miple2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	miple2
Entrez Gene ID	44787
Full Name	midkine and pleiotrophin 2
Synonyms	CG18321,Dmel\CG18321,Miple2
General protein information	Preferred Names midkine and pleiotrophin 2 Names CG18321 gene product from transcript CG18321-RG CG18321-PA CG18321-PD CG18321-PE CG18321-PF CG18321-PG Miple2 miple2-PA miple2-PD miple2-PE miple2-PF miple2-PG
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61B3-61B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274269.1	NP_001261198.1	midkine and pleiotrophin 2, isoform D
NM_001274270.1	NP_001261199.1	midkine and pleiotrophin 2, isoform E
NM_143739.3	NP_651996.2	midkine and pleiotrophin 2, isoform A
NM_001274272.1	NP_001261201.1	midkine and pleiotrophin 2, isoform G
NM_001274271.1	NP_001261200.1	midkine and pleiotrophin 2, isoform F

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data suggest that Alk is not activated by MDK/PTN related growth factors Miple1 and Miple2 in vivo.
Title: The Drosophila midkine/pleiotrophin homologues Miple1 and Miple2 affect adult lifespan but are dispensable for alk signaling during embryonic gut formation.

Strongly expressed in the developing midgut endoderm.
Title: Miple1 and miple2 encode a family of MK/PTN homologues in Drosophila melanogaster.

The following miple2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the miple2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19383	NM_001274271.1 Latest version!	Drosophila melanogaster midkine and pleiotrophin 2 (miple2), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa19383	NM_143739.3 Latest version!	Drosophila melanogaster midkine and pleiotrophin 2 (miple2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa19383	NM_001274272.1 Latest version!	Drosophila melanogaster midkine and pleiotrophin 2 (miple2), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa19384	NM_001274269.1 Latest version!	Drosophila melanogaster midkine and pleiotrophin 2 (miple2), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa19385	NM_001274270.1 Latest version!	Drosophila melanogaster midkine and pleiotrophin 2 (miple2), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

miple2 ( NM_143739.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651996.2 Drosophila melanogaster midkine and pleiotrophin 2 (miple2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa19383
Clone ID Related Accession (Same CDS sequence)	NM_143739.3 , NM_001274272.1 , NM_001274271.1
Accession Version	NM_143739.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	midkine and pleiotrophin 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_143739.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCTTA TACTGGCTTT AACCACGGCT CTGCACCTAG TTTTGGTGGT ATTGGCGCAC 
AATCCGCTAT CAACTCGTGG CGAAAGCCTT TCTGATGGCA TACGTCTGGT CATGGAAAAG 
AGGTCGGGCG ATGTGGTACA CATTCGTCCA GCTCGGGGCA CACTGGAGGC CGAAACAGTC 
GCATCCACAA CAAATAGTGA CACCCACCGC ACCAGGAACA ATCGTAAGCC GAACAAGCCT 
CAAGAGCACT CTCAGCAGGT TGGGGCCCAC AAGTCGGGAT CTAGAAAACT AGTGGTTGCG 
GAAAATGGCG GGGCACTGTC ATCACAACTA CCACAACCAA ATCACAAGCA AGGCCACAAG 
AACACTGATA AGCAACAACG AGGAGGGGGA TCCAAGCAGC AGCGAGGACA TAGCCAACAT 
CATGGAGGAA ATAGGAAAGG GCCTAAGGCA GCGACTCCAG AGAATGGATC GACTTGCCGA 
TACGCAAAGA GCGCTTGGTC CAATTGTGAT CACAAGACCA ACATGCGTTC ACGAGTCCTG 
TCCCTAAGGA AAGGAGAGCA AAACTGCTTG CCCACGCGGA CCATACAAAA GAAGTGCAAG 
AAAGGCTGCC GCTATGAAAA GGGTGAATGG TCTCAATGCG TCGGAGGACA AATAACCAGA 
GAGGATAAGT TGGAACCAGA GGCAACTGGC GGTAGTGATC AAAACTGCAA TCCTGTGCGT 
ACCGTCAGCA AGAAGTGTAA AGCAAATGGC AATTCCAGTG GAGGAAAACA ACACGGCCAG 
AGTCGTCGAA CAAAAGAGCA AAAGCAAAAG GATAAGGGAG CCAGTCACAT TTCGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651996.2
CDS	170..1009
Translation	MNLILALTTALHLVLVVLAHNPLSTRGESLSDGIRLVMEKRSGDVVHIRPARGTLEAETVASTTNSDTHRTRNNRKPNKPQEHSQQVGAHKSGSRKLVVAENGGALSSQLPQPNHKQGHKNTDKQQRGGGSKQQRGHSQHHGGNRKGPKAATPENGSTCRYAKSAWSNCDHKTNMRSRVLSLRKGEQNCLPTRTIQKKCKKGCRYEKGEWSQCVGGQITREDKLEPEATGGSDQNCNPVRTVSKKCKANGNSSGGKQHGQSRRTKEQKQKDKGASHISS

Target ORF information:

RefSeq Version	NM_143739.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster midkine and pleiotrophin 2 (miple2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143739.3

ORF Insert Sequence:

ATGAATCTTA TACTGGCTTT AACCACGGCT CTGCACCTAG TTTTGGTGGT ATTGGCGCAC 
AATCCGCTAT CAACTCGTGG CGAAAGCCTT TCTGATGGCA TACGTCTGGT CATGGAAAAG 
AGGTCGGGCG ATGTGGTACA CATTCGTCCA GCTCGGGGCA CACTGGAGGC CGAAACAGTC 
GCATCCACAA CAAATAGTGA CACCCACCGC ACCAGGAACA ATCGTAAGCC GAACAAGCCT 
CAAGAGCACT CTCAGCAGGT TGGGGCCCAC AAGTCGGGAT CTAGAAAACT AGTGGTTGCG 
GAAAATGGCG GGGCACTGTC ATCACAACTA CCACAACCAA ATCACAAGCA AGGCCACAAG 
AACACTGATA AGCAACAACG AGGAGGGGGA TCCAAGCAGC AGCGAGGACA TAGCCAACAT 
CATGGAGGAA ATAGGAAAGG GCCTAAGGCA GCGACTCCAG AGAATGGATC GACTTGCCGA 
TACGCAAAGA GCGCTTGGTC CAATTGTGAT CACAAGACCA ACATGCGTTC ACGAGTCCTG 
TCCCTAAGGA AAGGAGAGCA AAACTGCTTG CCCACGCGGA CCATACAAAA GAAGTGCAAG 
AAAGGCTGCC GCTATGAAAA GGGTGAATGG TCTCAATGCG TCGGAGGACA AATAACCAGA 
GAGGATAAGT TGGAACCAGA GGCAACTGGC GGTAGTGATC AAAACTGCAA TCCTGTGCGT 
ACCGTCAGCA AGAAGTGTAA AGCAAATGGC AATTCCAGTG GAGGAAAACA ACACGGCCAG 
AGTCGTCGAA CAAAAGAGCA AAAGCAAAAG GATAAGGGAG CCAGTCACAT TTCGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

miple2 ( NM_001274272.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261201.1 Drosophila melanogaster midkine and pleiotrophin 2 (miple2), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa19383
Clone ID Related Accession (Same CDS sequence)	NM_143739.3 , NM_001274272.1 , NM_001274271.1
Accession Version	NM_001274272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	midkine and pleiotrophin 2, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCTTA TACTGGCTTT AACCACGGCT CTGCACCTAG TTTTGGTGGT ATTGGCGCAC 
AATCCGCTAT CAACTCGTGG CGAAAGCCTT TCTGATGGCA TACGTCTGGT CATGGAAAAG 
AGGTCGGGCG ATGTGGTACA CATTCGTCCA GCTCGGGGCA CACTGGAGGC CGAAACAGTC 
GCATCCACAA CAAATAGTGA CACCCACCGC ACCAGGAACA ATCGTAAGCC GAACAAGCCT 
CAAGAGCACT CTCAGCAGGT TGGGGCCCAC AAGTCGGGAT CTAGAAAACT AGTGGTTGCG 
GAAAATGGCG GGGCACTGTC ATCACAACTA CCACAACCAA ATCACAAGCA AGGCCACAAG 
AACACTGATA AGCAACAACG AGGAGGGGGA TCCAAGCAGC AGCGAGGACA TAGCCAACAT 
CATGGAGGAA ATAGGAAAGG GCCTAAGGCA GCGACTCCAG AGAATGGATC GACTTGCCGA 
TACGCAAAGA GCGCTTGGTC CAATTGTGAT CACAAGACCA ACATGCGTTC ACGAGTCCTG 
TCCCTAAGGA AAGGAGAGCA AAACTGCTTG CCCACGCGGA CCATACAAAA GAAGTGCAAG 
AAAGGCTGCC GCTATGAAAA GGGTGAATGG TCTCAATGCG TCGGAGGACA AATAACCAGA 
GAGGATAAGT TGGAACCAGA GGCAACTGGC GGTAGTGATC AAAACTGCAA TCCTGTGCGT 
ACCGTCAGCA AGAAGTGTAA AGCAAATGGC AATTCCAGTG GAGGAAAACA ACACGGCCAG 
AGTCGTCGAA CAAAAGAGCA AAAGCAAAAG GATAAGGGAG CCAGTCACAT TTCGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261201.1
CDS	324..1163
Translation	MNLILALTTALHLVLVVLAHNPLSTRGESLSDGIRLVMEKRSGDVVHIRPARGTLEAETVASTTNSDTHRTRNNRKPNKPQEHSQQVGAHKSGSRKLVVAENGGALSSQLPQPNHKQGHKNTDKQQRGGGSKQQRGHSQHHGGNRKGPKAATPENGSTCRYAKSAWSNCDHKTNMRSRVLSLRKGEQNCLPTRTIQKKCKKGCRYEKGEWSQCVGGQITREDKLEPEATGGSDQNCNPVRTVSKKCKANGNSSGGKQHGQSRRTKEQKQKDKGASHISS

Target ORF information:

RefSeq Version	NM_001274272.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster midkine and pleiotrophin 2 (miple2), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274272.1

ORF Insert Sequence:

ATGAATCTTA TACTGGCTTT AACCACGGCT CTGCACCTAG TTTTGGTGGT ATTGGCGCAC 
AATCCGCTAT CAACTCGTGG CGAAAGCCTT TCTGATGGCA TACGTCTGGT CATGGAAAAG 
AGGTCGGGCG ATGTGGTACA CATTCGTCCA GCTCGGGGCA CACTGGAGGC CGAAACAGTC 
GCATCCACAA CAAATAGTGA CACCCACCGC ACCAGGAACA ATCGTAAGCC GAACAAGCCT 
CAAGAGCACT CTCAGCAGGT TGGGGCCCAC AAGTCGGGAT CTAGAAAACT AGTGGTTGCG 
GAAAATGGCG GGGCACTGTC ATCACAACTA CCACAACCAA ATCACAAGCA AGGCCACAAG 
AACACTGATA AGCAACAACG AGGAGGGGGA TCCAAGCAGC AGCGAGGACA TAGCCAACAT 
CATGGAGGAA ATAGGAAAGG GCCTAAGGCA GCGACTCCAG AGAATGGATC GACTTGCCGA 
TACGCAAAGA GCGCTTGGTC CAATTGTGAT CACAAGACCA ACATGCGTTC ACGAGTCCTG 
TCCCTAAGGA AAGGAGAGCA AAACTGCTTG CCCACGCGGA CCATACAAAA GAAGTGCAAG 
AAAGGCTGCC GCTATGAAAA GGGTGAATGG TCTCAATGCG TCGGAGGACA AATAACCAGA 
GAGGATAAGT TGGAACCAGA GGCAACTGGC GGTAGTGATC AAAACTGCAA TCCTGTGCGT 
ACCGTCAGCA AGAAGTGTAA AGCAAATGGC AATTCCAGTG GAGGAAAACA ACACGGCCAG 
AGTCGTCGAA CAAAAGAGCA AAAGCAAAAG GATAAGGGAG CCAGTCACAT TTCGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

miple2 ( NM_001274271.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261200.1 Drosophila melanogaster midkine and pleiotrophin 2 (miple2), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa19383
Clone ID Related Accession (Same CDS sequence)	NM_143739.3 , NM_001274272.1 , NM_001274271.1
Accession Version	NM_001274271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	midkine and pleiotrophin 2, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCTTA TACTGGCTTT AACCACGGCT CTGCACCTAG TTTTGGTGGT ATTGGCGCAC 
AATCCGCTAT CAACTCGTGG CGAAAGCCTT TCTGATGGCA TACGTCTGGT CATGGAAAAG 
AGGTCGGGCG ATGTGGTACA CATTCGTCCA GCTCGGGGCA CACTGGAGGC CGAAACAGTC 
GCATCCACAA CAAATAGTGA CACCCACCGC ACCAGGAACA ATCGTAAGCC GAACAAGCCT 
CAAGAGCACT CTCAGCAGGT TGGGGCCCAC AAGTCGGGAT CTAGAAAACT AGTGGTTGCG 
GAAAATGGCG GGGCACTGTC ATCACAACTA CCACAACCAA ATCACAAGCA AGGCCACAAG 
AACACTGATA AGCAACAACG AGGAGGGGGA TCCAAGCAGC AGCGAGGACA TAGCCAACAT 
CATGGAGGAA ATAGGAAAGG GCCTAAGGCA GCGACTCCAG AGAATGGATC GACTTGCCGA 
TACGCAAAGA GCGCTTGGTC CAATTGTGAT CACAAGACCA ACATGCGTTC ACGAGTCCTG 
TCCCTAAGGA AAGGAGAGCA AAACTGCTTG CCCACGCGGA CCATACAAAA GAAGTGCAAG 
AAAGGCTGCC GCTATGAAAA GGGTGAATGG TCTCAATGCG TCGGAGGACA AATAACCAGA 
GAGGATAAGT TGGAACCAGA GGCAACTGGC GGTAGTGATC AAAACTGCAA TCCTGTGCGT 
ACCGTCAGCA AGAAGTGTAA AGCAAATGGC AATTCCAGTG GAGGAAAACA ACACGGCCAG 
AGTCGTCGAA CAAAAGAGCA AAAGCAAAAG GATAAGGGAG CCAGTCACAT TTCGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261200.1
CDS	63..902
Translation	MNLILALTTALHLVLVVLAHNPLSTRGESLSDGIRLVMEKRSGDVVHIRPARGTLEAETVASTTNSDTHRTRNNRKPNKPQEHSQQVGAHKSGSRKLVVAENGGALSSQLPQPNHKQGHKNTDKQQRGGGSKQQRGHSQHHGGNRKGPKAATPENGSTCRYAKSAWSNCDHKTNMRSRVLSLRKGEQNCLPTRTIQKKCKKGCRYEKGEWSQCVGGQITREDKLEPEATGGSDQNCNPVRTVSKKCKANGNSSGGKQHGQSRRTKEQKQKDKGASHISS

Target ORF information:

RefSeq Version	NM_001274271.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster midkine and pleiotrophin 2 (miple2), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274271.1

ORF Insert Sequence:

ATGAATCTTA TACTGGCTTT AACCACGGCT CTGCACCTAG TTTTGGTGGT ATTGGCGCAC 
AATCCGCTAT CAACTCGTGG CGAAAGCCTT TCTGATGGCA TACGTCTGGT CATGGAAAAG 
AGGTCGGGCG ATGTGGTACA CATTCGTCCA GCTCGGGGCA CACTGGAGGC CGAAACAGTC 
GCATCCACAA CAAATAGTGA CACCCACCGC ACCAGGAACA ATCGTAAGCC GAACAAGCCT 
CAAGAGCACT CTCAGCAGGT TGGGGCCCAC AAGTCGGGAT CTAGAAAACT AGTGGTTGCG 
GAAAATGGCG GGGCACTGTC ATCACAACTA CCACAACCAA ATCACAAGCA AGGCCACAAG 
AACACTGATA AGCAACAACG AGGAGGGGGA TCCAAGCAGC AGCGAGGACA TAGCCAACAT 
CATGGAGGAA ATAGGAAAGG GCCTAAGGCA GCGACTCCAG AGAATGGATC GACTTGCCGA 
TACGCAAAGA GCGCTTGGTC CAATTGTGAT CACAAGACCA ACATGCGTTC ACGAGTCCTG 
TCCCTAAGGA AAGGAGAGCA AAACTGCTTG CCCACGCGGA CCATACAAAA GAAGTGCAAG 
AAAGGCTGCC GCTATGAAAA GGGTGAATGG TCTCAATGCG TCGGAGGACA AATAACCAGA 
GAGGATAAGT TGGAACCAGA GGCAACTGGC GGTAGTGATC AAAACTGCAA TCCTGTGCGT 
ACCGTCAGCA AGAAGTGTAA AGCAAATGGC AATTCCAGTG GAGGAAAACA ACACGGCCAG 
AGTCGTCGAA CAAAAGAGCA AAAGCAAAAG GATAAGGGAG CCAGTCACAT TTCGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

miple2 ( NM_001274269.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261198.1 Drosophila melanogaster midkine and pleiotrophin 2 (miple2), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa19384
Clone ID Related Accession (Same CDS sequence)	NM_001274269.1
Accession Version	NM_001274269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	midkine and pleiotrophin 2, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCTTA TACTGGCTTT AACCACGGCT CTGCACCTAG TTTTGGTGGT ATTGGCGCAC 
AATCCGCTAT CAACTCGTGG CGAAAGCCTT TCTGATGGCA TACGTCTGGT CATGGAAAAG 
AGGTCGGGCG ATGTGGTACA CATTCGTCCA GCTCGGGGCA CACTGGAGGC CGAAACAGTC 
GCATCCACAA CAAATAGTGA CACCCACCGC ACCAGGAACA ATCGTAAGCC GAACAAGCCT 
CAAGAGCACT CTCAGCAGGT TGGGGCCCAC AAGTCGGGAT CTAGAAAACT AGTGGTTGCG 
GAAAATGGCG GGGCACTGTC ATCACAACTA CCACAACCAA ATCACAAGCA AGGCCACAAG 
AACACTGATA AGCAACAACG AGGAGGGGGA TCCAAGCAGC AGCGAGGACA TAGCCAACAT 
CATGGAGGAA ATAGGAAAGG GCCTAAGGCA GCGACTCCAG AGAATGGATC GACTTGCCGA 
TACGCAAAGA GCGCTTGGTC CAATTGTGAT CACAAGACCA ACATGCGTTC ACGAGTCCTG 
TCCCTAAGGA AAGGAGAGCA AAACTGCTTG CCCACGCGGA CCATACAAAA GAAGTGCAAG 
AAAGGTGCGC GCGGCTGCCG CTATGAAAAG GGTGAATGGT CTCAATGCGT CGGAGGACAA 
ATAACCAGAG AGGATAAGTT GGAACCAGAG GCAACTGGCG GTAGTGATCA AAACTGCAAT 
CCTGTGCGTA CCGTCAGCAA GAAGTGTAAA GCAAATGGCA ATTCCAGTGG AGGAAAACAA 
CACGGCCAGA GTCGTCGAAC AAAAGAGCAA AAGCAAAAGG ATAAGGGAGC CAGTCACATT 
TCGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261198.1
CDS	170..1018
Translation	MNLILALTTALHLVLVVLAHNPLSTRGESLSDGIRLVMEKRSGDVVHIRPARGTLEAETVASTTNSDTHRTRNNRKPNKPQEHSQQVGAHKSGSRKLVVAENGGALSSQLPQPNHKQGHKNTDKQQRGGGSKQQRGHSQHHGGNRKGPKAATPENGSTCRYAKSAWSNCDHKTNMRSRVLSLRKGEQNCLPTRTIQKKCKKGARGCRYEKGEWSQCVGGQITREDKLEPEATGGSDQNCNPVRTVSKKCKANGNSSGGKQHGQSRRTKEQKQKDKGASHISS

Target ORF information:

RefSeq Version	NM_001274269.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster midkine and pleiotrophin 2 (miple2), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274269.1

ORF Insert Sequence:

ATGAATCTTA TACTGGCTTT AACCACGGCT CTGCACCTAG TTTTGGTGGT ATTGGCGCAC 
AATCCGCTAT CAACTCGTGG CGAAAGCCTT TCTGATGGCA TACGTCTGGT CATGGAAAAG 
AGGTCGGGCG ATGTGGTACA CATTCGTCCA GCTCGGGGCA CACTGGAGGC CGAAACAGTC 
GCATCCACAA CAAATAGTGA CACCCACCGC ACCAGGAACA ATCGTAAGCC GAACAAGCCT 
CAAGAGCACT CTCAGCAGGT TGGGGCCCAC AAGTCGGGAT CTAGAAAACT AGTGGTTGCG 
GAAAATGGCG GGGCACTGTC ATCACAACTA CCACAACCAA ATCACAAGCA AGGCCACAAG 
AACACTGATA AGCAACAACG AGGAGGGGGA TCCAAGCAGC AGCGAGGACA TAGCCAACAT 
CATGGAGGAA ATAGGAAAGG GCCTAAGGCA GCGACTCCAG AGAATGGATC GACTTGCCGA 
TACGCAAAGA GCGCTTGGTC CAATTGTGAT CACAAGACCA ACATGCGTTC ACGAGTCCTG 
TCCCTAAGGA AAGGAGAGCA AAACTGCTTG CCCACGCGGA CCATACAAAA GAAGTGCAAG 
AAAGGTGCGC GCGGCTGCCG CTATGAAAAG GGTGAATGGT CTCAATGCGT CGGAGGACAA 
ATAACCAGAG AGGATAAGTT GGAACCAGAG GCAACTGGCG GTAGTGATCA AAACTGCAAT 
CCTGTGCGTA CCGTCAGCAA GAAGTGTAAA GCAAATGGCA ATTCCAGTGG AGGAAAACAA 
CACGGCCAGA GTCGTCGAAC AAAAGAGCAA AAGCAAAAGG ATAAGGGAGC CAGTCACATT 
TCGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

miple2 ( NM_001274270.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261199.1 Drosophila melanogaster midkine and pleiotrophin 2 (miple2), transcript variant E, mRNA.

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CloneID	OFa19385
Clone ID Related Accession (Same CDS sequence)	NM_001274270.1
Accession Version	NM_001274270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	midkine and pleiotrophin 2, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCTTA TACTGGCTTT AACCACGGCT CTGCACCTAG TTTTGGTGGT ATTGGCGCAC 
AATCCGCTAT CAACTCGTGG CGAAAGCCTT TCTGATGGCA TACGTCTGGT CATGGAAAAG 
AGGTCGGGCG ATGTGGTACA CATTCGTCCA GCTCGGGGCA CACTGGAGGC CGAAACAGTC 
GCATCCACAA CAAATAGTGA CACCCACCGC ACCAGGAACA ATCGTAAGCC GAACAAGCCT 
CAAGAGCACT CTCAGCAGGT TGGGGCCCAC AAGTCGGGAT CTAGAAAACT AGTGGTTGCG 
GAAAATGGCG GGGCACTGTC ATCACAACTA CCACAACCAA ATCACAAGCA AGGCCACAAG 
AACACTGATA AGCAACAACG AGGAGGGGGA TCCAAGCAGC AGCGAGGACA TAGCCAACAT 
CATGGAGGAA ATAGGAAAGG GCCTAAGGCA GCGACTCCAG AGAATGGATC GACTTGCCGA 
TACGCAAAGA GCGCTTGGTC CAATTGTGAT CACAAGACCA ACATGCGTTC ACGAGTCCTG 
TCCCTAAGGA AAGGAGAGCA AAACTGCTTG CCCACGCGGA CCATACAAAA GAAGTGCAAG 
AAAGGCTGCC GCTATGAAAA GGGTGAATGG TCTCAATGCG TCGGAGGACA AATAACCAGA 
GAGGATAAGT TGGAACCAGA GGCAACTGGC GGTAGTGATC AAAACTGCAA TCCTGTGCGT 
ACCGTCAGCA AGAAGTGTAA AGCAAATGGC AATTCCAGTG GAGGAAAACA ACACGGCCAG 
AGTCGTCGAA CAAAAGAGCA AAAGCAAAAG GATAAGGGTA AAAGAGCCAG TCACATTTCG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261199.1
CDS	170..1015
Translation	MNLILALTTALHLVLVVLAHNPLSTRGESLSDGIRLVMEKRSGDVVHIRPARGTLEAETVASTTNSDTHRTRNNRKPNKPQEHSQQVGAHKSGSRKLVVAENGGALSSQLPQPNHKQGHKNTDKQQRGGGSKQQRGHSQHHGGNRKGPKAATPENGSTCRYAKSAWSNCDHKTNMRSRVLSLRKGEQNCLPTRTIQKKCKKGCRYEKGEWSQCVGGQITREDKLEPEATGGSDQNCNPVRTVSKKCKANGNSSGGKQHGQSRRTKEQKQKDKGKRASHISS

Target ORF information:

RefSeq Version	NM_001274270.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster midkine and pleiotrophin 2 (miple2), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274270.1

ORF Insert Sequence:

ATGAATCTTA TACTGGCTTT AACCACGGCT CTGCACCTAG TTTTGGTGGT ATTGGCGCAC 
AATCCGCTAT CAACTCGTGG CGAAAGCCTT TCTGATGGCA TACGTCTGGT CATGGAAAAG 
AGGTCGGGCG ATGTGGTACA CATTCGTCCA GCTCGGGGCA CACTGGAGGC CGAAACAGTC 
GCATCCACAA CAAATAGTGA CACCCACCGC ACCAGGAACA ATCGTAAGCC GAACAAGCCT 
CAAGAGCACT CTCAGCAGGT TGGGGCCCAC AAGTCGGGAT CTAGAAAACT AGTGGTTGCG 
GAAAATGGCG GGGCACTGTC ATCACAACTA CCACAACCAA ATCACAAGCA AGGCCACAAG 
AACACTGATA AGCAACAACG AGGAGGGGGA TCCAAGCAGC AGCGAGGACA TAGCCAACAT 
CATGGAGGAA ATAGGAAAGG GCCTAAGGCA GCGACTCCAG AGAATGGATC GACTTGCCGA 
TACGCAAAGA GCGCTTGGTC CAATTGTGAT CACAAGACCA ACATGCGTTC ACGAGTCCTG 
TCCCTAAGGA AAGGAGAGCA AAACTGCTTG CCCACGCGGA CCATACAAAA GAAGTGCAAG 
AAAGGCTGCC GCTATGAAAA GGGTGAATGG TCTCAATGCG TCGGAGGACA AATAACCAGA 
GAGGATAAGT TGGAACCAGA GGCAACTGGC GGTAGTGATC AAAACTGCAA TCCTGTGCGT 
ACCGTCAGCA AGAAGTGTAA AGCAAATGGC AATTCCAGTG GAGGAAAACA ACACGGCCAG 
AGTCGTCGAA CAAAAGAGCA AAAGCAAAAG GATAAGGGTA AAAGAGCCAG TCACATTTCG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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miple2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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