msn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	msn
Entrez Gene ID	44030
Full Name	misshapen
Synonyms	6286,CG16973,DMSN,Dmel\CG16973,HGK,JNKKKK,MESR5,MSN,Msn,NIK,Nik,l(3)03349,l(3)06946,l(3)6286,l(3)j1E2,msm
General protein information	Preferred Names misshapen Names CG16973 gene product from transcript CG16973-RD CG16973-PA CG16973-PB CG16973-PC CG16973-PD CG16973-PF CG16973-PG CG16973-PH CG16973-PI NIK mishappen msn-PA msn-PB msn-PC msn-PD msn-PF msn-PG msn-PH msn-PI
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62E6-62E7

mRNA and Protein(s)

mRNA	Protein	Name
NM_206251.3	NP_995973.1	misshapen, isoform D
NM_206249.3	NP_995971.2	misshapen, isoform G
NM_001274404.1	NP_001261333.1	misshapen, isoform I
NM_206250.3	NP_995972.1	misshapen, isoform C
NM_001274402.1	NP_001261331.1	misshapen, isoform F
NM_079940.5	NP_524679.3	misshapen, isoform A
NM_206252.3	NP_995974.1	misshapen, isoform B
NM_001274403.1	NP_001261332.1	misshapen, isoform H

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TNIK	XP_005201764.1
Danio rerio (zebrafish)	tnikb	NP_001032765.2
Drosophila melanogaster (fruit fly)	msn	NP_995971.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the Misshapen-Warts-Yorkie pathway acts in enteroblasts to control niche signaling to intestinal stem cells
Title: The conserved misshapen-warts-Yorkie pathway acts in enteroblasts to regulate intestinal stem cells in Drosophila.

Misshapen's conserved role in cell migration and suggest that follicle cell planar polarity may be an emergent property of individual cell migratory behaviors within the epithelium.
Title: Misshapen decreases integrin levels to promote epithelial motility and planar polarity in Drosophila.

Targeted expression of an active form of Msn in the wing imaginal disk disrupted activation of endogenous MAD by Dpp and expression of the Dpp/MAD target gene.
Title: Smad inhibition by the Ste20 kinase Misshapen.

The msn gene functions to modulate the levels and/or distribution of Drosophila E-cadherin to promote the invasive migratory behaviour of border cells.
Title: The Ste20 kinase misshapen is essential for the invasive behaviour of ovarian epithelial cells in Drosophila.

the lamellocyte-active enhancer in th misshapen gene likely serves as a transcriptional target within a genetic auto-regulatory circuit that promotes the production of lamellocytes in immune-challenged or genetically-compromised animals
Title: Characterization of a lamellocyte transcriptional enhancer located within the misshapen gene of Drosophila melanogaster.

The following msn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the msn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19363	NM_001274404.1 Latest version!	Drosophila melanogaster misshapen (msn), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa19363	NM_206252.3 Latest version!	Drosophila melanogaster misshapen (msn), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa19364	NM_001274403.1 Latest version!	Drosophila melanogaster misshapen (msn), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa19365	NM_001274402.1 Latest version!	Drosophila melanogaster misshapen (msn), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa20455	NM_206251.3 Latest version!	Drosophila melanogaster misshapen (msn), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa20455	NM_206250.3 Latest version!	Drosophila melanogaster misshapen (msn), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa20456	NM_079940.5 Latest version!	Drosophila melanogaster misshapen (msn), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa20457	NM_206249.3 Latest version!	Drosophila melanogaster misshapen (msn), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

msn ( NM_001274404.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261333.1 Drosophila melanogaster misshapen (msn), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa19363
Clone ID Related Accession (Same CDS sequence)	NM_001274404.1 , NM_206252.3
Accession Version	NM_001274404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	misshapen, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT 
GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA 
AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA 
AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG 
CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA 
ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC 
TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC 
ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT 
GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC 
TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC 
TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC 
TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC 
AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC 
TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC 
ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG 
CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA 
CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC 
AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG 
ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA 
CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA 
CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA 
AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC 
CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC 
GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG 
CCATCGTTAC CACCAAGGAG ACCTGAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA 
TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG 
TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG 
CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC 
AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA 
TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC 
ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG 
CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA 
GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC 
ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG 
GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGTA TCAAGCGGCC 
ATTAGCTCAT CCGTGCATTC CACGCCATCG AAATCGTTTA TCGCCAGCAG CGGAAGCGGT 
GGTCTCGGTT TGGGGGGCGG TACCGTTGTG GGCGGCGTGA TTATCAGCAG CAATCACTCG 
CCCCAATCGA CGATATCGCT CGCCTCTTCG TCCTCGAATT CGCGCCAGAA TTCGCCCAAG 
AATTCGATCA GCAAGACGCG CTCCTCCAGC AGTATTACTA ATCTCTTGCA CAAATCTGCT 
TCTTCCTCCT CCGCGAACCT GCACCACCTG ACGCCCTGCT CCTCGACGTC CTCGGCCTCG 
ATCTCCAATC CGCTGCCACC GCACGCCTAT GCCCTGCAAC AGAAGCAGCG CAGTTTCCTG 
ACCTTTGGCT TCGGCGCCGG CGGCTCTGGT CCTTCGCGCC GGGAATCGCA CGTCAATGTC 
AATGTAACGC CCACCTCCCA CGAGGCGGCC AACGATACGC CCGAGATCCG TAAGTATAAG 
AAGCGCTTCA ATTCGGAAAT CCTGTGCGCA GCGCTATGGG GCGTCAACCT GCTGATTGGA 
ACGGAGAATG GCTTAATGCT GCTAGATCGA TCCGGTCAGG GCAAGGTCTA CCAGCTCATC 
TCGCGACGCC GTTTCCAGCA AATGGAGGTG CTAGAGGGCC AGAACATATT GGTCACCATA 
TCCGGCAAGA AGAACCGAGT GCGCGTCTAC TACTTGTCCT GGCTAAAGTC GAAGATCTTG 
CGCACCGACG GACTCTCCGA TCAAGTGGAG CGTCGGAACG GTTGGATCAA CGTGGGTGAT 
CTGCAAGGTG CTGTACATTT TAAGATTGTC AAATACGAGA GGATTAAGTT CCTAGTGATT 
GCATTAAAAG ACTCTATTGA AATTTATGCA TGGGCTCCCA AACCATATCA CAAATTTATG 
GCATTTAAGA ATTTTGGTGA ACTGGAACAT CGCCCGCTTT TGGTCGATCT CACCATTGAG 
GATCAGTCGA GACTGAAGGT GATCTATGGC TCCGCCGAGG GTTTCCATGC GGTTGATTTA 
GATTCAGCTG AAGTATACGA TATCTATCTT CCCAAGCATA CTCAGGGTGC AATCATTCCG 
CATTGTATTG TGGCGCTTCC CAACTCAAAT GGCATGCAAC TGCTGCTGTG CTACGACAAT 
GAGGGCGTCT ATGTGAACAC TGTGGGCCGC GTTTCCAAGA ACATTGTACT GCAGTGGGGC 
GAGATGCCCA CCTCGGTGGC CTACATCGGC ACTGGACAAA TCATGGGCTG GGGCAATAAA 
GCAATAGAGA TACGTTCCGT TGAGAGCGGC CATTTGGATG GTGTGTTCAT GCACAAAAAG 
GCGCAGCGCT TGAAATTCCT TTGCGAGCGA AACGACAAAG TATTCTTCAG CAGTGCCAAA 
GGTGCTTCAT CGTGTCAAAT CTACTTTATG ACGCTCAACA AGCCGGGCAT GGCCAATTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261333.1
CDS	332..3934
Translation	MAHQQQQQLAPSVNCSLDDIDLTALKDPAGIFELIEVVGNGTYGQVYKGRHTKTGQLAAIKVMDVTEDEEEEIKLEINVLKKYSNHRNIATYYGAFIKKSPPGKDDQLWLVMEYCGAGSVTDLVKSTKGQSLKEEWIAYICREILRGLSYLHSNKVIHRDIKGQNVLLTDNAEVKLVDFGVSAQLDRTIGRRNTFIGTPYWMAPEVIACDENPDATYDNRSDLWSLGITALEMAESQPPLCDLHPMRALFLIPRNSPPRLKSKKWSKKFHGFIDTVLVKDYHQRPYTENLLKHGFIKDQPTDRQVRIQLKDHIDRCKKRKQEKEREDYRYSGSDNDDDEPQLAGEHSSIVQAPGGDTLRRNFQQIQEGRLAAEQQQQHHLMAQAQAQAAAAHAAAQAQAQLQQQQQQAAAAAAAAAHAAQQAQQAAQQAQAQQPQANRQPKPPSRQQVEEPGPPARPPQRLIVVPDPPHANRPLPPTPKCGEPAGQTPQQQQRNSQNNFKPSLPPRRPEPQPQTAAGGQGSQQQAQPEAPPRNNRQSSGLSSSGGSASGGGGSSKPAAALPQQSNNHLGQPVNPLDPLDSSDSDSEPDEPNDRARNDGTLLASDPPKPLPGLGPVSEDANTTTPLSHGSGGPPNRPLPPTPDDDDQAGDRTLIMKRNRGGGGGGGGGGGGGGSSGVGADGTGLGTPGTRTSSVLPDLLSQASPATPPRHDKSSSEEYQAAISSSVHSTPSKSFIASSGSGGLGLGGGTVVGGVIISSNHSPQSTISLASSSSNSRQNSPKNSISKTRSSSSITNLLHKSASSSSANLHHLTPCSSTSSASISNPLPPHAYALQQKQRSFLTFGFGAGGSGPSRRESHVNVNVTPTSHEAANDTPEIRKYKKRFNSEILCAALWGVNLLIGTENGLMLLDRSGQGKVYQLISRRRFQQMEVLEGQNILVTISGKKNRVRVYYLSWLKSKILRTDGLSDQVERRNGWINVGDLQGAVHFKIVKYERIKFLVIALKDSIEIYAWAPKPYHKFMAFKNFGELEHRPLLVDLTIEDQSRLKVIYGSAEGFHAVDLDSAEVYDIYLPKHTQGAIIPHCIVALPNSNGMQLLLCYDNEGVYVNTVGRVSKNIVLQWGEMPTSVAYIGTGQIMGWGNKAIEIRSVESGHLDGVFMHKKAQRLKFLCERNDKVFFSSAKGASSCQIYFMTLNKPGMANW

Target ORF information:

RefSeq Version	NM_001274404.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster misshapen (msn), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274404.1

ORF Insert Sequence:

ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT 
GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA 
AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA 
AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG 
CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA 
ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC 
TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC 
ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT 
GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC 
TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC 
TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC 
TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC 
AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC 
TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC 
ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG 
CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA 
CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC 
AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG 
ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA 
CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA 
CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA 
AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC 
CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC 
GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG 
CCATCGTTAC CACCAAGGAG ACCTGAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA 
TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG 
TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG 
CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC 
AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA 
TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC 
ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG 
CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA 
GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC 
ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG 
GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGTA TCAAGCGGCC 
ATTAGCTCAT CCGTGCATTC CACGCCATCG AAATCGTTTA TCGCCAGCAG CGGAAGCGGT 
GGTCTCGGTT TGGGGGGCGG TACCGTTGTG GGCGGCGTGA TTATCAGCAG CAATCACTCG 
CCCCAATCGA CGATATCGCT CGCCTCTTCG TCCTCGAATT CGCGCCAGAA TTCGCCCAAG 
AATTCGATCA GCAAGACGCG CTCCTCCAGC AGTATTACTA ATCTCTTGCA CAAATCTGCT 
TCTTCCTCCT CCGCGAACCT GCACCACCTG ACGCCCTGCT CCTCGACGTC CTCGGCCTCG 
ATCTCCAATC CGCTGCCACC GCACGCCTAT GCCCTGCAAC AGAAGCAGCG CAGTTTCCTG 
ACCTTTGGCT TCGGCGCCGG CGGCTCTGGT CCTTCGCGCC GGGAATCGCA CGTCAATGTC 
AATGTAACGC CCACCTCCCA CGAGGCGGCC AACGATACGC CCGAGATCCG TAAGTATAAG 
AAGCGCTTCA ATTCGGAAAT CCTGTGCGCA GCGCTATGGG GCGTCAACCT GCTGATTGGA 
ACGGAGAATG GCTTAATGCT GCTAGATCGA TCCGGTCAGG GCAAGGTCTA CCAGCTCATC 
TCGCGACGCC GTTTCCAGCA AATGGAGGTG CTAGAGGGCC AGAACATATT GGTCACCATA 
TCCGGCAAGA AGAACCGAGT GCGCGTCTAC TACTTGTCCT GGCTAAAGTC GAAGATCTTG 
CGCACCGACG GACTCTCCGA TCAAGTGGAG CGTCGGAACG GTTGGATCAA CGTGGGTGAT 
CTGCAAGGTG CTGTACATTT TAAGATTGTC AAATACGAGA GGATTAAGTT CCTAGTGATT 
GCATTAAAAG ACTCTATTGA AATTTATGCA TGGGCTCCCA AACCATATCA CAAATTTATG 
GCATTTAAGA ATTTTGGTGA ACTGGAACAT CGCCCGCTTT TGGTCGATCT CACCATTGAG 
GATCAGTCGA GACTGAAGGT GATCTATGGC TCCGCCGAGG GTTTCCATGC GGTTGATTTA 
GATTCAGCTG AAGTATACGA TATCTATCTT CCCAAGCATA CTCAGGGTGC AATCATTCCG 
CATTGTATTG TGGCGCTTCC CAACTCAAAT GGCATGCAAC TGCTGCTGTG CTACGACAAT 
GAGGGCGTCT ATGTGAACAC TGTGGGCCGC GTTTCCAAGA ACATTGTACT GCAGTGGGGC 
GAGATGCCCA CCTCGGTGGC CTACATCGGC ACTGGACAAA TCATGGGCTG GGGCAATAAA 
GCAATAGAGA TACGTTCCGT TGAGAGCGGC CATTTGGATG GTGTGTTCAT GCACAAAAAG 
GCGCAGCGCT TGAAATTCCT TTGCGAGCGA AACGACAAAG TATTCTTCAG CAGTGCCAAA 
GGTGCTTCAT CGTGTCAAAT CTACTTTATG ACGCTCAACA AGCCGGGCAT GGCCAATTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msn ( NM_206252.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995974.1 Drosophila melanogaster misshapen (msn), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa19363
Clone ID Related Accession (Same CDS sequence)	NM_001274404.1 , NM_206252.3
Accession Version	NM_206252.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	misshapen, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206252.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT 
GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA 
AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA 
AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG 
CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA 
ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC 
TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC 
ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT 
GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC 
TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC 
TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC 
TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC 
AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC 
TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC 
ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG 
CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA 
CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC 
AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG 
ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA 
CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA 
CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA 
AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC 
CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC 
GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG 
CCATCGTTAC CACCAAGGAG ACCTGAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA 
TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG 
TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG 
CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC 
AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA 
TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC 
ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG 
CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA 
GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC 
ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG 
GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGTA TCAAGCGGCC 
ATTAGCTCAT CCGTGCATTC CACGCCATCG AAATCGTTTA TCGCCAGCAG CGGAAGCGGT 
GGTCTCGGTT TGGGGGGCGG TACCGTTGTG GGCGGCGTGA TTATCAGCAG CAATCACTCG 
CCCCAATCGA CGATATCGCT CGCCTCTTCG TCCTCGAATT CGCGCCAGAA TTCGCCCAAG 
AATTCGATCA GCAAGACGCG CTCCTCCAGC AGTATTACTA ATCTCTTGCA CAAATCTGCT 
TCTTCCTCCT CCGCGAACCT GCACCACCTG ACGCCCTGCT CCTCGACGTC CTCGGCCTCG 
ATCTCCAATC CGCTGCCACC GCACGCCTAT GCCCTGCAAC AGAAGCAGCG CAGTTTCCTG 
ACCTTTGGCT TCGGCGCCGG CGGCTCTGGT CCTTCGCGCC GGGAATCGCA CGTCAATGTC 
AATGTAACGC CCACCTCCCA CGAGGCGGCC AACGATACGC CCGAGATCCG TAAGTATAAG 
AAGCGCTTCA ATTCGGAAAT CCTGTGCGCA GCGCTATGGG GCGTCAACCT GCTGATTGGA 
ACGGAGAATG GCTTAATGCT GCTAGATCGA TCCGGTCAGG GCAAGGTCTA CCAGCTCATC 
TCGCGACGCC GTTTCCAGCA AATGGAGGTG CTAGAGGGCC AGAACATATT GGTCACCATA 
TCCGGCAAGA AGAACCGAGT GCGCGTCTAC TACTTGTCCT GGCTAAAGTC GAAGATCTTG 
CGCACCGACG GACTCTCCGA TCAAGTGGAG CGTCGGAACG GTTGGATCAA CGTGGGTGAT 
CTGCAAGGTG CTGTACATTT TAAGATTGTC AAATACGAGA GGATTAAGTT CCTAGTGATT 
GCATTAAAAG ACTCTATTGA AATTTATGCA TGGGCTCCCA AACCATATCA CAAATTTATG 
GCATTTAAGA ATTTTGGTGA ACTGGAACAT CGCCCGCTTT TGGTCGATCT CACCATTGAG 
GATCAGTCGA GACTGAAGGT GATCTATGGC TCCGCCGAGG GTTTCCATGC GGTTGATTTA 
GATTCAGCTG AAGTATACGA TATCTATCTT CCCAAGCATA CTCAGGGTGC AATCATTCCG 
CATTGTATTG TGGCGCTTCC CAACTCAAAT GGCATGCAAC TGCTGCTGTG CTACGACAAT 
GAGGGCGTCT ATGTGAACAC TGTGGGCCGC GTTTCCAAGA ACATTGTACT GCAGTGGGGC 
GAGATGCCCA CCTCGGTGGC CTACATCGGC ACTGGACAAA TCATGGGCTG GGGCAATAAA 
GCAATAGAGA TACGTTCCGT TGAGAGCGGC CATTTGGATG GTGTGTTCAT GCACAAAAAG 
GCGCAGCGCT TGAAATTCCT TTGCGAGCGA AACGACAAAG TATTCTTCAG CAGTGCCAAA 
GGTGCTTCAT CGTGTCAAAT CTACTTTATG ACGCTCAACA AGCCGGGCAT GGCCAATTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995974.1
CDS	195..3797
Translation	MAHQQQQQLAPSVNCSLDDIDLTALKDPAGIFELIEVVGNGTYGQVYKGRHTKTGQLAAIKVMDVTEDEEEEIKLEINVLKKYSNHRNIATYYGAFIKKSPPGKDDQLWLVMEYCGAGSVTDLVKSTKGQSLKEEWIAYICREILRGLSYLHSNKVIHRDIKGQNVLLTDNAEVKLVDFGVSAQLDRTIGRRNTFIGTPYWMAPEVIACDENPDATYDNRSDLWSLGITALEMAESQPPLCDLHPMRALFLIPRNSPPRLKSKKWSKKFHGFIDTVLVKDYHQRPYTENLLKHGFIKDQPTDRQVRIQLKDHIDRCKKRKQEKEREDYRYSGSDNDDDEPQLAGEHSSIVQAPGGDTLRRNFQQIQEGRLAAEQQQQHHLMAQAQAQAAAAHAAAQAQAQLQQQQQQAAAAAAAAAHAAQQAQQAAQQAQAQQPQANRQPKPPSRQQVEEPGPPARPPQRLIVVPDPPHANRPLPPTPKCGEPAGQTPQQQQRNSQNNFKPSLPPRRPEPQPQTAAGGQGSQQQAQPEAPPRNNRQSSGLSSSGGSASGGGGSSKPAAALPQQSNNHLGQPVNPLDPLDSSDSDSEPDEPNDRARNDGTLLASDPPKPLPGLGPVSEDANTTTPLSHGSGGPPNRPLPPTPDDDDQAGDRTLIMKRNRGGGGGGGGGGGGGGSSGVGADGTGLGTPGTRTSSVLPDLLSQASPATPPRHDKSSSEEYQAAISSSVHSTPSKSFIASSGSGGLGLGGGTVVGGVIISSNHSPQSTISLASSSSNSRQNSPKNSISKTRSSSSITNLLHKSASSSSANLHHLTPCSSTSSASISNPLPPHAYALQQKQRSFLTFGFGAGGSGPSRRESHVNVNVTPTSHEAANDTPEIRKYKKRFNSEILCAALWGVNLLIGTENGLMLLDRSGQGKVYQLISRRRFQQMEVLEGQNILVTISGKKNRVRVYYLSWLKSKILRTDGLSDQVERRNGWINVGDLQGAVHFKIVKYERIKFLVIALKDSIEIYAWAPKPYHKFMAFKNFGELEHRPLLVDLTIEDQSRLKVIYGSAEGFHAVDLDSAEVYDIYLPKHTQGAIIPHCIVALPNSNGMQLLLCYDNEGVYVNTVGRVSKNIVLQWGEMPTSVAYIGTGQIMGWGNKAIEIRSVESGHLDGVFMHKKAQRLKFLCERNDKVFFSSAKGASSCQIYFMTLNKPGMANW

Target ORF information:

RefSeq Version	NM_206252.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster misshapen (msn), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206252.3

ORF Insert Sequence:

ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT 
GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA 
AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA 
AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG 
CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA 
ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC 
TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC 
ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT 
GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC 
TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC 
TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC 
TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC 
AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC 
TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC 
ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG 
CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA 
CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC 
AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG 
ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA 
CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA 
CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA 
AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC 
CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC 
GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG 
CCATCGTTAC CACCAAGGAG ACCTGAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA 
TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG 
TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG 
CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC 
AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA 
TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC 
ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG 
CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA 
GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC 
ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG 
GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGTA TCAAGCGGCC 
ATTAGCTCAT CCGTGCATTC CACGCCATCG AAATCGTTTA TCGCCAGCAG CGGAAGCGGT 
GGTCTCGGTT TGGGGGGCGG TACCGTTGTG GGCGGCGTGA TTATCAGCAG CAATCACTCG 
CCCCAATCGA CGATATCGCT CGCCTCTTCG TCCTCGAATT CGCGCCAGAA TTCGCCCAAG 
AATTCGATCA GCAAGACGCG CTCCTCCAGC AGTATTACTA ATCTCTTGCA CAAATCTGCT 
TCTTCCTCCT CCGCGAACCT GCACCACCTG ACGCCCTGCT CCTCGACGTC CTCGGCCTCG 
ATCTCCAATC CGCTGCCACC GCACGCCTAT GCCCTGCAAC AGAAGCAGCG CAGTTTCCTG 
ACCTTTGGCT TCGGCGCCGG CGGCTCTGGT CCTTCGCGCC GGGAATCGCA CGTCAATGTC 
AATGTAACGC CCACCTCCCA CGAGGCGGCC AACGATACGC CCGAGATCCG TAAGTATAAG 
AAGCGCTTCA ATTCGGAAAT CCTGTGCGCA GCGCTATGGG GCGTCAACCT GCTGATTGGA 
ACGGAGAATG GCTTAATGCT GCTAGATCGA TCCGGTCAGG GCAAGGTCTA CCAGCTCATC 
TCGCGACGCC GTTTCCAGCA AATGGAGGTG CTAGAGGGCC AGAACATATT GGTCACCATA 
TCCGGCAAGA AGAACCGAGT GCGCGTCTAC TACTTGTCCT GGCTAAAGTC GAAGATCTTG 
CGCACCGACG GACTCTCCGA TCAAGTGGAG CGTCGGAACG GTTGGATCAA CGTGGGTGAT 
CTGCAAGGTG CTGTACATTT TAAGATTGTC AAATACGAGA GGATTAAGTT CCTAGTGATT 
GCATTAAAAG ACTCTATTGA AATTTATGCA TGGGCTCCCA AACCATATCA CAAATTTATG 
GCATTTAAGA ATTTTGGTGA ACTGGAACAT CGCCCGCTTT TGGTCGATCT CACCATTGAG 
GATCAGTCGA GACTGAAGGT GATCTATGGC TCCGCCGAGG GTTTCCATGC GGTTGATTTA 
GATTCAGCTG AAGTATACGA TATCTATCTT CCCAAGCATA CTCAGGGTGC AATCATTCCG 
CATTGTATTG TGGCGCTTCC CAACTCAAAT GGCATGCAAC TGCTGCTGTG CTACGACAAT 
GAGGGCGTCT ATGTGAACAC TGTGGGCCGC GTTTCCAAGA ACATTGTACT GCAGTGGGGC 
GAGATGCCCA CCTCGGTGGC CTACATCGGC ACTGGACAAA TCATGGGCTG GGGCAATAAA 
GCAATAGAGA TACGTTCCGT TGAGAGCGGC CATTTGGATG GTGTGTTCAT GCACAAAAAG 
GCGCAGCGCT TGAAATTCCT TTGCGAGCGA AACGACAAAG TATTCTTCAG CAGTGCCAAA 
GGTGCTTCAT CGTGTCAAAT CTACTTTATG ACGCTCAACA AGCCGGGCAT GGCCAATTGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msn ( NM_001274403.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261332.1 Drosophila melanogaster misshapen (msn), transcript variant H, mRNA.

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CloneID	OFa19364
Clone ID Related Accession (Same CDS sequence)	NM_001274403.1
Accession Version	NM_001274403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	misshapen, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT 
GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA 
AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA 
AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG 
CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA 
ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC 
TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC 
ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT 
GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC 
TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC 
TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC 
TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC 
AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC 
TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC 
ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG 
CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA 
CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC 
AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG 
ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA 
CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA 
CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA 
AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC 
CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC 
GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG 
CCATCGGATC ATTTGGATGT GTTAGCAGCA CAATTAAGTG AATTGGGCGT GGTCTTCTCC 
CAACAGCCAC AGCCGCAGAC GGCCGCCGGT GGTCAGGGAT CCCAGCAGCA GGCGCAGCCG 
GAGGCGCCGC CTCGCAACAA TCGCCAGTCG AGCGGTCTGT CCTCCTCCGG CGGATCGGCA 
TCCGGAGGCG GCGGTTCGTC GAAGCCCGCA GCCGCTCTGC CGCAGCAGTC GAACAATCAT 
TTGGGCCAGC CGGTGAATCC GCTGGATCCC TTGGACAGCA GCGATTCGGA CAGCGAGCCG 
GATGAGCCCA ACGATCGGGC TAGGAACGAT GGAACTCTAT TGGCCAGTGA TCCGCCCAAG 
CCACTGCCCG GCTTGGGACC CGTCTCCGAG GACGCCAACA CGACAACGCC CTTGAGTCAC 
GGCAGCGGTG GACCGCCCAA CCGCCCACTG CCGCCCACGC CCGACGACGA CGATCAGGCT 
GGCGATCGGA CTTTGATCAT GAAGCGCAAT CGAGGAGGAG GCGGTGGCGG CGGCGGCGGC 
GGAGGAGGCG GTGGCAGCAG CGGCGTGGGC GCCGATGGCA CTGGACTGGG AACTCCTGGG 
ACAAGGACCA GCAGCGTCCT GCCGGATCTA CTCAGCCAGG CATCGCCGGC AACGCCACCA 
CGCCATGATA AATCATCCAG TGAGGAGTAT CAAGCGGCCA TTAGCTCATC CGTGCATTCC 
ACGCCATCGA AATCGTTTAT CGCCAGCAGC GGAAGCGGTG GTCTCGGTTT GGGGGGCGGT 
ACCGTTGTGG GCGGCGTGAT TATCAGCAGC AATCACTCGC CCCAATCGAC GATATCGCTC 
GCCTCTTCGT CCTCGAATTC GCGCCAGAAT TCGCCCAAGA ATTCGATCAG CAAGACGCGC 
TCCTCCAGCA GTATTACTAA TCTCTTGCAC AAATCTGCTT CTTCCTCCTC CGCGAACCTG 
CACCACCTGA CGCCCTGCTC CTCGACGTCC TCGGCCTCGA TCTCCAATCC GCTGCCACCG 
CACGCCTATG CCCTGCAACA GAAGCAGCGC AGTTTCCTGA CCTTTGGCTT CGGCGCCGGC 
GGCTCTGGTC CTTCGCGCCG GGAATCGCAC GTCAATGTCA ATGTAACGCC CACCTCCCAC 
GAGGCGGCCA ACGATACGCC CGAGATCCGT AAGTATAAGA AGCGCTTCAA TTCGGAAATC 
CTGTGCGCAG CGCTATGGGG CGTCAACCTG CTGATTGGAA CGGAGAATGG CTTAATGCTG 
CTAGATCGAT CCGGTCAGGG CAAGGTCTAC CAGCTCATCT CGCGACGCCG TTTCCAGCAA 
ATGGAGGTGC TAGAGGGCCA GAACATATTG GTCACCATAT CCGGCAAGAA GAACCGAGTG 
CGCGTCTACT ACTTGTCCTG GCTAAAGTCG AAGATCTTGC GCACCGACGG ACTCTCCGAT 
CAAGTGGAGC GTCGGAACGG TTGGATCAAC GTGGGTGATC TGCAAGGTGC TGTACATTTT 
AAGATTGTCA AATACGAGAG GATTAAGTTC CTAGTGATTG CATTAAAAGA CTCTATTGAA 
ATTTATGCAT GGGCTCCCAA ACCATATCAC AAATTTATGG CATTTAAGAA TTTTGGTGAA 
CTGGAACATC GCCCGCTTTT GGTCGATCTC ACCATTGAGG ATCAGTCGAG ACTGAAGGTG 
ATCTATGGCT CCGCCGAGGG TTTCCATGCG GTTGATTTAG ATTCAGCTGA AGTATACGAT 
ATCTATCTTC CCAAGCATAC TCAGGGTGCA ATCATTCCGC ATTGTATTGT GGCGCTTCCC 
AACTCAAATG GCATGCAACT GCTGCTGTGC TACGACAATG AGGGCGTCTA TGTGAACACT 
GTGGGCCGCG TTTCCAAGAA CATTGTACTG CAGTGGGGCG AGATGCCCAC CTCGGTGGCC 
TACATCGGCA CTGGACAAAT CATGGGCTGG GGCAATAAAG CAATAGAGAT ACGTTCCGTT 
GAGAGCGGCC ATTTGGATGG TGTGTTCATG CACAAAAAGG CGCAGCGCTT GAAATTCCTT 
TGCGAGCGAA ACGACAAAGT ATTCTTCAGC AGTGCCAAAG GTGCTTCATC GTGTCAAATC 
TACTTTATGA CGCTCAACAA GCCGGGCATG GCCAATTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261332.1
CDS	195..3836
Translation	MAHQQQQQLAPSVNCSLDDIDLTALKDPAGIFELIEVVGNGTYGQVYKGRHTKTGQLAAIKVMDVTEDEEEEIKLEINVLKKYSNHRNIATYYGAFIKKSPPGKDDQLWLVMEYCGAGSVTDLVKSTKGQSLKEEWIAYICREILRGLSYLHSNKVIHRDIKGQNVLLTDNAEVKLVDFGVSAQLDRTIGRRNTFIGTPYWMAPEVIACDENPDATYDNRSDLWSLGITALEMAESQPPLCDLHPMRALFLIPRNSPPRLKSKKWSKKFHGFIDTVLVKDYHQRPYTENLLKHGFIKDQPTDRQVRIQLKDHIDRCKKRKQEKEREDYRYSGSDNDDDEPQLAGEHSSIVQAPGGDTLRRNFQQIQEGRLAAEQQQQHHLMAQAQAQAAAAHAAAQAQAQLQQQQQQAAAAAAAAAHAAQQAQQAAQQAQAQQPQANRQPKPPSRQQVEEPGPPARPPQRLIVVPDPPHANRPLPPTPKCGEPAGQTPQQQQRNSQNNFKPSDHLDVLAAQLSELGVVFSQQPQPQTAAGGQGSQQQAQPEAPPRNNRQSSGLSSSGGSASGGGGSSKPAAALPQQSNNHLGQPVNPLDPLDSSDSDSEPDEPNDRARNDGTLLASDPPKPLPGLGPVSEDANTTTPLSHGSGGPPNRPLPPTPDDDDQAGDRTLIMKRNRGGGGGGGGGGGGGGSSGVGADGTGLGTPGTRTSSVLPDLLSQASPATPPRHDKSSSEEYQAAISSSVHSTPSKSFIASSGSGGLGLGGGTVVGGVIISSNHSPQSTISLASSSSNSRQNSPKNSISKTRSSSSITNLLHKSASSSSANLHHLTPCSSTSSASISNPLPPHAYALQQKQRSFLTFGFGAGGSGPSRRESHVNVNVTPTSHEAANDTPEIRKYKKRFNSEILCAALWGVNLLIGTENGLMLLDRSGQGKVYQLISRRRFQQMEVLEGQNILVTISGKKNRVRVYYLSWLKSKILRTDGLSDQVERRNGWINVGDLQGAVHFKIVKYERIKFLVIALKDSIEIYAWAPKPYHKFMAFKNFGELEHRPLLVDLTIEDQSRLKVIYGSAEGFHAVDLDSAEVYDIYLPKHTQGAIIPHCIVALPNSNGMQLLLCYDNEGVYVNTVGRVSKNIVLQWGEMPTSVAYIGTGQIMGWGNKAIEIRSVESGHLDGVFMHKKAQRLKFLCERNDKVFFSSAKGASSCQIYFMTLNKPGMANW

Target ORF information:

RefSeq Version	NM_001274403.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster misshapen (msn), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274403.1

ORF Insert Sequence:

ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT 
GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA 
AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA 
AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG 
CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA 
ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC 
TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC 
ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT 
GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC 
TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC 
TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC 
TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC 
AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC 
TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC 
ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG 
CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA 
CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC 
AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG 
ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA 
CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA 
CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA 
AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC 
CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC 
GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG 
CCATCGGATC ATTTGGATGT GTTAGCAGCA CAATTAAGTG AATTGGGCGT GGTCTTCTCC 
CAACAGCCAC AGCCGCAGAC GGCCGCCGGT GGTCAGGGAT CCCAGCAGCA GGCGCAGCCG 
GAGGCGCCGC CTCGCAACAA TCGCCAGTCG AGCGGTCTGT CCTCCTCCGG CGGATCGGCA 
TCCGGAGGCG GCGGTTCGTC GAAGCCCGCA GCCGCTCTGC CGCAGCAGTC GAACAATCAT 
TTGGGCCAGC CGGTGAATCC GCTGGATCCC TTGGACAGCA GCGATTCGGA CAGCGAGCCG 
GATGAGCCCA ACGATCGGGC TAGGAACGAT GGAACTCTAT TGGCCAGTGA TCCGCCCAAG 
CCACTGCCCG GCTTGGGACC CGTCTCCGAG GACGCCAACA CGACAACGCC CTTGAGTCAC 
GGCAGCGGTG GACCGCCCAA CCGCCCACTG CCGCCCACGC CCGACGACGA CGATCAGGCT 
GGCGATCGGA CTTTGATCAT GAAGCGCAAT CGAGGAGGAG GCGGTGGCGG CGGCGGCGGC 
GGAGGAGGCG GTGGCAGCAG CGGCGTGGGC GCCGATGGCA CTGGACTGGG AACTCCTGGG 
ACAAGGACCA GCAGCGTCCT GCCGGATCTA CTCAGCCAGG CATCGCCGGC AACGCCACCA 
CGCCATGATA AATCATCCAG TGAGGAGTAT CAAGCGGCCA TTAGCTCATC CGTGCATTCC 
ACGCCATCGA AATCGTTTAT CGCCAGCAGC GGAAGCGGTG GTCTCGGTTT GGGGGGCGGT 
ACCGTTGTGG GCGGCGTGAT TATCAGCAGC AATCACTCGC CCCAATCGAC GATATCGCTC 
GCCTCTTCGT CCTCGAATTC GCGCCAGAAT TCGCCCAAGA ATTCGATCAG CAAGACGCGC 
TCCTCCAGCA GTATTACTAA TCTCTTGCAC AAATCTGCTT CTTCCTCCTC CGCGAACCTG 
CACCACCTGA CGCCCTGCTC CTCGACGTCC TCGGCCTCGA TCTCCAATCC GCTGCCACCG 
CACGCCTATG CCCTGCAACA GAAGCAGCGC AGTTTCCTGA CCTTTGGCTT CGGCGCCGGC 
GGCTCTGGTC CTTCGCGCCG GGAATCGCAC GTCAATGTCA ATGTAACGCC CACCTCCCAC 
GAGGCGGCCA ACGATACGCC CGAGATCCGT AAGTATAAGA AGCGCTTCAA TTCGGAAATC 
CTGTGCGCAG CGCTATGGGG CGTCAACCTG CTGATTGGAA CGGAGAATGG CTTAATGCTG 
CTAGATCGAT CCGGTCAGGG CAAGGTCTAC CAGCTCATCT CGCGACGCCG TTTCCAGCAA 
ATGGAGGTGC TAGAGGGCCA GAACATATTG GTCACCATAT CCGGCAAGAA GAACCGAGTG 
CGCGTCTACT ACTTGTCCTG GCTAAAGTCG AAGATCTTGC GCACCGACGG ACTCTCCGAT 
CAAGTGGAGC GTCGGAACGG TTGGATCAAC GTGGGTGATC TGCAAGGTGC TGTACATTTT 
AAGATTGTCA AATACGAGAG GATTAAGTTC CTAGTGATTG CATTAAAAGA CTCTATTGAA 
ATTTATGCAT GGGCTCCCAA ACCATATCAC AAATTTATGG CATTTAAGAA TTTTGGTGAA 
CTGGAACATC GCCCGCTTTT GGTCGATCTC ACCATTGAGG ATCAGTCGAG ACTGAAGGTG 
ATCTATGGCT CCGCCGAGGG TTTCCATGCG GTTGATTTAG ATTCAGCTGA AGTATACGAT 
ATCTATCTTC CCAAGCATAC TCAGGGTGCA ATCATTCCGC ATTGTATTGT GGCGCTTCCC 
AACTCAAATG GCATGCAACT GCTGCTGTGC TACGACAATG AGGGCGTCTA TGTGAACACT 
GTGGGCCGCG TTTCCAAGAA CATTGTACTG CAGTGGGGCG AGATGCCCAC CTCGGTGGCC 
TACATCGGCA CTGGACAAAT CATGGGCTGG GGCAATAAAG CAATAGAGAT ACGTTCCGTT 
GAGAGCGGCC ATTTGGATGG TGTGTTCATG CACAAAAAGG CGCAGCGCTT GAAATTCCTT 
TGCGAGCGAA ACGACAAAGT ATTCTTCAGC AGTGCCAAAG GTGCTTCATC GTGTCAAATC 
TACTTTATGA CGCTCAACAA GCCGGGCATG GCCAATTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msn ( NM_001274402.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261331.1 Drosophila melanogaster misshapen (msn), transcript variant F, mRNA.

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CloneID	OFa19365
Clone ID Related Accession (Same CDS sequence)	NM_001274402.1
Accession Version	NM_001274402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	misshapen, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT 
GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA 
AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA 
AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG 
CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA 
ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC 
TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC 
ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT 
GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC 
TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC 
TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC 
TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC 
AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC 
TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC 
ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG 
CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA 
CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC 
AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG 
ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA 
CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA 
CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA 
AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC 
CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC 
GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG 
CCATCGTTAC CACCAAGGAG ACCTGAGGAT CATTTGGATG TGTTAGCAGC ACAATTAAGT 
GAATTGGGCG TGGTCTTCTC CCAACAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA 
TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG 
TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG 
CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC 
AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA 
TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC 
ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG 
CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA ACTAGAGCAG 
AACATAAACC GCCTGCAAAA GTCCGCATCC ACATCGCAAG CCAATGTGAC ACCGTCGCGT 
CGCGGCGACG AATCCAATTT GCTGCGGGAC TGGGACTTTG ATCGTTTCTT CCCCAAGAAC 
GCAAACGGTC CGAGGGGCAG TGGACGTGGT AGCCCCACAA CCACTGCCAG CTTGAGCCGA 
AGCTCCCAGC TGAGCACGCT GAAGGTGGAT ACGAAACTGC AAAGGGCCAG TGTGGCGGAG 
GCCATCACGA GACCAGTTCC CCGGGGCTAT CAGCCGCTAA AGGCCGAACC AAGTGCCAGC 
CAAAGTATTG CCAAAGAACA AGGATCAGCA TCAGGATCCG GATCGGGATC GGGAAGTGCT 
AGTGGTAGCG GAAGCGGTGG TAGCACCAAT TCCCCAGCGC ATAAGCGCCA GGATTCGGAC 
TCCCGATTGC CAACGAATTT TGAGCGCGGT TTTCGACGCG AGAACTCGGA CTTCTTCCCG 
CTGGCCAAGA GATACTCGGC TGTGTTCAGT GGTGCAAGTG CATCCGGATC AACAGCTGGA 
TCGCCGTCAG CACAGGCTCT TCAGAGGTCG AGCGCAGTGT ACCAAAGGAA CAGCATTTAT 
AGCAGCAGCA TCAGCAGTAA GAGCAAGGAG AATGACGTGC CTGGAGCGCC AGGAGCAGCT 
GCAGCAGGAA CTGCCACTGC CAGTGCCAAG CCAGGACCAG CTGCAGCCAC AACCACTGCC 
ACCAAGGGAG CTGCTCCAAA GACCTCCAAG TCCCTGGCCA ACTTTCATTT CCTGCGACCG 
CGTCGCGAAA AGACTGAATC CGTCATTGTG CTGCAGAATG CTGCCGTTCG CGCCCAAAGG 
CTGCAGCAAC TGCAACAGCA GCAGCAGCAG CAGCAACTGC AGCAGCAACA GCAGCAGCAG 
AATCGAGGAG GAGGCGGTGG CGGCGGCGGC GGCGGAGGAG GCGGTGGCAG CAGCGGCGTG 
GGCGCCGATG GCACTGGACT GGGAACTCCT GGGACAAGGA CCAGCAGCGT CCTGCCGGAT 
CTACTCAGCC AGGCATCGCC GGCAACGCCA CCACGCCATG ATAAATCATC CAGTGAGGAG 
AAGCAGCGCA GTTTCCTGAC CTTTGGCTTC GGCGCCGGCG GCTCTGGTCC TTCGCGCCGG 
GAATCGCACG TCAATGTCAA TGTAACGCCC ACCTCCCACG AGGCGGCCAA CGATACGCCC 
GAGATCCGTA AGTATAAGAA GCGCTTCAAT TCGGAAATCC TGTGCGCAGC GCTATGGGGC 
GTCAACCTGC TGATTGGAAC GGAGAATGGC TTAATGCTGC TAGATCGATC CGGTCAGGGC 
AAGGTCTACC AGCTCATCTC GCGACGCCGT TTCCAGCAAA TGGAGGTGCT AGAGGGCCAG 
AACATATTGG TCACCATATC CGGCAAGAAG AACCGAGTGC GCGTCTACTA CTTGTCCTGG 
CTAAAGTCGA AGATCTTGCG CACCGACGGA CTCTCCGATC AAGTGGAGCG TCGGAACGGT 
TGGATCAACG TGGGTGATCT GCAAGGTGCT GTACATTTTA AGATTGTCAA ATACGAGAGG 
ATTAAGTTCC TAGTGATTGC ATTAAAAGAC TCTATTGAAA TTTATGCATG GGCTCCCAAA 
CCATATCACA AATTTATGGC ATTTAAGAAT TTTGGTGAAC TGGAACATCG CCCGCTTTTG 
GTCGATCTCA CCATTGAGGA TCAGTCGAGA CTGAAGGTGA TCTATGGCTC CGCCGAGGGT 
TTCCATGCGG TTGATTTAGA TTCAGCTGAA GTATACGATA TCTATCTTCC CAAGCATGGT 
GCAATCATTC CGCATTGTAT TGTGGCGCTT CCCAACTCAA ATGGCATGCA ACTGCTGCTG 
TGCTACGACA ATGAGGGCGT CTATGTGAAC ACTGTGGGCC GCGTTTCCAA GAACATTGTA 
CTGCAGTGGG GCGAGATGCC CACCTCGGTG GCCTACATCG GCACTGGACA AATCATGGGC 
TGGGGCAATA AAGCAATAGA GATACGTTCC GTTGAGAGCG GCCATTTGGA TGGTGTGTTC 
ATGCACAAAA AGGCGCAGCG CTTGAAATTC CTTTGCGAGC GAAACGACAA AGTATTCTTC 
AGCAGTGCCA AAGGTGCTTC ATCGTGTCAA ATCTACTTTA TGACGCTCAA CAAGCCGGGC 
ATGGCCAATT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261331.1
CDS	275..4489
Translation	MAHQQQQQLAPSVNCSLDDIDLTALKDPAGIFELIEVVGNGTYGQVYKGRHTKTGQLAAIKVMDVTEDEEEEIKLEINVLKKYSNHRNIATYYGAFIKKSPPGKDDQLWLVMEYCGAGSVTDLVKSTKGQSLKEEWIAYICREILRGLSYLHSNKVIHRDIKGQNVLLTDNAEVKLVDFGVSAQLDRTIGRRNTFIGTPYWMAPEVIACDENPDATYDNRSDLWSLGITALEMAESQPPLCDLHPMRALFLIPRNSPPRLKSKKWSKKFHGFIDTVLVKDYHQRPYTENLLKHGFIKDQPTDRQVRIQLKDHIDRCKKRKQEKEREDYRYSGSDNDDDEPQLAGEHSSIVQAPGGDTLRRNFQQIQEGRLAAEQQQQHHLMAQAQAQAAAAHAAAQAQAQLQQQQQQAAAAAAAAAHAAQQAQQAAQQAQAQQPQANRQPKPPSRQQVEEPGPPARPPQRLIVVPDPPHANRPLPPTPKCGEPAGQTPQQQQRNSQNNFKPSLPPRRPEDHLDVLAAQLSELGVVFSQQPQPQTAAGGQGSQQQAQPEAPPRNNRQSSGLSSSGGSASGGGGSSKPAAALPQQSNNHLGQPVNPLDPLDSSDSDSEPDEPNDRARNDGTLLASDPPKPLPGLGPVSEDANTTTPLSHGSGGPPNRPLPPTPDDDDQAGDRTLIMKRKLEQNINRLQKSASTSQANVTPSRRGDESNLLRDWDFDRFFPKNANGPRGSGRGSPTTTASLSRSSQLSTLKVDTKLQRASVAEAITRPVPRGYQPLKAEPSASQSIAKEQGSASGSGSGSGSASGSGSGGSTNSPAHKRQDSDSRLPTNFERGFRRENSDFFPLAKRYSAVFSGASASGSTAGSPSAQALQRSSAVYQRNSIYSSSISSKSKENDVPGAPGAAAAGTATASAKPGPAAATTTATKGAAPKTSKSLANFHFLRPRREKTESVIVLQNAAVRAQRLQQLQQQQQQQQLQQQQQQQNRGGGGGGGGGGGGGGSSGVGADGTGLGTPGTRTSSVLPDLLSQASPATPPRHDKSSSEEKQRSFLTFGFGAGGSGPSRRESHVNVNVTPTSHEAANDTPEIRKYKKRFNSEILCAALWGVNLLIGTENGLMLLDRSGQGKVYQLISRRRFQQMEVLEGQNILVTISGKKNRVRVYYLSWLKSKILRTDGLSDQVERRNGWINVGDLQGAVHFKIVKYERIKFLVIALKDSIEIYAWAPKPYHKFMAFKNFGELEHRPLLVDLTIEDQSRLKVIYGSAEGFHAVDLDSAEVYDIYLPKHGAIIPHCIVALPNSNGMQLLLCYDNEGVYVNTVGRVSKNIVLQWGEMPTSVAYIGTGQIMGWGNKAIEIRSVESGHLDGVFMHKKAQRLKFLCERNDKVFFSSAKGASSCQIYFMTLNKPGMANW

Target ORF information:

RefSeq Version	NM_001274402.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster misshapen (msn), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274402.1

ORF Insert Sequence:

ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT 
GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA 
AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA 
AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG 
CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA 
ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC 
TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC 
ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT 
GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC 
TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC 
TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC 
TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC 
AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC 
TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC 
ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG 
CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA 
CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC 
AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG 
ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA 
CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA 
CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA 
AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC 
CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC 
GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG 
CCATCGTTAC CACCAAGGAG ACCTGAGGAT CATTTGGATG TGTTAGCAGC ACAATTAAGT 
GAATTGGGCG TGGTCTTCTC CCAACAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA 
TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG 
TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG 
CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC 
AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA 
TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC 
ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG 
CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA ACTAGAGCAG 
AACATAAACC GCCTGCAAAA GTCCGCATCC ACATCGCAAG CCAATGTGAC ACCGTCGCGT 
CGCGGCGACG AATCCAATTT GCTGCGGGAC TGGGACTTTG ATCGTTTCTT CCCCAAGAAC 
GCAAACGGTC CGAGGGGCAG TGGACGTGGT AGCCCCACAA CCACTGCCAG CTTGAGCCGA 
AGCTCCCAGC TGAGCACGCT GAAGGTGGAT ACGAAACTGC AAAGGGCCAG TGTGGCGGAG 
GCCATCACGA GACCAGTTCC CCGGGGCTAT CAGCCGCTAA AGGCCGAACC AAGTGCCAGC 
CAAAGTATTG CCAAAGAACA AGGATCAGCA TCAGGATCCG GATCGGGATC GGGAAGTGCT 
AGTGGTAGCG GAAGCGGTGG TAGCACCAAT TCCCCAGCGC ATAAGCGCCA GGATTCGGAC 
TCCCGATTGC CAACGAATTT TGAGCGCGGT TTTCGACGCG AGAACTCGGA CTTCTTCCCG 
CTGGCCAAGA GATACTCGGC TGTGTTCAGT GGTGCAAGTG CATCCGGATC AACAGCTGGA 
TCGCCGTCAG CACAGGCTCT TCAGAGGTCG AGCGCAGTGT ACCAAAGGAA CAGCATTTAT 
AGCAGCAGCA TCAGCAGTAA GAGCAAGGAG AATGACGTGC CTGGAGCGCC AGGAGCAGCT 
GCAGCAGGAA CTGCCACTGC CAGTGCCAAG CCAGGACCAG CTGCAGCCAC AACCACTGCC 
ACCAAGGGAG CTGCTCCAAA GACCTCCAAG TCCCTGGCCA ACTTTCATTT CCTGCGACCG 
CGTCGCGAAA AGACTGAATC CGTCATTGTG CTGCAGAATG CTGCCGTTCG CGCCCAAAGG 
CTGCAGCAAC TGCAACAGCA GCAGCAGCAG CAGCAACTGC AGCAGCAACA GCAGCAGCAG 
AATCGAGGAG GAGGCGGTGG CGGCGGCGGC GGCGGAGGAG GCGGTGGCAG CAGCGGCGTG 
GGCGCCGATG GCACTGGACT GGGAACTCCT GGGACAAGGA CCAGCAGCGT CCTGCCGGAT 
CTACTCAGCC AGGCATCGCC GGCAACGCCA CCACGCCATG ATAAATCATC CAGTGAGGAG 
AAGCAGCGCA GTTTCCTGAC CTTTGGCTTC GGCGCCGGCG GCTCTGGTCC TTCGCGCCGG 
GAATCGCACG TCAATGTCAA TGTAACGCCC ACCTCCCACG AGGCGGCCAA CGATACGCCC 
GAGATCCGTA AGTATAAGAA GCGCTTCAAT TCGGAAATCC TGTGCGCAGC GCTATGGGGC 
GTCAACCTGC TGATTGGAAC GGAGAATGGC TTAATGCTGC TAGATCGATC CGGTCAGGGC 
AAGGTCTACC AGCTCATCTC GCGACGCCGT TTCCAGCAAA TGGAGGTGCT AGAGGGCCAG 
AACATATTGG TCACCATATC CGGCAAGAAG AACCGAGTGC GCGTCTACTA CTTGTCCTGG 
CTAAAGTCGA AGATCTTGCG CACCGACGGA CTCTCCGATC AAGTGGAGCG TCGGAACGGT 
TGGATCAACG TGGGTGATCT GCAAGGTGCT GTACATTTTA AGATTGTCAA ATACGAGAGG 
ATTAAGTTCC TAGTGATTGC ATTAAAAGAC TCTATTGAAA TTTATGCATG GGCTCCCAAA 
CCATATCACA AATTTATGGC ATTTAAGAAT TTTGGTGAAC TGGAACATCG CCCGCTTTTG 
GTCGATCTCA CCATTGAGGA TCAGTCGAGA CTGAAGGTGA TCTATGGCTC CGCCGAGGGT 
TTCCATGCGG TTGATTTAGA TTCAGCTGAA GTATACGATA TCTATCTTCC CAAGCATGGT 
GCAATCATTC CGCATTGTAT TGTGGCGCTT CCCAACTCAA ATGGCATGCA ACTGCTGCTG 
TGCTACGACA ATGAGGGCGT CTATGTGAAC ACTGTGGGCC GCGTTTCCAA GAACATTGTA 
CTGCAGTGGG GCGAGATGCC CACCTCGGTG GCCTACATCG GCACTGGACA AATCATGGGC 
TGGGGCAATA AAGCAATAGA GATACGTTCC GTTGAGAGCG GCCATTTGGA TGGTGTGTTC 
ATGCACAAAA AGGCGCAGCG CTTGAAATTC CTTTGCGAGC GAAACGACAA AGTATTCTTC 
AGCAGTGCCA AAGGTGCTTC ATCGTGTCAA ATCTACTTTA TGACGCTCAA CAAGCCGGGC 
ATGGCCAATT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msn ( NM_206251.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995973.1 Drosophila melanogaster misshapen (msn), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa20455
Clone ID Related Accession (Same CDS sequence)	NM_206251.3 , NM_206250.3
Accession Version	NM_206251.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	misshapen, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206251.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT 
GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA 
AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA 
AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG 
CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA 
ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC 
TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC 
ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT 
GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC 
TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC 
TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC 
TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC 
AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC 
TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC 
ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG 
CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA 
CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC 
AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG 
ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA 
CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA 
CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA 
AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC 
CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC 
GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG 
CCATCGTTAC CACCAAGGAG ACCTGAGGAT CATTTGGATG TGTTAGCAGC ACAATTAAGT 
GAATTGGGCG TGGTCTTCTC CCAACAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA 
TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG 
TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG 
CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC 
AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA 
TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC 
ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG 
CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA 
GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC 
ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG 
GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGAA GCAGCGCAGT 
TTCCTGACCT TTGGCTTCGG CGCCGGCGGC TCTGGTCCTT CGCGCCGGGA ATCGCACGTC 
AATGTCAATG TAACGCCCAC CTCCCACGAG GCGGCCAACG ATACGCCCGA GATCCGTAAG 
TATAAGAAGC GCTTCAATTC GGAAATCCTG TGCGCAGCGC TATGGGGCGT CAACCTGCTG 
ATTGGAACGG AGAATGGCTT AATGCTGCTA GATCGATCCG GTCAGGGCAA GGTCTACCAG 
CTCATCTCGC GACGCCGTTT CCAGCAAATG GAGGTGCTAG AGGGCCAGAA CATATTGGTC 
ACCATATCCG GCAAGAAGAA CCGAGTGCGC GTCTACTACT TGTCCTGGCT AAAGTCGAAG 
ATCTTGCGCA CCGACGGACT CTCCGATCAA GTGGAGCGTC GGAACGGTTG GATCAACGTG 
GGTGATCTGC AAGGTGCTGT ACATTTTAAG ATTGTCAAAT ACGAGAGGAT TAAGTTCCTA 
GTGATTGCAT TAAAAGACTC TATTGAAATT TATGCATGGG CTCCCAAACC ATATCACAAA 
TTTATGGCAT TTAAGAATTT TGGTGAACTG GAACATCGCC CGCTTTTGGT CGATCTCACC 
ATTGAGGATC AGTCGAGACT GAAGGTGATC TATGGCTCCG CCGAGGGTTT CCATGCGGTT 
GATTTAGATT CAGCTGAAGT ATACGATATC TATCTTCCCA AGCATACTCA GGGTGCAATC 
ATTCCGCATT GTATTGTGGC GCTTCCCAAC TCAAATGGCA TGCAACTGCT GCTGTGCTAC 
GACAATGAGG GCGTCTATGT GAACACTGTG GGCCGCGTTT CCAAGAACAT TGTACTGCAG 
TGGGGCGAGA TGCCCACCTC GGTGGCCTAC ATCGGCACTG GACAAATCAT GGGCTGGGGC 
AATAAAGCAA TAGAGATACG TTCCGTTGAG AGCGGCCATT TGGATGGTGT GTTCATGCAC 
AAAAAGGCGC AGCGCTTGAA ATTCCTTTGC GAGCGAAACG ACAAAGTATT CTTCAGCAGT 
GCCAAAGGTG CTTCATCGTG TCAAATCTAC TTTATGACGC TCAACAAGCC GGGCATGGCC 
AATTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995973.1
CDS	189..3497
Translation	MAHQQQQQLAPSVNCSLDDIDLTALKDPAGIFELIEVVGNGTYGQVYKGRHTKTGQLAAIKVMDVTEDEEEEIKLEINVLKKYSNHRNIATYYGAFIKKSPPGKDDQLWLVMEYCGAGSVTDLVKSTKGQSLKEEWIAYICREILRGLSYLHSNKVIHRDIKGQNVLLTDNAEVKLVDFGVSAQLDRTIGRRNTFIGTPYWMAPEVIACDENPDATYDNRSDLWSLGITALEMAESQPPLCDLHPMRALFLIPRNSPPRLKSKKWSKKFHGFIDTVLVKDYHQRPYTENLLKHGFIKDQPTDRQVRIQLKDHIDRCKKRKQEKEREDYRYSGSDNDDDEPQLAGEHSSIVQAPGGDTLRRNFQQIQEGRLAAEQQQQHHLMAQAQAQAAAAHAAAQAQAQLQQQQQQAAAAAAAAAHAAQQAQQAAQQAQAQQPQANRQPKPPSRQQVEEPGPPARPPQRLIVVPDPPHANRPLPPTPKCGEPAGQTPQQQQRNSQNNFKPSLPPRRPEDHLDVLAAQLSELGVVFSQQPQPQTAAGGQGSQQQAQPEAPPRNNRQSSGLSSSGGSASGGGGSSKPAAALPQQSNNHLGQPVNPLDPLDSSDSDSEPDEPNDRARNDGTLLASDPPKPLPGLGPVSEDANTTTPLSHGSGGPPNRPLPPTPDDDDQAGDRTLIMKRNRGGGGGGGGGGGGGGSSGVGADGTGLGTPGTRTSSVLPDLLSQASPATPPRHDKSSSEEKQRSFLTFGFGAGGSGPSRRESHVNVNVTPTSHEAANDTPEIRKYKKRFNSEILCAALWGVNLLIGTENGLMLLDRSGQGKVYQLISRRRFQQMEVLEGQNILVTISGKKNRVRVYYLSWLKSKILRTDGLSDQVERRNGWINVGDLQGAVHFKIVKYERIKFLVIALKDSIEIYAWAPKPYHKFMAFKNFGELEHRPLLVDLTIEDQSRLKVIYGSAEGFHAVDLDSAEVYDIYLPKHTQGAIIPHCIVALPNSNGMQLLLCYDNEGVYVNTVGRVSKNIVLQWGEMPTSVAYIGTGQIMGWGNKAIEIRSVESGHLDGVFMHKKAQRLKFLCERNDKVFFSSAKGASSCQIYFMTLNKPGMANW

Target ORF information:

RefSeq Version	NM_206251.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster misshapen (msn), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206251.3

ORF Insert Sequence:

ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT 
GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA 
AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA 
AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG 
CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA 
ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC 
TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC 
ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT 
GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC 
TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC 
TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC 
TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC 
AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC 
TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC 
ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG 
CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA 
CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC 
AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG 
ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA 
CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA 
CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA 
AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC 
CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC 
GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG 
CCATCGTTAC CACCAAGGAG ACCTGAGGAT CATTTGGATG TGTTAGCAGC ACAATTAAGT 
GAATTGGGCG TGGTCTTCTC CCAACAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA 
TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG 
TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG 
CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC 
AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA 
TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC 
ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG 
CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA 
GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC 
ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG 
GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGAA GCAGCGCAGT 
TTCCTGACCT TTGGCTTCGG CGCCGGCGGC TCTGGTCCTT CGCGCCGGGA ATCGCACGTC 
AATGTCAATG TAACGCCCAC CTCCCACGAG GCGGCCAACG ATACGCCCGA GATCCGTAAG 
TATAAGAAGC GCTTCAATTC GGAAATCCTG TGCGCAGCGC TATGGGGCGT CAACCTGCTG 
ATTGGAACGG AGAATGGCTT AATGCTGCTA GATCGATCCG GTCAGGGCAA GGTCTACCAG 
CTCATCTCGC GACGCCGTTT CCAGCAAATG GAGGTGCTAG AGGGCCAGAA CATATTGGTC 
ACCATATCCG GCAAGAAGAA CCGAGTGCGC GTCTACTACT TGTCCTGGCT AAAGTCGAAG 
ATCTTGCGCA CCGACGGACT CTCCGATCAA GTGGAGCGTC GGAACGGTTG GATCAACGTG 
GGTGATCTGC AAGGTGCTGT ACATTTTAAG ATTGTCAAAT ACGAGAGGAT TAAGTTCCTA 
GTGATTGCAT TAAAAGACTC TATTGAAATT TATGCATGGG CTCCCAAACC ATATCACAAA 
TTTATGGCAT TTAAGAATTT TGGTGAACTG GAACATCGCC CGCTTTTGGT CGATCTCACC 
ATTGAGGATC AGTCGAGACT GAAGGTGATC TATGGCTCCG CCGAGGGTTT CCATGCGGTT 
GATTTAGATT CAGCTGAAGT ATACGATATC TATCTTCCCA AGCATACTCA GGGTGCAATC 
ATTCCGCATT GTATTGTGGC GCTTCCCAAC TCAAATGGCA TGCAACTGCT GCTGTGCTAC 
GACAATGAGG GCGTCTATGT GAACACTGTG GGCCGCGTTT CCAAGAACAT TGTACTGCAG 
TGGGGCGAGA TGCCCACCTC GGTGGCCTAC ATCGGCACTG GACAAATCAT GGGCTGGGGC 
AATAAAGCAA TAGAGATACG TTCCGTTGAG AGCGGCCATT TGGATGGTGT GTTCATGCAC 
AAAAAGGCGC AGCGCTTGAA ATTCCTTTGC GAGCGAAACG ACAAAGTATT CTTCAGCAGT 
GCCAAAGGTG CTTCATCGTG TCAAATCTAC TTTATGACGC TCAACAAGCC GGGCATGGCC 
AATTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msn ( NM_206250.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995972.1 Drosophila melanogaster misshapen (msn), transcript variant C, mRNA.

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CloneID	OFa20455
Clone ID Related Accession (Same CDS sequence)	NM_206251.3 , NM_206250.3
Accession Version	NM_206250.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	misshapen, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206250.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT 
GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA 
AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA 
AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG 
CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA 
ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC 
TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC 
ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT 
GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC 
TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC 
TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC 
TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC 
AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC 
TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC 
ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG 
CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA 
CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC 
AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG 
ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA 
CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA 
CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA 
AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC 
CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC 
GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG 
CCATCGTTAC CACCAAGGAG ACCTGAGGAT CATTTGGATG TGTTAGCAGC ACAATTAAGT 
GAATTGGGCG TGGTCTTCTC CCAACAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA 
TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG 
TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG 
CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC 
AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA 
TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC 
ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG 
CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA 
GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC 
ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG 
GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGAA GCAGCGCAGT 
TTCCTGACCT TTGGCTTCGG CGCCGGCGGC TCTGGTCCTT CGCGCCGGGA ATCGCACGTC 
AATGTCAATG TAACGCCCAC CTCCCACGAG GCGGCCAACG ATACGCCCGA GATCCGTAAG 
TATAAGAAGC GCTTCAATTC GGAAATCCTG TGCGCAGCGC TATGGGGCGT CAACCTGCTG 
ATTGGAACGG AGAATGGCTT AATGCTGCTA GATCGATCCG GTCAGGGCAA GGTCTACCAG 
CTCATCTCGC GACGCCGTTT CCAGCAAATG GAGGTGCTAG AGGGCCAGAA CATATTGGTC 
ACCATATCCG GCAAGAAGAA CCGAGTGCGC GTCTACTACT TGTCCTGGCT AAAGTCGAAG 
ATCTTGCGCA CCGACGGACT CTCCGATCAA GTGGAGCGTC GGAACGGTTG GATCAACGTG 
GGTGATCTGC AAGGTGCTGT ACATTTTAAG ATTGTCAAAT ACGAGAGGAT TAAGTTCCTA 
GTGATTGCAT TAAAAGACTC TATTGAAATT TATGCATGGG CTCCCAAACC ATATCACAAA 
TTTATGGCAT TTAAGAATTT TGGTGAACTG GAACATCGCC CGCTTTTGGT CGATCTCACC 
ATTGAGGATC AGTCGAGACT GAAGGTGATC TATGGCTCCG CCGAGGGTTT CCATGCGGTT 
GATTTAGATT CAGCTGAAGT ATACGATATC TATCTTCCCA AGCATACTCA GGGTGCAATC 
ATTCCGCATT GTATTGTGGC GCTTCCCAAC TCAAATGGCA TGCAACTGCT GCTGTGCTAC 
GACAATGAGG GCGTCTATGT GAACACTGTG GGCCGCGTTT CCAAGAACAT TGTACTGCAG 
TGGGGCGAGA TGCCCACCTC GGTGGCCTAC ATCGGCACTG GACAAATCAT GGGCTGGGGC 
AATAAAGCAA TAGAGATACG TTCCGTTGAG AGCGGCCATT TGGATGGTGT GTTCATGCAC 
AAAAAGGCGC AGCGCTTGAA ATTCCTTTGC GAGCGAAACG ACAAAGTATT CTTCAGCAGT 
GCCAAAGGTG CTTCATCGTG TCAAATCTAC TTTATGACGC TCAACAAGCC GGGCATGGCC 
AATTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995972.1
CDS	178..3486
Translation	MAHQQQQQLAPSVNCSLDDIDLTALKDPAGIFELIEVVGNGTYGQVYKGRHTKTGQLAAIKVMDVTEDEEEEIKLEINVLKKYSNHRNIATYYGAFIKKSPPGKDDQLWLVMEYCGAGSVTDLVKSTKGQSLKEEWIAYICREILRGLSYLHSNKVIHRDIKGQNVLLTDNAEVKLVDFGVSAQLDRTIGRRNTFIGTPYWMAPEVIACDENPDATYDNRSDLWSLGITALEMAESQPPLCDLHPMRALFLIPRNSPPRLKSKKWSKKFHGFIDTVLVKDYHQRPYTENLLKHGFIKDQPTDRQVRIQLKDHIDRCKKRKQEKEREDYRYSGSDNDDDEPQLAGEHSSIVQAPGGDTLRRNFQQIQEGRLAAEQQQQHHLMAQAQAQAAAAHAAAQAQAQLQQQQQQAAAAAAAAAHAAQQAQQAAQQAQAQQPQANRQPKPPSRQQVEEPGPPARPPQRLIVVPDPPHANRPLPPTPKCGEPAGQTPQQQQRNSQNNFKPSLPPRRPEDHLDVLAAQLSELGVVFSQQPQPQTAAGGQGSQQQAQPEAPPRNNRQSSGLSSSGGSASGGGGSSKPAAALPQQSNNHLGQPVNPLDPLDSSDSDSEPDEPNDRARNDGTLLASDPPKPLPGLGPVSEDANTTTPLSHGSGGPPNRPLPPTPDDDDQAGDRTLIMKRNRGGGGGGGGGGGGGGSSGVGADGTGLGTPGTRTSSVLPDLLSQASPATPPRHDKSSSEEKQRSFLTFGFGAGGSGPSRRESHVNVNVTPTSHEAANDTPEIRKYKKRFNSEILCAALWGVNLLIGTENGLMLLDRSGQGKVYQLISRRRFQQMEVLEGQNILVTISGKKNRVRVYYLSWLKSKILRTDGLSDQVERRNGWINVGDLQGAVHFKIVKYERIKFLVIALKDSIEIYAWAPKPYHKFMAFKNFGELEHRPLLVDLTIEDQSRLKVIYGSAEGFHAVDLDSAEVYDIYLPKHTQGAIIPHCIVALPNSNGMQLLLCYDNEGVYVNTVGRVSKNIVLQWGEMPTSVAYIGTGQIMGWGNKAIEIRSVESGHLDGVFMHKKAQRLKFLCERNDKVFFSSAKGASSCQIYFMTLNKPGMANW

Target ORF information:

RefSeq Version	NM_206250.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster misshapen (msn), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206250.3

ORF Insert Sequence:

ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT 
GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA 
AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA 
AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG 
CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA 
ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC 
TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC 
ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT 
GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC 
TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC 
TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC 
TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC 
AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC 
TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC 
ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG 
CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA 
CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC 
AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG 
ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA 
CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA 
CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA 
AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC 
CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC 
GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG 
CCATCGTTAC CACCAAGGAG ACCTGAGGAT CATTTGGATG TGTTAGCAGC ACAATTAAGT 
GAATTGGGCG TGGTCTTCTC CCAACAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA 
TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG 
TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG 
CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC 
AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA 
TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC 
ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG 
CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA 
GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC 
ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG 
GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGAA GCAGCGCAGT 
TTCCTGACCT TTGGCTTCGG CGCCGGCGGC TCTGGTCCTT CGCGCCGGGA ATCGCACGTC 
AATGTCAATG TAACGCCCAC CTCCCACGAG GCGGCCAACG ATACGCCCGA GATCCGTAAG 
TATAAGAAGC GCTTCAATTC GGAAATCCTG TGCGCAGCGC TATGGGGCGT CAACCTGCTG 
ATTGGAACGG AGAATGGCTT AATGCTGCTA GATCGATCCG GTCAGGGCAA GGTCTACCAG 
CTCATCTCGC GACGCCGTTT CCAGCAAATG GAGGTGCTAG AGGGCCAGAA CATATTGGTC 
ACCATATCCG GCAAGAAGAA CCGAGTGCGC GTCTACTACT TGTCCTGGCT AAAGTCGAAG 
ATCTTGCGCA CCGACGGACT CTCCGATCAA GTGGAGCGTC GGAACGGTTG GATCAACGTG 
GGTGATCTGC AAGGTGCTGT ACATTTTAAG ATTGTCAAAT ACGAGAGGAT TAAGTTCCTA 
GTGATTGCAT TAAAAGACTC TATTGAAATT TATGCATGGG CTCCCAAACC ATATCACAAA 
TTTATGGCAT TTAAGAATTT TGGTGAACTG GAACATCGCC CGCTTTTGGT CGATCTCACC 
ATTGAGGATC AGTCGAGACT GAAGGTGATC TATGGCTCCG CCGAGGGTTT CCATGCGGTT 
GATTTAGATT CAGCTGAAGT ATACGATATC TATCTTCCCA AGCATACTCA GGGTGCAATC 
ATTCCGCATT GTATTGTGGC GCTTCCCAAC TCAAATGGCA TGCAACTGCT GCTGTGCTAC 
GACAATGAGG GCGTCTATGT GAACACTGTG GGCCGCGTTT CCAAGAACAT TGTACTGCAG 
TGGGGCGAGA TGCCCACCTC GGTGGCCTAC ATCGGCACTG GACAAATCAT GGGCTGGGGC 
AATAAAGCAA TAGAGATACG TTCCGTTGAG AGCGGCCATT TGGATGGTGT GTTCATGCAC 
AAAAAGGCGC AGCGCTTGAA ATTCCTTTGC GAGCGAAACG ACAAAGTATT CTTCAGCAGT 
GCCAAAGGTG CTTCATCGTG TCAAATCTAC TTTATGACGC TCAACAAGCC GGGCATGGCC 
AATTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msn ( NM_079940.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524679.3 Drosophila melanogaster misshapen (msn), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa20456
Clone ID Related Accession (Same CDS sequence)	NM_079940.5
Accession Version	NM_079940.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	misshapen, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079940.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT 
GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA 
AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA 
AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG 
CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA 
ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC 
TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC 
ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT 
GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC 
TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC 
TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC 
TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC 
AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC 
TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC 
ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG 
CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA 
CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC 
AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG 
ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA 
CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA 
CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA 
AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC 
CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC 
GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG 
CCATCGTTAC CACCAAGGAG ACCTGAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA 
TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG 
TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG 
CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC 
AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA 
TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC 
ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG 
CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA ACTAGAGCAG 
AACATAAACC GCCTGCAAAA GTCCGCATCC ACATCGCAAG CCAATGTGAC ACCGTCGCGT 
CGCGGCGACG AATCCAATTT GCTGCGGGAC TGGGACTTTG ATCGTTTCTT CCCCAAGAAC 
GCAAACGGTC CGAGGGGCAG TGGACGTGGT AGCCCCACAA CCACTGCCAG CTTGAGCCGA 
AGCTCCCAGC TGAGCACGCT GAAGGTGGAT ACGAAACTGC AAAGGGCCAG TGTGGCGGAG 
GCCATCACGA GACCAGTTCC CCGGGGCTAT CAGCCGCTAA AGGCCGAACC AAGTGCCAGC 
CAAAGTATTG CCAAAGAACA AGGATCAGCA TCAGGATCCG GATCGGGATC GGGAAGTGCT 
AGTGGTAGCG GAAGCGGTGG TAGCACCAAT TCCCCAGCGC ATAAGCGCCA GGATTCGGAC 
TCCCGATTGC CAACGAATTT TGAGCGCGGT TTTCGACGCG AGAACTCGGA CTTCTTCCCG 
CTGGCCAAGA GATACTCGGC TGTGTTCAGT GGTGCAAGTG CATCCGGATC AACAGCTGGA 
TCGCCGTCAG CACAGGCTCT TCAGAGGTCG AGCGCAGTGT ACCAAAGGAA CAGCATTTAT 
AGCAGCAGCA TCAGCAGTAA GAGCAAGGAG AATGACGTGC CTGGAGCGCC AGGAGCAGCT 
GCAGCAGGAA CTGCCACTGC CAGTGCCAAG CCAGGACCAG CTGCAGCCAC AACCACTGCC 
ACCAAGGGAG CTGCTCCAAA GACCTCCAAG TCCCTGGCCA ACTTTCATTT CCTGCGACCG 
CGTCGCGAAA AGACTGAATC CGTCATTGTG CTGCAGAATG CTGCCGTTCG CGCCCAAAGG 
CTGCAGCAAC TGCAACAGCA GCAGCAGCAG CAGCAACTGC AGCAGCAACA GCAGCAGCAG 
AATCGAGGAG GAGGCGGTGG CGGCGGCGGC GGCGGAGGAG GCGGTGGCAG CAGCGGCGTG 
GGCGCCGATG GCACTGGACT GGGAACTCCT GGGACAAGGA CCAGCAGCGT CCTGCCGGAT 
CTACTCAGCC AGGCATCGCC GGCAACGCCA CCACGCCATG ATAAATCATC CAGTGAGGAG 
TATCAAGCGG CCATTAGCTC ATCCGTGCAT TCCACGCCAT CGAAATCGTT TATCGCCAGC 
AGCGGAAGCG GTGGTCTCGG TTTGGGGGGC GGTACCGTTG TGGGCGGCGT GATTATCAGC 
AGCAATCACT CGCCCCAATC GACGATATCG CTCGCCTCTT CGTCCTCGAA TTCGCGCCAG 
AATTCGCCCA AGAATTCGAT CAGCAAGACG CGCTCCTCCA GCAGTATTAC TAATCTCTTG 
CACAAATCTG CTTCTTCCTC CTCCGCGAAC CTGCACCACC TGACGCCCTG CTCCTCGACG 
TCCTCGGCCT CGATCTCCAA TCCGCTGCCA CCGCACGCCT ATGCCCTGCA ACAGAAGCAG 
CGCAGTTTCC TGACCTTTGG CTTCGGCGCC GGCGGCTCTG GTCCTTCGCG CCGGGAATCG 
CACGTCAATG TCAATGTAAC GCCCACCTCC CACGAGGCGG CCAACGATAC GCCCGAGATC 
CGTAAGTATA AGAAGCGCTT CAATTCGGAA ATCCTGTGCG CAGCGCTATG GGGCGTCAAC 
CTGCTGATTG GAACGGAGAA TGGCTTAATG CTGCTAGATC GATCCGGTCA GGGCAAGGTC 
TACCAGCTCA TCTCGCGACG CCGTTTCCAG CAAATGGAGG TGCTAGAGGG CCAGAACATA 
TTGGTCACCA TATCCGGCAA GAAGAACCGA GTGCGCGTCT ACTACTTGTC CTGGCTAAAG 
TCGAAGATCT TGCGCACCGA CGGACTCTCC GATCAAGTGG AGCGTCGGAA CGGTTGGATC 
AACGTGGGTG ATCTGCAAGG TGCTGTACAT TTTAAGATTG TCAAATACGA GAGGATTAAG 
TTCCTAGTGA TTGCATTAAA AGACTCTATT GAAATTTATG CATGGGCTCC CAAACCATAT 
CACAAATTTA TGGCATTTAA GAATTTTGGT GAACTGGAAC ATCGCCCGCT TTTGGTCGAT 
CTCACCATTG AGGATCAGTC GAGACTGAAG GTGATCTATG GCTCCGCCGA GGGTTTCCAT 
GCGGTTGATT TAGATTCAGC TGAAGTATAC GATATCTATC TTCCCAAGCA TACTCAGGGT 
GCAATCATTC CGCATTGTAT TGTGGCGCTT CCCAACTCAA ATGGCATGCA ACTGCTGCTG 
TGCTACGACA ATGAGGGCGT CTATGTGAAC ACTGTGGGCC GCGTTTCCAA GAACATTGTA 
CTGCAGTGGG GCGAGATGCC CACCTCGGTG GCCTACATCG GCACTGGACA AATCATGGGC 
TGGGGCAATA AAGCAATAGA GATACGTTCC GTTGAGAGCG GCCATTTGGA TGGTGTGTTC 
ATGCACAAAA AGGCGCAGCG CTTGAAATTC CTTTGCGAGC GAAACGACAA AGTATTCTTC 
AGCAGTGCCA AAGGTGCTTC ATCGTGTCAA ATCTACTTTA TGACGCTCAA CAAGCCGGGC 
ATGGCCAATT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524679.3
CDS	195..4709
Translation	MAHQQQQQLAPSVNCSLDDIDLTALKDPAGIFELIEVVGNGTYGQVYKGRHTKTGQLAAIKVMDVTEDEEEEIKLEINVLKKYSNHRNIATYYGAFIKKSPPGKDDQLWLVMEYCGAGSVTDLVKSTKGQSLKEEWIAYICREILRGLSYLHSNKVIHRDIKGQNVLLTDNAEVKLVDFGVSAQLDRTIGRRNTFIGTPYWMAPEVIACDENPDATYDNRSDLWSLGITALEMAESQPPLCDLHPMRALFLIPRNSPPRLKSKKWSKKFHGFIDTVLVKDYHQRPYTENLLKHGFIKDQPTDRQVRIQLKDHIDRCKKRKQEKEREDYRYSGSDNDDDEPQLAGEHSSIVQAPGGDTLRRNFQQIQEGRLAAEQQQQHHLMAQAQAQAAAAHAAAQAQAQLQQQQQQAAAAAAAAAHAAQQAQQAAQQAQAQQPQANRQPKPPSRQQVEEPGPPARPPQRLIVVPDPPHANRPLPPTPKCGEPAGQTPQQQQRNSQNNFKPSLPPRRPEPQPQTAAGGQGSQQQAQPEAPPRNNRQSSGLSSSGGSASGGGGSSKPAAALPQQSNNHLGQPVNPLDPLDSSDSDSEPDEPNDRARNDGTLLASDPPKPLPGLGPVSEDANTTTPLSHGSGGPPNRPLPPTPDDDDQAGDRTLIMKRKLEQNINRLQKSASTSQANVTPSRRGDESNLLRDWDFDRFFPKNANGPRGSGRGSPTTTASLSRSSQLSTLKVDTKLQRASVAEAITRPVPRGYQPLKAEPSASQSIAKEQGSASGSGSGSGSASGSGSGGSTNSPAHKRQDSDSRLPTNFERGFRRENSDFFPLAKRYSAVFSGASASGSTAGSPSAQALQRSSAVYQRNSIYSSSISSKSKENDVPGAPGAAAAGTATASAKPGPAAATTTATKGAAPKTSKSLANFHFLRPRREKTESVIVLQNAAVRAQRLQQLQQQQQQQQLQQQQQQQNRGGGGGGGGGGGGGGSSGVGADGTGLGTPGTRTSSVLPDLLSQASPATPPRHDKSSSEEYQAAISSSVHSTPSKSFIASSGSGGLGLGGGTVVGGVIISSNHSPQSTISLASSSSNSRQNSPKNSISKTRSSSSITNLLHKSASSSSANLHHLTPCSSTSSASISNPLPPHAYALQQKQRSFLTFGFGAGGSGPSRRESHVNVNVTPTSHEAANDTPEIRKYKKRFNSEILCAALWGVNLLIGTENGLMLLDRSGQGKVYQLISRRRFQQMEVLEGQNILVTISGKKNRVRVYYLSWLKSKILRTDGLSDQVERRNGWINVGDLQGAVHFKIVKYERIKFLVIALKDSIEIYAWAPKPYHKFMAFKNFGELEHRPLLVDLTIEDQSRLKVIYGSAEGFHAVDLDSAEVYDIYLPKHTQGAIIPHCIVALPNSNGMQLLLCYDNEGVYVNTVGRVSKNIVLQWGEMPTSVAYIGTGQIMGWGNKAIEIRSVESGHLDGVFMHKKAQRLKFLCERNDKVFFSSAKGASSCQIYFMTLNKPGMANW

Target ORF information:

RefSeq Version	NM_079940.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster misshapen (msn), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079940.5

ORF Insert Sequence:

ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT 
GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA 
AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA 
AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG 
CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA 
ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC 
TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC 
ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT 
GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC 
TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC 
TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC 
TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC 
AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC 
TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC 
ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG 
CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA 
CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC 
AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG 
ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA 
CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA 
CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA 
AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC 
CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC 
GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG 
CCATCGTTAC CACCAAGGAG ACCTGAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA 
TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG 
TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG 
CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC 
AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA 
TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC 
ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG 
CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA ACTAGAGCAG 
AACATAAACC GCCTGCAAAA GTCCGCATCC ACATCGCAAG CCAATGTGAC ACCGTCGCGT 
CGCGGCGACG AATCCAATTT GCTGCGGGAC TGGGACTTTG ATCGTTTCTT CCCCAAGAAC 
GCAAACGGTC CGAGGGGCAG TGGACGTGGT AGCCCCACAA CCACTGCCAG CTTGAGCCGA 
AGCTCCCAGC TGAGCACGCT GAAGGTGGAT ACGAAACTGC AAAGGGCCAG TGTGGCGGAG 
GCCATCACGA GACCAGTTCC CCGGGGCTAT CAGCCGCTAA AGGCCGAACC AAGTGCCAGC 
CAAAGTATTG CCAAAGAACA AGGATCAGCA TCAGGATCCG GATCGGGATC GGGAAGTGCT 
AGTGGTAGCG GAAGCGGTGG TAGCACCAAT TCCCCAGCGC ATAAGCGCCA GGATTCGGAC 
TCCCGATTGC CAACGAATTT TGAGCGCGGT TTTCGACGCG AGAACTCGGA CTTCTTCCCG 
CTGGCCAAGA GATACTCGGC TGTGTTCAGT GGTGCAAGTG CATCCGGATC AACAGCTGGA 
TCGCCGTCAG CACAGGCTCT TCAGAGGTCG AGCGCAGTGT ACCAAAGGAA CAGCATTTAT 
AGCAGCAGCA TCAGCAGTAA GAGCAAGGAG AATGACGTGC CTGGAGCGCC AGGAGCAGCT 
GCAGCAGGAA CTGCCACTGC CAGTGCCAAG CCAGGACCAG CTGCAGCCAC AACCACTGCC 
ACCAAGGGAG CTGCTCCAAA GACCTCCAAG TCCCTGGCCA ACTTTCATTT CCTGCGACCG 
CGTCGCGAAA AGACTGAATC CGTCATTGTG CTGCAGAATG CTGCCGTTCG CGCCCAAAGG 
CTGCAGCAAC TGCAACAGCA GCAGCAGCAG CAGCAACTGC AGCAGCAACA GCAGCAGCAG 
AATCGAGGAG GAGGCGGTGG CGGCGGCGGC GGCGGAGGAG GCGGTGGCAG CAGCGGCGTG 
GGCGCCGATG GCACTGGACT GGGAACTCCT GGGACAAGGA CCAGCAGCGT CCTGCCGGAT 
CTACTCAGCC AGGCATCGCC GGCAACGCCA CCACGCCATG ATAAATCATC CAGTGAGGAG 
TATCAAGCGG CCATTAGCTC ATCCGTGCAT TCCACGCCAT CGAAATCGTT TATCGCCAGC 
AGCGGAAGCG GTGGTCTCGG TTTGGGGGGC GGTACCGTTG TGGGCGGCGT GATTATCAGC 
AGCAATCACT CGCCCCAATC GACGATATCG CTCGCCTCTT CGTCCTCGAA TTCGCGCCAG 
AATTCGCCCA AGAATTCGAT CAGCAAGACG CGCTCCTCCA GCAGTATTAC TAATCTCTTG 
CACAAATCTG CTTCTTCCTC CTCCGCGAAC CTGCACCACC TGACGCCCTG CTCCTCGACG 
TCCTCGGCCT CGATCTCCAA TCCGCTGCCA CCGCACGCCT ATGCCCTGCA ACAGAAGCAG 
CGCAGTTTCC TGACCTTTGG CTTCGGCGCC GGCGGCTCTG GTCCTTCGCG CCGGGAATCG 
CACGTCAATG TCAATGTAAC GCCCACCTCC CACGAGGCGG CCAACGATAC GCCCGAGATC 
CGTAAGTATA AGAAGCGCTT CAATTCGGAA ATCCTGTGCG CAGCGCTATG GGGCGTCAAC 
CTGCTGATTG GAACGGAGAA TGGCTTAATG CTGCTAGATC GATCCGGTCA GGGCAAGGTC 
TACCAGCTCA TCTCGCGACG CCGTTTCCAG CAAATGGAGG TGCTAGAGGG CCAGAACATA 
TTGGTCACCA TATCCGGCAA GAAGAACCGA GTGCGCGTCT ACTACTTGTC CTGGCTAAAG 
TCGAAGATCT TGCGCACCGA CGGACTCTCC GATCAAGTGG AGCGTCGGAA CGGTTGGATC 
AACGTGGGTG ATCTGCAAGG TGCTGTACAT TTTAAGATTG TCAAATACGA GAGGATTAAG 
TTCCTAGTGA TTGCATTAAA AGACTCTATT GAAATTTATG CATGGGCTCC CAAACCATAT 
CACAAATTTA TGGCATTTAA GAATTTTGGT GAACTGGAAC ATCGCCCGCT TTTGGTCGAT 
CTCACCATTG AGGATCAGTC GAGACTGAAG GTGATCTATG GCTCCGCCGA GGGTTTCCAT 
GCGGTTGATT TAGATTCAGC TGAAGTATAC GATATCTATC TTCCCAAGCA TACTCAGGGT 
GCAATCATTC CGCATTGTAT TGTGGCGCTT CCCAACTCAA ATGGCATGCA ACTGCTGCTG 
TGCTACGACA ATGAGGGCGT CTATGTGAAC ACTGTGGGCC GCGTTTCCAA GAACATTGTA 
CTGCAGTGGG GCGAGATGCC CACCTCGGTG GCCTACATCG GCACTGGACA AATCATGGGC 
TGGGGCAATA AAGCAATAGA GATACGTTCC GTTGAGAGCG GCCATTTGGA TGGTGTGTTC 
ATGCACAAAA AGGCGCAGCG CTTGAAATTC CTTTGCGAGC GAAACGACAA AGTATTCTTC 
AGCAGTGCCA AAGGTGCTTC ATCGTGTCAA ATCTACTTTA TGACGCTCAA CAAGCCGGGC 
ATGGCCAATT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msn ( NM_206249.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995971.2 Drosophila melanogaster misshapen (msn), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa20457
Clone ID Related Accession (Same CDS sequence)	NM_206249.3
Accession Version	NM_206249.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	misshapen, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206249.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT 
GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA 
AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA 
AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG 
CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA 
ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC 
TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC 
ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT 
GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC 
TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC 
TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC 
TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC 
AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC 
TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC 
ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG 
CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA 
CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC 
AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG 
ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA 
CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA 
CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA 
AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC 
CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC 
GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG 
CCATCGTTAC CACCAAGGAG ACCTGAGGAT CATTTGGATG TGTTAGCAGC ACAATTAAGT 
GAATTGGGCG TGGTCTTCTC CCAACAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA 
TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG 
TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG 
CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC 
AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA 
TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC 
ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG 
CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA 
GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC 
ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG 
GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGCA GCGCAGTTTC 
CTGACCTTTG GCTTCGGCGC CGGCGGCTCT GGTCCTTCGC GCCGGGAATC GCACGTCAAT 
GTCAATGTAA CGCCCACCTC CCACGAGGCG GCCAACGATA CGCCCGAGAT CCGTAAGTAT 
AAGAAGCGCT TCAATTCGGA AATCCTGTGC GCAGCGCTAT GGGGCGTCAA CCTGCTGATT 
GGAACGGAGA ATGGCTTAAT GCTGCTAGAT CGATCCGGTC AGGGCAAGGT CTACCAGCTC 
ATCTCGCGAC GCCGTTTCCA GCAAATGGAG GTGCTAGAGG GCCAGAACAT ATTGGTCACC 
ATATCCGGCA AGAAGAACCG AGTGCGCGTC TACTACTTGT CCTGGCTAAA GTCGAAGATC 
TTGCGCACCG ACGGACTCTC CGATCAAGTG GAGCGTCGGA ACGGTTGGAT CAACGTGGGT 
GATCTGCAAG GTGCTGTACA TTTTAAGATT GTCAAATACG AGAGGATTAA GTTCCTAGTG 
ATTGCATTAA AAGACTCTAT TGAAATTTAT GCATGGGCTC CCAAACCATA TCACAAATTT 
ATGGCATTTA AGAATTTTGG TGAACTGGAA CATCGCCCGC TTTTGGTCGA TCTCACCATT 
GAGGATCAGT CGAGACTGAA GGTGATCTAT GGCTCCGCCG AGGGTTTCCA TGCGGTTGAT 
TTAGATTCAG CTGAAGTATA CGATATCTAT CTTCCCAAGC ATACTCAGGG TGCAATCATT 
CCGCATTGTA TTGTGGCGCT TCCCAACTCA AATGGCATGC AACTGCTGCT GTGCTACGAC 
AATGAGGGCG TCTATGTGAA CACTGTGGGC CGCGTTTCCA AGAACATTGT ACTGCAGTGG 
GGCGAGATGC CCACCTCGGT GGCCTACATC GGCACTGGAC AAATCATGGG CTGGGGCAAT 
AAAGCAATAG AGATACGTTC CGTTGAGAGC GGCCATTTGG ATGGTGTGTT CATGCACAAA 
AAGGCGCAGC GCTTGAAATT CCTTTGCGAG CGAAACGACA AAGTATTCTT CAGCAGTGCC 
AAAGGTGCTT CATCGTGTCA AATCTACTTT ATGACGCTCA ACAAGCCGGG CATGGCCAAT 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995971.2
CDS	195..3500
Translation	MAHQQQQQLAPSVNCSLDDIDLTALKDPAGIFELIEVVGNGTYGQVYKGRHTKTGQLAAIKVMDVTEDEEEEIKLEINVLKKYSNHRNIATYYGAFIKKSPPGKDDQLWLVMEYCGAGSVTDLVKSTKGQSLKEEWIAYICREILRGLSYLHSNKVIHRDIKGQNVLLTDNAEVKLVDFGVSAQLDRTIGRRNTFIGTPYWMAPEVIACDENPDATYDNRSDLWSLGITALEMAESQPPLCDLHPMRALFLIPRNSPPRLKSKKWSKKFHGFIDTVLVKDYHQRPYTENLLKHGFIKDQPTDRQVRIQLKDHIDRCKKRKQEKEREDYRYSGSDNDDDEPQLAGEHSSIVQAPGGDTLRRNFQQIQEGRLAAEQQQQHHLMAQAQAQAAAAHAAAQAQAQLQQQQQQAAAAAAAAAHAAQQAQQAAQQAQAQQPQANRQPKPPSRQQVEEPGPPARPPQRLIVVPDPPHANRPLPPTPKCGEPAGQTPQQQQRNSQNNFKPSLPPRRPEDHLDVLAAQLSELGVVFSQQPQPQTAAGGQGSQQQAQPEAPPRNNRQSSGLSSSGGSASGGGGSSKPAAALPQQSNNHLGQPVNPLDPLDSSDSDSEPDEPNDRARNDGTLLASDPPKPLPGLGPVSEDANTTTPLSHGSGGPPNRPLPPTPDDDDQAGDRTLIMKRNRGGGGGGGGGGGGGGSSGVGADGTGLGTPGTRTSSVLPDLLSQASPATPPRHDKSSSEEQRSFLTFGFGAGGSGPSRRESHVNVNVTPTSHEAANDTPEIRKYKKRFNSEILCAALWGVNLLIGTENGLMLLDRSGQGKVYQLISRRRFQQMEVLEGQNILVTISGKKNRVRVYYLSWLKSKILRTDGLSDQVERRNGWINVGDLQGAVHFKIVKYERIKFLVIALKDSIEIYAWAPKPYHKFMAFKNFGELEHRPLLVDLTIEDQSRLKVIYGSAEGFHAVDLDSAEVYDIYLPKHTQGAIIPHCIVALPNSNGMQLLLCYDNEGVYVNTVGRVSKNIVLQWGEMPTSVAYIGTGQIMGWGNKAIEIRSVESGHLDGVFMHKKAQRLKFLCERNDKVFFSSAKGASSCQIYFMTLNKPGMANW

Target ORF information:

RefSeq Version	NM_206249.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster misshapen (msn), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206249.3

ORF Insert Sequence:

ATGGCGCACC AGCAGCAACA ACAACTGGCT CCATCGGTCA ACTGTTCACT GGACGATATC 
GATCTGACGG CCCTAAAAGA TCCAGCTGGC ATCTTTGAGC TCATCGAAGT TGTGGGCAAT 
GGCACCTACG GTCAGGTCTA CAAGGGCCGT CACACGAAGA CTGGTCAATT GGCTGCCATA 
AAGGTGATGG ACGTCACCGA GGACGAAGAG GAGGAGATCA AGCTGGAGAT CAATGTGCTA 
AAGAAATACT CGAATCACCG CAACATCGCC ACCTACTACG GAGCCTTCAT CAAGAAGTCG 
CCACCTGGGA AGGACGATCA GCTCTGGCTG GTGATGGAGT ACTGTGGTGC TGGATCGGTA 
ACGGATCTGG TCAAGTCCAC CAAGGGTCAG AGCCTGAAGG AGGAGTGGAT CGCCTACATC 
TGCCGCGAGA TTCTGCGGGG CCTGAGCTAC CTGCACTCGA ACAAAGTCAT CCATCGCGAC 
ATCAAGGGGC AGAATGTGCT CCTCACCGAC AACGCCGAGG TGAAGCTGGT GGACTTTGGT 
GTCTCCGCCC AGCTGGATCG CACGATAGGA CGGCGCAACA CATTCATCGG TACTCCCTAC 
TGGATGGCCC CCGAGGTCAT TGCCTGCGAC GAGAATCCCG ACGCCACGTA CGACAATCGC 
TCCGATCTGT GGTCGCTGGG CATCACCGCT CTGGAGATGG CTGAGTCACA GCCCCCGCTC 
TGCGATCTGC ATCCGATGCG CGCCCTCTTC CTGATTCCGC GCAACTCGCC ACCCAGGCTC 
AAATCGAAGA AGTGGTCCAA GAAGTTTCAC GGCTTTATTG ACACGGTGCT AGTTAAGGAC 
TATCACCAGC GGCCTTACAC CGAGAACCTG CTGAAGCACG GCTTCATCAA GGACCAGCCC 
ACAGATCGCC AGGTGCGCAT CCAGCTGAAG GACCACATCG ATCGCTGCAA GAAGCGCAAG 
CAGGAGAAGG AGCGCGAGGA CTATCGCTAC TCGGGCTCCG ACAACGACGA CGACGAACCA 
CAGCTGGCCG GTGAGCACAG CTCCATTGTC CAGGCACCAG GCGGCGATAC GTTGCGCCGC 
AACTTCCAGC AGATCCAGGA AGGTCGCCTG GCCGCCGAGC AGCAGCAGCA GCATCACCTG 
ATGGCCCAGG CGCAGGCTCA GGCGGCTGCC GCTCATGCCG CTGCCCAGGC CCAGGCGCAA 
CTCCAGCAGC AGCAACAGCA GGCTGCGGCG GCGGCAGCAG CGGCGGCCCA TGCAGCACAA 
CAGGCTCAGC AGGCCGCACA GCAGGCGCAG GCCCAGCAGC CACAGGCCAA TCGGCAGCCA 
AAACCGCCAT CGCGCCAGCA GGTTGAGGAG CCGGGTCCGC CAGCACGTCC GCCACAGCGC 
CTGATCGTGG TGCCGGATCC GCCGCACGCC AATCGGCCAT TGCCACCGAC GCCCAAGTGC 
GGCGAGCCGG CGGGACAGAC GCCGCAGCAA CAGCAGCGCA ATTCGCAGAA TAACTTCAAG 
CCATCGTTAC CACCAAGGAG ACCTGAGGAT CATTTGGATG TGTTAGCAGC ACAATTAAGT 
GAATTGGGCG TGGTCTTCTC CCAACAGCCA CAGCCGCAGA CGGCCGCCGG TGGTCAGGGA 
TCCCAGCAGC AGGCGCAGCC GGAGGCGCCG CCTCGCAACA ATCGCCAGTC GAGCGGTCTG 
TCCTCCTCCG GCGGATCGGC ATCCGGAGGC GGCGGTTCGT CGAAGCCCGC AGCCGCTCTG 
CCGCAGCAGT CGAACAATCA TTTGGGCCAG CCGGTGAATC CGCTGGATCC CTTGGACAGC 
AGCGATTCGG ACAGCGAGCC GGATGAGCCC AACGATCGGG CTAGGAACGA TGGAACTCTA 
TTGGCCAGTG ATCCGCCCAA GCCACTGCCC GGCTTGGGAC CCGTCTCCGA GGACGCCAAC 
ACGACAACGC CCTTGAGTCA CGGCAGCGGT GGACCGCCCA ACCGCCCACT GCCGCCCACG 
CCCGACGACG ACGATCAGGC TGGCGATCGG ACTTTGATCA TGAAGCGCAA TCGAGGAGGA 
GGCGGTGGCG GCGGCGGCGG CGGAGGAGGC GGTGGCAGCA GCGGCGTGGG CGCCGATGGC 
ACTGGACTGG GAACTCCTGG GACAAGGACC AGCAGCGTCC TGCCGGATCT ACTCAGCCAG 
GCATCGCCGG CAACGCCACC ACGCCATGAT AAATCATCCA GTGAGGAGCA GCGCAGTTTC 
CTGACCTTTG GCTTCGGCGC CGGCGGCTCT GGTCCTTCGC GCCGGGAATC GCACGTCAAT 
GTCAATGTAA CGCCCACCTC CCACGAGGCG GCCAACGATA CGCCCGAGAT CCGTAAGTAT 
AAGAAGCGCT TCAATTCGGA AATCCTGTGC GCAGCGCTAT GGGGCGTCAA CCTGCTGATT 
GGAACGGAGA ATGGCTTAAT GCTGCTAGAT CGATCCGGTC AGGGCAAGGT CTACCAGCTC 
ATCTCGCGAC GCCGTTTCCA GCAAATGGAG GTGCTAGAGG GCCAGAACAT ATTGGTCACC 
ATATCCGGCA AGAAGAACCG AGTGCGCGTC TACTACTTGT CCTGGCTAAA GTCGAAGATC 
TTGCGCACCG ACGGACTCTC CGATCAAGTG GAGCGTCGGA ACGGTTGGAT CAACGTGGGT 
GATCTGCAAG GTGCTGTACA TTTTAAGATT GTCAAATACG AGAGGATTAA GTTCCTAGTG 
ATTGCATTAA AAGACTCTAT TGAAATTTAT GCATGGGCTC CCAAACCATA TCACAAATTT 
ATGGCATTTA AGAATTTTGG TGAACTGGAA CATCGCCCGC TTTTGGTCGA TCTCACCATT 
GAGGATCAGT CGAGACTGAA GGTGATCTAT GGCTCCGCCG AGGGTTTCCA TGCGGTTGAT 
TTAGATTCAG CTGAAGTATA CGATATCTAT CTTCCCAAGC ATACTCAGGG TGCAATCATT 
CCGCATTGTA TTGTGGCGCT TCCCAACTCA AATGGCATGC AACTGCTGCT GTGCTACGAC 
AATGAGGGCG TCTATGTGAA CACTGTGGGC CGCGTTTCCA AGAACATTGT ACTGCAGTGG 
GGCGAGATGC CCACCTCGGT GGCCTACATC GGCACTGGAC AAATCATGGG CTGGGGCAAT 
AAAGCAATAG AGATACGTTC CGTTGAGAGC GGCCATTTGG ATGGTGTGTT CATGCACAAA 
AAGGCGCAGC GCTTGAAATT CCTTTGCGAG CGAAACGACA AAGTATTCTT CAGCAGTGCC 
AAAGGTGCTT CATCGTGTCA AATCTACTTT ATGACGCTCA ACAAGCCGGG CATGGCCAAT 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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msn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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