Mcr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mcr
Entrez Gene ID	44071
Full Name	Macroglobulin complement-related
Synonyms	CG7586,DmTEP6,Dmel\CG7586,E(br)155,TEP6,TEP6mel,Tep6,TepVI,alpha-2-Mrp,anon-28DE,dTEPVI,luf,mcr
General protein information	Preferred Names Macroglobulin complement-related Names CG7586 gene product from transcript CG7586-RA CG7586-PA E(br)155 Mcr-PA TEP Tep6 alpha-2-macroglobulin related enhancer of broad enhancer of broad 155 luftschlange macroglobulin complement related macroglobulin complement-related thiolester containing protein VI
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 28E1-28E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_079949.3	NP_524688.1	macroglobulin complement-related

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Mcr	NP_524688.1
Anopheles gambiae (African malaria mosquito)	TEP13	XP_317044.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The transmembrane protein Macroglobulin complement-related is essential for septate junction formation and epithelial barrier function in Drosophila.
Title: The transmembrane protein Macroglobulin complement-related is essential for septate junction formation and epithelial barrier function in Drosophila.

Mcr plays an essential role during embryonic development in septate junction organization and function.
Title: Macroglobulin complement-related encodes a protein required for septate junction organization and paracellular barrier function in Drosophila.

Mcr is secreted by D. melanogaster, it binds specifically to the surface of C. albicans, and that it promotes its subsequent phagocytosis. Mcr is closely related to the four Drosophila thioester proteins (Teps).
Title: Identification of Drosophila gene products required for phagocytosis of Candida albicans.

The following Mcr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mcr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16416	NM_079949.3 Latest version!	Drosophila melanogaster macroglobulin complement-related (Mcr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mcr ( NM_079949.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524688.1 Drosophila melanogaster macroglobulin complement-related (Mcr), mRNA.

Price & Availability↑

CloneID	OFa16416
Clone ID Related Accession (Same CDS sequence)	NM_079949.3
Accession Version	NM_079949.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	macroglobulin complement-related
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_079949.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGTGGC ACTTGCTGCG CGCCCTGCTC GTGGTAGCCG CCGTCTTGGA TGCACTGCAA 
CCGGCGGTCG GACAAAACGA CAACTACTAC AACCCCAACC AGAACCAGCA GAATCCCCAG 
CAGCCGCTAC TGCCCAACCA GCAGTGGGGT AACAATCCAC AGACGAATCA GTATAGCAAC 
AACAACCAAA ACTTTGGCCA AACGAATCCC AGTGATCGCC CGCCGTACAG GACCGATTCG 
GGGAGCTACA ACGATATTGC CGGGCAAGAT GACTATAATA AACGAGTGGG AGGAGGCTAT 
CAGGACAACG AGGAGCCGAG TCTGACTCGT GGAAAGTCGT CCTATAACAT CAAGGCCACC 
TTCCTGGAAT CGCTGCATTC CAGGGAGCCC ACTTACTTCA TTGTGGCATC TCGAATGGTC 
AGACCTGGTC TAATCTATCA GGTGTCCGTA TCCATTCTTC AAGCTCAGTA CCCGATAACC 
GTTCATGCCA GCATCGCGTG CGATGGTGTC CAAATCAGCG GAGACTCTAA GGATGTCAAG 
GAGGGCATAC CGGAGACGTT GCTCATGAGG ATCCCACCAA CTAGTGTGAC TGGAAGCTAT 
AAGCTTCGAG TGGAAGGTTT CTACCAGAAC GTTTTCGGTG GCCTCGCCTT CCTGAACGAA 
ACAAGACTAG ACTTCTCACA GCGCTCCATG ACGATATTTG TGCAGACCGA TAAGCCTCTT 
TATATGCAAG GGGAAACTGT GCGATTTAGG ACCATTCCGA TCACCACCGA ACTGAAGGGA 
TTTGACAATC CCGTCGATGT GTACATGCTG GATCCAAACA GGCATATTCT TAAACGTTGG 
CTATCGCGTC AATCAAACTT GGGTTCCGTT TCGCTGGAGT ACAAACTTTC GGATCAGCCC 
ACTTTTGGAG AATGGACTAT CCGTGTAATA GCTCAGGGAC AGCAAGAGGA GAGTCACTTC 
ACCGTGGAGG AATACTATCA GACCCGATTC GAAGTGAATG TTACCATGCC TGCGTACTTC 
TTCACAACGG ATCCGTTTAT CTACGGAAGG GTGATGGCTA ACTTTACCAG TGGCCTACCA 
GTTAGAGGCA ATCTCACAAT CAAGGCCACG ATCCGCCCAA TTGGATACTT CAGCAACCAA 
GTTCTAAATG AGAAGTATCG CCTGGGTCGT TCACCTTTGG AGCAAACTAA CCTATACAAT 
GAGCGATGGC GCTACAATAA TCCCAACCAG AATCCGCAAG TGCAGTATAA TGTGCCTGGA 
CAACTGCCAC AGGATGGAGC AGATCTTTCA CAGGATATTT TGTACAGGAA TCAATATGTG 
GTCGAGCGAC ACTATCAGTT CGACGAGGAG TGGCCATTCT GGGTTAGGAA ACCCGAGTAT 
CAGGACAGCA GCTACGAGGC GTGGAGTGGA ACTTATCGAA AGACCCTGCC CTACTTGCGC 
TACTTCAATG GTACCTTTGA CTTCAAGTGG CCATTGAGGG AGCTAGAGCT TCTGGTGCCC 
AATCTGGCCA ACTCTGAGGT GCTGATCACG GCCACTGTTG GTGAAAAGTT CTACGATGAG 
ATCATCAGTG GTTACTCCGT GGCCAGGGTG TATAATTCCA GTCTGAGGGT GGTATTCCTT 
GGCGATTCGC CGCAGGTCTT CAAGCCCGCC ATGCCATTTA CCACATATCT GGCTGTTGAG 
TACCATGATG GTTCGCCAAT TGATCCAAAC CTATTACGCC AGGGTTTAAT GGAAGTCTCT 
GGTTTCGTGG AGAGCCGCAA TGGTGGCAGG AGGGACTGGC CCGCCCAGCG ATTGCCTATG 
TCGCAGCAAA GCGATGGCAT TTGGGAGGTG AAGATTGACA TCAGGAATGA TCTGAACCTG 
GACGATCGCC CACAAGCAAG AGACTTCTTG AACGGCGTTC AGAACATGCG CTTGCAGGCG 
AACTTTGTGG ATCCGCGTGG TGAACGCATT CAAACGGAGC TCCTTTTGGT GTCTCACTAT 
TCGCCCAGAA ATCAGCACAT TAAGGTCACC ACCAGCACAG AGAAACCAGT GGTTGGGGAG 
TATATCATCT TCCACATCCG CACGAATTTC TACCTCGAAG AGTTCAACTA TCTCATTATG 
TCAAAGGGTG TTATCCTGGT GAATGACCGC GAAACCATTA CAGAGGGCAT TAAAACCATT 
GCTGTTGTTC TGAGTTCCGA AATGGCACCT GTGGCTACGA TTGTAGTGTG GAAGATTAAC 
CAGCAGGGAC AGGTTGTGGC CGATTCCCTT ACGTTCCCAG TTAATGGCAT CTCCCGGAAT 
AACTTTACCG TATATATCAA CAATCGCAAA GCGAGAACCG GAGAAAAAGT GGAGGTGGCT 
ATCTTTGGAG AACCGGGCTC GTATGTTGGT CTCTCTGGCA TCGACAGTGC ATTCTACACA 
ATGCAAGCCG GCAACGAGCT CACTTACGCC AAGATCATCA CTAAGATGTC CAACTTCGAC 
GAGCAGACCA ATGGAACGTA TAAGCACATT TGGTACTCTC ACGAAGGCAA TCCCGATGAG 
CTGGTGTACT TCCCCGCCTC GTCCTTTGGC GTCGATGCAA ATCGCACTTT TGAGTACAGT 
GGACTGATCG TATTTACGGA TGGTTATGTA CCCAGAAGGC AGGACACCTG CAATCGTACT 
TTGGGTTTCG GAGAGTGTTT GAGTGGACGT TGTTATCGCC TGGAGAAGCA GTGCGATGGT 
TTGTTTGATT GTGATGATGG AACGGATGAG ATCAATTGCC ATGCGCGTAA TGACACAGAG 
CTGTTGAACT ACAGAAAGTA CCGATTTAAT CGCGTGCTTC GCCATTACGA AAATGTGTGG 
CTATGGAAGG ATGTGAACAT TGGACCACAT GGTCGATACA TTTTTAATGT GGAGGTGCCT 
GATCGCCCCG CCTATTGGAT GGTCAGTGCG TTTAGTGTGA GTCCATCGAA AGGCTTTGGA 
ATGATGAACA AGGCCTTGGA GTACGTTGGT GTCCAACCAT TCTTCATAAA CGTGGAAATG 
CCGGAAGCTT GCCGACAAGG CGAGCAGGTG GGCATCCGTG TCACTGTTTT CAACTATATG 
ATAACTCCCA TCGAGGCGAT TGTCGTGCTG CACGACAGTC CGGACTACAA GTTTGTGCAC 
GTGGAGGAGG ATGGCATCGT CAGATCGTAT AACCCCAGAA CTAGCTTTGG TGAACATCAA 
TTTTTCATCT ACTTGGAGGC TCAAGGCACG ACGGTGGTTT ACGTGCCCGT TGTACCTCAG 
CGTCTAGGCA ATGTGGATGT GACCCTACAC GTGGCCACTT TACTTGGAAC AGATACCATA 
ACGCGAACAC TGCATGTAGA ATCGGATGGT CTGCCACAGT ATCGTCATCA ATCCGTGCTA 
CTGGATCTCT CTAACCGCGC CTATGTCCTA GAATACATGC ATGTTAATGT AACTCAAACG 
CCAGAAATTC CATACCAAGT GGATCGTTAC TTTGTGTACG GCTCAAATAA GGCTAGGATA 
TCCGTAGTGG GTGATGTGGT TGGTCCCATT TTCCCCACCA TGCCCGTAAA TGCCAGCTCC 
CTGCTTTCCT TGCCCATGGA GTCGGGCGAA CAGAATGCTT TCAGCTTCGC CGCCAATCTC 
TACACGATTA TGTACATGCG ATTAATTAAT CAACGCAACA AAACGCTGGA GAAGAATGCC 
TTTTACCACA TGAACATTGG CTATCAGCGG CAGTTGAGTT TTATGCGGCC CGATGGCAGC 
TTCTCACTGT TCCGTTCCGA TTGGAACAAC TCCGATTCGT CCGTCTGGTT GACTAGCTAC 
TGCTTAAGGG TGTTCCAAGA GGCCAGCTTC TACGAATGGG AGAACTTCAT CTGGATCGAT 
GCCACAATTA TTGAAAAGAA CATGAGATGG CTTCTGCAAC ACCAGACGCC ACAGGGATCC 
TTCTTCGAGG TCACGTGGCT GCCGGATAGG AAGATGAATC GCACCAACTT TGACAAGAAC 
ATCACGCTAA CGTCCCATGT ACTCATAACC TTGGCCACCG TAAAGGATAT CTCTGGCACT 
CTGGGCTCTC GAGTTGCTTT GGCCACGCAG CGTGCCTTGG CGTATATAGA ACGAAATATG 
GACTTCCTTA GACACCAGGC GCAACCTTTT GATGTGGCCA TTACAGCGTA CGCCCTGCAG 
CTTTGTAACT CTCCGATTGC CGAGGAAGTA TTCGCCATCC TGCGTCGCCA GGCCAGGACT 
ATAGGCGATT TTATGTACTG GGGTAACCAG GAAATACCTC AACCTCCACG CAAGCTGGAG 
AATCAAAAGT GGTTCTCGCT GCCCCGTCTG CCATACGAAT ATGATTCGCT GAATATAGAG 
ACAACCGCCT ACGCGCTATT GGTTTATGTG GCGCGCCGTG AGTTCTTCGT GGATCCCATT 
GTGCGATGGT TGAACTCGCA GCGCTTAAAC GACGGCGGAT GGGCATCCAC CCAGGATACC 
AGTGCTGCCC TCAAGGCTCT TGTGGAGTAC ACAGTCCGAT CTAGGCTGCG CGAGGTTTCC 
TCGTTGACCG TGGAGATCGA GGCCTCTTCG CAGGGCGGCA AAACACAAAC GCTTTACATT 
GATGACACGA ATCTGGCGAA ATTGCAGAGT ATCGAGATTC CAGATGCCTG GGGCACCATC 
AAGGTTCAGG CCAAGGGTGC TGGCTATGCC ATCCTGCAGA TGCACGTGCA GTACAATGTG 
GACATTGAGA AGTTCCAGAC CAAGCCGCCA GTGCCAGCCT TCGGACTTCA TACCAAGGCA 
ATCTTCCACG GCAGGAATCA GTCGCATATC TCCTATGTGG CTTGTCAAAA TTGGATAAAC 
CAAAACGAGT CGGAGCGCTC AGGCATGGCG GTCTTGGATG TGGCCATACC GACTGGCTAT 
TGGATACAGC AGCAAAAACT GGATACCTAT GTGCTCAGCA ACCGTGTGAG AAATCTGAGA 
AGAGCTCGTT ATCTAGAACG CAAGATTGTC TTTTACTTCG ATTATCTTGA TCATGAGGAT 
ATCTGTGTAA ACTTCACCAT TGAGCGTTGG TATCCAGTGG CCAATATGTC TCGCTATCTG 
CCGGTGCGCA TTTACGACTA CTATGCACCG GAACGATTCA ACGAATCCAT ATTCGATGCC 
CTGCCCACAT ATCTGCTGAA CATTTGTGAG GTGTGCGGCA GCTCTCAGTG TCCGTACTGC 
TCCATCTACA ACATGGGCTG GCGCGCCTCC ATGTCCATGT CCCTGCTCTT CTTCAGCGTA 
TTCATATACC TACTGCGAAG TCGCACGCAC CTGGTCCTAA ACATGATGCA ACTGCTCACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524688.1
CDS	175..5457
Translation	MMWHLLRALLVVAAVLDALQPAVGQNDNYYNPNQNQQNPQQPLLPNQQWGNNPQTNQYSNNNQNFGQTNPSDRPPYRTDSGSYNDIAGQDDYNKRVGGGYQDNEEPSLTRGKSSYNIKATFLESLHSREPTYFIVASRMVRPGLIYQVSVSILQAQYPITVHASIACDGVQISGDSKDVKEGIPETLLMRIPPTSVTGSYKLRVEGFYQNVFGGLAFLNETRLDFSQRSMTIFVQTDKPLYMQGETVRFRTIPITTELKGFDNPVDVYMLDPNRHILKRWLSRQSNLGSVSLEYKLSDQPTFGEWTIRVIAQGQQEESHFTVEEYYQTRFEVNVTMPAYFFTTDPFIYGRVMANFTSGLPVRGNLTIKATIRPIGYFSNQVLNEKYRLGRSPLEQTNLYNERWRYNNPNQNPQVQYNVPGQLPQDGADLSQDILYRNQYVVERHYQFDEEWPFWVRKPEYQDSSYEAWSGTYRKTLPYLRYFNGTFDFKWPLRELELLVPNLANSEVLITATVGEKFYDEIISGYSVARVYNSSLRVVFLGDSPQVFKPAMPFTTYLAVEYHDGSPIDPNLLRQGLMEVSGFVESRNGGRRDWPAQRLPMSQQSDGIWEVKIDIRNDLNLDDRPQARDFLNGVQNMRLQANFVDPRGERIQTELLLVSHYSPRNQHIKVTTSTEKPVVGEYIIFHIRTNFYLEEFNYLIMSKGVILVNDRETITEGIKTIAVVLSSEMAPVATIVVWKINQQGQVVADSLTFPVNGISRNNFTVYINNRKARTGEKVEVAIFGEPGSYVGLSGIDSAFYTMQAGNELTYAKIITKMSNFDEQTNGTYKHIWYSHEGNPDELVYFPASSFGVDANRTFEYSGLIVFTDGYVPRRQDTCNRTLGFGECLSGRCYRLEKQCDGLFDCDDGTDEINCHARNDTELLNYRKYRFNRVLRHYENVWLWKDVNIGPHGRYIFNVEVPDRPAYWMVSAFSVSPSKGFGMMNKALEYVGVQPFFINVEMPEACRQGEQVGIRVTVFNYMITPIEAIVVLHDSPDYKFVHVEEDGIVRSYNPRTSFGEHQFFIYLEAQGTTVVYVPVVPQRLGNVDVTLHVATLLGTDTITRTLHVESDGLPQYRHQSVLLDLSNRAYVLEYMHVNVTQTPEIPYQVDRYFVYGSNKARISVVGDVVGPIFPTMPVNASSLLSLPMESGEQNAFSFAANLYTIMYMRLINQRNKTLEKNAFYHMNIGYQRQLSFMRPDGSFSLFRSDWNNSDSSVWLTSYCLRVFQEASFYEWENFIWIDATIIEKNMRWLLQHQTPQGSFFEVTWLPDRKMNRTNFDKNITLTSHVLITLATVKDISGTLGSRVALATQRALAYIERNMDFLRHQAQPFDVAITAYALQLCNSPIAEEVFAILRRQARTIGDFMYWGNQEIPQPPRKLENQKWFSLPRLPYEYDSLNIETTAYALLVYVARREFFVDPIVRWLNSQRLNDGGWASTQDTSAALKALVEYTVRSRLREVSSLTVEIEASSQGGKTQTLYIDDTNLAKLQSIEIPDAWGTIKVQAKGAGYAILQMHVQYNVDIEKFQTKPPVPAFGLHTKAIFHGRNQSHISYVACQNWINQNESERSGMAVLDVAIPTGYWIQQQKLDTYVLSNRVRNLRRARYLERKIVFYFDYLDHEDICVNFTIERWYPVANMSRYLPVRIYDYYAPERFNESIFDALPTYLLNICEVCGSSQCPYCSIYNMGWRASMSMSLLFFSVFIYLLRSRTHLVLNMMQLLT

Target ORF information:

RefSeq Version	NM_079949.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster macroglobulin complement-related (Mcr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079949.3

ORF Insert Sequence:

ATGATGTGGC ACTTGCTGCG CGCCCTGCTC GTGGTAGCCG CCGTCTTGGA TGCACTGCAA 
CCGGCGGTCG GACAAAACGA CAACTACTAC AACCCCAACC AGAACCAGCA GAATCCCCAG 
CAGCCGCTAC TGCCCAACCA GCAGTGGGGT AACAATCCAC AGACGAATCA GTATAGCAAC 
AACAACCAAA ACTTTGGCCA AACGAATCCC AGTGATCGCC CGCCGTACAG GACCGATTCG 
GGGAGCTACA ACGATATTGC CGGGCAAGAT GACTATAATA AACGAGTGGG AGGAGGCTAT 
CAGGACAACG AGGAGCCGAG TCTGACTCGT GGAAAGTCGT CCTATAACAT CAAGGCCACC 
TTCCTGGAAT CGCTGCATTC CAGGGAGCCC ACTTACTTCA TTGTGGCATC TCGAATGGTC 
AGACCTGGTC TAATCTATCA GGTGTCCGTA TCCATTCTTC AAGCTCAGTA CCCGATAACC 
GTTCATGCCA GCATCGCGTG CGATGGTGTC CAAATCAGCG GAGACTCTAA GGATGTCAAG 
GAGGGCATAC CGGAGACGTT GCTCATGAGG ATCCCACCAA CTAGTGTGAC TGGAAGCTAT 
AAGCTTCGAG TGGAAGGTTT CTACCAGAAC GTTTTCGGTG GCCTCGCCTT CCTGAACGAA 
ACAAGACTAG ACTTCTCACA GCGCTCCATG ACGATATTTG TGCAGACCGA TAAGCCTCTT 
TATATGCAAG GGGAAACTGT GCGATTTAGG ACCATTCCGA TCACCACCGA ACTGAAGGGA 
TTTGACAATC CCGTCGATGT GTACATGCTG GATCCAAACA GGCATATTCT TAAACGTTGG 
CTATCGCGTC AATCAAACTT GGGTTCCGTT TCGCTGGAGT ACAAACTTTC GGATCAGCCC 
ACTTTTGGAG AATGGACTAT CCGTGTAATA GCTCAGGGAC AGCAAGAGGA GAGTCACTTC 
ACCGTGGAGG AATACTATCA GACCCGATTC GAAGTGAATG TTACCATGCC TGCGTACTTC 
TTCACAACGG ATCCGTTTAT CTACGGAAGG GTGATGGCTA ACTTTACCAG TGGCCTACCA 
GTTAGAGGCA ATCTCACAAT CAAGGCCACG ATCCGCCCAA TTGGATACTT CAGCAACCAA 
GTTCTAAATG AGAAGTATCG CCTGGGTCGT TCACCTTTGG AGCAAACTAA CCTATACAAT 
GAGCGATGGC GCTACAATAA TCCCAACCAG AATCCGCAAG TGCAGTATAA TGTGCCTGGA 
CAACTGCCAC AGGATGGAGC AGATCTTTCA CAGGATATTT TGTACAGGAA TCAATATGTG 
GTCGAGCGAC ACTATCAGTT CGACGAGGAG TGGCCATTCT GGGTTAGGAA ACCCGAGTAT 
CAGGACAGCA GCTACGAGGC GTGGAGTGGA ACTTATCGAA AGACCCTGCC CTACTTGCGC 
TACTTCAATG GTACCTTTGA CTTCAAGTGG CCATTGAGGG AGCTAGAGCT TCTGGTGCCC 
AATCTGGCCA ACTCTGAGGT GCTGATCACG GCCACTGTTG GTGAAAAGTT CTACGATGAG 
ATCATCAGTG GTTACTCCGT GGCCAGGGTG TATAATTCCA GTCTGAGGGT GGTATTCCTT 
GGCGATTCGC CGCAGGTCTT CAAGCCCGCC ATGCCATTTA CCACATATCT GGCTGTTGAG 
TACCATGATG GTTCGCCAAT TGATCCAAAC CTATTACGCC AGGGTTTAAT GGAAGTCTCT 
GGTTTCGTGG AGAGCCGCAA TGGTGGCAGG AGGGACTGGC CCGCCCAGCG ATTGCCTATG 
TCGCAGCAAA GCGATGGCAT TTGGGAGGTG AAGATTGACA TCAGGAATGA TCTGAACCTG 
GACGATCGCC CACAAGCAAG AGACTTCTTG AACGGCGTTC AGAACATGCG CTTGCAGGCG 
AACTTTGTGG ATCCGCGTGG TGAACGCATT CAAACGGAGC TCCTTTTGGT GTCTCACTAT 
TCGCCCAGAA ATCAGCACAT TAAGGTCACC ACCAGCACAG AGAAACCAGT GGTTGGGGAG 
TATATCATCT TCCACATCCG CACGAATTTC TACCTCGAAG AGTTCAACTA TCTCATTATG 
TCAAAGGGTG TTATCCTGGT GAATGACCGC GAAACCATTA CAGAGGGCAT TAAAACCATT 
GCTGTTGTTC TGAGTTCCGA AATGGCACCT GTGGCTACGA TTGTAGTGTG GAAGATTAAC 
CAGCAGGGAC AGGTTGTGGC CGATTCCCTT ACGTTCCCAG TTAATGGCAT CTCCCGGAAT 
AACTTTACCG TATATATCAA CAATCGCAAA GCGAGAACCG GAGAAAAAGT GGAGGTGGCT 
ATCTTTGGAG AACCGGGCTC GTATGTTGGT CTCTCTGGCA TCGACAGTGC ATTCTACACA 
ATGCAAGCCG GCAACGAGCT CACTTACGCC AAGATCATCA CTAAGATGTC CAACTTCGAC 
GAGCAGACCA ATGGAACGTA TAAGCACATT TGGTACTCTC ACGAAGGCAA TCCCGATGAG 
CTGGTGTACT TCCCCGCCTC GTCCTTTGGC GTCGATGCAA ATCGCACTTT TGAGTACAGT 
GGACTGATCG TATTTACGGA TGGTTATGTA CCCAGAAGGC AGGACACCTG CAATCGTACT 
TTGGGTTTCG GAGAGTGTTT GAGTGGACGT TGTTATCGCC TGGAGAAGCA GTGCGATGGT 
TTGTTTGATT GTGATGATGG AACGGATGAG ATCAATTGCC ATGCGCGTAA TGACACAGAG 
CTGTTGAACT ACAGAAAGTA CCGATTTAAT CGCGTGCTTC GCCATTACGA AAATGTGTGG 
CTATGGAAGG ATGTGAACAT TGGACCACAT GGTCGATACA TTTTTAATGT GGAGGTGCCT 
GATCGCCCCG CCTATTGGAT GGTCAGTGCG TTTAGTGTGA GTCCATCGAA AGGCTTTGGA 
ATGATGAACA AGGCCTTGGA GTACGTTGGT GTCCAACCAT TCTTCATAAA CGTGGAAATG 
CCGGAAGCTT GCCGACAAGG CGAGCAGGTG GGCATCCGTG TCACTGTTTT CAACTATATG 
ATAACTCCCA TCGAGGCGAT TGTCGTGCTG CACGACAGTC CGGACTACAA GTTTGTGCAC 
GTGGAGGAGG ATGGCATCGT CAGATCGTAT AACCCCAGAA CTAGCTTTGG TGAACATCAA 
TTTTTCATCT ACTTGGAGGC TCAAGGCACG ACGGTGGTTT ACGTGCCCGT TGTACCTCAG 
CGTCTAGGCA ATGTGGATGT GACCCTACAC GTGGCCACTT TACTTGGAAC AGATACCATA 
ACGCGAACAC TGCATGTAGA ATCGGATGGT CTGCCACAGT ATCGTCATCA ATCCGTGCTA 
CTGGATCTCT CTAACCGCGC CTATGTCCTA GAATACATGC ATGTTAATGT AACTCAAACG 
CCAGAAATTC CATACCAAGT GGATCGTTAC TTTGTGTACG GCTCAAATAA GGCTAGGATA 
TCCGTAGTGG GTGATGTGGT TGGTCCCATT TTCCCCACCA TGCCCGTAAA TGCCAGCTCC 
CTGCTTTCCT TGCCCATGGA GTCGGGCGAA CAGAATGCTT TCAGCTTCGC CGCCAATCTC 
TACACGATTA TGTACATGCG ATTAATTAAT CAACGCAACA AAACGCTGGA GAAGAATGCC 
TTTTACCACA TGAACATTGG CTATCAGCGG CAGTTGAGTT TTATGCGGCC CGATGGCAGC 
TTCTCACTGT TCCGTTCCGA TTGGAACAAC TCCGATTCGT CCGTCTGGTT GACTAGCTAC 
TGCTTAAGGG TGTTCCAAGA GGCCAGCTTC TACGAATGGG AGAACTTCAT CTGGATCGAT 
GCCACAATTA TTGAAAAGAA CATGAGATGG CTTCTGCAAC ACCAGACGCC ACAGGGATCC 
TTCTTCGAGG TCACGTGGCT GCCGGATAGG AAGATGAATC GCACCAACTT TGACAAGAAC 
ATCACGCTAA CGTCCCATGT ACTCATAACC TTGGCCACCG TAAAGGATAT CTCTGGCACT 
CTGGGCTCTC GAGTTGCTTT GGCCACGCAG CGTGCCTTGG CGTATATAGA ACGAAATATG 
GACTTCCTTA GACACCAGGC GCAACCTTTT GATGTGGCCA TTACAGCGTA CGCCCTGCAG 
CTTTGTAACT CTCCGATTGC CGAGGAAGTA TTCGCCATCC TGCGTCGCCA GGCCAGGACT 
ATAGGCGATT TTATGTACTG GGGTAACCAG GAAATACCTC AACCTCCACG CAAGCTGGAG 
AATCAAAAGT GGTTCTCGCT GCCCCGTCTG CCATACGAAT ATGATTCGCT GAATATAGAG 
ACAACCGCCT ACGCGCTATT GGTTTATGTG GCGCGCCGTG AGTTCTTCGT GGATCCCATT 
GTGCGATGGT TGAACTCGCA GCGCTTAAAC GACGGCGGAT GGGCATCCAC CCAGGATACC 
AGTGCTGCCC TCAAGGCTCT TGTGGAGTAC ACAGTCCGAT CTAGGCTGCG CGAGGTTTCC 
TCGTTGACCG TGGAGATCGA GGCCTCTTCG CAGGGCGGCA AAACACAAAC GCTTTACATT 
GATGACACGA ATCTGGCGAA ATTGCAGAGT ATCGAGATTC CAGATGCCTG GGGCACCATC 
AAGGTTCAGG CCAAGGGTGC TGGCTATGCC ATCCTGCAGA TGCACGTGCA GTACAATGTG 
GACATTGAGA AGTTCCAGAC CAAGCCGCCA GTGCCAGCCT TCGGACTTCA TACCAAGGCA 
ATCTTCCACG GCAGGAATCA GTCGCATATC TCCTATGTGG CTTGTCAAAA TTGGATAAAC 
CAAAACGAGT CGGAGCGCTC AGGCATGGCG GTCTTGGATG TGGCCATACC GACTGGCTAT 
TGGATACAGC AGCAAAAACT GGATACCTAT GTGCTCAGCA ACCGTGTGAG AAATCTGAGA 
AGAGCTCGTT ATCTAGAACG CAAGATTGTC TTTTACTTCG ATTATCTTGA TCATGAGGAT 
ATCTGTGTAA ACTTCACCAT TGAGCGTTGG TATCCAGTGG CCAATATGTC TCGCTATCTG 
CCGGTGCGCA TTTACGACTA CTATGCACCG GAACGATTCA ACGAATCCAT ATTCGATGCC 
CTGCCCACAT ATCTGCTGAA CATTTGTGAG GTGTGCGGCA GCTCTCAGTG TCCGTACTGC 
TCCATCTACA ACATGGGCTG GCGCGCCTCC ATGTCCATGT CCCTGCTCTT CTTCAGCGTA 
TTCATATACC TACTGCGAAG TCGCACGCAC CTGGTCCTAA ACATGATGCA ACTGCTCACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mcr cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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