ana1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ana1
Entrez Gene ID	42927
Full Name	anastral spindle 1
Synonyms	Ana 1,Ana-1,Ana1,CG6631,Dmel\CG6631,mecB
General protein information	Preferred Names anastral spindle 1 Names CG6631 gene product from transcript CG6631-RC CG6631-PA CG6631-PC ana1-PA ana1-PC anastral spindle phenotype 1 mechanosensory B mechanosensory mutant B
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96A6-96A6

mRNA and Protein(s)

mRNA	Protein	Name
NM_170148.3	NP_733008.2	anastral spindle 1, isoform C
NM_143011.3	NP_651268.1	anastral spindle 1, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ana1 mutants lack detectable centrosomes in vivo, that Ana1 is irreversibly incorporated into centrioles during their assembly and appears to play a more important role in maintaining Asl at centrioles than in initially recruiting Asl to centrioles...the N-terminal region of Ana1 (amino acids 1-935) is required to target Ana1 to centrioles through a direct interaction with Cep135, whereas the C-terminal region of Ana1.
Title: Drosophila Ana1 is required for centrosome assembly and centriole elongation.

The following ana1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ana1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05968	NM_170148.3 Latest version!	Drosophila melanogaster anastral spindle 1 (ana1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa05968	NM_143011.3 Latest version!	Drosophila melanogaster anastral spindle 1 (ana1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ana1 ( NM_170148.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733008.2 Drosophila melanogaster anastral spindle 1 (ana1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa05968
Clone ID Related Accession (Same CDS sequence)	NM_170148.3 , NM_143011.3
Accession Version	NM_170148.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	anastral spindle 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170148.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTAACAGT AAATGGAAAG TTCGGGGCCA ACCTGAGGAG GACCATAGGA 
CCACTGGACG ATCCAGAGCA AGTACGCAAA GATCTCGAAA GTGAGCGTCG CATAACCCGT 
TTGAAGCAGG TGCGGGAGAA GTCAAACCAC CTGGCACGCC AGATACGCGA GGATGTGGCT 
GCCGAAAAGA GGCGTCAAAT CCACAAATTA GAGCAGGTCA AGCAAAGGGA GCTAAATGCG 
TGGCGTGAGC ATGTTTTGGT CAAAAAGCAC CAGGACTACC GTTCGGCCAT TTTCCAGGTG 
GGCGCCGCCC ATCGCGCCGC AAAGGAGGAG AACGAACGCA TCGAGCAGCA GAGGCAAGAG 
AGAATCGACA AAATCCGAAG GTGTCGCAAG CAGGCCTTGA AAAGATCGGC TAAAGCCAGC 
GTTGAGTTGC GGACCACAAA CACAATGAAT TTAAATGCGG AGGGACGTGC CTCGGCGGGA 
ACACAAACGC CCATAGTGGA GGACAAAGAA AACCGAATGG AGAGCGGGTG TAACAAACCC 
TGTTGCAAGG GCAAGCGCAA AAGGAAAACC AGCTGTGGAT GTGCCAGCAC CGATGCAGAG 
GATGAGAAGG AGGATGACGC ATCGTCCGAT GACGACTCAT CCATCCTGAT CATCGAGAGT 
CCGAGCAGCA ACCGGCGTCT CCAAAAAACT ACCCCCGTAA TCCTAGACGT AGAAATCGAG 
GAAACCATTT CTGAAACCGA CAAGAATCCC GAGGGTATGG ATATAAATGA TAGGTTCATG 
CAAACCAACC GGAAATTCAG CCATGTCGTG CGACCCAGTG ATGATGAACC CCAACGCCGG 
CCGAGATTCA CTCAGATCAG CGATTTGGTA AGAAAAACCG AGACCACCGT ACGAGCTGGG 
CCAAGTCAAC GAAGGGAAGC AGATCCTACT CCACCAGTAT CTGCCCCGCC TAGTCCCACC 
AAATCCCCGC CAAGATCGCC CAGAAAATGT GTAGAGCGTG AGGAGCCCGT TCCTGCTCCA 
GCACCCAGAA AATCACCCAC TAAAACAGCT AAAGGATCGC CGAAGAAAAC TGTGACGGCA 
CCACGTAACC AGCCATCAGT CAAAAGAACG GGTTTAAAAT TAAATCCAGC TCCTGCCAAG 
GTGATAGATG CGGGAATACA CAGAGGTCAG AAGGCCAAAG CTCCAGCTAC GCTGCCCAAA 
GTAACAGCTA TACAGAAACC AGCAGTGGAA CCTCCTCGAA ACCTTCCACC GATTCCAGAA 
CAGGCTATGC CAGCTGTGCC AAATCCGTCG ATGACCCATT GTTATCCCAT GCCGCAGAAT 
CAACCATACA TGCATCCGTA TGCTCAACTT CCGATGCAAC CATATGCGAT GCCATATTCA 
ATGCCATTTC CCGTGCAAGC CCAGTTTCCA CAACCTCATC CGATGTATGC ACCGCAGCAA 
ATGATGCCGA ATCAGCCAGC TGTACTCGGT CCCGCTATTA CTGCTCCACC GAGCACTGCT 
ACGCAATCCA CTGTGTCCAC CACCACAATT ACCATGTCCT CGCGACAGGA CCCAAGGACG 
ACTCAATGTG GGCGTGTTCA GTTCTATGAT CATAGCAATA AGTACCATAG AACGTACGAA 
GCACCAACAC AGTCGGTGCA GTGCAATGAG AAGGATGCCA CCCAATTGAC CGCTATGGAT 
CATGCTCGCA TCGAGAACCA GCTGAGGGAT CTGCGGGAAC AGGAACTGGA CAAACTGAGG 
AAAATTAGTG ACGATCGTGG TCAAAAGGCT CTTGAACGCG AGCAAGTGCG TCGGGATTGT 
GCTGAATTAA CGGAAAAGCT AGATGCTCTA ACGCAACAGC AACCGCAGCT CTTGCCAACC 
GATGCTAACA TTATACCTAG CCATCGGTAC GCGGATGCAG CTGCCAGGCG AGAGCAAAAG 
ATGAACGATG CAATGGAAGA AATGCTGCTT CGACCAGCCA TTATCACCTG CCCGGAAGTG 
CGCACCAAAC CTACTCCTCC AAACTTATCG CGCAGCAAAT CCAAAGCTTC TGTGGCAGTT 
AATTTGGGCG AACCTCCTGT TCAAATCGGC CGGGATAATC TTGGCAGCTC GGAGAGCTGC 
AGCTCCATTC TTCTCGACTA CGTTAATGAT CAAAGCAAGC AACTCAAGTC GGACCTGCAG 
GCCGAGCAGT CAAACTCACT GAAATCGATG AGGCTAAAGA GTTTGCTTGA GCGCATTGAG 
AAAATTCGAG TCCAGCTGTT GGAGGAACTG AAGGCGGGTG AGTCAGGTGC ATCAAAGGGT 
GACAATGCAC AGGAATTGAA TAATATACGT CAGGAGCGAG CTGATATTCT ATCCGAGCGA 
ACGAGAACTT TAAACGAACG AGAATCTGAT TTGCAACAGA AGGAGGCTAT TCTCGAGCAG 
CGTCTAAGAA AGTTCTACAA GGAGACAAAA AACGCAAAAT CCAGTGAGAG TGATAAGCCA 
AAATCAAACG CAAAAGAAGA TGGGCCAGTT GAGATCATTA TTAAGGTGAG GAGTGACGGT 
ACTGTTAAGC AATATGTACC GAGAACAAAA TCGAAAGCCA AAACCAAGCC GTCGGCTATC 
GACAATGAAA AGGATGTGCC CCTGGGCTCA ACTGACAGCA CACCAAGAGA CGATGAGCCA 
AAGCCGACGG AACAGGATCA CGGAAAGCGA CCATTCTTAC AGGATCAGCG TCAGATATCC 
ATTGATTCCA ACTCCACTGC CTATCGCAGT CTGCCGCCAG TGAGCTATAA AAATATGACC 
ACGGCCGCTC CCCCTTCTGC TCCATTACAT CCCATGGTGG TTCAATATAT AAATCGTCTG 
CTAGGAATGA CACGCCAAAA TATCGATGAG ATGGGTGTGA GTTCCTCGTG TGTGACCACA 
CCATCAACGT CGGTGATCAA CTCATTGAGA AACGTAACCC CTTGTCCCGA GCCTGAAGCG 
GGAAGAGCTG AAAGGGATCA GGATGAAAAT ACGGAGATGA ACGAGCATCG CATGGAGCGG 
GTGCAAACCT TTATTGCCGA CAATCGCAGC TTAATCAATG ACTTGGAAGA ATCAATACGT 
TGCCAGCAGC AGCTGCAAAG GGAGCAACAA TCGCTGGATG CAGAGAAAAG TATGCGCGCA 
TTCGATCAAA TATGGAACAA GCGTTTGGCC AAGAATCCTG AAGATTGTCA GCAAACCAAC 
AAAGAGAAAG AGCAAACAAG AGAAAAGATG CCAAGGGAAA GGCCGACTCC ACAAGGAGAT 
AGGAAAAATG CAGCACCTCC ACAGGAAAGC GGCAAAATTC GTAGAACTCA AGAAGGGGAC 
CGGCAGCAGC AAACAAACGG TGCAGTGCAA CAAAAAAGGG AGCAAAAGAC TGTAAAACAA 
GGTTCAGGCG CCGATAAAAG CACAAACCGC TTTGCCCCCG AAAAATCGAA AGCACTATCC 
ACCAGTGAGG AGTCATCTGC CCGAAACATG GAACGCTATG CACAGCTCAC GGAGAACTGC 
ACCCAGCGGA TCGCCGAACT TACAGAGCTG ATCACGAAAG TGCGCGAGGA GAAGCAGCGA 
CTGGTGGAGG TCACCCTCAC ATCGAACAGC GATGGCGAGC GGCAGTCCAC TGAATATCTT 
GAGCTTCCAA CAGGACAACA GCAAACCAGA TGCCGGACAA TTTCCGATCG CAGCGATTCT 
CAAACACCTT CGACCTCGGA AGCCTTGCCG TTGCAGAAGC ACAAGCCGAC AGCGGCCAGC 
AGGGATAGTG GCATTGCGGA CTCACGTCCA ATTACGGCAC AGGGTCAGGT TTGTATCGAT 
GTGGAACCCA TCTCGCTTGG GTCATCAACG CAGAATAATA CAGCACGCGG ACGAGTAAGG 
GCACCTCCGG CCACGATTCG TCGGTATAGT CCTCAACTAA ACGCCGAGGA TCTGGCGCAC 
GAGCTGTCGA CCATTACAGA AGTGGAAACG CCAGGACAAT CACATATTGT GGCCGCCACA 
CCGGTGCCCA AGCCATTCCC TAGTTTCGAT CAGTATGCCA AGGAACTGCA CTTAGATCTG 
TCGCGGTTGG ATGCTGATCA GAGCCAACGG CTGCAGGGCG AATTTAATGA CTTAATACTC 
GCGATCAGGC AACGCAATCA AGGAACTGAC TACCGTGAGT TTCCTAGCAT TAATGCCTAT 
CTTCATAACA TGACTAGCAC CCGAATCCAT GTGGAGATAG GTCAGGATGC AGATCAGGCC 
ACTCTGTCAC CGGGTGAACT GATGCGACAG CTGCGCGTGA TCACTCATAG TATCCAAGAC 
TTTCCCAAGC GCAGGGAATA CTTTGAAAAA CTTATGGCCA AGCAACCACC CGATCAGAGG 
GATCTAATAG ACAGTGCTAG CTTAGAGAAT GATTCCACGG ATTCATTTAA CGTAGAGGAA 
GAACTCCGCC AGCGGAAAAT TCTAAAGAAC TCATTTCGTC GAGGAAACGC AACTGAGACC 
ACATTGACGG CTCAGGAGGT GGCTAGCAGT ACGCGGCGGG AAAGCGTCGC CCCAGTGACC 
AACGATCAAC CCAACGAGAG TGGCATAGAT CCCCTCTCCG GCAGCAACTT TTCCAGCGAC 
GCGGATCAGA GAAGTCCGTG CTGGCATGCC ACAATGCATG AGAGGCAACA GCAGGTGGAT 
GAGTTGTGCA GCAGTACCAC AGCCTCGTCG CCAGAACGGC GGCCACGAAA GTCTCGGCTT 
CGTGGAGGCC ACGAGCACAA CAATAGGTCT AAGGACGATT CCACTGTGCA GGATGCAAGC 
CAGATAGGAA GATCCCTTAA TCTGAGGGAA TTCCTTACGA GAGAGCTCCT CAAACATCGA 
GTGCACGACG ACGTTGCCAG TGAGAGTTCA GATGAGTCCT TAAGGGGGCA CTTTCTGAAG 
TCGGTCCTAC ATTCGCTCTC GCCCAGCAAC AACACGCAAA CGCCAGGAGT GGGTGTAAGC 
CATGCCACCG GAGCAACAAA CGACAGACAA AAGACATCCA CACCAGTGGG ATCGTTCCTC 
TCCATTCCAG AGAAGGCCGG GTCCATGAGT AACACCGGCT CTCAACTGTT CTCCGGGGAG 
AGTCGCATCT CCCTGGTTAA CTATCCAGAT GGAACTCCAC CCATTCCTTT TGAACAACAA 
AGCAAACTTA GTACAAATCA AACTCGCCAA TCCTCAGGTC AAATAGGATC AGGTGTCAGG 
ACAACGCATC GAAAGTCGCC CCGAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733008.2
CDS	211..5400
Translation	MALQLTVNGKFGANLRRTIGPLDDPEQVRKDLESERRITRLKQVREKSNHLARQIREDVAAEKRRQIHKLEQVKQRELNAWREHVLVKKHQDYRSAIFQVGAAHRAAKEENERIEQQRQERIDKIRRCRKQALKRSAKASVELRTTNTMNLNAEGRASAGTQTPIVEDKENRMESGCNKPCCKGKRKRKTSCGCASTDAEDEKEDDASSDDDSSILIIESPSSNRRLQKTTPVILDVEIEETISETDKNPEGMDINDRFMQTNRKFSHVVRPSDDEPQRRPRFTQISDLVRKTETTVRAGPSQRREADPTPPVSAPPSPTKSPPRSPRKCVEREEPVPAPAPRKSPTKTAKGSPKKTVTAPRNQPSVKRTGLKLNPAPAKVIDAGIHRGQKAKAPATLPKVTAIQKPAVEPPRNLPPIPEQAMPAVPNPSMTHCYPMPQNQPYMHPYAQLPMQPYAMPYSMPFPVQAQFPQPHPMYAPQQMMPNQPAVLGPAITAPPSTATQSTVSTTTITMSSRQDPRTTQCGRVQFYDHSNKYHRTYEAPTQSVQCNEKDATQLTAMDHARIENQLRDLREQELDKLRKISDDRGQKALEREQVRRDCAELTEKLDALTQQQPQLLPTDANIIPSHRYADAAARREQKMNDAMEEMLLRPAIITCPEVRTKPTPPNLSRSKSKASVAVNLGEPPVQIGRDNLGSSESCSSILLDYVNDQSKQLKSDLQAEQSNSLKSMRLKSLLERIEKIRVQLLEELKAGESGASKGDNAQELNNIRQERADILSERTRTLNERESDLQQKEAILEQRLRKFYKETKNAKSSESDKPKSNAKEDGPVEIIIKVRSDGTVKQYVPRTKSKAKTKPSAIDNEKDVPLGSTDSTPRDDEPKPTEQDHGKRPFLQDQRQISIDSNSTAYRSLPPVSYKNMTTAAPPSAPLHPMVVQYINRLLGMTRQNIDEMGVSSSCVTTPSTSVINSLRNVTPCPEPEAGRAERDQDENTEMNEHRMERVQTFIADNRSLINDLEESIRCQQQLQREQQSLDAEKSMRAFDQIWNKRLAKNPEDCQQTNKEKEQTREKMPRERPTPQGDRKNAAPPQESGKIRRTQEGDRQQQTNGAVQQKREQKTVKQGSGADKSTNRFAPEKSKALSTSEESSARNMERYAQLTENCTQRIAELTELITKVREEKQRLVEVTLTSNSDGERQSTEYLELPTGQQQTRCRTISDRSDSQTPSTSEALPLQKHKPTAASRDSGIADSRPITAQGQVCIDVEPISLGSSTQNNTARGRVRAPPATIRRYSPQLNAEDLAHELSTITEVETPGQSHIVAATPVPKPFPSFDQYAKELHLDLSRLDADQSQRLQGEFNDLILAIRQRNQGTDYREFPSINAYLHNMTSTRIHVEIGQDADQATLSPGELMRQLRVITHSIQDFPKRREYFEKLMAKQPPDQRDLIDSASLENDSTDSFNVEEELRQRKILKNSFRRGNATETTLTAQEVASSTRRESVAPVTNDQPNESGIDPLSGSNFSSDADQRSPCWHATMHERQQQVDELCSSTTASSPERRPRKSRLRGGHEHNNRSKDDSTVQDASQIGRSLNLREFLTRELLKHRVHDDVASESSDESLRGHFLKSVLHSLSPSNNTQTPGVGVSHATGATNDRQKTSTPVGSFLSIPEKAGSMSNTGSQLFSGESRISLVNYPDGTPPIPFEQQSKLSTNQTRQSSGQIGSGVRTTHRKSPRK

Target ORF information:

RefSeq Version	NM_170148.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster anastral spindle 1 (ana1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170148.3

ORF Insert Sequence:

ATGGCTCTGC AGCTAACAGT AAATGGAAAG TTCGGGGCCA ACCTGAGGAG GACCATAGGA 
CCACTGGACG ATCCAGAGCA AGTACGCAAA GATCTCGAAA GTGAGCGTCG CATAACCCGT 
TTGAAGCAGG TGCGGGAGAA GTCAAACCAC CTGGCACGCC AGATACGCGA GGATGTGGCT 
GCCGAAAAGA GGCGTCAAAT CCACAAATTA GAGCAGGTCA AGCAAAGGGA GCTAAATGCG 
TGGCGTGAGC ATGTTTTGGT CAAAAAGCAC CAGGACTACC GTTCGGCCAT TTTCCAGGTG 
GGCGCCGCCC ATCGCGCCGC AAAGGAGGAG AACGAACGCA TCGAGCAGCA GAGGCAAGAG 
AGAATCGACA AAATCCGAAG GTGTCGCAAG CAGGCCTTGA AAAGATCGGC TAAAGCCAGC 
GTTGAGTTGC GGACCACAAA CACAATGAAT TTAAATGCGG AGGGACGTGC CTCGGCGGGA 
ACACAAACGC CCATAGTGGA GGACAAAGAA AACCGAATGG AGAGCGGGTG TAACAAACCC 
TGTTGCAAGG GCAAGCGCAA AAGGAAAACC AGCTGTGGAT GTGCCAGCAC CGATGCAGAG 
GATGAGAAGG AGGATGACGC ATCGTCCGAT GACGACTCAT CCATCCTGAT CATCGAGAGT 
CCGAGCAGCA ACCGGCGTCT CCAAAAAACT ACCCCCGTAA TCCTAGACGT AGAAATCGAG 
GAAACCATTT CTGAAACCGA CAAGAATCCC GAGGGTATGG ATATAAATGA TAGGTTCATG 
CAAACCAACC GGAAATTCAG CCATGTCGTG CGACCCAGTG ATGATGAACC CCAACGCCGG 
CCGAGATTCA CTCAGATCAG CGATTTGGTA AGAAAAACCG AGACCACCGT ACGAGCTGGG 
CCAAGTCAAC GAAGGGAAGC AGATCCTACT CCACCAGTAT CTGCCCCGCC TAGTCCCACC 
AAATCCCCGC CAAGATCGCC CAGAAAATGT GTAGAGCGTG AGGAGCCCGT TCCTGCTCCA 
GCACCCAGAA AATCACCCAC TAAAACAGCT AAAGGATCGC CGAAGAAAAC TGTGACGGCA 
CCACGTAACC AGCCATCAGT CAAAAGAACG GGTTTAAAAT TAAATCCAGC TCCTGCCAAG 
GTGATAGATG CGGGAATACA CAGAGGTCAG AAGGCCAAAG CTCCAGCTAC GCTGCCCAAA 
GTAACAGCTA TACAGAAACC AGCAGTGGAA CCTCCTCGAA ACCTTCCACC GATTCCAGAA 
CAGGCTATGC CAGCTGTGCC AAATCCGTCG ATGACCCATT GTTATCCCAT GCCGCAGAAT 
CAACCATACA TGCATCCGTA TGCTCAACTT CCGATGCAAC CATATGCGAT GCCATATTCA 
ATGCCATTTC CCGTGCAAGC CCAGTTTCCA CAACCTCATC CGATGTATGC ACCGCAGCAA 
ATGATGCCGA ATCAGCCAGC TGTACTCGGT CCCGCTATTA CTGCTCCACC GAGCACTGCT 
ACGCAATCCA CTGTGTCCAC CACCACAATT ACCATGTCCT CGCGACAGGA CCCAAGGACG 
ACTCAATGTG GGCGTGTTCA GTTCTATGAT CATAGCAATA AGTACCATAG AACGTACGAA 
GCACCAACAC AGTCGGTGCA GTGCAATGAG AAGGATGCCA CCCAATTGAC CGCTATGGAT 
CATGCTCGCA TCGAGAACCA GCTGAGGGAT CTGCGGGAAC AGGAACTGGA CAAACTGAGG 
AAAATTAGTG ACGATCGTGG TCAAAAGGCT CTTGAACGCG AGCAAGTGCG TCGGGATTGT 
GCTGAATTAA CGGAAAAGCT AGATGCTCTA ACGCAACAGC AACCGCAGCT CTTGCCAACC 
GATGCTAACA TTATACCTAG CCATCGGTAC GCGGATGCAG CTGCCAGGCG AGAGCAAAAG 
ATGAACGATG CAATGGAAGA AATGCTGCTT CGACCAGCCA TTATCACCTG CCCGGAAGTG 
CGCACCAAAC CTACTCCTCC AAACTTATCG CGCAGCAAAT CCAAAGCTTC TGTGGCAGTT 
AATTTGGGCG AACCTCCTGT TCAAATCGGC CGGGATAATC TTGGCAGCTC GGAGAGCTGC 
AGCTCCATTC TTCTCGACTA CGTTAATGAT CAAAGCAAGC AACTCAAGTC GGACCTGCAG 
GCCGAGCAGT CAAACTCACT GAAATCGATG AGGCTAAAGA GTTTGCTTGA GCGCATTGAG 
AAAATTCGAG TCCAGCTGTT GGAGGAACTG AAGGCGGGTG AGTCAGGTGC ATCAAAGGGT 
GACAATGCAC AGGAATTGAA TAATATACGT CAGGAGCGAG CTGATATTCT ATCCGAGCGA 
ACGAGAACTT TAAACGAACG AGAATCTGAT TTGCAACAGA AGGAGGCTAT TCTCGAGCAG 
CGTCTAAGAA AGTTCTACAA GGAGACAAAA AACGCAAAAT CCAGTGAGAG TGATAAGCCA 
AAATCAAACG CAAAAGAAGA TGGGCCAGTT GAGATCATTA TTAAGGTGAG GAGTGACGGT 
ACTGTTAAGC AATATGTACC GAGAACAAAA TCGAAAGCCA AAACCAAGCC GTCGGCTATC 
GACAATGAAA AGGATGTGCC CCTGGGCTCA ACTGACAGCA CACCAAGAGA CGATGAGCCA 
AAGCCGACGG AACAGGATCA CGGAAAGCGA CCATTCTTAC AGGATCAGCG TCAGATATCC 
ATTGATTCCA ACTCCACTGC CTATCGCAGT CTGCCGCCAG TGAGCTATAA AAATATGACC 
ACGGCCGCTC CCCCTTCTGC TCCATTACAT CCCATGGTGG TTCAATATAT AAATCGTCTG 
CTAGGAATGA CACGCCAAAA TATCGATGAG ATGGGTGTGA GTTCCTCGTG TGTGACCACA 
CCATCAACGT CGGTGATCAA CTCATTGAGA AACGTAACCC CTTGTCCCGA GCCTGAAGCG 
GGAAGAGCTG AAAGGGATCA GGATGAAAAT ACGGAGATGA ACGAGCATCG CATGGAGCGG 
GTGCAAACCT TTATTGCCGA CAATCGCAGC TTAATCAATG ACTTGGAAGA ATCAATACGT 
TGCCAGCAGC AGCTGCAAAG GGAGCAACAA TCGCTGGATG CAGAGAAAAG TATGCGCGCA 
TTCGATCAAA TATGGAACAA GCGTTTGGCC AAGAATCCTG AAGATTGTCA GCAAACCAAC 
AAAGAGAAAG AGCAAACAAG AGAAAAGATG CCAAGGGAAA GGCCGACTCC ACAAGGAGAT 
AGGAAAAATG CAGCACCTCC ACAGGAAAGC GGCAAAATTC GTAGAACTCA AGAAGGGGAC 
CGGCAGCAGC AAACAAACGG TGCAGTGCAA CAAAAAAGGG AGCAAAAGAC TGTAAAACAA 
GGTTCAGGCG CCGATAAAAG CACAAACCGC TTTGCCCCCG AAAAATCGAA AGCACTATCC 
ACCAGTGAGG AGTCATCTGC CCGAAACATG GAACGCTATG CACAGCTCAC GGAGAACTGC 
ACCCAGCGGA TCGCCGAACT TACAGAGCTG ATCACGAAAG TGCGCGAGGA GAAGCAGCGA 
CTGGTGGAGG TCACCCTCAC ATCGAACAGC GATGGCGAGC GGCAGTCCAC TGAATATCTT 
GAGCTTCCAA CAGGACAACA GCAAACCAGA TGCCGGACAA TTTCCGATCG CAGCGATTCT 
CAAACACCTT CGACCTCGGA AGCCTTGCCG TTGCAGAAGC ACAAGCCGAC AGCGGCCAGC 
AGGGATAGTG GCATTGCGGA CTCACGTCCA ATTACGGCAC AGGGTCAGGT TTGTATCGAT 
GTGGAACCCA TCTCGCTTGG GTCATCAACG CAGAATAATA CAGCACGCGG ACGAGTAAGG 
GCACCTCCGG CCACGATTCG TCGGTATAGT CCTCAACTAA ACGCCGAGGA TCTGGCGCAC 
GAGCTGTCGA CCATTACAGA AGTGGAAACG CCAGGACAAT CACATATTGT GGCCGCCACA 
CCGGTGCCCA AGCCATTCCC TAGTTTCGAT CAGTATGCCA AGGAACTGCA CTTAGATCTG 
TCGCGGTTGG ATGCTGATCA GAGCCAACGG CTGCAGGGCG AATTTAATGA CTTAATACTC 
GCGATCAGGC AACGCAATCA AGGAACTGAC TACCGTGAGT TTCCTAGCAT TAATGCCTAT 
CTTCATAACA TGACTAGCAC CCGAATCCAT GTGGAGATAG GTCAGGATGC AGATCAGGCC 
ACTCTGTCAC CGGGTGAACT GATGCGACAG CTGCGCGTGA TCACTCATAG TATCCAAGAC 
TTTCCCAAGC GCAGGGAATA CTTTGAAAAA CTTATGGCCA AGCAACCACC CGATCAGAGG 
GATCTAATAG ACAGTGCTAG CTTAGAGAAT GATTCCACGG ATTCATTTAA CGTAGAGGAA 
GAACTCCGCC AGCGGAAAAT TCTAAAGAAC TCATTTCGTC GAGGAAACGC AACTGAGACC 
ACATTGACGG CTCAGGAGGT GGCTAGCAGT ACGCGGCGGG AAAGCGTCGC CCCAGTGACC 
AACGATCAAC CCAACGAGAG TGGCATAGAT CCCCTCTCCG GCAGCAACTT TTCCAGCGAC 
GCGGATCAGA GAAGTCCGTG CTGGCATGCC ACAATGCATG AGAGGCAACA GCAGGTGGAT 
GAGTTGTGCA GCAGTACCAC AGCCTCGTCG CCAGAACGGC GGCCACGAAA GTCTCGGCTT 
CGTGGAGGCC ACGAGCACAA CAATAGGTCT AAGGACGATT CCACTGTGCA GGATGCAAGC 
CAGATAGGAA GATCCCTTAA TCTGAGGGAA TTCCTTACGA GAGAGCTCCT CAAACATCGA 
GTGCACGACG ACGTTGCCAG TGAGAGTTCA GATGAGTCCT TAAGGGGGCA CTTTCTGAAG 
TCGGTCCTAC ATTCGCTCTC GCCCAGCAAC AACACGCAAA CGCCAGGAGT GGGTGTAAGC 
CATGCCACCG GAGCAACAAA CGACAGACAA AAGACATCCA CACCAGTGGG ATCGTTCCTC 
TCCATTCCAG AGAAGGCCGG GTCCATGAGT AACACCGGCT CTCAACTGTT CTCCGGGGAG 
AGTCGCATCT CCCTGGTTAA CTATCCAGAT GGAACTCCAC CCATTCCTTT TGAACAACAA 
AGCAAACTTA GTACAAATCA AACTCGCCAA TCCTCAGGTC AAATAGGATC AGGTGTCAGG 
ACAACGCATC GAAAGTCGCC CCGAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ana1 ( NM_143011.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651268.1 Drosophila melanogaster anastral spindle 1 (ana1), transcript variant A, mRNA.

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CloneID	OFa05968
Clone ID Related Accession (Same CDS sequence)	NM_170148.3 , NM_143011.3
Accession Version	NM_143011.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	anastral spindle 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143011.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTAACAGT AAATGGAAAG TTCGGGGCCA ACCTGAGGAG GACCATAGGA 
CCACTGGACG ATCCAGAGCA AGTACGCAAA GATCTCGAAA GTGAGCGTCG CATAACCCGT 
TTGAAGCAGG TGCGGGAGAA GTCAAACCAC CTGGCACGCC AGATACGCGA GGATGTGGCT 
GCCGAAAAGA GGCGTCAAAT CCACAAATTA GAGCAGGTCA AGCAAAGGGA GCTAAATGCG 
TGGCGTGAGC ATGTTTTGGT CAAAAAGCAC CAGGACTACC GTTCGGCCAT TTTCCAGGTG 
GGCGCCGCCC ATCGCGCCGC AAAGGAGGAG AACGAACGCA TCGAGCAGCA GAGGCAAGAG 
AGAATCGACA AAATCCGAAG GTGTCGCAAG CAGGCCTTGA AAAGATCGGC TAAAGCCAGC 
GTTGAGTTGC GGACCACAAA CACAATGAAT TTAAATGCGG AGGGACGTGC CTCGGCGGGA 
ACACAAACGC CCATAGTGGA GGACAAAGAA AACCGAATGG AGAGCGGGTG TAACAAACCC 
TGTTGCAAGG GCAAGCGCAA AAGGAAAACC AGCTGTGGAT GTGCCAGCAC CGATGCAGAG 
GATGAGAAGG AGGATGACGC ATCGTCCGAT GACGACTCAT CCATCCTGAT CATCGAGAGT 
CCGAGCAGCA ACCGGCGTCT CCAAAAAACT ACCCCCGTAA TCCTAGACGT AGAAATCGAG 
GAAACCATTT CTGAAACCGA CAAGAATCCC GAGGGTATGG ATATAAATGA TAGGTTCATG 
CAAACCAACC GGAAATTCAG CCATGTCGTG CGACCCAGTG ATGATGAACC CCAACGCCGG 
CCGAGATTCA CTCAGATCAG CGATTTGGTA AGAAAAACCG AGACCACCGT ACGAGCTGGG 
CCAAGTCAAC GAAGGGAAGC AGATCCTACT CCACCAGTAT CTGCCCCGCC TAGTCCCACC 
AAATCCCCGC CAAGATCGCC CAGAAAATGT GTAGAGCGTG AGGAGCCCGT TCCTGCTCCA 
GCACCCAGAA AATCACCCAC TAAAACAGCT AAAGGATCGC CGAAGAAAAC TGTGACGGCA 
CCACGTAACC AGCCATCAGT CAAAAGAACG GGTTTAAAAT TAAATCCAGC TCCTGCCAAG 
GTGATAGATG CGGGAATACA CAGAGGTCAG AAGGCCAAAG CTCCAGCTAC GCTGCCCAAA 
GTAACAGCTA TACAGAAACC AGCAGTGGAA CCTCCTCGAA ACCTTCCACC GATTCCAGAA 
CAGGCTATGC CAGCTGTGCC AAATCCGTCG ATGACCCATT GTTATCCCAT GCCGCAGAAT 
CAACCATACA TGCATCCGTA TGCTCAACTT CCGATGCAAC CATATGCGAT GCCATATTCA 
ATGCCATTTC CCGTGCAAGC CCAGTTTCCA CAACCTCATC CGATGTATGC ACCGCAGCAA 
ATGATGCCGA ATCAGCCAGC TGTACTCGGT CCCGCTATTA CTGCTCCACC GAGCACTGCT 
ACGCAATCCA CTGTGTCCAC CACCACAATT ACCATGTCCT CGCGACAGGA CCCAAGGACG 
ACTCAATGTG GGCGTGTTCA GTTCTATGAT CATAGCAATA AGTACCATAG AACGTACGAA 
GCACCAACAC AGTCGGTGCA GTGCAATGAG AAGGATGCCA CCCAATTGAC CGCTATGGAT 
CATGCTCGCA TCGAGAACCA GCTGAGGGAT CTGCGGGAAC AGGAACTGGA CAAACTGAGG 
AAAATTAGTG ACGATCGTGG TCAAAAGGCT CTTGAACGCG AGCAAGTGCG TCGGGATTGT 
GCTGAATTAA CGGAAAAGCT AGATGCTCTA ACGCAACAGC AACCGCAGCT CTTGCCAACC 
GATGCTAACA TTATACCTAG CCATCGGTAC GCGGATGCAG CTGCCAGGCG AGAGCAAAAG 
ATGAACGATG CAATGGAAGA AATGCTGCTT CGACCAGCCA TTATCACCTG CCCGGAAGTG 
CGCACCAAAC CTACTCCTCC AAACTTATCG CGCAGCAAAT CCAAAGCTTC TGTGGCAGTT 
AATTTGGGCG AACCTCCTGT TCAAATCGGC CGGGATAATC TTGGCAGCTC GGAGAGCTGC 
AGCTCCATTC TTCTCGACTA CGTTAATGAT CAAAGCAAGC AACTCAAGTC GGACCTGCAG 
GCCGAGCAGT CAAACTCACT GAAATCGATG AGGCTAAAGA GTTTGCTTGA GCGCATTGAG 
AAAATTCGAG TCCAGCTGTT GGAGGAACTG AAGGCGGGTG AGTCAGGTGC ATCAAAGGGT 
GACAATGCAC AGGAATTGAA TAATATACGT CAGGAGCGAG CTGATATTCT ATCCGAGCGA 
ACGAGAACTT TAAACGAACG AGAATCTGAT TTGCAACAGA AGGAGGCTAT TCTCGAGCAG 
CGTCTAAGAA AGTTCTACAA GGAGACAAAA AACGCAAAAT CCAGTGAGAG TGATAAGCCA 
AAATCAAACG CAAAAGAAGA TGGGCCAGTT GAGATCATTA TTAAGGTGAG GAGTGACGGT 
ACTGTTAAGC AATATGTACC GAGAACAAAA TCGAAAGCCA AAACCAAGCC GTCGGCTATC 
GACAATGAAA AGGATGTGCC CCTGGGCTCA ACTGACAGCA CACCAAGAGA CGATGAGCCA 
AAGCCGACGG AACAGGATCA CGGAAAGCGA CCATTCTTAC AGGATCAGCG TCAGATATCC 
ATTGATTCCA ACTCCACTGC CTATCGCAGT CTGCCGCCAG TGAGCTATAA AAATATGACC 
ACGGCCGCTC CCCCTTCTGC TCCATTACAT CCCATGGTGG TTCAATATAT AAATCGTCTG 
CTAGGAATGA CACGCCAAAA TATCGATGAG ATGGGTGTGA GTTCCTCGTG TGTGACCACA 
CCATCAACGT CGGTGATCAA CTCATTGAGA AACGTAACCC CTTGTCCCGA GCCTGAAGCG 
GGAAGAGCTG AAAGGGATCA GGATGAAAAT ACGGAGATGA ACGAGCATCG CATGGAGCGG 
GTGCAAACCT TTATTGCCGA CAATCGCAGC TTAATCAATG ACTTGGAAGA ATCAATACGT 
TGCCAGCAGC AGCTGCAAAG GGAGCAACAA TCGCTGGATG CAGAGAAAAG TATGCGCGCA 
TTCGATCAAA TATGGAACAA GCGTTTGGCC AAGAATCCTG AAGATTGTCA GCAAACCAAC 
AAAGAGAAAG AGCAAACAAG AGAAAAGATG CCAAGGGAAA GGCCGACTCC ACAAGGAGAT 
AGGAAAAATG CAGCACCTCC ACAGGAAAGC GGCAAAATTC GTAGAACTCA AGAAGGGGAC 
CGGCAGCAGC AAACAAACGG TGCAGTGCAA CAAAAAAGGG AGCAAAAGAC TGTAAAACAA 
GGTTCAGGCG CCGATAAAAG CACAAACCGC TTTGCCCCCG AAAAATCGAA AGCACTATCC 
ACCAGTGAGG AGTCATCTGC CCGAAACATG GAACGCTATG CACAGCTCAC GGAGAACTGC 
ACCCAGCGGA TCGCCGAACT TACAGAGCTG ATCACGAAAG TGCGCGAGGA GAAGCAGCGA 
CTGGTGGAGG TCACCCTCAC ATCGAACAGC GATGGCGAGC GGCAGTCCAC TGAATATCTT 
GAGCTTCCAA CAGGACAACA GCAAACCAGA TGCCGGACAA TTTCCGATCG CAGCGATTCT 
CAAACACCTT CGACCTCGGA AGCCTTGCCG TTGCAGAAGC ACAAGCCGAC AGCGGCCAGC 
AGGGATAGTG GCATTGCGGA CTCACGTCCA ATTACGGCAC AGGGTCAGGT TTGTATCGAT 
GTGGAACCCA TCTCGCTTGG GTCATCAACG CAGAATAATA CAGCACGCGG ACGAGTAAGG 
GCACCTCCGG CCACGATTCG TCGGTATAGT CCTCAACTAA ACGCCGAGGA TCTGGCGCAC 
GAGCTGTCGA CCATTACAGA AGTGGAAACG CCAGGACAAT CACATATTGT GGCCGCCACA 
CCGGTGCCCA AGCCATTCCC TAGTTTCGAT CAGTATGCCA AGGAACTGCA CTTAGATCTG 
TCGCGGTTGG ATGCTGATCA GAGCCAACGG CTGCAGGGCG AATTTAATGA CTTAATACTC 
GCGATCAGGC AACGCAATCA AGGAACTGAC TACCGTGAGT TTCCTAGCAT TAATGCCTAT 
CTTCATAACA TGACTAGCAC CCGAATCCAT GTGGAGATAG GTCAGGATGC AGATCAGGCC 
ACTCTGTCAC CGGGTGAACT GATGCGACAG CTGCGCGTGA TCACTCATAG TATCCAAGAC 
TTTCCCAAGC GCAGGGAATA CTTTGAAAAA CTTATGGCCA AGCAACCACC CGATCAGAGG 
GATCTAATAG ACAGTGCTAG CTTAGAGAAT GATTCCACGG ATTCATTTAA CGTAGAGGAA 
GAACTCCGCC AGCGGAAAAT TCTAAAGAAC TCATTTCGTC GAGGAAACGC AACTGAGACC 
ACATTGACGG CTCAGGAGGT GGCTAGCAGT ACGCGGCGGG AAAGCGTCGC CCCAGTGACC 
AACGATCAAC CCAACGAGAG TGGCATAGAT CCCCTCTCCG GCAGCAACTT TTCCAGCGAC 
GCGGATCAGA GAAGTCCGTG CTGGCATGCC ACAATGCATG AGAGGCAACA GCAGGTGGAT 
GAGTTGTGCA GCAGTACCAC AGCCTCGTCG CCAGAACGGC GGCCACGAAA GTCTCGGCTT 
CGTGGAGGCC ACGAGCACAA CAATAGGTCT AAGGACGATT CCACTGTGCA GGATGCAAGC 
CAGATAGGAA GATCCCTTAA TCTGAGGGAA TTCCTTACGA GAGAGCTCCT CAAACATCGA 
GTGCACGACG ACGTTGCCAG TGAGAGTTCA GATGAGTCCT TAAGGGGGCA CTTTCTGAAG 
TCGGTCCTAC ATTCGCTCTC GCCCAGCAAC AACACGCAAA CGCCAGGAGT GGGTGTAAGC 
CATGCCACCG GAGCAACAAA CGACAGACAA AAGACATCCA CACCAGTGGG ATCGTTCCTC 
TCCATTCCAG AGAAGGCCGG GTCCATGAGT AACACCGGCT CTCAACTGTT CTCCGGGGAG 
AGTCGCATCT CCCTGGTTAA CTATCCAGAT GGAACTCCAC CCATTCCTTT TGAACAACAA 
AGCAAACTTA GTACAAATCA AACTCGCCAA TCCTCAGGTC AAATAGGATC AGGTGTCAGG 
ACAACGCATC GAAAGTCGCC CCGAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651268.1
CDS	127..5316
Translation	MALQLTVNGKFGANLRRTIGPLDDPEQVRKDLESERRITRLKQVREKSNHLARQIREDVAAEKRRQIHKLEQVKQRELNAWREHVLVKKHQDYRSAIFQVGAAHRAAKEENERIEQQRQERIDKIRRCRKQALKRSAKASVELRTTNTMNLNAEGRASAGTQTPIVEDKENRMESGCNKPCCKGKRKRKTSCGCASTDAEDEKEDDASSDDDSSILIIESPSSNRRLQKTTPVILDVEIEETISETDKNPEGMDINDRFMQTNRKFSHVVRPSDDEPQRRPRFTQISDLVRKTETTVRAGPSQRREADPTPPVSAPPSPTKSPPRSPRKCVEREEPVPAPAPRKSPTKTAKGSPKKTVTAPRNQPSVKRTGLKLNPAPAKVIDAGIHRGQKAKAPATLPKVTAIQKPAVEPPRNLPPIPEQAMPAVPNPSMTHCYPMPQNQPYMHPYAQLPMQPYAMPYSMPFPVQAQFPQPHPMYAPQQMMPNQPAVLGPAITAPPSTATQSTVSTTTITMSSRQDPRTTQCGRVQFYDHSNKYHRTYEAPTQSVQCNEKDATQLTAMDHARIENQLRDLREQELDKLRKISDDRGQKALEREQVRRDCAELTEKLDALTQQQPQLLPTDANIIPSHRYADAAARREQKMNDAMEEMLLRPAIITCPEVRTKPTPPNLSRSKSKASVAVNLGEPPVQIGRDNLGSSESCSSILLDYVNDQSKQLKSDLQAEQSNSLKSMRLKSLLERIEKIRVQLLEELKAGESGASKGDNAQELNNIRQERADILSERTRTLNERESDLQQKEAILEQRLRKFYKETKNAKSSESDKPKSNAKEDGPVEIIIKVRSDGTVKQYVPRTKSKAKTKPSAIDNEKDVPLGSTDSTPRDDEPKPTEQDHGKRPFLQDQRQISIDSNSTAYRSLPPVSYKNMTTAAPPSAPLHPMVVQYINRLLGMTRQNIDEMGVSSSCVTTPSTSVINSLRNVTPCPEPEAGRAERDQDENTEMNEHRMERVQTFIADNRSLINDLEESIRCQQQLQREQQSLDAEKSMRAFDQIWNKRLAKNPEDCQQTNKEKEQTREKMPRERPTPQGDRKNAAPPQESGKIRRTQEGDRQQQTNGAVQQKREQKTVKQGSGADKSTNRFAPEKSKALSTSEESSARNMERYAQLTENCTQRIAELTELITKVREEKQRLVEVTLTSNSDGERQSTEYLELPTGQQQTRCRTISDRSDSQTPSTSEALPLQKHKPTAASRDSGIADSRPITAQGQVCIDVEPISLGSSTQNNTARGRVRAPPATIRRYSPQLNAEDLAHELSTITEVETPGQSHIVAATPVPKPFPSFDQYAKELHLDLSRLDADQSQRLQGEFNDLILAIRQRNQGTDYREFPSINAYLHNMTSTRIHVEIGQDADQATLSPGELMRQLRVITHSIQDFPKRREYFEKLMAKQPPDQRDLIDSASLENDSTDSFNVEEELRQRKILKNSFRRGNATETTLTAQEVASSTRRESVAPVTNDQPNESGIDPLSGSNFSSDADQRSPCWHATMHERQQQVDELCSSTTASSPERRPRKSRLRGGHEHNNRSKDDSTVQDASQIGRSLNLREFLTRELLKHRVHDDVASESSDESLRGHFLKSVLHSLSPSNNTQTPGVGVSHATGATNDRQKTSTPVGSFLSIPEKAGSMSNTGSQLFSGESRISLVNYPDGTPPIPFEQQSKLSTNQTRQSSGQIGSGVRTTHRKSPRK

Target ORF information:

RefSeq Version	NM_143011.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster anastral spindle 1 (ana1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143011.3

ORF Insert Sequence:

ATGGCTCTGC AGCTAACAGT AAATGGAAAG TTCGGGGCCA ACCTGAGGAG GACCATAGGA 
CCACTGGACG ATCCAGAGCA AGTACGCAAA GATCTCGAAA GTGAGCGTCG CATAACCCGT 
TTGAAGCAGG TGCGGGAGAA GTCAAACCAC CTGGCACGCC AGATACGCGA GGATGTGGCT 
GCCGAAAAGA GGCGTCAAAT CCACAAATTA GAGCAGGTCA AGCAAAGGGA GCTAAATGCG 
TGGCGTGAGC ATGTTTTGGT CAAAAAGCAC CAGGACTACC GTTCGGCCAT TTTCCAGGTG 
GGCGCCGCCC ATCGCGCCGC AAAGGAGGAG AACGAACGCA TCGAGCAGCA GAGGCAAGAG 
AGAATCGACA AAATCCGAAG GTGTCGCAAG CAGGCCTTGA AAAGATCGGC TAAAGCCAGC 
GTTGAGTTGC GGACCACAAA CACAATGAAT TTAAATGCGG AGGGACGTGC CTCGGCGGGA 
ACACAAACGC CCATAGTGGA GGACAAAGAA AACCGAATGG AGAGCGGGTG TAACAAACCC 
TGTTGCAAGG GCAAGCGCAA AAGGAAAACC AGCTGTGGAT GTGCCAGCAC CGATGCAGAG 
GATGAGAAGG AGGATGACGC ATCGTCCGAT GACGACTCAT CCATCCTGAT CATCGAGAGT 
CCGAGCAGCA ACCGGCGTCT CCAAAAAACT ACCCCCGTAA TCCTAGACGT AGAAATCGAG 
GAAACCATTT CTGAAACCGA CAAGAATCCC GAGGGTATGG ATATAAATGA TAGGTTCATG 
CAAACCAACC GGAAATTCAG CCATGTCGTG CGACCCAGTG ATGATGAACC CCAACGCCGG 
CCGAGATTCA CTCAGATCAG CGATTTGGTA AGAAAAACCG AGACCACCGT ACGAGCTGGG 
CCAAGTCAAC GAAGGGAAGC AGATCCTACT CCACCAGTAT CTGCCCCGCC TAGTCCCACC 
AAATCCCCGC CAAGATCGCC CAGAAAATGT GTAGAGCGTG AGGAGCCCGT TCCTGCTCCA 
GCACCCAGAA AATCACCCAC TAAAACAGCT AAAGGATCGC CGAAGAAAAC TGTGACGGCA 
CCACGTAACC AGCCATCAGT CAAAAGAACG GGTTTAAAAT TAAATCCAGC TCCTGCCAAG 
GTGATAGATG CGGGAATACA CAGAGGTCAG AAGGCCAAAG CTCCAGCTAC GCTGCCCAAA 
GTAACAGCTA TACAGAAACC AGCAGTGGAA CCTCCTCGAA ACCTTCCACC GATTCCAGAA 
CAGGCTATGC CAGCTGTGCC AAATCCGTCG ATGACCCATT GTTATCCCAT GCCGCAGAAT 
CAACCATACA TGCATCCGTA TGCTCAACTT CCGATGCAAC CATATGCGAT GCCATATTCA 
ATGCCATTTC CCGTGCAAGC CCAGTTTCCA CAACCTCATC CGATGTATGC ACCGCAGCAA 
ATGATGCCGA ATCAGCCAGC TGTACTCGGT CCCGCTATTA CTGCTCCACC GAGCACTGCT 
ACGCAATCCA CTGTGTCCAC CACCACAATT ACCATGTCCT CGCGACAGGA CCCAAGGACG 
ACTCAATGTG GGCGTGTTCA GTTCTATGAT CATAGCAATA AGTACCATAG AACGTACGAA 
GCACCAACAC AGTCGGTGCA GTGCAATGAG AAGGATGCCA CCCAATTGAC CGCTATGGAT 
CATGCTCGCA TCGAGAACCA GCTGAGGGAT CTGCGGGAAC AGGAACTGGA CAAACTGAGG 
AAAATTAGTG ACGATCGTGG TCAAAAGGCT CTTGAACGCG AGCAAGTGCG TCGGGATTGT 
GCTGAATTAA CGGAAAAGCT AGATGCTCTA ACGCAACAGC AACCGCAGCT CTTGCCAACC 
GATGCTAACA TTATACCTAG CCATCGGTAC GCGGATGCAG CTGCCAGGCG AGAGCAAAAG 
ATGAACGATG CAATGGAAGA AATGCTGCTT CGACCAGCCA TTATCACCTG CCCGGAAGTG 
CGCACCAAAC CTACTCCTCC AAACTTATCG CGCAGCAAAT CCAAAGCTTC TGTGGCAGTT 
AATTTGGGCG AACCTCCTGT TCAAATCGGC CGGGATAATC TTGGCAGCTC GGAGAGCTGC 
AGCTCCATTC TTCTCGACTA CGTTAATGAT CAAAGCAAGC AACTCAAGTC GGACCTGCAG 
GCCGAGCAGT CAAACTCACT GAAATCGATG AGGCTAAAGA GTTTGCTTGA GCGCATTGAG 
AAAATTCGAG TCCAGCTGTT GGAGGAACTG AAGGCGGGTG AGTCAGGTGC ATCAAAGGGT 
GACAATGCAC AGGAATTGAA TAATATACGT CAGGAGCGAG CTGATATTCT ATCCGAGCGA 
ACGAGAACTT TAAACGAACG AGAATCTGAT TTGCAACAGA AGGAGGCTAT TCTCGAGCAG 
CGTCTAAGAA AGTTCTACAA GGAGACAAAA AACGCAAAAT CCAGTGAGAG TGATAAGCCA 
AAATCAAACG CAAAAGAAGA TGGGCCAGTT GAGATCATTA TTAAGGTGAG GAGTGACGGT 
ACTGTTAAGC AATATGTACC GAGAACAAAA TCGAAAGCCA AAACCAAGCC GTCGGCTATC 
GACAATGAAA AGGATGTGCC CCTGGGCTCA ACTGACAGCA CACCAAGAGA CGATGAGCCA 
AAGCCGACGG AACAGGATCA CGGAAAGCGA CCATTCTTAC AGGATCAGCG TCAGATATCC 
ATTGATTCCA ACTCCACTGC CTATCGCAGT CTGCCGCCAG TGAGCTATAA AAATATGACC 
ACGGCCGCTC CCCCTTCTGC TCCATTACAT CCCATGGTGG TTCAATATAT AAATCGTCTG 
CTAGGAATGA CACGCCAAAA TATCGATGAG ATGGGTGTGA GTTCCTCGTG TGTGACCACA 
CCATCAACGT CGGTGATCAA CTCATTGAGA AACGTAACCC CTTGTCCCGA GCCTGAAGCG 
GGAAGAGCTG AAAGGGATCA GGATGAAAAT ACGGAGATGA ACGAGCATCG CATGGAGCGG 
GTGCAAACCT TTATTGCCGA CAATCGCAGC TTAATCAATG ACTTGGAAGA ATCAATACGT 
TGCCAGCAGC AGCTGCAAAG GGAGCAACAA TCGCTGGATG CAGAGAAAAG TATGCGCGCA 
TTCGATCAAA TATGGAACAA GCGTTTGGCC AAGAATCCTG AAGATTGTCA GCAAACCAAC 
AAAGAGAAAG AGCAAACAAG AGAAAAGATG CCAAGGGAAA GGCCGACTCC ACAAGGAGAT 
AGGAAAAATG CAGCACCTCC ACAGGAAAGC GGCAAAATTC GTAGAACTCA AGAAGGGGAC 
CGGCAGCAGC AAACAAACGG TGCAGTGCAA CAAAAAAGGG AGCAAAAGAC TGTAAAACAA 
GGTTCAGGCG CCGATAAAAG CACAAACCGC TTTGCCCCCG AAAAATCGAA AGCACTATCC 
ACCAGTGAGG AGTCATCTGC CCGAAACATG GAACGCTATG CACAGCTCAC GGAGAACTGC 
ACCCAGCGGA TCGCCGAACT TACAGAGCTG ATCACGAAAG TGCGCGAGGA GAAGCAGCGA 
CTGGTGGAGG TCACCCTCAC ATCGAACAGC GATGGCGAGC GGCAGTCCAC TGAATATCTT 
GAGCTTCCAA CAGGACAACA GCAAACCAGA TGCCGGACAA TTTCCGATCG CAGCGATTCT 
CAAACACCTT CGACCTCGGA AGCCTTGCCG TTGCAGAAGC ACAAGCCGAC AGCGGCCAGC 
AGGGATAGTG GCATTGCGGA CTCACGTCCA ATTACGGCAC AGGGTCAGGT TTGTATCGAT 
GTGGAACCCA TCTCGCTTGG GTCATCAACG CAGAATAATA CAGCACGCGG ACGAGTAAGG 
GCACCTCCGG CCACGATTCG TCGGTATAGT CCTCAACTAA ACGCCGAGGA TCTGGCGCAC 
GAGCTGTCGA CCATTACAGA AGTGGAAACG CCAGGACAAT CACATATTGT GGCCGCCACA 
CCGGTGCCCA AGCCATTCCC TAGTTTCGAT CAGTATGCCA AGGAACTGCA CTTAGATCTG 
TCGCGGTTGG ATGCTGATCA GAGCCAACGG CTGCAGGGCG AATTTAATGA CTTAATACTC 
GCGATCAGGC AACGCAATCA AGGAACTGAC TACCGTGAGT TTCCTAGCAT TAATGCCTAT 
CTTCATAACA TGACTAGCAC CCGAATCCAT GTGGAGATAG GTCAGGATGC AGATCAGGCC 
ACTCTGTCAC CGGGTGAACT GATGCGACAG CTGCGCGTGA TCACTCATAG TATCCAAGAC 
TTTCCCAAGC GCAGGGAATA CTTTGAAAAA CTTATGGCCA AGCAACCACC CGATCAGAGG 
GATCTAATAG ACAGTGCTAG CTTAGAGAAT GATTCCACGG ATTCATTTAA CGTAGAGGAA 
GAACTCCGCC AGCGGAAAAT TCTAAAGAAC TCATTTCGTC GAGGAAACGC AACTGAGACC 
ACATTGACGG CTCAGGAGGT GGCTAGCAGT ACGCGGCGGG AAAGCGTCGC CCCAGTGACC 
AACGATCAAC CCAACGAGAG TGGCATAGAT CCCCTCTCCG GCAGCAACTT TTCCAGCGAC 
GCGGATCAGA GAAGTCCGTG CTGGCATGCC ACAATGCATG AGAGGCAACA GCAGGTGGAT 
GAGTTGTGCA GCAGTACCAC AGCCTCGTCG CCAGAACGGC GGCCACGAAA GTCTCGGCTT 
CGTGGAGGCC ACGAGCACAA CAATAGGTCT AAGGACGATT CCACTGTGCA GGATGCAAGC 
CAGATAGGAA GATCCCTTAA TCTGAGGGAA TTCCTTACGA GAGAGCTCCT CAAACATCGA 
GTGCACGACG ACGTTGCCAG TGAGAGTTCA GATGAGTCCT TAAGGGGGCA CTTTCTGAAG 
TCGGTCCTAC ATTCGCTCTC GCCCAGCAAC AACACGCAAA CGCCAGGAGT GGGTGTAAGC 
CATGCCACCG GAGCAACAAA CGACAGACAA AAGACATCCA CACCAGTGGG ATCGTTCCTC 
TCCATTCCAG AGAAGGCCGG GTCCATGAGT AACACCGGCT CTCAACTGTT CTCCGGGGAG 
AGTCGCATCT CCCTGGTTAA CTATCCAGAT GGAACTCCAC CCATTCCTTT TGAACAACAA 
AGCAAACTTA GTACAAATCA AACTCGCCAA TCCTCAGGTC AAATAGGATC AGGTGTCAGG 
ACAACGCATC GAAAGTCGCC CCGAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ana1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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