E(spl)m8-HLH cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	E(spl)m8-HLH
Entrez Gene ID	43161
Full Name	Enhancer of split m8, helix-loop-helix
Synonyms	CG8365,Dmel\CG8365,E(Spl),E(spl),E(spl) m8,E(spl)-C,E(spl)-m8,E(spl)M8,E(spl)bHLH,E(spl)m8,En(spl),En(spl)-C,Espl,HLHm8,Hes,M8,Su(H)[[m8]],anon-EST:Liang-1.71,bHLHb36,clone 1.71,e(spl),l(3)96Fd,m8
General protein information	Preferred Names Enhancer of split m8, helix-loop-helix Names CG8365 gene product from transcript CG8365-RA CG8365-PA E(spl)m8 E(spl)m8-HLH-PA Enhancer-of-split enhancer of split enhancer of split m8 enhancer of split m8, helix-loop-helix enhancer-of-split-m8
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96F10-96F10\|3-89.1 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079789.3	NP_524513.1	enhancer of split m8, helix-loop-helix

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	E(spl)m5-HLH	NP_524511.1
Drosophila melanogaster (fruit fly)	E(spl)m8-HLH	NP_524513.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The conserved MAPK site in E(spl)-M8, an effector of Drosophila notch signaling, controls repressor activity during eye development.
Title: The Conserved MAPK Site in E(spl)-M8, an Effector of Drosophila Notch Signaling, Controls Repressor Activity during Eye Development.

Studies identify PP2A-Wdb as a participant in Notch signaling, and suggest that M8 activity is controlled by phosphorylation and dephosphorylation.
Title: The Ser/Thr phosphatase PP2A regulatory subunit widerborst inhibits notch signaling.

Based on these data, we propose a model in which Hes genes are responsible for co-ordinating the Notch response of a wide spectrum of other targets, explaining the critical functions these key regulators play in many developmental and disease contexts.
Title: Transcriptional dynamics elicited by a short pulse of notch activation involves feed-forward regulation by E(spl)/Hes genes.

Da transactivation domains play essential roles in neurogenesis and in E(spl)-mediated repression.
Title: Essential roles of Da transactivation domains in neurogenesis and in E(spl)-mediated repression.

E(spl) and Dpn act together to maintain the neuroblasts in a self-renewing state, a process in which they are assisted by Notch, which sustains expression of the E(spl) subset
Title: bHLH-O proteins are crucial for Drosophila neuroblast self-renewal and mediate Notch-induced overproliferation.

The following E(spl)m8-HLH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E(spl)m8-HLH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06174	NM_079789.3 Latest version!	Drosophila melanogaster enhancer of split m8, helix-loop-helix (E(spl)m8-HLH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

E(spl)m8-HLH ( NM_079789.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524513.1 Drosophila melanogaster enhancer of split m8, helix-loop-helix (E(spl)m8-HLH), mRNA.

Price & Availability↑

CloneID	OFa06174
Clone ID Related Accession (Same CDS sequence)	NM_079789.3
Accession Version	NM_079789.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of split m8, helix-loop-helix
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079789.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAATACA CCACCAAGAC CCAGATCTAC CAGAAGGTGA AGAAGCCAAT GCTGGAGCGC 
CAGCGACGTG CCCGCATGAA CAAGTGCCTG GACAACCTGA AAACACTTGT CGCCGAGCTA 
CGAGGTGATG ATGGCATCCT GCGCATGGAC AAGGCCGAGA TGCTCGAGTC AGCGGTGATC 
TTCATGCGCC AGCAAAAGAC ACCAAAGAAG GTGGCTCAGG AAGAACAATC CCTCCCCCTG 
GACAGCTTTA AGAATGGCTA CATGAATGCC GTCAACGAGG TGTCACGTGT CATGGCCTCC 
ACACCTGGCA TGAGCGTCGA CCTGGGCAAA TCGGTGATGA CTCACCTGGG ACGCGTCTAC 
AAGAACTTGC AGCAATTCCA CGAAGCACAG TCCGCCGCCG ACTTCATCCA GAACTCGATG 
GACTGCTCCT CAATGGACAA GGCTCCGCTC AGCCCCGCCT CCTCCGGATA TCACAGCGAC 
TGCGACAGCC CCGCCCCCTC GCCACAGCCC ATGCAGCAGC CCTTGTGGCG CCCCTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524513.1
CDS	85..624
Translation	MEYTTKTQIYQKVKKPMLERQRRARMNKCLDNLKTLVAELRGDDGILRMDKAEMLESAVIFMRQQKTPKKVAQEEQSLPLDSFKNGYMNAVNEVSRVMASTPGMSVDLGKSVMTHLGRVYKNLQQFHEAQSAADFIQNSMDCSSMDKAPLSPASSGYHSDCDSPAPSPQPMQQPLWRPW

Target ORF information:

RefSeq Version	NM_079789.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of split m8, helix-loop-helix (E(spl)m8-HLH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079789.3

ORF Insert Sequence:

ATGGAATACA CCACCAAGAC CCAGATCTAC CAGAAGGTGA AGAAGCCAAT GCTGGAGCGC 
CAGCGACGTG CCCGCATGAA CAAGTGCCTG GACAACCTGA AAACACTTGT CGCCGAGCTA 
CGAGGTGATG ATGGCATCCT GCGCATGGAC AAGGCCGAGA TGCTCGAGTC AGCGGTGATC 
TTCATGCGCC AGCAAAAGAC ACCAAAGAAG GTGGCTCAGG AAGAACAATC CCTCCCCCTG 
GACAGCTTTA AGAATGGCTA CATGAATGCC GTCAACGAGG TGTCACGTGT CATGGCCTCC 
ACACCTGGCA TGAGCGTCGA CCTGGGCAAA TCGGTGATGA CTCACCTGGG ACGCGTCTAC 
AAGAACTTGC AGCAATTCCA CGAAGCACAG TCCGCCGCCG ACTTCATCCA GAACTCGATG 
GACTGCTCCT CAATGGACAA GGCTCCGCTC AGCCCCGCCT CCTCCGGATA TCACAGCGAC 
TGCGACAGCC CCGCCCCCTC GCCACAGCCC ATGCAGCAGC CCTTGTGGCG CCCCTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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E(spl)m8-HLH cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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