
CCCATCGAAA TCCTCGATGA TGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sub cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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