Ptp99A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptp99A
Entrez Gene ID	43469
Full Name	Protein tyrosine phosphatase 99A
Synonyms	CG11515,CG11516,CG11517,CG2005,CT6383,DPTP99A,DPTP[[99A]],Dmel\CG11516,PTP99A,R-PTP 99A,dptp99A,ptp99A
General protein information	Preferred Names Protein tyrosine phosphatase 99A Names CG11516 gene product from transcript CG11516-RF CG11516-PA CG11516-PB CG11516-PC CG11516-PF CG11516-PG Ptp99A-PA Ptp99A-PB Ptp99A-PC Ptp99A-PF Ptp99A-PG protein tyrosine phosphatase 99A
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99A9-99B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_143434.5	NP_651691.4	protein tyrosine phosphatase 99A, isoform F
NM_057468.3	NP_476816.1	protein tyrosine phosphatase 99A, isoform B
NM_001202380.2	NP_001189309.1	protein tyrosine phosphatase 99A, isoform G
NM_170409.3	NP_733288.1	protein tyrosine phosphatase 99A, isoform A
NM_206579.3	NP_996302.1	protein tyrosine phosphatase 99A, isoform C

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002832	XP_003436255.1
Drosophila melanogaster (fruit fly)	Ptp99A	NP_651691.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

During embryonic development of the mushroom body, Ptp99A is expressed within an expanded core region including somewhat older axons.
Title: Receptor tyrosine phosphatases regulate birth order-dependent axonal fasciculation and midline repulsion during development of the Drosophila mushroom body.

The following Ptp99A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptp99A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06490	NM_143434.5 Latest version!	Drosophila melanogaster protein tyrosine phosphatase 99A, transcript variant F (Ptp99A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa06491	NM_206579.3 Latest version!	Drosophila melanogaster protein tyrosine phosphatase 99A, transcript variant C (Ptp99A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa06492	NM_170409.3 Latest version!	Drosophila melanogaster protein tyrosine phosphatase 99A, transcript variant A (Ptp99A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa06493	NM_057468.3 Latest version!	Drosophila melanogaster protein tyrosine phosphatase 99A, transcript variant B (Ptp99A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa08950	NM_001202380.2 Latest version!	Drosophila melanogaster protein tyrosine phosphatase 99A, transcript variant G (Ptp99A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptp99A ( NM_143434.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651691.4 Drosophila melanogaster protein tyrosine phosphatase 99A, transcript variant F (Ptp99A), mRNA.

Price & Availability↑

CloneID	OFa06490
Clone ID Related Accession (Same CDS sequence)	NM_143434.5
Accession Version	NM_143434.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein tyrosine phosphatase 99A, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143434.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCAGC GCATATCGGC CACATACGGA AATCATCGAG GGCTCATCAT TCCTGGCTTT 
GTGCTGCTCC TGATTTTGGC CACCTTGCCT GCCACGCAGG CAGGACGAAT CTTCGACGTC 
ACAAATCTGG ACTACGTAAA GGTGAATGCC GAGGCGGGCA AGAACGTGAC CATTCCGTGT 
CCGGGGGTCA ACGAGCACTC GCTGGTGGAC ACGCTCGTGT GGAAGACGGC GTCCACGACA 
ATCGCACAGT TTGCCAACCG GATACCCCTG CTCCACAGTC CGAGGATTCA ACTTCTTTCG 
GACAACTTCG GCCTGCAGTT TTCCCCTTCA TTAGCGAGTG ATACCTCGGA ATACATATGC 
CTGGTGAACG ATCGCCATAT ACCAGAAGCC ATTGTCGATT TGCTGGTCCA GGATGTGCCC 
GATCCGCCGG AGCGACCATT GTTGATAAGT TTCACATCAC GCTCGGTGAA TTTATCCTGG 
GCGCACTCAC AGCATCCGCG GAATGCGCCC GTCACGCATT TTATTATCGA AACGCGGGAG 
GGCGAGGATG GCATTTGGGA GCAGCTGGCC AGGATCTATA CGAAGACAAA TGCGACGTCA 
TATCAGGTGA CCGGGCTGAC GCCCTTCACC GTCTACAGCT TTCGTCTGCT GGCGGTCAAC 
AAGCTGGGTA TCAGTCCGCC GTCCAAGGAG TCCTACTACA TAGTCACGCT GCGTGAGGCT 
CCCACGGGCA AGCCCATACC GACGACGGCC CACAACACGT CCTCCACCTC GGTGTACATC 
TCGTGGAAGG CACCGCCGCC GGATACCATA CTGGGCGAAT TCCTTGGCTA CCGCATCACA 
TATCGACCTC GCGATCGGGA CCCCAACGAT ACCAAGGAAA TCTATATACG CGACAACACC 
GTGGAGAGCC ATGAAATTCA AAACCTACAG ACGTACACGC AGTACAAGGT GACAGTGCAG 
GTGTTCAATC CCGAGGGTCT GGGACCGGAG ACCACCATCC TGGTGATGAC CGACGAAGGA 
GTGCCAAGCA AACCGCAGAA TCTAACCATC TTGGATGTTT CGGCCAACAG CATCACGATG 
TCCTGGCATC CGCCAAAGAA TCAAAACGGA GCCATCGCCG GTTACCATGT GTTCCACATA 
CACGACAACC AAACTGGCGT GGAGATCGTC AAGAACTCTC GCAATTCGGT GGAAACCCTC 
ATTCACTTCG AACTGCAGAA CTTACGACCC TACACCGACT ACCGTGTGAT AGTCAAGGCA 
TTCACCACCA AGAACGAGGG CGAACCCTCC GATCAGATTG CCCAGCGTAC GGATGTCGGA 
GGACCCAGTG CCCCGGCGAT TGTGAACCTC ACTTGTCACT CCCAGGAGTC CATCACGATT 
CGATGGCGAA GACCCTATGA GTTCTACAAC ACCATCGACT TTTACATCAT CAAGACGCGT 
TTGGCTGGCC AGGATACGCA TCGGGATATC AGGATTAATG CCTCGGCCAA GGAACTGGAA 
ACTGCGATGA TTCTTCAAAA CCTAACAACC AACTCGTATT ACGAGGTCAA AGTGGCAGCG 
GCAACGTTCA GTGTCATAAA TCCAAAGAAA ATTGTGCTGG GCAAATTCTC GGAGTCAAGG 
ATTATACAAC TGCAACCGAA CTGCGAGAAA TTGCAACCGC TGCTGCGGCA GTCGCATAAT 
GACTACAACC TGGCCGTTCT GGTGGGCATC ATCTTCAGCT GCTTCGGCAT CATCCTGATC 
ATCATGGCCT TCTTCCTGTG GAGCAGAAAG TGCTTCCATG CGGCCTACTA CTATCTGGAC 
GACCCGCCAC ACCATCCAAA TGCCCCGCAG GTGGACTGGG AAGTGCCCGT TAAGATCGGC 
GACGAGATCC GAGCGGCTGT GCCCGTCAAC GAGTTCGCCA AGCACGTGGC TTCCCTGCAC 
GCGGACGGGG ACATTGGATT CAGCCGGGAG TACGAGGCCA TCCAGAACGA GTGCATCAGC 
GATGATCTGC CGTGTGAACA CTCGCAGCAT CCGGAGAACA AACGCAAGAA TCGCTATCTC 
AACATCACGG CCTATGACCA CAGTCGGGTG CATCTGCATC CCACACCGGG ACAGAAGAAG 
AATCTGGACT ACATCAATGC CAACTTCATC GATGGCTACC AGAAGGGACA TGCCTTCATC 
GGCACCCAGG GTCCATTGCC CGACACCTTC GATTGCTTCT GGCGCATGAT CTGGGAACAG 
AGGGTGGCCA TCATCGTGAT GATCACCAAT CTTGTGGAGC GCGGGCGGCG CAAGTGCGAC 
ATGTACTGGC CGAAGGATGG GGTGGAGACC TATGGCGTAA TCCAGGTCAA GCTCATCGAG 
GAGGAGGTCA TGTCCACGTA CACTGTGCGC ACCCTGCAGA TCAAGCACTT GAAGCTCAAG 
AAGAAGAAGC AGTGCAACAC CGAAAAGCTG GTCTATCAAT ACCACTACAC CAACTGGCCC 
GACCACGGAA CACCCGATCA TCCCCTACCC GTGCTTAACT TTGTCAAGAA ATCCTCGGCC 
GCCAATCCCG CAGAGGCTGG TCCCATAGTC GTGCACTGCA GCGCTGGCGT CGGTCGCACT 
GGCACATACA TCGTCCTGGA CGCCATGCTC AAGCAGATCC AGCAGAAGAA CATCGTCAAC 
GTCTTTGGCT TTCTGCGTCA CATTCGTGCT CAGAGGAACT TCCTGGTGCA GACCGAGGAG 
CAGTACATAT TCCTGCACGA CGCTCTGGTG GAGGCCATCG CCTCGGGGGA GACCAATCTG 
ATGGCCGAGC AGGTGGAGGA GCTGAAGAAC TGCACTCCCT ACCTGGAGCA GCAGTACAAG 
AACATCATCC AGTTCCAACC GAAAGACATA CACATTGCAT CCGCCATGAA GCAGGTGAAC 
TCGATCAAGA ACCGCGGCGC CATCTTCCCC ATCGAGGGCA GTCGGGTGCA CTTGACGCCC 
AAGCCGGGCG AGGATGGCAG CGATTATATC AACGCCTCGT GGCTGCACGG CTTCCGGCGA 
CTGCGGGACT TCATAGTCAC CCAACATCCC ATGGCGCACA CGATAAAGGA CTTCTGGCAG 
ATGGTCTGGG ACCACAATGC GCAGACCGTC GTGCTGCTCT CATCACTGGA TGATATAAAC 
TTTGCACAGT TTTGGCCAGA TGAGGCCACG CCCATCGAGA GCGATCACTA TCGCGTCAAG 
TTTCTGAACA AGACCAACAA GAGCGACTAT GTGAGCCGCG ACTTTGTCAT CCAGTCGATA 
CAGGACGACT ACGAGCTGAC GGTCAAGATG CTGCACTGTC CCAGTTGGCC AGAGATGTCC 
AATCCCAACA GCATCTACGA CTTCATCGTC GATGTGCACG AGCGCTGCAA TGACTATCGC 
AATGGACCCA TCGTCATTGT GGATAGATAT GGTGGCGCCC AGGCGTGCAC CTTCTGTGCC 
ATCTCCTCGC TGGCCATCGA GATGGAGTAC TGCAGCACGG CGAATGTGTA CCAGTACGCG 
AAGCTGTACC ACAACAAGCG ACCCGGGGTG TGGACATCCA GCGAGGACAT CCGCGTCATC 
TACAACATAC TTTCATTTTT GCCTGGCAAT TTGAACCTGC TGAAGCGCAC GGCGCTTCGG 
ACTGAATTCG AGGATGTGAC AACGGCCACG CCGGATCTCT ATAGCAAAAT ATGCAGCAAT 
GTCGCTCCTC TATCCTCCAG TTCATCCACC ACACCACCAC CACCAAGTAC TCCAACACCA 
CAGCCCCCAC AAACCATCCA ACTGTCATCG CATTCGCCAT CCGACCTGTC CCACCAGATC 
TCATCCACAG TCGCCAATGC AGGATCACCA GCAACTGCAA GTGCAGGCGC AACACCAACG 
ACTCCAATGA CACCGACAGT CCCACCCACA ATACCTACAA TACCATCACT CGCATCCCAG 
AACAGTCTAA CCCTTACTAA TGCCAATTTC CATACTGTTA CTAATAACGC TGCTGATCTG 
ATGGAACACC AACAACAACA AATGCTGGCC CTGATGCAAC AACAAACGCA ACTGCAACAA 
CAATACAACA CGCACCCGCA ACAACATCAC AACAACGTCG GTGACCTGCT GATGAACAAT 
GCGGACAACT CACCAACCGC TTCACCAACG ATCACCAACA ACAATCATAT AACCAACAAC 
AACGTCACAA GCGCAGCAGC GACAGATGCT CAAAACCTAG ATATTGTAGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651691.4
CDS	701..4894
Translation	MTQRISATYGNHRGLIIPGFVLLLILATLPATQAGRIFDVTNLDYVKVNAEAGKNVTIPCPGVNEHSLVDTLVWKTASTTIAQFANRIPLLHSPRIQLLSDNFGLQFSPSLASDTSEYICLVNDRHIPEAIVDLLVQDVPDPPERPLLISFTSRSVNLSWAHSQHPRNAPVTHFIIETREGEDGIWEQLARIYTKTNATSYQVTGLTPFTVYSFRLLAVNKLGISPPSKESYYIVTLREAPTGKPIPTTAHNTSSTSVYISWKAPPPDTILGEFLGYRITYRPRDRDPNDTKEIYIRDNTVESHEIQNLQTYTQYKVTVQVFNPEGLGPETTILVMTDEGVPSKPQNLTILDVSANSITMSWHPPKNQNGAIAGYHVFHIHDNQTGVEIVKNSRNSVETLIHFELQNLRPYTDYRVIVKAFTTKNEGEPSDQIAQRTDVGGPSAPAIVNLTCHSQESITIRWRRPYEFYNTIDFYIIKTRLAGQDTHRDIRINASAKELETAMILQNLTTNSYYEVKVAAATFSVINPKKIVLGKFSESRIIQLQPNCEKLQPLLRQSHNDYNLAVLVGIIFSCFGIILIIMAFFLWSRKCFHAAYYYLDDPPHHPNAPQVDWEVPVKIGDEIRAAVPVNEFAKHVASLHADGDIGFSREYEAIQNECISDDLPCEHSQHPENKRKNRYLNITAYDHSRVHLHPTPGQKKNLDYINANFIDGYQKGHAFIGTQGPLPDTFDCFWRMIWEQRVAIIVMITNLVERGRRKCDMYWPKDGVETYGVIQVKLIEEEVMSTYTVRTLQIKHLKLKKKKQCNTEKLVYQYHYTNWPDHGTPDHPLPVLNFVKKSSAANPAEAGPIVVHCSAGVGRTGTYIVLDAMLKQIQQKNIVNVFGFLRHIRAQRNFLVQTEEQYIFLHDALVEAIASGETNLMAEQVEELKNCTPYLEQQYKNIIQFQPKDIHIASAMKQVNSIKNRGAIFPIEGSRVHLTPKPGEDGSDYINASWLHGFRRLRDFIVTQHPMAHTIKDFWQMVWDHNAQTVVLLSSLDDINFAQFWPDEATPIESDHYRVKFLNKTNKSDYVSRDFVIQSIQDDYELTVKMLHCPSWPEMSNPNSIYDFIVDVHERCNDYRNGPIVIVDRYGGAQACTFCAISSLAIEMEYCSTANVYQYAKLYHNKRPGVWTSSEDIRVIYNILSFLPGNLNLLKRTALRTEFEDVTTATPDLYSKICSNVAPLSSSSSTTPPPPSTPTPQPPQTIQLSSHSPSDLSHQISSTVANAGSPATASAGATPTTPMTPTVPPTIPTIPSLASQNSLTLTNANFHTVTNNAADLMEHQQQQMLALMQQQTQLQQQYNTHPQQHHNNVGDLLMNNADNSPTASPTITNNNHITNNNVTSAAATDAQNLDIVG

Target ORF information:

RefSeq Version	NM_143434.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein tyrosine phosphatase 99A, transcript variant F (Ptp99A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143434.5

ORF Insert Sequence:

ATGACGCAGC GCATATCGGC CACATACGGA AATCATCGAG GGCTCATCAT TCCTGGCTTT 
GTGCTGCTCC TGATTTTGGC CACCTTGCCT GCCACGCAGG CAGGACGAAT CTTCGACGTC 
ACAAATCTGG ACTACGTAAA GGTGAATGCC GAGGCGGGCA AGAACGTGAC CATTCCGTGT 
CCGGGGGTCA ACGAGCACTC GCTGGTGGAC ACGCTCGTGT GGAAGACGGC GTCCACGACA 
ATCGCACAGT TTGCCAACCG GATACCCCTG CTCCACAGTC CGAGGATTCA ACTTCTTTCG 
GACAACTTCG GCCTGCAGTT TTCCCCTTCA TTAGCGAGTG ATACCTCGGA ATACATATGC 
CTGGTGAACG ATCGCCATAT ACCAGAAGCC ATTGTCGATT TGCTGGTCCA GGATGTGCCC 
GATCCGCCGG AGCGACCATT GTTGATAAGT TTCACATCAC GCTCGGTGAA TTTATCCTGG 
GCGCACTCAC AGCATCCGCG GAATGCGCCC GTCACGCATT TTATTATCGA AACGCGGGAG 
GGCGAGGATG GCATTTGGGA GCAGCTGGCC AGGATCTATA CGAAGACAAA TGCGACGTCA 
TATCAGGTGA CCGGGCTGAC GCCCTTCACC GTCTACAGCT TTCGTCTGCT GGCGGTCAAC 
AAGCTGGGTA TCAGTCCGCC GTCCAAGGAG TCCTACTACA TAGTCACGCT GCGTGAGGCT 
CCCACGGGCA AGCCCATACC GACGACGGCC CACAACACGT CCTCCACCTC GGTGTACATC 
TCGTGGAAGG CACCGCCGCC GGATACCATA CTGGGCGAAT TCCTTGGCTA CCGCATCACA 
TATCGACCTC GCGATCGGGA CCCCAACGAT ACCAAGGAAA TCTATATACG CGACAACACC 
GTGGAGAGCC ATGAAATTCA AAACCTACAG ACGTACACGC AGTACAAGGT GACAGTGCAG 
GTGTTCAATC CCGAGGGTCT GGGACCGGAG ACCACCATCC TGGTGATGAC CGACGAAGGA 
GTGCCAAGCA AACCGCAGAA TCTAACCATC TTGGATGTTT CGGCCAACAG CATCACGATG 
TCCTGGCATC CGCCAAAGAA TCAAAACGGA GCCATCGCCG GTTACCATGT GTTCCACATA 
CACGACAACC AAACTGGCGT GGAGATCGTC AAGAACTCTC GCAATTCGGT GGAAACCCTC 
ATTCACTTCG AACTGCAGAA CTTACGACCC TACACCGACT ACCGTGTGAT AGTCAAGGCA 
TTCACCACCA AGAACGAGGG CGAACCCTCC GATCAGATTG CCCAGCGTAC GGATGTCGGA 
GGACCCAGTG CCCCGGCGAT TGTGAACCTC ACTTGTCACT CCCAGGAGTC CATCACGATT 
CGATGGCGAA GACCCTATGA GTTCTACAAC ACCATCGACT TTTACATCAT CAAGACGCGT 
TTGGCTGGCC AGGATACGCA TCGGGATATC AGGATTAATG CCTCGGCCAA GGAACTGGAA 
ACTGCGATGA TTCTTCAAAA CCTAACAACC AACTCGTATT ACGAGGTCAA AGTGGCAGCG 
GCAACGTTCA GTGTCATAAA TCCAAAGAAA ATTGTGCTGG GCAAATTCTC GGAGTCAAGG 
ATTATACAAC TGCAACCGAA CTGCGAGAAA TTGCAACCGC TGCTGCGGCA GTCGCATAAT 
GACTACAACC TGGCCGTTCT GGTGGGCATC ATCTTCAGCT GCTTCGGCAT CATCCTGATC 
ATCATGGCCT TCTTCCTGTG GAGCAGAAAG TGCTTCCATG CGGCCTACTA CTATCTGGAC 
GACCCGCCAC ACCATCCAAA TGCCCCGCAG GTGGACTGGG AAGTGCCCGT TAAGATCGGC 
GACGAGATCC GAGCGGCTGT GCCCGTCAAC GAGTTCGCCA AGCACGTGGC TTCCCTGCAC 
GCGGACGGGG ACATTGGATT CAGCCGGGAG TACGAGGCCA TCCAGAACGA GTGCATCAGC 
GATGATCTGC CGTGTGAACA CTCGCAGCAT CCGGAGAACA AACGCAAGAA TCGCTATCTC 
AACATCACGG CCTATGACCA CAGTCGGGTG CATCTGCATC CCACACCGGG ACAGAAGAAG 
AATCTGGACT ACATCAATGC CAACTTCATC GATGGCTACC AGAAGGGACA TGCCTTCATC 
GGCACCCAGG GTCCATTGCC CGACACCTTC GATTGCTTCT GGCGCATGAT CTGGGAACAG 
AGGGTGGCCA TCATCGTGAT GATCACCAAT CTTGTGGAGC GCGGGCGGCG CAAGTGCGAC 
ATGTACTGGC CGAAGGATGG GGTGGAGACC TATGGCGTAA TCCAGGTCAA GCTCATCGAG 
GAGGAGGTCA TGTCCACGTA CACTGTGCGC ACCCTGCAGA TCAAGCACTT GAAGCTCAAG 
AAGAAGAAGC AGTGCAACAC CGAAAAGCTG GTCTATCAAT ACCACTACAC CAACTGGCCC 
GACCACGGAA CACCCGATCA TCCCCTACCC GTGCTTAACT TTGTCAAGAA ATCCTCGGCC 
GCCAATCCCG CAGAGGCTGG TCCCATAGTC GTGCACTGCA GCGCTGGCGT CGGTCGCACT 
GGCACATACA TCGTCCTGGA CGCCATGCTC AAGCAGATCC AGCAGAAGAA CATCGTCAAC 
GTCTTTGGCT TTCTGCGTCA CATTCGTGCT CAGAGGAACT TCCTGGTGCA GACCGAGGAG 
CAGTACATAT TCCTGCACGA CGCTCTGGTG GAGGCCATCG CCTCGGGGGA GACCAATCTG 
ATGGCCGAGC AGGTGGAGGA GCTGAAGAAC TGCACTCCCT ACCTGGAGCA GCAGTACAAG 
AACATCATCC AGTTCCAACC GAAAGACATA CACATTGCAT CCGCCATGAA GCAGGTGAAC 
TCGATCAAGA ACCGCGGCGC CATCTTCCCC ATCGAGGGCA GTCGGGTGCA CTTGACGCCC 
AAGCCGGGCG AGGATGGCAG CGATTATATC AACGCCTCGT GGCTGCACGG CTTCCGGCGA 
CTGCGGGACT TCATAGTCAC CCAACATCCC ATGGCGCACA CGATAAAGGA CTTCTGGCAG 
ATGGTCTGGG ACCACAATGC GCAGACCGTC GTGCTGCTCT CATCACTGGA TGATATAAAC 
TTTGCACAGT TTTGGCCAGA TGAGGCCACG CCCATCGAGA GCGATCACTA TCGCGTCAAG 
TTTCTGAACA AGACCAACAA GAGCGACTAT GTGAGCCGCG ACTTTGTCAT CCAGTCGATA 
CAGGACGACT ACGAGCTGAC GGTCAAGATG CTGCACTGTC CCAGTTGGCC AGAGATGTCC 
AATCCCAACA GCATCTACGA CTTCATCGTC GATGTGCACG AGCGCTGCAA TGACTATCGC 
AATGGACCCA TCGTCATTGT GGATAGATAT GGTGGCGCCC AGGCGTGCAC CTTCTGTGCC 
ATCTCCTCGC TGGCCATCGA GATGGAGTAC TGCAGCACGG CGAATGTGTA CCAGTACGCG 
AAGCTGTACC ACAACAAGCG ACCCGGGGTG TGGACATCCA GCGAGGACAT CCGCGTCATC 
TACAACATAC TTTCATTTTT GCCTGGCAAT TTGAACCTGC TGAAGCGCAC GGCGCTTCGG 
ACTGAATTCG AGGATGTGAC AACGGCCACG CCGGATCTCT ATAGCAAAAT ATGCAGCAAT 
GTCGCTCCTC TATCCTCCAG TTCATCCACC ACACCACCAC CACCAAGTAC TCCAACACCA 
CAGCCCCCAC AAACCATCCA ACTGTCATCG CATTCGCCAT CCGACCTGTC CCACCAGATC 
TCATCCACAG TCGCCAATGC AGGATCACCA GCAACTGCAA GTGCAGGCGC AACACCAACG 
ACTCCAATGA CACCGACAGT CCCACCCACA ATACCTACAA TACCATCACT CGCATCCCAG 
AACAGTCTAA CCCTTACTAA TGCCAATTTC CATACTGTTA CTAATAACGC TGCTGATCTG 
ATGGAACACC AACAACAACA AATGCTGGCC CTGATGCAAC AACAAACGCA ACTGCAACAA 
CAATACAACA CGCACCCGCA ACAACATCAC AACAACGTCG GTGACCTGCT GATGAACAAT 
GCGGACAACT CACCAACCGC TTCACCAACG ATCACCAACA ACAATCATAT AACCAACAAC 
AACGTCACAA GCGCAGCAGC GACAGATGCT CAAAACCTAG ATATTGTAGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptp99A ( NM_206579.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996302.1 Drosophila melanogaster protein tyrosine phosphatase 99A, transcript variant C (Ptp99A), mRNA.

Price & Availability↑

CloneID	OFa06491
Clone ID Related Accession (Same CDS sequence)	NM_206579.3
Accession Version	NM_206579.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein tyrosine phosphatase 99A, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_206579.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCACGCC CCCAGCACCA CGCCCTTCTG AGAGCAATGC TGAAGTTGTT GCTCTTCGCC 
AGCATTGCGG AGCACTGTGC CACCGCCCTG CCCACCAACT CCTCCAACTC GCCATCGTCG 
CCATCGCCCT TCACCGCCGC CTCATTGCCG CCCACCACTG CATCGTCGTC ATCATCACCG 
GCGGTCATTA GTACCAGTTC TTCCGATCGC AATCTGGCCG ATCTAGTCAA TCCGGAGGCC 
GAAACAACCG GATCCGGCTG GGAATCACTT GAGACTGAAT TCAATCTGGC CACCACTGTG 
GACAGCAGCA CCCAGAAGAC GGAAAAGGAG CCGGTGCTGG GCACCGTAGC CACCTCCATT 
GAGCAGCAGG ATCAGCCGCC GGATGTGCCA GCCACCACGT TGGCCTTCGC CAATGCGTTT 
CCGGTTCCGG TGGCCGGCGA AATGGGAAAC GGAAATGGCA ACTATAACGA TGCCACGCCC 
CCGTACGCAG CGGTCGACGA CAATTATGTG CCAAGCAAAC CGCAGAATCT AACCATCTTG 
GATGTTTCGG CCAACAGCAT CACGATGTCC TGGCATCCGC CAAAGAATCA AAACGGAGCC 
ATCGCCGGTT ACCATGTGTT CCACATACAC GACAACCAAA CTGGCGTGGA GATCGTCAAG 
AACTCTCGCA ATTCGGTGGA AACCCTCATT CACTTCGAAC TGCAGAACTT ACGTGAGTTT 
AACTACGTTT TCATTTGGGG CTTATTTGGC GTAAAAGGAC CCTACACCGA CTACCGTGTG 
ATAGTCAAGG CATTCACCAC CAAGAACGAG GGCGAACCCT CCGATCAGAT TGCCCAGCGT 
ACGGATGTCG GAGGACCCAG TGCCCCGGCG ATTGTGAACC TCACTTGTCA CTCCCAGGAG 
TCCATCACGA TTCGATGGCG AAGACCCTAT GAGTTCTACA ACACCATCGA CTTTTACATC 
ATCAAGACGC GTTTGGCTGG CCAGGATACG CATCGGGATA TCAGGATTAA TGCCTCGGCC 
AAGGAACTGG AAACTGCGAT GATTCTTCAA AACCTAACAA CCAACTCGTA TTACGAGGTC 
AAAGTGGCAG CGGCAACGTT CAGTGTCATA AATCCAAAGA AAATTGTGCT GGGCAAATTC 
TCGGAGTCAA GGATTATACA ACTGCAACCG AACTGCGAGA AATTGCAACC GCTGCTGCGG 
CAGTCGCATA ATGACTACAA CCTGGCCGTT CTGGTGGGCA TCATCTTCAG CTGCTTCGGC 
ATCATCCTGA TCATCATGGC CTTCTTCCTG TGGAGCAGAA AGTGCTTCCA TGCGGCCTAC 
TACTATCTGG ACGACCCGCC ACACCATCCA AATGCCCCGC AGGTGGACTG GGAAGTGCCC 
GTTAAGATCG GCGACGAGAT CCGAGCGGCT GTGCCCGTCA ACGAGTTCGC CAAGCACGTG 
GCTTCCCTGC ACGCGGACGG GGACATTGGA TTCAGCCGGG AGTACGAGGC CATCCAGAAC 
GAGTGCATCA GCGATGATCT GCCGTGTGAA CACTCGCAGC ATCCGGAGAA CAAACGCAAG 
AATCGCTATC TCAACATCAC GGCCTATGAC CACAGTCGGG TGCATCTGCA TCCCACACCG 
GGACAGAAGA AGAATCTGGA CTACATCAAT GCCAACTTCA TCGATGGCTA CCAGAAGGGA 
CATGCCTTCA TCGGCACCCA GGGTCCATTG CCCGACACCT TCGATTGCTT CTGGCGCATG 
ATCTGGGAAC AGAGGGTGGC CATCATCGTG ATGATCACCA ATCTTGTGGA GCGCGGGCGG 
CGCAAGTGCG ACATGTACTG GCCGAAGGAT GGGGTGGAGA CCTATGGCGT AATCCAGGTC 
AAGCTCATCG AGGAGGAGGT CATGTCCACG TACACTGTGC GCACCCTGCA GATCAAGCAC 
TTGAAGCTCA AGAAGAAGAA GCAGTGCAAC ACCGAAAAGC TGGTCTATCA ATACCACTAC 
ACCAACTGGC CCGACCACGG AACACCCGAT CATCCCCTAC CCGTGCTTAA CTTTGTCAAG 
AAATCCTCGG CCGCCAATCC CGCAGAGGCT GGTCCCATAG TCGTGCACTG CAGCGCTGGC 
GTCGGTCGCA CTGGCACATA CATCGTCCTG GACGCCATGC TCAAGCAGAT CCAGCAGAAG 
AACATCGTCA ACGTCTTTGG CTTTCTGCGT CACATTCGTG CTCAGAGGAA CTTCCTGGTG 
CAGACCGAGG AGCAGTACAT ATTCCTGCAC GACGCTCTGG TGGAGGCCAT CGCCTCGGGG 
GAGACCAATC TGATGGCCGA GCAGGTGGAG GAGCTGAAGA ACTGCACTCC CTACCTGGAG 
CAGCAGTACA AGAACATCAT CCAGTTCCAA CCGAAAGACA TACACATTGC ATCCGCCATG 
AAGCAGGTGA ACTCGATCAA GAACCGCGGC GCCATCTTCC CCATCGAGGG CAGTCGGGTG 
CACTTGACGC CCAAGCCGGG CGAGGATGGC AGCGATTATA TCAACGCCTC GTGGCTGCAC 
GGCTTCCGGC GACTGCGGGA CTTCATAGTC ACCCAACATC CCATGGCGCA CACGATAAAG 
GACTTCTGGC AGATGGTCTG GGACCACAAT GCGCAGACCG TCGTGCTGCT CTCATCACTG 
GATGATATAA ACTTTGCACA GTTTTGGCCA GATGAGGCCA CGCCCATCGA GAGCGATCAC 
TATCGCGTCA AGTTTCTGAA CAAGACCAAC AAGAGCGACT ATGTGAGCCG CGACTTTGTC 
ATCCAGTCGA TACAGGACGA CTACGAGCTG ACGGTCAAGA TGCTGCACTG TCCCAGTTGG 
CCAGAGATGT CCAATCCCAA CAGCATCTAC GACTTCATCG TCGATGTGCA CGAGCGCTGC 
AATGACTATC GCAATGGACC CATCGTCATT GTGGATAGAT ATGGTGGCGC CCAGGCGTGC 
ACCTTCTGTG CCATCTCCTC GCTGGCCATC GAGATGGAGT ACTGCAGCAC GGCGAATGTG 
TACCAGTACG CGAAGCTGTA CCACAACAAG CGACCCGGGG TGTGGACATC CAGCGAGGAC 
ATCCGCGTCA TCTACAACAT ACTTTCATTT TTGCCTGGCA ATTTGAACCT GCTGAAGCGC 
ACGGCGCTTC GGACTGAATT CGAGGATGTG ACAACGGCCA CGCCGGATCT CTATAGCAAA 
ATATGCAGCA ATGTCGCTCC TCTATCCTCC AGTTCATCCA CCACACCACC ACCACCAAGT 
ACTCCAACAC CACAGCCCCC ACAAACCATC CAACTGTCAT CGCATTCGCC ATCCGACCTG 
TCCCACCAGA TCTCATCCAC AGTCGCCAAT GCAGGATCAC CAGCAACTGC AAGTGCAGGC 
GCAACACCAA CGACTCCAAT GACACCGACA GTCCCACCCA CAATACCTAC AATACCATCA 
CTCGCATCCC AGAACAGTCT AACCCTTACT AATGCCAATT TCCATACTGT TACTAATAAC 
GCTGCTGATC TGATGGAACA CCAACAACAA CAAATGCTGG CCCTGATGCA ACAACAAACG 
CAACTGCAAC AACAATACAA CACGCACCCG CAACAACATC ACAACAACGT CGGTGACCTG 
CTGATGAACA ATGCGGACAA CTCACCAACC GCTTCACCAA CGATCACCAA CAACAATCAT 
ATAACCAACA ACAACGTCAC AAGCGCAGCA GCGACAGATG CTCAAAACCT AGATATTGTA 
GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996302.1
CDS	328..4053
Translation	MPRPQHHALLRAMLKLLLFASIAEHCATALPTNSSNSPSSPSPFTAASLPPTTASSSSSPAVISTSSSDRNLADLVNPEAETTGSGWESLETEFNLATTVDSSTQKTEKEPVLGTVATSIEQQDQPPDVPATTLAFANAFPVPVAGEMGNGNGNYNDATPPYAAVDDNYVPSKPQNLTILDVSANSITMSWHPPKNQNGAIAGYHVFHIHDNQTGVEIVKNSRNSVETLIHFELQNLREFNYVFIWGLFGVKGPYTDYRVIVKAFTTKNEGEPSDQIAQRTDVGGPSAPAIVNLTCHSQESITIRWRRPYEFYNTIDFYIIKTRLAGQDTHRDIRINASAKELETAMILQNLTTNSYYEVKVAAATFSVINPKKIVLGKFSESRIIQLQPNCEKLQPLLRQSHNDYNLAVLVGIIFSCFGIILIIMAFFLWSRKCFHAAYYYLDDPPHHPNAPQVDWEVPVKIGDEIRAAVPVNEFAKHVASLHADGDIGFSREYEAIQNECISDDLPCEHSQHPENKRKNRYLNITAYDHSRVHLHPTPGQKKNLDYINANFIDGYQKGHAFIGTQGPLPDTFDCFWRMIWEQRVAIIVMITNLVERGRRKCDMYWPKDGVETYGVIQVKLIEEEVMSTYTVRTLQIKHLKLKKKKQCNTEKLVYQYHYTNWPDHGTPDHPLPVLNFVKKSSAANPAEAGPIVVHCSAGVGRTGTYIVLDAMLKQIQQKNIVNVFGFLRHIRAQRNFLVQTEEQYIFLHDALVEAIASGETNLMAEQVEELKNCTPYLEQQYKNIIQFQPKDIHIASAMKQVNSIKNRGAIFPIEGSRVHLTPKPGEDGSDYINASWLHGFRRLRDFIVTQHPMAHTIKDFWQMVWDHNAQTVVLLSSLDDINFAQFWPDEATPIESDHYRVKFLNKTNKSDYVSRDFVIQSIQDDYELTVKMLHCPSWPEMSNPNSIYDFIVDVHERCNDYRNGPIVIVDRYGGAQACTFCAISSLAIEMEYCSTANVYQYAKLYHNKRPGVWTSSEDIRVIYNILSFLPGNLNLLKRTALRTEFEDVTTATPDLYSKICSNVAPLSSSSSTTPPPPSTPTPQPPQTIQLSSHSPSDLSHQISSTVANAGSPATASAGATPTTPMTPTVPPTIPTIPSLASQNSLTLTNANFHTVTNNAADLMEHQQQQMLALMQQQTQLQQQYNTHPQQHHNNVGDLLMNNADNSPTASPTITNNNHITNNNVTSAAATDAQNLDIVG

Target ORF information:

RefSeq Version	NM_206579.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein tyrosine phosphatase 99A, transcript variant C (Ptp99A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206579.3

ORF Insert Sequence:

ATGCCACGCC CCCAGCACCA CGCCCTTCTG AGAGCAATGC TGAAGTTGTT GCTCTTCGCC 
AGCATTGCGG AGCACTGTGC CACCGCCCTG CCCACCAACT CCTCCAACTC GCCATCGTCG 
CCATCGCCCT TCACCGCCGC CTCATTGCCG CCCACCACTG CATCGTCGTC ATCATCACCG 
GCGGTCATTA GTACCAGTTC TTCCGATCGC AATCTGGCCG ATCTAGTCAA TCCGGAGGCC 
GAAACAACCG GATCCGGCTG GGAATCACTT GAGACTGAAT TCAATCTGGC CACCACTGTG 
GACAGCAGCA CCCAGAAGAC GGAAAAGGAG CCGGTGCTGG GCACCGTAGC CACCTCCATT 
GAGCAGCAGG ATCAGCCGCC GGATGTGCCA GCCACCACGT TGGCCTTCGC CAATGCGTTT 
CCGGTTCCGG TGGCCGGCGA AATGGGAAAC GGAAATGGCA ACTATAACGA TGCCACGCCC 
CCGTACGCAG CGGTCGACGA CAATTATGTG CCAAGCAAAC CGCAGAATCT AACCATCTTG 
GATGTTTCGG CCAACAGCAT CACGATGTCC TGGCATCCGC CAAAGAATCA AAACGGAGCC 
ATCGCCGGTT ACCATGTGTT CCACATACAC GACAACCAAA CTGGCGTGGA GATCGTCAAG 
AACTCTCGCA ATTCGGTGGA AACCCTCATT CACTTCGAAC TGCAGAACTT ACGTGAGTTT 
AACTACGTTT TCATTTGGGG CTTATTTGGC GTAAAAGGAC CCTACACCGA CTACCGTGTG 
ATAGTCAAGG CATTCACCAC CAAGAACGAG GGCGAACCCT CCGATCAGAT TGCCCAGCGT 
ACGGATGTCG GAGGACCCAG TGCCCCGGCG ATTGTGAACC TCACTTGTCA CTCCCAGGAG 
TCCATCACGA TTCGATGGCG AAGACCCTAT GAGTTCTACA ACACCATCGA CTTTTACATC 
ATCAAGACGC GTTTGGCTGG CCAGGATACG CATCGGGATA TCAGGATTAA TGCCTCGGCC 
AAGGAACTGG AAACTGCGAT GATTCTTCAA AACCTAACAA CCAACTCGTA TTACGAGGTC 
AAAGTGGCAG CGGCAACGTT CAGTGTCATA AATCCAAAGA AAATTGTGCT GGGCAAATTC 
TCGGAGTCAA GGATTATACA ACTGCAACCG AACTGCGAGA AATTGCAACC GCTGCTGCGG 
CAGTCGCATA ATGACTACAA CCTGGCCGTT CTGGTGGGCA TCATCTTCAG CTGCTTCGGC 
ATCATCCTGA TCATCATGGC CTTCTTCCTG TGGAGCAGAA AGTGCTTCCA TGCGGCCTAC 
TACTATCTGG ACGACCCGCC ACACCATCCA AATGCCCCGC AGGTGGACTG GGAAGTGCCC 
GTTAAGATCG GCGACGAGAT CCGAGCGGCT GTGCCCGTCA ACGAGTTCGC CAAGCACGTG 
GCTTCCCTGC ACGCGGACGG GGACATTGGA TTCAGCCGGG AGTACGAGGC CATCCAGAAC 
GAGTGCATCA GCGATGATCT GCCGTGTGAA CACTCGCAGC ATCCGGAGAA CAAACGCAAG 
AATCGCTATC TCAACATCAC GGCCTATGAC CACAGTCGGG TGCATCTGCA TCCCACACCG 
GGACAGAAGA AGAATCTGGA CTACATCAAT GCCAACTTCA TCGATGGCTA CCAGAAGGGA 
CATGCCTTCA TCGGCACCCA GGGTCCATTG CCCGACACCT TCGATTGCTT CTGGCGCATG 
ATCTGGGAAC AGAGGGTGGC CATCATCGTG ATGATCACCA ATCTTGTGGA GCGCGGGCGG 
CGCAAGTGCG ACATGTACTG GCCGAAGGAT GGGGTGGAGA CCTATGGCGT AATCCAGGTC 
AAGCTCATCG AGGAGGAGGT CATGTCCACG TACACTGTGC GCACCCTGCA GATCAAGCAC 
TTGAAGCTCA AGAAGAAGAA GCAGTGCAAC ACCGAAAAGC TGGTCTATCA ATACCACTAC 
ACCAACTGGC CCGACCACGG AACACCCGAT CATCCCCTAC CCGTGCTTAA CTTTGTCAAG 
AAATCCTCGG CCGCCAATCC CGCAGAGGCT GGTCCCATAG TCGTGCACTG CAGCGCTGGC 
GTCGGTCGCA CTGGCACATA CATCGTCCTG GACGCCATGC TCAAGCAGAT CCAGCAGAAG 
AACATCGTCA ACGTCTTTGG CTTTCTGCGT CACATTCGTG CTCAGAGGAA CTTCCTGGTG 
CAGACCGAGG AGCAGTACAT ATTCCTGCAC GACGCTCTGG TGGAGGCCAT CGCCTCGGGG 
GAGACCAATC TGATGGCCGA GCAGGTGGAG GAGCTGAAGA ACTGCACTCC CTACCTGGAG 
CAGCAGTACA AGAACATCAT CCAGTTCCAA CCGAAAGACA TACACATTGC ATCCGCCATG 
AAGCAGGTGA ACTCGATCAA GAACCGCGGC GCCATCTTCC CCATCGAGGG CAGTCGGGTG 
CACTTGACGC CCAAGCCGGG CGAGGATGGC AGCGATTATA TCAACGCCTC GTGGCTGCAC 
GGCTTCCGGC GACTGCGGGA CTTCATAGTC ACCCAACATC CCATGGCGCA CACGATAAAG 
GACTTCTGGC AGATGGTCTG GGACCACAAT GCGCAGACCG TCGTGCTGCT CTCATCACTG 
GATGATATAA ACTTTGCACA GTTTTGGCCA GATGAGGCCA CGCCCATCGA GAGCGATCAC 
TATCGCGTCA AGTTTCTGAA CAAGACCAAC AAGAGCGACT ATGTGAGCCG CGACTTTGTC 
ATCCAGTCGA TACAGGACGA CTACGAGCTG ACGGTCAAGA TGCTGCACTG TCCCAGTTGG 
CCAGAGATGT CCAATCCCAA CAGCATCTAC GACTTCATCG TCGATGTGCA CGAGCGCTGC 
AATGACTATC GCAATGGACC CATCGTCATT GTGGATAGAT ATGGTGGCGC CCAGGCGTGC 
ACCTTCTGTG CCATCTCCTC GCTGGCCATC GAGATGGAGT ACTGCAGCAC GGCGAATGTG 
TACCAGTACG CGAAGCTGTA CCACAACAAG CGACCCGGGG TGTGGACATC CAGCGAGGAC 
ATCCGCGTCA TCTACAACAT ACTTTCATTT TTGCCTGGCA ATTTGAACCT GCTGAAGCGC 
ACGGCGCTTC GGACTGAATT CGAGGATGTG ACAACGGCCA CGCCGGATCT CTATAGCAAA 
ATATGCAGCA ATGTCGCTCC TCTATCCTCC AGTTCATCCA CCACACCACC ACCACCAAGT 
ACTCCAACAC CACAGCCCCC ACAAACCATC CAACTGTCAT CGCATTCGCC ATCCGACCTG 
TCCCACCAGA TCTCATCCAC AGTCGCCAAT GCAGGATCAC CAGCAACTGC AAGTGCAGGC 
GCAACACCAA CGACTCCAAT GACACCGACA GTCCCACCCA CAATACCTAC AATACCATCA 
CTCGCATCCC AGAACAGTCT AACCCTTACT AATGCCAATT TCCATACTGT TACTAATAAC 
GCTGCTGATC TGATGGAACA CCAACAACAA CAAATGCTGG CCCTGATGCA ACAACAAACG 
CAACTGCAAC AACAATACAA CACGCACCCG CAACAACATC ACAACAACGT CGGTGACCTG 
CTGATGAACA ATGCGGACAA CTCACCAACC GCTTCACCAA CGATCACCAA CAACAATCAT 
ATAACCAACA ACAACGTCAC AAGCGCAGCA GCGACAGATG CTCAAAACCT AGATATTGTA 
GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptp99A ( NM_170409.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733288.1 Drosophila melanogaster protein tyrosine phosphatase 99A, transcript variant A (Ptp99A), mRNA.

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CloneID	OFa06492
Clone ID Related Accession (Same CDS sequence)	NM_170409.3
Accession Version	NM_170409.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein tyrosine phosphatase 99A, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170409.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCACGCC CCCAGCACCA CGCCCTTCTG AGAGCAATGC TGAAGTTGTT GCTCTTCGCC 
AGCATTGCGG AGCACTGTGC CACCGCCCTG CCCACCAACT CCTCCAACTC GCCATCGTCG 
CCATCGCCCT TCACCGCCGC CTCATTGCCG CCCACCACTG CATCGTCGTC ATCATCACCG 
GCGGTCATTA GTACCAGTTC TTCCGATCGC AATCTGGCCG ATCTAGTCAA TCCGGAGGCC 
GAAACAACCG GATCCGGCTG GGAATCACTT GAGACTGAAT TCAATCTGGC CACCACTGTG 
GACAGCAGCA CCCAGAAGAC GGAAAAGGAG CCGGTGCTGG GCACCGTAGC CACCTCCATT 
GAGCAGCAGG ATCAGCCGCC GGATGTGCCA GCCACCACGT TGGCCTTCGC CAATGCGTTT 
CCGGTTCCGG TGGCCGGCGA AATGGGAAAC GGAAATGGCA ACTATAACGA TGCCACGCCC 
CCGTACGCAG CGGTCGACGA CAATTATGTG CCAAGCAAAC CGCAGAATCT AACCATCTTG 
GATGTTTCGG CCAACAGCAT CACGATGTCC TGGCATCCGC CAAAGAATCA AAACGGAGCC 
ATCGCCGGTT ACCATGTGTT CCACATACAC GACAACCAAA CTGGCGTGGA GATCGTCAAG 
AACTCTCGCA ATTCGGTGGA AACCCTCATT CACTTCGAAC TGCAGAACTT ACGACCCTAC 
ACCGACTACC GTGTGATAGT CAAGGCATTC ACCACCAAGA ACGAGGGCGA ACCCTCCGAT 
CAGATTGCCC AGCGTACGGA TGTCGGAGGA CCCAGTGCCC CGGCGATTGT GAACCTCACT 
TGTCACTCCC AGGAGTCCAT CACGATTCGA TGGCGAAGAC CCTATGAGTT CTACAACACC 
ATCGACTTTT ACATCATCAA GACGCGTTTG GCTGGCCAGG ATACGCATCG GGATATCAGG 
ATTAATGCCT CGGCCAAGGA ACTGGAAACT GCGATGATTC TTCAAAACCT AACAACCAAC 
TCGTATTACG AGGTCAAAGT GGCAGCGGCA ACGTTCAGTG TCATAAATCC AAAGAAAATT 
GTGCTGGGCA AATTCTCGGA GTCAAGGATT ATACAACTGC AACCGAACTG CGAGAAATTG 
CAACCGCTGC TGCGGCAGTC GCATAATGAC TACAACCTGG CCGTTCTGGT GGGCATCATC 
TTCAGCTGCT TCGGCATCAT CCTGATCATC ATGGCCTTCT TCCTGTGGAG CAGAAAGTGC 
TTCCATGCGG CCTACTACTA TCTGGACGAC CCGCCACACC ATCCAAATGC CCCGCAGGTG 
GACTGGGAAG TGCCCGTTAA GATCGGCGAC GAGATCCGAG CGGCTGTGCC CGTCAACGAG 
TTCGCCAAGC ACGTGGCTTC CCTGCACGCG GACGGGGACA TTGGATTCAG CCGGGAGTAC 
GAGGCCATCC AGAACGAGTG CATCAGCGAT GATCTGCCGT GTGAACACTC GCAGCATCCG 
GAGAACAAAC GCAAGAATCG CTATCTCAAC ATCACGGCCT ATGACCACAG TCGGGTGCAT 
CTGCATCCCA CACCGGGACA GAAGAAGAAT CTGGACTACA TCAATGCCAA CTTCATCGAT 
GGCTACCAGA AGGGACATGC CTTCATCGGC ACCCAGGGTC CATTGCCCGA CACCTTCGAT 
TGCTTCTGGC GCATGATCTG GGAACAGAGG GTGGCCATCA TCGTGATGAT CACCAATCTT 
GTGGAGCGCG GGCGGCGCAA GTGCGACATG TACTGGCCGA AGGATGGGGT GGAGACCTAT 
GGCGTAATCC AGGTCAAGCT CATCGAGGAG GAGGTCATGT CCACGTACAC TGTGCGCACC 
CTGCAGATCA AGCACTTGAA GCTCAAGAAG AAGAAGCAGT GCAACACCGA AAAGCTGGTC 
TATCAATACC ACTACACCAA CTGGCCCGAC CACGGAACAC CCGATCATCC CCTACCCGTG 
CTTAACTTTG TCAAGAAATC CTCGGCCGCC AATCCCGCAG AGGCTGGTCC CATAGTCGTG 
CACTGCAGCG CTGGCGTCGG TCGCACTGGC ACATACATCG TCCTGGACGC CATGCTCAAG 
CAGATCCAGC AGAAGAACAT CGTCAACGTC TTTGGCTTTC TGCGTCACAT TCGTGCTCAG 
AGGAACTTCC TGGTGCAGAC CGAGGAGCAG TACATATTCC TGCACGACGC TCTGGTGGAG 
GCCATCGCCT CGGGGGAGAC CAATCTGATG GCCGAGCAGG TGGAGGAGCT GAAGAACTGC 
ACTCCCTACC TGGAGCAGCA GTACAAGAAC ATCATCCAGT TCCAACCGAA AGACATACAC 
ATTGCATCCG CCATGAAGCA GGTGAACTCG ATCAAGAACC GCGGCGCCAT CTTCCCCATC 
GAGGGCAGTC GGGTGCACTT GACGCCCAAG CCGGGCGAGG ATGGCAGCGA TTATATCAAC 
GCCTCGTGGC TGCACGGCTT CCGGCGACTG CGGGACTTCA TAGTCACCCA ACATCCCATG 
GCGCACACGA TAAAGGACTT CTGGCAGATG GTCTGGGACC ACAATGCGCA GACCGTCGTG 
CTGCTCTCAT CACTGGATGA TATAAACTTT GCACAGTTTT GGCCAGATGA GGCCACGCCC 
ATCGAGAGCG ATCACTATCG CGTCAAGTTT CTGAACAAGA CCAACAAGAG CGACTATGTG 
AGCCGCGACT TTGTCATCCA GTCGATACAG GACGACTACG AGCTGACGGT CAAGATGCTG 
CACTGTCCCA GTTGGCCAGA GATGTCCAAT CCCAACAGCA TCTACGACTT CATCGTCGAT 
GTGCACGAGC GCTGCAATGA CTATCGCAAT GGACCCATCG TCATTGTGGA TAGATATGGT 
GGCGCCCAGG CGTGCACCTT CTGTGCCATC TCCTCGCTGG CCATCGAGAT GGAGTACTGC 
AGCACGGCGA ATGTGTACCA GTACGCGAAG CTGTACCACA ACAAGCGACC CGGGGTGTGG 
ACATCCAGCG AGGACATCCG CGTCATCTAC AACATACTTT CATTTTTGCC TGGCAATTTG 
AACCTGCTGA AGCGCACGGC GCTTCGGACT GAATTCGAGG ATGTGACAAC GGCCACGCCG 
GATCTCTATA GCAAAATATG CAGCAATGTC GCTCCTCTAT CCTCCAGTTC ATCCACCACA 
CCACCACCAC CAAGTACTCC AACACCACAG CCCCCACAAA CCATCCAACT GTCATCGCAT 
TCGCCATCCG ACCTGTCCCA CCAGATCTCA TCCACAGTCG CCAATGCAGG ATCACCAGCA 
ACTGCAAGTG CAGGCGCAAC ACCAACGACT CCAATGACAC CGACAGTCCC ACCCACAATA 
CCTACAATAC CATCACTCGC ATCCCAGAAC AGTCTAACCC TTACTAATGC CAATTTCCAT 
ACTGTTACTA ATAACGCTGC TGATCTGATG GAACACCAAC AACAACAAAT GCTGGCCCTG 
ATGCAACAAC AAACGCAACT GCAACAACAA TACAACACGC ACCCGCAACA ACATCACAAC 
AACGTCGGTG ACCTGCTGAT GAACAATGCG GACAACTCAC CAACCGCTTC ACCAACGATC 
ACCAACAACA ATCATATAAC CAACAACAAC GTCACAAGCG CAGCAGCGAC AGATGCTCAA 
AACCTAGATA TTGTAGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733288.1
CDS	328..4008
Translation	MPRPQHHALLRAMLKLLLFASIAEHCATALPTNSSNSPSSPSPFTAASLPPTTASSSSSPAVISTSSSDRNLADLVNPEAETTGSGWESLETEFNLATTVDSSTQKTEKEPVLGTVATSIEQQDQPPDVPATTLAFANAFPVPVAGEMGNGNGNYNDATPPYAAVDDNYVPSKPQNLTILDVSANSITMSWHPPKNQNGAIAGYHVFHIHDNQTGVEIVKNSRNSVETLIHFELQNLRPYTDYRVIVKAFTTKNEGEPSDQIAQRTDVGGPSAPAIVNLTCHSQESITIRWRRPYEFYNTIDFYIIKTRLAGQDTHRDIRINASAKELETAMILQNLTTNSYYEVKVAAATFSVINPKKIVLGKFSESRIIQLQPNCEKLQPLLRQSHNDYNLAVLVGIIFSCFGIILIIMAFFLWSRKCFHAAYYYLDDPPHHPNAPQVDWEVPVKIGDEIRAAVPVNEFAKHVASLHADGDIGFSREYEAIQNECISDDLPCEHSQHPENKRKNRYLNITAYDHSRVHLHPTPGQKKNLDYINANFIDGYQKGHAFIGTQGPLPDTFDCFWRMIWEQRVAIIVMITNLVERGRRKCDMYWPKDGVETYGVIQVKLIEEEVMSTYTVRTLQIKHLKLKKKKQCNTEKLVYQYHYTNWPDHGTPDHPLPVLNFVKKSSAANPAEAGPIVVHCSAGVGRTGTYIVLDAMLKQIQQKNIVNVFGFLRHIRAQRNFLVQTEEQYIFLHDALVEAIASGETNLMAEQVEELKNCTPYLEQQYKNIIQFQPKDIHIASAMKQVNSIKNRGAIFPIEGSRVHLTPKPGEDGSDYINASWLHGFRRLRDFIVTQHPMAHTIKDFWQMVWDHNAQTVVLLSSLDDINFAQFWPDEATPIESDHYRVKFLNKTNKSDYVSRDFVIQSIQDDYELTVKMLHCPSWPEMSNPNSIYDFIVDVHERCNDYRNGPIVIVDRYGGAQACTFCAISSLAIEMEYCSTANVYQYAKLYHNKRPGVWTSSEDIRVIYNILSFLPGNLNLLKRTALRTEFEDVTTATPDLYSKICSNVAPLSSSSSTTPPPPSTPTPQPPQTIQLSSHSPSDLSHQISSTVANAGSPATASAGATPTTPMTPTVPPTIPTIPSLASQNSLTLTNANFHTVTNNAADLMEHQQQQMLALMQQQTQLQQQYNTHPQQHHNNVGDLLMNNADNSPTASPTITNNNHITNNNVTSAAATDAQNLDIVG

Target ORF information:

RefSeq Version	NM_170409.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein tyrosine phosphatase 99A, transcript variant A (Ptp99A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170409.3

ORF Insert Sequence:

ATGCCACGCC CCCAGCACCA CGCCCTTCTG AGAGCAATGC TGAAGTTGTT GCTCTTCGCC 
AGCATTGCGG AGCACTGTGC CACCGCCCTG CCCACCAACT CCTCCAACTC GCCATCGTCG 
CCATCGCCCT TCACCGCCGC CTCATTGCCG CCCACCACTG CATCGTCGTC ATCATCACCG 
GCGGTCATTA GTACCAGTTC TTCCGATCGC AATCTGGCCG ATCTAGTCAA TCCGGAGGCC 
GAAACAACCG GATCCGGCTG GGAATCACTT GAGACTGAAT TCAATCTGGC CACCACTGTG 
GACAGCAGCA CCCAGAAGAC GGAAAAGGAG CCGGTGCTGG GCACCGTAGC CACCTCCATT 
GAGCAGCAGG ATCAGCCGCC GGATGTGCCA GCCACCACGT TGGCCTTCGC CAATGCGTTT 
CCGGTTCCGG TGGCCGGCGA AATGGGAAAC GGAAATGGCA ACTATAACGA TGCCACGCCC 
CCGTACGCAG CGGTCGACGA CAATTATGTG CCAAGCAAAC CGCAGAATCT AACCATCTTG 
GATGTTTCGG CCAACAGCAT CACGATGTCC TGGCATCCGC CAAAGAATCA AAACGGAGCC 
ATCGCCGGTT ACCATGTGTT CCACATACAC GACAACCAAA CTGGCGTGGA GATCGTCAAG 
AACTCTCGCA ATTCGGTGGA AACCCTCATT CACTTCGAAC TGCAGAACTT ACGACCCTAC 
ACCGACTACC GTGTGATAGT CAAGGCATTC ACCACCAAGA ACGAGGGCGA ACCCTCCGAT 
CAGATTGCCC AGCGTACGGA TGTCGGAGGA CCCAGTGCCC CGGCGATTGT GAACCTCACT 
TGTCACTCCC AGGAGTCCAT CACGATTCGA TGGCGAAGAC CCTATGAGTT CTACAACACC 
ATCGACTTTT ACATCATCAA GACGCGTTTG GCTGGCCAGG ATACGCATCG GGATATCAGG 
ATTAATGCCT CGGCCAAGGA ACTGGAAACT GCGATGATTC TTCAAAACCT AACAACCAAC 
TCGTATTACG AGGTCAAAGT GGCAGCGGCA ACGTTCAGTG TCATAAATCC AAAGAAAATT 
GTGCTGGGCA AATTCTCGGA GTCAAGGATT ATACAACTGC AACCGAACTG CGAGAAATTG 
CAACCGCTGC TGCGGCAGTC GCATAATGAC TACAACCTGG CCGTTCTGGT GGGCATCATC 
TTCAGCTGCT TCGGCATCAT CCTGATCATC ATGGCCTTCT TCCTGTGGAG CAGAAAGTGC 
TTCCATGCGG CCTACTACTA TCTGGACGAC CCGCCACACC ATCCAAATGC CCCGCAGGTG 
GACTGGGAAG TGCCCGTTAA GATCGGCGAC GAGATCCGAG CGGCTGTGCC CGTCAACGAG 
TTCGCCAAGC ACGTGGCTTC CCTGCACGCG GACGGGGACA TTGGATTCAG CCGGGAGTAC 
GAGGCCATCC AGAACGAGTG CATCAGCGAT GATCTGCCGT GTGAACACTC GCAGCATCCG 
GAGAACAAAC GCAAGAATCG CTATCTCAAC ATCACGGCCT ATGACCACAG TCGGGTGCAT 
CTGCATCCCA CACCGGGACA GAAGAAGAAT CTGGACTACA TCAATGCCAA CTTCATCGAT 
GGCTACCAGA AGGGACATGC CTTCATCGGC ACCCAGGGTC CATTGCCCGA CACCTTCGAT 
TGCTTCTGGC GCATGATCTG GGAACAGAGG GTGGCCATCA TCGTGATGAT CACCAATCTT 
GTGGAGCGCG GGCGGCGCAA GTGCGACATG TACTGGCCGA AGGATGGGGT GGAGACCTAT 
GGCGTAATCC AGGTCAAGCT CATCGAGGAG GAGGTCATGT CCACGTACAC TGTGCGCACC 
CTGCAGATCA AGCACTTGAA GCTCAAGAAG AAGAAGCAGT GCAACACCGA AAAGCTGGTC 
TATCAATACC ACTACACCAA CTGGCCCGAC CACGGAACAC CCGATCATCC CCTACCCGTG 
CTTAACTTTG TCAAGAAATC CTCGGCCGCC AATCCCGCAG AGGCTGGTCC CATAGTCGTG 
CACTGCAGCG CTGGCGTCGG TCGCACTGGC ACATACATCG TCCTGGACGC CATGCTCAAG 
CAGATCCAGC AGAAGAACAT CGTCAACGTC TTTGGCTTTC TGCGTCACAT TCGTGCTCAG 
AGGAACTTCC TGGTGCAGAC CGAGGAGCAG TACATATTCC TGCACGACGC TCTGGTGGAG 
GCCATCGCCT CGGGGGAGAC CAATCTGATG GCCGAGCAGG TGGAGGAGCT GAAGAACTGC 
ACTCCCTACC TGGAGCAGCA GTACAAGAAC ATCATCCAGT TCCAACCGAA AGACATACAC 
ATTGCATCCG CCATGAAGCA GGTGAACTCG ATCAAGAACC GCGGCGCCAT CTTCCCCATC 
GAGGGCAGTC GGGTGCACTT GACGCCCAAG CCGGGCGAGG ATGGCAGCGA TTATATCAAC 
GCCTCGTGGC TGCACGGCTT CCGGCGACTG CGGGACTTCA TAGTCACCCA ACATCCCATG 
GCGCACACGA TAAAGGACTT CTGGCAGATG GTCTGGGACC ACAATGCGCA GACCGTCGTG 
CTGCTCTCAT CACTGGATGA TATAAACTTT GCACAGTTTT GGCCAGATGA GGCCACGCCC 
ATCGAGAGCG ATCACTATCG CGTCAAGTTT CTGAACAAGA CCAACAAGAG CGACTATGTG 
AGCCGCGACT TTGTCATCCA GTCGATACAG GACGACTACG AGCTGACGGT CAAGATGCTG 
CACTGTCCCA GTTGGCCAGA GATGTCCAAT CCCAACAGCA TCTACGACTT CATCGTCGAT 
GTGCACGAGC GCTGCAATGA CTATCGCAAT GGACCCATCG TCATTGTGGA TAGATATGGT 
GGCGCCCAGG CGTGCACCTT CTGTGCCATC TCCTCGCTGG CCATCGAGAT GGAGTACTGC 
AGCACGGCGA ATGTGTACCA GTACGCGAAG CTGTACCACA ACAAGCGACC CGGGGTGTGG 
ACATCCAGCG AGGACATCCG CGTCATCTAC AACATACTTT CATTTTTGCC TGGCAATTTG 
AACCTGCTGA AGCGCACGGC GCTTCGGACT GAATTCGAGG ATGTGACAAC GGCCACGCCG 
GATCTCTATA GCAAAATATG CAGCAATGTC GCTCCTCTAT CCTCCAGTTC ATCCACCACA 
CCACCACCAC CAAGTACTCC AACACCACAG CCCCCACAAA CCATCCAACT GTCATCGCAT 
TCGCCATCCG ACCTGTCCCA CCAGATCTCA TCCACAGTCG CCAATGCAGG ATCACCAGCA 
ACTGCAAGTG CAGGCGCAAC ACCAACGACT CCAATGACAC CGACAGTCCC ACCCACAATA 
CCTACAATAC CATCACTCGC ATCCCAGAAC AGTCTAACCC TTACTAATGC CAATTTCCAT 
ACTGTTACTA ATAACGCTGC TGATCTGATG GAACACCAAC AACAACAAAT GCTGGCCCTG 
ATGCAACAAC AAACGCAACT GCAACAACAA TACAACACGC ACCCGCAACA ACATCACAAC 
AACGTCGGTG ACCTGCTGAT GAACAATGCG GACAACTCAC CAACCGCTTC ACCAACGATC 
ACCAACAACA ATCATATAAC CAACAACAAC GTCACAAGCG CAGCAGCGAC AGATGCTCAA 
AACCTAGATA TTGTAGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptp99A ( NM_057468.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476816.1 Drosophila melanogaster protein tyrosine phosphatase 99A, transcript variant B (Ptp99A), mRNA.

Price & Availability↑

CloneID	OFa06493
Clone ID Related Accession (Same CDS sequence)	NM_057468.3
Accession Version	NM_057468.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein tyrosine phosphatase 99A, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_057468.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCACGCC CCCAGCACCA CGCCCTTCTG AGAGCAATGC TGAAGTTGTT GCTCTTCGCC 
AGCATTGCGG AGCACTGTGC CACCGCCCTG CCCACCAACT CCTCCAACTC GCCATCGTCG 
CCATCGCCCT TCACCGCCGC CTCATTGCCG CCCACCACTG CATCGTCGTC ATCATCACCG 
GCGGTCATTA GTACCAGTTC TTCCGATCGC AATCTGGCCG ATCTAGTCAA TCCGGAGGCC 
GAAACAACCG GATCCGGCTG GGAATCACTT GAGACTGAAT TCAATCTGGC CACCACTGTG 
GACAGCAGCA CCCAGAAGAC GGAAAAGGAG CCGGTGCTGG GCACCGTAGC CACCTCCATT 
GAGCAGCAGG ATCAGCCGCC GGATGTGCCA GCCACCACGT TGGCCTTCGC CAATGCGTTT 
CCGGTTCCGG TGGCCGGCGA AATGGGAAAC GGAAATGGCA ACTATAACGA TGCCACGCCC 
CCGTACGCAG CGGTCGACGA CAATTATGTG CCAAGCAAAC CGCAGAATCT AACCATCTTG 
GATGTTTCGG CCAACAGCAT CACGATGTCC TGGCATCCGC CAAAGAATCA AAACGGAGCC 
ATCGCCGGTT ACCATGTGTT CCACATACAC GACAACCAAA CTGGCGTGGA GATCGTCAAG 
AACTCTCGCA ATTCGGTGGA AACCCTCATT CACTTCGAAC TGCAGAACTT ACGACCCTAC 
ACCGACTACC GTGTGATAGT CAAGGCATTC ACCACCAAGA ACGAGGGCGA ACCCTCCGAT 
CAGATTGCCC AGCGTACGGA TGTCGGAGGA CCCAGTGCCC CGGCGATTGT GAACCTCACT 
TGTCACTCCC AGGAGTCCAT CACGATTCGA TGGCGAAGAC CCTATGAGTT CTACAACACC 
ATCGACTTTT ACATCATCAA GACGCGTTTG GCTGGCCAGG ATACGCATCG GGATATCAGG 
ATTAATGCCT CGGCCAAGGA ACTGGAAACT GCGATGATTC TTCAAAACCT AACAACCAAC 
TCGTATTACG AGGTCAAAGT GGCAGCGGCA ACGTTCAGTG TCATAAATCC AAAGAAAATT 
GTGCTGGGCA AATTCTCGGA GTCAAGGATT ATACAACTGC AACCGAACTG CGAGAAATTG 
CAACCGCTGC TGCGGCAGTC GCATAATGAC TACAACCTGG CCGTTCTGGT GGGCATCATC 
TTCAGCTGCT TCGGCATCAT CCTGATCATC ATGGCCTTCT TCCTGTGGAG CAGAAAGTGC 
TTCCATGCGG CCTACTACTA TCTGGACGAC CCGCCACACC ATCCAAATGC CCCGCAGGTG 
GACTGGGAAG TGCCCGTTAA GATCGGCGAC GAGATCCGAG CGGCTGTGCC CGTCAACGAG 
TTCGCCAAGC ACGTGGCTTC CCTGCACGCG GACGGGGACA TTGGATTCAG CCGGGAGTAC 
GAGGCCATCC AGAACGAGTG CATCAGCGAT GATCTGCCGT GTGAACACTC GCAGCATCCG 
GAGAACAAAC GCAAGAATCG CTATCTCAAC ATCACGGCCT ATGACCACAG TCGGGTGCAT 
CTGCATCCCA CACCGGGACA GAAGAAGAAT CTGGACTACA TCAATGCCAA CTTCATCGAT 
GGCTACCAGA AGGGACATGC CTTCATCGGC ACCCAGGGTC CATTGCCCGA CACCTTCGAT 
TGCTTCTGGC GCATGATCTG GGAACAGAGG GTGGCCATCA TCGTGATGAT CACCAATCTT 
GTGGAGCGCG GGCGGCGCAA GTGCGACATG TACTGGCCGA AGGATGGGGT GGAGACCTAT 
GGCGTAATCC AGGTCAAGCT CATCGAGGAG GAGGTCATGT CCACGTACAC TGTGCGCACC 
CTGCAGATCA AGCACTTGAA GCTCAAGAAG AAGAAGCAGT GCAACACCGA AAAGCTGGTC 
TATCAATACC ACTACACCAA CTGGCCCGAC CACGGAACAC CCGATCATCC CCTACCCGTG 
CTTAACTTTG TCAAGAAATC CTCGGCCGCC AATCCCGCAG AGGCTGGTCC CATAGTCGTG 
CACTGCAGCG CTGGCGTCGG TCGCACTGGC ACATACATCG TCCTGGACGC CATGCTCAAG 
CAGATCCAGC AGAAGAACAT CGTCAACGTC TTTGGCTTTC TGCGTCACAT TCGTGCTCAG 
AGGAACTTCC TGGTGCAGAC CGAGGAGCAG TACATATTCC TGCACGACGC TCTGGTGGAG 
GCCATCGCCT CGGGGGAGAC CAATCTGATG GCCGAGCAGG TGGAGGAGCT GAAGAACTGC 
ACTCCCTACC TGGAGCAGCA GTACAAGAAC ATCATCCAGT TCCAACCGAA AGACATACAC 
ATTGCATCCG CCATGAAGCA GGTGAACTCG ATCAAGAACC GCGGCGCCAT CTTCCCCATC 
GAGGGCAGTC GGGTGCACTT GACGCCCAAG CCGGGCGAGG ATGGCAGCGA TTATATCAAC 
GCCTCGTGGC TGCACGGCTT CCGGCGACTG CGGGACTTCA TAGTCACCCA ACATCCCATG 
GCGCACACGA TAAAGGACTT CTGGCAGATG GTCTGGGACC ACAATGCGCA GACCGTCGTG 
CTGCTCTCAT CACTGGATGA TATAAACTTT GCACAGTTTT GGCCAGATGA GGCCACGCCC 
ATCGAGAGCG ATCACTATCG CGTCAAGTTT CTGAACAAGA CCAACAAGAG CGACTATGTG 
AGCCGCGACT TTGTCATCCA GTCGATACAG GACGACTACG AGCTGACGGT CAAGATGCTG 
CACTGTCCCA GTTGGCCAGA GATGTCCAAT CCCAACAGCA TCTACGACTT CATCGTCGAT 
GTGCACGAGC GCTGCAATGA CTATCGCAAT GGACCCATCG TCATTGTGGA TAGATATGGT 
GGCGCCCAGG CGTGCACCTT CTGTGCCATC TCCTCGCTGG CCATCGAGAT GGAGTACTGC 
AGCACGGCGA ATGTGTACCA GTACGCGAAG CTGTACCACA ACAAGCGACC CGGGGTGTGG 
ACATCCAGCG AGGACATCCG CGTCATCTAC AACATACTTT CATTTTTGCC TGGCAATTTG 
AACCTGCTGA AGCGCACGGC GCTTCGGACT GAATTCGAGG ATGTGACAAC GGCCACGCCG 
GATCTCTATA GCAAAATATG CAGCAATGGT AATGTTCCAC AGCATGTCAT ACTGCAGCAG 
CAGCAGCTGC ACATGCTGCA GTTGCAGCAG CAACACCTAG AAACACAGCA GCAGCAGCAG 
CAGCAACAGC AGCAGCAGCA GCAGCAGCAA CAACAGACAG CACTCAATGA GACAGTCAGC 
ACACCAAGCA CCGATACTAA TCCAAGCCTC TTGCCCATTC TGTCATTGCT ACCGCCCACA 
GTCGCTCCTC TATCCTCCAG TTCATCCACC ACACCACCAC CACCAAGTAC TCCAACACCA 
CAGCCCCCAC AAACCATCCA ACTGTCATCG CATTCGCCAT CCGACCTGTC CCACCAGATC 
TCATCCACAG TCGCCAATGC AGGATCACCA GCAACTGCAA GTGCAGGCGC AACACCAACG 
ACTCCAATGA CACCGACAGT CCCACCCACA ATACCTACAA TACCATCACT CGCATCCCAG 
AACAGTCTAA CCCTTACTAA TGCCAATTTC CATACTGTTA CTAATAACGC TGCTGATCTG 
ATGGAACACC AACAACAACA AATGCTGGCC CTGATGCAAC AACAAACGCA ACTGCAACAA 
CAATACAACA CGCACCCGCA ACAACATCAC AACAACGTCG GTGACCTGCT GATGAACAAT 
GCGGACAACT CACCAACCGC TTCACCAACG ATCACCAACA ACAATCATAT AACCAACAAC 
AACGTCACAA GCGCAGCAGC GACAGATGCT CAAAACCTAG ATATTGTAGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476816.1
CDS	197..4090
Translation	MPRPQHHALLRAMLKLLLFASIAEHCATALPTNSSNSPSSPSPFTAASLPPTTASSSSSPAVISTSSSDRNLADLVNPEAETTGSGWESLETEFNLATTVDSSTQKTEKEPVLGTVATSIEQQDQPPDVPATTLAFANAFPVPVAGEMGNGNGNYNDATPPYAAVDDNYVPSKPQNLTILDVSANSITMSWHPPKNQNGAIAGYHVFHIHDNQTGVEIVKNSRNSVETLIHFELQNLRPYTDYRVIVKAFTTKNEGEPSDQIAQRTDVGGPSAPAIVNLTCHSQESITIRWRRPYEFYNTIDFYIIKTRLAGQDTHRDIRINASAKELETAMILQNLTTNSYYEVKVAAATFSVINPKKIVLGKFSESRIIQLQPNCEKLQPLLRQSHNDYNLAVLVGIIFSCFGIILIIMAFFLWSRKCFHAAYYYLDDPPHHPNAPQVDWEVPVKIGDEIRAAVPVNEFAKHVASLHADGDIGFSREYEAIQNECISDDLPCEHSQHPENKRKNRYLNITAYDHSRVHLHPTPGQKKNLDYINANFIDGYQKGHAFIGTQGPLPDTFDCFWRMIWEQRVAIIVMITNLVERGRRKCDMYWPKDGVETYGVIQVKLIEEEVMSTYTVRTLQIKHLKLKKKKQCNTEKLVYQYHYTNWPDHGTPDHPLPVLNFVKKSSAANPAEAGPIVVHCSAGVGRTGTYIVLDAMLKQIQQKNIVNVFGFLRHIRAQRNFLVQTEEQYIFLHDALVEAIASGETNLMAEQVEELKNCTPYLEQQYKNIIQFQPKDIHIASAMKQVNSIKNRGAIFPIEGSRVHLTPKPGEDGSDYINASWLHGFRRLRDFIVTQHPMAHTIKDFWQMVWDHNAQTVVLLSSLDDINFAQFWPDEATPIESDHYRVKFLNKTNKSDYVSRDFVIQSIQDDYELTVKMLHCPSWPEMSNPNSIYDFIVDVHERCNDYRNGPIVIVDRYGGAQACTFCAISSLAIEMEYCSTANVYQYAKLYHNKRPGVWTSSEDIRVIYNILSFLPGNLNLLKRTALRTEFEDVTTATPDLYSKICSNGNVPQHVILQQQQLHMLQLQQQHLETQQQQQQQQQQQQQQQQQTALNETVSTPSTDTNPSLLPILSLLPPTVAPLSSSSSTTPPPPSTPTPQPPQTIQLSSHSPSDLSHQISSTVANAGSPATASAGATPTTPMTPTVPPTIPTIPSLASQNSLTLTNANFHTVTNNAADLMEHQQQQMLALMQQQTQLQQQYNTHPQQHHNNVGDLLMNNADNSPTASPTITNNNHITNNNVTSAAATDAQNLDIVG

Target ORF information:

RefSeq Version	NM_057468.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein tyrosine phosphatase 99A, transcript variant B (Ptp99A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057468.3

ORF Insert Sequence:

ATGCCACGCC CCCAGCACCA CGCCCTTCTG AGAGCAATGC TGAAGTTGTT GCTCTTCGCC 
AGCATTGCGG AGCACTGTGC CACCGCCCTG CCCACCAACT CCTCCAACTC GCCATCGTCG 
CCATCGCCCT TCACCGCCGC CTCATTGCCG CCCACCACTG CATCGTCGTC ATCATCACCG 
GCGGTCATTA GTACCAGTTC TTCCGATCGC AATCTGGCCG ATCTAGTCAA TCCGGAGGCC 
GAAACAACCG GATCCGGCTG GGAATCACTT GAGACTGAAT TCAATCTGGC CACCACTGTG 
GACAGCAGCA CCCAGAAGAC GGAAAAGGAG CCGGTGCTGG GCACCGTAGC CACCTCCATT 
GAGCAGCAGG ATCAGCCGCC GGATGTGCCA GCCACCACGT TGGCCTTCGC CAATGCGTTT 
CCGGTTCCGG TGGCCGGCGA AATGGGAAAC GGAAATGGCA ACTATAACGA TGCCACGCCC 
CCGTACGCAG CGGTCGACGA CAATTATGTG CCAAGCAAAC CGCAGAATCT AACCATCTTG 
GATGTTTCGG CCAACAGCAT CACGATGTCC TGGCATCCGC CAAAGAATCA AAACGGAGCC 
ATCGCCGGTT ACCATGTGTT CCACATACAC GACAACCAAA CTGGCGTGGA GATCGTCAAG 
AACTCTCGCA ATTCGGTGGA AACCCTCATT CACTTCGAAC TGCAGAACTT ACGACCCTAC 
ACCGACTACC GTGTGATAGT CAAGGCATTC ACCACCAAGA ACGAGGGCGA ACCCTCCGAT 
CAGATTGCCC AGCGTACGGA TGTCGGAGGA CCCAGTGCCC CGGCGATTGT GAACCTCACT 
TGTCACTCCC AGGAGTCCAT CACGATTCGA TGGCGAAGAC CCTATGAGTT CTACAACACC 
ATCGACTTTT ACATCATCAA GACGCGTTTG GCTGGCCAGG ATACGCATCG GGATATCAGG 
ATTAATGCCT CGGCCAAGGA ACTGGAAACT GCGATGATTC TTCAAAACCT AACAACCAAC 
TCGTATTACG AGGTCAAAGT GGCAGCGGCA ACGTTCAGTG TCATAAATCC AAAGAAAATT 
GTGCTGGGCA AATTCTCGGA GTCAAGGATT ATACAACTGC AACCGAACTG CGAGAAATTG 
CAACCGCTGC TGCGGCAGTC GCATAATGAC TACAACCTGG CCGTTCTGGT GGGCATCATC 
TTCAGCTGCT TCGGCATCAT CCTGATCATC ATGGCCTTCT TCCTGTGGAG CAGAAAGTGC 
TTCCATGCGG CCTACTACTA TCTGGACGAC CCGCCACACC ATCCAAATGC CCCGCAGGTG 
GACTGGGAAG TGCCCGTTAA GATCGGCGAC GAGATCCGAG CGGCTGTGCC CGTCAACGAG 
TTCGCCAAGC ACGTGGCTTC CCTGCACGCG GACGGGGACA TTGGATTCAG CCGGGAGTAC 
GAGGCCATCC AGAACGAGTG CATCAGCGAT GATCTGCCGT GTGAACACTC GCAGCATCCG 
GAGAACAAAC GCAAGAATCG CTATCTCAAC ATCACGGCCT ATGACCACAG TCGGGTGCAT 
CTGCATCCCA CACCGGGACA GAAGAAGAAT CTGGACTACA TCAATGCCAA CTTCATCGAT 
GGCTACCAGA AGGGACATGC CTTCATCGGC ACCCAGGGTC CATTGCCCGA CACCTTCGAT 
TGCTTCTGGC GCATGATCTG GGAACAGAGG GTGGCCATCA TCGTGATGAT CACCAATCTT 
GTGGAGCGCG GGCGGCGCAA GTGCGACATG TACTGGCCGA AGGATGGGGT GGAGACCTAT 
GGCGTAATCC AGGTCAAGCT CATCGAGGAG GAGGTCATGT CCACGTACAC TGTGCGCACC 
CTGCAGATCA AGCACTTGAA GCTCAAGAAG AAGAAGCAGT GCAACACCGA AAAGCTGGTC 
TATCAATACC ACTACACCAA CTGGCCCGAC CACGGAACAC CCGATCATCC CCTACCCGTG 
CTTAACTTTG TCAAGAAATC CTCGGCCGCC AATCCCGCAG AGGCTGGTCC CATAGTCGTG 
CACTGCAGCG CTGGCGTCGG TCGCACTGGC ACATACATCG TCCTGGACGC CATGCTCAAG 
CAGATCCAGC AGAAGAACAT CGTCAACGTC TTTGGCTTTC TGCGTCACAT TCGTGCTCAG 
AGGAACTTCC TGGTGCAGAC CGAGGAGCAG TACATATTCC TGCACGACGC TCTGGTGGAG 
GCCATCGCCT CGGGGGAGAC CAATCTGATG GCCGAGCAGG TGGAGGAGCT GAAGAACTGC 
ACTCCCTACC TGGAGCAGCA GTACAAGAAC ATCATCCAGT TCCAACCGAA AGACATACAC 
ATTGCATCCG CCATGAAGCA GGTGAACTCG ATCAAGAACC GCGGCGCCAT CTTCCCCATC 
GAGGGCAGTC GGGTGCACTT GACGCCCAAG CCGGGCGAGG ATGGCAGCGA TTATATCAAC 
GCCTCGTGGC TGCACGGCTT CCGGCGACTG CGGGACTTCA TAGTCACCCA ACATCCCATG 
GCGCACACGA TAAAGGACTT CTGGCAGATG GTCTGGGACC ACAATGCGCA GACCGTCGTG 
CTGCTCTCAT CACTGGATGA TATAAACTTT GCACAGTTTT GGCCAGATGA GGCCACGCCC 
ATCGAGAGCG ATCACTATCG CGTCAAGTTT CTGAACAAGA CCAACAAGAG CGACTATGTG 
AGCCGCGACT TTGTCATCCA GTCGATACAG GACGACTACG AGCTGACGGT CAAGATGCTG 
CACTGTCCCA GTTGGCCAGA GATGTCCAAT CCCAACAGCA TCTACGACTT CATCGTCGAT 
GTGCACGAGC GCTGCAATGA CTATCGCAAT GGACCCATCG TCATTGTGGA TAGATATGGT 
GGCGCCCAGG CGTGCACCTT CTGTGCCATC TCCTCGCTGG CCATCGAGAT GGAGTACTGC 
AGCACGGCGA ATGTGTACCA GTACGCGAAG CTGTACCACA ACAAGCGACC CGGGGTGTGG 
ACATCCAGCG AGGACATCCG CGTCATCTAC AACATACTTT CATTTTTGCC TGGCAATTTG 
AACCTGCTGA AGCGCACGGC GCTTCGGACT GAATTCGAGG ATGTGACAAC GGCCACGCCG 
GATCTCTATA GCAAAATATG CAGCAATGGT AATGTTCCAC AGCATGTCAT ACTGCAGCAG 
CAGCAGCTGC ACATGCTGCA GTTGCAGCAG CAACACCTAG AAACACAGCA GCAGCAGCAG 
CAGCAACAGC AGCAGCAGCA GCAGCAGCAA CAACAGACAG CACTCAATGA GACAGTCAGC 
ACACCAAGCA CCGATACTAA TCCAAGCCTC TTGCCCATTC TGTCATTGCT ACCGCCCACA 
GTCGCTCCTC TATCCTCCAG TTCATCCACC ACACCACCAC CACCAAGTAC TCCAACACCA 
CAGCCCCCAC AAACCATCCA ACTGTCATCG CATTCGCCAT CCGACCTGTC CCACCAGATC 
TCATCCACAG TCGCCAATGC AGGATCACCA GCAACTGCAA GTGCAGGCGC AACACCAACG 
ACTCCAATGA CACCGACAGT CCCACCCACA ATACCTACAA TACCATCACT CGCATCCCAG 
AACAGTCTAA CCCTTACTAA TGCCAATTTC CATACTGTTA CTAATAACGC TGCTGATCTG 
ATGGAACACC AACAACAACA AATGCTGGCC CTGATGCAAC AACAAACGCA ACTGCAACAA 
CAATACAACA CGCACCCGCA ACAACATCAC AACAACGTCG GTGACCTGCT GATGAACAAT 
GCGGACAACT CACCAACCGC TTCACCAACG ATCACCAACA ACAATCATAT AACCAACAAC 
AACGTCACAA GCGCAGCAGC GACAGATGCT CAAAACCTAG ATATTGTAGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptp99A ( NM_001202380.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189309.1 Drosophila melanogaster protein tyrosine phosphatase 99A, transcript variant G (Ptp99A), mRNA.

Price & Availability↑

CloneID	OFa08950
Clone ID Related Accession (Same CDS sequence)	NM_001202380.2
Accession Version	NM_001202380.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein tyrosine phosphatase 99A, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001202380.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTCAG GTGGCGGGGA AAAGCGCTGC AGCAGCCCCA TGCTGCATGC CACATCGGAA 
ATCGGTGGTG CAACTACTGC AACGCCGCGC CTGCTGCGCC GCAAGTCGCA GCGCGGAAAT 
TGGTTTACAA AGTGCTTCCA TGCGGCCTAC TACTATCTGG ACGACCCGCC ACACCATCCA 
AATGCCCCGC AGGTGGACTG GGAAGTGCCC GTTAAGATCG GCGACGAGAT CCGAGCGGCT 
GTGCCCGTCA ACGAGTTCGC CAAGCACGTG GCTTCCCTGC ACGCGGACGG GGACATTGGA 
TTCAGCCGGG AGTACGAGGC CATCCAGAAC GAGTGCATCA GCGATGATCT GCCGTGTGAA 
CACTCGCAGC ATCCGGAGAA CAAACGCAAG AATCGCTATC TCAACATCAC GGCCTATGAC 
CACAGTCGGG TGCATCTGCA TCCCACACCG GGACAGAAGA AGAATCTGGA CTACATCAAT 
GCCAACTTCA TCGATGGCTA CCAGAAGGGA CATGCCTTCA TCGGCACCCA GGGTCCATTG 
CCCGACACCT TCGATTGCTT CTGGCGCATG ATCTGGGAAC AGAGGGTGGC CATCATCGTG 
ATGATCACCA ATCTTGTGGA GCGCGGGCGG CGCAAGTGCG ACATGTACTG GCCGAAGGAT 
GGGGTGGAGA CCTATGGCGT AATCCAGGTC AAGCTCATCG AGGAGGAGGT CATGTCCACG 
TACACTGTGC GCACCCTGCA GATCAAGCAC TTGAAGCTCA AGAAGAAGAA GCAGTGCAAC 
ACCGAAAAGC TGGTCTATCA ATACCACTAC ACCAACTGGC CCGACCACGG AACACCCGAT 
CATCCCCTAC CCGTGCTTAA CTTTGTCAAG AAATCCTCGG CCGCCAATCC CGCAGAGGCT 
GGTCCCATAG TCGTGCACTG CAGCGCTGGC GTCGGTCGCA CTGGCACATA CATCGTCCTG 
GACGCCATGC TCAAGCAGAT CCAGCAGAAG AACATCGTCA ACGTCTTTGG CTTTCTGCGT 
CACATTCGTG CTCAGAGGAA CTTCCTGGTG CAGACCGAGG AGCAGTACAT ATTCCTGCAC 
GACGCTCTGG TGGAGGCCAT CGCCTCGGGG GAGACCAATC TGATGGCCGA GCAGGTGGAG 
GAGCTGAAGA ACTGCACTCC CTACCTGGAG CAGCAGTACA AGAACATCAT CCAGTTCCAA 
CCGAAAGACA TACACATTGC ATCCGCCATG AAGCAGGTGA ACTCGATCAA GAACCGCGGC 
GCCATCTTCC CCATCGAGGG CAGTCGGGTG CACTTGACGC CCAAGCCGGG CGAGGATGGC 
AGCGATTATA TCAACGCCTC GTGGCTGCAC GGCTTCCGGC GACTGCGGGA CTTCATAGTC 
ACCCAACATC CCATGGCGCA CACGATAAAG GACTTCTGGC AGATGGTCTG GGACCACAAT 
GCGCAGACCG TCGTGCTGCT CTCATCACTG GATGATATAA ACTTTGCACA GTTTTGGCCA 
GATGAGGCCA CGCCCATCGA GAGCGATCAC TATCGCGTCA AGTTTCTGAA CAAGACCAAC 
AAGAGCGACT ATGTGAGCCG CGACTTTGTC ATCCAGTCGA TACAGGACGA CTACGAGCTG 
ACGGTCAAGA TGCTGCACTG TCCCAGTTGG CCAGAGATGT CCAATCCCAA CAGCATCTAC 
GACTTCATCG TCGATGTGCA CGAGCGCTGC AATGACTATC GCAATGGACC CATCGTCATT 
GTGGATAGAT ATGGTGGCGC CCAGGCGTGC ACCTTCTGTG CCATCTCCTC GCTGGCCATC 
GAGATGGAGT ACTGCAGCAC GGCGAATGTG TACCAGTACG CGAAGCTGTA CCACAACAAG 
CGACCCGGGG TGTGGACATC CAGCGAGGAC ATCCGCGTCA TCTACAACAT ACTTTCATTT 
TTGCCTGGCA ATTTGAACCT GCTGAAGCGC ACGGCGCTTC GGACTGAATT CGAGGATGTG 
ACAACGGCCA CGCCGGATCT CTATAGCAAA ATATGCAGCA ATGGTAATGT TCCACAGCAT 
GTCATACTGC AGCAGCAGCA GCTGCACATG CTGCAGTTGC AGCAGCAACA CCTAGAAACA 
CAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAGCAGCAGC AGCAACAACA GACAGCACTC 
AATGAGACAG TCAGCACACC AAGCACCGAT ACTAATCCAA GCCTCTTGCC CATTCTGTCA 
TTGCTACCGC CCACAGTCGC TCCTCTATCC TCCAGTTCAT CCACCACACC ACCACCACCA 
AGTACTCCAA CACCACAGCC CCCACAAACC ATCCAACTGT CATCGCATTC GCCATCCGAC 
CTGTCCCACC AGATCTCATC CACAGTCGCC AATGCAGGAT CACCAGCAAC TGCAAGTGCA 
GGCGCAACAC CAACGACTCC AATGACACCG ACAGTCCCAC CCACAATACC TACAATACCA 
TCACTCGCAT CCCAGAACAG TCTAACCCTT ACTAATGCCA ATTTCCATAC TGTTACTAAT 
AACGCTGCTG ATCTGATGGA ACACCAACAA CAACAAATGC TGGCCCTGAT GCAACAACAA 
ACGCAACTGC AACAACAATA CAACACGCAC CCGCAACAAC ATCACAACAA CGTCGGTGAC 
CTGCTGATGA ACAATGCGGA CAACTCACCA ACCGCTTCAC CAACGATCAC CAACAACAAT 
CATATAACCA ACAACAACGT CACAAGCGCA GCAGCGACAG ATGCTCAAAA CCTAGATATT 
GTAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189309.1
CDS	383..3151
Translation	MSSGGGEKRCSSPMLHATSEIGGATTATPRLLRRKSQRGNWFTKCFHAAYYYLDDPPHHPNAPQVDWEVPVKIGDEIRAAVPVNEFAKHVASLHADGDIGFSREYEAIQNECISDDLPCEHSQHPENKRKNRYLNITAYDHSRVHLHPTPGQKKNLDYINANFIDGYQKGHAFIGTQGPLPDTFDCFWRMIWEQRVAIIVMITNLVERGRRKCDMYWPKDGVETYGVIQVKLIEEEVMSTYTVRTLQIKHLKLKKKKQCNTEKLVYQYHYTNWPDHGTPDHPLPVLNFVKKSSAANPAEAGPIVVHCSAGVGRTGTYIVLDAMLKQIQQKNIVNVFGFLRHIRAQRNFLVQTEEQYIFLHDALVEAIASGETNLMAEQVEELKNCTPYLEQQYKNIIQFQPKDIHIASAMKQVNSIKNRGAIFPIEGSRVHLTPKPGEDGSDYINASWLHGFRRLRDFIVTQHPMAHTIKDFWQMVWDHNAQTVVLLSSLDDINFAQFWPDEATPIESDHYRVKFLNKTNKSDYVSRDFVIQSIQDDYELTVKMLHCPSWPEMSNPNSIYDFIVDVHERCNDYRNGPIVIVDRYGGAQACTFCAISSLAIEMEYCSTANVYQYAKLYHNKRPGVWTSSEDIRVIYNILSFLPGNLNLLKRTALRTEFEDVTTATPDLYSKICSNGNVPQHVILQQQQLHMLQLQQQHLETQQQQQQQQQQQQQQQQQTALNETVSTPSTDTNPSLLPILSLLPPTVAPLSSSSSTTPPPPSTPTPQPPQTIQLSSHSPSDLSHQISSTVANAGSPATASAGATPTTPMTPTVPPTIPTIPSLASQNSLTLTNANFHTVTNNAADLMEHQQQQMLALMQQQTQLQQQYNTHPQQHHNNVGDLLMNNADNSPTASPTITNNNHITNNNVTSAAATDAQNLDIVG

Target ORF information:

RefSeq Version	NM_001202380.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein tyrosine phosphatase 99A, transcript variant G (Ptp99A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202380.2

ORF Insert Sequence:

ATGTCGTCAG GTGGCGGGGA AAAGCGCTGC AGCAGCCCCA TGCTGCATGC CACATCGGAA 
ATCGGTGGTG CAACTACTGC AACGCCGCGC CTGCTGCGCC GCAAGTCGCA GCGCGGAAAT 
TGGTTTACAA AGTGCTTCCA TGCGGCCTAC TACTATCTGG ACGACCCGCC ACACCATCCA 
AATGCCCCGC AGGTGGACTG GGAAGTGCCC GTTAAGATCG GCGACGAGAT CCGAGCGGCT 
GTGCCCGTCA ACGAGTTCGC CAAGCACGTG GCTTCCCTGC ACGCGGACGG GGACATTGGA 
TTCAGCCGGG AGTACGAGGC CATCCAGAAC GAGTGCATCA GCGATGATCT GCCGTGTGAA 
CACTCGCAGC ATCCGGAGAA CAAACGCAAG AATCGCTATC TCAACATCAC GGCCTATGAC 
CACAGTCGGG TGCATCTGCA TCCCACACCG GGACAGAAGA AGAATCTGGA CTACATCAAT 
GCCAACTTCA TCGATGGCTA CCAGAAGGGA CATGCCTTCA TCGGCACCCA GGGTCCATTG 
CCCGACACCT TCGATTGCTT CTGGCGCATG ATCTGGGAAC AGAGGGTGGC CATCATCGTG 
ATGATCACCA ATCTTGTGGA GCGCGGGCGG CGCAAGTGCG ACATGTACTG GCCGAAGGAT 
GGGGTGGAGA CCTATGGCGT AATCCAGGTC AAGCTCATCG AGGAGGAGGT CATGTCCACG 
TACACTGTGC GCACCCTGCA GATCAAGCAC TTGAAGCTCA AGAAGAAGAA GCAGTGCAAC 
ACCGAAAAGC TGGTCTATCA ATACCACTAC ACCAACTGGC CCGACCACGG AACACCCGAT 
CATCCCCTAC CCGTGCTTAA CTTTGTCAAG AAATCCTCGG CCGCCAATCC CGCAGAGGCT 
GGTCCCATAG TCGTGCACTG CAGCGCTGGC GTCGGTCGCA CTGGCACATA CATCGTCCTG 
GACGCCATGC TCAAGCAGAT CCAGCAGAAG AACATCGTCA ACGTCTTTGG CTTTCTGCGT 
CACATTCGTG CTCAGAGGAA CTTCCTGGTG CAGACCGAGG AGCAGTACAT ATTCCTGCAC 
GACGCTCTGG TGGAGGCCAT CGCCTCGGGG GAGACCAATC TGATGGCCGA GCAGGTGGAG 
GAGCTGAAGA ACTGCACTCC CTACCTGGAG CAGCAGTACA AGAACATCAT CCAGTTCCAA 
CCGAAAGACA TACACATTGC ATCCGCCATG AAGCAGGTGA ACTCGATCAA GAACCGCGGC 
GCCATCTTCC CCATCGAGGG CAGTCGGGTG CACTTGACGC CCAAGCCGGG CGAGGATGGC 
AGCGATTATA TCAACGCCTC GTGGCTGCAC GGCTTCCGGC GACTGCGGGA CTTCATAGTC 
ACCCAACATC CCATGGCGCA CACGATAAAG GACTTCTGGC AGATGGTCTG GGACCACAAT 
GCGCAGACCG TCGTGCTGCT CTCATCACTG GATGATATAA ACTTTGCACA GTTTTGGCCA 
GATGAGGCCA CGCCCATCGA GAGCGATCAC TATCGCGTCA AGTTTCTGAA CAAGACCAAC 
AAGAGCGACT ATGTGAGCCG CGACTTTGTC ATCCAGTCGA TACAGGACGA CTACGAGCTG 
ACGGTCAAGA TGCTGCACTG TCCCAGTTGG CCAGAGATGT CCAATCCCAA CAGCATCTAC 
GACTTCATCG TCGATGTGCA CGAGCGCTGC AATGACTATC GCAATGGACC CATCGTCATT 
GTGGATAGAT ATGGTGGCGC CCAGGCGTGC ACCTTCTGTG CCATCTCCTC GCTGGCCATC 
GAGATGGAGT ACTGCAGCAC GGCGAATGTG TACCAGTACG CGAAGCTGTA CCACAACAAG 
CGACCCGGGG TGTGGACATC CAGCGAGGAC ATCCGCGTCA TCTACAACAT ACTTTCATTT 
TTGCCTGGCA ATTTGAACCT GCTGAAGCGC ACGGCGCTTC GGACTGAATT CGAGGATGTG 
ACAACGGCCA CGCCGGATCT CTATAGCAAA ATATGCAGCA ATGGTAATGT TCCACAGCAT 
GTCATACTGC AGCAGCAGCA GCTGCACATG CTGCAGTTGC AGCAGCAACA CCTAGAAACA 
CAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAGCAGCAGC AGCAACAACA GACAGCACTC 
AATGAGACAG TCAGCACACC AAGCACCGAT ACTAATCCAA GCCTCTTGCC CATTCTGTCA 
TTGCTACCGC CCACAGTCGC TCCTCTATCC TCCAGTTCAT CCACCACACC ACCACCACCA 
AGTACTCCAA CACCACAGCC CCCACAAACC ATCCAACTGT CATCGCATTC GCCATCCGAC 
CTGTCCCACC AGATCTCATC CACAGTCGCC AATGCAGGAT CACCAGCAAC TGCAAGTGCA 
GGCGCAACAC CAACGACTCC AATGACACCG ACAGTCCCAC CCACAATACC TACAATACCA 
TCACTCGCAT CCCAGAACAG TCTAACCCTT ACTAATGCCA ATTTCCATAC TGTTACTAAT 
AACGCTGCTG ATCTGATGGA ACACCAACAA CAACAAATGC TGGCCCTGAT GCAACAACAA 
ACGCAACTGC AACAACAATA CAACACGCAC CCGCAACAAC ATCACAACAA CGTCGGTGAC 
CTGCTGATGA ACAATGCGGA CAACTCACCA ACCGCTTCAC CAACGATCAC CAACAACAAT 
CATATAACCA ACAACAACGT CACAAGCGCA GCAGCGACAG ATGCTCAAAA CCTAGATATT 
GTAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ptp99A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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